CHEMICO-BIOLOGICAL INTERACTIONS
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2021
·
IF 5.194
Min Wang,Longji Fang,Tongtong Liu,Xuejie Chen,Yan Zheng,Yilong Zhang,Shiming Chen,Zeng Li
The development of multi-target-directed ligands (MTDLs) may improve complex central nervous system diseases such as Alzheimer's disease (AD). Here, a series of 7-O-1, 2, 3-triazole hesperetin derivatives was evaluated for their inhibition of cholinesterase , anti-neuroinflammatory, and neuroprotective activity. Among the hesperetin derivatives, compound a8 (7-O-((1-(3-chlorobenzyl)-1H-1,2,3-triazol-4-yl)methyl)hesperetin) possessed excellent anti-butyrylcholinesterase activity (IC 50 = 3.08 ± 0.29 μM) and exhibited good anti-neuroinflammatory activity (IC 50 = 2.91 ± 0.47 μM) against NO production through remarkably blocking the NF-κB signaling pathway and inhibiting the phosphorylation of P65. In addition, a8 showed a remarkable neuroprotective effect and lacked neurotoxicity up to 50 μM concentration. Furthermore, possessing significant self-mediated Aβ 1-42 aggregation inhibitory activity, chelated biometals and reduced ROS production were found in compound a8 . In the bi-directional transport assay, a8 exhibited a blood–brain barrier penetrating ability. In this study, the Morris water maze task showed that compound a8 significantly improved the learning and memory impairment of the scopolamine-induced AD mice model. Results highlighted the potential of compound a8 to be a potential MTDL for the development of anti-AD agents.
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高影响因子
ACS Sustainable Chemistry & Engineering
·
2021
·
IF 8.198
Kemin Lv,Zhimeng Yu,Marcelo M. Pedroso,Bin Wu,Zhen Gao,Bingfang He,Gerhard Schenk
Establishing a suitable immobilization strategy to improve the accessibility of immobilized cellulase for insoluble cellulose and subsequently recover the enzyme from the remaining substrate is crucial to promote the industrialization of biofuels. Here, using iminodiacetic acid (IDA) and Ni2+ ions, a novel metal-chelated copolymer of methacrylic acid and methyl methacrylate was prepared for the immobilization of His-tagged processive endoglucanase EG5C-1 via affinity interaction between polyhistidine tag and Ni2+. The optimum loading capacity of the functionalized polymer (Eud-IDA-Ni2+) for EG5C-1 was about 280 mg/g, where more than 80% of the activity was recovered. Immobilized EG5C-1 exhibited improved thermal and pH stability and better reusability than the free one, and after five cycles of usage, the hydrolysis productivity remained above 70% of the initial value. The Eud-IDA-Ni2+/EG5C-1 biocomposite displayed reversibly soluble–insoluble characteristics with pH change, which was in the soluble state during the enzyme reaction process but could be recovered in an insoluble form by lowering the pH after the reaction. Thus, the yield obtained from the hydrolysis of an insoluble phosphoric acid-swollen cellulose substrate was similar for the free and immobilized form of EG5C-1. Our combined results suggested that a metal-ion-chelated, pH-responsive polymer had the potential for His-tagged cellulase immobilization to improve both the operational efficiency and economic benefit of the cellulosic biorefinery industry.
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高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2021
·Vol. 168
·期 5
·057509
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IF 4.316
Yuan Li,Yutong Li,Chao Du,Pengju Zhang,Peipei Cen,Xinsheng Liu,Yonghong Li
Urinary 1-hydroxypyrene (1-OHP) is generally used as an exposure biomarker of polycyclic aromatic hydrocarbons (PAHs). In this work, a colloidal gold modified zeolitic imidazolate frameworks-67/carbon nanofiber paste electrode (Au@ZIF-67/CNFPE) was fabricated for the rapid and sensitive detection of 1-OHP. The morphologies of carbon nanofibers (CNF), ZIF-67, colloidal gold and colloidal gold@ZIF-67 (Au@ZIF-67) were studied by transmission electron microscopy (TEM). The electrochemical behavior of 1-OHP was investigated by square wave voltammetry (SWV). The effect factors like pH value, amount of colloidal gold, accumulation potential and accumulation time have been optimized. Under the optimal conditions, the constructed sensor responded to 1-OHP linearly in the concentration range of 0.0005–10 μM, and the detection limit was 0.1 nM. The reproducibility, stability and selectivity of this sensor were also examined. Finally, the electrode was successfully applied for the analysis of 1-OHP in human urine samples.
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BIOMATERIALS
·
2021
·
IF 12.479
Xueqing Zhou,Xuan Liu,Xiqin Yang,Li Wang,Yiling Hong,Keke Lian,Guoxi Qiu,Xuwei Shang,Zhongjun Ma,Hong Yuan,Fuqiang Hu
Tumor-associated macrophages (TAMs) in the tumor microenvironment (TME) play an important role in the development of tumors by secreting a variety of cytokines or directly communicating with tumor cells, making TAMs-targeted therapeutic strategies very attractive. It has been reported that oncogene c-Myc is related to every aspect of the oncogenic process of tumor cells and the alternative activation of macrophages. Hence, we constructed a glycolipid nanocarrier containing ROS-responsive peroxalate linkages (CSOPOSA) for ROS-triggered release of drugs and further modified it with Ex 26 (Ex 26-CSOPOSA), a selective sphingosine 1-phosphate receptor 1 (S1PR1) antagonist, to achieve the dual-targeted delivery of the c-Myc inhibitor JQ1 via S1PR1, which is overexpressed on both tumor cells and TAMs, thereby inducing apoptosis of tumor cells, and blocking M2 polarization of macrophages. More strikingly, our studies found that JQ1 could effectively inhibit the migration of tumor cells induced by M2 macrophages-derived exosomes via blocking Caveolin-1 related intercellular exosome exchange through lncRNA H19 and miR-107. The in vivo results revealed that this dual-targeted delivery strategy effectively inhibited tumor growth and metastasis with less systemic toxicity, providing a potential method for effective tumor treatment.
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PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2021
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IF 13.273
Qihang Yu,Wu Tang,Yang Hu,Jian Gao,Ming Wang,Sihong Liu,Huanhuan Lai,Liang Xu,Cong Fan
For the first time, two novel air-sensitive Li-rich organic cathodes ( m -TP4OLi and p -TP4OLi) are designed and reported. The structure-performance relationship for the two organic cathodes is comparatively studied. Using organic anode of lithium terephthalate (Li 2 TP), the resulting Li 2 TP II p -TP4OLi all-organic Li-ion batteries can deliver the peak discharge capacity of 330 mAh g −1 cathode in 1–3.4 V, corresponding to the high energy density of ~574 Wh kg −1 cathode using the median voltage of 1.74 V. Furthermore, the fabricated graphite II p -TP4OLi Li-ion batteries can show the peak discharge capacity of 305 mAh g −1 cathode in 1.5–3.4 V with a high median voltage of 2.4 V, resulting in the peak energy density of ~732 Wh kg −1 cathode (2.4 V × 305 mAh g −1 ). Meanwhile, the graphite II p -TP4OLi Li-ion batteries exhibit impressive device stability over 20,000 cycles. To the best of our knowledge, the peak energy density of our p -TP4OLi is currently the world record among Li-rich organic cathodes in lab conditions, outperforms the energy density of inorganic LiFePO 4 (~476 Wh kg −1 ), comparable with that of inorganic LiCoO 2 (~760 Wh kg -1 ) under the same lab conditions.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2020
·
IF 3.99
Deng Wang,Jiaxin Yang,Fei Xue,Jihui Wang,Wenbin Hu
Zinc coordinated 1-hydroxyethylidene-1,1-diphosphonic acid (Zn-HEDP) film was fabricated on steel surface by coordination self-assembly method. The surface morphology and chemical composition of Zn-HEDP film were investigated by scanning electron microscopy (SEM) and energy dispersive spectrometer (EDS), and the structural and valence state of Zn-HEDP film was determined by Fourier transform infrared spectroscopy (FTIR), Raman spectra and X-ray photoelectron spectroscopy (XPS). The corrosion resistance of Zn-HEDP film in 3.5wt% NaCl solution was investigated by using potentiodynamic polarization curve and electrochemical impedance spectroscopy (EIS) techniques. The experimental results show that Zn-HEDP film is in a smooth surface with disturbed nanoparticles. The protection efficiency of Zn-HEDP film to steel in 3.5 wt% NaCl solution is firstly increased and then decreased with the increasing of zinc concentration, pH value and assembly time. The optimum preparation conditions for Zn-HEDP film are the zinc concentration of 10 mmol/L, pH value of 5.0 and assembly time of 30 min, under which the protection efficiency of film is reached to 95.79 %. The coordination between zinc ions and HEDP can effectively increase the binding energy with Q235 steel. Zinc ions can improve the compactness and thickness of Zn-HEDP films as bridging agents, which prevent Q235 steel from corrosive solution.
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JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2020
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IF 3.807
Yijing Ai,Ningning Gao,Qingxiang Wang,Feng Gao,D. Brynn Hibbert,Chuan Zhao
Metal-organic frameworks (MOFs) receive increasing interest in the construction of high-performance electrochemical sensors, because of their outstanding properties such as large surface area, rich active sites, tunable pore size, and high electrocatalysis. In this work, an electroactive sensing metal-organic framework material, Hong Kong University of Science and Technology-1 (HKUST-1), has been successfully constructed by the in-situ electrodeposition of metallic copper (Cu) on an electrode surface modified with Nafion-single walled carbon nanotubes (SWCNTs-Nafion), followed by potentiostatic scan in benzene-1,3,5-tricarboxylic acid solution. The HKUST-1 modified electrode was characterized by scanning electron microscopy, FT-IR and electrochemistry. Electrochemical sensing assays show that the HKUST-1/SWCNTs-Nafion modified electrode can effectively resolve the overlapping redox signals of the three dihydroxybenzene isomers (DHBIs), namely hydroquinone (HQ), catechol (CT), and resorcinol (RS), and is capable of determination of the DHBIs in real water samples.
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高影响因子
ARCHIV DER PHARMAZIE
·
2020
·Vol. 354
·期 2
·2000165
·
IF 2.59
Yushan Huang,Hongmei Hu,Rui Yan,Liwen Lin,Mingxia Song,Xiaodong Yao
A series of 3-phenyl-1-phenylsulfonyl pyrazoles containing an aminoguanidine moiety was designed, synthesized, and evaluated for their antimicrobial and anticancer activities. The majority of the target compounds showed broad-spectrum antimicrobial activity against the tested strains, with minimum inhibitory concentration (MIC) values ranging from 2 to 64 μg/ml. Compound 5k , showing the most potent antimicrobial activity against Bacillus subtilis CMCC 63501 and multidrug-resistant Staphylococcus aureus ATCC 43300 with an MIC value of 2 μg/ml, was the most promising one in this series. It was also effective for S. aureus ATCC 33591 and multidrug-resistant Escherichia coli ATCC BAA-196 at higher concentrations. The bactericidal time–kill kinetics test illustrated that compound 5k had rapid bactericidal potential. Docking results exhibited that compound 5k showed various kinds of binding to the FabH receptor, reflecting that 5k could bind with the active site well. All compounds showed excellent activity against the investigated cancer cells, with IC 50 values ranging from 1.90 to 54.53 µM. Among them, compound 5f showed prominent cytotoxicity with IC 50 = 1.90 µM against A549 cells, while exhibiting lower inhibitory activity against 293T cells (IC 50 = 41.72 µM), indicating that it has the potential for a good therapeutic index as an anticancer drug.
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
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IF 7.1
Hui Kong,Wei Zhang,Jia Yao,Chao Li,Renfei Lu,Zhen Guo,Jinze Li,Chuanyu Li,Yingxue Li,Chiyu Zhang,Lianqun Zhou
Development of a simple, sensitive and specific method for HIV-1 assay is demanded. In some emergency situations, a portable reverse transcription loop-mediated isothermal amplification (RT-LAMP) platform is necessary. A novel hydrogen ion-selective RT-LAMP (H + -RT-LAMP) sensor was developed for highly sensitive HIV-1 using hydrogen ion concentration as a signal producing compound. The H + -RT-LAMP sensor was based on screen-printed with carbon-doped graphene conductive layer, carbon nanotubes (CNTs) solid electrolyte layer, and reference electrode KCl functional layer, which provided superior electrical signals, high sensitivity and stability for HIV-1 detection. The rate of hydrogen ion production was related to the viral load (VL) during the amplification of HIV-1. The sensitive HIV-1 RNA detection range was achieved from 0 copies/μL to 10 5 copies/μL. The proposed H + -RT-LAMP sensor was applied to detect HIV-1 RNA in real human serum. 57 samples were detected and satisfactory results were obtained. Compared with commercial instruments, the detection results of H + -RT-LAMP sensor demonstrated a perfect concordance rate of 96.5 %, and the limit of detection (LOD) for the clinical samples was 10 copies per tube. Time to get the result is from 10 min to 25 min. Therefore, the proposed H + -RT-LAMP sensor was particularly suitable as a point-of-care device for the on-site detection of HIV-1.
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2020
·
IF 4.872
Ling Ou,Haixia Wang,Zhidi Wu,Panpan Wang,Li Yang,Xiaoyun Li,Kehuan Sun,Xiaofeng Zhu,Ronghua Zhang
Cadmium is an environmental metal pollutant that has been a focus of research in recent years, which is reported to cause bone disease; however, its skeletal toxicity and the mechanism involved are not yet fully known. Therefore, this study used MC3T3-E1 subclone 14 cells to determine the mechanism of cadmium toxicity on bone. Cadmium chloride (Cd) significantly reduced cell viability in a concentration-dependent manner. Exposure to Cd inhibited osteoblast-related proteins (Runx2, Col-1, STC2) and decreased alkaline phosphatase (ALP) activity. Cd caused Exportin-1 accumulation and induced DNA damage. Cd significantly down-regulated caspase 9 and induced cleaved-PARP, cleaved-caspase 3 protein level. Treatment with JNK inhibitor, SP600125, suppressed cadmium-induced elevation in the ratio of phosphorylation of JNK to JNK. Inhibition of caspase with pan-caspase inhibitor, Z-VAD-FMK, prevented MC3T3-E1 subclone 14 cells from cadmium-induced reduction of Runx2, STC2, caspase 9, and accumulation of cleaved PARP and cleaved caspase 3. Cd-induced cell survival enhanced by SP600125 but rescued by Z-VAD-FMK or KPT-335. These results suggest that cadmium cytotoxicity on bone involved exportin 1 accumulation, phosphorylation of JNK, induction of DNA damage and pro-apoptosis, which was induced by activation of caspase-dependent pathways.
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PubMed
高影响因子
Advanced Healthcare Materials
·
2020
·Vol. 10
·期 3
·2001029
·
IF 7.367
Shuo Huang,Linlin Zhang,Miaomiao Xu,Chao Li,Hongliang Fu,Jie Huang,Xin Jin,Sheng Liang,Hui Wang
New radionuclide-labeled targeting nanocarrier systems have generated new opportunities for tumor treatment and imaging. Nevertheless, such therapeutic strategy is clinically unfeasible on anaplastic thyroid carcinoma (ATC) patients, because of lacking suitable targets and resistance to radiation. In order to figure out a potential treatment, immuno-histochemical staining is performed in human ATC tissue species and high expression of cluster determinant 44 (CD44) is found. Therefore, a CD44-targeted delivery system is designed and constructed by self-assembly of tyrosine (Tyr)–hyaluronic acid (HA)–polyethyleneimine (PEI), which can radiolabel 131/125 I and load a p53 mutant restoring regent, Prima-1. The 125 I-labeled nanocomposites display an impressive tumor imaging as well as a long radiation treatment cycle. The 131 I-labeled nanoparticles show remarkable anti ATC-tumor effects in vitro and in vivo, due to radiosensitization of Prima-1 by reactivation of the p53 mutants.
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高影响因子
LIQUID CRYSTALS
·
2020
·
IF 2.908
Shenghua Du,Mengting Zhang,Pei Chen,Jiazhen Dang,Aiai Gao,Weisong Du,Xinbing Chen,Zhongwei An
With altering molecular dipole moments via lateral fluoro atom and π-conjugation through carbon–carbon triple bond, improved mesomorphic behaviour is achieved for new kind of fluorinated mesogenic compounds containing ethynyl and 1-methyl-1H-benzimidazole moieties, namely, 2-[4-[2-[4-(alkoxy) phenyl]ethynyl]-3-fluorophenyl]-1-methyl-1H-benzimidazole derivatives (nPEF(3)PMx). The compounds nPEF(3)PMx mainly display enantiotropic nematic mesophases with mesophase ranges of 27.1–59.1 °C and 62.7–96.7°C on heating and cooling for nPEF(3)PMH, 59.499.1°C and 96.2–144.6°C for nPEF(3)PMM, and 101.6–109.4°C and 104.9–157.2°C for nPEF(3)PMN (n < 8), respectively. Compared with nonfluorinated reference analogues I, nPEF(3)PMx give obviously lower melting points and much wider or comparable nematic mesophase ranges, meanwhile, they show much lower melting points and higher nematic mesophase stability than reference analogues II because of their reduced molecular dipole moments and enhanced π-conjugation. In addition, the compounds nPEF(3)PMx are composed of benzene ring, ethynyl bridge and benzimidazole mesogenic unit, therefore, they have high birefringence of 0.48–0.62 resulted from their large π-conjugated molecule. The result indicates that nPEF(3)PMx has a potential application in liquid crystal mixture.
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