ACS Sensors
·
2020
·
IF 7.333
Xiao-Bin Huang,Shao-Hua Wu,Hao-Cheng Hu,Jian-Jun Sun
The “turn-on” mode surface-enhanced Raman scattering (SERS) aptasensor for ultrasensitive ochratoxin A (OTA) detection was developed based on the SERS “hot spots” of AuNanostar@4-MBA@Au core–shell nanostructures (AuNS@4-MBA@Au) and exonuclease III (Exo III)-assisted target cycle amplification strategy. Compared with conventional gold nanoparticles, AuNS@4-MBA@Au provides a much higher SERS enhancement factor because AuNS exhibits a larger surface roughness and the lightning rod effect, as well as an excellent electromagnetic field between the AuNS core and the Au shell, which contribute to the superstrong SERS signal. Meanwhile, Exo III-assisted target cycle amplification can be used as an effective method for the further amplified detection of OTA. Additionally, the utilization of streptavidin magnesphere paramagnetic particles offers a green, economical, and facile technology for the accumulation and separation of the signal probe AuNS@4-MBA@Au from solution. All these factors lead to a significant enhancement of detectable signals and superhigh sensitivity. As a result, the limit of detection as low as 0.25 fg mL–1 could be achieved, which was lower than that in the other reported literatures on SERS methods for OTA detection as we know. The developed SERS aptasensor also provides a promising tool for foodstuff detection.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2020
·
IF 5.065
Ling Zhou,Jie Yang,Xinyue Wang,Gao Song,Fan Lu,Lijun You,Jumei Li
A novel highly active surface enhanced Raman scattering (SERS) substrate of Ag nanoparticles decorated Ag@ZrO 2 composite nanosphere was prepared and characterized. A uniform ZrO 2 shell was firstly coated over Ag cores by sol-gel process using zirconium butoxide as Zr precursor to achieve Ag@ZrO 2 nanospheres and then Ag nanoparticles were in situ deposited on the Ag@ZrO 2 through the reduction of AgNO 3 to produce Ag@ZrO 2 @Ag composite nanospheres. The cover density of Ag nanoparticles on Ag@ZrO 2 @Ag nanospheres could be controlled by changing the concentration of AgNO 3 . TEM , FE-SEM, EDX, UV–vis and XRD analyses were used to characterize their morphology, structure and composition . The SERS property of Ag@ZrO 2 @Ag was evaluated using 4-mercaptobenzoic acid (4-MBA) as Raman probe molecules. The experimental results showed Ag@ZrO 2 @Ag nanospheres presented remarkably SERS activity, high signal reproducibility (RSD = 6.39%) and exceptional storing stability. Subsequently, the Ag@ZrO 2 @Ag nanospheres acted as SERS substrates to sense Cr (VI) in water at various concentrations, and the SERS results showed great linear correlation between SERS intensities and the concentration of Cr (VI) (R 2 = 0.97) with the detection limit of 0.5 μM, which is below the safety level (1 μM) in drinking water of United States Environmental Protection Agency. The Ag@ZrO 2 @Ag nanospheres were also successfully used to confirm the concentration of Cr (VI) in real industrial wastewater. These results demonstrated the extraordinary potential of Ag@ZrO 2 @Ag composite nanospheres serving as SERS substrates for trace and quantitative determination of Cr (VI) in environmental field.
✓ 已验证
产品关联
DOI
高影响因子
GIANT
·
2021
·
IF 0
Haolin Chen,Zhiming Liu,Ouyang Jiang,Jingyang Zhang,Jun Huang,Xinru You,Zhiqing Liang,Wei Tao,Jun Wu
To expand the bioimaging applications of Au and black phosphorus, black phosphorus quantum dot-Au hybrids (Au-BPQD nanohybrids) are fabricated for multiple theranostic applications in this study. By simply varying the ratios of BPQDs and Au 3+ , the BPQDs assisted Au 3+ reduction outputs two kinds of nanohybrids with different functions. The less BPQDs lead to small size Au-BPQD nanohybrids (S-ABPs) with a core-shell structure. S-ABPs exhibit superior near-infrared (NIR) surface-enhanced Raman scattering (SERS) activity for the fingerprint analysis of biomolecules. It's also very promising for interference-free bioimaging due to the distinctive Raman band at around 2190 cm −1 in the biologically Raman-silent region, which cannot be achieved by Au or BP-based nanomaterials. Meanwhile, further addition of BPQDs would lead to large size Au-BPQD nanohybrids (L-ABPs) with strong NIR optical absorption, which facilitates the in vivo 3D photoacoustic imaging as well as the photothermal therapy against tumor. This work reports versatile nanotheranostic platforms for amphibious SERS spectroscopy, 3D photoacoustic imaging and cancer therapy, which opens a new door for the biomedical applications of BP and Au based nanomaterials.
✓ 已验证
产品关联
DOI
BIOSENSORS & BIOELECTRONICS
·
2019
·
IF 9.518
Xiang Pan,Linhu Li,Hangduo Lin,Jiayi Tan,Haitao Wang,Mengling Liao,Caiju Chen,Beibei Shan,Yingfan Chen,Ming Li
We report a paper-based surface-enhanced Raman spectroscopy (SERS) biosensor integrating the enrichment capability, namely enPSERS biosensor, for the sensitive, label-free detection of free bilirubin in blood serum for the accurate diagnosis of jaundice and its related diseases. This biosensor comprises multifunctional graphene oxide-plasmonic gold nanostar (GO-GNS) hybrids decorated on the filter paper, which integrates the high sensitivity of SERS detection, enrichment for serum bilirubin and fluorescence superquenching capability of GO-GNS hybrids for sensitive detection of serum bilirubin. The study of adsorption kinetics reveals that both electrostatic and π-π interactions between the GO-GNS hybrids and targets are responsible for the enrichment of bilirubin, and the adsorption process follows the pseudo-second-order kinetic model . The results of SERS detection of bilirubin in blood serum show two differential linear response ranges from 5.0 to 150 μM and 150–500 μM with the detection limit as low as 0.436 μM. The comparison of the results obtained from our present enPSERS biosensor with the commercial diazo reaction method for determination of free bilirubin in blood serum reveals the clinical effectiveness and suitability of the developed paper-based SERS biosensor. We believe that this sensitive and label-free SERS biosensor holds considerable promise for clinical translation in accurate diagnosis of jaundice.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2019
·
IF 3.206
Huanhuan Li,Xiaoqian Huang,Md. Mehedi Hassan,Min Zuo,Xiangyang Wu,Yiping Chen,Quansheng Chen
Here, we proposed a novel dual-channel biosensor for rapid and sensitive detection of Hg 2+ . Compared with the previous biosensors, the [email protected] /graphene-upconversion ( [email protected] ) was prepared via a facile method and acted as a versatile signal indicator that can simultaneously readout surface-enhanced Raman spectroscopy (SERS) and fluorescence signals for Hg 2+ detection. In this strategy, the core shell magnetite colloid nanocrystal clusters-polymethacrylic acid magnetic beads (MCNCs/PMAA MBs) were synthesized and conjugated with aptamer to specific capture the Hg 2+ . The dual-channel biosensor was thus fabricated by immobilizing the [email protected] onto the surface of MBs through the complementary pairing of aptamer and complementary DNA (cDNA). Upon Hg 2+ incubation, Hg 2+ preferably bound to the MBs-aptamer, resulting in the subsequent release of preloaded [email protected] into supernatants. Under magnetic attraction, the MBs-aptamer was accumulated immediately, resulting in the concentration of Hg 2+ become homodromous proportional to the dual channel intensity of the supernatants. Under the optimized conditions, the dual channel biosensor achieved excellent performances for Hg 2+ with limits of detection (LOD) of 0.33 and 1 ppb, respectively. Furthermore, the feasibility of the biosensor to qualify Hg 2+ was also established in spiked tap water and milk samples. This strategy may provide a bridge between a highly sensitive fluorescence assay and a rapid SERS assay, it can broaden the applicability of Hg 2+ detection, which can be easy to detect Hg 2+ in real samples by coupling with SERS or fluorescence assay.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF RAMAN SPECTROSCOPY
·
2019
·Vol. 50
·期 10
·1445-1451
·
IF 2.809
Aili Liu,Huile Jin,Jun Li,Liyun Chen,Haoyuan Zheng,Xinnan Mao,Dajie Lin,Jichang Wang,Shun Wang,Weizhong Jiang
A microwave- and ultrasound-assisted reduction and assembly of lead dimethyldithiocarbamate (Pb (DMDC) 2 ) led to the formation of rod-like Pb (DMDC) 2 embedded with PbS quantum dots (QDs). Transmission electron microscopy measurements show the presence of numerous isolated PbS QDs on the Pb (DMDC) 2 microrod surface, which may potentially act as hot spots for enhanced Raman spectroscopy. A case study using mercaptopyridine as the analyte demonstrates that the rod-like hybrids have outstanding performance as a surface-enhanced Raman scattering (SERS) substrate, comparable with that of Au nanorods. The excellent SERS performance of the as-fabricated PbS QDs/Pb (DMDC) 2 hybrids may be attributed to the unique dispersion of PbS QDs as well as the intimate contacts between PbS QDs and Pb (DMDC) 2 . This study therefore presents a new approach for the development of low-cost and highly efficient semiconductor-based SERS substrates.
✓ 已验证
产品关联
DOI
高影响因子
APPLIED SURFACE SCIENCE
·
2019
·
IF 5.155
Li Zhang,Xiaodan Li,Yunyu Jin,Youlin Zhang,Xiaomin Liu,Yulei Chang,Lijun Xu,Huiying Zhao,Langping Tu,Dan Wang,Xianggui Kong,Bin Xue
The advances in surface-enhanced Raman scattering (SERS) have resulted in significant improvements for sensing glucose, achieving a highly detecting sensitivity of the order of μM. Highly sensitive glucose detection, however, is still a challenge due to extremely small Raman scattering cross section of glucose molecules and weak interaction between the molecules and the metal nanoparticles . Here, we designed a novel homogeneous SERS-based nanoplatform composing of the SERS tags and glucose oxidase in aqueous solution for a highly sensitive glucose detection. Adding glucose caused enzymatic oxidation reaction to etch the tip electric field ( E ) of silver nanotriangle. Thus, the decreased intensity of E was greatly amplified by the change of SERS signal ( E 4 dependent), which resulted in an excellent glucose detection limit of 0.4 nM was firstly achieved. Furthermore, the nonlinear relationship between the decreased SERS intensity and the glucose concentrations was reasonably revealed based on theoretical calculations and deeply understanding of the enhancement mechanism, which is very useful in improving the sensitivity and accuracy of trace glucose detection. The as-prepared nanoplatform showed good sensitivity and high selectivity only through observing the SERS intensity change, which is potential for highly sensitive and practical glucose detection for clinical trace analyses.
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Xia Zhou,Qian Zhao,Guangqiang Liu,Weiping Cai
Active capture and ultrasensitive SERS-based detection of hexachlorocyclohexane (HCH) has been a challenging task. A novel strategy based on 4-mercaptophenylboronic acid (4-MPBA) surface modification on Au nanosheets-built hollow sub-microcubes (ANHCs) was proposed to capture HCH pesticides effectively via Suzuki cross coupling reaction. The ANHCs structure composed of numerous intertwined nanosheets was firstly prepared by Au nanocrystals heteroepitaxial growth on AgCl cubes. Then, the 4-MPBA molecules were selected to modify on the surface of ANHCs for SERS-based detection of HCH. The SERS results proved that active capture and ultrasensitive detection of HCH could be implemented, and the limit of detection is 0.3 ppb. Besides, 4-MPBA modified substrate show high reproducibility and sensitivity than unmodified one. Moreover, the characteristic peak intensity ratio I HCH /I 4-MPBA presents a good linear relationship with the logarithm of a certain concentrations, and achieve SERS-based quantitative detection of HCH. In addition, this strategy has been successfully used for the detection and differentiation of mixed HCH isomers in water samples. The surface modification strategy on high performance SERS substrate provides a good route for the highly ultrasensitive detection and active capture of HCH, and paves a way for the detection of organochlorine pesticide residues in water samples.
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 46
·36368-36373
·
IF 3.84
Peilan Wang,Yanling Zhou,Ying Wen,Feng Wang,Haifeng Yang
Fabrication of cheap and disposable substrates with high surface enhanced Raman scattering (SERS) effects is essential for practical SERS application. In this study, versatile substrates (silica plate, polyethylene film and filter paper) were simply modified with polydopamine followed by in situ silver nanoparticle deposition to fabricate a two-dimensional SERS substrate. This kind of SERS substrate has good reproducibility (relative standard deviation (RSD) <8% (spot-to-spot) and <12% (batch-to-batch)) and stability (>89% after 2 months). Using 4-mercaptopyridine (4-Mpy) as the probe molecule, the enhancement factor could reach 107. This facile and general approach can be easily scaled up to fabricate cheap, easily portable, flexible and disposable SERS substrates, which can be applied for on-site SERS detection.
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2014
·
IF 4.058
Jiale Huang,Dengpo Huang,Yi Liu,Huimei Chen,Xiaolian Jing,Daohua Sun,Qingbiao Li
Gold recovery is of practical significance while functional Au nanostructures are of great interest in modern nanotechnology. In this work, highly and hierarchically branched Au nanowires (AuNWs) were produced to obtain bio-AuNW composites while the Au was effectively recovered from aqueous HAuCl 4 solution by a microorganism-mediated, surfactant-directed (MSD) approach with Lactobacillus spp. cells (LSCs). Ternary coordination of the cell-surfactant-AA played an important role in recovering Au and producing the bio-AuNW composites in the cases of tetradecyl trimethyl ammonium bromide (TTAB), hexadecyltrimethylammonium bromide (CTAB) and octadecyl trimethyl ammonium bromide (OTAB). In contrast, Au recovery could not be achieved with hexadecyltrimethylammonium chloride (CTAC). Strong interaction between [AuCl 4 ] − and the LSCs prior to addition of surfactant (except CTAC) and ascorbic acid (AA) was vital for the highly and hierarchically branched nanostructures. Furthermore, the bio-AuNW composites could serve as excellent surface-enhanced Raman spectroscopy (SERS) substrate. the SERS detection limits of toxic rhodamine 6G (R6G) and 4-mercaptobenzoic acid (MBA) with the bio-AuNW composites are down to 10 −9 and 10 −6 M, respectively. This work therefore exemplifies combining recovery of Au and fabrication of functional Au nanomaterials, which was environmentally benign and application-oriented.
✓ 已验证
产品关联
DOI
高影响因子
Nanoscale
·
2016
·Vol. 9
·期 4
·1599-1606
·
IF 7.76
Jian Zhang,Lifang He,Peirong Chen,Chao Tian,Jianping Wang,Bianhua Liu,Changlong Jiang,Zhongping Zhang
Chemical sensing for the convenient detection of trace aqueous fluoride ions (F−) has been widely explored with the use of various sensing materials and techniques. It still remains a challenge to achieve ultrasensitive but simple, rapid, and inexpensive detection of F− for environmental monitoring and protection. Here we reported a novel surface-enhanced Raman scattering (SERS) nanosensor, fluorescein phenylboronic acid covalently linked to 1,4-dimercapto-2,3-butanediol modified Au@Ag NPs by a cyclic boronate ester (Flu-PBA-Diol-Au@Ag NPs), for the rapid and ultrasensitive detection of F−. Once the Flu-PBA approached the surface of Au@Ag NPs, the Raman signals of Flu-PBA were remarkably enhanced due to the strong SERS effect. However, the presence of F− will induce the cleavage reaction of the cyclic boronate ester into the trifluoroborate anion (3F-Flu-PBA) and diol. The 3F-Flu-PBA molecules exfoliated from the surface of Au@Ag NPs, and the SERS signals of the nanosensor were quenched. Following the sensing mechanism, a silica-based SERS chip has been fabricated by the assembly of Flu-PBA-Diol-Au@Ag NPs on a piece of silicon wafer. The silica-based SERS chips showed high sensitivity for aqueous F−, and the limit of detection (LOD) could reach as low as 0.1 nM. Each test using the SERS chip only needs a droplet of 20 μL sample and is accomplished within ∼10 min. The silica-based SERS chip has also been applied to the quantification of F− in tap water and lake water.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2016
·Vol. 8
·期 42
·7587-7596
·
IF 1.915
Shaojie Jia,Dan Li,Essy Kouadio Fodjo,Hu Xu,Wei Deng,Yue Wu,Yuhong Wang
Synthesis of high-quality metal nanoparticle (NP) decorated graphene (GN) with uniform and controllable nanostructures has attracted considerable attention due to its excellent electronic structure and optical properties. Herein, we develop a facile and robust process for tailoring hexanethiol (SH)-modified Ag NP-decorated graphene nanomaterials (Ag/GN-SH) by coupling in situ reduction and the covalent assembly technique. The as-prepared Ag/GN-SH combines adequate Raman “hot spots” among the high-density Ag NPs and the excellent adsorption performance of GN, making it an excellent surface-enhanced Raman scattering (SERS) substrate for highly selective and sensitive detection of PAHs. Moreover, the Ag/GN-SH can be used as a solid-phase extraction (SPE) adsorbent for qualitative and quantitative detection of PAHs in seawater from their discriminant SERS peaks. The Ag/GN-SH-based SPE-SERS is effective over a wide range of concentrations (0.1 nM to 0.5 mM) for PAHs, with tens of pM detection limits [signal to noise ratio (S/N) = 3], and the detection concentration deviation is less than 15% between the SPE-SERS and GC-MS. This study not only offers a new method for on-site removal of PAHs but also provides a strategy for simultaneous multiplexed detection of PAHs.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）