ACS Applied Materials & Interfaces
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2023
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IF 9.5
Dawei Cheng,Ruyuan Ding,Xin Jin,Yifan Lu,Weimin Bao,Yang Zhao,Shi Chen,Cailiang Shen,Qing Yang,Yuanyin Wang
Critical-size bone defects are an important problem in clinical practice, which usually occurs in severe trauma, or tumor resection, and cannot heal completely and autonomously. Implantation of grafts is often required to promote the regeneration of critical-size bone defects. Metal ions play an important role in human health, as they affect the body’s metabolism and the tissue function. Strontium ions (Sr2+) can promote osteogenesis and angiogenesis. Herein, we prepared nano-hydroxyapatite (nHA)/chitosan (CS) composite microspheres with a uniform particle size distribution and an extracellular matrix-like nanofiber structure using microfluidic technology and direct alkali-induced gelation. Strontium ions were stably added into the microspheres by using polydopamine (PDA) to chelate metal ions forming a bone repair material (nHA/CS@PDA-Sr) with good bioactivity. The coordination reaction of PDA can effectively control the release of strontium ions and avoid the negative effects caused by the high strontium concentration. Our in vitro experiments showed that the composite microspheres had good biocompatibility and that the PDA coating promotes cell adhesion. The slow release of strontium ions can effectively promote mesenchymal stem cells osteogenic differentiation and the vascularization of endothelial cells. In addition, we injected composite microspheres into cranial defects of rats to evaluate osseointegration in vivo. The results showed that nHA/CS@PDA-Sr could effectively promote bone regeneration in the defect area. This study demonstrates that composite microspheres stimulate bone repair providing a promising way for bone-defect regeneration.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
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IF 8.2
Zhiyuan Meng,Zhonglong Wang,Yueyin Liang,Guocheng Zhou,Xinyan Li,Xu Xu,Yiqin Yang,Shifa Wang
As one of the most abundant metal ions, Cu 2+ has turned into a great threat to human health and the natural environment due to its widely utilized in various industries. In this paper, a chitosan-based fluorescent probe CTS-NA-HY for detection and adsorption of Cu 2+ was rationally prepared. CTS-NA-HY exhibited a specific “turn off” fluorescence response to Cu 2+ and the fluorescence color changed from bright yellow to colorless. It possessed satisfactory detection performance to Cu 2+ including good selectivity and anti-interference, low detection limit (29 nM) and wide pH range (4–9). The detection mechanism was confirmed by Job's plot, X-ray photoelectron spectroscopy, FT-IR and 1 H NMR analysis. Additionally, the probe CTS-NA-HY was capacity of determining Cu 2+ in environmental water and soil samples. Besides, CTS-NA-HY -based hydrogel could also remove Cu 2+ in aqueous solution effectively, which the ability of adsorption was greatly improved compared with original chitosan hydrogel.
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CARBOHYDRATE POLYMERS
·
2022
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IF 10.723
Xue Zhang,Xueya Xu,Xiaoying Wang,Yajuan Lin,Yaling Zheng,Wen Xu,Jian Liu,Wei Xu
A glycyrrhetinic acid-modified carboxymethyl chitosan-thioketal-rhein (GCTR) conjugate was designed and synthesized for the in vivo delivery of celastrol (Cela). Cela was encapsulated into polymeric micelles (PMs) formed by GCTR conjugates self-assembly in water to form Cela/GCTR PMs with high drug loading capacity and small particle size. Cela/GCTR PMs had a sustained-release characteristic in the blood environment and a rapid-release feature in the tumor microenvironment. Cela/GCTR PMs had a significant proliferation inhibitory effect on HepG2 and BEL-7402 cells, but a negligible impact on L-02 cells at low concentrations. Cela/GCTR PMs possessed reactive oxygen species (ROS)-responsive properties in vitro and in cells, could improve the bioavailability of Cela, and exert remarkable hepatoma-targeting properties. Cela/GCTR PMs could also effectively inhibit tumor growth with no apparent damage to different organs. In summary, GCTR PMs with good ROS-responsive and hepatoma-targeting properties are expected to be possible delivery carriers for hydrophobic antineoplastic drugs for hepatocellular carcinoma therapy.
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NANO
·
2022
·
IF 1.438
Qian Sun,Jin Yang,Zulin Da,Yongsheng Yan
With the rapid development of the chemical industry, oil/ water emulsion separation is receiving global attention. It is particularly important to find a low-cost, convenient, economical and environmentally friendly method to prepare superhydrophilic/ underwater superoleophobic membranes for oil/ water emulsion separation. In this paper, discarded cigarette butts were used as raw materials, combined with chitosan to form a superhydrophilic/ underwater superoleophobic membrane through a phase inversion method. The performance of CA/CS membrane is controlled by adjusting the ratio of cellulose acetate and chitosan. The cigarette butts are washed in ethanol solution to remove impurities to obtain cellulose acetate, and then chitosan and polyvinylpyrrolidone (PVP) are added to build the micro–nano structure on the surface of the membrane. The prepared CA/CS composite membrane was superhydrophilic/ underwater superoleophobic. For the oil-in-water emulsion, the filtrate of the CA/CS membrane can hardly see the oil droplets through electron microscope. CA/CS membrane has a higher emulsion permeation flux (more than 340 L m − 2 h − 1 ) and a higher separation efficiency (more than 97.1%). In addition, the membrane has good reusability within 10 cycles. This method can reduce the harm of cigarette butts to nature and can also be used for oil–water separation. The method of turning discarded cigarette butts into treasure is worthy of social advocacy.
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JOURNAL OF APPLIED POLYMER SCIENCE
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2022
·Vol. 140
·期 8
·e53501
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IF 3.057
Chenxi Lou,Xinwen Liu,Cong Yang,Fenxia Ye,Qi Zhou
A one-step method was developed for synthesizing Ag nanoparticles exposed on the chitosan-covered polyamide 6 (CS-Ag@PA6) nanofibers by the in-situ reduction of silver nitrate during the electrospinning process. The bioderived CS was employed as a stabilizer and chelating carrier of Ag 0 /Ag + through its functional groups. CS was also used as a tractive agent to promote the surface migration of Ag atoms owing to its polyelectrolytic properties and immiscibility with PA6. Formic acid was used as the co-solvent for PA6 and CS and the reducing agent for Ag + . It was verified that the Ag nanoparticles (AgNPs) with 31.7% Ag 0 were embedded inside the pure PA6 nanofibers. However, the incorporation of CS achieved the AgNPs with an improved reduction efficiency (i.e., 67.5% of Ag 0 ). In particular, the CS nanolayers were covered on the PA6 nanofiber skeleton, where the AgNPs migrated on the surface of the CS nanolayers. This surface migration of CS and AgNP S endowed a significantly enhanced catalytic activity to the reduction of 4-nitrophenol, where the reduction rate constant ( k ) and turnover frequency (TOF) were an order of magnitude higher than that of the CS-free nanofibers.
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International Journal of Nanomedicine
·
2023
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IF 8
Jin Zhang,Chuan-Zhen Ye,Ze-Yu Liu,Qian Yang,Yong Ye
Background Bacterial resistance to antibiotics is a persistent and intractable problem. The sapogenin isolated from the seeds of Camellia oleifera can inhibit antibiotic-resistant bacteria after structural modification.Purpose This study aims to improve sapogenin's antibacterial activity and avoid bacterial resistance based on nano-preparation with photo responsiveness.Methods The liposome shell material of carboxymethyl chitosan-phosphatidyl ethanolamine (CMC-PE) was prepared using amidation reaction, and photo-responsive cationic (PCC) liposomes containing Camellia sapogenin derivative (CSD) and photosensitizer pheophorbide-a were prepared by film dispersion method. Encapsulation efficiency, drug loading, zeta potential, particle size distribution, morphology and stability of the PCC liposomes were determined by HPLC, particle size analyzer, transmission electron microscopy (TEM) and fluorescence microscopy. Photo-responsive release of CSD in the PCC liposomes was determined by laser (0.5 mW/cm2) at 665 nm. Antibacterial activity of the PCC liposomes with or without irradiation was analyzed by MIC50, MBC, MBIC50, and bacterial morphology to evaluate the antibacterial effects on amoxicillin resistant Escherichia coli and Staphylococcus aureus.Results Size distribution, zeta potential, encapsulation efficiency and drug loading of the PCC liposomes were 189.23 ± 2.12 nm, 18.80 ± 1.57 mV, 83.52 ± 1.53% and 2.83 ± 0.05%, respectively. The PCC liposomes had higher storage stability and gastrointestinal stability, and no obvious hemolytic toxicity to rabbit red blood cells and no cytotoxicity after incubation with Hela cells. The photosensitizer pheophorbide-a was uniformly dispersed in the phospholipid layer of the PCC liposomes and increased the CSD release after irradiation. The PCC liposomes could bind to bacteria and impaired their morphology and structure, and had significant bactericidal effect on amoxicillin resistant E. coli and S. aureus.Conclusion The photo-responsive PCC liposomes are efficient antibacterial agents for avoidance of bacterial resistance against antibiotics.
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International Journal of Nanomedicine
·
2023
·
IF 8
Wei Xu,Honglan Wang,Lihui Dong,Pan Zhang,Yan Mu,Xueyan Cui,Jianping Zhou,Meirong Huo,Tingjie Yin
Introduction: Herein, a hyaluronic acid (HA)-coated redox-sensitive chitosan-based nanoparticle, HA(HECS-ss-OA)/GA, was successfully developed for tumor-specific intracellular rapid delivery of gambogic acid (GA).Materials and methods: The redox-sensitive polymer, HECS-ss-OA, was prepared through a well-controlled synthesis procedure with a satisfactory reproducibility and stable resulted surface properties of the assembled cationic micelles. GA was solubilized into the inner core of HECS-ss-OA micelles, while HA was employed to coat outside HECS-ss-OA/GA for CD44-mediated active targeting along with protection from cation-associated in vivo defects. The desirable redox-sensitivity of HA(HECS-ss-OA)/GA was demonstrated by morphology and particle size changes alongside in vitro drug release of nanoparticles in different simulated reducing environments.Results: The results of flow cytometry and confocal microscopy confirmed the HA-receptor mediated cellular uptake and burst drug release in highly reducing cytosol of HA(HECS-ss-OA)/GA. Consequently, HA(HECS-ss-OA)/GA showed the highest apoptosis induction and cytotoxicity over the non-sensitive (HA(HECS-cc-OA)/GA) and HA un-coated (HECS-ss-OA/GA) controls against A549 NSCLC model both in vitro and in vivo. Furthermore, a diminished systemic cytotoxicity was observed in HA(HECS-ss-OA)/GA treated mice compared with those treated by HA un-coated cationic ones and GA solution.
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Membranes
·
2022
·Vol. 13
·期 1
·36
·
IF 4.562
Yunwu Yu,Chunyang Xie,Yan Wu,Peng Liu,Ye Wan,Xiaowei Sun,Lihua Wang,Yinan Zhang
In this study, a novel composite membrane was developed by casting the mixed aqueous solution of chitosan (CS) and polyvinyl alcohol (PVA) on a glass fiber microporous membrane. The polymeric coating of a composite membrane containing amino groups and hydroxyl groups has a favorable CO2affinity and provides an enhanced CO2transport mechanism, thereby improving the permeance and selectivity of CO2. A series of tests for the composite membranes were taken to characterize the chemical structure, morphology, strength, and gas separation properties. ATR-FTIR spectra showed that the chemical structure and functional group of the polymer coating had no obvious change after the heat treatment under 180 °C, while SEM results showed that the composite membranes had a dense surface. The gas permeance and selectivity of the composite membrane were tested using single gases. The results showed that the addition of chitosan can increase the CO2permeance which could reach 233 GPU. After a wetting treatment, the CO2permeance (454 GPU) and gas selectivity (17.71) were higher than that of dry membranes because moisture promotes the composite membrane transmission. After a heat treatment, the permeance of N2decreased more significantly than that of CO2, which led to an increase in CO2/N2selectivity (10.0).Keywords:carbon dioxide;polyvinyl alcohol;chitosan;glass fiber microporous membrane;gas separation
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ACS Applied Materials & Interfaces
·
2023
·
IF 9.5
Baokang Wang,Jupen Liu,Ping Zhang,Hongqiu Wei,You Yu
Natural and biocompatible chitosan has demonstrated wide applications. However, rapidly fabricating high-performance chitosan hydrogels in one-step controllable processes is still a challenge for some advanced applications. Here, we report a trifunctional microgel-mediated photochemical (TMMP) strategy to achieve the fabrication of printable tough chitosan-based hydrogels (PTCHs) in seconds. Such microgels help the slow release of persulfate anions and their uniform dispersion in an aqueous solution of cationic chitosan. The released persulfates are available for preparing multiple networks of phenolic coupling of modified chitosan and radical polymerization of Pluronic F127 via orthogonal tris(bipyridine)ruthenium(II)-based photochemistry, respectively. Trifunctional microgels have reversible Ca2+-crosslinked networks that further improve the hydrogels’ mechanical properties and toughness. The maximum stress and toughness increase by >20 folds compared to the chitosan and F127 hydrogels with single network structures. Moreover, these microgels enable the precursor to have a good shearing-thinning property and benefit the controllable preparation of PTCHs in a short time, as low as ∼4 s under visible light irradiation. It, therefore, is compatible with standard printing techniques to make complex structures. Strain sensors based on structured PTCHs have stable mechanical and responsive properties in the water, which are applied for real-time underwater communications (<0.4 s). It is anticipated that this one-step TMMP strategy opens new horizons for designing advanced chitosan hydrogels.
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CARBOHYDRATE POLYMERS
·
2022
·
IF 10.723
Yue Zhu,Di Qin,Jinfeng Liu,Guangsheng Wu,Haonan Wang,Feifei Wu,Yixuan Liu,Ya Liu,Xiaojie Cheng,Xiguang Chen
Hydrogel, as three-dimensional (3D) cell culture scaffold, is an effective strategy for tissue and organ regeneration due to their good biocompatibility, biodegradability and resemblance to body microenvironments in vivo. However, the inherent weak mechanical properties and strong shrinkage of hydrogels during cell culture hinder its application in clinical. In this study, a two-component thermo/photo dual-sensitive hydrogel (M/C) was prepared from methacrylated hydroxybutyl chitosan (MHBC) and chitin whisker (CHW) via physical and chemical cross-linking methods. M/C hydrogel showed a special internal structure with lamellar arrangement. The rheological properties of the hydrogels could be regulated with the change of M/C ratio. It is worth emphasizing that the mechanical properties, shrinkage resistance and cellular capacitances of the M/C hydrogel were improved with the addition of CHW. Moreover, the M/C hydrogel not only exhibited excellent degradability and antibacterial properties , but also significantly promoted the adhesion and proliferation of MC3T3-E1 cells in vitro. Therefore, the M/C hydrogel showed a wide application potential in tissue regeneration as a 3D cell culture scaffold.
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JOURNAL OF MOLECULAR LIQUIDS
·
2022
·
IF 6.633
Chaojian Zhang,Tianrui Yang,Junsheng Liu,Qianqian Duan,Jiahui Song,Youjun Yin,Hansong Wang
Novel poly(ethylene imine) (PEI) modified chitosan-based hybrid membranes (PMCHMs) were created via a soft chemistry method, in which unmodified chitosan (CS) firstly reacted with PEI to produce a CS-PEI precursor. Later, such CS-PEI precursor was blended with silicone to create the membrane solution of PMCHMs. Finally, PMCHMs could be obtained via phase-inversion process. Multi-component sorption of Pb 2+ , Cu 2+ and Ni 2+ on PMCHMs in metal mixed solutions, such as, Pb 2+ &Cu 2+ and Pb 2+ &Ni 2+ binary, Pb 2+ , Cu 2+ and Ni 2+ ternary, as well as Pb 2+ , Cu 2+ , Ni 2+ , Cd 2+ and Cr 3+ quintuple mixed solutions, was inspected. The calculation of effective diffusion coefficients, distribution coefficients and selectivity coefficients showed that Pb 2+ sorption on PMCHMs had excellent selectivity in these mixed multi-metal solutions. While, the calculation of activation energy proved that Pb 2+ , Cu 2+ and Ni 2+ sorption on PMCHMs was a physic-sorption process Also, mechanism insight reveals that Pb 2+ sorption on PMCHMs could be depicted via Lagergren pseudo-2nd-order kinetic model and Langmuir or Freundlich isotherm equation. Exclusively, intra-particle diffusion process was discussed in detail. Desorption testing verified that HCl is a good desorbent for metals sorption on PMCHMs and the cycle time of membranes can arrive at 6 times. These results demonstrate that PMCHMs can be used to preferentially eradicate Pb 2+ from metal mixture solutions, showing prospective applications in the treatment of metal mixture wastewater from waste vehicle power-battery and other various metal-bearing polluted water.
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Sustainable Materials and Technologies
·
2023
·
IF 9.6
Jiafu Shen,Yu Dai,Fan Xia,Xiaojin Zhang
Due to high security and versatility, hydrogels have attracted great interest in sustainable applications such as wastewater treatment and energy storage. However, the poor adsorption capacity as an adsorbent and the low conductivity as an electrolyte seriously restrict the sustainable application of hydrogels. Here, we prepare nanocomposite hydrogels (PAAm/CS-EDTA/GO) by mixing ethylene diamine tetraacetic acid (EDTA)-modified chitosan (CS) and graphene oxide (GO) in crosslinking polymerization of acrylamide (AAm). Nanocomposite gives PAAm/CS-EDTA/GO hydrogel excellent mechanical properties (e.g., fracture energy of ∼1208 kJ∙m −3 ). EDTA, CS and GO support rich and special adsorption sites for the removal of metal ions and dyes. The addition of lithium chloride (LiCl) makes PAAm/CS-EDTA/GO/Li hydrogel have anti-freezing and anti-dehydration properties. Interestingly, the synergy of CS-EDTA and GO increases the conductivity of the hydrogel. The supercapacitor assembled by PAAm/CS-EDTA/GO/Li hydrogel electrolyte shows high capacitance and temperature tolerance. Our work provides a route for the rational development of adsorption materials and energy storage devices for sustainable applications.
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