HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK
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2020
·Vol. 42
·期 9
·2287-2297
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IF 2.538
Li-Bo Dai,Qi Yu,Shui-Hong Zhou,Yang-Yang Bao,Jiang-Tao Zhong,Li-Fang Shen,Zhong-Jie Lu,Jun Fan,Ya-Ping Huang
Background This study is to explore the role of curcumin and GLUT-1 antisense oligodeoxynucleotides (AS-ODN) on autophagy modulation-initiated radiosensitivity. Methods BALB/c mice were employed to establish xenograft model using Tu212 cell. The expression of autophagy- and apoptosis-related proteins was determined by WB. Autophagosome was observed under transmission electron microscope. Apoptosis of tumor tissue were detected by TUNEL staining. Results Combinations of curcumin and GLUT-1 AS-ODN with 10 Gy inhibited the tumor growth by inducing apoptosis of laryngeal cancer cells followed with the enhancement of autophagy. 3-MA also had a promotion effect on irradiation-mediated growth inhibition possibly by depressing PI3K and on curcumin/GLUT-1 AS-ODN-mediated growth inhibition potentially by regulating autophagic events. Of note, a de-escalation of radiotherapy dose (5 Gy) along with curcumin, GLUT-1 AS-ODN or 3-MA produced a stronger effect than high dosage of radiotherapy (10 Gy) alone. Conclusions Curcumin and GLUT-1 AS-ODN improve the radiosensitivity of laryngeal carcinoma through regulating autophagy and inducing apoptosis.
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Journal of Materiomics
·
2020
·
IF 5.797
Gang Liu,Jia Dong,Leiyang Zhang,Yan Yan,Ruiyi Jing,Li Jin
In the present work, the nature of phase evolution of (1− x )(Na 0.5 Bi 0.5 )TiO 3 - x SrTiO 3 (NBT- x ST) solid solutions with x of 0–0.6 is revealed by characterizing the dielectric and ferroelectric properties. Two unique dielectric anomalies associated with high-temperature nanoregions (PNRs) in the ergodic relaxor (ER) state and low-temperature PNRs in the nonergodic relaxor (NR) state are identified. Characteristic temperatures, including T B , T RT∗ , T m , T d and T T ∗ , are determined in fresh and poled states on the basis of the characteristics of the evolution of these two dielectric anomalies. The whole evolution of the transition from the NR state to the ER state is reflected by the temperature-dependent polarization versus electric field ( P - E ) hysteresis loops, i.e., from the square loops, via the double-like loops, to the slim loops. The characteristic temperatures, including T P-N , T N-R and T R-dis , are determined by the characteristics of the evolution of P - E loops. Accordingly, a phase diagram of NBT- x ST was constructed according to these characteristic temperatures. Most importantly, the relationship between polarization responses and heterogeneous polar phase coexistence has been established and a schematic diagram is given. This work will help to understand the phase evolution and its impact on the macroscopic properties of NBT and the associated NBT-based solid solutions.
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ENERGY & FUELS
·
2020
·
IF 3.421
Ruihan Luo,Xiuge Zhao,Honghui Gong,Wei Qian,Difan Li,Manyu Chen,Kai Cui,Jiajia Wang,Zhenshan Hou
Hydrogenolysis of glycerol to propanols is highly promising for the sustainable development of biomass refining. In this work, modified amorphous zirconium phosphate (ZrP)-supported Pt catalysts were developed and characterized thoroughly by X-ray diffraction, temperature-programmed desorption of ammonia, Fourier transform infrared spectra of adsorbed pyridine, temperature-programmed reduction, high-resolution transmission electron microscopy, X-ray photoelectron spectroscopy, and Brunauer–Emmett–Teller surface area. The as-obtained catalysts were employed for selective glycerol hydrogenolysis to 1-propanol (1-PO) in a fixed-bed reactor. Owing to the incorporation of WOx, both the total acidity and the dispersion of Pt nanoparticles were enhanced significantly. The Pt/7WOx-ZrP catalyst could afford 81% yield of 1-PO at full conversion of glycerol in a long-term catalytic stability test (at least 70 h reaction time) and was regenerated by calcination in air. The results of the catalytic activity test and catalyst characterization indicated that the acid sites of the catalysts promoted the selective dehydration of glycerol into acrolein, whereas the Pt sites facilitated H2 dissociation and a sequential hydrogenation of acrolein into 1-PO.
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MOLECULES
·
2020
·Vol. 25
·期 11
·2475
·
IF 3.267
Yanliang Yang,Dexi Yang,Chi Zhang,Min Zheng,Ying Duan
The development of a simple and durable catalytic system for the production of chemicals from a high concentration of a substrate is important for biomass conversion. In this manuscript, 5-hydroxymethylfurfural (HMF) was converted to 1-hydroxy-2,5-hexanedione (HHD) using the combination of commercial Pd/C and acetic acid (AcOH) in water. The influence of temperature, H2pressure, reaction time, catalyst amount and the concentration of AcOH and HMF on this transformation was investigated. A 68% yield of HHD was able to be obtained from HMF at a 13.6 wt% aqueous solution with a 98% conversion of HMF. The resinification of intermediates on the catalyst was characterized to be the main reason for the deactivation of Pd/C. The reusability of the used Pd/C was studied to find that most of the activity could be recovered by being washed in hot tetrahydrofuran.Keywords:HMF;1-hydroxy-2,5-hexanedione;acetic acid;Pd/C;high concentration
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APPLIED CATALYSIS A-GENERAL
·
2020
·
IF 5.006
Pengjia Yang,Wentao Zhong,Baiyu Jiang,Biao Zhang,Zhisheng Fu,Zhiqiang Fan
Propagation chains with 1,2 and 2,1 last-inserted monomer unit in 1-hexene polymerization with MgCl 2 -supported Ziegler-Natta catalysts were quench-labeled by thiophene-2-carbonyl chloride, and the two kinds of 2-thenoyl-labeled chain ends were determined by 1 H NMR analysis. The number of labeled chains was noticeably smaller than the number of dead chains formed by chain transfer with alkylaluminum cocatalyst, and grew with time more slowly than the latter, showing that side reactions between the quencher and Al-polymeryl moieties are negligible. The active sites with 2,1 last-inserted monomer were halted in dormant state, which lead to their gradual accumulation in the 30 s polymerization process. The catalyst containing phthalate type internal donor has higher percentage of dormant sites as compared with the catalyst containing diol ester. The combination of acyl chloride quench-labeling and 1 H NMR analysis forms a reliable and easily accessible method of tracing and characterizing active sites in transition metal catalyzed olefin polymerization.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2021
·
IF 7.312
Shujuan Yang,Rong Tang,Yanan Dai,Tianhao Wang,Zhinong Zeng,Liping Zhang
To expand the application of cellulose acetate (CA) membranes in wastewater treatment, the excellent ultrafiltration performance and anti-fouling properties should be achieved. Firstly, HKUST-1@ lignocellulose nanofibrils (LCNFs) was fabricated through green and in-situ strategy successfully, which combine the characteristics of the HKUST-1 and LCNFs. Then, the blended ultrafiltration membranes with different loading of HKUST-1@LCNFs (or HKUST-1, LCNFs) were prepared by the process of phase separation, which exhibit improved pore structure, hydrophilicity, and rejection rate. Excitedly, the highest pure water permeability of 207.32 L·m −2 ·h −1 and the flux recovery ratio (FRR) of 90.56% were obtained with M6 membrane (0.55 wt% HKUST-1@LCNFs), which was better than that of M2 (HKUST-1) and M3 (LCNFs). It is confirmed that HKUST-1 could inhibit the accumulation of LCNFs, making it taking advantages of its hydrophilic groups. Moreover, composite membranes have attractive antibacterial properties, and >90% of E. coli were killed by M7. This work demonstrated that CA membranes blended with HKUST-1@LCNFs possesses great potential in the wastewater treatment process.
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ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Xiaolun Peng,Yijia Wang,Wei Wen,Miao-Miao Chen,Xiuhua Zhang,Shengfu Wang
Programmed cell death protein 1 (PD-1) is one of the coinhibitory checkpoints upon T cell activation, the abnormal expression of which severely threatens host immune modulatation for chronic infection. Thus, fast and sensitive monitoring of PD-1 is of vital importance for early diagnosis and cancer treatment. The current detection methods largely based on enzyme-linked immunosorbent assay (ELISA) require time-consuming incubation and complicated washing steps. Herein, we designed a simple and portable nanofiber paper (NFP)-based fluorescence “off-on” immunosensor for PD-1 rapid determination. Molybdenum disulfide (MoS2) nanosheets modified NFP (MoS2–NFP) was employed for adsorbing and immobilizing CdSe/ZnS quantum dots-antibody (QDs-Ab) complex to construct a ready-to-use fluorescent immunosensor. The fluorescent signal of QDs-Ab was initially quenched by MoS2 under the Förster resonance energy transfer (FRET) effect. When the PD-1 target was specifically captured onto NFP by immunization, the QDs-Ab-PD-1 complex was promptly desorbed from the MoS2–NFP surface, resulting in FRET impediment and fluorescence recovery. As an alternative quenching agent, graphene oxide (GO) served as a contrast to investigate NFP-based sensing performance. Owing to superior quenching and desorption efficiency, the MoS2–NFP-based fluorescence immunosensor exhibited nearly 2-fold lower detection limit (85.5 pg/mL) than GO–NFP-based sensor (151 pg/mL) for PD-1 monitoring. Excellent selectivity and satisfactory recovery in PD-1 mouse cell culture supernatant samples were confirmed as well. In addition, the comparable detectability of the MoS2–NFP-based immunosensor was accurately evaluated by a standard PD-1 mouse ELISA kit. This study displayed a simple, rapid, low-cost, and portable point-of-care PD-1 assay, indicating its broad application prospect toward clinical diagnoses.
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MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 8
·1-11
·
IF 5.833
Feng Jun,Lu Hao,Yang Yu,Huang Wenyi,Cheng Hao,Kong Hongxing,Li
Lijun
By in situ synthesis of gold nanoparticles (AuNPs) within the acid-etched (AE) MIL-101 (Cr) framework, AE-MIL-101 (Cr) nanocomposites embedded with AuNPs (AuNP/AE-MIL-101 (Cr)) were prepared as surface-enhanced Raman scattering (SERS) substrate. AuNPs are uniformly distributed and stabilized inside the metal-organic framework (MOF), thus forming more SERS hotspots. The SERS performance of AuNP/AE-MIL-101 (Cr) was evaluated using 4-mercaptophenylboronic acid (4-MPBA), 4-mercaptobenzoic acid (4-MBA), benzidine, and rhodamine 6G (R6G). The SERS substrate displays satisfying stability with very low background signal. When benzidine is used as the Raman reporter, the limit of detection (LOD) can reach 6.7 × 10 −13 mol·L −1 , and the relative standard deviation (RSD) of the intra- and inter-batch repetitive tests is less than 5.2%. On this basis, we developed a method for the detection of human carboxylesterase 1 (hCE 1) in human serum using AuNP/AE-MIL-101 (Cr) nanocomposite as SERS substrate and enzyme-linked immunosorbent assay (ELISA) colorimetric substrate as SERS marker. This method was used to determine hCE 1 in clinical serum samples without complicated sample pretreatment, and the detection results were consistent with the data determined by ELISA. In the concentration range 0.1–120 ng·mL −1 , the SERS signal intensity of benzidine at 1609 cm −1 gradually decreases with the increase of hCE 1 concentration (R 2 = 0.9948). The average recoveries of hCE 1 in human serum are in the range 84 to 108%, with RSDs lower than 7.7%. Graphical abstract
By using AuNP/acid etching-MIL-101(Cr) metal organic framework (MOF) as SERS substrate and enzyme-linked immunosorbent assay (ELISA) colorimetric substrate as the SERS marker, a rapid and sensitive method for the determination of human carboxylesterase 1 (hCE1) in human serum samples has been developed.
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JOURNAL OF BIOLOGICAL CHEMISTRY
·
2019
·Vol. 294
·期 52
·20164-20176
·
IF 4.106
Shijiao Tian,Wenming Jia,Mei Lu,Juan Zhao,Xiulian Sun
Insulin resistance in the brain is a pathological mechanism that is shared between Alzheimer's disease (AD) and type 2 diabetes mellitus (T2DM). Although aberrant expression and phosphorylation of insulin receptor substrate 1 (IRS-1) contribute to insulin resistance, the underlying mechanism remains elusive. In this study, we used several approaches, including adeno-associated virus-based protein overexpression, immunoblotting, immunoprecipitation, immunohistochemistry, and in situ proximal ligation assays, to investigate the function of dual-specificity tyrosine phosphorylation-regulated kinase 1A (DYRK1A) in IRS-1 regulation and the downstream insulin signaling in neurons. We found that DYRK1A overexpression up-regulated IRS-1 expression by slowing turnover of the IRS-1 protein. We further observed that DYRK1A directly interacted with IRS-1 and phosphorylated IRS-1's multiple serine residues. Of note, DYRK1A and IRS-1 were coordinately up-regulated in the prefrontal cortex of db/db mice brain. Furthermore, DYRK1A overexpression ameliorated chronic high insulin-induced insulin resistance in SH-SY5Y cells as well as in primary rat neurons. These findings suggest that DYRK1A protects against insulin resistance in the brain by elevating IRS-1 expression.
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Green Energy & Environment
·
2019
·
IF 0
Yu-Mei Liu,Yan Zhou,Wen-Qiang Gong,Zhang-Min Li,Chao-Li Wang,Duan-Jian Tao
The catalysis of ionic liquids (ILs) in the traditional stirred reactor suffers from insufficient mass and heat transfer, which always needs a long reaction time and results in a low reaction rate. In this work, highly efficient synthesis of 1-methoxy-2-propanol via the alcoholysis reaction of propylene oxide (PO) with methanol was proposed and achieved by the combination of micro-tubular circulating reactor with the IL [N 4444 ][Buty] catalyst. Compared with the stirred reactor, the rate of alcoholysis reaction in a micro-tubular circulating reactor was found to be significantly improved. The reaction time was remarkably shortened to 20 min from 180 min as well as the yield of 1-methoxy-2-propanol reached 92%. Moreover, the kinetic study further demonstrated that the main reaction rate to 1-methoxy-2-propanol (K 1 ) was about 20 times larger than the side reaction rate to byproduct 2-methoxy-1-propanol (K 2 ) in the temperature range of 363–383 K. Such combination of micro-tubular circulating reactor with IL catalysts is believed to be a class of effective process intensification technique for highly efficient synthesis of 1-methoxy-2-propanol.
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NANOTECHNOLOGY
·
2019
·Vol. 30
·期 48
·485706
·
IF 3.399
Feng Long,Wei Li,Wanling Chen,Danni Liu,Yue Chen,Rui Zhou,Penghui Li
In this work, a novel label-free electrochemical biosensor is developed for the detection of galectin-1 (Gal-1) based on gold nanoparticle (AuNP) loaded octahedral Cu2O (Cu2O@Au) nanocomposites. The AuNPs on the surface of the Cu2O nanocrystals not only enhance the electrochemical performance, but also serve as the binding sites for the lactose ligand which can specifically bind with Gal-1. The Cu2O@Au nanocomposites provide the synergic effect of electrochemical signal amplification and lactose–galectin reaction as the recognition strategy. Under optimal conditions, the proposed biosensor exhibits a variation of electrochemical responses to different concentrations of Gal-1 ranging from 0.1 pg ml−1 to 10 ng ml−1. This work presents an alternative electrochemical biosensor for the detection of tumor biomarkers based on a simple and economical lactose ligand incorporated Cu2O@Au biosensor platform.
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Journal of Energy Storage
·
2019
·
IF 3.517
Shengqin Gong,Xiaomin Cheng,Yuanyuan Li,Dawei Shi,Xiuli Wang,Hao Zhong
The porous Al 2 O 3 ceramic foam was composited with expanded graphite (EG) by calcination method to obtain Al 2 O 3 @EG composite with hierarchical pores. The specific surface area of Al 2 O 3 @EG composite was 2.3 times that of EG , which can better enhance the thermal conductivity and heat transfer performance of phase change materials (PCMs). The stable composite PCMs with high thermal conductivity and energy storage density were prepared via vacuum adsorbing 1-octadecanol (OD) in Al 2 O 3 @EG. The thermal conductivity of the modified OD containing 10 wt% Al 2 O 3 @EG was 4.5 times that of pure OD, while maintaining a high phase change enthalpy, approximately 200 kJ/kg. The results of SEM and EDS indicated that Al 2 O 3 foam particles dispersed equably on the surface of EG without obvious agglomeration. The XRD and FTIR results revealed that OD, Al 2 O 3 and EG were physically combined. The prepared OD/Al 2 O 3 @EG composite PCMs with high thermal conductivity and large energy storage density can be promising for low temperature heat storage, such as industrial heat recovery system, comfort applications in buildings and textiles, solar energy system, etc. This study provides an innovative idea for the application of porous ceramic foams in the field of solar energy storage PCMs modification.
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