JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2014
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IF 2.694
Yunfang Li,Yu Chen,Chuying Xiao,Dan Chen,Yuxiu Xiao,Zhinan Mei
α-Amylase inhibitors play an important role in management of diabetes and obesity. In order to rapidly discover potent α-amylase inhibitors from medicinal plants, a ligands-screening method based on enzyme-immobilized magnetic nanoparticles integrated with HPLC was developed. Amine-terminated magnetic nanoparticles were prepared for the immobilization of α-amylase. Based on the affinity theory, the α-amylase-coated magnetic nanoparticles were employed to fish out the ligands from the extracts of Garcinia xanthochymus , and the elutes were examined by HPLC. As a result, three ligands were screened out. Isolation and identification were carried out subsequently. By analyzing the UV, MS and NMR spectra, they were identified as three biflavonoids including GB2a glucoside (2), GB2a (3) and fukugetin (4). The IC 50 values of the three compounds were also determined. The results suggest the proposed approach is efficient and accurate, and has great potential in rapid discovery of drug candidates from medical plants.
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Materials Science & Engineering C-Materials for Biological Applications
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2015
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IF 3.088
Xiaoju Wang,Cuiping Yang,Chenhong Wang,Leijia Guo,Tianhong Zhang,Zhenqing Zhang,Husheng Yan,Keliang Liu
Although PEG remains the gold standard for stealth functionalization in drug delivery field up to date, complete inhibition of protein corona formation on PEG-coated nanoparticles remains a challenge. To improve the stealth property of PEG, herein an α-glutamyl group was conjugated to the end of PEG and polymeric micelles with α-glutamyl-terminated PEG shells were prepared. After incubation with bovine serum albumin or in fetal calf serum, the size of the micelles changed slightly, while the size of the micelles of similar diblock copolymer but without α-glutamyl group increased markedly. These results indicated that the micelles with α-glutamyl-terminated PEG shells showed low non-specific protein adsorption. In vivo blood clearance kinetics assay showed that the micelles with α-glutamyl-terminated PEG shells exhibited a longer in vivo blood circulation time compared with similar micelles but without α-glutamyl groups. The better stealth property of the micelles with α-glutamyl-terminated PEG shells was presumably attributed to the zwitterionic property of the α-glutamyl groups.
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HYDROMETALLURGY
·
2016
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IF 2.29
Shengting Kuang,Zhifeng Zhang,Yanling Li,Guolong Wu,Haiqin Wei,Wuping Liao
A novel extractant, di (2-ethylhexyl) (2-((2-ethylhexyl) amino) propan-2-yl) phosphonate (DEHAPP, abbreviated as B ), was synthesized for the selective extraction of Ce(IV) in sulfate medium. The effect of contact time, the H 2 SO 4 concentration, the DEHAPP concentration and temperature on the Ce(IV) extraction were studied systematically. DEHAPP exhibits a good extraction ability towards Ce(IV) when compared with other neutral organophosphorus extractants such as Cyanex 923, Cyanex 925 and DEHEHP. The extraction equilibrium constants and thermodynamic parameters (Δ G , Δ H and Δ S ) were calculated. The extraction reaction of Ce(IV) is an endothermic process. The extracted complexes were determined to be H 2 SO 4 · B ( org ) and Ce(HSO 4 ) 2 SO 4 ·2 B ( org ) . A extraction process to selectively recover Ce(IV) from bastnaesite leaching was proposed, by which the purity of cerium product reached 99.5% with a yield of 90.7%.
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MOLECULES
·
2024
·Vol. 29
·期 5
·1181
·
IF 4.2
Minghe Wang,Zhouyuji Liao,Dongting Zhangsun,Yong Wu,Sulan Luo
Antimicrobial peptides (AMPs), acknowledged as host defense peptides, constitute a category of predominant cationic peptides prevalent in diverse life forms. This study explored the antibacterial activity of α-conotoxin RgIA, and to enhance its stability and efficacy, D-amino acid substitution was employed, resulting in the synthesis of nine RgIA mutant analogs. Results revealed that several modified RgIA mutants displayed inhibitory efficacy against various pathogenic bacteria and fungi, includingCandida tropicalisandEscherichia coli. Mechanistic investigations elucidated that these polypeptides achieved antibacterial effects through the disruption of bacterial cell membranes. The study further assessed the designed peptides’ hemolytic activity, cytotoxicity, and safety. Mutants with antibacterial activity exhibited lower hemolytic activity and cytotoxicity, with Pep 8 demonstrating favorable safety in mice. RgIA mutants incorporating D-amino acids exhibited notable stability and adaptability, sustaining antibacterial properties across diverse environmental conditions. This research underscores the potential of the peptide to advance innovative oral antibiotics, offering a novel approach to address bacterial infections.
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Translational Research
·
2024
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IF 6.4
Shan-Shan Li,Qiao-Juan Liu,Jia-Xin Bao,Meng-ting Lu,Bing-Quan Deng,Wen-Wen Li,Chang-Chun Cao
Peptide drug discovery for the treatment of chronic kidney disease (CKD) has attracted much attention in recent years due to the urge to find novel drugs and mechanisms to delay the progression of the disease. In this study, we identified a novel short peptide (named YR-7, primary sequence ‘YEVEDYR’) from the natural Fibroin protein, and demonstrated that it significantly alleviated pathological renal changes in ADR-induced nephropathy. PANX1 was identified as the most notably upregulated component by RNA-sequencing. Further analysis showed that YR-7 alleviated the accumulation of lipid droplets via regulation of the lipid metabolism-related proteins PPAR α and PANK1. Using chemical proteomics, fluorescence polarization, microscale thermophoresis, surface plasmon resonance, and molecular docking, YR-7 was proven to directly bind to β-barrel domains of TGM2 protein to inhibit lipid accumulation. TGM2 knockdown in vivo increased the protein levels of PPAR α and PANK1 while decreased the levels of fibrotic-related proteins to alleviate nephropathy. In vitro , overexpression TGM2 reversed the protective effects of YR-7. Co-immunoprecipitation indicated that TGM2 interacted with PANX1 to promote lipid deposition, and pharmacological inhibition or knockdown of PANX1 decreased the levels of PPAR α and PANK1 induced by ADR. Taken together, our findings revealed that TGM2-PANX1 interaction in promoting lipid deposition may be a new signaling in promoting ADR-induced nephropathy. And a novel natural peptide could ameliorate renal fibrosis through TGM2-PANX1-PPAR α/PANK1 pathway, which highlight the potential of it in the treatment of CKD.
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ADVANCED FUNCTIONAL MATERIALS
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2024
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IF 18.5
Jiafeng Wang,Jinwei Xiu,Guanhaojie Zheng,Dong He,Han Gao,Zhenhua Chen,Zhaoning Li,Guocong Chen,Xusheng Zhang,Guoqiang Ma,Peter Raymond Slater,Zhubing He
It's still a scientific issue to synthesize exotic amines-free and pure-phase FAPbI 3 film because most FAPbI 3 films are synthesized by adding MACl-like exotic amines salts in their precursor solution to reduce the phase transition barrier. In this work, high-quality and additive-free phase-pure α-FAPbI 3 films are successfully fabricated via the sustainable Alkane/Nanocrystals method. FAPbI 3 nanocrystals (NCs) are performed as the heterogeneous nuclei to promote the nucleation and crystallization of FAPbI 3 films. Cryogenic-TEM and in situ GIWAXS evidence the seed function and interesting phase evolution of FAPbI 3 NCs. The whole phase transition course of pure FAPbI 3 film is systematically studied. IR-AFM and ToF-SIMS reveal ligands capped on NCs are extruded to the film surface. PbAc-IPA solution treating the film surface can effectively wash off the surplus ligands at the surface and modulate the surface chemistry and energy levels. As a result, an average power conversion efficiency (PCE) of 21.45% is achieved for MA-free pure α-FAPbI 3 perovskite solar cells (PSCs), as well as the best light-soaking stability record of retaining 95% of the initial PCE after 800 h. This work paves a new way to fabricate MA-free pure α-FAPbI 3 PSCs.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Liangqiang Chen,Mengdi Hao,Wanqiu Huang,Shaoning Yu,Hao Shen,Fan Yang,Li Wang,Huabin Tu
Multiple enzymes induce biological cascade catalysis is essential in nature and industrial production. However, the shortcomings of enzymes, including unsatisfactory stability, reusability, and sensitivity in harsh microenvironment, have restricted their broader use. Here, we report a facile method for fabricating a cascade system by combining the benefits of immobilized enzymes and biomimetic catalysis based on magnetic metal–organic framework nanoflowers (mMOFNFs). mMOFNFs prepared through the layered double hydroxide-derived strategy exhibited remarkable peroxidase-like activity and accessible amino interface, enabling it to serve not only as a reliable carrier for α-glucosidase and glucose oxidase fixation, but also as a nanozyme participating in cascade. On this basis, a colorimetric biosensor of excellent sensitivity and selectivity for α-amylase detection was constructed with a wide range (2–225 U L −1 ), low detection limit (2.48 U L −1 ), and rapid operation (30 min). This work provides a versatile strategy for establishing multi-enzyme cascade systems and rapid analysis of α-amylase.
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Foods
·
2024
·Vol. 13
·期 24
·4183
·
IF 4.7
Rourou Wen,Xianghua Chai,Pingping Wang,Kegang Wu,Xuejuan Duan,Jiasi Chen,Tong Zhang,Liya Zeng
Tea polyphenols have been reported to decrease the rate of starch hydrolysis by inhibiting α-glucosidase. However, the effect of the tea harvesting season and the structure of catechin monomers on the inhibitory activity of α-glucosidase is not understood. In this study, the inhibitory effect and underlying mechanism of four seasons of Dancong tea against α-glucosidase were investigated by in vivo and in vitro experiments, multi-spectroscope and molecular dynamic. The Dancong tea harvested in spring and winter showed a stronger inhibitory effect on α-glucosidase due to a higher content of catechin, especially EGCG ((-)-epigallocatechin-3-gallate). The results of in vivo and in vitro experiments showed that EGCG and ECG ((-)-epicatechin-3-gallate) with a higher content of gallate and hydroxyl groups exhibited a stronger inhibitory effect on starch hydrolysis, rise of postprandial blood glucose and activities of α-glucosidase compared to EGC ((-)-epigallocatechin) and EC ((-)-epicatechin). These gallate and hydroxy groups were more effective in interacting with the amino acid residues in the active site of α-glucosidase, leading to structural changes in the enzyme. Certainly, the inhibitory effect of Dancong tea on α-glucosidase explains one of the mechanisms by which it helps alleviate diabetes; the other hypoglycaemic mechanisms of Dancong tea will be further explored.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Keheng Zhu,Zhenhua Zhu,Shanshan Xu,Cheng Zhao,Tianjun Ni
Trimethylamine (TMA) is an organic amine with strong pungent smell which is an indicator gas for evaluating fish freshness according to the international standard. In this work, as-synthesize α-Fe 2 O 3 solid nanocubes (NCs), α-Fe 2 O 3 nucleoshell NCs and α-Fe 2 O 3 hollow NCs were used as sensing material to develop an outstanding TMA gas sensor. The response of the α-Fe 2 O 3 hollow NCs sensors towards 20 ppm TMA at 230 ℃ was 6.3. Meanwhile, these sensors showed exceptional response/recovery time, low limit of detection, great selectivity, and outstanding linear relationship. Furthermore, the analysis of gases released during the decomposition of Carassius auratus (0–10 days) was conducted, which demonstrated the assessment of TMA by α-Fe 2 O 3 hollow NCs sensor can evaluate the freshness of Carassius auratus . Such a novel sensor signifies the outstanding application potential in efficient gas-sensing properties of TMA, which will make the tremendous contribution for Carassius auratus product evaluation in the future.
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FOOD HYDROCOLLOIDS
·
2025
·
IF 12.4
Caiyun Liu,Lulu Wu,Zengshuai Zhang,Ziying Li,Mario Prejanò,Tiziana Marino,Yongsheng Tao,Yunkui Li
This study was aimed to explore the effect of α-cyclodextrin (α-CD) on color quality and stability of red wines, and to dissect the mechanism of copigmentation at molecular level. Firstly, α-CD was added to real wine before fermentation and aging dynamics were monitored. This study found that α-CD enhanced visual quality by strengthening anthocyanins maceration during fermentation, yet α-CD improved color stability by promoting formation of color-stable polymeric anthocyanins during aging. Secondly, the thermodynamics and dose-effect relationships in model wines revealed that binding between α-CD and malvidin-3- O -glucoside (Mvd-G) was a spontaneous exothermic reaction driven by enthalpy, with an optimal molar ratio of 1:5. Finally, structural characterization of host-guest complex and electronic effects of intermolecular interactions revealed that Mvd-G successfully formed host-guest complex with α-CD through hydrogen bonding and dispersion. The hydroxyl group and glucoside in the anthocyanin C ring, as well as the primary hydroxyl group of the α-CD were the key structures for forming hydrogen bonds. The results of this study provided an experimental and theoretical basis for the applications of α-CD as copigment to enhance the color quality and stability.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Yixin Liu,Zhiping Sun,Cunhui Wu,Xinguang Qin,Gang Liu,Xinlin Wei,Haizhi Zhang
Exploring efficient immobilized carrier for α-Amylase (α-Amy) to enhance its thermostability has significant influence in starch related industry. Here, hollow ZIF-8 (HZIF-8) with amino groups coated magnetic phase change microcapsules (PCM) was designed for covalent immobilization of α-Amy. Magnetic PCM consisting n-docosane core and SiO 2 /Fe 3 O 4 hybrid shell were firstly synthesized. Then, HZIF-8 shell with amino groups was coated and α-Amy was subsequently immobilized through covalent cross-linking strategy. The morphology, chemical structure and magnetic property of PCM@HZIF-8@α-Amy (PCMHA) were comprehensively characterized. Moreover, heat control property of PCMHA was studied with encapsulation efficiency and thermal energy-storage efficiency of 50.55 % and 50.59 %, respectively. Catalytic activity of immobilized α-Amy was fully investigated with K m and V max of 2.773 mg/mL and 1.853 μmol/mL·min, respectively. From reusability and storage stability study, immobilized α-Amy not only maintained rather high activity after 9 cycles' reuse, but also exhibited good activity under high salt ion condition after 7 days.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Li Yuan,Xueqian Wang,Xiang Li,Jianhong Wang,Langlang Wang,Yiran Xiong,Yuhang Xiao,Ping Ning
The performance of FeOOH for the removal of hydrogen sulfide (H 2 S) has been widely investigated. However, the harsh conditions of use and the difficulty of regeneration limit its practical application. In this study, FeOOH was loaded on activated carbon (AC) by a hydrothermal method to obtain a series of XF-AC catalysts for the removal of H 2 S from blast furnace gas (BFG). Among these catalysts, 3F-AC exhibited a high penetrating sulfur capacity of 482.3 mg/g and good regeneration performance. It is noteworthy that the use of AC as the support induced the transformation of α-FeOOH crystals into amorphous FeOOH and affected their morphology, and uniformly dispersed nanoparticles rather than agglomerated acicular crystals appeared on the surface of AC. The presence of amorphous FeOOH modulated the basic sites and surface hydroxyl groups of the catalysts while generating hydroxyl radicals during the reaction, thus improving the adsorption and oxidation of H 2 S. More importantly, the structural properties (surface area and pore volume) of 3F-AC enhanced the storage capacity of elemental sulfur and reduced the formation of by-products. The cyclic catalytic role of Fe 3+ /Fe 2+ played an important role in the oxidation process. This work provides a simple and effective strategy for the design of BFG desulfurization catalysts.
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