ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2023
·Vol. 415
·期 27
·6785-6798
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IF 4.3
Lu Zongyan,Guo Hao,Wang Mingyue,Yang Zeyun,Wei Xiaoqin,Liu Yinsheng,Hui Yingfei,Yu Zhiguo,Yang Wu
The development of a simple and sensitive electrochemical sensing platform for levofloxacin (LVF) analysis is of great significance to human health. In this work, a covalent organic framework (TP-COF) was in situ grown on the surface of Sn-MoC nanospheres with nanoflower-like morphology through a one-pot method to obtain the TP-COF@Sn-MoC composite. The prepared composite was used to modify a glassy carbon electrode (GCE) to realize the sensitive detection of levofloxacin. TP-COF was formed by polycondensation of 2,4,6-tris(4-aminophenyl)-1,3,5-triazine (TAPT) and pyromellitic dianhydride (PMDA), in which C = O and C = N groups served as double active centers for the recognition and electrocatalytic oxidation of the target molecule. Meanwhile, the introduction of Sn-MoC improved the conductivity of the electrode. The TP-COF@Sn-MoC composite produced a strong synergistic effect and showed a high electrocatalytic ability toward levofloxacin oxidation. The linear range of LVF was 0.6–1000 μM and the limit of detection (LOD) was 0.029 μM ( S / N = 3). In addition, the sensor has been successfully applied for the analysis of LVF in human urine and blood serum samples with acceptable recovery rates, demonstrating that the sensor was promising in practical applications. Graphical Abstract
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CHINESE JOURNAL OF POLYMER SCIENCE
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2023
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IF 4.3
Zhang Hao-Tian,Niu Ming-Xin,Zhang Qi,Hu Chen-Yang,Pang Xuan
Isothiourea is an important class of sulfur-containing molecules showing unique catalytic and biological activities. As such, polyisothiourea is envisioned to be an interesting type of polymer that potentially exhibits a number of interesting properties. However, there is no access to synthesizing well-defined polyisothiourea, and currently isothiourea-containing polymers are mainly prepared by immobilizing onto other polymer’s side chain. Herein, we report the first facile synthesis of polyisothioureas via alternating copolymerization of aziridines and isothiocayanates. Mediated by the catalytic system of phosphazene superbases/alcohol, a broad scope of aziridines and isothiocayanates could be transformed into polyisothioureas with adjustable substitutions (11 examples). The structures of obtained polyisothioureas were fully characterized with 1 H-NMR, 13 C-NMR, and 1 H- 13 C HMBC NMR. Moreover, the polyisothioureas show tunable thermal properties depending on substitutions on the isothiourea linkages. The novel structure of these polyisothioureas will enable a powerful platform for the discovery of next-generation functional plastics.
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JOURNAL OF SOLID STATE ELECTROCHEMISTRY
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2023
·
IF 2.5
Chen Linlin,Song Jiaqi,Wang Ling,Hao Xi,Zhang Haipeng,Li Xintong,Wu Jiashu
Nitrite ( \(\mathrm{{NO}_2}^{-}\) ) is often added to food as a food additive, but the overuse of nitrite will pose a threat to human health, so it is important to establish a rapid detection method for nitrite in related food. In this work, gold nanoparticles (AuNPs) were synthesized by in situ reduction of methanobactin (Mb); it is a small bioactive peptide. HS-β-cyclodextrin (HS-β-CD) with supramolecular recognition function was coordinated with Mb/AuNPs by Au-S bond to form HS-β-CD@Mb/AuNPs. HS-β-CD@Mb/AuNPs were modified onto the surface of gold electrode by electrodeposition to successfully prepare the biosensor (HS-β-CD@Mb/AuNPs/Au). The HS-β-CD@Mb/AuNPs/Au was characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), ultraviolet spectroscopy (UV), Fourier infrared spectroscopy (FTIR), fluorescence spectrometer (FL), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). The established biosensor was successfully applied to the detection of nitrite in real samples, and the mechanism of NO 2 − oxidation was analyzed. The detection limit of the established detection system was as low as 0.013 μM in the linear range of 0.1–10,000 μM, the recoveries were 96.53–102.54%, and the selectivity of the sensor is demonstrated by anti-interference experiment. Also compared to the previously described electrochemical sensors, the newly developed ones have better sensitivity (50.7137 μA μM −1 cm −2 ) and selectivity. These results suggest that the work provides a new way for the accurate monitoring of nitrite in environmental and food safety.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2023
·Vol. 30
·期 49
·108162-108175
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IF 5.8
Lu Yuanyuan,Pang Lina,Chatzisymeon Efthalia,Liu Xuna,Xu Kailin,Yang Ping,Gou Min
The metagenomics-based behavior and risk of antibiotic resistance genes (ARGs) were investigated during cattle manure thermophilic anaerobic digestion with tetracycline and copper, namely, bulk-copper oxide, nano-copper oxide, and copper sulfate, which are common feed additives. Although bulk-copper oxide reduced ARGs’ diversity, it enriched high-risk ARGs the most than the other two copper species, while copper sulfate could strongly mitigate the ARG risk by decreasing their abundances. Compared to corresponding individual effects, copper and tetracycline combinations may decrease ARGs’ co-occurrence potential by 22.0%, and particularly, tetracycline combined separately with copper sulfate and nano-copper oxide reduces the ARGs’ risk in abundance (by 7.2%) and human health (by 4.0%). These were mainly driven by bioavailable copper, volatile fatty acids, and pH, as well as the main potential hosts in phyla Firmicutes, Coprothermobacterota, and Euryarchaeota. Notably, the twin risks of pathogenicity and ARGs should be emphasized due to the ARGs’ positive correlation with human pathogens of Clostridioides difficile and Arcobacter peruensis . These findings are important for understanding the potential ARGs’ risk in treatments of livestock wastes containing feed additives of different sizes and speciation.
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JOURNAL OF MOLECULAR MEDICINE-JMM
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2023
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IF 4.7
Chen Yinghui,Wu Yizhuo,Feng Weiqi,Luo Xueyang,Xiao Bing,Ding Xiaowei,Gu Yongjia,Lu Yanan,Yu Yu
Abstract The ductus arteriosus (DA), bridging the aorta and pulmonary artery, immediately starts closing after birth. Remodeling of DA leads to anatomic obstruction to prevent repatency. Several histological changes, especially extracellular matrices (ECMs) deposition and smooth muscle cells (SMCs) migration bring to anatomic closure. The genetic etiology and mechanism of DA closure remain elusive. We have previously reported a novel copy number variant containing Vav2 in patent ductus arteriosus (PDA) patients, but its specific role in DA closure remains unknown. The present study revealed that the expression of Vav2 was reduced in human patent DA, and it was less enrichment in the adjacent aorta. Matrigel experiments demonstrated that Vav2 could promote SMC migration from PDA patient explants. Smooth muscle cells with Vav2 overexpression also presented an increased capacity in migration and downregulated contractile-related proteins. Meanwhile, SMCs with Vav2 overexpression exhibited higher expression of collagen III and lessened protein abundance of lysyl oxidase, and both changes are beneficial to DA remodeling. Overexpression of Vav2 resulted in increased activity of Rac1, Cdc42, and RhoA in SMCs. Further investigation noteworthily found that the above alterations caused by Vav2 overexpression were particularly reversed by Rac1 inhibitor. A heterozygous, rare Vav2 variant was identified in PDA patients. Compared with the wild type, this variant attenuated Vav2 protein expression and weakened the activation of downstream Rac1, further impairing its functions in SMCs. In conclusion, Vav2 functions as an activator for Rac1 in SMCs to promote SMCs migration, dedifferentiation, and ECMs production. Deleterious variant potentially induces Vav2 loss of function, further providing possible molecular mechanisms about Vav2 in PDA pathogenesis. These findings enriched the current genetic etiology of PDA, which may provide a novel target for prenatal diagnosis and treatment. Key messages
Although we have proposed the potential association between Vav2 and PDA incidence through whole exome sequencing, the molecular mechanisms underlying Vav2 in PDA have never been reported. This work, for the first time, demonstrated that Vav2 was exclusively expressed in closed DAs.
Moreover, we found that Vav2 participated in the process of anatomic closure by mediating SMCs migration, dedifferentiation, and ECMs deposition through Rac1 activation.
Our findings first identified a deleterious Vav2 c.701C>T variant that affected its function in SMCs by impairing Rac1 activation, which may lead to PDA defect. Vav2 may become an early diagnosis and an effective intervention target for PDA clinical therapy.
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Chinese Medicine
·
2023
·Vol. 18
·期 1
·1-14
·
IF 4.9
Guo Zhaopei,Wong Ka Hong,Li Enze,Zhou Xingzhi,Jiang Di,Gao Jiebing,Chen Meiwan
Background The integration of photodynamic therapy with a chemical drug-delivery system has displayed great potential in enhancing anticancer therapy. However, the solubility and non-specific biodistribution of both chemotherapeutic agents and photosensitizers continue to pose challenges that hinder their clinical applications.MethodA polypeptide-based nanoscale drug delivery system was fabricated to address the prementioned issues. An amphiphilic polymer was formed by conjugating the photosensitizer chlorin e6 (Ce6) onto a polypeptide poly-(L-lysine)-b-polyphenylalanine (PKF) for encapsulating the model drug dimeric camptothecin (DCPT), and the nanoparticles (PCD) with high drug loading efficiency were further modified with acid-sensitive polyethylene glycol (PEG) to yield the drug delivery sytem (PPCD).Results The DCPT and Ce6 encapsulation efficiency were analyzed as 99% and 73.5%, respectively. In phosphate-buffered saline (PBS) solution at a pH of 7.4, the PEG shell improved the stability of micelles and shielded their positive charge while in the acidic tumor microenvironment, the pH-sensitive PEG layer was removed to expose the cationic nanoparticles, thus facilitating the cellular uptake of PPCD micelles. Benefiting from the enhanced cellular internalization, the amount of intracellular reactive oxygen species (ROS) treated with PCD and PPCD micelles were obviously increased. Furthermore, the enhanced anti-cancer efficacy prompted by PPCD micelles was validated through cellular and animal study.Conclusion This study presents a promising method to promote the solubility and biodistribution of both chemotherapeutic agent and photosensitizer, thereby facilitating the further application of chemo-photodynamic cancer therapy.Graphical Abstract
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Journal of Sustainable Metallurgy
·
2023
·Vol. 9
·期 4
·1456-1465
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IF 2.4
Li Hui-Fang,Li Li-Juan,Ji Lian-Min,Shi Dong
To develop a more stable and more efficient extraction system, a novel amide extraction system, containing extractant N , N -bis(2-ethylhexyl) acetamide (N523) and diluent diisobutyl ketone (DIBK), was proposed in this work. A series of operation conditions, such as the concentration of initial lithium in aqueous phase, the volume ratio of N523 and DIBK, phase ratio of O / A , the molar ratio of Fe/Li, were carried out. The lithium extraction rate reached up to 92% under the optimal extraction conditions. Considering the mass transfer of two phases and consumption of extractant, the three-stage counter-current extraction experiment was conducted. After 6 cycles experiment of the three stage counter-current extraction, the lithium extraction rate reached 99.89% and the separation factor of Li/Mg, Li/Na, Li/K, and Li/Ca were 103,300, 5288, 5233, and 760.4, respectively. The stoichiometry of the complex was LiFeCl 4 ·N523 by the slope method. The results indicated that N523-DIBK extraction system was effective to recovery of lithium from salt lake brine with high mass ratio of Mg/Li. Furthermore, the extraction thermodynamics study revealed lithium extraction was an exothermic process, which meant lower temperature promoted lithium extraction. Graphical Abstract
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Nano-Micro Letters
·
2023
·Vol. 15
·期 1
·1-15
·
IF 26.6
Yu Zhen,Su Yuqing,Gu Ruonan,Wu Wei,Li Yangxi,Cheng Shaoan
Micro–nano water film enhanced interfacial solar evaporator enables a high evaporation rate of 2.18 kg m −2 h −1 under 1 sun.
An outdoor device with an enhanced condensation design demonstrates a high water production rate of 15.9–19.4 kg kW −1 h −1 m −2 .
A multi-objective predictive model is established to assess outdoor water production performance.
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POLYMER BULLETIN
·
2023
·
IF 3.2
Ma Puhao,Lu Wentong,Chen Jiacheng,Tian Hao,Wang Jincheng,Xiao Jianhua
The industry’s mechanical equipment often faces a significant demand for damping rubber. Simultaneously improving damping and toughness of rubber materials is a challenging task. It was achieved by incorporating crystal particles into the chlorinated butyl rubber (CIIR) network, inspired by the “sliding crystal mechanism”. To create the elastomers with high damping and toughness, polyurethane was used along with polyethylene glycol as a soft segment. The combination factors, including coordination and hydrogen bonds, played a crucial role in facilitating the formation of crystal particles within the material. These crystal particles acted as rigid fillers, enhancing the material’s toughness. Additionally, the sacrificial reversible bonds present in the crystal particles allowed for energy dissipation during stress. These bonds can be repeatedly broken and regenerated, enabling the material to dissipate energy efficiently. Comparing these elastomers to pure CIIR, they exhibited a tensile strength and elongation at break of 5.76 MPa and 1973%, a fracture energy of 28.73 MJ/m 3 , a damping factor of 1.52, and a damping temperature range of 80 °C. These results indicate significant improvements in both damping and toughness properties. This advancement could have valuable implications for the industry’s mechanical equipment, which often relies on effective damping rubber.
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Cancer Cell International
·
2021
·Vol. 21
·期 1
·1-13
·
IF 5.722
Zheng Kebin,Xie Haipeng,Wu Wensong,Wen Xichao,Zeng Zhaomu,Shi Yanfang
Background Increasing studies have revealed that circular RNAs (CircRNAs) make great contributions to regulating tumor progression. Therefore, we intended to explore the expression characteristics, function, and related mechanisms of a novel type of circRNA, PIP5K1A, in glioma.Methods Firstly, reverse transcription-polymerase chain reaction (RT-PCR) was carried out to examine CircPIP5K1A expression in glioma tissues and adjacent normal tissues, and the correlation between CircPIP5K1A level and the clinical-pathological indicators of glioma was analyzed. Then, the CircPIP5K1A expression in various glioma cell lines was detected, and CircPIP5K1A overexpression and knockdown cell models were constructed. Subsequently, cell proliferation and viability were detected by the CCK8 method and BrdU staining. Cell apoptosis was detected by flow cytometry, and cell invasion was examined by Transwell assay. The expression of TCF12, PI3K/AKT pathway apoptotic related proteins (Caspase3, Bax, and Bcl2) and epithelial-mesenchymal transition (EMT) markers (E-cadherin, Vimentin, and N-cadherin) was determined by western blot or RT-PCR.Results The results manifested that CircPIP5K1A was upregulated in glioma tissues (compared with that in normal adjacent tissues), and overexpressed CircPIP5K1A was related to glioma volume and histopathological grade. Functionally, overexpressing CircPIP5K1A notably elevated glioma cell proliferation, invasion, and EMT and inhibited apoptosis both in vivo and in vitro. Besides, CircPIP5K1A upregulated TCF12 and PI3K/AKT activation. Bioinformatics analysis testified that miR-515-5p was a common target of CircPIP5K1A and TCF12, while the dual-luciferase reporter assay and RNA immunoprecipitation (RIP) experiment further confirmed that CircPIP5K1A targeted miR-515-5p, which bound the 3′-untranslated region (UTR) of TCF12.Conclusion sOverall, the study illustrated that CircPIP5K1A is a potential prognostic marker in glioma and regulates glioma evolvement by modulating the miR-515-5p-mediated TCF12/PI3K/AKT axis.
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Advanced Fiber Materials
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2023
·
IF 16.1
Mu Jiaxiang,Meng Zhaoxu,Liu Xinran,Guan Ping,Lian He
Photothermal therapy (PTT) has been proposed as an advanced patient-centered strategy for tumor treatment. Nevertheless, the uncertain safety of conventional photothermal conversion agents and the presence of intracellular self-protective autophagy mechanisms pose obstacles to the clinical application and efficacy of PTT. As we are deeply aware of the seriousness of these problems, we herein proposed an efficacy-enhancing strategy based on an implantable membrane platform (PPG@PB-HCQ) constructed from poly (lactic acid) (PLA), poly (ɛ-caprolactone) (PCL) and gelatin (Gel) electrospun nanofibers (PPG) and loaded with the biodegradable high-efficiency photothermal conversion agent Prussian blue (PB) and the autophagy inhibitor hydroxychloroquine sulfate (HCQ). Cellular experiments confirmed that the PPG@PB-HCQ nanofiber membrane exhibited a significantly stronger tumor cell-killing effect compared with the PTT alone. This enhancement features by of blocking the fusion of autophagosomes with lysosomes. The intracellular overexpression of the proteins microtubule-associated protein 1 light chain 3 (LC3)-II and p62 and the low expression of the proteins LC3-I and Rab7 (members of the RAS oncogene family) further demonstrated autophagic flux blockade. Importantly, the potent antitumor effect of the PPG@PB-HCQ therapeutic platform in B16 tumor-bearing model mice verified the efficacy-enhancing strategy of synergistic PTT and protective autophagy blockade. The present study provides a promising strategy for solving the difficulties of tumor treatment, as well as a new perspective for designing novel treatment platforms. Graphical Abstract
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2023
·Vol. 30
·期 39
·91262-91275
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IF 5.8
Cheng Tangying,Li Jinjin,Ma Xiuwei,Yang Linjun,Zhou Lei,Wu Hao
Various materials have been developed to capture volatile organic compounds (VOCs) to mitigate air pollution. However, sorbent materials with excellent resistance to water are rare. Here, several Fe/N-doped activated carbons (ACs) have been prepared to capture VOCs in humid environments. The ACs were analyzed by various characterization techniques, such as BET, SEM, XPS, XRD, FTIR, and Raman. The results showed that Fe/N doping resulted in the specific surface area of the ACs increasing by 500 to 1000 m 2 g −1 , the average pore size increasing to approximately 2 nm, improved mesoporous structure, higher graphitization, lower hydrophilicity, and polarity. The VOCs adsorption performance of the ACs was evaluated by static and dynamic adsorption experiments. The uptake of toluene and ethyl acetate by ACs was enhanced to 224 mg g −1 and 135 mg g −1 , respectively. And ACs were able to maintain 70 to 80% VOCs adsorption capacity for VOCs at 80% relative humidity. Furthermore, the microscopic mechanisms were investigated by the grand canonical Monte Carlo method (GCMC). The highly graphitized structure and the N functional groups favored the VOC adsorption process and discouraged the adsorption of water vapor. This work affirmed the dominance of Fe/N-doped carbon, which will contribute to the evolution of water-resistant VOCs adsorbent materials. Graphical Abstract
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危险品化学品经营许可证（带存储）