JOURNAL OF FLUORESCENCE
·
2024
·
IF 2.6
Dou Liwei,Xu Lijun,Gao Hong,Song Jie,Shang Shibin,Song Zhanqian
In this paper, molecules with AIE red light properties were designed by coupling dehydroabietic acid diarylamine and 2,3-diphenylfumaronitrile, which were designated 2DTPA-CN and 2TPA-CN. The emission wavelengths were 683 nm and 701 nm, respectively. The 2DTPA-CN and 2TPA-CN showed typical AIE characteristics with large Stokes shifts of 7.4 × 10 4 cm -1 and 6.7 × 10 4 cm -1 , respectively. The obvious solvatochromism and electron cloud distributions of HOMO/LUMO in the ground and excited states both reveal the intramolecular charge transfer (ICT) effect. The 2DTPA-CN, boasting exceptional biocompatibility, was successfully prepared into nanoparticles (NPs), which were applied to tumor cell imaging, showing good bioimaging effects both in vitro imaging in live cells and in vivo imaging in live mice. The results demonstrated that it possesses significant potential as an effective bioimaging reagent for the detection of tumor cells. Furthermore, the incorporation of 2,3-diphenylfumaronitrile moieties to dehydroabietic acid diarylamine emerged as a proficient approach to broaden the emission wavelengths of rosin-based fluorescent materials.
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TALANTA
·
2024
·
IF 5.6
Xiaoming Li,Jiaqi Hu,De Zhang,Xiubin Zhang,Zhetao Wang,Yufeng Wang,Qiang Chen,Pei Liang
Surface-enhanced Raman spectroscopy (SERS) can quickly identify molecular fingerprints and has been widely used in the field of rapid detection. However, the non-uniformity inherent in SERS substrate signals, coupled with the finite nature of the detection object, significantly hampers the advancement of SERS. Nowadays, the existing mature immunochromatographic assay (ICA) method is usually combined with SERS technology to address the defects of SERS detection. Nevertheless, the porous structure of the strip will also affect the signal uniformity during detection. Obviously, a method using SERS-ICA is needed to effectively solve signal fluctuations, improve detection accuracy, and has certain versatility. This paper introduces an internal standard method combining deep learning to predict and process Raman data. Based on the signal fluctuation of single-antigen SERS-ICA test strip, the double-antigen SERS-ICA test strip was constructed. The full spectrum Raman data of double-antigen SERS-ICA test strip was normalized by the sum of two characteristic peaks of internal standard molecules, and then processed by deep learning algorithm. The Relative Standard Deviation (RSD) of Raman data of bisphenol A was compared before and after internal standard normalization of double-antigen SERS-ICA test strip. The RSD processed by this method was increased by 3.8 times. After normalization, the prediction accuracy of Root Mean Square Error (RMSE) is improved by 2.66 times, and the prediction accuracy of R-square (R 2 ) is increased from 0.961 to 0.994. The results showed that RMSE and R 2 were used to comprehensively predict the collected data of double-antigen SERS-ICA test strip, which could effectively improve the prediction accuracy. The internal standard algorithm can effectively solve the challenges of uneven hot spots and poor signal reproducibility on the test strip to a certain extent, so as to improve the semi-quantitative accuracy.
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ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Ce Chen,Lidong Yu,Xueting Li,Zewen Yu,Danjie Song,Siqi Wang,Fangshun Li,Shanshan Jiang,Yajie Chen,Jucai Xu,Jianglin Fan,Bingsheng Li,Li Li
Ultraviolet (UV) exposure causes damage to human skin and mucous membranes, resulting in oxidative stress, and can also lead to inflammation of human skin, skin aging, and even diseases such as squamous cell carcinoma and melanoma of the skin. The main means of protection against UV radiation is physical shielding and the use of sunscreen products. Carbon dots as a novel nanomaterial provide a new option for UV protection. In this article, we introduced sulfhydryl groups to synthesize l-cysteine-derived carbon dots (GLCDs) with UV resistance. GLCDs exhibit high-efficiency and excellent UV absorption, achieving 200–400 nm UV absorption (99% UVC, 97% UVB, and 86% UVA) at a low concentration of 0.5 mg/mL. Meanwhile, GLCDs can reduce apoptosis and UVB-induced oxidative damage, increase collagen type I gene expression, and inhibit skin aging in zebrafish. It also inhibits senescence caused by the senescence inducer 2,2′-azobis(2-methylpropionamidine) dihydrochloride and reduces oxidative damage. The above studies show that GLCDs possess efficient broad-spectrum UV absorption, antiphotoaging, and antiaging capabilities, which will have a broad application prospect in UV protection.
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FITOTERAPIA
·
2025
·
IF 2.6
Li Zhang,Hui Wu,Xiaoyuan Li,Xiu Zhang,Hua Li,Huaqiao Tang,Daxuan Tang,Shijing Du,Ya’’ou Liu,Yangfan Tang,Xiaoming Bao,Guoqiang Cheng
Qianghuo (the rhizomes and radixes of Notopterygium incisum Ting ex H.T. chang) is a traditional Chinese medicine, and widely used as an anti-inflammatory, anodyne, antifebrile agent. Qianghuo volatile oil also exhibits the same properties, and the effects are probably related to the inhibition of cyclooxygenase (COX), but its bioactive components are not clear yet. To figure out the bioactive components of Qianghuo volatile oil target to cyclooxygenase-2 (COX-2), a ligand fishing method using immobilized COX-2 magnetic beads (COX-2-MB S ) and gas chromatography–mass spectrometry (GC–MS) were developed to identify the compounds that act on COX-2. Nine ligands, including 3-carene, β-caryophyllene, eucalyptol, α-terpinene, Limonene, δ-terpinene, α-pinene, β-pinene and γ-terpinene, were specifically captured by COX-2-MB S . Among them, 3-carene, β-caryophyllene, and eucalyptol in Qianghuo volatile oil were isolated and confirmed by in vitro inhibition and in vivo anti-inflammatory activity assays as selective inhibitor against COX-2. The IC 50 values were 13.5, 10.1, and 7.6 μM, respectively. Compared with the model, 3-carene, β-caryophyllene, and eucalyptol considerably reduced ear swelling and paw edema. Moreover, 3-carene and eucalyptol significantly decreased the changes of COX-2 expression in RAW264.7 cells activated by LPS. Our study validated that the developed ligand-fishing method using COX-2-MBs coupled with GC–MS can be applied to recognize COX-2 inhibitors in Qianghuo volatile oil. In vitro/vivo assay showed 3-carene and eucalyptol in Qianghuo volatile oil had strong inhibition against COX-2. This study may provide novel methods for discovering active components for volatile oil–based drug development or aromatherapy.
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MOLECULES
·
2025
·Vol. 30
·期 1
·187
·
IF 4.6
Shuqi Liao,Weisen Deng,Feng Yang,Jutao Zhou,Ling Wu,Donghong Yu,Zhong Cao
Compared to natural enzymes, the development of efficient artificial simulated enzymes, such as those based on bimetallic materials with high catalytic activity and good stability, is an important way until now. Herein, we employed ZnCo2O4microspheres as carriers to synthesize Pt-doped composites with different amounts using a one-pot method. The morphology and structure of the synthesized materials were characterized using XRD, SEM, BET, FT-IR, XPS, and Zeta potential techniques. It was found that Pt0adhered well to the surface of ZnCo2O4microspheres, with a 12.5% Pt doped ratio exhibiting abundant oxygen vacancies, excellent substrate affinity, and high peroxidase-like activity. Using fluorescent probes and electrochemical methods, the peroxidase-like catalytic mechanism has been explored that Pt@ZnCo2O4microspheres can accelerate the electron transfer between H2O2and 3,3′,5,5′-tetramethylbenzidine (TMB). Based on the optimal loading ratio of 12.5% of Pt@ZnCo2O4, a colorimetric sensor for visual detection of L-cysteine (L-Cys) was constructed, exhibiting a wide linear range of 0.1~50 µM and a low detection limit of 0.0163 µM. The sensor possesses good selectivity, reusability, and usage stability, which can be well applied to the determination of L-Cys in health product capsules with recovery rates of 96.9%~103.7% and RSD of 1.07%~6.50%. This work broadens the application prospects of spinel materials such as ZnCo2O4in the field of biological analysis and also provides inspiration for the development of new artificial simulated enzymes.
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COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
·
2025
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IF 4.3
Hongming Tang,Juan Liu,Dong Sheng,Xu Ren,Qinghua Yu,Zhixin Guo,Yunpeng Wu,Yuxiao Liu,Yuehan Xiao,Wei Wang,Shigen Ye,Weijia Zhou
Isobavachalcone (IBC), a bioactive flavonoid derived from Psoralea corylifolia , exhibits potent anti-ciliate activity, but its underlying mechanism remains unclear. Utilizing Tetrahymena thermophila as a model organism, we demonstrated that IBC induces dose-dependent mortality (12 h-IC 50 : 1.39 mg/L) and inhibits growth. Mechanistically, IBC triggers oxidative stress by elevating reactive oxygen species (ROS) and disrupting antioxidant enzymes, including superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione (GSH). This disruption leads to membrane damage, as evidenced by lactate dehydrogenase (LDH) leakage and ATPase inhibition, as well as mitochondrial dysfunction. Microscopic examination and staining confirmed that cell death occurs via necrotic cell death rather than apoptosis. Transcriptome analysis revealed key pathways, including peroxisome-mediated oxidation, glutathione metabolism, and ATP-binding cassette (ABC) transporters, further supporting the role of IBC in oxidative and structural disruption. These findings elucidate the anti-ciliate mechanism of IBC, providing valuable insights for developing targeted anti-parasitic agents.
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ADVANCED MATERIALS
·
2025
·
IF 26.8
Yaqing Kang,Xiaoqing Han,Shijie Zhou,Xingbo Wang,Yanjing Wang,Panpan Song,Xiaochen Su,Mengmeng Qin,Dongyang Qian,Huan Meng,Jiao Yan,Fang Pu,Haiyuan Zhang
Rheumatoid arthritis microenvironment (RAM) contains complex pathogenic mediators that interact dynamically to drive the progression of rheumatoid arthritis (RA). However, most current RA treatments are single-target interventions, exerting limited impact on RAM. Herein, apoptotic extracellular vesicles (ApoEV) are constructed for programmable regulation of the neutrophil-macrophage-ROS pathogenic axis, aiming to reconstruct RAM and improve RA therapy. Mesenchymal stem cells (MSCs) are pretreated with dexamethasone (Dex) and induced apoptosis to produce Dex-loaded and FasL-overexpressing ApoEV (D@ApoEV FasL ), which is further modified with low-molecular-weight heparin (LMWH) through a ROS-responsive cleavage linker to form D@ApoEV FasL ∩L. After intravenous injection into RA mice, D@ApoEV FasL ∩L targeted the inflamed joints based on their MSC-derived feature and blocked neutrophil recruitment through binding to P-selectin on vascular endothelial cells. In response to high ROS, D@ApoEV FasL ∩L shed LMWH and exposed FasL, inducing neutrophil apoptosis through the Fas/FasL signaling pathway. Subsequently, the apoptotic neutrophils triggered macrophage reprogramming from M1 to M2 phenotype, and the released Dex significantly reduced the oxidative damage. Various in vitro and in vivo assessments have confirmed that D@ApoEV FasL ∩L can effectively regulate neutrophils, macrophages, and ROS, trigger an immune cascade, and restore intra-articular immune homeostasis, exhibiting an effective RAM reconstruction ability and a promising therapeutic effect for RA.
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Talanta Open
·
2025
·
IF 3.7
Qiao Chen,Xiaoran Zhang,Xinyue Zhou,Peng Huang,Daming Feng,Xu Xu,Chunhua Ge
We report a metal-free perylene-based polyimide framework (PDI-PB) as an efficient electrocatalyst for oxygen reduction reaction (ORR)-enhanced cathodic electrochemiluminescence (ECL). PDI-PB enhances reactive oxygen species (ROS) generation via oxygen reduction that significantly amplify the luminol–dissolved oxygen ECL signal. The resulting biosensor enables ultrasensitive detection of dopamine (DA) over a wide linear range (5 × 10 -5 to 100 μM) with a record-low detection limit of 9.76 pM (S/N = 3). The sensor exhibits excellent selectivity, stability, and reproducibility, and was successfully applied to detect DA in human sweat, serum, and urine, yielding recoveries of 97.0–101.1%. Density functional theory (DFT) calculations reveal that PDI-PB’s superior electronic structure facilitates efficient charge transfer and oxygen activation. This work presents a new strategy for metal-free organic electrocatalyst design, combining mechanistic insight with high-performance ECL biosensing for non-invasive health monitoring and clinical diagnostics.
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TALANTA
·
2025
·
IF 6.1
Ruoxuan Jiang,Lei Pan,Fan Yang,Shudong Zhang,Dan Lin,Changlong Jiang
The detection of biomarkers is of great significance in the assessment of physical condition and disease progression. Developing rapid reliable biomarker detection technologies is necessary to meet the increasing health monitoring demands. Here, we developed an ultrasensitive sensing system for determining uric acid (UA), constituted by the metal-organic frameworks structure MIL-100(Fe)@MOF-Tm. The sensing mechanism is based on the enzymatic oxidation reaction catalyzed by uricase. Owing to the ideal loading capacity, the as-designed MOF-on-MOF system could load plentiful uricase to maximize the enzymatic reaction effect. The possible catalytic enhancement triggered by surface metal sites enables ultrasensitive and efficient response to the target analyte. With increasing UA concentration, uricase loaded on the MOF structure generates allantoin and H 2 O 2 . The Fe 3+ is reduced and undergoes the Fenton reaction, promoting the conversion from o -phenylenediamine (OPD) to 2,3-diaminophenazine (DAP). Consequently, the sensor changes from blue to yellow fluorescence under UV and becomes deep yellow from colorless under natural light, respectively, achieving dual-model visual detection. Thanks to the reusable feature and stability, this sensor could be distinguished from other one-time probes with extraordinary reusage, selectivity, and low detection limit (3 nM) to UA. For the inherently suitability for constructing devices, the paper-based sensor and hydrogel sensing patch were developed and applied in multiple real samples detecting. This study formulates a reliable strategy for quantitatively monitoring UA, and furnishes new avenues for personal medical sensors.
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BIOMATERIALS
·
2025
·
IF 12.9
Zhanxue Xu,Xinrui Yang,Xingyu Lu,Dandan Su,Yidan Wang,Huixing Wu,Zhenhua Zhang,Changrui Long,Liqian Su,Yanyu Wang,Hongbo Chen,Shijian Xiang,Benjie Zhou
Combination therapies for melanoma face challenges due to asynchronous drug delivery and associated toxicity, underscoring the need for advanced delivery systems. While immune checkpoint inhibitors (ICIs) enhance T cell activity, optimal cytotoxic responses require efficient antigen presentation by mature dendritic cells (DCs), which are often functionally impaired in the tumor microenvironment. Thus, effective treatment requires coordinated T cell activation, DC-mediated priming, and direct tumor suppression. Herein, wild Glycyrrhiza uralensis Fisch roots-derived nanovesicles (GC NV) are demonstrated to be effective inhibitors of melanoma proliferation. The vesicles exert this activity through the intracellular delivery of encapsulated miRNA (miR2916) and bioactive molecules (isoliquiritigenin), with this capacity for intracellular delivery extending to the STING agonist DMXAA. We also demonstrate how chemical modification can be used to install PD-L1 antibodies on the membrane surface of these GC NV, imbuing these vesicles with selectivity for tumor cells. Combining DMXAA encapsulation with surface-displayed PD-L1 antibodies creates vesicles (GP@DMX NV) that both promote DCs maturation and elicit CD8 + T cell response. Our multifunctional GP@DMX NV reverse the immunosuppressive microenvironment of melanoma and significantly enhance the immunotherapeutic potential of immune checkpoints.
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BIOMACROMOLECULES
·
2015
·
IF 5.75
Menglin Wang,Jin Sun,Yinglei Zhai,He Lian,Cong Luo,Lin Li,Yuqian Du,Dong Zhang,Wenya Ding,Shuhong Qiu,Yuhai Liu,Longfa Kou,Xiangfei Han,Rongwu Xiang,Yongjun Wang,Zhonggui He
To improve the bioavailability of orally administered drugs, we synthesized a pH-sensitive polymer (poly(ethylene glycol)–poly(2-methyl-2-carboxyl-propylene carbonate)–vitamin E, mPEG–PCC–VE) attempting to integrate the advantages of enteric coating and P-glycoprotein (P-gp) inhibition. The aliphatic polycarbonate chain was functionalized with carboxyl groups and vitamin E via postpolymerization modification. Optimized by comparison and central composite design, mPEG113–PCC32–VE4 exhibited low critical micelle concentration of 1.7 × 10–6 mg/mL and high drug loading ability for tacrolimus (21.2% ± 2.7%, w/w). The pH-responsive profile was demonstrated by pH-dependent swelling and in vitro drug release. Less than 4.0% tacrolimus was released under simulated gastric fluid after 2.5 h, whereas an immediate release was observed under simulated intestinal fluid. The mPEG113–PCC32–VE4 micelles significantly increased the absorption of P-gp substrate tacrolimus in the whole intestine. The oral bioavailability of tacrolimus micelles was 6-fold higher than that of tacrolimus solution in rats. This enteric polymer therefore has the potential to become a useful nanoscale carrier for oral delivery of drugs.
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AAPS PHARMSCITECH
·
2019
·Vol. 20
·期 2
·1-15
·
IF 2.608
Zhang Shudong,Wan Qiang,Xing Yidan,Ding Jiafeng,Yang Shizhuang,Sun Weiwei,Lu Mengmeng,Pan Baoliang
Drugs with pH-dependent solubility that have poor water solubility can be identified in the drug discovery pipeline. Some of them have poor oral absorption, which can result in insufficient efficacy. Micro-environmental pH-modifying solid dispersion (micro pHm SD) is a promising approach to overcome the poor oral absorption of these drugs. In the present study, toltrazuril (TOL), a weakly acidic drug with poor aqueous and pH-dependent solubility, was used as a model drug. Using micro pHm SD, a novel oral oil-based suspension of TOL SD (TSDS) was developed, and the stability of this formulation was evaluated based on particle size, settling volume ratio, redispersibility, thermal stability, and drug content. The optimized soybean oil-based TSDS (S-TSDS) had high physicochemical stability and good histocompatibility with common inflammatory reactions. The results of the in vitro dissolution analysis showed that S-TSDS rapidly and markedly released the drug and provided higher efficacy and longer persistence against coccidiosis (above 90.9%) in rabbits. This technique could increase the oral absorption and bioavailability of new drug candidates.
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危险品化学品经营许可证（带存储）