MICROCHEMICAL JOURNAL
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2026
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IF 5.2
Jinxuan Li,Shuimiao Zhang,Xiaolin Zhu,Jiao Qu
First electrochemical DNA sensor for detecting vancomycin resistance genes vanA / vanB . • Synergistic AuNPs/IL-CNT nanocomposite boosts signal amplification and probe loading. • Wide linear range (0.1 pM-10 nM) with an ultra-low detection limit of 0.1 pM. • Validation in real water samples shows 96.4–106.9% recovery and correlates with qPCR. • High specificity, good stability (RSD < 2.17%), detects variable-length fragments. The widespread occurrence of antibiotic resistance genes (ARGs) has become a severe global threat to environmental and public health. The detection of vancomycin resistance genes (VRGs) is particularly critical, given vancomycin's role as a last-line agent against multidrug-resistant Gram-positive bacteria. In this study, we developed a novel electrochemical DNA sensor for the ultrasensitive detection of VRGs, representing a novel electrochemical approach for VRG detection with significant performance advantages over existing methods for ARGs. The sensor incorporated a rationally designed layered interface. This interface was formed by immobilizing ionic liquid-functionalized carbon nanotubes (IL-CNT) onto a glassy carbon electrode to form a highly conductive substrate, which facilitated electron transfer and served as an ideal platform for the subsequent electrodeposition of gold nanoparticles (AuNPs). The resulting AuNPs/IL-CNT nanocomposite significantly increased the electroactive surface area and provided abundant sites for anchoring thiolated van A-specific single-stranded DNA probes. This synergistic multi-component architecture significantly enhanced both probe loading capacity and electrochemical signal transduction efficiency. The sensor demonstrated outstanding performance, including an exceptionally low detection limit of 0.1 pM, a short assay time of 40 min, high reproducibility (RSD < 1.18%), excellent selectivity, and good stability (RSD < 2.17%). Furthermore, results from detecting van A in various water samples were highly consistent with quantitative real-time polymerase chain reaction. Hence, this work presents a rapid, reliable, and highly sensitive platform for monitoring ARGs in real-world aquatic environments. Download: Download high-res image (128KB) Download: Download full-size image
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MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Yan Zeng,Peng Qi,Yan Sun,Yijun Lin,Peng Wang
Programmable DNA-assembled SERS nanotag enables simultaneous target capture and signal amplification. • Dual-mode LFA merges rapid visual screening with ultrasensitive SERS quantification on one platform. • Robust performance in complex clinical samples supports point-of-care infectious disease diagnostics. • Simultaneous dual-bacteria detection via LFIA achieved 20 min visual readout and 10 cfu/mL sensitivity under SERS system. The persistent threat of infectious diseases underscores the critical need for point-of-care diagnostic tools that are both rapid and ultrasensitive. This study addresses this challenge by developing a novel dual-mode lateral flow assay (LFA) platform powered by a programmable, DNA framework-assembled surface-enhanced Raman scattering (SERS) nanotag. The innovation lies in the rational integration of a high-load SERS signal core and a tailored plasmonic enhancer through DNA nanotechnology. First, a three-dimensional rolling circle amplification (RCA)-DNA nanoassembly scaffold was fabricated RCA reaction. Pre-synthesized Raman signal probes conjugated with gold nanoparticles were then immobilized onto this scaffold. Because the RCA template had been designed to include bacteria-specific primers, the assembled DNA-based SERS nanotag exhibits both the ability to specifically capture target microorganisms and the capacity for strong SERS signal output. When deployed on a standard LFA strip, this nanotag enables two distinct yet complementary detection modes: a rapid colorimetric readout for immediate, instrument-free screening and a quantitative SERS measurement for ultrasensitive confirmation. The platform enabled rapid visual detection via test strips within 20 min and achieved single-digit colony SERS quantification, demonstrating an ultrasensitive detection limit while retaining high specificity and robustness in complex sample matrices. This work establishes a versatile and powerful paradigm for next-generation point-of-care diagnostics, merging the convenience of rapid testing with the precision of laboratory-grade quantification, and highlights the transformative potential of synthetic biology in advanced biosensing. Download: Download high-res image (104KB) Download: Download full-size image
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MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Shulan Chen,Jiang Huang,Wenyue Chen,Hongwen Wu,Hongliang Tan
Hybridization gating reduces fluorophore ratio-control constraints. • Post-activation dye recruitment limits background from pre-integrated signal dyes. • Immunomagnetic enrichment provides target selectivity for ATP-based sensing. • Chitosan-modified hydrogel limits acid-induced background in portable readout. Rapid bacterial detection in dairy food safety monitoring requires portable platforms that combine analytical sensitivity with target selectivity. Here, we report a hybridization-gated zeolitic imidazolate framework-8 (ZIF-8) ratiometric nanoprobe, designated RSZ, for portable detection of Staphylococcus aureus ( S. aureus ) through adenosine triphosphate (ATP)-triggered DNA duplex signaling. The RSZ probe adopts a dual-zone architecture with Ru(bpy) 3 2+ and ssDNA1 embedded within the ZIF-8 particle interior and ssDNA2 adsorbed on the exterior surface. This architecture separates the stable reference channel from the formation of the responsive fluorescence channel, thereby reducing dependence on precise dual-fluorophore loading and limiting background associated with a pre-integrated responsive fluorophore. Upon ATP-induced framework disruption, ssDNA1 becomes accessible for hybridization with ssDNA2, and the resulting duplex recruits SYBR Green I from solution to generate the responsive fluorescence channel relative to the stable Ru(bpy) 3 2+ reference. To enable field-deployable detection, the nanoprobe is embedded in a chitosan-modified agarose hydrogel in which chitosan maintains a near-neutral microenvironment and limits acid-induced nonspecific activation. Fe 3 O 4 nanoparticles conjugated with porcine immunoglobulin G (Fe 3 O 4 -pIgG) are integrated upstream to selectively capture S. aureus via protein A-pIgG recognition, followed by smartphone red-green-blue analysis without specialized instrumentation. The integrated platform achieves a detection limit of 6 CFU/mL and recoveries of 98.8–101.7% in spiked milk. Therefore, this study demonstrates a hybridization-gated strategy for translating stimulus-induced ZIF-8 disruption into controlled ratiometric signal generation for portable bacterial sensing in food analysis. Download: Download high-res image (106KB) Download: Download full-size image
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2025
·
IF 9.7
Lulu Liu,Ruoyao Li,Lijuan Xie,Xinxin Zhang,Deyan Gong,Zhengbao Zha,Cheng-Yan Xu,Zhaohua Miao
Inflammatory bowel disease (IBD), a chronic inflammatory gastrointestinal disease, is closely related to diverse inflammatory factors like reactive oxygen and nitrogen species (RONS) and cell-free DNA (cfDNA). Herein, tannic acid (TA) modified hafnium hydride nanosheets (HfH 1.983 @TA NSs) with a thickness of 1–4 nm, prepared by liquid-phase exfoliation, were explored as a high-performance anti-inflammatory agent for IBD treatment. By virtue of the high specific surface area of two-dimensional structure, HfH 1.983 @TA NSs can effectively not only scavenge RONS, but also eliminate cfDNA, thus inhibiting the pro-inflammatory responses. In a dextran sodium sulfate (DSS)-induced IBD mouse model, HfH 1.983 @TA NSs by oral administration rapidly alleviated various IBD symptoms, including disease activity index (DAI), colon length, and the level of pro-inflammatory cytokines in 5 days, superior to the clinical drug 5-aminosalicylic acid (5-ASA). This work introduces ultrathin hafnium hydride nanosheets with RONS and cfDNA-dual scavenging capabilities as efficient two-dimensional anti-inflammatory agents for IBD treatment.
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JOURNAL OF CONTROLLED RELEASE
·
2025
·
IF 11.5
Cheng Chen,Qingyun Wei,Zhen Zhu,Wenhui Wu,Tiantian Gong,Yangbo Lv,Aimei Zhang,Chunping Hu,Qichen Zhan,Tao Cao,Peng Cao
Controlled drug delivery systems not only augment the therapeutic efficacy of drugs but also mitigate their adverse effects. Methotrexate (MTX) is a prominent first-line therapeutic agent in the management of rheumatoid arthritis (RA), yet it is characterized by its limited aqueous solubility, pronounced hemolytic activity, and propensity for off-target binding, which collectively contribute to its significant toxicity profile. To address these issues, a temperature-responsive controlled release system for MTX based on the analogous base pairing rule was designed for the RA treatment. Magnetic ferroferric oxide nanoparticles (Fe 3 O 4 NPs) coated with polydopamine (PDA) were modified with thymidine-1-acetic acid (TAA) to prepare nanocarrier (FPT NPs). Furthermore, the thymine molecules within the FPT NPs possessed the strong ability to specifically bind with the pteridine structure present in MTX, thereby enabling the fabrication of a nanomedicine (designated as FPT-MTX NPs) through the base pairing rule. Under the influence of an applied magnetic field, the FPT-MTX NPs demonstrated a remarkable capacity for precisely targeting the joint tissue in a mouse model of RA. Analogous to DNA, where double-strand breaks occurred due to heating-induced disruption of base pairing, MTX was released from FPT-MTX NPs following near-infrared irradiation-induced light-to-heat conversion. This mechanism facilitated the achievement of satisfactory therapeutic outcomes in the RA treatment.
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ANALYTICAL CHEMISTRY
·
2026
·
IF 6.7
Kangbo Liu,Tingting Wang,Jingzhe Zang,Jing Zhou,Liang Zhao,Mengxin Zhang,Xueyi Qin,Xianwei Zhang,Peng Wang,Wancun Zhang
Accurate and in situ visualization of nickel ions (Ni2+) at inflammatory sites is crucial for early diagnosis, progression monitoring, and targeted intervention of nickel-induced pathological conditions. However, no reports have been reported on the in situ detection of Ni2+ at inflammatory sites. Herein, a programmable allosteric DNA nanomachine (ES-AP-tFNA) with dual-enzyme-mediated signal amplification for in vivo molecular imaging of Ni2+ at inflammatory sites was developed. ES-AP-tFNA can be sequentially cleaved by apurinic/apyrimidinic endonuclease 1 (APE1), which is highly expressed in inflammatory cells, and by the Ni2+-dependent DNAzyme, thereby enabling the in situ detection of Ni2+ within inflammatory microenvironments. First, the locked DNAzyme (S5) is released following the cleavage of the AP site in ES-AP-tFNA by APE1. Subsequently, the DNAzyme hybridizes with S6 through base complementarity and, in the presence of Ni2+, specifically cleaves riboadenosine (rA), resulting in the separation of the fluorophore and quencher. Furthermore, the liberated DNAzyme can repeatedly cleave rA sites on multiple S6 strands, enabling fluorescence signal amplification through a catalytic recycling mechanism. Experimental results demonstrate that ES-AP-tFNA exhibits high sensitivity (LOD: 0.08 U/mL for APE1, 0.25 μM for Ni2+) and specificity. Furthermore, ES-AP-tFNA can rapidly and specifically visualize Ni2+ in inflammatory cells at the implantation sites without causing significant organ toxicity in mouse models implanted with a nickel alloy.
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SCIENCE OF THE TOTAL ENVIRONMENT
·
2022
·
IF 10.753
Jian Zhu,Bin Yang,Li Peng,Jinwei Wu,Huimin Hao,Shuyan Lou
Perfluorinated compounds (PFCs) are useful man-made chemicals and serve as new emerging organic pollutants due to their environmental and health concerns. Chromatography-mass detection methods often need complex procedure and are also too expensive, so there is a critical demand to develop rapid, inexpensive, easy-to-operate and sensitive methods for PFCs detection. In this work, double fluorescent biosensors (‘DT sensor’ and ‘FT sensor’) have been designed to quantitatively detect long-chain perfluorinated compounds (PFCs), due to their strong hydrophobic interaction with DNA probe or lysozyme fiber. The ratio and rapid fluorescence responses offered more obvious signal changes, and high sensitivity with a limit of detection (LOD) of 0.16 μM (98.2 ppb) for perfluorododecanoic acid (PFDoA). For three PFCs with longer perfluoroalkyl chain (CF 2 ), increased detection sensitivity was achieved due to a stronger hydrophobicity. The fluorescent biosensors showed a good selectivity for long-chain PFCs and served as cross-reactive sensors to differentiate three different long-chain PFCs. The biosensors also had robust signal response in tap water or serum samples, and the LOD can be further lowered to pM (ppt) level after sample preconcentration.
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DALTON TRANSACTIONS
·
2022
·Vol. 52
·期 1
·147-158
·
IF 4.569
Linming Li,Ming Zhang,Dianlong Jia,Zhifang Liu,Ning Zhang,Bin Sun,Zhengping Wang,Min Liu,Qingpeng Wang
To develop new chemotherapeutics with anti-metastasis properties, a series of multi-specific niflumic acid (NFA) platinum(IV) complexes with DNA damage, inflammation inhibition, immunity activation, and angiogenesis suppression mechanisms were designed, synthesized and evaluated as novel antitumor agents. The dual NFA platinum(IV) complex with a cisplatin core showed promising antitumor activities both in vitro and in vivo with lower toxicity than platinum(II) drugs and displayed attractive anti-metastasis performance. It caused serious DNA damage and further elevated the expression of γ-H2AX. Furthermore, it promoted apoptosis by activating the mitochondrial apoptotic pathway and autophagy of tumor cells. Moreover, immune response in tumors was significantly improved by increasing CD3+, CD4+ and CD8+ T infiltrating cells. Subsequently, the pathway ERK/HIF-1α/VEGFA associated with angiogenesis was suppressed by the reduced inflammation and elevated immune response, and the density of microvessels marked by CD34 was significantly reduced in tumors. Accordingly, the multi-specific NFA platinum(IV) complexes have great potential to be developed as novel anti-proliferative and anti-metastatic drugs.
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MOLECULES
·
2023
·Vol. 28
·期 4
·1529
·
IF 4.6
Zhixiang Wang,Wentao Liu,Guohu Li,Jiacheng Wang,Bin Zhao,Peishan Huang,Wenjie Mei
Herein, two novel ruthenium(II) complexes coupled by erianinviaa flexible carbon chain, [Ru(phen)2(L1-(CH2)4-erianin)](ClO4)2(L1= 2-(2-(tri-fluoromethyphenyl))-imidazo [4,5f][1–10]phenanthroline (1) and [Ru(phen)2(L2-(CH2)4-eria)](ClO4)2(L2= 2-(4-(tri-fluoromethyphenyl))-imidazo [4,5f][1,10]phenanthroline (2), have been synthesized and investigated as a potential G-quadruplex(G4) DNA stabilizer. Both complexes, especially 2, can bind toc-mycG4 DNA with high affinity by electronic spectra, and the binding constant calculated for 1 and 2 is about 15.1 and 2.05 × 107M−1, respectively. This was further confirmed by the increase in fluorescence intensity for both complexes. Moreover, the positive band at 265 nm in the CD spectra ofc-mycG4 DNA decreased treated with 2, indicating that 2 may bind toc-mycG4 DNA through extern groove binding mode. Furthermore, fluorescence resonance energy transfer (FRET) assay indicated that the melting point ofc-mycG4 DNA treated with 1 and 2 increased 15.5 and 16.5 °C, respectively. Finally, molecular docking showed that 1 can bind toc-mycG4 DNA in the extern groove formed by base pairs G7–G9 and G22–A24, and 2 inserts into the small groove ofc-mycG4 DNA formed by base pairs T19–A24. In summary, these ruthenium(II) complexes, especially 2, can be developed as potentialc-mycG4 DNA stabilizers and will be exploited as potential anticancer agents in the future.Keywords:ruthenium(II) complexes;erianin;c-mycG-quadruplex DNA;stabilizer
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CHINESE JOURNAL OF STRUCTURAL CHEMISTRY
·
2022
·
IF 0.847
Hui-Chao GUAN,Xiao-Tong SONG,Gui-Bao LIU,Shu-Mei YUE
Complexes [Zn(pbm)(5-hip)3] ( 1 ), [Zn(pbm)(5-nip) 3 ] ( 2 ), [Mn(pbm)(H 3 btc) 2 (H 2 O)] ( 3 ) and [Mn(pbm)(5-nip) 3 ] ( 4 ), where H 2 HIPA = 5-hydroxyisophthalic acid, H 2 nip = 5-nitroisophthalic acid, H 3 btc = trimesic acid and pbm (pyridine benzene chelate material) = 2-(2-pyridyl)benzimidazole, were identified via single-crystal XRD analyses. 1, 2 and 4 pertain to the monoclinic space group C 2/ c , while 3 belongs to the triclinic space group P ① . The interplay of CT-DNA with those complexes was delineated using ultraviolet, fluorescence, and circular dichroism (CD) spectroscopy and viscosity measurements. Complexes 1, 2, 3 and 4 interact with CT-DNA in an electrostatic or grooving mode. We wish to offer a theory-wise foundation for developing anti-tumor medicines.
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MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 4
·1-12
·
IF 6.408
Zheng Delun,Yang Jianying,Zheng Zengyao,Peng Mingxuan,Ng Kwan-Ming,Chen Yaowen,Huang Linjia,Gao Wenhua
A metal–organic framework (MOF) of Cu-TPA (terephthalic acid) microsphere was prepared, followed by calcinating the MOF precursor of Cu-TPA/ZIF-8 mixture to obtain the CuO/ZnO. N-doped carbon dots (NCDs) were employed to combine the CuO/ZnO composite to form a tripartite heterostructured architecture of NCDs@CuO/ZnO, which led to a fierce enlargement of the photocurrent response. This was ascribed to the thinner-shell structure of the CuO microsphere and the fact that hollow ZnO particles could sharply promote the incidence intensity of visible light. The more porous defectiveness exposed on CuO/ZnO surface was in favor of rapidly infiltrating electrolyte ions. The p-n type CuO/ZnO composite with more contact interface could abridge the transfer distance of photo-induced electron (e −1 )/hole (h + ) pairs and repress their recombination availably. NCDs not only could boost electron transfer rate on the electrode interface but also successfully sensitized the CuO/ZnO composite, which resulted in high conversion efficiency of photon-to-electron. The probe DNA (S1) was firmly assembled on the modified ITO electrode surface (S1/NCDs@CuO/ZnO) through an amidation reaction. Under optimal conditions, the prepared DNA biosensor displayed a wide linear range of 1.0 × 10 −6 ~ 7.5 × 10 −1 nM and a low limit of detection (LOD) of 1.81 × 10 −7 nM for colitoxin DNA (S2) measure, which exhibited a better photoelectrochemistry (PEC) analysis performance than that obtained by differential pulse voltammetry techniques. The relative standard deviation (RSD) of the sensing platform for target DNA detection of 5.0 × 10 −2 nM was 6.3%. This proposed DNA biosensor also showed good selectivity, stability, and reproducibility, demonstrating that the well-designed and synthesized photoactive materials of NCDs@CuO/ZnO are promising candidates for PEC analysis. Graphical abstract
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MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Lina Wang,Xiangbiao Zeng,Li Zhang,Ying Yu,Bixia Lin,Yumin Wang,Manli Guo,Yujuan Cao
Herein, a novel filed-free electrochemical sensor based on inverted Y-type DNA conformation was developed for the ultrasensitive determination of tetracycline (TC). In this sensor, a ferrocene was immobilized on a DNA strand near the interface and showing a signal as reference. Meanwhile, a methylene blue (MB) was modified on a DNA vertical away from the interface, presenting a little signal. When the target TC attracted the aptamer, the DNA modifying MB was released, and then exchanged another DNA near the interface via toe-hold strand displacement effect. This caused an obvious enhancement of the MB signal, and achieved a sensitive response to the TC amount. In comparison to other DNA sensors based on field-confined assembling mode, this sensor exploited the transform of self-structure to make signal response, which avoids the steric hinerance effect and achieving field-free conversion. Meanwhile, the immobile ferrocene provided a reference proportion, reducing the influence of nonspecific signal on measurement accuracy. The followed assays demonstrated when the base-pairs on the straight side were 1.5-fold to that on the hypotenuse, the strand transferring efficiency and signal response were optimal. It exhibited a linearity of 1.0 × 10 -1 –1.0 × 10 3 nM for TC with a detection limit of 28.4 pM. In practical assay, the measured result was matched to that of the contrast method. Meanwhile, the recovery rates ranged from 98.0% to 103.0% with the RSD of 1.4%∼2.5%, respectively. Indicating an expectant application prospect of the proposed sensor in antibiotic monitoring.
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