INFLAMMATION
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2026
·
IF 5.4
Wang Zhengwei,Lin Jiani,Tan Guisen,Zhang Weimin,Chen Tao,Gao Ming,Liu Jiacheng,Wang Jianwei,Pan Tingzheng,Li Qingzhong,Zhang Hongtian,Shen Hongwei,He Xiaosheng,Liu Jiangang,Liu Zhuohao
The post-ischemic immune microenvironment critically balances neuroinflammation and tissue repair, making macrophage polarization a pivotal therapeutic target in cerebral infarction. Umbilical cord mesenchymal stromal cells (UCMSCs) modulate macrophage polarization primarily through paracrine signaling, while the key mediators and underlying mechanisms remain incompletely elucidated. Using proteomic array-based screening of co-culture supernatants, we identified soluble factors secreted by UCMSCs that regulate macrophage polarization. Stable soluble tumor necrosis factor receptor 1 (sTNFR1)-overexpressing (oe-sTNFR1) UCMSCs were generated via lentiviral transduction. The mechanisms by which sTNFR1 regulates macrophage polarization were investigated in vitro using immunoblotting, RT-qPCR, immunofluorescence, and flow cytometry. The therapeutic efficacy was evaluated in a rat model of middle cerebral artery occlusion (MCAO) via intracranial delivery of oe-sTNFR1 UCMSCs, with outcomes assessed via histological (Nissl and 2,3,5-triphenyltetrazolium chloride) staining, behavioral tests, and magnetic resonance imaging. To circumvent risks of genetic modification, a TNF-α-targeting nanobody (TNF-α Nb) was developed and tested in combination with wild-type UCMSCs. Proteomic analysis identified sTNFR1 as a key mediator secreted by UCMSCs for reprogramming macrophage polarization. oe-sTNFR1 UCMSCs enhanced macrophage chemotaxis and promoted reparative polarization in a paracrine-dependent manner. Mechanistically, UCMSC-derived sTNFR1 suppressed the NF-κB axis, which not only attenuated pro-inflammatory polarization but also derepressed SOCS3 signaling, thereby promoting the reparative phenotype. In MCAO rats, intracranial delivery of oe-sTNFR1 UCMSCs reshaped the post-ischemic brain microenvironment toward an anti-inflammatory and pro-repair state, improving neurological recovery through reduced infarct volume and improved functional outcomes. Furthermore, co-administration of the TNF-α Nb with wild-type UCMSCs enhanced a reparative myeloid phenotype and improved neurological outcomes compared to UCMSCs alone. This study establishes UCMSC-derived sTNFR1 as a key paracrine mediator of macrophage polarization via the sTNFR1/NF-κB/SOCS3 axis, facilitating functional recovery after ischemic stroke. The strategy combining a TNF-α Nb with UCMSCs offers a novel and clinically promising immunotherapeutic approach.
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高影响因子
MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 11
·729
·
IF 5.4
Wang Mengjun,Huang Rong,Yang Xinwen,Zeng Minghao,Wang Huizhen,Luo Xiaojun
A ratiometric fluorescence probe was developed by integrating a covalent organic framework (TP-BDB-COF) with N-doped quantum dots (N-CDs) encapsulated in zeolitic imidazolate framework-8 (N-CDs@ZIF-8), enabling highly sensitive and selective detection of hypochlorite (ClO⁻) in aqueous environments. The yellow-orange-emissive TP-BDB-COF, synthesized by the condensation of 2,4,6-triformylphloroglucinol (TP) and 3,3’-dimethyl-[1,1’-biphenyl]-4,4’-diamine (BDB), exhibited specific fluorescence quenching at 570 nm upon interaction with ClO⁻. The blue-emissive N-CDs@ZIF-8 served as a stable reference signal at 430 nm, enabling self-calibration via the ratiometric signal (F 430 /F 570 ). This dual-signal strategy significantly improved detection accuracy and reliability. This sensor exhibited a broad linear fluorometric response range of 0.05 to 200 µM for ClO⁻, with an exceptionally low detection limit of 22 nM. Moreover, its practical applicability was validated through analysis of real water samples, yielding satisfactory spike recoveries between 98.53% and 101.30%. This approach not only offers a robust method for monitoring ClO⁻ in complex matrices but also provides a versatile framework for constructing high-performance ratiometric sensors. Graphical abstract
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Journal of Neuroimmune Pharmacology
·
2024
·Vol. 19
·期 1
·1-17
·
IF 5.2
Lin Yun,Liu Tian,Chen Hong,Zeng Ming,Hu Shunwei,Yu Xiaoning,Chen Yonghua,Xia Chunmei,Wang Jin,Wang Jijiang
The occurrence of major asthma symptoms is largely attributed to airway vagal hypertonia, of which the central mechanisms remain unclear. This study tests the hypotheses that endothelin-1-mediated brainstem glial activation produces asthmatic airway vagal hypertonia via enhanced action of adenosine 5’-triphosphate on neuronal purinergic P2X4 receptors. A rat model of asthma was prepared using ovalbumin. Airway vagal tone was evaluated by the recurrent laryngeal discharge and plethysmographic measurement of pulmonary function. The changes in the brainstem were examined using ELISA, Western blot, luciferin-luciferase, quantitative reverse transcription-polymerase chain reaction, enzyme activity assay and immunofluorescent staining, respectively. The results showed that in the medulla of rats, endothelin receptor type B and P2X4 receptors were primarily expressed in astrocytes and neurons, respectively, and both of which, along with endothelin-1 content, were significantly increased after ovalbumin sensitization. Ovalbumin sensitization significantly increased recurrent laryngeal discharge, which was blocked by acute intracisternal injection of P2X4 receptor antagonist 5-BDBD, knockdown of brainstem P2X4 receptors, and chronic intraperitoneal injection of endothelin receptor type B antagonist BQ788, respectively. Ovalbumin sensitization activated microglia and astrocytes and significantly decreased ecto-5’-nucleotidase activity in the medulla, and all of which, together with the increase of medullary P2X4 receptor expression and decrease of pulmonary function, were reversed by chronic BQ788 treatment. These results demonstrated that in rats, allergic airway challenge activates both microglia and astrocytes in the medulla via enhanced endothelin-1/endothelin receptor type B signaling, which subsequently causes airway vagal hypertonia via augmented adenosine 5’-triphosphate/P2X4 receptor signaling in central neurons of airway vagal reflex. Graphical Abstract
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高影响因子
STRUCTURAL CHEMISTRY
·
2024
·
IF 2.1
Li Xi,Zhang Jian-Wei,Hu Xin-Cheng,Shi Xian-Lei,Wei Wei
In this work, by using a flexible tricarboxylate ligand 5-(4-carboxybenzyloxy) isophthalic acid (H 3 L), a novel pillar-layered Mn–organic framework, namely, [(CH 3 CH 2 ) 2 NH 2 ][Mn 3 (HL)L(A C ) 2 ] ( SQNU-23 , SQNU = Shangqiu Normal University), was synthesized by using a DEF/HAc solvent system. In SQNU-23 , each S-shaped Mn-O chain is interconnected by two bridging modes of organic ligand to form a 3D pillar-layered framework with 1D rectangular channels. The solid-state fluorescence of SQNU-23 exhibits a significant red shift of about 140 nm compared to that of organic ligand. Moreover, SQNU-23 as a luminescent thermometer exhibits good linear dependence in the wide temperature range (100 to 300 K).
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JOURNAL OF NANOBIOTECHNOLOGY
·
2025
·Vol. 23
·期 1
·1-27
·
IF 12.6
Peng Jiao,Liu Rongyan,Xu Junyi,Yao Yingjuan,Li Beibei,Chen Dengke,Chang Zhuangpeng,Zhao Rui,Feng Yanlin,Hou Ruigang,Lee Min,Zhang Xiao
Background Excessive generation of reactive oxygen species is a hallmark of the osteoporotic bone microenvironment, which leads to the damage of mitochondrial function and the deactivation of osteoblasts. Fruits and vegetables are rich sources of antioxidants, which play a key role in scavenging free radicals and maintaining the body’s homeostasis. Results Herein, we have developed a type of vesicle coming from carrots as nanoantioxidants to counteract oxidative stress and restore the vitality of osteoblasts for reversing osteoporosis. Nanovesicles are derived from carrot juice using a straightforward extrusion method, resulting in stable membrane structures containing various lipids and homologous active phytochemicals. Nanovesicles can maintain stable structures under normal physiological conditions (pH 7.4) and transform into aggregates in response to the acidic extracellular pH of osteoporosis (pH 4.0). As anticipated, nanovesicles can passively target and aggregate to osteoporotic bone, ease oxidative stress, restore mitochondrial function, promote osteoblastogenesis, and reduce bone loss in osteoporotic mice. Conclusions This work presents the first demonstration of nanovesicles derived from carrots as novel nanoantioxidants to realize the long-awaited osteogenesis, contributing to the exploration of a brand-new idea for reversing osteoporosis. Graphical
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2024
·
IF 0
Zhao Jingnan,Chen Junwen,Wang Qun,Xiong Renxuan,Ma Jun
As reported, the persistent toxic and harmful pollutant bisphenol A (BPA) from industrial emissions has been consistently found in aquatic environments inhabited by humans. Periodate (PI)-based advanced oxidation processes (AOPs) have been employed to degrade BPA, although activating PI proves more challenging compared to other oxidants. A novel nano iron metal catalyst, sulfided nanoscale iron-nickel bimetallic nanoparticle supported on biocarbon (S-(nFe 0 -Ni)/BC) was synthesized and utilized to activate PI for the removal of BPA. The morphology, structure, and composition of S-(nFe 0 -Ni)/BC were characterized using X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy-energy dispersive spectrometer (SEM–EDS), and fourier-transform infrared spectrum (FTIR). The catalyst demonstrates an excellent ability to activate PI, achieving a BPA removal efficacy of 86.4%, accompanied by a 33% reduction in total organic carbon (TOC) in the {S-(nFe 0 -Ni)/BC}/PI system. BPA degradation exhibited a significant change at the 5-min mark. In the first stage (0–5 min), nonlinear dynamic fitting research, combined with scavenging experiments, unveiled the competitive degradation of pollutants primarily driven by iodate radical ( \({{\mathrm{IO}}}_{3}^{\cdot }\) ), singlet oxygen \(\left({}^{1}{{\mathrm{O}}}_{2}\right)\) , and hydroxyl radical ( \(\cdot {\mathrm{OH}}\) ). The competitive dynamics aligned with the ExpAssoc model. The contribution rates of different active species during the second stage (5–120 min) were calculated. The contributions of main species to BPA removal follow the order of \({{\mathrm{IO}}}_{3}^{\cdot }\) > \({}^{1}{{\mathrm{O}}}_{2}\) > \(\cdot {\mathrm{OH}}\) throughout the entire process. The influence of various parameters, such as the dosage of S-(nFe 0 -Ni)/BC, initial PI concentration, BPA concentration, pH, temperature, and the presence of coexisting anions, was also examined. Finally, a plausible reaction mechanism in the system is proposed, suggesting that the {S-(nFe 0 -Ni)/BC}/PI system involves a heterogeneous synergistic reaction occurring primarily on the surface of S-(nFe 0 -Ni)/BC. Therefore, this study proposes a promising approach for PI-based AOPs to degrade organic pollutants, aiming to mitigate the irreversible harm caused by such pollutants to organisms and the environment.
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Nano Research
·
2024
·
IF 9.5
Tang Jianfu,Fan Xueqin,Huang Haozhou,Dong Xiaofei,Li Xueqi,Wang Peiru,Yin Ran,Xie Yanjun,Li Jian,Tan Gang,Pang Zhenqian,Gan Wentao
Using biodegradable material derived from renewable resources as petroleum-based plastics replacement is a promising way towards sustainable development. However, the insufficient mechanical properties and complex manufacturing process of bioplastics still need to be improved for high-quality food packages. Herein, we report a top-down strategy to transform natural wood into a clear wood packaging film through scalable delignification and polyvinyl alcohol (PVA) infiltration. The wood packaging film demonstrates a laminated structure with completely collapsed cell walls and PVA intertwined together after energy-saving air drying, resulting in high light transmittance with low haze, good mechanical performance, and high barrier performance for oxygen and water vapor. Molecular dynamics simulations reveal the underlying fracture mechanism between cellulose and PVA, which effectively enhances the Young’s modulus and strength of the wood packaging film. These findings contribute to the development of biodegradable and strong packaging materials, as well as other food-related applications, using sustainable wood.
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Nano Research
·
2024
·
IF 9.5
Wang Yong,Guo Wenting,Wang Bo,Zhou Ya,Hu Ping,Ren Jiangtao,Wang Erkang,Jin Yongdong
Nanofluidic devices have turned out to be exemplary systems for investigating fluidic transport properties in a highly restricted area, where the electrostatic interactions or chemical reactions between nanochannel and flowing species strongly dominate the ions and flow transport. Numerous nanofluidic devices have recently been explored to manipulate ion currents and construct electronic devices. Enlightened by electronic field effect transistors, utilizing the electric field effect of nanopore nanochannels has also been adopted to develop versatile nanofluidic devices. Here, we report a nanopore-based nanofluidic unijunction transistor composed of a conical glass nanopipette with the biomaterial polydopamine (PDA) coated at its outer surface. The asfabricated nanofluidic device exhibited negative differential resistance (NDR) and ion current oscillation (ICO) in ionic transport. The pre-doped copper ions in the PDA moved toward the tip as increasing the potential, having a robust shielding effect on the charge of the tip, thus affecting the surface charge density of the nanopore in the working zone. Finite element simulation based on a continuum model coupled with Stokes-Brinkman and Poisson-Nernst-Planck (PNP) equations revealed that the fluctuations in charge density remarkably affect the transport of ionic current in the nanofluidic device. The as-prepared nanofluidic semiconductor device was a ready-to-use equipment that required no additional external conditions. Our work provides a versatile and convenient way to construct nanofluidic electronic components; we believe by taking advantage of advanced surface modification methods, the oscillation frequency of the unijunction transistors could be controlled on demand, and more nanofluidic devices with resourceful functions would be exploited.
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Biochar
·
2024
·Vol. 6
·期 1
·1-14
·
IF 13.1
Chen Shanshuai,Tian Liu,Feng Xiaoqiang,Bao Huanyu,Wang Hongliang
Chitin and phytic acid are abundant sustainable resources commonly found in shrimp shells, crab shells, and various plants. However, they are underutilized, and their biomass value is frequently underestimated. To address this, the current study developed a strategy to synthesize efficient separator coatings for zinc-iodine (Zn-I) batteries using chitin and phytic acid as carbon sources (biochar). First, nanosheets of porous carbon doped with N and P (NP-PC), featuring multiple active sites, are synthesized through in situ carbonization of chitin and phytic acid. Subsequently, the synthesized materials are coated onto the surfaces of glass fiber (GF) separators, effectively addressing several persistent challenges associated with Zn-I batteries. Notably, the modified separator (NP-PC@GF) enhanced the rapid-discharge capability of the involved battery, effectively inhibited the diffusion of intermediate polyiodide species, stabilized the electrolyte interface, suppressed irreversible dendrite formation, and increased I utilization efficiency. Consequently, the battery demonstrated high electrochemical performance, with an initial capacity of 7.8 mAh cm –2 at 20 mA cm –2 , an initial specific capacity of 8.9 mAh cm –2 under high I loading, and a retention rate of 56% (5.1 mAh cm –2 ) at the 174th cycle. Overall, this research demonstrates the effective use of biochar for separator modification, facilitating the development of high-performance Zn-I batteries. Graphical
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Journal of Coatings Technology and Research
·
2024
·
IF 2.3
Mac Van Phuc,Do Minh Thanh,Nguyen Anh Hiep,Dao Phi Hung,Nguyen Thien Vuong,Pham Cong Nguyen,Nguyen Tuan Anh
This work explores how cenospheres (hollow microsphere) can provide a flame-retardant ability to the waterborne acrylic coating. For this purpose, the intumescent fire-retardant coating was prepared on the steel surface, from the acrylic emulsion polymer, flame-retardant additives (ammonium polyphosphate, melamine, pentaerythritol), and flame-retardant fillers (TiO 2 , Al(OH) 3 ). The experimental results showed that after 60-min fire resistance test under burning torch of 900–1000 °C, the backside temperature of coated steel was in the range of 200–250 °C. Addition of cenosphere into the acrylic polymer coating (at 2–10 wt%) enhanced its fire resistance performance by reducing the backside temperature of coated steels from 27 to 76 °C, as compared to the pure coating without FACs. Data from the furnace test showed that the presence of FACs in coating reduced its intumescent factor (from 14.9 to 33.5%) but produced the denser char layer with a better heat shielding ability. XRD analysis confirmed the interaction between cenosphere and coating matrices at high temperatures by forming the heat-stable compounds. TGA data demonstrated that increasing the content of FACs in coating increased its char weight residue at high temperatures and enhanced its thermal stability and fire resistance. Data from the mechanical test indicated that the presence of cenosphere in the acrylic polymer coating did not affect its hardness but decreased its adhesion to the steel surface (from 5.3 to 23.8%). Graphical abstract
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高影响因子
CHINESE JOURNAL OF POLYMER SCIENCE
·
2025
·
IF 4
Zhang Xiao-Hui,Zhao Ya-Jun,Wang Can,Tang Shan
Incorporating a low density of ester units into the backbone of polyethylene materials enhances their sustainability and recyclability while maintaining the main material properties of polyethylenes. Here we report a new way to access degradable polyethylene materials with a low content of in-chain ester units via mechanochemical backbone editing. Initially, ester groups are incorporated as side groups through catalytic copolymerization of ethylene with a cyclobutene-fused lactone monomer (CBL), yielding polyethylene materials with high molecular weights and adjustable thermomechanical properties. Subsequent solid-state ball-milling treatment selectively introduces side-chain ester groups into the main chain of the polyethylene materials via force-induced cycloreversion of the cyclobutane units. Under acidic conditions, hydrolysis of the resultant polyethylene materials with in-chain ester units facilitates further degradation into oligomers.
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JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
·
2024
·Vol. 333
·期 6
·2709-2722
·
IF 1.5
Wang Guohua,He Shan,Xiao Quanjin,Peng Kun,Tang Donghui,Dong Zhitao,Pan Menglu,Li Shiyou
This study investigates the effects of nitrate exposure on the indigenous consortium mediated remediation of uranium-contaminated groundwater. Intermittent experiments reveal that high-nitrate concentrations hinder uranium reduction, whereas using glycerophosphate as carbon source, microbe could immobilize uranium by generating biomineralized precipitates with high crystallinity, and these precipitates exhibited better stability at high-nitrate concentration compared to those formed at low-nitrate condition. We also elucidated the mechanism of uranium removal by microorganisms under different conditions by analyzing changes in solution chemistry, products formed and microbial community structures. This work extends the scope for treating uranium-contaminated groundwater and is important for achieving long-term treatment of uranium contamination.
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危险品化学品经营许可证（带存储）