JOURNAL OF CELLULAR BIOCHEMISTRY
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2018
·Vol. 120
·期 3
·4463-4471
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IF 2.959
Xingda Wu,Zhe Liu,Kejia Guo,Gang Ma,Shaowei Song
Objective This work aimed to study the activating transcription factor 2 or AMP-dependent transcription factor-2 (ATF-2) inhibition mediated gemcitabine sensitivity in human pancreatic cancer cells. Methods The protein and messenger RNA expressions of ATF-2 in 42 pancreatic cancer tissues and adjacent nontumorous tissues were detected. Kaplan-Meier survival analysis was performed based on the expression level of ATF-2 protein in tumor tissues. Then the pancreatic cancer cells were transduced with ATF-2-expressing lentivirus and small interfering RNAs (siRNAs) to investigate the effect of ATF-2 on pancreatic cancer cell invasion, epithelium to mesenchyme transition, apoptosis, and gemcitabine sensitivity. Results The expression of phosphorylated (p)-ATF-2 protein was upregulated in pancreatic cancer tissues compared with adjacent nontumorous tissues. Patients with relative higher p-ATF-2 level showed significantly lower survival time. Then we found that the transfection ATF-2 siRNA into BxPC3 cells inhibited cell proliferation, invasion, and epithelium to mesenchyme transition, but enhanced cell apoptosis. These changes could be enhanced by the additional administration of gemcitabine. In addition, we confirmed that the overexpression of ATF-2 in Panc-1 cells promoted cell invasion and epithelium to mesenchyme transition. Conclusion We concluded that inhibition-promoted ATF-2 expression was responsible for epithelium to mesenchyme transition and invasion of pancreatic cancer cells, while the inhibition of ATF-2 confers to gemcitabine sensitivity in human pancreatic cancer cells in vitro.
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ACS Catalysis
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2019
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IF 12.221
Yaqi Liu,Hong-Yan Ma,Da Lei,Lan-Lan Lou,Shuangxi Liu,Wuzong Zhou,Gui-Chang Wang,Kai Yu
The aerobic oxidation of alcohols and aldehydes over noble metal catalysts is a critical reaction for the catalytic conversion of carbohydrates into value-added chemicals from biomass. However, to fully understand the reaction mechanism, in particular, the role of O2 and the generated active oxygen species in these reactions is still a challenging target. In the present work, the sub-10 nm Pt nanocrystals with cubic (Pt-NCs), octahedral (Pt-NOs), and spherical (Pt-NSs) morphologies were synthesized and used as catalysts in aerobic oxidation of 5-hydroxymethyl-2-furfural (HMF). Through experimental and computational investigations, the facet-dependent O2 conversion pathway and catalytic oxidation performance were discussed. The molecular O2 tends to be dissociated to generate •OH on a Pt(100) surface but prefers to be reduced to •O2– on a Pt(111) surface. Moreover, Pt-NCs enclosed by the {100} facets exhibited significantly enhanced catalytic activity compared to Pt-NOs enclosed by the {111} facets and Pt-NSs, in particular, for the alcohol oxidation step. On the basis of the experimental data and density functional theory (DFT) calculations, an active oxygen species promoted dehydrogenation mechanism for aerobic oxidation of HMF was proposed. The dehydrogenation of the alcohol group is more favorable on the Pt(100) surface with assistance of •OH, which is the dominant active oxygen species on the Pt(100) surface. We anticipate that this work would provide new insight into the role of active oxygen species in aerobic oxidation of alcohols and aldehydes over noble metal catalysts.
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JOURNAL OF MOLECULAR STRUCTURE
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2019
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IF 2.12
Qiang Liu,Peijun Cai,Xiangyu Wan,Shuai Zhang,Kai Du,Qiang Yin
Two neodymium β -diketonates metal-organic complexes, Nd(TTA) 3 TPTZ ( 1 ) and Nd(BFA) 3 TPTZ ( 2 ), have been synthesized by using two β -diketone ligands 4,4,4-trifluoro-1-(2-thienyl)-1,3-butanedione (HTTA) and 4,4,4-trifluoro-1-phenyl-1,3-butanedione (HFBA), and a macrocyclic conjugate ligand, 2,4,6-tri(2-pyridyl)-1,3,5-triazine (TPTZ). The crystal structures of these two complexes were determined by single crystal X-ray diffraction. The X-ray diffraction analysis showed that each neodymium ion in the two complexes formed a Nine-Coordinated structure with six oxygen atoms of HTTA (or HBFA) ligands and three nitrogen atoms of TPTZ ligand by the characteristic of triclinic with P 1 ( — ) space group both. In the crystal structure of the two complexes, a very obvious aromatic donor-acceptor interaction can be observed between adjacent TPTZ ligand molecules, which play an important role in the formation of three-dimensional structures. The complexes were also characterized by infrared spectroscopy , elemental analysis, thermogravimetric and fluorescence spectra . Fluorescence analysis showed that the two complexes exhibited characteristic neodymium-centered emission due to the significant antenna effect of these ligands.
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SYNTHESIS-STUTTGART
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2019
·Vol. 51
·期 16
·3085-3090
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IF 2.867
Menglan Wang,Yudong Li,Qing-An Wu,Shuping Luo,Yuehui Li
Indole scaffold synthesis relies primarily on oxidative C–H amination of N-protected alkenylanilines for C–N intramolecular cyclization reactions. Herein, for the first time, without N-protection, various readily prepared 2-alkenylanilines were transformed into the desired indole products in good yields by using K2S2O8 as oxidant in the presence of catalytic amounts of FeF2. The K2S2O8/FeF2 system offers a direct and benign synthetic route to 3-arylindoles and it is applicable to a wide range of substituted indoles including drug intermediates.
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JOURNAL OF ALLOYS AND COMPOUNDS
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2018
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IF 3.779
Jing Li,Huilian Hao,Jianjun Wang,Wenyao Li,Wenzhong Shen
Nitrogen-enriched graphene coupled with nickel hydroxide nanosheets (Ni(OH) 2 /NG) hydrogel is successfully synthesized through a facile one-pot hydrothermal method . Comprehensive investigations reveal that the nitrogen atoms are successfully inserted into graphene and that the nickel hydroxide nanosheets (∼30–50 nm) are anchored on NG homogeneously. With the enhanced electroactivity caused by nitrogen doping and the synergy effect from Ni(OH) 2 nanosheets and NG, the Ni(OH) 2 /NG hydrogel electrode displays much better electrochemical properties than two individual electrodes. It features a specific capacitance as high as 896 F g −1 at 0.5 A g −1 and even 504 F g −1 at 12 A g −1 showing a high rate capability (56.3% retention with 24 times higher current density). An asymmetric supercapacitor device on the basic of Ni(OH) 2 /NG hydrogel and activated carbon (AC) was assembled and delivered a high energy density of 28.7 W h kg −1 at the power energy density of 0.36 kW kg −1 . Such results indicate that the Ni(OH) 2 /NG hydrogel could be considered as a promising candidate for electrochemical supercapacitors .
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JOURNAL OF HAZARDOUS MATERIALS
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2018
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IF 6.434
Kaiyu Jiang,Caihuan Huang,Rui Jiao,Weibin Bai,Jie Zheng,Shiyi Ou
Acrolein (ACR), glyoxal (GO), methylglyoxal (MGO), hydroxymethylfurfural (HMF), and malondialdehyde (MDA) are toxic contaminants for humans. This work aimed to investigate whether intake of proteins can mitigate their toxicity. Simulated gastrointestinal digestion of proteins from pork, chicken, milk powder and soy protein isolate eliminated amount of ACR, GO, MGO, HMF, and MDA. Among six amino acids, cysteine showed highest capacity for elimination of these toxic compounds through the formation of adducts; it reached the highest elimination capacity for GO, MGO, ACR, MDA, and HMF in 40 min at pH 2.0, and 20 min at pH 7.0. The formed adducts between cysteine and GO, MGO, or ACR showed much lower toxicity against Caco-2 cells. Incubation of the cells with 8 mM GO and MGO for 48 h decreased the cell viability to 16.1%, 16.9% respectively; while incubation of the same concentration of their adducts still kept the cell viability at 82.2% and 81.6% respectively. Cysteine showed much higher detoxifying capacity for ACR than GO and MGO, which can lower the toxicity of ACR toward Caco-2 cells by 80 times.
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PHYSICAL CHEMISTRY CHEMICAL PHYSICS
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2018
·Vol. 20
·期 38
·24796-24806
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IF 3.906
Chensheng Ma,Chris Tsz-Leung Chan,Ruth Chau-Ting Chan,Allen Ka-Wa Wong,Bowie Po-Yee Chung,Wai-Ming Kwok
Apart from being an analogue of the prototype for photoinduced intramolecular charge transfer (ICT), 2-ethylhexyl 4-dimethylaminobenzoate (EHDMABA) is also one of the earliest patented and most commonly used sunscreen components. There is, however, little documented information about the photophysics and factors affecting the photophysics of this molecule. Such information is of importance for both the understanding of the ICT reaction and assessing the underlying process of photoprotection, especially in view of the “sunscreen controversy” that has arisen from the contrasting in vivo vs. in vitro photobiological results on this and related UV filters. We report herein a femtosecond broadband time-resolved fluorescence (fs-TRF), complemented by transient absorption (fs-TA) to allow a full probe of the excited state cascades for EHDMABA and two of its derivatives in solvents of varied properties. The results provide direct evidence for a nearly solvent independent inner sphere ICT reaction occurring on the sub-picosecond time scale, and an ensuing solvent dictated deactivation of the ICT state. The ICT state in the aprotic solvent acetonitrile decayed solely through the intrinsic intersystem crossing (ISC) to produce a potentially harmful triplet excited state. In the protic solvent, the solvation and formation of ICT-induced solute–solvent hydrogen (H)-bonding opened the originally inaccessible internal conversion (IC) channel of the ICT state, leading to the rapid reformation of the ground state molecule with a unitary efficiency in the aqueous solution. This H-bonding-mediated IC restrained or eliminated the intrinsic ISC, providing a mechanism at the molecular level for the benign dissipation of the electronic excitation. The precise rate of IC was observed to vary with the alkoxy substituent and its efficiency was affected by the H-bonding capacity of the solvent. The findings of this work demonstrate the pivotal role of the microenvironment and the direct participation of solvent molecules through H-bonding in drastically altering the nonradiative dynamics and promoting or inhibiting photostability and photoprotection. This may assist in developing next-generation UV filters and help in improving formulation design for the optimal efficacy of sunscreen products. The pronounced H-bonding-induced fluorescence quenching and variation in the fluorescence wavelength imply that these molecules may also serve as a sensitive fluorescence probe for the H-bonding properties of the microenvironment.
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2018
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IF 3.974
Linbao Zhang,Ying Hu,Wei Sun,Si Chen,Xiaoping Jia,Wengui Cai
Di-(2-ethylhexyl) phthalate (DEHP) is currently the most frequently detected phthalic acid esters (PAEs) compounds and can induce diverse toxicities on aquatic organisms. To understand the molecular responses of fish to DEHP, we performed transcriptomic profiles in liver of tilapia ( Oreochromis niloticus ) which were exposed to environmental concentration of DEHP. A total of 30.10 Mb and 30.16 Mb clean reads were retrieved from the control and DEHP treated libraries, respectively. De-novo assembly of all the clean reads obtained 58,585 unigenes. After comparing the two libraries, 2814 and 1790 genes were identified as significantly increased and depressed, respectively. Gene ontology (GO) classification system and Kyoto Encyclopedia of Genes and Genomes (KEGG) database analysis demonstrated that DEHP significantly disturbed the expression level of genes associated with immunity, endocrine and reproductive system, lipid metabolism and so on. Quantitative real-time PCR was performed to validate the results of RNA-sequencing (RNA-seq) analysis. The resulting data provide new insights for exploring the molecular basis of tilapia response to DEHP exposure.
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JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
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2018
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IF 2.891
Zhi Zhu,Wenqian Fan,Zhi Liu,Yang Yu,Hongjun Dong,Pengwei Huo,Yongsheng Yan
The present study describes a metal-free biochar-based photocatalyst of g-C 3 N 4 -C, in presence of an abundant fade magnolia as carbon precursor via facial in-situ together growth method. As a consequence, the g-C 3 N 4 -C shows a better degradation efficiency to 2-Mercaptobenzothiazole (90%) than pure g-C 3 N 4 (49%) under the visible light. The improved photocatalytic performance of g-C 3 N 4 is attributed to the biochar can significantly against the g-C 3 N 4 reunion and stacking during high temperature treatment, because the biochar can act as the attachments that make the g-C 3 N 4 flat and no accumulation, which will further increase the active sites. Additionally, photo-electrochemical measurements proved the separation efficiency of electron-holes of g-C 3 N 4 is enhanced by introduced of biochar, due to its good electron transmission ability. Moreover, the ESR measurement and capture experiment further confirmed the active radicals on degradation of MBT are h + and O 2 − . This work may provide a promising approach for wastewater treatment by waste utilization of solid biomass and increasing the photocatalytic performance of g-C 3 N 4 .
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TETRAHEDRON
·
2014
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IF 2.817
Jing Yang,Shaohuan Sun,Biao Zhang,Juan Zhang,Yanlong Gu
2-Alkoxy-3,4-dihydropyrans were proved to be a class of powerful modular substrates to construct polyheterocycles by reacting with NH 2 -containing dinucleophiles, such as anthranilamide , 2-aminobenzenethiol, 1-(2-aminophenyl)pyrrole, and 2-( o -aminophenyl)indole in the presence of LiBr·H 2 O. A three-component reaction of isatoic anhydride , 2-alkoxy-3,4-dihydropyran and amine was also developed as well.
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CHEMICAL ENGINEERING JOURNAL
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2014
·
IF 4.058
Xianfei Zeng,Yang Zhou,Wenhui Ma,Shixing Wang,Keqiang Xie,Jijun Wu,Kuixian Wei
Di(2-picolyl)amine derivates functionalized inorganic/organic hybrid magnetic network was prepared for selective detection and removal of Zn (II). A significant visual color change from colorless to pink-red was observed under the irradiation of 365 nm UV lamp. The inorganic/organic hybrid Fe 3 O 4 @SiO 2 nanoparticles were characterized by TEM, XRD, VSM, FT-IR, and TGA techniques. The fluorescence photograph showed that di(2-picolyl)amine functionalized Fe 3 O 4 @SiO 2 nanoparticles were sensitive and selective to Zn 2+ in acetonitrile solution. The di(2-picolyl)amine functionalized Fe 3 O 4 @SiO 2 nanoparticles with high saturation magnetization can conveniently separate metal ions from aqueous solution. Fluorescence spectra showed that the intensity varied obviously with the concentration of Zn 2+ . The removal efficiency of Zn 2+ can reach as high as 97.1% and the saturated adsorption amount of Zn 2+ is 161.6 mg/g. Langmuir linear fitting can illustrate the well consistent with the adsorption results.
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ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
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2015
·
IF 2.084
Xian-Hua Chen,Li Ma,Yi-Xiang Hu,Dan-Xian Wang,Li Fang,Xue-Lai Li,Jin-Chuan Zhao,Hai-Rong Yu,Hua-Zhong Ying,Chen-Huan Yu
Tris (2-ethylhexyl) trimellitate (TOTM) is commonly used as an alternative plasticizer for medical devices. But very little information was available on its biological effects. In this study, we investigated toxicity effects of TOTM on hepatic differential gene expression analyzed by using high-throughput sequencing analysis for over-represented functions and phenotypically anchored to complementary histopathologic, and biochemical data in the liver of mice. Among 1668 candidate genes, 694 genes were up-regulated and 974 genes were down-regulated after TOTM exposure. Using Gene Ontology analysis, TOTM affected three processes: the cell cycle, metabolic process and oxidative activity. Furthermore, 11 key genes involved in the above processes were validated by real time PCR. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that these genes were involved in the cell cycle pathway, lipid metabolism and oxidative process. It revealed the transcriptome gene expression response to TOTM exposure in mouse, and these data could contribute to provide a clearer understanding of the molecular mechanisms of TOTM-induced hepatotoxicity in human.
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