Small Methods
·
2024
·
IF 10.7
Yijia Kang,Zhilan Qian,Haishuang Yu,Jian Lu,Qing Zhao,Xue Qiao,Min Ye,Xiangshan Zhou,Menghao Cai
Previous data established 4′-deoxyflavone glycosides (4′-DFGs) as important pharmaceutical components in the roots of rare medical plants like Scutellaria baicalensis Georgi. Extracting these compounds from plants involves land occupation and is environmentally unfriendly. Therefore, a modular (“plug-and-play”) yeast-consortium platform is developed to synthesize diverse 4′-DFGs de novo. By codon-optimizing glycosyltransferase genes from different organisms for Pichia pastoris , six site-specific glycosylation chassis are generated to be capable of biosynthesizing 18 different 4′-DFGs. Cellular factories showed increased 4′-DFG production (up to 18.6-fold) due to strengthened synthesis of UDP-sugar precursors and blocked hydrolysis of endogenous glycosides. Co-culturing upstream flavone-synthesis-module cells with downstream glycoside-transformation-module cells alleviated the toxicity of 4′-deoxyflavones and enabled high-level de novo synthesis of 4′-DFGs. Baicalin is produced at the highest level (1290.0 mg L −1 ) in a bioreactor by controlling the consortium through carbon-source shifting. These results provide a valuable reference for biosynthesizing plant-derived 4′-DFGs and other glycosides with potential therapeutic applications.
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高影响因子
JOM
·
2025
·
IF 2.3
Liang Yuanyuan,Dou Zhihe,Zhang Ting-an
Upgrading magnesium slag into value-added products provides a significant opportunity to address environmental waste pollution while promoting waste recycling. However, challenges such as the low catalytic activity of the resulting products and limited adsorption of macromolecular organic compounds at surface active sites remain critical engineering hurdles. In this paper, mesoporous g-C 3 N 4 /TiO 2 -MgAl 2 O 4 heterojunction photocatalysts were synthesized by a facile combustion method using magnesium slag, titanium dioxide, and carbamide as raw materials. Photocatalytic experiments on methylene blue solution under visible light irradiation at room temperature showed that the g-C 3 N 4 /TiO 2 -MgAl 2 O 4 heterojunction photocatalyst achieved a 25.6% higher degradation efficiency compared to traditional photocatalysts. This work provides a theoretical framework and practical experience for the value-added and low-cost preparation of photocatalytic materials derived from magnesium slag. Furthermore, a potential enhanced photocatalytic mechanism of g-C 3 N 4 /TiO 2 -MgAl 2 O 4 heterojunction photocatalysts is proposed.
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ADVANCED MATERIALS
·
2025
·
IF 26.8
Zihao Wu,Haoran Guo,Kexin Ren,Yasen Wang,Haotian Zhang,Fuhui Zhang,Lingfeng Liu,Guangye Li,Limin Qi
Traditional photoelectrochemical (PEC) systems struggle to simultaneously achieve high efficiency, high stability, and low cost. Replacing water oxidation with oxidation of organic molecules emerges as an attractive strategy to enhance the hydrogen production efficiency of PEC systems while generating value-added anodic products. Here, a PEC system utilizing a Mo, N co-doped BiVO 4 photoanode and NaClO 4 electrolyte for the glycerol oxidation reaction to approaching the theoretical limit of current doubling that enables a two-electron reaction to be driven by a single photon, is reported. While nitrogen doping optimizes the bulk charge separation/transport performance of Mo-doped BiVO 4 photoanodes, NaClO 4 as the supporting electrolyte further enhances the reaction kinetics and surface charge extraction efficiency. The optimized system reaches a record photocurrent density of 9.73 mA cm −2 at 1.23 V versus RHE and a maximum internal quantum efficiency of 182%. It predominantly produces C-C cleavage products, including glycolaldehyde and formaldehyde, and can maintain stable performance for over 500 h. DFT calculations reveal that glycerol can undergo adjacent hydroxyl bidentate chelation adsorption on the BiVO 4 surface. This system is applicable for the current doubling reaction of various polyhydroxy alcohols, providing a potential pathway for efficient valorization of platform molecules and effective recycling of waste plastics.
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高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2023
·Vol. 34
·期 8
·2309792
·
IF 19
Yan Ma,Zhuo Li,Shuhua Tu,Tianyu Zhu,Wanqing Xu,Min Chen,Lan Shi,Limin Wu
Highly sensitive electronic skin (e-skin) sensors with considerable operating range offer great possibilities for health care, human–-machine interfaces, and intelligent robotics. Although subtle microstructures have been used in recent years to improve the sensitivity and sensing range of e-skin sensors, the trade-off between these features remains a big challenge to overcome. Here, an asymmetric interlocked gradient elastic modulus (AIGM) structure is designed for the first time to provide sufficient deformation space to the traditional interlocked structure while ensuring high sensitivity, and the gradient elastic modulus further extends the sensing range of the AIGM structure-based sensors, which exhibit a maximum sensitivity of as high as 9.89 kPa −1 with an ultrawide working range that extends to above 530 kPa at the same time. As a demo, this sensor is integrated into an intelligent adjustable mouse platform for physiological signal monitoring, pressure feedback, and modulus adjustment, providing a viable alternative for hand disease prevention.
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JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
·
2016
·Vol. 16
·期 3
·2291-2295
·
IF 1.338
Liu Daofeng,Yu Zhibiao,Huang Yanmei,Wang Shuying,Wang Jingyun,Guo Qi,Xu Chaolian,Xia Shiqi,Lai Weihua
A competitive lateral flow assay for the rapid detection of Salmonella choleraesuis was developed. Immuno-magnetic nanobeads were produced by covalently coupling anti-Salmonella choleraesuis antibody to magnetic nanobeads. These immuno-magnetic nanobeads were used as visually
detected probes in the subsequent assay. Compared with the traditional sandwich assay, which is used for detecting macro-molecules, this new method was developed based on the competitive relationship between S. choleraesuis in the inspected sample and the outer membrane protein immobilized
on the T line. Thus, only one antibody was necessary in the new assay, whereas a pair of rigorously selected antibodies were required in the sandwich assay. The sensitivity of the competitive assay for S. choleraesuis was 1.2×107 cfu/mL. In addition, no cross reactions were
found in the 17 common non-Salmonella bacteria strains and in the 4 Salmonella strains of other serotypes. Thus, with satisfactory sensitivity and specificity, the assay can be applied for the rapid detection of pre-enriched culture that may contain S. choleraesuis.
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高影响因子
FISHERIES SCIENCE
·
2015
·Vol. 81
·期 2
·345-352
·
IF 0.878
Peng Tao,Wang Daojun,Yu Yingying,Liu Chaoliang,Zhu Baojian
Amylases catalyze the hydrolysis of starch material and play important roles in the regulation of many physiological processes. Here, we report an amylase gene denoted Pc - Amy in the crayfish Procambarus clarkii (Decapoda: Cambaridae). The Pc - Amy gene encodes a polypeptide of 514 amino acids, and the deduced amylase protein is highly homologous to those from crustaceans. Real-time quantitative reverse transcription PCR (qRT-PCR) analysis showed that Pc - Amy is highly expressed in hepatopancreas during the intermolt period. SDS-PAGE and western blot analysis demonstrated that a 58-kDa recombinant protein is successfully expressed in Escherichia coli cells. The expression of Pc - Amy was significantly downregulated in hepatopancreas at 48 h after ecdysteroid induction, as confirmed by qRT-PCR and western blot analyses. In addition, RNA interference with the ecdysteroid receptor had an impact on the expression of Pc - Amy . These results suggest that Pc - Amy is an ecdysteroid-responsive gene which plays a role in the developmental process of P. clarkii .
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SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Chenyang Mei,Luting Pan,Wenjin Xu,Hang Xu,Yuanyuan Zhang,Zhiying Li,Bin Dong,Xiangqing Ke,Colm McAlinden,Mei Yang,Qinmei Wang,Jinhai Huang
A sensitive, non-invasive and specific tear biomarker sensor for diabetic retinopathy (DR) could be of great significance in clinical practice. Vascular endothelial growth factor (VEGF) was targeted as a tear biomarker for DR. In principle, we used hybridization chain reaction (HCR) and CeO 2 nanoparticles as a cascade signal amplification strategy to construct a reusable aptasensor in search of VEGF. The report probes H 1 and H 2 modified with medium CeO 2 nanoparticles were added in and HCR reaction. The first analysis method for a quantitative detection of VEGF was obtained using CeO 2 nanoparticles to catalyze H 2 O 2 to generate electrical signals. The fabricated biosensor exhibited an ultrasensitive detection of VEGF down to 0.27 fg mL −1 , response range from 1 fg mL −1 to 0.1 ng mL −1 . Subsequently the aptasensor carried out a second repeated assembly to obtain a second signal by strand displacement reaction (SDR). The ratio of the former and latter signals was used to carry out a second analysis method for quantifying VEGF. The second analysis method had detection limits as low as 7.39 fg mL -1 , ranging from 10 fg mL −1 to 0.1 ng mL −1 . The aptasensor could maintain above 99 % signal in five days and shows the acceptable reproducibility. For a practical tear biomarker detection, the analysis of the tears was carried out simultaneously using ELISA and the aptasensor which showed excellent precision. This non-invasive screening method provides a potential tool for the early diagnosis and monitoring of DR and may improve screening success among high-risk patients with diabetes.
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ANALYTICA CHIMICA ACTA
·
2021
·
IF 6.558
Qi Dai,Xiaojuan Wang,Cai Liu,Zhenzhen Feng,Baosheng Ge,Xiqi Ma,Zhixiong Zhang,Jinyi Yu,Xiaoqiang Wang,Fang Huang
Melanoma, the skin cancer with the highest mortality rate, can be diagnosed at the early stage by detecting unique biomarkers. Over-expressed tyrosinase has been confirmed by dozens of clinical studies as an independent factor to evaluate the malignancy of melanoma. Using Enteromorpha Prolifera as the raw material, herein we develop a novel fluorescent probe , ECDY, which can sensitively detect the tyrosinase activity in different types of cells. More importantly, melanoma cells can be specifically distinguished through cell lysate measurements as well as the whole-cell imaging technique. Mechanically, the tyrosine groups on the surface of ECDY can be specifically recognized by tyrosinase and further converted into dopaquinone, which consequently causes the intramolecular fluorescence quenching of the probe through photoinduced electron transfer (PET). Tyrosinase can be detected within 20 min in the solution, and the detection limit is as low as 0.067 U mL −1 . For the in vitro demonstration, we evaluate the fluorescence decay of ECDY in response to the intracellular tyrosinase activity within the lysate of various cell lines, including non-cancerous, non-melanoma cancerous, and mouse melanoma ones. The experimental results verify that ECDY can accurately measure the apparent tyrosinase activity in different cell lines and detect melanoma cell lysate specifically. The confocal fluorescence imaging experiments further demonstrate that ECDY can distinguish melanoma cells from others significantly. We believe that ECDY provides a new strategy for the efficient detection of tyrosinase and melanoma cells, and is expected to apply as a clinical diagnosis platform.
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高影响因子
ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Xiangui Ma,Mengmeng Lv,Fangxin Du,Cunqi Wu,Baohua Lou,Abdallah M. Zeid,Guobao Xu
Some mustard compounds (mustards) are highly toxic chemical warfare agents. Some are explored as new anticancer drugs. Therefore, the fast, selective, and sensitive detection of mustards is extremely important for public security and cancer therapy. Mustards mostly target the N7 position on the guanine bases of DNA. The guanine-rich G-quadruplex DNA (G4) has been widely studied in the sensing area, and it was found that dimeric G4 (D-G4) could dramatically light up the fluorescence intensity of thioflavin T (ThT). Based on this, we used for the first time the D-G4 DNA as a selective probe for ultrasensitive fluorescence detection of nitrogen mustard (NM). When NM occupies the N7 on guanine, it can block the formation of the D-G4 structure due to the steric hindrance, and hence, it inhibits the combination of D-G4 with ThT, leading to a sharp decrease of fluorescence intensity. The proposed reaction mechanism is proved using ultraviolet–visible (UV–Vis) spectra, circular dichroism (CD) spectra, and polyacrylamide gel electrophoresis. Herein, the concentration of D-G4/ThT used is as low as 50 nM due to its highly fluorescent performance, enabling both high sensitivity and low cost. NM can be detected with a wide linear range from 10 to 2000 nM. The detection limit of NM reaches a surprisingly low concentration of 6 nM, which is 2 or 3 orders of magnitude lower than that of previously developed fluorescence methods for mustards and simulants.
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高影响因子
JOURNAL OF FORENSIC SCIENCES
·
2020
·Vol. 65
·期 3
·1016-1022
·
IF 1.441
Sizhe Huang,Rongshuai Wang,Bingbing Guo,Hongbin Ruan,Jinghong Ma,Liang Ren,Liang Liu
Methyl nitrite is suggested to cause methemoglobinemia by generating methemoglobin, which may be lethal when the methemoglobin concentration exceeds 70%. However, intoxication with methyl nitrite is seldom reported compared with that with other nitrites. Here, we present an industrial accident involving methyl nitrite inhalation during its synthesis process that resulted in three fatalities and one survivor. The autopsy revealed conspicuous blue-gray discoloration in various parts of the body, including the skin, airway mucosa, vessels, brain, heart, and among other areas. The toxicological tests on the deceased showed methemoglobin concentrations in the blood over the lethal level and increased nitrite ion levels in the blood, gastric contents, liver, and lung tissue compared with those in control samples. The cause of death was determined to be methemoglobinemia-induced hypoxia due to methyl nitrite inhalation. This report provides evidence that in methyl nitrite intoxication, exposure duration has a significant influence on the postmortem changes and likelihood of a fatal outcome may be related to the age of the victim. More attention is required regarding the industrial hazards of this substance.
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高影响因子
JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS
·
2020
·
IF 1.624
Xin Liu,Bo Chen,Yan Li,Yan Kong,Ming Gao,Lu Zhong Zhang,Ning Gu
Long-distance (⩾10 mm) arterial vascular defect injury was a massive challenge affecting human health. Compared with autologous transplantation, tissue-engineered scaffolds such as biocompatible silk fibroin (SF) scaffolds have been developed because they exhibit equivalent functional repair effects without adverse reactions. However, its mechanical strength and structural stability needed to be further improved to match the longer repair cycle of blood vessels while maintaining the original biological safety. Hence, we designed and prepared SF and hydrophobic polycaprolactone (PCL) composite microfibers by an improving electrospinning method. It was found that when the weight ratio of PCL to SF was 1: 1, a microfiber scaffold with high strength (6.16 N) and minimum degradability can be obtained. More importantly, compared with natural silk fibroin, the novel composite microfiber scaffolds can slightly inhibit cell infiltration and inflammation through co-culture with HUVECs in vitro and rabbit back transplantation in vivo. Furthermore, the fabricated scaffolds also demonstrated excellent structural stability in vivo because of the well-organized PCL doping in the structure. All these results indicated that the novel PCL/SF composite microfiber scaffolds were promising candidates for vascular tissue engineering applications.
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高影响因子
Journal of Materials Chemistry B
·
2022
·Vol. 10
·期 7
·1063-1076
·
IF 7.571
Lin Li,Haitao Peng,Yan Du,Heng Zheng,Aiping Yang,Guoyu Lv,Hong Li
Adhesives have attracted extensive attention in biomedical applications in recent years. However, the development of adhesives with strong adhesion in both dry and underwater conditions and antibacterial properties is still a challenge. Herein, a biomimetic adhesive (DP@TA/Gel) was developed based on the adhesion mechanism of mussel in water, from adhesion and solidification to avoiding excessive oxidization processes. DP@TA/Gel exhibited rapid strong nonspecific adhesiveness to diverse materials including wood (485 kPa) metal (507 kPa), plastic (74 kPa), and even fresh biological tissue (39 kPa) in dry conditions. Specially, owing to its biomimetic design, DP@TA/Gel could imitate the mussel adhesion mechanism underwater, endowing it with robust (38 kPa), highly repeatable (at least 15 times) and long-term (at least 120 h) stable adhesion even in underwater conditions. Remarkably, DP@TA/Gel also exhibited high adhesiveness in various water environments, including seawater, and a wide range of pH (3–11) and NaCl concentration (0.9–10%) solutions without any stimulus. In addition, DP@TA/Gel showed excellent biocompatibility and antibacterial properties. Thus, the DP@TA/Gel adhesive has appealing potential biomedical applications such as sutureless wound closure and as a tissue adhesive.
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高影响因子
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