SEPARATION AND PURIFICATION TECHNOLOGY
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2023
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IF 8.6
Yongli Shi,Jie Feng,Zhaoxin Zhang,Ningning Cao,Jiahao Li,Heping Li,Linji Li,Quanxian Hua,Qun Ma,Kai Zhang
Polypyrrole-based adsorbents have emerged in removing Cr(VI) from wastewater based on their reduction, chelation, electrostatic attraction and ion exchange. However, it is still a formidable challenge to remove metal cations simultaneously due to electrostatic repulsion. In fact, metal ions originating from industrial activities such as chemical manufacturing and metal electroplating are always complex and multifarious. Here, we report a EDTA-crosslinking-chitosan/polypyrrole composite (EDTA-CS/PPy) with multiple types of adsorption sites (carboxyl, hydroxyl, amino), successfully realizing simultaneous removal of Cr(VI) anions and other metal cations. The maximum adsorption capacities of Cr(VI), Cu(II), Pb(II) and Cd(II) reach up to 401.25, 59.03, 77.81 and 68.31 mg/g, respectively. The adsorption mechanism is attributed to coupling interactions of the reduction, chelation, electrostatic attraction and ion exchange between targeted metal ions and EDTA-CS/PPy, investigated by experiments and DFT theories. Notably, the adsorption capacity of EDTA-CS/PPy keeps stability after three adsorption–desorption cycles. These findings may promote potential applications of EDTA-CS/PPy adsorbents in highly efficient treatment of coexisting toxic metal ions from wastewater.
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COLLOID AND POLYMER SCIENCE
·
2023
·Vol. 301
·期 12
·1475-1486
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IF 2.4
Li Yinglu,Zhao Jing,Wang Yurui,Zhao Tianfu,Song Yanan,Liang Fei
Nanoscale metal organic frameworks (NMOFs) have become excellent drug carriers for the treatment of cancer or other diseases, due to the advantages of large surface area, high drug loading, tunable structure and easy functionalization, with potential application in the field of drug delivery. In this paper, UiO-66 NMOF was synthesized using terephthalic acid as organic ligand and zirconium chloride as metal salt, and then biocompatible chitosan (CS) and PEGylated chitosan (CS-g-mPEG)-decorated NMOFs as drug carriers were constructed by surface modification, using 5-FU as the drug model. The structures, size and morphology were characterized by IR, XRD, DLS, SEM and TEM respectively. The results showed that when the concentration was 0.0872 M, the molar ratio was 1:1 and the acetic acid amount was 2.9 mL, the average particle size of NMOFs was 145.60 nm and 171.73 nm, respectively. The drug loading capacity and encapsulation rate could reach 15.7% and 52.8%. The in vitro controlled drug release results indicated that the release rate accelerated with increasing temperature and decreasing initial amount, greatly reduced under acidic conditions, and the coating of CS and CS-g-mPEG on the surface of UiO-66 had the effect of controlling drug release, further improved the bioavailability of 5-FU. The decorated UiO-66 NMOFs show excellent biocompatibility as nanodrug carriers and can avoid rapid degradation under gastric acid, which are more suitable for oral drug delivery.
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COLLOIDS AND SURFACES B-BIOINTERFACES
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2023
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IF 5.8
Xiuqiong Chen,Lili Sun,Hongcai Wang,Shanshan Cao,Ting Shang,Huiqiong Yan,Qiang Lin
Alginate (Alg) hydrogels possess desirable advantages for application in tissue engineering; however, they are limited by their weak mechanical properties , poor chronical stability in phosphate buffered saline , and absence of mammalian cell recognition sites, severely restricting their biomedical applications. To overcome these limitations, we integrated Alg hydrogels with nano-silica (SiO 2 ) to produce nano-SiO 2 reinforced Alg–chitosan–gelatin nanocomposite hydrogels (Alg/SiO 2 –CHI–GA NCH) for biomedical purposes, utilizing Chitosan (CHI) and gelatin (GA) in an alternate electrostatic adsorption. Specifically, we investigated the regulatory and promotional effects of the nano-SiO 2 on the morphological structure, mechanical properties, thermal stability , rheological properties, swelling, biodegradability , biomineralization and cytocompatibility of the resultant Alg/SiO 2 -CHI-GA NCH. The experimental findings demonstrate that the constructed Alg/SiO 2 -CHI-GA NCH exhibited uniform morphology and a regular structure. Upon freeze–drying, the internal cross-sections of the NCH exhibited a honeycomb porous structure. Furthermore, the physicochemical properties and biological activities of the prepared Alg/SiO 2 –CHI–GA NCH were regulated to some extent by nano-SiO 2 content. Notably, nano-SiO 2 inclusion enhanced the attachment and viability of MG63 and MC3T3-E1 cells and induced three-dimensional cell growth in ALG/SiO 2 –CHI–GA NCH. Among the fabricated NCH, Alg/SiO 2 –CHI–GA NCH with 0.5% and 1.0% (w/v) nano-SiO 2 exhibited significant proliferative activity, which is attributable to their high porosity and uniform cell adhesion . Furthermore, the alkaline phosphatase activity in the cells gradually increased with increasing of nano-SiO 2 amount, indicating the favorable effect of nano-SiO 2 on the osteogenic differentiation of MG63 and MC3T3-E1 cells. Our study findings provide a comprehensive foundation for the structural- and property-related limitations of Alg hydrogels in biomedicine , thereby expanding their potential applications in tissue engineering.
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Frontiers in Pharmacology
·
2023
·
IF 5.6
Yulong Chen,Qingzhou Cheng,Shan Zeng,Site Lv
Background: Migraine is a common neurovascular disorder with typical throbbing and unilateral headaches, causing a considerable healthcare burden on the global economy. This research aims to prepare chitosan-alginate (CS-AL) nanoparticles (NPs) containing Foshousan oil (FSSO) and investigate its potential therapeutic effects on the treatment of migraine. Methods: FSSO-loaded CS-AL NPs were prepared by using the single emulsion solvent evaporation method. Lipopolysaccharide (LPS)-stimulated BV-2 cells and nitroglycerin (NTG)-induced migraine mice were further used to explore anti-migraine activities and potential mechanisms of this botanical drug. Results: FSSO-loaded CS-AL NPs (212.1 ± 5.2 nm, 45.1 ± 6.2 mV) had a well-defined spherical shape with prolonged drug release and good storage within 4 weeks. FSSO and FSSO-loaded CS-AL NPs (5, 10, and 15 μg/mL) showed anti-inflammatory activities in LPS-treated BV-2 cells via reducing the levels of pro-inflammatory cytokines such as tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6), and nitric oxide (NO), but elevating interleukin-10 (IL-10) expressions. Moreover, FSSO-loaded CS-AL NPs (52 and 104 mg/kg) raised pain thresholds against the hot stimulus and decreased acetic acid-induced writhing frequency and foot-licking duration in NTG-induced migraine mice. Compared with the model group, calcitonin gene-related peptide (CGRP) and NO levels were downregulated, but 5-hydroxytryptamine (5-HT) and endothelin (ET) levels were upregulated along with rebalanced ET/NO ratio, and vasomotor dysfunction was alleviated by promoting cerebral blood flow (CBF) in the FSSO-loaded CS-AL NPs (104 mg/kg) group. Conclusion: FSSO-loaded CS-AL NPs could attenuate migraine via inhibiting neuroinflammation in LPS-stimulated BV-2 cells and regulating vasoactive substances in NTG-induced migraine mice. These findings suggest that the FSS formula may be exploited as new phytotherapy for treating migraine.
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POLYMER
·
2023
·
IF 4.6
Hongnan Zhang,Mengting Cui,Tingting Zhang,Xiaohong Qin
Traditional textiles are conductive to breed germs that might cause infection or unpleasant odor, especially in public hygiene products. Moreover, biosafety, environmental protection and cost are crucial considerations for antimicrobial materials. Herein, we developed a biocompatible microcapsules/nanofibrous membrane using eco-friendly and facile strategy. The microcapsules loaded with the Epigallocatechin and gallate (EGCG) were synthesized through an ion-crosslinking reaction between chitosan (CS) and sodium tripolyphosphate (TPP). Additionally, the addition of octadecyl trimethyl ammonium chloride (STAC) could further enhance the antibacterial activity . Following that, the microcapsules were combined with the polycaprolactone (PCL) nanofibers . The resultant composite nanofibrous membrane exhibits a slow release effect, an enjoyable deodorant activity, and strengthened antibacterial activity through contact-killing and biocide-releasing. Besides, the membrane behaved a low degradation rate , without compromising its mechanical and hydrophilic properties after a 20-day degradation. These findings provided a biocompatible and practical approach for antimicrobial and odor-proof material for hygienic and sanitary products.
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Carbon Letters
·
2023
·Vol. 33
·期 6
·1661-1667
·
IF 4.5
Tian Tingyu,Cai Yuping,Yang Shimao,Guo Yanwei,Zhou Wei
In this study, we successfully grafted chitosan (CS) onto multi-walled carbon nanotubes (MWCNTs) to enhance their properties and potential applications in the biomedical field. FTIR spectroscopy confirmed the successful covalent bonding of CS onto MWCNTs, indicated by the new absorption peak of the amide bond (–CONH–). Thermal analysis showed that the modified MWCNTs (MWCNT-CS) had significant weight loss around 260 °C, suggesting the decomposition of hydroxypropyl chitosan, and confirming its presence in the nanocomposite. SEM images revealed that CS grafting improved the dispersibility of MWCNTs, a property crucial for their use as nanofillers in polymers. Moreover, the micro-tensile bond strength of dentin surface increased with increasing MWCNT-CS concentrations, indicating the potential of MWCNT-CS as a pretreatment for dentin bonding. After simulated aging, the bond strength remained significantly higher for MWCNT-CS groups compared to those without pretreatment. In biocompatibility assessment using the MTT assay, MWCNT-CS showed higher cell viability than MWCNT, suggesting improved biocompatibility after CS modification. The results of this study suggest that CS-modified MWCNTs could be promising materials for applications in dentin bonding, dentin mineralization, bone scaffolding, implants, and drug delivery systems.
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Scientific Reports
·
2023
·Vol. 13
·期 1
·1-15
·
IF 4.6
Zheng Xiaoran,Gao Min,Wu Liangquan,Lu Xin,Lin Qiuqi,Zhong Hai,Lu Yingfei,Zhang Yunlei,Zhang Xiuwei
Pseudomonas aeruginosa ( P. aeruginosa ) infections present a grave threat to immunocompromised individuals, particularly those with cystic fibrosis due to the development of bacterial biofilms. In this study, we engineered self-assembling chitosan-ceftazidime nanoparticles (CSCE) capable of effectively penetrating biofilms and eradicating P. aeruginos a. The CSCE nanoparticles were synthesized through ionic cross-linking, combining negatively charged ceftazidime with positively charged chitosan, resulting in uniform nanoparticles measuring approximately 40 nm in diameter, exhibiting high dispersity and excellent biocompatibility. Remarkably, these nanoparticles exhibited significant inhibition of P. aeruginosa growth, reduced pyocyanin production, and diminished biofilm formation, achieving a maximum inhibition rate of 22.44%. Furthermore, in vivo investigations demonstrated enhanced survival in mice with abdominal P. aeruginosa infection following treatment with CSCE nanoparticles, accompanied by reduced levels of inflammatory cytokines Interleukin-6 (125.79 ± 18.63 pg/mL), Interleukin-17 (125.67 ± 5.94 pg/mL), and Tumor Necrosis Factor-α (135.4 ± 11.77 pg/mL). Critically, mice treated with CSCE nanoparticles showed no presence of bacteria in the bloodstream following intraperitoneal P. aeruginosa infection. Collectively, our findings highlight the potential of these synthesized nanoparticles as effective agents against P. aeruginosa infections.
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EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
·
2023
·
IF 4.6
Yingqiao Li,Zhiru Zou,Jinyu An,Xiaoyao Liu,Qian Wu,Junpeng Sun,Xiaobang Liu,Jiaqun Du,Ying Xiong,Chao Wu,Xifan Mei,He Tian
Spinal cord injury (SCI) is a central system disease with a high rate of disability. Pathological changes such as ischemia and hypoxia of local tissues, oxidative stress and apoptosis could lead to limb pain, paralysis and even life-threatening. It was reported that catalase (CAT) was the main antioxidant in organisms, which could remove reactive oxygen species (ROS) and release oxygen (O 2 ). However, the efficacy of the drug is largely limited due to its poor stability, low bioavailability and inability to cross the blood spinal cord barrier (BSCB). Therefore, in this study, we prepared folic acid-functionalized chitosan nanoparticles to deliver CAT (FA-CSN CAT) for solving this problem. In vivo small animal imaging results showed that FA-CSN could carry CAT across the BSCB and target to the inflammatory site. In addition, Immunofluorescence, ROS assay and JC-1 probe were used to detect the therapeutic effect of FA-CSN CAT in vitro and in vivo. The results showed that FA-CSN CAT could alleviate the hypoxic environment at the injured site and remove ROS, thereby inhibiting oxidative stress and protecting neurons, which may provide a new idea for clinical medication of SCI.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Jingmin Cui,Yan Sun,Linqing Wang,Wenqiang Tan,Zhanyong Guo
In this study, nine chitosan derivatives containing aromatic five-membered heterocycles were prepared and the effects of different grafting methods on the biological activities of chitosan derivatives were investigated. The structures of all the compounds were characterized by Fourier Transform Infrared (FT-IR) spectroscopy and Nuclear Magnetic Resonance (NMR) spectroscopy, while the antioxidant, antifungal and antibacterial activities of the chitosan derivatives were tested. The experimental data suggested that the chitosan derivatives had outstanding inhibitory ability against Fusarium graminearum , Fusarium oxysporum f . sp . cucumbrum , Staphylococcus aureus and Escherichia coli . At the same time, some of the compounds showed strong scavenging ability against DPPH radical and superoxide radical. Cytotoxicity experiments have demonstrated that some chitosan derivatives are non-toxic to L929 cells. More importantly, compared to chitosan, these chitosan derivatives have good water solubility and can be used as potential polymers for antifungal and antibacterial biomaterials in agriculture.
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JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
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2022
·
IF 5.062
Dickson Pius Wande,Yue Qiu,Shijie Chen,Lan Yao,Yanghui Xu,Jing Yao,Hui Xiong
Oxaliplatin (OXA) and rapamycin (RAPA) combination therapy have shown the advantages of synergistically inhibiting the growth of cancer cells by enhancing apoptosis in cancerous cell lines. However, drug resistance and the lack of an orally administered formulation which exhibited the best clinical dosing compliance limited the clinical application of this regimen. Here, we report a nanogel-polymersomes drug delivery system (i.e.TMOR-CDAN) based on modified chitosan, chitosan diacetate (CDA), methoxy-poly-(ethylene glycol)-b-poly (lactide) (MPP), and D-alpha-tocopheryl polyethylene glycol succinate (TPGS) with permeation-glycoprotein (P-gp) inhibition capability for enhancing the oral chemotherapy efficacy of erroneously soluble RAPA and its synergistic chemotherapy with OXA. Hydrophilic OXA and hydrophobic RAPA were co-loaded using MPP and TPGS-based polymer capsules (i.e. TMOR), which were further coated by chitosan-modified gel to form TMOR-CDAN. The TMOR-CDAN displayed higher stability in both acidic and basic pH than free OXA and RAPA and could withstand the harsh gastric environmental conditions, preventing the burst drugs release while releasing the co-loaded drugs in a sustained and controlled manner. The dual-drug-loaded TMOR-CDAN provides a bifunctional platform for synergistic tumor therapy. Compared with the single drug OXA, RAPA, or the OXA and RAPA mixtures (OR), the TMOR-CDAN showed more outstanding in vitro cytotoxicity and in vivo antitumor chemotherapeutic efficacy, endowing the TMOR-CDAN with a prodigious potential for futuristic cancer chemotherapy .
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MEAT SCIENCE
·
2022
·
IF 7.077
Huiyun Zhang,Xinling Li,Huaibin Kang,Xinyan Peng
The potential of chitosan nanoparticles (CHNPs) for improving the quality stability of pork patties under freeze-thaw (F-T) cycles was studied. Chitosan nanoparticles were successfully produced by the ionotropic gelation method. Pork patties were incorporated with 0%, 0.5% chitosan, 1% chitosan, 0.5% CHNPs, and 1% CHNPs and then subjected to repeated F-T cycles. Results showed that CHNPs were able to inhibit lipid and protein oxidation with a significant decrease in the lipid malondialdehyde and protein carbonyl formation in the patties after seven F-T cycles. Low-field nuclear magnetic resonance (LF-NMR) results indicated that CHNPs effectively restrained water mobility of samples. In addition, CHNPs exhibited a distinguishable improvement in the textural properties and water holding capacity in the stored patties during F-T cycles. Thus, CHNPs could be used as a natural ingredient to protect frozen patties from quality degradation caused by temperature fluctuations.
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CARBOHYDRATE POLYMERS
·
2022
·
IF 10.723
Weiguang Zhao,Shiyi Cao,Haoxin Cai,Yang Wu,Qi Pan,Heng Lin,Jun Fang,Yinyan He,Hongbing Deng,Zhihong Liu
Reconstruction of multilayered and functional smooth muscle tissues remains a challenge nowadays. Cryogels possess great advantages in three-dimensional tissue regeneration owing to the interconnected macroporous structure, but their applications have been hindered because of limited mechanical properties. Inspired by the natural extracellular matrix, cellulose acetate electrospun nanofibers (NFs) were incorporated to chitosan/silk fibroin (CS/SF) cryogel scaffolds to address this problem in this work. Compared with pure CS/SF scaffolds, CS/SF/NFs composite scaffolds showed roughened surface and enlarged pore size (216.2 ± 65.3 μm vs. 263.1 ± 75.7 μm) allowing for cell adhesion and proliferation. Incorporation of nanofibers upgraded the mechanical properties of scaffolds with a six-fold increase in compressive modulus. The proliferation and infiltration of smooth muscle cells (SMCs) were remarkably improved with the existence of nanofibers. Besides, SMCs cultured on CS/SF/NFs scaffolds displayed higher expression of contraction-related genes, verifying their potential for smooth muscle tissue engineering.
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