ANALYTICAL CHEMISTRY
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2017
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IF 6.32
Yulong Wang,Zhenhe Su,Limin Wang,Jinbo Dong,Juanjuan Xue,Jiao Yu,Yuan Wang,Xiude Hua,Minghua Wang,Cunzheng Zhang,Fengquan Liu
We have developed a rapid and ultrasensitive surface-enhanced Raman scattering (SERS) assay for Cu2+ detection using the multiple antibiotic resistance regulator (MarR) as specific bridging molecules in a SERS hot-spot model. In the assay, Cu2+ induces formation of MarR tetramers, which provide Au nanoparticle (NP)–AuNP bridges, resulting in the formation of SERS hot spots. 4-Mercaptobenzoic acid (4-MBA) was used as a Raman reporter. The addition of Cu2+ increased the Raman intensity of 4-MBA. Use of a dual hot-spot signal-amplification strategy based on AuNP–AgNP heterodimers combined through antigen–antibody reactions increased the sensitivity of the sensing platform by 50-fold. The proposed method gave a linear response for Cu2+ detection in the range of 0.5–1000 nM, with a detection limit of 0.18 nM, which is 5 orders of magnitude lower than the U.S. Environmental Protection Agency limit for Cu2+ in drinking water (20 μM). In addition, all analyses can be completed in less than 15 min. The high sensitivity, high specificity, and rapid detection capacity of the SERS assay therefore provide a combined advantage over current assays.
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MICROCHEMICAL JOURNAL
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2024
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IF 4.9
Feina Niu,Panpan Jiang,Lei Wang,Yawen Gao,Hong Zhu,Nan Gao,Zhiwei Cai,Hanping He,Yunbin He,Gang Chang
Raman studies have consistently emphasized the precise detection of target analytes by strategically manipulating the morphology and structure of noble metal nanomaterials. In this study, the nanorods with Ag shell and Au nanobipyramid core (Au NBPs@Ag) were designed and prepared through the weting chemcial method. The length of the nanorods can be adjusted from 131 ± 8 nm to 165 ± 23 nm by controlling the amount of Ag precursor, while keeping their width constant. According to the size of Au NBPs@Ag nanorods, surface-enhanced Raman scattering (SERS) performance would change significantly, and the optimal signal response was achieved when the aspect ratio of Au NBPs@Ag nanorods was 4.43. This can be due to the synergistic effect of gold core and silver shell changed by nanorods morphology, which further affected SERS performance. Moreover, Au NBPs@Ag modified polydimethylsiloxane (PDMS) flexible substrate was developed through interface self-assembly technology, allowing for sampling on irregular surfaces. The flexible SERS substrate was successfully applied to detect malachite green in contaminated river bass with wide linear range from 0.1 nM to 10 μM and detection limit of 0.1 nM. These results indicated that Au NBPs@Ag nanorods SERS substrate had great potential in the rapid trace detection of environmental detection and food safety.
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MICROCHEMICAL JOURNAL
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2025
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IF 5.1
Jiamiao Wang,Jurong Lv,Zheyu Shen,Yan Liu,Xia Gao,Qiang Chen,Pei Liang
Organotin compounds (OTCs) are a hazardous substance that threatens the environment and human health. This study aims to detect organotin effectively. The first step was to compute the Raman spectra of organotin substances using density functional theory (DFT). This was followed by the collection of experimental data for the corresponding spectra. Finally, the gold and silver nanoparticles were prepared as substrates for surface-enhanced Raman scattering (SERS) detection. The SERS spectra were used to extract Raman characteristics that exclusively belong to the same type of metabolites. Additionally, a correspondence was established between these Raman characteristics and their respective vibration modes. The database contains the original SERS spectra and mapping data of the mentioned OTCs, along with the detailed characteristics of their Raman peaks, particularly their unique ones. The results indicate that silver nanoparticles are an effective substrate for SERS detection, providing a sensitive technique for trace detection of organotin compounds.
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BIOSENSORS & BIOELECTRONICS
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2024
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IF 10.7
Han Jiang,Xiaofan Zhu,Jingran Jiao,Chao Yan,Kaiyong Liu,Wei Chen,Panzhu Qin
Accurate detection of multiple pathogens in the early stages of infection is critical for guiding treatment and saving patients' lives, but current methods are still challenged by low sensitivity, poor robustness and long turnaround times. Here, we report a CRISPR/dCas9-based hotspot self-assembling surface-enhanced Raman scattering (SERS) biosensor (called dCasSERS) and its integration with a smartphone to address these challenges. In this design, bacterial DNA was pre-amplified by loop-mediated isothermal amplification (LAMP), and the repeat sequences of the amplicons were recognized by CRISPR/dCas9, providing abundant sites for the assembly of gold nanoparticles (AuNPs) and forming numerous hotspots for SERS analysis. Using AuNPs labeled with different Raman molecules as reporters, the presence of Staphylococcus aureus ( S. aureus ), Pseudomonas aeruginosa ( P. aeruginosa ) and Escherichia coli O157:H7 ( E. coli O157:H7) could be dexterously converted into distinguishable SERS signals. CRISPR/dCas9-based amplicon-specific recognition and SERS hotspot self-assembly improved the specificity and sensitivity, enabling the biosensor to simultaneously detect three target pathogens down to 1 CFU/mL without any cross-reactivity. By introducing a rapid extraction procedure and a smartphone-integrated handheld Raman spectrometer, rapid on-site analysis of multiple pathogens could be achieved in less than 50 min. The accuracy and robustness of the biosensor were demonstrated by 500 real urine specimens. This study displays a new paradigm for CRISPR/Cas-based self-assembly of SERS hotspots and provides insight into the future development of pathogen screening tools.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2025
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IF 4.6
Yansheng Liu,Mengqi Wang,Guofu Wang,Xiaobo Jia,Jin Zhou,Hongqi Li,Haixin Chang,Zhaoxu Li
This work reported a surface-enhanced Raman spectroscopy (SERS)-based microfluidic chip that detects mercury ions (Hg 2+ ) in water with high sensitivity and good reproducibility. Silver nanoparticles (AgNPs) are easily fabricated on a Si substrate using a thin, thermally treated Ag film. Cy3 functionalized single-stranded DNA (Cy3-ssDNA) serves as the probe, which is immobilized on the AgNPs (Cy3-ssDNA/AgNPs), generating a SERS-based sensing surface. Due to the strong interaction between thymine (T) bases and Hg 2+ , in the presence of these ions, the ssDNA forms a T-Hg 2+ -T hairpin structure, which enhances the SERS signal. This method exhibits a limit of detection (LOD) of 1 × 10 −13 M. Furthermore, the proposed SERS chip demonstrates exceptional selectivity for mercury ions, as well as good reusability. The reusability of the SERS microfluidic chip was evaluated using the L-cysteine, which has a stronger affinity than T for Hg 2+ . By applying L-cysteine, the chip can be reused 10 times for the detection of Hg 2+ at concentrations as low as 1 × 10 −8 M. The method proposed in this study shows good sensitivity and holds good potential for application in the detection of Hg 2+ .
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APPLIED SURFACE SCIENCE
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2025
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IF 6.9
Mingyin Zhang,Qifan Zhang,Dongjie Zhang,Zhenhuan Tian,Qiang Li,Feng Yun
A versatile surface-enhanced Raman spectroscopy (SERS) substrate capable of responding to excitation by both visible and ultraviolet (UV) lasers is challenging. Herein, an ultrasensitive and long-term stable SERS substrate suitable for both visible and UV excitation is realized by a structure of hexagonal boron nitride (hBN) nanosheet film-veiled Ag nanoparticles (hBN/Ag substrate). Utilizing the hBN/Ag substrate, the detection limit for crystal violet (CV) under a 532 nm laser is remarkably low at 1.0 × 10 -15 M; adenine, a biomarker, can be detected under UV excitation (a 325 nm laser) with a low limit of 1.0 × 10 -7 M. The hBN/Ag substrate provides abundant electromagnetic (EM) hotspots and multiple charge transfer paths under both 532 nm and 325 nm lasers. This cooperation of EM and chemical enhancement confers ultrasensitive and versatile SERS performance to the substrate. Furthermore, the hBN/Ag substrate demonstrates excellent reproducibility and stability even after 6 months in air, maintaining the SERS intensity of CV at over 86 % of that on the freshly prepared substrate, with a relative standard deviation of 6.57 %. These results indicate that the hBN/Ag substrate can be employed in visible and UV SERS for widespread and practical applications.
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FOOD CHEMISTRY
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2025
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IF 9.8
Yunpeng Shao,Yue Niu,Wenlong Deng,Zicheng Zhang,Ruien Yu,Linyu Mei
In this study, we prepared large-area polydimethylsiloxane (PDMS) 3D flexible films deposited with silver nanoparticles (Ag NPs) by assembling Ag NPs on ordered micron-column (MC) arrays fabricated using SU-8 colloidal lithography (Ag NP@SU-8-MC). The large-scale, highly ordered 3D MC array flexible film offers numerous adsorption sites for densely packed Ag NPs. High-density sub-nanometer gaps between Ag NPs create spatial hot spots, enhancing the electromagnetic field. The results indicate that this flexible substrate achieves a detection limit of 10 −15 M for rhodamine 6G (R6G), with a relative standard deviation of less than 8 %, demonstrating excellent SERS sensitivity and uniformity. Furthermore, leveraging its high viscosity and flexibility, the ‘pasting-extraction’ method was employed to detect carbendazim and thiram on the surfaces of tomatoes and apples, achieving detection limits of 10 −10 M and 10 −11 M, respectively. Overall, this work presents a practical approach for preparing flexible and sensitive SERS substrates for food safety applications.
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JOURNAL OF ALLOYS AND COMPOUNDS
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2024
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IF 5.8
Xiaohan Wang,Jingru Wang,Wenshi Zhao,Rui Guo,Sicheng Cui,Jie Huang,Jiaxin Lu,He Liu,Yang Liu
Tetracycline (TC) is widely distributed in environment and a significant environmental pollutant. It has been challenging to rapidly identify and effectively remove TC contaminants. This work produced a multifunctional Fe 3 O 4 -Cu 2 O-Au-MIPs nanocomposite (FCAu-MIPs NCs) based on molecular imprinting technology for selective SERS detection and photocatalytic degradation of TC. We synthesized FCAu NCs with various Au contents and analyzed the relationship between Au content and SERS performance using finite-difference time-domain (FDTD) technique. Exceptional SERS performance could be attributed to the dense distribution of multiple hotspots on the SERS substrate. The photocatalytic mechanism of FCAu-MIPs NCs towards TC was also discussed. The combination of the narrow-bandgap semiconductor Cu 2 O and noble metal Au with localized surface plasmon resonance (LSPR) effect facilitated efficient light absorption and enhanced charge carrier transfer efficiency. Both SERS detection limits as low as 10 −9 M and selective photocatalytic degradation of TC in soil were achieved. Furthermore, FCAu-MIPs NCs were successfully recovered and reused with assistance of external magnetic field. After six cycles, outstanding SERS and photocatalytic capacities from FCAu-MIPs NCs could still be maintained. The FCAu-MIPs NCs with selective recognition ability to TC should thus hold tremendous applicative possibilities for highly sensitive detection and efficient degradation of TC.
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ANALYTICAL CHEMISTRY
·
2025
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IF 6.7
Yujun Cheng,Jianping Guan,Zhipeng Zhang,Ruili Li,Yao Yao,Xiaotong Sun,Qi Liu,Xiaoqing Chen
Constructing materials that possess both catalytic properties and surface plasmonic active structures presents an essential requirement in the field of in situ surface-enhanced Raman spectroscopy (SERS) monitoring. In this work, we developed a novel black raspberry-like bifunctional nanoreactor platform (HPRS@Au–Pd) with enhanced surface-enhanced Raman spectroscopy (SERS) activity (EF = 3.11 × 109) and excellent catalytic performance (e.g., |K| = 1.72 × 10–2 s–1 for 4-BTP). This platform enables sensitive and real-time monitoring of Suzuki reactions, providing an efficient and stable system for mechanistic investigations. By fitting a first-order kinetic model, we quantitatively analyzed the reaction kinetics of haloarenes on Pd NPs, revealing the direct involvement of surface-bound Pd NPs in the catalytic process. Furthermore, 4MPBA-blocking experiments using haloarenes devoid of functional groups demonstrated the absence of catalytic activity from dissolved Pd species, conclusively supporting the heterogeneous nature of the reaction. This work extends the applicability of the conclusion regarding heterogeneous catalysis to a broader range of substrates, offering valuable insights into the design and optimization of dual-functional nanoreactors. Beyond the Suzuki reactions, the versatility of this platform opens avenues for applications in other catalytic reactions, showcasing its potential in advanced catalytic studies and real-time monitoring.
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TALANTA
·
2025
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IF 6.1
Gao-Feng Wu,Jian Zhu,Guo-Jun Weng,Jian-Jun Li,Jun-Wu Zhao
To enhance the efficacy of Surface-enhanced Raman spectroscopy (SERS), noble metal nanoparticles (NMNPs) can be organized onto three-dimensional (3D) hierarchical nanostructures. This study involved constructing a 3D solid SERS substrate by layering Au–Ag core-satellite nanoparticle monolayer films (MF) on a polystyrene sphere (PS) array, termed Au–Ag core-satellite NPs-MF-PS. Optimizing the PS size was essential to maximize SERS substrate activity, with the 500 nm PS providing the best SERS enhancement factor of 1.03 × 10 7 a two-fold increase over Au–Ag core-satellite NPs MF alone. Additionally, cotinine detection was improved by using a ratio between the target molecules and internal Raman signals from the SERS substrate. Compared to conventional methods relying on the target molecule's Raman signal, this ratiometric SERS method expanded the detection range from 10 −8 - 10 −1 M to 10 −9 - 10 −1 M and reduced the detection limit from 3.67 × 10 −9 M to 1.68 × 10 −10 M. This approach represents a novel direction in creating ultra-sensitive SERS platforms with broad applications, suggesting that ratiometric SERS could further promote SERS technology advancements.
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MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Panxue Wang,Ying Liu,Xiang Li,Cen Li,Guoliang Li
Rapid and reliable determination of pathogenic bacteria is crucial for food safety and public health. Surface-enhanced Raman spectroscopy (SERS) based aptasensors have attracted increasing attention in the determination of pathogenic bacteria due to their high specificity and sensitivity. However, the sensitivity and reliability of the SERS aptasensors for the determination of pathogens were hindered by the uneven distribution of SERS tags. To tackle this limitation, a SERS aptasensor based on a hybridization chain reaction (HCR) mediated signal conversion strategy was fabricated for the determination of E. coli O157:H7. In the presence of E. coli O157:H7, the blocked aptamer specifically binds with it and releases the blocker DNA. Then the blocker DNA hybridizes with the capture DNA on the SERS probe and initiates the HCR reaction, which further leads to the formation of double stranded DNA and the reduction of the SERS signal intensity of 4-mercaptobenzonitrile (4-MBN). Under the optimal conditions, the SERS signal intensity at 1073.9 cm −1 and the logarithmic value of the concentration of E. coli O157:H7 in the range of 8 × 10 2 –8 × 10 7 CFU·mL −1 exhibited a good linear relationship, with a coefficient of determination of 0.92. The limit of detection for E. coli O157:H7 was 409 CFU·mL −1 . Besides, this aptasensor exhibited satisfactory specificity for E. coli O157:H7. In addition, it showed excellent stability in real sample analysis with relative standard deviations (RSDs) below 3.76 % (n = 3). The established SERS aptasensor employed a HCR mediated signal conversion strategy and overcame the drawback of unevenly distribution of SERS tags in classical SERS aptasensors for the determination of pathogenic bacteria. This study offered a new reference for the rapid and reliable detection of pathogens with a portable Raman spectrometer.
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BIOSENSORS & BIOELECTRONICS
·
2024
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IF 10.7
Wenze Yu,Qiujin Li,Jianing Ren,Kexin Feng,Jixian Gong,Zheng Li,Jianfei Zhang,Xiuming Liu,Zhiwei Xu,Li Yang
Herein we report a wearable sweat sensor of a Janus fabric based on surface enhanced Raman scattering (SERS) technology, mainly detecting the two important metabolites glucose and lactate. Janus fabric is composed of electrospinning PU on a piece of medical gauze (cotton), working as the unidirectional moisture transport component (R = 1305%) to collect and transfer sweat efficiently. SERS tags with different structures act as the probe to recognize and detect the glucose and lactate in high sensitivity. Core-shell structured gold nanorods with DTNB inside (AuNRs@DTNB@Au) are used to detect lactate, while gold nanorods with MPBA (AuNRs@MPBA) are used to detect glucose. Through the characteristic SERS information, two calibration functions were established for the concentration determination of glucose and lactate. The concentrations of glucose and lactate in sweat of a 23 years volunteer during three-stage interval running are tested to be 95.5, 53.2, 30.5 μM and 4.9, 13.9, 10.8 mM, indicating the glucose (energy) consumption during exercise and the rapid accumulation of lactate at the early stage accompanied by the subsequent relief. As expected, this sensing system is able to provide a novel strategy for effective acquisition and rapid detection of essential biomarkers in sweat.
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