Scientific Reports
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2020
·Vol. 10
·期 1
·1-7
·
IF 3.998
Wang Xueni,Liao Xiuling,Zhang Yimin,Wei Linyao,Pang Yuzhou
Schisandrin B (SchB) is the highest content of biphenyl cyclooctene lignans in Schisandra chinensis . It has been reported to have a variety of pharmacological effects, including anti-inflammatory, anti-oxidant, anti-cancer, heart protection, liver protection. In this study, we found that SchB can promote the proliferation of MC3T3-E1 subclone 14 cells. Meanwhile, we found that SchB can regulate the BMP2-SMADs signaling pathway by increasing gene and protein expression of those relative biomolecules. Furthermore, SchB can raise the RUNX2 and SP7 expression in both mRNA and protein levels. Since the role of BMP2-SMADs-RUNX2-SP7 signaling axis in osteoblast proliferation and differentiation has been well documented. The present experimental findings indicate that SchB could promote the proliferation and differentiation of osteoblasts through BMP2-SMADs-RUNX2-SP7 signaling axis.
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高影响因子
JOURNAL OF MATERIALS SCIENCE
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2020
·Vol. 55
·期 32
·15426-15438
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IF 3.553
Du Juan,Wu Shujing,Hu Zheyuan,Yu Ziyue,Zhao Dianbo,Bai Yanhong
Gold nanoparticles (AuNPs)-based lateral flow immunoassay (LFIA) is a widely used detection technique. Here, we developed a novel method for green synthesis of a salt-tolerant AuNPs by using aqueous extract of Damask rose petals (AEDR), for the application of Listeria monocytogenes ( L. monocytogenes ) detection by LFIA. Key parameters were optimized to achieve an improved optical LFIA performance, and the detection limit and specificity were further studied. AEDR-AuNPs were synthesized within 1 min at RT by AEDR at a concentration of 240 µg/mL, and the method was rapid, simple, economical and environmentally friendly. The AEDR-AuNPs were spherical with the size mainly ranging from 20 to 28 nm, and the salt stability was determined to be 0.4 M NaCl, which is 10 times higher than AuNPs synthesized by citrate reducing method. The optimized immunization concentration of anti- Listeria monocytogenes polyclonal antibody to AEDR-AuNPs was 60 µg/mL. The optimized antibody concentrations of test ( T ) line and control ( C ) line were determined as 1 mg/mL and 0.5 mg/mL, respectively. The detection limit was 2.5 × 10 5 CFU/mL and 2.85 × 10 5 CFU/mL in pure L. monocytogenes culture and pork tenderloin sample, respectively, and the result can be read by naked eye within 10 min. The antibody–AEDR-AuNPs LFIA strip showed no cross-reaction with all the tested bacteria including Staphylococcus aureus, Pseudomonas aeruginosa, Vibrio parahaemolyticus, Bacillus subtilis, Escherichia coli O157:H7 and Salmonella typhimurium . This approach showed a promising application for the detection of L. monocytogenes concerning food safety.
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Journal of the Australian Ceramic Society
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2020
·Vol. 57
·期 1
·81-90
·
IF 1.307
Zhao Yu,Zhou Jun,Shu Zhu,Wang Yanxin,Yakubu Yahaya,Zhang Yi,Li Xiaoqian
Previously, a way to process phosphogypsum (PG) into non-fired ceramic tile by an intermittent loading hydration process was proposed; however, its mechanical strength and especially waterproof property are unsatisfactory. Herein, PG non-fired ceramic with a porosity reduced was prepared and used as a matrix, which was then impregnated with MMA together with azobisisobutyronitrile (AIBN) and benzoyl peroxide (BPO) as initiators. After polymerization, a novel 3-3 type PMMA/PG non-fired ceramic composite with highly improved mechanical and waterproof properties was obtained. The bending strength, softening coefficient, and contact angle of the PMMA/PG composite are 21.5 MPa, 0.78, and 75.1°, which are 1.43, 1.77, and 1.68 times those of the matrix, respectively. It is found that PMMA completely covers the matrix surface and well fills the interparticle pores of dihydrate gypsum crystals inside the matrix, forming an interpenetrating and dense composite structure. This work can guide not only the utilization of waste PG but also the development of polymer/gypsum composite product. Graphical abstract We designed a facile process to prepare a novel 3-3 type PMMA/PG non-fired ceramic composite with highly improved mechanical and waterproof properties. The bending strength, softening coefficient, and contact angle of the final PMMA/PG composite are 21.5 MPa, 0.78, and 75.1°, which are 1.43, 1.77, and 1.68 times those of the matrix, respectively. The improvements originate from the delicate structure that PMMA completely covers the outside surface of the PG matrix and well fills the pores inside the PG matrix. PMMA tightly combines with dihydrate gypsum crystals and forms a relatively dense 3-3 type three-dimensional composite.
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MICROCHIMICA ACTA
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2020
·Vol. 187
·期 9
·1-10
·
IF 6.232
Guo Liying,Wu Jingyu,Xing Fei,Liu Weihua,Hao Lin,Wang Chun,Wu Qiuhua,Wang Zhi
Graphene-carbon nanosphere composite (G@CNS) was prepared via a simple hydrothermal method. The G@CNS nanocomposite was characterized by X-ray diffraction, scanning electron microscope, surface area, and porosity analysis. The G@CNS was applied as a new sorbent for solid-phase extraction of five carbamate pesticides (tsumacide, carbaryl, isoprocarb, bassa, diethofencarb) prior to quantitative determination by high-performance liquid chromatography with ultraviolet detection at 208 nm. Some experimental parameters including desorption conditions, sample pH, sample volume, and loading rate were studied carefully. Under the optimized condition, the method provided good linearity ranging from 0.3 to 100.0 ng mL −1 with low limits of detection of 0.10–0.20 ng mL −1 for grape juice, 0.10–0.30 ng mL −1 for blend fruit juice, and 0.10–0.20 ng mL −1 for water sample. Good method recoveries (80.2–110%) with relative standard deviations less than 7.2% and high enrichment factors (167–293) were achieved. Results demonstrated that this novel G@CNS can serve as a promising alternative sorbent for more applications.
In this work, a graphene-carbon nanosphere (G@CNS) composite was synthesized via a simple hydrothermal method. Then, the G@CNS was served as a novel sorbent for solid-phase extraction of five carbamate pesticides (tsumacide, carbaryl, isoprocarb, bassa, diethofencarb) in juice and environmental water samples, followed by their quantitative analysis with high-performance liquid chromatography-ultraviolet detection.
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JOURNAL OF MATERIALS SCIENCE
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2020
·Vol. 55
·期 28
·13980-13994
·
IF 3.553
Li Wenjun,Yang Yue,Ma Chaoyun,Song Yiju,Hong Chenglin,Qiao Xiuwen
A novel sandwich-type electrochemical immunosensor based on cuprous oxide (Cu 2 O)@copper-metal organic framework (Cu-MOF)@gold nanoparticles (Au NPs) core–shell structure was proposed. Cu 2 O@Cu-MOF@Au attached with horseradish peroxidase (HRP) triple-catalyzed hydrogen peroxide (H 2 O 2 ) for detecting CEA antigen. In this work, 50 nm Cu 2 O nanospheres were prepared. Using Cu 2 O nanospheres as the core, Cu-MOF was coated on the surface of Cu 2 O nanospheres by reaction of Cu 2 O and ligands. Subsequently, chloroauric acid was reduced to Au NPs without adding any reducing agent and Au NPs dispersed on the surface of Cu-MOF. Finally, the Cu 2 O@Cu-MOF@Au-HRP-Ab 2 nanoprobe was successfully prepared. Immunosensors had good performance due to the following reasons. Firstly, Cu 2 O, Cu-MOF and HRP have excellent catalytic properties for hydrogen peroxide. In addition, the synergistic effect of Cu 2 O, Cu-MOF and HRP promoted the reduction of hydrogen peroxide and tripled amplification of electrical signals. Here, the successfully prepared immunosensor detected carcinoembryonic antigen (CEA) sensitively, the detection range was 50 fg/mL–80 ng/mL, and the detection limit was 17 fg/mL. Besides, the proposed immunosensor had good stability, selectivity and specificity. Detection in human serum samples had good results without matrix effect. This provided a new idea for the clinical detection of tumor markers: "metal oxide—metal organic framework—precious metal nanoparticles" (MMP). Graphic abstract
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BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
·
2020
·Vol. 25
·期 4
·589-598
·
IF 2.213
He Jinjia,Chen Yi,Dai Luyao,Yao Jiachao,Mei Yu,Hrynsphan Dzmitry,Tatsiana Savitskaya,Chen Jun
Acrylic acid is used in various industrial applications but inflicts harm to human health and causes environmental pollution. A new bacterium, identified as Rhodococcus ruber JJ-3, was isolated, which can degrade high concentrations of acrylic acid rapidly and completely. Experimental results showed that the strain can achieve complete degradation of 1000 mg·L −1 acrylic acid in 11 h under the following conditions: pH 7, temperature 35°C, and inoculation quantity 15%. A high concentration of acrylic acid (2000 mg·L −1 ) can be completely removed in 28 h. According to the Monod model, the maximum specific degradation rate ( v max ) and half saturation rate constant ( K S ) of the strain were 0.85 h −1 and 101.83 mg·L −1 , respectively. The results of carbon balance revealed that 54.6% carbon was assimilated by R. ruber JJ-3 as biomass, and 43.0% carbon was mineralized into CO 2 . Furthermore, glycerol and lactic acid were measured as intermediates, and the possible degradation pathway was proposed during the biodegradation of acrylic acid. These results suggested that R. ruber JJ-3 completely degrades acrylic acid and might have a potential environmental implication in the purification of acrylic acid-contaminated environments.
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BMC MICROBIOLOGY
·
2020
·Vol. 20
·期 1
·1-12
·
IF 2.989
Wang Juanjuan,Li Ruochen,Zhang Hui,Wei Gehong,Li Zhefei
Background Excessive application of chemical fertilizer has exerted a great threat to soil quality and the environment. The inoculation of plants with plant-growth-promoting rhizobacteria (PGPR) has emerged as a great prospect for ecosystem recovery. The aim of this work to isolate PGPRs and highlights the effect of bacterial inoculants on available N/P/K content in soil and on the growth of wheat under conditions of reduced fertilizer application. Results Thirty-nine PGPRs were isolated and tested for their growth-promoting potential. Thirteen isolates had nitrogen fixation ability, of which N9 ( Azotobacter chroococcum ) had the highest acetylene reduction activity of 156.26 nmol/gh. Eleven isolates had efficient phosphate solubilizing ability, of which P5 ( Klebsiella variicola ) released the most available phosphorus in liquid medium (231.68 mg/L). Fifteen isolates had efficient potassium solubilizing ability, of which K13 ( Rhizobium larrymoorei ) released the most available potassium in liquid medium (224.66 mg/L). In culture medium supplemented with tryptophan, P9 ( Klebsiella pneumoniae ) produced the greatest amount of IAA. Inoculation with the bacterial combination K14 + 176 + P9 + N8 + P5 increased the alkali-hydrolysed nitrogen, available phosphorus and available potassium in the soil by 49.46, 99.51 and 19.38%, respectively, and enhanced the N, P, and K content of wheat by 97.7, 96.4 and 42.1%, respectively. Moreover, reducing fertilizer application by 25% did not decrease the available nitrogen, phosphorus, and potassium in the soil and N/P/K content, plant height, and dry weight of wheat. Conclusions The bacterial combination K14 + 176 + P9 + N8 + P5 is superior candidates for biofertilizers that may reduce chemical fertilizer application without influencing the normal growth of wheat.
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CELLULOSE
·
2020
·Vol. 27
·期 7
·4007-4018
·
IF 4.21
Zhang Tao,Zhao Yan,Silverstein Michael S.
Abstract Cellulose is one of the most abundant resources in nature. Cellulose-based porous monoliths have been fabricated by direct drying of oil-in-water high internal phase emulsions (HIPEs) stabilized using either cellulose derivatives or surface-modified cellulose. The resulting cellulose-based porous polymers, however, were usually fragile, reflecting the lack of crosslinking. Herein, we report a new strategy to fabricate cellulose-based porous materials (polyurethane polyHIPEs, PU polyHIPEs) templated within non-aqueous HIPEs through the covalent crosslinking between isocyanates and unmodified cellulose. The PU polyHIPEs exhibited interconnected macroporous structures and exhibited tunable wettability from hydrophilicity/oleophilicity to hydrophobicity/oleophilicity. The PU polyHIPEs were able to absorb a wide variety of oils, exhibiting relatively large capacities and high absorption rates. We further showed that the PU polyHIPEs were robust, and they did not fail under compressive stress even at strains of 70%. The robustness, large absorption capacities, rapid absorption, and hydrophobicity/oleophilicity make these cellulose-based PU polyHIPEs suitable for oil absorption and/or oil–water separation applications. Graphic abstract
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Scientific Reports
·
2020
·Vol. 10
·期 1
·1-10
·
IF 3.998
Chen Junjun,Li Hailong,Zhang Lihui,Du Chao,Fang Tao,Hu Jian
With the rapid development of wearable and portable electronic devices, it is increasingly important to develop conductive paper-like films (CPFs) with the characteristics of light, thin and self-supporting. In this paper, nanofibrillated cellulose (NFC) was used as reinforcing phase of film-forming to combine with graphene oxide (GO). Then graphene-based CPFs were prepared by directly reducing the GO/NFC composite film without any additional adhesives, which effectively avoided the difficulties of dispersion and combination with other materials caused by direct using of high content graphene. Meanwhile, three representative reduction methods for direct reduction of GO/NFC composite films were also compared. The results show that 450 °C thermal reduction and hydroiodic acid reduction were more effective than ascorbic acid reduction. On this basis, hydroiodic acid reduction and thermal reduction were used to discuss the effect of NFC addition to the conductivity of the film. This occured when increasing the content of NFC from 10% to 50%, the electrical conductivity of the composite film by hydroiodic acid reduction decreased from 153.8 S/m to 22.2 S/m. While the conductivity of composite film increased first and then decreased after thermal reduction both at 450 °C and 550 °C. What’s more, when NFC content was about 16.6% the electrical conductivity reached a high level which was 86.21 S/m and 168.9 S/m, respectively. This study provides a groundwork for the further development of graphene-based CPFs with low square resistance and high conductivity in large-scale preparation.
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Scientific Reports
·
2020
·Vol. 10
·期 1
·1-15
·
IF 3.998
Zhou Qiying,Chen Wenhua,Jiang Xia,Liu Hongying,Ma Shenggui,Wang Bangda
A novel nitrogen-containing graphitic mesoporous carbon was prepared through MnO-templated method using polyacrylonitrile (PAN) as both carbon and nitrogen sources, and MnCO 3 as both template and catalyst precursors. The effects of preparation conditions on the physicochemical properties of obtained samples were systematically investigated. The results showed that as the decrease of the weight ratios of PAN and MnO (2:1–1:4), the increase of carbonization temperature (700–900 °C) and pre-oxidation temperature (180–200 °C), the samples had higher specific surface area, mesopores volume and ratios, up to 507 m 2 /g, 0.824 cm 3 /g and 96.83%, respectively. Moreover, the prepared samples presented relatively high graphitic degree and nitrogen contents (~2.21%). The adsorption capacity for acid red 88 (AR88) was as high as 309 mg/g, which were dramatically affected by the mesoporous properties and C- and N-containing groups on the surface of prepared carbon. The rich graphic carbon and pyridine-N in mesoporous carbon generated π-π dispersion and electrostatic interaction with AR88, respectively, which jointly were responsible for the adsorption process. The results of the isotherm and kinetic studies indicated that the AR88 adsorption on mesoporous carbon could be well depicted using Langmuir model and pseudo-2 nd -order model.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2020
·Vol. 27
·期 11
·11990-12000
·
IF 3.056
Cui Yanping,Thathsarani Nimesha,Peng Liang,Gao Ya,Lei Lidan,Zhou Zhengwei,Liang Lili,Shi Xiaoyan
Innovative paramagnetic one-dimensional (1-D) core-shell Fe-Ag@AgCl visible light-driven photocatalysts are synthesized through a template-assisted electrodeposition method trailed by FeCl 3 in-situ oxidation. The metallic nature of Fe-Ag@AgCl is confirmed through scanning electron microscopy (SEM) and crystal nature through X-ray diffraction (XRD). The controllable diameter of Fe-Ag is obtained through the selection of hollow size of the polycarbonate (PC) template. Electron impedance spectroscopy (EIS) confirms through the introduction of Fe to the Ag core that has prolonged the recombination of electron and hole. Escherichia coli ( E. coli ) are employed as the target bacteria to evaluate the photocatalytic disinfection performances. A total of 1.30 mg of Fe-Ag@AgCl is proved to be able to completely inactivate 10 7 CFU (colony forming units)/mL after 120 min of visible light irradiation. The transition electron microscopy (TEM) confirms the stability of the material after the photo reaction. As Fe-Ag@AgCl possesses magnetic properties, the material is recovered through the application of an external magnetic field. SEM images and results of 3D emission extraction matrix (EEM) depict that the bacteria cell death is caused by membrane permeability changes caused by the reduction of membrane associated proteins.
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CELLULOSE
·
2020
·Vol. 27
·期 6
·3455-3468
·
IF 4.21
Xue Chao-Hua,Wu Yue,Guo Xiao-Jing,Liu Bing-Ying,Wang Hui-Di,Jia Shun-Tian
Functional textiles are ideal substrates for wearable electronics. Herein, superhydrophobic, flame-retardant and conductive cotton fabrics were fabricated by sequential assembly of poly(ethylenimine), ammonium polyphosphate and carbon nanotubes, followed by post-treatment with poly(dimethylsiloxane). The resulting fabrics possessed excellent superhydrophobic stability toward acid, alkali, organic solvent, UV irradiation, abrasion and long-time laundering. Meanwhile, when suffering to fire, the coated fabric could generate an efficient char layer and extinguish the fire to protect the cotton fiber from forming flame. Furthermore, this conductive cotton fabric exhibited stable sensing ability in contact with water droplets, showing wide potential application in wearable electronics as multifunctional smart textiles.
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危险品化学品经营许可证（带存储）