ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2025
·
IF 0
Al-Ghamdi Azza A.,Ibrahim Sherif M.,Lu Xingmei,Xin Jiayu,El-Sayed Ibrahim El-Tantawy,Galhoum Ahmed A.,Wageh Swelm.
A sustainable biosorbent, silver nanoparticles-decorated coffee-ground waste (CWAg), was synthesized through a simple in-situ reduction method. CWAg is extensively characterized via SEM–EDX, PZC, FTIR, XRD, HR-TEM, and XPS analyses. The biosorbent was tested to remove chromium (Cr(VI)) and methylene blue (MB) from wastewater, and its antibacterial properties was evaluated. CWAg demonstrated efficient sorption efficiencies, achieving ⁓149.9 mg/g at 323 K and pH: 2.0 for Cr(VI) and ⁓113.4 mg/g at 293 K and pH: 6.0 for MB, at a biosorbent dose of 1 g/L. The equilibration times were 90 and 120 min for Cr(VI) and MB, with half-sorption times (t HST ) of 9.2 and 13.9 min, respectively. The sorption mechanisms were successfully fitted with Sips isotherm and Pseudo-second-order models. Simultaneous sorption of MB and Cr(VI) at pH 2.0 exhibited antagonism, while a synergistic effect was observed at pH 6.0. Desorption and regeneration were achieved using NaOH and HCl solutions for Cr(VI) and MB, respectively. The efficiencies were just reduced by 9–13%. Finally, CWAg exhibited remarkable effectiveness for removing Cr(VI) from complex tannery effluent. Moreover, CWAg demonstrated positive antibacterial effects against both Gram-positive and Gram-negative bacteria, surpassing raw coffee-ground waste, underscoring the significant role of silver nanoparticles in enhancing bacterial inhibition.
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MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 8
·1-9
·
IF 5.3
Zhang Qijia,Wang Yuting,Ma Dandan,Pan Xing,Sheng Enze,Shen Jiachen,Liang Dongbing,Wang Chao,Qian Chen,Qian Wenhui,Zhu Dong
A highly sensitive dual-recognition fluorescence amplification method is presented for lipopolysaccharide (LPS) detection based on boronic functionalized aptamer macroarrays with dual-recognition and isothermal amplification. The surface of the polystyrene microplate was firstly carboxylated, and then, 3-aminophenylboronic acid was conjugated to the carboxyl groups through EDC/NHS reaction, creating boronic acid groups as the capture moiety for LPS. A recognition DNA aptamer labeled with the fluorescent dye 6-FAM, which exhibits specificity towards LPS, was selected as the signal reporting moiety. By introducing primers and Klenow enzyme, the fluorescent-labeled aptamers are released from the microplate bottom, and double-stranded structures were formed via isothermal amplification. The addition of SYBR Green I, which strongly fluoresces upon binding to the double-stranded structures, enables signal amplification and detection. This detection method exhibits a linear range of 1–10,000 ng/mL and has a detection limit as low as 401.93 pg/mL. This analytical approach shows high selectivity and sensitivity and may serve as a universal platform in lipopolysaccharide detection.
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高影响因子
CHROMATOGRAPHIA
·
2025
·
IF 1.3
Jiang Qiaoling,Wu Qiaoli,Chen Shuhang,Zeng Tianfu,Tong Xiaoyi
This study introduces a rapid LC–MS/MS method designed to simultaneously quantify 14 water-soluble vitamins and their metabolites in human serum. The protein precipitation with metaphosphoric acid and stable isotope dilution techniques are utilized in this method. An Agilent XDB C18 column and dynamic ionization mode switching are adopted, enabling the completion of a single sample analysis within 5 min. The method has undergone rigorous validation. The lower limits of quantification (LLOQs) range from 0.3 to 400 ng/mL, with recovery rates between 80 and 120%. Intra-day and inter-day relative standard deviations (RSDs) are below 15%. The matrix effects are controlled within ± 15%, and the analyte carryover is less than 20% of the LLOQ. The stability tests have demonstrated that samples remain stable at room temperature (25 °C) for 10 h, at − 20 °C for 7 days, and can withstand one freeze–thaw cycle. Extracted samples are stable in the autosampler (4 °C) for 24 h. The method was applied to serum samples from 150 healthy individuals. Non-parametric statistical analysis was carried out to establish clinical reference intervals, which provides valuable data for the clinical evaluation of vitamin and metabolite levels in serum.
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Chinese Medicine
·
2025
·
IF 5.7
Ruiyu Wu,Runping Liu,Ranyun Chen,Yijie Li,Xiaoyong Xue,Yinhao Zhang,Fanghong Li,Jiaorong Qu,Lingling Qin,Chen Wang,Xiaojiaoyang Li
Background Obesity is frequently linked to chronic systamic inflammation and presents significant challenges to public health. Aurantio-obtusin (AO) boosted the brown adipose tissue (BAT) thermogenesis in diet-induced obesity. However, the specific mechanisms by which injured mitochondria-related damage signals derived from overwhelmed BAT can transmit to liver and exacerbate metabolic disorders and whether AO can reverse this process remain unknown. Materials and methods After applying high-fat diet and glucose-fructose water (HFHS)-induced obesity mice, different BAT transplant procedures and primary BAT adipocytes, we investigated the anti-obesity effects and mechanism of AO through RNA sequencing and biology techniques. Results AO improved whole-body lipid accumulation, mitochondrial metabolism in BAT and hepatic inflammation in HFHS-induced obesity mice. Interscapular transplant of BAT-derived from obese donor mice triggered hepatic inflammation of chow diet-fed recipient mice, which was protected by AO. Furthermore, the transplantation of BAT-derived from AO-treated mice protected hepatic inflammation in obese mice. In vivo and in lipid-challenged primary BAT adipocytes, AO decreased kexin type 9 (PCSK9), prevented mPTP opening and mitochondrial DNA (mtDNA) release in extracellular vesicles (EVs) manner by inhibiting the acetylation of cyclophilin D associated with adenine nucleotide translocase, suppressing oligomerization of voltage-dependent anion channel 1 and activating mitophagy. Ultimately, AO inhibited mtDNA-containing EVs-induced cyclic GMP-AMP synthase/stimulator of interferon genes (STING) activation and hepatic inflammation, which was confirmed by Sting −/− mice. Conclusion AO not only improves thermogenesis and mitochondrial function of BAT but also prevents liver inflammation by repairing mitochondrial function and blocking the transfer of mtDNA from BAT to the liver. Graphic abstract Supplementary Information The online version contains supplementary material available at 10.1186/s13020-025-01097-y.
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高影响因子
RARE METALS
·
2025
·
IF 11
Wang Ding,Qin Rui-Jie,Yu Jie-Jie,Hu Jin-Wu,Zhang Wen-Hui,Xu Hui,Xu Jing-Cheng,Liao Qiao-Bo,Li Hui-Jun,Wang Xu-Hui
The semiconductor gas sensors used for xylene gas detection in real time has been restricted by the inadequate sensitivity and selectivity. Constructing a bilayer cascade sensor with the catalysis-gas sensitivity synergistic is considered as an effective solution. Herein, the Ag@CeO 2 nanosheets are synthesized by heat treating the Ag@Ce-MOF, which synthesized via solvothermal method. The morphological evolution of cerium metal–organic framework (Ce-MOF), regulated by Ag ions, is investigated, and the transformation mechanism is proposed. The bilayer sensors were constructed by using WO 3 nanofibers, prepared via the electrospinning method, as the sensitive layer and the Ag@CeO 2 nanosheets as the catalytic layer, respectively. The bilayer sensors exhibit remarkable performance in response to xylene. The response value ( R a / R g ) of WO 3 /Ag@CeO 2 sensor to 10 ppm xylene gas reaches 32.13 at the operating temperature of 160 °C. Additionally, the sensor displays an exceptional response to even trace amounts of xylene, as low as parts per billion (ppb). The catalysis-gas sensitivity synergistic mechanism was elucidated by capturing catalytic intermediates using online mass spectrometry. These findings provide a novel strategy for benzene series (BTEX) sensor and offer a novel approach to prepare two-dimensional Ce-MOF and its derived materials with tailored properties. Graphical abstract
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MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 2
·1-11
·
IF 5.3
Zhang Xiaoyu,Sun Ruimeng,Zheng Haoran,Qi Yanfei
A spherical nucleic acid (SNA, AuNPs-aptamer) into CRISPR/Cas12a system combined with poly T-template copper nanoparticles as fluorescence reporter was fabricated to establish an amplification-free sensitive method for Staphylococcus aureus ( S. aureus ) detection. This method, named PTCas12a, utilizes the concept that the bifunction of SNA recognizes the S. aureus and triggers the Cas12a cleavage activity. Then, the Cas12a enzyme cleaves the Poly T40 to generate a signal change in Poly T-Cu fluorescence, indicating the presence or absence of the target bacteria. The PTCas12a platform demonstrated a detection limit as low as 3.0 CFU/mL (3 N/S) in a wide response range of 1.0 × 10 1 –1.0 × 10 6 CFU/mL for S. aureus detection, which holds significant potential in ensuring food safety and preventing the spread of diseases. Graphical abstract
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高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 9
·1-14
·
IF 5.3
Deng Tingliu,Wu Wuming,Zhou Jingjing,Zeng Qin,Wang Heye,Deng Chunyan
A novel bacteriophage-targeted electrochemical biosensor designed for accurate and quantitative detection of live Salmonella in food samples is presented. The biosensor is simply constructed by electrostatic immobilizing bacteriophages on MXene-nanostructured electrodes. MXene, renowned for its high surface area, biocompatibility, and conductivity, serves as an ideal platform for bacteriophage immobilization. This allows for a high-density immobilization of bacteriophage particles, achieving approximately 71 pcs μm −2 . Remarkably, the bacteriophages immobilized MXene nanostructured electrodes still maintain their viability and functionality, ensuring their effectiveness in pathogen detection. Therefore, the proposed biosensor exhibited enhanced sensitivity with a low limit of detection (LOD) of 5 CFU mL −1 . Notably, the biosensor shows excellent specificity in the presence of other bacteria that commonly contaminate food and can distinguish live Salmonella from a mixed population. Furthermore, it is applicable in detecting live Salmonella in food samples, which highlights its potential in food safety monitoring. This biosensor offers simplicity, convenience, and suitability for resource-limited environments, making it a promising tool for on-site monitoring of foodborne pathogenic bacteria.
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高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 11
·1-11
·
IF 5.3
Shao Huikai,Zhao Lingguo,Liu Zhenping,Li Jinqi
A simple method for highly selective and sensitive prostate-specific antigen (PSA) detection using a molecularly imprinted electrochemical sensor is presented. The sensor was developed through an epitope imprinted strategy combined with electrochemical measurement techniques. An epitope molecularly imprinted polymer (EMIP) film was constructed on a AuNPs-coated gold electrode surface through electropolymerization, utilizing the C-terminus epitope of PSA (KWIKDTIVANP) as the template molecular and o -phenylenediamine as the functional monomer. The characteristics of EMIP film were investigated by using a scanning electron microscope and electrochemical test methods, including electrochemical impedance spectroscopy and cyclic voltammetry. Key parameters such as electropolymerization cycles, elution and rebinding times, and the molar ratio of template molecular to functional monomer were systematically optimized. The sensor demonstrated a detection limit (LOD) of 0.31 fg/mL and exhibited an excellent linear response towards PSA concentration ranging from 1.0 fg/mL to 0.1 µg/mL. Furthermore, the EMIP sensor showed excellent selectivity against other biological macromolecules, such as bovine serum albumin, human serum albumin, alpha-fetoprotein, and carcinoembryonic antigen. With recoveries between 95.89 and 106.04% for PSA detection in human serums the EMIP/AuNPs/AuE electrochemical sensor showed great potential in real sample analysis. Graphical Abstract
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高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2012
·Vol. 48
·期 2
·805-811
·
IF 2.015
Wang Shi-Jie,Hu Jiang-Bo,Wang Yin-Yin,Luo Fang
Rare-earth (RE) complexes can be easily adsorbed on the surface of graphene oxide sheets (GOSs) by a simple noncovalent approach. The resulting nanocomposite materials, RE complexes functionalized GOSs, have been comprehensively characterized via transmission electron microscopy, X-ray photoelectron spectroscopy, FT-IR spectra, X-ray diffraction, and thermogravimetric analysis. The luminescence properties of GOSs-RE were also investigated. Under the excitation of UV light, these samples display the characteristic emissions of Eu 3+ ions. The photophysical investigations revealed that the presence of GOSs do not quench the lanthanide-centered luminescence stemming from the characteristic electronic transitions within the 4 f shell of the Eu 3+ ions. The GOSs–RE complex hybrids and their dispersion can emit bright red luminescence. Moreover, the presence of GOSs can elongate the fluorescence lifetime of the RE complexes to 983 μs. These properties make them useful in many practical fields, such as in the lighting and biological labeling.
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高影响因子
JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
·
2012
·Vol. 295
·期 1
·167-177
·
IF 1.52
He F. F.,Wang H. Q.,Wang Y. Y.,F Wang X.,Zhang H. S.,Li H. L.,Tang J. H.
A new complex of N , N ′-bis(3-allyl salicylidene) o -phenylenediamine and thorium(IV) (Th(IV)) was synthesized and used as the functional monomer for a novel Th(IV) magnetic ion-imprinted polymer; this polymer was synthesized using a surface imprinting technique that included the modified magnetic Fe 3 O 4 particle and used tetraethyl orthosilicate, 3-Aminopropyltriethoxysilane and maleic anhydride in the process. The magnetic polymer was characterized using FT-IR, and powder- and single crystal-XRD. The behavior of Th(IV) was investigated using batch experiments. At pH 4.5, the uptake capacity of this adsorbent and that of the non-imprinted polymer was 42.54 and 14.10 mg g −1 , respectively, and the relative selectivity coefficient values of the synthesized adsorbent for Th(IV)/La(III), Th(IV)/Ce(III), Th(IV)/Nd(III), and Th(IV)/U(VI) were 82.2, 93.1, 21.0 and 62.4 times greater than that of the non-imprinted matrix, respectively. In addition, the Th(IV) adsorption process using Fe 3 O 4 @SiO 2 -IIP follows pseudo-second-order reaction kinetics and the Langmuir adsorption isotherm. The thermodynamic parameters also suggest that the adsorption of Th(IV) onto Fe 3 O 4 @SiO 2 -IIPs was a spontaneous and endothermic process.
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高影响因子
RESEARCH ON CHEMICAL INTERMEDIATES
·
2012
·Vol. 38
·期 9
·2237-2243
·
IF 0.697
Zhou Minghao,Xiao Guomin,Hong Mei
(6R)- and (6S)-5,6,7,8-tetrahydrofolic acid were resolved by reversed-phase HPLC on a C-18 column with hydroxypropyl-β-cyclodextrin (HP-β-CD) in the mobile phase. As the concentration of HP-β-CD increased from 0 to 45 mM, resolution increased and retention times decreased dramatically. The influence of different mobile phase pH, column temperature, and some other factors were evaluated, and we obtained the optimal HPLC separation conditions.
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高影响因子
Food and Bioprocess Technology
·
2024
·
IF 5.3
Gao Weilan,Zheng Zhan,Chen Rongyu,Zhang Na,Wang Xuehua,Zhu Zhenzhou,Li Shuyi,Cong Xin
Selenylation modification is an effective way to improve the nutritional and health effects of tea polysaccharide, but the effects of different selenylation methods on the structure and efficacy of polysaccharides remain unclear. In this study, a series of chemically selenized tea polysaccharides (CSe-TPS, selenium content of 129.98 ~ 1454.99 µg/g) were prepared by Na 2 SeO 3 -HNO 3 method, which were promoted by heating (HCSe-TPS), ultrasound (UCSe-TPS), and pulse electric field (PCSe-TPS) treatments, respectively. These different CSe-TPSs were systematically compared with ordinary tea polysaccharides (Ord-TPS) and natural selenium-enriched tea polysaccharides (NSe-TPS) in aspect of molecular structure and biological functions. The results of molecular structure and apparent morphology showed that the particle size ( p < 0.05) and the thermal stability of Ord-TPS were reduced after selenylation modification, while the absolute potential of polysaccharide was increased ( p < 0.05), coupled with altered monosaccharide composition and changed apparent morphology. However, the main functional groups and the secondary structure of Ord-TPS did not change. The antioxidant activities of tea polysaccharides after selenylation were improved as well as their inhibitory effects on carbohydrate digestive enzymes ( p < 0.05). The total antioxidant capacity of PCSe-TPS (selenium content of 240.66 ± 1.43µmol/g) was enhanced by 85.41% compared with Ord-TPS (selenium content of 129.80 ± 3.42 µmol/g), while its IC 50 values on inhibition of α-amylase (2.91 ± 0.18 mg/mL) and α-glucosidase (0.18 ± 0.01 mg/mL) were significantly lower than other tea polysaccharides ( p < 0.05), which suggested best antioxidant and hypoglycemic activity. Moreover, tea polysaccharides with higher selenium content and smaller particle size showed better antioxidant and hypoglycemic activities. This research will provide new strategies for the application of tea polysaccharides.
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高影响因子
危险品化学品经营许可证（带存储）