ENERGY & FUELS
·
2024
·
IF 5.2
Li Zhao,Hao Fu,Yuan-gu Xia,Ji Liu,Bin Hu,Zhan-jun Cheng,Xi Luo,Dan Yan,Ji-hong Li,Qiang Lu
Pyrolysis is a promising thermochemical conversion technology, which can convert biomass and cellulose into value-added products. Herein, a new approach of oxalic acid-assisted staged fast pyrolysis (OASFP) of cellulose was developed, achieving the coproduction of 1,4:3,6-dianhydro-α-d-glucopyranose (DGP), furfural (FF), and formic acid (FA) in separated pyrolysis stages. The lab-scale tests demonstrated that a selectivity of 71.6% and a yield of 31.5 wt % for FA in the aqueous phase, as well as a selectivity of 55.6% and a yield of 1.2 wt % for FF in the organic phase, were achieved in the primary pyrolysis stage under the optimized conditions (primary pyrolysis temperature of 220 °C and cellulose-to-oxalic acid (OA) ratio of 1:3). During the subsequent secondary pyrolysis process at 400 °C, the decomposition of the solid residue achieved a yield of 7.2 wt % and a selectivity of 32.7% for DGP. The interaction between OA and cellulose during the primary pyrolysis stage was essential for DGP generation. The formation mechanism of major products and the role of OA were revealed based on experiments and density functional theory calculations. In total, the OASFP of cellulose offers new possibilities and opportunities for biomass utilization.
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Food Bioscience
·
2025
·
IF 5.9
Shu He,Zhengli Lu,Rurui Li,Chun Chen,Xiong Fu,Kin Weng Kong,Amin Ismail,Xuechun Zhang,Zhenxing Wang
This study evaluated the chemical composition and bioactivity of nine Dendrobium officinale from three cultivation modes (greenhouse, GC; understory, UC; and simulated wild, SWC) across China. Results demonstrated that UC-cultivated samples exhibited significantly higher flavonoid levels, antioxidant capacity, and α-glucosidase inhibition activity than those under GC/SWC modes. Using GC-IMS and UHPLC-TOF-MS, metabolomic profiling identified 27 volatile and 15 non-volatile biomarkers between different cultivation modes. Further KEGG analysis revealed the key signaling pathways of differential metabolites under different cultivation modes, including aminobenzoate degradation, phenylpropanoid biosynthesis, and phenylalanine metabolism. Overall, the cultivation mode significantly influences the chemical composition and biological activity of Dendrobium officinale , with the UC mode being relatively preferable. However, factors such as geographical distribution also play an important role. These findings provide actionable insights for quality control, cultivation optimization, and functional food/pharmaceutical applications of Dendrobium officinale .
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Journal of Functional Foods
·
2020
·
IF 3.701
Liu Tang,Xiaolei Li,Liping Wan,Huiling Wang,Qianting Mai,Zixin Deng,Hong Ding
The present research was designed to evaluate the protective effects and underlying mechanisms of different ratios of linoleic acid (LA) and α-linolenic acid (ALA), two essential polyunsaturated fatty acids (PUFAs) fatty acids, on atopic dermatitis (AD)-like skin lesions induced by 2, 4-dinitrofluorobenzene (DNFB). The dorsal flank and ear pinnas of mice were sensitized and challenged with DNFB for four weeks in order to establish AD model. Simultaneously, the mice were orally administered different ratios of LA/ALA (v/v; 4:1 or 1:1), or dexamethasone (Dex; 0.1 mg/kg). Results showed that different LA/ALA ratios, especially a ratio of 4:1 treatment, could effectively ameliorate DNFB-induced skin lesions, ear edema, scratching behavior and skin inflammation, as reflected by the reduced inflammatory blood cells, IgE serum levels, and skin levels of the pro-inflammatory lipid mediators, including PGE 2 and LTB 4 . These findings were further confirmed by skin pathological examination. Moreover, LA/ALA administration markedly suppressed the proliferation and infiltration of T cells, the production of T helper cell type 1 (Th1), Th2 and Th17 cytokines in dorsal skin and serum of DNFB-treated mice. Western blotting and immunohistochemistry staining revealed that LA/ALA downregulated the protein expressions of JAK2 and STAT4, and thus reducing the synthesis of type Th1 cytokine IFN-γ. Taken together, these results suggest that optimal nutrition ratio of LA/ALA has beneficial effects on AD-like symptoms, which provide theoretical basis for its therapeutic potential in the clinical prevention and treatment of AD.
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BIOMEDICINE & PHARMACOTHERAPY
·
2020
·
IF 4.545
Zhishen Xie,Gai Gao,Hui Wang,Erwen Li,Yong Yuan,Jiangyan Xu,Zhenqiang Zhang,Pan Wang,Yu Fu,Huahui Zeng,Junying Song,Christian Hölscher,Hui Chen
Dual-PPAR-α/γ agonist has the dual potentials to improve insulin resistance (IR) and hepatic steatosis associated with obesity. This study aimed to investigate whether dehydroabietic acid (DA), a naturally occurred compound, can bind to and activate both PPAR-γ and PPAR-α to ameliorate IR and hepatic steatosis in high-fat diet (HFD)-fed mice.. We found that DA formed stable hydrogen bonds with the ligand-binding domains of PPAR-γ and PPAR-α. DA treatment also promoted 3T3-L1 differentiation via PPAR-γ activation, and mitochondrial oxygen consumption in HL7702 cells via PPAR-α activation. In HFD-fed mice, DA treatment alleviated glucose intolerance and IR, and reduced hepatic steatosis, liver injury markers (ALT, AST), and lipid accumulation, and promoted mRNA expression of PPAR-γ and PPAR-α signaling elements involved in IR and lipid metabolism in vivo and in vitro , and inhibited mRNA expression of pro-inflammatory factors. Therefore, DA is a dual-PPAR-α/γ and PPAR-γ partial agonist, which can attenuate IR and hepatic steatosis induced by HFD-consumption in mice.
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PubMed
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Polymers
·
2022
·Vol. 14
·期 14
·2872
·
IF 4.967
Zhengshi Li,Huimin Lin,Shanwei Shi,Kai Su,Guangsen Zheng,Siyong Gao,Xuan Zeng,Honglong Ning,Meng Yu,Xiang Li,Guiqing Liao
Bone healing is a complex process that requires the participation of cells and bioactive factors. Stromal derived factor-1 α (SDF-1α) and magnesium ions (Mg2+) both are significant bioactive factors for cell recruitment and osteogenesis during bone regeneration. Thus, a bifunctional hydrogel containing a sequential delivery system is fabricated to improve osteogenesis. During sequential delivery of the hydrogel, SDF-1α is predominantly released at the early stage of bone mesenchymal stem cells (BMSCs) recruitment, while Mg2+are constantly delivered at a later stage to improve osteogenic differentiation of recruited cells. In addition, due to the early release of SDF-1α, the hydrogel showed strong BMSCs recruitment and proliferation activity. Mg2+can not only induce up-regulation of osteogenic gene expression in vitro, but also promote bone tissue and angiogenesis in vivo. Taken together, the injection of xanthan gum-polydopamine crosslinked hydrogel co-loading SDF-1α and Mg2+(XPMS hydrogel) provides a novel strategy to repair bone defects.Keywords:hydrogel;BMSCs recruitment;sequential drug release;osteogenic differentiation;bone regeneration
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JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2021
·Vol. 101
·期 10
·4173-4182
·
IF 3.639
Bin Jiang,Linlin Wang,Meichan Wang,Shuang Wu,Xiaojing Wang,Dongmei Li,Chunhong Liu,Zhibiao Feng,Yujie Chi
BACKGROUND α -lactalbumin ( α -La) is of great interest to the industry as a result of its excellent functional properties and nutritional value. Aqueous two-phase flotation (ATPF) of thermo-sensitive polymer poly (ethylene glycol-ran-propylene glycol) monobutyl ether (UCON) and KH 2 PO 4 was applied to directly separate and purify α -La from milk whey, which was purposed to simplify the production process and reduced cost of production. RESULTS The effect of ATPF composition and operating parameters on the flotation efficiency ( E ) and purity of α -La were investigated. The optimal conditions included 2 min of premixing time, 30 mL min −1 flow velocity and 20 min of flotation time, whereas the composition conditions comprised 35.0 mL 0.18 g mL −1 phosphate solution (containing 10% (cow milk whey/salt solution, v/v) cow milk whey, 50 ppm defoamer and 2 g NaCl) and 5.0 mL of 40% ( w/w ) UCON solution. Under the optimal conditions, E of α -La was 95.67 ± 1.04% and purity of α -La was 98.78 ± 1.19%. UCON was recovered by a thermally-induced phase separation and reused in next ATPF process without reducing E of α -La. Purified α -La was characterized by several key technologies. The results indicated that α -La in cow milk whey could be directly separated and purified by the ATPF and the purity was satisfactory. Moreover, it was suggested there was no obvious structure difference between the α -La separated by ATPF and the α -La standard. CONCLUSION The present study enabled the recycling of UCON, providing an effective, economically viable and environmentally friendly approach for the separation and purification of protein. © 2021 Society of Chemical Industry
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BIOMEDICAL CHROMATOGRAPHY
·
2025
·Vol. 40
·期 1
·e70320
·
IF 1.7
Ying Ma,Yu Yao,Yue Pu,Hong Chen,Juan Zhang
Parkinson's disease (PD) is a progressive neurodegenerative disorder marked by the loss of dopaminergic neurons in the substantia nigra and the pathological accumulation of α-synuclein (α-Syn). Rotenone, a mitochondrial complex I inhibitor, reliably reproduces these hallmark features and is therefore widely used to establish experimental PD models. Shiliuwei Kangchanzhijing capsules (SLW-KCZJ capsules), a traditional Chinese medicine (TCM) formulation comprising 16 herbal components, were specifically developed for PD management. This study systematically evaluated the neuroprotective effects and underlying mechanisms of SLW-KCZJ in a rotenone-induced PD mouse model. SLW-KCZJ treatment significantly improved movement distance and balance–coordination, attenuated neuronal loss in the substantia nigra, and markedly suppressed neuroinflammatory responses. At the molecular level, the capsules increased tyrosine hydroxylase expression by approximately 200% and elevated Parkin and protein phosphatase 2A levels by approximately 60% and 80%, respectively. In contrast, they robustly inhibited polo-like kinase 2 expression and reduced α-Syn levels by approximately 60% and 40%. The intervention also increased striatal dopamine (DA) and homovanillic acid levels while lowering neurofilament light chain and oligomeric α-Syn. In summary, this study provides the first evidence that SLW-KCZJ capsules exert neuroprotective effects by modulating the Parkin/PP2A/α-Syn signaling pathway, offering a promising TCM-based therapeutic strategy for PD.
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MICROCHIMICA ACTA
·
2017
·Vol. 184
·期 10
·3901-3907
·
IF 4.58
Miao Peng,Yang Dawei,Chen Xifeng,Guo Zhenzhen,Tang Yuguo
The authors are presenting an electrochemical aptasensor for tumor necrosis factor-alpha (TNF-α) detection that is aided by the use of magnetic nanoparticles (NPs) and two DNA probes. Firstly, magnetic NPs coated with gold NPs (Fe 3 O 4 @AuNP) are synthesized. Then, DNA probe 1 with a terminal thiol group is immobilized on the surface of Fe 3 O 4 @AuNP via gold-thiol chemistry. DNA probe 1 is then hybridized with DNA probe 2, which is labeled with Methylene Blue (MB). The composite of Fe 3 O 4 @AuNP-DNA duplex is formed as a result, which can be easily absorbed on a magnetized glassy carbon electrode. The electrochemical signal is obtained after reductant-mediated amplification. Since MB-labeled DNA probe 2 is the aptamer against TNF-α, it will be released in the presence of TNF-α. This process leaves Fe 3 O 4 @AuNP-DNA probe 1 on the surface of the electrode. Thus, the recorded electrochemical response decreases dramatically. The assay, best operated at a working voltage of −0.39 V (vs. SCE), has a linear response in the 10 pg mL −1 to 100 ng·mL −1 TNF-α concentration range, and the limit of detection is 10 pg mL −1 . The sensing strategy is highly sensitive and selective, and has been successfully applied to real samples.
Schematic of the biosensor. The composite of Fe 3 O 4 @AuNP-DNA duplex is placed on a glassy carbon electrode. After interaction between TNF-α and its aptamer, DNA probe is released with the labeled electrochemical species. The decrease in electrochemical response serves as the analytical signal.
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BIORESOURCE TECHNOLOGY
·
2020
·
IF 7.539
Lian Xu,Li-Chao Wang,Bing-Mei Su,Xin-Qi Xu,Juan Lin
L-threonine transaldolase (PsLTTA) could asymmetric synthesize β-hydroxy-α-amino acids (HAAs) with excellent stereoselectivity, while the poor yield limited its further application. Here we provided a combinatorial strategy to improve HAAs production, by directed evolution of PsLTTA towards enhanced activity and introducing an acetaldehyde elimination system to avoid acetaldehyde over-accumulation. A novel high throughput screening (HTS) method for evaluating PsLTTA activity was developed and applied for directed evolution of PsLTTA. Subsequently, we co-expressed alcohol dehydrogenase and formate dehydrogenase to construct an acetaldehyde elimination system to remove acetaldehyde inhibition. Moreover, the above positive strategies were integrated. As a result, the (2S,3R)- p -methylsulfonyl phenylserine yield reached 154.0 mM and with 94.6% de value, the highest productivity and stereoselectivity of (2S,3R)-HAAs reported by enzymatic synthesis so far. Taken together, our studies provided an efficient and green route for chiral synthesis of (2S,3R)-HAAs, which might contribute to the industrialization production of these useful building blocks.
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JOURNAL OF ALLOYS AND COMPOUNDS
·
2024
·
IF 5.8
Huajie Lan,Lei Tian,Wenfeng Wang,Yuan Li,Shumin Han,Lu Zhang
The currently used β -Ni(OH) 2 for alkaline nickel batteries are falling short of higher capacity demand. In contrast, α -Ni(OH) 2 with high theoretical capacity can address this need. However, the α -Ni(OH) 2 suffers from poor stability and intrinsic low electrical conductivity. Herein, we designed La/Ce-doped spherical α -Ni(OH) 2 and study the effect of La/Ce on the structure and electrochemical properties of α -Ni(OH) 2 . The increased specific surface area of the α -Ni(OH) 2 with La/Ce doping reduces the electron transfer resistance. The La/Ce-doped α -Ni(OH) 2 with 2 at% La (Ni-La-2) and 3 at% Ce (Ni-Ce-3) doping deliver high capacities of ∼365 mAh g –1 and ∼373 mAh g –1 , respectively, which is only 309 mAh g –1 for the pure α -Ni(OH) 2 . Meanwhile, the La/Ce-doping stabilizes the structural robustness of the α -Ni(OH) 2 during cycling. Notably, Ni-La-2 shows a superior stable cycling life with a capacity retention of ∼95 % after 400 cycles, which is ∼20 % higher than that of pure α -Ni(OH) 2 .
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JOURNAL OF MEMBRANE SCIENCE
·
2020
·
IF 7.183
Xiaotai Zhang,Hai Huang,Xiaoming Li,Jian Wang,Yangyang Wei,Huifeng Zhang
Active chlorine-vulnerability is the Achilles' heel for polyamide thin film composite (PA-TFC) reverse osmosis (RO) membranes during water desalination and/or purification. Chlorination disrupts the delicate PA skin layer on porous support, resulting in significantly deteriorative membrane permselectivity and rising maintenance cost. Inspired by the superior antioxidative capacity of biologically endogenous α-lipoic acid (LA), this work was dedicated to tailoring a chlorine-resistant RO membrane with LA sacrificial unit on the surface. It was evidenced that the characteristic dithiolane within LA preferentially reacted with active chlorine in feedwater, and the intimal PA selective layer was thus safeguarded from chlorine-triggered oxidation . The modifier was pre-assembled with LA and polyethylenimine (PEI) as the mediator, subsequently tethered onto membrane surface via one-step chemical coupling without any catalysts. A pretty balance between membrane permselectivity and grafted modifier amount was achieved via regulating the modifier concentration. The modified membrane exhibited significantly ameliorative chlorine-resistance relative to the pristine one, with normalized salt rejection still remained (99.3 ± 0.6)% and only (17 ± 5)% final flux loss after a rigorous static chlorine exposure of 8000 ppm·h under acidic condition (confidence level, P = 95%). The electronic energy differences of chlorination-involved chemical procedures were determined by density functional theory (DFT) calculation, and an energy-favored three-stage tandem oxidation mechanism of LA moiety was proposed. Accordingly, up to 5 equiv. of active chlorine can be captured by a single sacrificial unit. Such intrinsically high chlorine-consumption efficacy of LA-based sacrificial layer would retard the chlorination saturation that commonly invalidates sacrificial layers. This bioinspired work shed light on a novel, facile, and effective anti-chlorine strategy for PA-TFC RO membrane to maintain the stability of service performance.
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IUCrJ
·
2021
·
IF 4.769
Jiawei Lin,Peng Shi,Ying Wang,Lingyu Wang,Yiming Ma,Fei Liu,Songgu Wu,Junbo Gong
Template design on polymorph control, especially conformational polymorphs, is still in its infancy and the result of polymorph control is often accidental. A method of regulating the crystallization of conformational polymorphs based on the crystal structure similarity of templates and the target crystal form has been developed. Crystal structure similarity was considered to be able to introduce lattice matching (geometric term) with chemical interactions to regulate conformational polymorph nucleation. The method was successfully applied to induce the crystallization of DA7-II [HOOC–(CH 2 ) n −2 –COOH (diacids), named DA n , where n = 7, 9, 15, 17 and II represents the metastable polymorph] on the surface of DA15-II. An analogous two-dimensional plane – the (002) face of both DA15-II and DA7-II – was firstly predicted as the epitaxially attached face with similar lattice parameters and the strongest adsorption energy. The powder DA15-II template with the preferred orientation face in (002) presented much stronger inducing DA7-II ability than the template with other preferred orientation faces. The epitaxial growth of DA7-II on DA15-II through an identical (002) face was clearly observed and verified by the single-crystal inducing experiments. The molecular dynamics simulation results demonstrated that the strong interactions occurred between DA7 molecules and the (002) face of DA15-II. This method has been verified and further applied to the crystallization of DA7-II on the surface of DA17-II and DA9-II on the surface of DA15-II. This study developed a strategy based on structure similarity to regulate the conformational polymorph and verified the significant role of lattice matching and chemical effects on the design and preparation of templates.
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