ACS Catalysis
·
2024
·
IF 11.3
Yuexiang Li,Junliang Liu,Shuqi Li,Shaoqin Peng
Hydrogen production through electrochemical water splitting (EWS) presents a viable solution for addressing the fossil energy crisis. However, the commercial viability of this approach is impeded by the sluggish kinetics of the oxygen evolution reaction (OER). It is urgently needed to develop efficient, stable, and cost-effective OER electrocatalysts. Herein, we comprehensively design and investigate a phosphate ion and Fe3+ codecorating Ni(OH)2/NiOOH electrocatalyst (Pi-Fe:NiOH) for OER. This codecoration induces multiple synergistic effects, which include an increase in the interlayer water content for the internal OER, altering the OER mechanism, facilitating proton transport across the layers, and improving the stability of Pi-Fe:NiOH. Consequently, Pi-Fe:NiOH exhibits a high OER activity with overpotentials of 118 ± 1 and 222 ± 4 mV at current densities of 10 and 100 mA cm–2, respectively. More impressively, it maintains stable operation at a high current density of around 300 mA cm–2 for at least 500 h, much better than the Ni(OH)2/NiOOH electrocatalyst (NiOH) for less than 6 h at a current density below 200 mA cm–2. These findings offer insights for the design of anion–cation codoped hydroxide eletrocatalysts, paving a way for the development of efficient and stable OER electrocatalysts.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Jianing Fu,Shaobo Li,Meizhen Xu,Ling Liu,Li Chen,Dequan Zhang
Soup is an important presence in diet, but its absorption and transport mechanism by the human body remains unclear. In this study, Caco-2 intact cell and monolayer cell models were constructed to simulate small intestine absorption on colloidal nanoparticles (CNPs) isolated from lamb soup. The intracellular localization of CNPs was viewed by laser confocal microscopy (LSCM). CNPs uptake and release pathways were explored by differences in CNPs concentrations in 5 endocytosis inhibitor models and 4 exocytosis inhibitor models. Results indicated that CNPs endocytosis by Caco-2 cells was restrained by Nystatin and Cytochalasin D, with exocytosis being inhibited by Nocodazole and Monensin. Therefore, the major absorption pathways for CNPs were caveolin-dependent endocytosis, macropinocytosis and phagocytosis. The major transport pathways were microtubule-vesicle-mediated protein transport to the membrane and transportation between the Golgi apparatus and membrane. This study may provide theoretical support for the transport mechanism of soup products in the small intestine.
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ZEITSCHRIFT FUR ANORGANISCHE UND ALLGEMEINE CHEMIE
·
2024
·
IF 1.1
Zhiwei Zhang,Yilin Ma,Encheng Liu,Junjie Cheng,Fengfeng Chen,Bin Ling,Ningyu Di,Junkuo Gao
Curing agents for epoxy resin (EP) are one of the most important branches in the polymer materials field due to their enormous potential in many fields, but constructing them in a well-controlled curing temperature remains challenging. Herein, a novel curing agent is constructed by the zirconium metal-organic framework (UiO-66) and [P 4442 ] 2 [IDA] through an impregnation method. [P 4442 ] 2 [IDA]@UiO-66 is utilized as a functional nanofiller and curing agent to construct the [P 4442 ] 2 [IDA]@UiO-66/EP nanocomposites. The chemical structures of [P 4442 ] 2 [IDA]@UiO-66 were investigated by XRD, SEM, DSC, and TG characterizations. The various [P 4442 ] 2 [IDA]@UiO-66 with different loading amounts of [P 4442 ] 2 [IDA] were used to investigate the curing behaviors of the EP-51. The DSC results confirm that the curing temperature of the [P 4442 ] 2 [IDA]@UiO-66/EP is higher than that of [P 4442 ] 2 [IDA]/EP. In addition, the kinetic parameters and the activation energy of the curing reaction were acquired according to the fitting of Kissinger equation and Ozawa equation. The drying time of [P 4442 ] 2 [IDA]@UiO-66/EP system at 40 °C was enhanced 17 times compare to that of [P 4442 ] 2 [IDA]/EP system. This study opens new avenues for the rational design of EP nanocomposites by employing ionic liquids@MOFs composite materials as curing agent for wide engineering applications.
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Synthetic and Systems Biotechnology
·
2024
·
IF 4.4
Shanquan Liang,Zi He,Dan Liu,Shaoqing Yang,Qiaojuan Yan,Zhengqiang Jiang
2′-Fucosyllactose (2′-FL) is one of the important functional oligosaccharides in breast milk. So far, few attempts on biosynthesis of 2′-FL by the salvage pathway have been reported. Herein, the salvage pathway enzyme genes were introduced into the E. coli BL21star(DE3) for synthesis of 2′-FL. The 2′-FL titer increased from 1.56 to 2.13 g/L by deleting several endogenous genes on competitive pathways. The α-1,2-fucosyltransferase (WbgL) was selected, and improved the 2′-FL titer to 2.88 g/L. Additionally, the expression level of pathway enzyme genes was tuned through optimizing the plasmid copy number. Furthermore, the spatial distribution of WbgL was enhanced by fusing with the MinD C-tag. After optimizing the fermentation conditions, the 2′-FL titer reached to 7.13 g/L. The final strain produced 59.22 g/L of 2′-FL with 95% molar conversion rate of lactose and 92% molar conversion rate of fucose in a 5 L fermenter. These findings will contribute to construct a highly efficient microbial cell factory to produce 2′-FL or other HMOs.
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Scientific Reports
·
2017
·Vol. 7
·期 1
·1-10
·
IF 4.259
Yang Li,Cai Tianming,Ding Dahu,Cai Tianjin,Jiang Canlan,Li Hua,Yang Qian,Chen Liwei
2-hydroxy-1,4 naphthoquinone (lawsone) is widely used and induces environmental pollutions during its production and application. In the present study, a lawsone-degrading bacterium strain, LH-3 was successfully isolated from the activated sludge. Based on the 16S rRNA gene analysis, the strain LH-3 phylogenetically belonged to the Pseudomonas taiwanensis . It could degrade 200 mg L −1 lawsone completely in 9 h with an inoculum quantity of 1% (v/v). The effects of environmental conditions on the degradation process and the degradation pathway were systematically investigated. LH-3 could maintain its high degradation efficiency under high salt condition. The identified intermediates of salicylic acid, 2-hydroxy-4-oxo-chroman-2-carboxylic acid, and catechol elucidated the potential degradation pathway. Furthermore, the immobilized LH-3 strain cells prepared with alginate gel and biochar performed excellent stability in nine successive degradation runs. It could sucessfully survive in laboratory scale sequencing batch reactor and become to be the dominant species. This study clearly revealed that LH-3 could serve as an attractive candidate for the microbial remediation of lawsone-containing wastewater.
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RSC Advances
·
2016
·Vol. 6
·期 53
·47966-47973
·
IF 3.289
Yao Shi,Xiaoliang Zhang,Yongmei Zhu,Hongliang Tan,Xiangshu Chen,Zhang-Hui Lu
Ag–CeO2 nanocomposites (Ag@CeO2 NCs) with a core–shell structure have been successfully synthesized through the combination of a redox reaction and the reverse micelle technique without any additional reductants or surfactants. Under a N2 atmosphere, the redox reaction automatically occurs between Ag+ and Ce3+ in an alkaline solution, resulting in the formation of Ag@CeO2 NCs by the self-assembly process. By using the XRD, FE-SEM, TEM, XPS, and ICP methods, the characterized results of Ag@CeO2 NCs show that Ag nanoparticles (NPs) with a diameter of 3–7 nm are surrounded by CeO2 NPs. Compared to CeO2 supported Ag NPs, free Ag NPs, and CeO2, the as-synthesized Ag@CeO2 NCs exhibit a superior catalytic activity and high sustainability for the hydrogenation of 4-nitrophenol (C6H5NO3, 4-NP) and 2-nitroaniline (C6H6N2O2, 2-NA) with sodium borohydride (NaBH4) in water solution at room temperature. The Ag@CeO2 NCs can achieve a complete reduction of 4-NP and 2-NA with turnover frequencies (TOF) of 138.7 and 109.8 h−1, respectively. The durability tests show that the Ag@CeO2 NCs are still highly active for the reduction of 4-NP and 2-NA, preserving 98% and 95% of their initial catalytic activity even after ten runs.
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RSC Advances
·
2016
·Vol. 6
·期 41
·34081-34089
·
IF 3.289
Liang Zhao,Kai Zhang,Wenhuan Bu,Xiaowei Xu,Han Jin,Bei Chang,Banchao Wang,Yingjian Sun,Bai Yang,Changyu Zheng,Hongchen Sun
Treating bone defects is still a challenge in clinical practice. Recently, researchers used human bone morphogenetic protein 2 gene (hBMP-2) to induce osteoblast differentiation and promote new bone formation. However, an efficient way to deliver hBMP-2 still needs to be created. In this study, we evaluated whether chitosan–polyethylenimine (CS–PEI) nanoparticle could effectively deliver hBMP-2 locally with lower or no toxicity and promote osteoblast differentiation and new bone formation in vitro and in vivo. Data demonstrated that the synthesized CS–PEI/hBMP-2 nanoparticle at a W/W ratio of 20 to 1, which was the smallest size (162 nm) and highest zeta potential (24 mV), effectively transfected MC3T3-E1 cells without cytotoxicity in vitro, and had the ability to promote cell proliferation. Interestingly, the CS–PEI/hBMP-2 nanoparticle eliminated disadvantages of lower transfection efficiency from chitosan and cytotoxicity from PEI. RT-QPCR data showed that MC3T3-E1 cells treated with CS–PEI/hBMP-2 nanoparticle dramatically expressed higher levels of BMP-2 and significantly increased gene expressions of Col1 on days 3 and 14, Sp7 on days 3, 7, and 14, and ALP on day 14. Alizarin red staining demonstrated that CS–PEI/hBMP-2 nanoparticle-treated MC3T3-E1 cells significantly increased cell mineralization. These in vitro data suggest that the CS–PEI/hBMP-2 nanoparticle can effectively induce osteogenic differentiation of MC3T3-E1 cells in vitro. Western blot analysis further demonstrated that transgene BMP-2 indeed phosphorylated Smad1/5/8, which indicates that CS–PEI/hBMP-2 nanoparticle affects cell differentiation through a BMP-2 signal pathway. Importantly, in vivo data showed that CS–PEI/hBMP-2 nanoparticle clearly promoted new bone formation at the bone defect area 12 weeks post-implantation. This indicates that synthesized CS–PEI/hBMP-2 nanoparticle has the potential to become a useful therapeutic vector for bone defect treatment with further modification.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2017
·
IF 3.154
Hongbao Lu,Shuangyu Xu,Wenjuan Zhang,Chunmei Xu,Beixing Li,Daxia Zhang,Wei Mu,Feng Liu
Botanical nematicides have recently received increasing interest because of the high risks of some traditional nematicides to human health and the environment. This study evaluated the nematicidal activity of a plant volatile, trans-2-hexenal, against Meloidogyne incognita. This compound exhibited higher activity in a fumigation experiment than in the aqueous phase in vitro. Both in pot tests and in field trials, trans-2-hexenal showed significant efficacy against M. incognita while maintaining excellent plant growth, especially at doses of 1000 and 500 L ha–1, which were superior to that of abamectin at 180 g ha–1 via hole application treatment but not significantly different from fumigation with 400 kg ha–1 of dazomet. Furthermore, plants treated with 500 L ha–1trans-2-hexenal had fruit yields 20.2 and 45% greater than the control group. On this basis, trans-2-hexenal may be a potential alternative fumigation agent for controlling M. incognita on tomato crops.
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ZEITSCHRIFT FUR ANORGANISCHE UND ALLGEMEINE CHEMIE
·
2016
·Vol. 642
·期 4
·343-349
·
IF 1.261
Xiao Zhao,Dajiang Mei,Jingli Xu,Yuandong Wu
Three compounds A Sb 2 (SO 4 ) 2 (PO 4 ) ( A = H 3 O + , K, Rb) were obtained from the reactions of Sb 2 O 3 , A 2 CO 3 ( A = Li, Rb) or K 2 SO 4 and NH 4 H 2 PO 4 in H 2 SO 4 (98 %) at 220–250 °C. Their structures were determined by single-crystal X-ray diffraction. All compounds crystallize in the triclinic space group P (no.2) and are isostructural. The crystal structures consist of two-dimensional 2 ∞ [Sb 2 (SO 4 ) 2 (PO 4 )] – anionic layers and alkali cations, which are located between anionic layers. The anionic layers are composed of [SbO 4 ] ψ-trigonal bipyramids, [SbO 5 ] ψ octahedra, [SO 4 ] tetrahedra, and [PO 4 ] tetrahedra. All compounds are characterized by solid state UV/Vis/NIR diffuse reflectance spectra, FT-IR spectroscopy, and Raman spectroscopy.
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International Journal of Environmental Research and Public Health
·
2016
·Vol. 13
·期 11
·1085
·
IF 2.035
Yiyang Jia,Te Liu,Liting Zhou,Jian Zhu,Juan Wu,Di Sun,Jin Xu,Qi Wang,Huaiji Chen,Feng Xu,Yuezhu Zhang,Tianrong Zhang,Hongbo Liu,Lin Ye
The most widely used plasticizer, di-(2-ethylhexyl) phthalate (DEHP), is known to affect lipid metabolism and adipogenesis. We studied the effects of dietary DEHP exposure on metabolism in rats as well as the role of the JAK/STAT pathway in this process. Eighty rats were exposed to DEHP (0, 5, 50 and 500 mg/kg/d) through dietary intake for 4 weeks. We then collected blood samples, liver, and adipose tissues to detect modifications in the levels of serum lipids, leptin, adiponectin and insulin. JAK3, STAT5a and PPARγ expression were detected at both the gene and protein levels. The activation of JAK3 and STAT5a was also detected. The DEHP-exposed rats had increased body weight, serum lipid, insulin, and leptin levels. Moreover, the JAK3/STAT5a pathway was activated in the adipose tissue; however, this pathway was not activated in the liver. The mRNA of SREBP-1c in the liver was increased significantly among each of the groups, in contrast to the levels found in the mature SREBP-1c protein form. Furthermore, the expression ofFABP4,AcoxandFASnwas decreased in the liver, but increased in adipose tissue. Thus, we conclude that exposure to DEHP reduces the hydrolysis of lipid and promotes triglyceride accumulation by oppositely regulating the activation state of JAK/STAT pathway in the liver and adipose tissue, resulting in the disorder of body lipid metabolism and obesity.Keywords:di-(2-ethylhexyl) phthalate;JAK/STAT;lipid metabolism;adipose tissue
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MATERIALS RESEARCH BULLETIN
·
2016
·
IF 2.435
Liangjun Zhou,Wenxi Wang,Sicen Yu,Bo Nan,Yinggang Zhu,Yang Shi,Haohong Shi,Xingzhong Zhao,Zhouguang Lu
Single-phase and Dy 3+ /Eu 3+ co-doped novel phosphor LiY(MoO 4 ) 2−x (WO 4 ) x :Dy 3+ ,Eu 3+ , emitting white luminescence under near ultraviolet (NUV) light, was prepared through sol-gel method. The preparation and characterization of this phosphor were systematically studied by X-ray diffraction and spectrofluorophotometric measurements. The molar ratio of metal ions and citric acid (R m/c ) and calcination temperature greatly influenced the phase purity of sample, revealing that the pure phase could be obtained by using R m/c = 1:2.5 and calcination temperature = 800 °C. Under NUV excitation, the as-prepared phosphor exhibited the emissions of 485, 572 and 612 nm, which intensities could be affected by the pH and the concentrations of molybdenum and tungsten ions. By doping appropriate concentrations of Dy 3+ and Eu 3+ , white light emitting phosphor LiY(MoO 4 ) 1.2 (WO 4 ) 0.8 :6%Dy 3+ ,7%Eu 3+ with good responsiveness to NUV light was obtained. The Commission Internationale de L’Eclairage chromaticity coordinates were calculated to be (x = 0.334, y = 0.322), close to the D 65 illuminant (x = 0.313, y = 0.329), indicating the potential application for NUV WLEDs.
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ADVANCED SYNTHESIS & CATALYSIS
·
2016
·Vol. 358
·期 18
·2923-2928
·
IF 6.453
Peiyuan Yao,Yunfeng Cui,Shanshan Yu,Yuncheng Du,Jinhui Feng,Qiaqing Wu,Dunming Zhu
An efficient multi-enzyme cascade reaction for the synthesis of ( R )- or ( S )-2-hydroxybutyric acid [( R )- or ( S )-2-HB] from l -threonine was developed by using recombinant Escherichia coli cells expressing separately or co-expressing l -threonine deaminase from Escherichia coli K-12 ( ilvA ), formate dehydrogenase (FDH) from Candida boidinii and l -lactate dehydrogenase ( l -LDH) from Oryctolagus cuniculus or d -lactate dehydrogenase ( d -LDH) from Staphylococcus epidermidis ATCC 12228. Up to 750 mM of l -threonine were completely transformed to ( R )- or ( S )-2-HB in optically pure form (>99% ee ) with high isolated yields. This one-pot multi-enzyme transformation provides a new practical method for the synthesis of these important optically pure compounds.
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