INDUSTRIAL CROPS AND PRODUCTS
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2021
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IF 5.645
Yijuan Chen,Jiaxuan Luo,Nan Zhang,Wenjuan Yu,Jiexian Jiang,Guanghui Dai
Plant essential oils are the powerful candidates to develop natural insect management agents. Their chemical compounds along with their biological activity are often affected by many factors. However, the relationships between the composition and bioactivity of the compounds are not well understood. In this study, eight plant essential oils were screened, and the main compounds from the most effective oil showing insecticidal properties against third-instar larvae of the beet armyworm ( Spodoptera exigua Hübner) were evaluated for comparative and synergistic interactions by topical application. The results indicated that Salvia hispanica L. essential oil showed significant insecticidal activity against S. exigua compared with the other seven essential oils. Fifteen major compounds were identified from S. hispanica via gas chromatography-mass spectrometry, with the most abundant compound (α-thujone) possessing the highest insecticidal activity. Several combinations of the four major compounds (α-thujone, (+)-camphor, 1,8-cineole, and α-caryophyllene) from S. hispanica exhibited synergistic insecticidal activities. This study demonstrates the potential of S. hispanica essential oil and α-thujone as effective botanical insecticides in the control of beet armyworm and provides a better understanding of essential oil compound-pest interactions.
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MICROCHEMICAL JOURNAL
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2021
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IF 4.821
Yuanyuan Niu,Jianfeng Zhou,Haohong Lai,Qing Zhou,Shumei Wang,Haiyun Zhai
A sensitive and selective electrochemical sensor for sensing 4-nitroquinoline N-oxide (4-NQO) by differential pulse voltammetry was designed. Poly L-histidine (P-L-his) composite and metal-organic framework UiO-66 (Zr) modified electrode was used as the working electrode which had large specific surface area, mesoporous structure and excellent conductivity. Unlike most modification methods, UiO-66 was electrodeposited in this study. Compared with drop coating, electrodeposition proceeds efficiently under mild conditions and often requires shorter time. FTIR, EDX, TEM, SEM and electrochemical assay were used to characterize the prepared materials and modified electrodes. After that, some experimental conditions like electro-polymerization time, deposition time, pH and scan rate were also improved and optimized separately. At the UiO-66/P-L-his-modified glassy carbon electrode, a good linear relationship between 4-NQO concentration and its peak current was obtained under the best conditions in a wide range from 0.2 to 50.0 μM, and the limit of detection (S/N = 3) was 66.7 nM. Moreover, multiple experiments elucidated that the as-synthesized sensor could be applied to detect 4-NQO in actual samples, and the recoveries results obtained were satisfactory.
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COLLOIDS AND SURFACES B-BIOINTERFACES
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2021
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IF 5.268
Yujie Chen,Zihao Jia,Muhammad Shafiq,Xianrui Xie,Xianghao Xiao,Rita Castro,João Rodrigues,Jinglei Wu,Guangdong Zhou,Xiumei Mo
Electrospun nanofibers emulate extracellular matrix (ECM) morphology and architecture; however, small pore size and tightly-packed fibers impede their translation in tissue engineering. Here we exploited in situ gas foaming to afford three-dimensional (3D) poly(L-lactide-co-ε-caprolactone)/silk fibroin (PLCL/SF) scaffolds, which exhibited nanotopographic cues and a multilayered structure. The addition of SF improved the hydrophilicity and biocompatibility of 3D PLCL scaffolds. Three-dimensional scaffolds exhibited larger pore size (38.75 ± 9.78 μm 2 ) and high porosity (87.1% ± 1.5%) than that of their 2D counterparts. 3D scaffolds also improved the deposition of ECM components and neo-vessel regeneration as well as exhibited more numbers of CD163 + /CCR7 + cells after 2 weeks implantation in a subcutaneous model. Collectively, 3D PLCL/SF scaffolds have broad implications for regenerative medicine and tissue engineering applications.
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INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2021
·Vol. 56
·期 12
·6591-6600
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IF 3.713
Jiali Yang,Tengfei Wang,Jianbing Di,Yaping Liu,Xiaoling Hao,Yu Wang
Summary Although several studies have shown the ability of l -glutamate (glutamate) to mitigate the stress imposed by pathogens, the underlying mechanism is still in its infancy. To gain further knowledge, this study focussed on the effect of glutamate on primary nitrogen and carbon metabolisms during apple- Penicillium expansum interaction. The obtained result showed that glutamate could effectively restrict blue mould rot development in apples, but had no direct impact on fungal growth in vitro . The application of glutamate increased the level of nitrogen in apples, resulting in a disrupted balance of carbon and nitrogen. Consistently, the key enzymes glutamine synthetase (GS) and glutamate synthase (GOGAT) involved in the GS/GOGAT cycle, and the deaminating activity of glutamate dehydrogenase (GDH), forming 2-oxoglutarate and ammonium from glutamate, were promptly stimulated by glutamate. Interestingly, glutamate led to considerable consumption of the tricarboxylic acid (TCA) cycle intermediates, such as isocitric acid and citric acid, accompanied by the enhancement of malate dehydrogenase and succinate dehydrogenase activities. Collectively, exogenous application of glutamate might confer blue mould resistance in apples, at least in part, by redirecting host’s primary nitrogen and carbon metabolisms, such as the activation of the GS/GOGAT cycle, deaminating activity of GDH and the TCA cycle.
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MICROCHIMICA ACTA
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2021
·Vol. 188
·期 6
·1-8
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IF 5.833
Wang Hai-Bo,Mao An-Li,Tao Bei-Bei,Zhang Hong-Ding,Xiao Zhong-Liang,Liu Yan-Ming
An interesting phenomenon is described that the fluorescence signal of poly(adenine) (A) DNA-templated gold nanoclusters (AuNCs) is greatly improved in the presence of l -histidine by means of l -histidine-DNA interaction. The modified nanoclusters display strong fluorescence emission with excitation/emission maxima at 290/475 nm. The fluorescence quantum yield (QY) is improved from 1.9 to 6.5%. Fluorescence enhancement is mainly ascribed to the l -histidine-DNA interaction leading to conformational changes of the poly(A) DNA template, which offer a better microenvironment to protect AuNCs. The assay enables l -histidine to be determined with good sensitivity and a linear response that covers the 1 to 50 nM l -histidine concentration range with a 0.3 nM limit of detection. The proposed method has been applied to the determination of imidazole-containing drugs in pharmaceutical samples. Graphical abstract
A turn-on fluorescent method has been designed for the sensitive detection of l -histidine as well as imidazole-containing drugs on the basis of the l -histidine-DNA interaction.
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FOOD CHEMISTRY
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2021
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IF 7.514
Xin Tong,Jiehong Yang,Yu Zhao,Haofang Wan,Yu He,Ling Zhang,Haitong Wan,Chang Li
Natural deep eutectic solvents (NaDESs) are promising green alternatives to conventional solvents widely applied in the extraction of natural products due to their physical and chemical superiorities. In present study, 22 NaDESs consisted from food grade ingredients were screened in ultrasonic assisted extraction (UAE) of bioactive compounds from safflower. The oral bioavailabilities of hydroxysafflor yellow A (HSYA) and anhydrosafflor yellow B (ASYB) in the extracts were then investigated in SD rats with the help of HPLC-MS technique. The results revealed that l -proline-acetamide ( l -Pro-Am) was an effective solvent with the yields of HSYA and ASYB at 32.83 and 8.80 mg/g. Pharmacokinetic studies revealed that the blood level of HSYA and ASYB were significantly higher after oral administration of l -Pro-Am extract than that of aqueous extract. Especially, the relative bioavailabilities (to aqueous extract) of HSYA and ASYB were calculated 183.5% and 429.8%.
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DESALINATION
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2020
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IF 7.098
Chenpu Wu,Quanling Xie,Zhuan Hong,Lufang Shen,Tong Yu,Honghui Guo,Ying Xiong,Guoliang Zhang,Yinghua Lu,Wenyao Shao
To fabricate thin-film nanocomposite (TFN) nanofiltration (NF) membrane with enhanced desalination and antifouling performance, L-aspartic acid functionalized graphene quantum dots (AGQDs), integrating the advantages of L-aspartic acid and graphene quantum dots (GQDs), were successfully synthesized and incorporated into the polyamide (PA) selective layer via interfacial polymerization (IP) method. The fabricated TFN membranes with various AGQDs contents were systematically characterized by the microstructures, surface properties, desalination, and antifouling performance. The optimal AGQDs concentration in the aqueous solution reaches 3000 ppm, the resultant TFN-3 membrane exhibits an outstanding water permeance of 18.5 LMH/bar, 60.9% higher than the TFC-blank membrane. Meanwhile, the TFN-3 membrane possesses a higher Na 2 SO 4 rejection than that of the TFC-blank membrane (98.4% vs 95.7%). Additionally, the TFN-3 membrane shows superior antifouling performance over the TFC-blank membrane in the presence of either the negatively charged foulant (BSA) or the positively charged foulant (lysozyme). An appropriate amount of AGQDs added into aqueous solution favors the formation of a smooth, thin, hydrophilic , and electrically neutral selective layer. As a result, the overall separation performance of the TFN-3 membrane, especially the desalination and antifouling performance, are significantly enhanced with the help of AGQDs. Therefore, the incorporation of amino acid-functionalized GQDs into the PA selective layer presents a novel strategy to produce a high-performance NF membrane for water desalination.
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CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
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2020
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IF 0.936
Zhi-Kun BAI,Yi-Tao ZHANG,She-Hong LI,Hong-Xia LUO
A flexible graphene platform electrode (GPE) was prepared by transferring chemical vapor deposition graphene film from Cu foil to polyethylene terephthalate substrate with the help of polymethyl-methacrylate. L -Arginine ( L -Arg) was electrodeposited on GPE to form a poly( L -Arg)/GPE (P( L -Arg)/GPE) which was characterized by scanning electron microscopy, energy dispersive spectroscopy and Raman spectroscopy. The electrocatalytic activity and enhanced conductivity of P( L -Arg)/GPE sensor was evaluated by determining xanthine (XA) in the presence of uric acid (UA) and hypoxanthine (HX) in phosphate buffer solution. The electrochemical behaviors of XA on P( L -Arg)/GPE were studied by cyclic voltammetry and differential pulse voltammetry in comparison with those on bare GPE. The proposed sensor not only achieved the selective determination of XA, but also separated the voltammetric signals of UA and HX with potential differences of 420 and 384 mV, respectively. Under the optimized conditions, the proposed sensor displayed superb sensing property towards XA in a wide linear range of 0.5-140 μmol/L with corresponding detection limit of 0.083 μmol/L ( S/N = 3). With good stability, reproducibility, high sensitivity, low cost as well as high resistance to interferences, the P( L -Arg)/GPE had been applied to determine XA in human serum successfully.
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Animal
·
2020
·
IF 2.4
H.B. Zhou,X.Y. Huang,Z. Bi,Y.H. Hu,F.Q. Wang,X.X. Wang,Y.Z. Wang,Z.Q. Lu
Vitamin A is easily degraded by environmental factors. Therefore, it is very important to add antioxidants during Vitamin A production. In the past, ethoxyquin ( EQ ) was widely used, but recent studies have found that it has potential toxicity. Therefore, in this study, we evaluated the antioxidant activities of 4 antioxidants in vitro : EQ, butylated hydroxytoluene, α-tocopherol and L-ascorbic acid sodium salt (Vitamin C sodium). In vitro experiments showed that Vitamin C sodium had better antioxidant capacity. Then, we explored the effects of different antioxidant types of Vitamin A on the growth performance, immune function and antioxidant capacity of weaned pigs. In total, 288 weaned piglets with an initial mean BW of 8.34 ± 0.02 kg at 30 days old were randomly divided into three groups with four replicates and 24 piglets per replicate for 35 days of feeding. The experimental diets were as follows: i) basal diet without external Vitamin A ( NC ); ii) basal diet supplemented with 12 000 IU/kg EQ Vitamin A and iii) basal diet supplemented with 12 000 IU/kg Vitamin C sodium Vitamin A. On day 36, two pigs from each replicate were selected to collect serum samples. The in vivo results showed that pigs in the EQ Vitamin A and Vitamin C sodium Vitamin A groups had significantly higher final weight and average daily gain ( P < 0.05). During the trial, the levels of IgG and glutathione peroxidase in the EQ Vitamin A and Vitamin C sodium Vitamin A groups were significantly higher than those in the NC group ( P < 0.05), and the malondialdehyde content was significantly lower ( P < 0.05). On the 36th day, the levels of IgA and total antioxidant capacity in the Vitamin C sodium Vitamin A group were significantly higher than those in the EQ Vitamin A and NC ( P < 0.05) groups. Thus, Vitamin C sodium Vitamin A can significantly improve the growth performance, antioxidant capacity and immune function of weaned pigs. Meanwhile, Vitamin C sodium may replace EQ as an antioxidant additive for Vitamin A.
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JOURNAL OF APPLIED POLYMER SCIENCE
·
2020
·Vol. 138
·期 18
·50320
·
IF 2.52
Airong Xu,Fen Wang,Luwei Zhang,Xingmin Xu,Zhihong Xiao,Rukuan Liu
Polymer materials based on fossil resources have brought great convenience to various industries. However, environmental pollution is becoming increasingly conspicuous due to their difficult degradation in nature. Therefore, biodegradable composites are highly desired. In the present work, a simple and feasible preparation approach was presented to fabricate biodegradable and biocompatible composite films including methylcellulose/poly( d , l -lactide-co-glycolide) (MC/PLGA) and methylcellulose/poly(1,4-butylene succinate) (MC/PBS). The effects of MC/PLGA and MC/PBS mass ratios on the morphology, crystalline state, mechanical properties and thermal stability of the composite films were systematically investigated. At the same time, the influence of thermal compression treatment on mechanical properties (tensile strength and elongation) and the cyclic utilization of the composite films were also estimated. The composite films displayed enough good mechanical properties, re-usability and thermal stability.
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2020
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IF 4.872
Tingting Liu,Tongtong Li,Lingyan Zhang,Haolin Li,Shangke Liu,Song Yang,Quanshun An,Canping Pan,Nan Zou
Salicylic acid (SA) is an important signal molecule, regulating oxidative stress response in plants. In this study, we evaluated the influences of SA (1 mg L −1 , 10 mg L −1 and 50 mg L −1 ) on the accumulation of clothianidin (CLO), dinotefuran (DFN) and difenoconazole (DFZ) (5 mg L −1 ) and pesticide-induced (CLO-10 mg L −1 , DFN-20 mg L −1 , and DFZ-10 mg L −1 ) oxidative stress in cucumber plants. Exogenous SA at 10 mg L −1 significantly reduced the half-lives of three pesticides in nutrient solution and prevented the accumulation of pesticides in roots and leaves. And the role of SA in reducing residues was related to the major accumulation sites of pesticides. By calculating the root concentration factor (RCF) and translocation factor (TF), we found that SA at 10 mg L −1 reduced the ability of roots to absorb pesticides and enhanced the translocation ability from roots to leaves. Roots exposed to high concentrations of three pesticides could reduce biomass, low chlorophyll content, increase the accumulation of reactive oxygen species (ROS) and proline, promote lipid peroxidation, and alter the activities of a range of antioxidant enzymes, respectively. Exogenous SA at low concentrations (1 mg L −1 and 10 mg L −1 ) significantly mitigated these negative effects. Hence, application of exogenous SA at 10 mg L −1 could effectively alleviate the accumulation of pesticides and induce stress tolerance in cucumber planting systems.
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BIORESOURCE TECHNOLOGY
·
2021
·
IF 9.642
Jiajie Chen,Rongshuai Zhu,Junping Zhou,Taowei Yang,Xian Zhang,Meijuan Xu,Zhiming Rao
Leucine dehydrogenase (LDH) is widely used in the preparation of L-2-aminobutyric acid (L-2-ABA), however its wide application is limited by 2-ketobutyric acid (2-OBA) inhibition. Firstly, a novel high-throughput screening method of LDH was established, specific enzyme activity and 2-OBA tolerance of Lys72Ala mutant were 33.3% higher than those of the wild type. Subsequently, we constructed a single cell comprised of ivlA , Esldh K72A , fdh and optimized expression through fine-tuning RBS intensity, so that the yield of E. coli BL21/pET28a-R3 ivlA-Esldh K72A -fdh was 2.6 times higher than that of the original strain. As a result, 150 g L-threonine was transformed to 121 g L-2-ABA in 5 L fermenter with 95% molar conversion rate, and a productivity of 5.04 g·L -1 ·h −1 , which is the highest productivity of L-2-ABA currently reported by single-cell biotransformation. In summary, our research provided a green synthesis for L-2-ABA, which has potential for industrial production of drug precursors.
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