BIOCHEMICAL ENGINEERING JOURNAL
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2020
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IF 3.475
Xiaoyang Ou,Fei Peng,Xiaoling Wu,Pei Xu,Minhua Zong,Wenyong Lou
A robust Escherichia coli ( E. coli ) strain, with a dual protection system to fight against microbial contamination and T7 phage infection, is used as a chassis to overexpress various proteins in auxotrophic MOPS medium. Among them, a robust EH (epoxide hydrolase)-producing E. coli was used as a biocatalyst to enantioselectively resolve racemic glycidyl o -methylphenyl ether ( rac - o -GMPE) for preparing ( R )- o -GMPE with 6416.7 U/g wet cells. The optimal temperature, pH and biocatalyst dosage were 25 °C, 7.0 and 0.2 mg/mL in aqueous buffer, respectively. The maximal yield (23.0 %) and ee (99.2 %) were achieved within 12 min in 20 mM rac - o -GMPE. Additionally, 98.0 % ee and 38.7 % yield were observed by cell-supplemental strategy with substrate concentration of 100 mM. Furthermore, to alleviate substrate inhibition and product toxicity toward the cells, a biphasic system (aqueous buffer: butyl acetate = 0.5:1) was developed to catalyze 3 M rac - o -GMPE with the initial reaction rate of 549.7 mmol/L/h, 91.7 % ee , 17.1 % yield and 3.66 g/L/h space-time yield.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2020
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IF 5.162
Vemana Gowd,Naymul Karim,Lianghua Xie,Mohammad Rezaul Islam Shishir,Yang Xu,Wei Chen
Polysaccharides and fruit extracts are applied in dairy products to enhance their nutritional property, but the effects of such formulations on the functions and biological activities are yet to be explored. Therefore, this study was aimed at evaluating the effect of interactions among milk protein (beta-lactoglobulin; BLG), polysaccharides (pectin, P; chitosan, CH), and anthocyanin (pelargonidin-3- O -glucoside; P3G) in improving the bioavailability and biological activity of P3G. After gastrointestinal digestion (GID), the content of free P3G in different model solutions were as follows: P3G-alone (73.59 μg/mL), P3G-P (66.59 μg/mL), P3G-CH (36.72 μg/mL), P3G-BLG (64.92 μg/mL), P3G-P-BLG (64.92 μg/mL), and P3G-CH-BLG (39.61 μg/mL). Less amount of free P3G in model solutions indicated increased complex formation of P3G with protein and/or polysaccharides during GID. These complexes resulted in protection and progressive release of P3G in the gastrointestinal tract. Chitosan exhibited more protection to P3G compared with P and BLG. In addition, α-glucosidase inhibitory activity and ROS scavenging activities of conjugated-P3G samples were potentially augmented after GID. However, the presence of polysaccharides and protein in the model solutions did not show any negative effect on the biological activity of P3G. Thus, pure P3G can be used as a nutritional ingredient in dairy industries.
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PubMed
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FOOD AND CHEMICAL TOXICOLOGY
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2019
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IF 3.775
Congyong Sun,Wenjing Li,Yingkun Liu,Wenwen Deng,Michael Adu-Frimpong,Huiyun Zhang,Qilong Wang,Jiangnan Yu,Ximing Xu
1-O-(4-hydroxymethylphenyl)-α-L-rhamnopyranoside (MPG) is a phenolic glycoside that exists in Moringa oleifera seeds with various health benefits, whereas its hepatoprotective effect is lacking clarification. Herein, MPG was isolated from Moringa oleifera seeds, and its hepatoprotection against CCl 4 -induced hepatotoxicity in L02 cells and ICR mice was investigated. Toxicity studies showed that MPG did not induce significant changes in organ coefficients and histological analysis, as well as exhibited no cytotoxicity. In vitro studies indicated that MPG substantially increased cell viability and intracellular SOD activities, and significantly inhibited LDH leakage in CCl 4 -treated cells. In vivo studies demonstrated that MPG significantly alleviated CCl 4 -induced hepatotoxicity in mice, as indicated by diagnostic indicators of hepatic injury, as well as the histopathological analysis. Moreover, MPG reduced the lipid peroxidation levels and regulated the inflammatory cytokines. Notably, MPG substantially suppressed the significant elevation of ROS production in hepatocytes of mice intoxicated with CCl 4 . Moreover, TUNEL assay demonstrated that MPG obviously inhibited hepatic apoptosis induced by CCl 4 . Altogether, these results suggested that MPG has excellent liver-protecting effects against hepatocytotoxicity induced by CCl 4 in mice and L02 cells, which can be further developed as a valuable functional food additive or drug for the treatment of hepatic injury.
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PubMed
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APPLIED CATALYSIS B-ENVIRONMENTAL
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2014
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IF 6.007
Ruiping Hu,Xin Xiao,Shunheng Tu,Xiaoxi Zuo,Junmin Nan
Novel flower-like heterostructured β-Bi 2 O 3 /Bi 2 O 2 CO 3 microspheres are synthesized by calcining a Bi 2 O 2 CO 3 self-sacrifice precursor for the visible-light photocatalytic degradation of o -phenylphenol (OPP, is a widely used fungicide and preservative agent). The Bi 2 O 2 CO 3 microspheres are firstly prepared under hydrothermal conditions, and then converted to Bi 2 O 3 by thermal treatment. With increasing the calcining temperature from 250 to 500 °C, an in situ stepwise decomposition reaction take place during the course of calcination , described as: Bi 2 O 2 CO 3 → β-Bi 2 O 3 /Bi 2 O 2 CO 3 → β-Bi 2 O 3 → β-Bi 2 O 3 /α-Bi 2 O 3 → α-Bi 2 O 3 . The β-Bi 2 O 3 /Bi 2 O 2 CO 3 microspheres synthesized at 300 °C exhibit excellent photocatalytic activity under visible-light irradiation, which can degrade 99.8% OPP in 45 min. And the degradation rate of the heterostructured photocatalyst is approximately 2, 2.6, 6, 13, 80, and 827 times higher than that of single β-Bi 2 O 3 , mixed β-Bi 2 O 3 and Bi 2 O 2 CO 3 , commercial β-Bi 2 O 3 , α-Bi 2 O 3 , N-doped TiO 2 , and Bi 2 O 2 CO 3 , respectively. The superior photoreactivity of the β-Bi 2 O 3 /Bi 2 O 2 CO 3 is attributed to the enhanced charge separation and transfer due to the formation of p–n junction with large heterojunction interface, favorable band gap energy (2.27 eV), relatively high specific surface areas (12.5 m 2 g −1 ), and flower-like hierarchical micro/nano structures. In addition, the degradation intermediates including ethyl phenethyl ether, phenyl acetaldehyde, and phenylacetic acid are identified. And the results also reveal that the photogenerated holes and •O 2 − radicals are primarily reactive species in the photocatalytic system, which are the key factors responsible for the nearly complete mineralization of OPP.
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LWT-FOOD SCIENCE AND TECHNOLOGY
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2020
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IF 4.006
Sam Al-Dalali,Fuping Zheng,Baoguo Sun,Chenxi Zhou,Ming Li,Feng Chen
Effects of different brewing processes, such as the solarization process (i.e. with or without), selection of different types of rice as a raw material (i.e. normal or sticky rice), and addition of flavoring materials (i.e. presence or absence of spices or sugar), on the aroma profiles of three types of Chinese vinegars were investigated. These three vinegars were the Longmen aromatic sweet rice vinegar (LASRV), Longmen aromatic rice vinegar (LARV), and Longmen rice vinegar (LRV). Headspace solid-phase microextraction combined with gas chromatography-mass spectrometry (HS-SPME-GC-MS) and HS-SPME-aroma extract dilution analysis combined with gas chromatography-olfactometry (HS-SPME-AEDA-GC-O) were used to identify and quantify the volatile compounds, as well as the aroma-active compounds in the Longmen vinegars. Among the total of 68 volatile compounds that were identified, 49 were confirmed by their corresponding chemical standards. The results showed that the LASRV sample contained higher concentrations of acids, alcohols, esters, sulfides, acetal, and aromatic hydrocarbons because of effects of the solarization process and the presence of added sugar. The LARV sample was found to contain more aldehydes, pyrazines, ketones, phenols, lactone, and oxazoles, which might be contributed by the solarization process, as well as the addition of spices.
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JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2021
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IF 4.464
Shanhua Chen,Yanqing Huang,Ming Li,Panpan Sun,Xiaowei Lv,Bing Li,Liang Fang,Xiaohua Sun
Currently, development of OER/ORR bifunctional electric catalysts with low cost, high catalytic activity and stability is the key step to promote the practical application of zinc-air batteries. Herein, composite catalyst with manganese oxide on N and O co-doped carbon nanotubes encapsulated with FeCo alloy (CoFe@CNT/MnO) was prepared in situ on CFP by hydrothermal and CVD pyrolysis methods. Owing to rich C O functional group on in situ growing carbon nanotubes with 3D open structure, alloying effect of CoFe core metal embedded in carbon nanotube and the contribution of MnO supported on carbon nanotubes to ORR performance and stability, the CoFe@CNT/MnO catalyst exhibits low overpotential of 193 mV at the current density of 10 mA/cm 2 for OER, larger diffusion-limited current density of 5.75 mA/cm 2 and half-wave potential (E 1/2 = 0.85 V) for ORR, high specific capacity (810 mAh/g Zn ) and peak power density (164 mW/cm 2 ) with the Zn-air battery and superior stability. Moreover, the peak power density of the single CoFe@CNT/MnO-based quasi-solid state ZAB could reach 142 mW/cm 2 , and two quasi-solid state ZABs connected in series can power a mini fan.
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ACS Sustainable Chemistry & Engineering
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2025
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IF 7.3
Jun Zhang,Lanyu Luo,Jiankun Wang,Xinlan Xu,Tiantian Peng,Boyu Liu,Lingying Jiang,Meng Jin,Weimin Wu,Laizheng Luo,Huan Yi,Shi-Yu Lu
Optimizing the electronic configuration presents a promising approach to enhance intrinsic activity and minimize noble metal use in direct methanol fuel cells (DMFCs). Metal–support interactions, commonly employed to induce interfacial charge redistribution, play a crucial role in this optimization. This study successfully synthesized a Pd/O-OMS-2 electrocatalyst characterized by Pd–O–Mn interfacial chemical bonds and Mn3+/Mn4+ redox couples, serving as a stable bifunctional catalyst for both the oxygen reduction reaction (ORR) and methanol oxidation reaction (MOR). Unlike the mixed Pd–O/Mn bonds in Pd/O/Mn-OMS-2, the Pd–O–Mn bonds in Pd/O-OMS-2 not only enhance metal–support interactions and promote uniform dispersion of Pd nanoparticles but also facilitate electron transfer and the conversion between Mn3+ and Mn4+. These structural and compositional advantages endow the Pd/O-OMS-2 catalyst with superior ORR and MOR performance. The catalyst achieves a higher half-wave potential (E1/2) of 0.901 V, a mass activity of 0.937 A mgPd–1, and enhanced methanol permeability, outperforming Pd/O/Mn-OMS-2, Pd/OMS-2 and commercial Pd/C catalysts. Moreover, in an alkaline electrolyte, the Pd/O-OMS-2 catalyst follows a typical four-electron reaction pathway for ORR (O2 to H2O) and promotes faster reaction kinetics and charge transfer rates. In terms of durability, the Pd/O-OMS-2 catalyst exhibits minimal E1/2 decay (8 mV) and retains its structural integrity even after 10,000 cyclic voltammetry (CV) cycles. Additionally, it demonstrates the highest mass activity and stability during the MOR process. The exceptional ORR/MOR performance can be attributed to the synergistic effect of strong metal–support interactions, high Pd nanoparticle dispersion, and increased Mn3+ active species. The construction of interfacial chemical bonds offers a prospective strategy to optimize electronic configurations and develop advanced bifunctional electrocatalysts for ORR and MOR.
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高影响因子
FOOD CHEMISTRY
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2025
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IF 9.8
Shu Yang,Yijun Ren,Ganbing Zhang,Hui Xu
Single-atom nanozymes (SAzymes) exhibit superior catalytic activity, making it a promising candidate for constructing biosensors. Herein, we constructed a hollow carbon nanocages supported, N and O double-coordinated Fe SAzymes (HCNs@Fe SAs) through a template and pyrolysis strategy. Abundant oxygen atoms in pre-modified template SiO 2 @resorcinol-formaldehyde provided enough binding sites and promoted the dispersion and stabilization of metal atoms. Compared with non-O modified nanozymes, HCNs@Fe SAs exhibited an 8-fold enhancement in catalytic efficiency. Benefiting from its exceptional peroxide-like activity, a straightforward and dependable colorimetric sensing platform was developed to assess the activity of Acetylcholinesterase and its inhibitor organophosphorus pesticide (OP) in real water samples and fruit substrates, with the limit of detection of 0.02318 mU mL −1 and 0.85 ng mL −1 , respectively. An OPs test paper was also designed for point-of-care determination of OP with satisfactory sensitivity. This study provides a new strategy of template pre-modification to design SAzymes with enhanced catalytic efficiency.
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DOI
PubMed
高影响因子
Materials Research Express
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2021
·Vol. 8
·期 12
·125006
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IF 1.62
Peng She,Chun Yao,Jun Li,Hegang Bao,Xiuquan Xu,Hong Zhou
Co-doping is one of the most facile and efficient strategies for boosting the photocatalytic performance of g-C3N4. Herein, a series of Ti and O co-doped g-C3N4 (Ti/OCN) composites with excellent photocatalytic inactivation activity of Enterobacter cloacae (E. cloacae) were successfully synthesized by simple calcination of urea/formic acid/TiCl4 precursor. The obtained Ti/OCN-3 possessed the optimal photocatalytic capability with complete inactivation of 6.20 log CFU·ml−1 E. cloacae under 60 min of visible light irradiation. The improved photocatalytic disinfection performance was probably attributed to the synergistic effect of Ti and O co-doping, which led to an extended visible light response and accelerated charge transport efficiency. Furthermore, scavenger trapping and electron spin resonance (ESR) experiments revealed that ·O2−, h+ and ·OH were responsible for the enhanced inactivation activity. Overall, this research provided a promising strategy for fabricating g-C3N4-based catalysts to efficiently deal with the hazards of pathogenic microorganisms.
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高影响因子
CHEMICAL ENGINEERING JOURNAL
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2025
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IF 13.2
Qunfeng Chen,Daobin Tang,Qi Zhang,Zhiyuan Tang,Longlong Ma,Xinghua Zhang
Electron-metal support interactions (EMSI), which can tailor the microenvironment through electron transfer and coordination structure design, are an effective strategy to augment the intrinsic catalytic activity of catalysts. However, it remains challenging to establish the interfacial structure and interfacial electron configuration of catalysts to precisely regulate and enhance EMSI. In this work, the spatially defective sites and specific electronic states of Pd-UiO-66 were modified by in-situ constructing a W-O-Zr interface to strengthen the EMSI between Pd nanoparticles (Pd NPs) and UiO-66 support via implanting W into the defective Pd-UiO-66, realizing the effective hydrodeoxygenation (HDO) of vanillin (VAN) at room temperature (RT). The as-prepared catalyst Pd-UiO-66–0.24 W achieved > 99 % conversion of VAN and > 99 % yield of 2-methoxy-4-methylphenol (MMP) within 12 h, which surpassed the previous reports. The DFT analysis revealed that the narrow band-gap energy between Pd-UiO-66–0.24 W and H 2 molecule, along with the σ or π coordination adsorption between p and d orbitals of the implanted W and O in C=O, facilitated the activation of the H 2 molecules and reactant molecules. In addition, the constructed electron-deficient W-O-Zr interfacial sites provided more acidic sites that favor the cleavage of C-O bonds, promoting the HDO process of VAN. This work provides important insights into the modulation of EMSI, structural defects and acidic sites of metal–organic frameworks (MOFs)-based catalysts for efficient HDO of lignin-derived oxygenated compounds.
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高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2025
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IF 9.7
Siyuan Sun,Xiaoyun Zhang,Yang Sun,Fan Yang,Tianyu Bai,Cunhui Yu,Kexin Wei,Haojia Zhong,Qiang Wei,Yongfeng Li
Porous carbon coated manganese compounds nanoparticles (MnX, X = P, O or S) is a kind of potential catalysts for oxygen electrocatalysis due to the controllable d-band center, while the complex mechanism and performance difference is yet to reveal due to the lack of catalyst platform. Herein, the ORR catalytic activity and difference reason between manganese phosphide, oxide, and sulfide are tested and discussed via manganese compounds contained carbon-based catalyst platforms synthesis and Density functional theory (DFT). Manganese compounds nanoparticles active sites are uniformly distributed in porous carbon fibers/graphene composite, which guarantees the high mass transfer ability and conductive ability. Experiment results show that catalyst with Mn 2 P active sites exhibits best bifunctional catalytic activity for ORR/OER, which only need 0.89 V (vs. RHE) to achieve a half-wave potential (E 1/2 ) in ORR and 1.58 V (vs. RHE) to reach the overpotential at 10 mA cm −2 (E 10 ) in OER. Theoretical analysis shows that the d-band center of Mn in Mn 2 P is much closer to the Fermi level than MnS and MnO. The rate-determining process of Mn 2 P has the smallest energy barrier of 0.56 eV, indicating the best ORR activity. This work provides paves a solid way for the synthesis of carbon-based catalysts with manganese compounds active sites.
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PubMed
高影响因子
Oncotarget
·
2017
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IF 5.168
Ting Tao,Caimei He,Jun Deng,Yanjun Huang,Qiongli Su,Mei Peng,Meiling Yi,Kwame Oteng Darko,Hui Zou,Xiaoping Yang
Quercetin is a naturally existing compound and shows attractive anticancer properties for a variety of solid tumors including glioma, bladder cancer, hepatocellular carcinoma, breast cancer, hematological malignancies and prostate carcinoma. However, these anticancer properties have not been clinically approved due to unclear mechanistic information and its low bioactivity. In our previous study, we elucidated that quercetin activates AMPK pathway which is the major mechanism for its unique anticancer effect in bladder cancer. In the present study, we are trying to enhance its bioactivity by chemical modification using fluorination approach to prepare novel chemical entities, based on the principle of intermediate derivative method (IDM). The compound we obtained is named 8-trifluoromethyl-3,5,7,3′,4′-O-pentamethyl- quercetin (TFQ), characterized by NMR spectra and mass spectrum (MS). The results from MTT and cologenic assay in two human and one murine bladder cancer cell lines showed that TFQ exhibits more potent inhibition on the three bladder cancer cell lines than quercetin (Que) although this enhanced effects is not very dramatic. Furthermore, we found that the survival of normal bladder cells PEBC was not significantly suppressed by TFQ compared with Que. Western blot analysis showed that TFQ possess more potent AMPK activation than Que. The downstream of AMPK was further examined by western blot. TFQ treatment is able to inactivate mTOR signaling pathway with the regulation of mTOR, 4EBP1 and P70S6K. These results demonstrated that the fluorinated quercetin derivative TFQ inhibits bladder cancer cell growth through the AMPK/mTOR pathway. Altogether, our findings suggest that TFQ could serve as a new potential therapeutic agent for bladder cancer more effective than Que.
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PubMed
高影响因子
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