PeerJ
·
2019
·
IF 2.353
Hongli Yin,Ji Ma,Jichun Han,Maoru Li,Jing Shang
Background Quercetin (Qr), isoquercitrin (IQ), and quercetin-3-O-β-D-glucuronide (QG) are powerful phytochemicals that have been shown to exhibit disease prevention and health promotion properties. However, there may exist transformations between Qr, IQ, and QG in vivo . And the pharmacokinetic profiles of Qr, IQ, and QG have not been systematically compared. The pharmacokinetics study would be helpful to better understand the pharmacological actions of them. Methods Herein, we developed a reliable HPLC-MS method to compare the pharmacokinetics of Qr, IQ, and QG after separate (50 mg/kg) oral administration of them in rats, using puerarin as internal standard. The detection was performed using negative selected ion monitoring. This method was validated in terms of selectivity, linearity, precision, accuracy, extraction recovery, matrix effect, and stability; and shows reliabilities in monitoring the pharmacokinetic behaviors of these three compounds. Results Our results showed that after separate oral administration of Qr, IQ, and QG, all of the compounds could be detected in plasma. In addition, QG could be detected in the Qr group; Qr and QG could be measured in the IQ group; and Qr could be found in rat plasma after 1.5 h of QG administration. Moreover, the AUC 0− t of Qr in the; Qr group (2,590.5 ± 987.9 mg/L*min), IQ group (2,212.7 ± 914.1 mg/L*min), and QG group (3,505.7 ± 1,565.0 mg/L*min) was larger than the AUC 0− t of QG in the; Qr group (1,550.0 ± 454.2 mg/L*min), IQ group (669.3 ± 188.3 mg/L*min), and QG group (962.7 ± 602.3 mg/L*min). The AUC 0− t of IQ was the lowest among all groups. Discussion Quercetin, IQ, and QG can all be absorbed into plasma. A mutual transformation exists between Qr and QG, and IQ can be metabolized into Qr and QG in SD rats. These results would provide a meaningful basis for understanding the pharmacological actions of these three compounds.
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高影响因子
RSC Advances
·
2021
·Vol. 11
·期 61
·38915-38924
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IF 3.361
Peng Li,Xin Wang,Guifang Wang,Li Zhao,Yuwen Hong,Xianzhi Hu,Futing Zi,Huiling Cheng
Using Cr(VI) as the imprinted ions and 2-allyl-1,3-diphenyl-1,3-propanedione (ADPD) (a compound synthesized by independent design) as the functional monomer, a series of chromium ion-imprinted composite membranes (Cr(VI)-IICMs) and corresponding non-imprinted composite membranes (NICMs) were synthesized and tested. The results showed that the Cr(VI)-IICM10 membrane prepared under optimal experimental conditions exhibited a high adsorption capacity towards Cr(VI) (Q = 30.35 mg g−1) and a high imprinting factor (α = 2.70). The structural characteristics of Cr(VI)-IICM10 and NICM10 were investigated using FE-SEM, ATR-FTIR, and BET techniques combined with UV-Vis photometry and inductively coupled plasma emission spectrometry (ICP-OES) to evaluate the adsorption performance and permeation selectivity, while the effect on adsorption permeance of varying the experimental conditions including the solvent type, pH, and temperature was also investigated. The results showed that Cr(VI)-IICM10 is a mesoporous material with excellent permeation selectivity, reusability, and favorable pH response, and that its adsorption behavior is in accordance with the Langmuir model and pseudo-first-order kinetics. Thus, Cr(VI)-IICM10 shows great potential towards utilization as a “smart membrane” to control the separation and removal of Cr(VI) in wastewater, and also proved a reasonable design of the new functional monomer ADPD.
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高影响因子
Journal of Materials Chemistry B
·
2016
·Vol. 4
·期 22
·3903-3910
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IF 4.872
Genxing Zhu,Ruibo Zhao,Yaling Li,Ruikang Tang
Multifunctional Gd,Ce,Tb co-doped β-tricalcium phosphate (TCP) porous nanospheres are prepared by a facile solvothermal strategy with trimethyl phosphate as the phosphorus source. The as-prepared nanomaterial (average diameter of 100 nm) has a multiple level pore size distribution with the specific surface area of 124.33 m2 g−1, which benefits drug loading. Its photoluminescent and magnetic multifunctions are realized by the co-doping of Gd3+, Ce3+ and Tb3+ ions, which make the nanomaterial promising for both fluorescence and magnetic resonance imaging techniques. Furthermore, the nanomaterial exhibits excellent cytocompatibility and a relatively high doxorubicin loading capacity as well as sustained pH-sensitive drug release behaviour. It is suggested that the Gd,Ce,Tb co-doped β-TCP porous nanospheres are promising for applications in the biomedical fields such as multifunctional drug delivery systems and tissue engineering scaffolds with bioimaging guidance.
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高影响因子
Arabian Journal of Chemistry
·
2017
·
IF 4.553
Parbeen Singh,Xiaohong Ren,Yaping He,Li Wu,Caifen Wang,Haiyan Li,Vikramjeet Singh,Jiwen Zhang
The fabrication of supramolecular host in combination biomolecules is an interesting idea in modern drug delivery for development of new polymer with advanced chemical and biological properties. Herein, hyper-crosslinked copolymer was fabricated from β-cyclodextrin (β-CD) and sialic acid (SA) monomers , which can be undoublty considered as a new class of copolymer. The as-synthesized copolymer has complexation properties, which could cover the drug within the structure and deliver to the site of action. The well-known inclusion capability of β-CD and targeted efficacy of SA made it more appropriate for targeted drug delivery. The copolymer was characterized using a wide range of spectroscopic and microscopic techniques such as synchrotron radiation based FTIR spectroscopy (SR-FTIR), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), and powder X-ray diffraction (PXRD). The surface area and porosity were calculated by using Nitrogen adsorption method. Doxorubicin (Dox) was selected as a model drug to evaluate the loading efficiency and cellular penetration ability of the copolymer. The copolymer showed high adsorption towards Dox with no significant cytotoxic effects on HeLa cells as proved by cell viability assay. High cellular penetration of Dox loaded copolymer was also recorded by confocal microscopy when compared with free Dox in HeLa cells at 4 h of exposure. Thus, β-CD-SA copolymer could be a useful carrier for targeted drug delivery of cancer and has the potential for further investigation in viral and nervous disease due to the targeting ability of SA.
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高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2018
·
IF 3.141
Junhui Rong,Juan Han,Yang Zhou,Lei Wang,Chunmei Li,Yun Wang
In enzymatic conversion of biomass, how to degrade cellulose into fermentable glucose in an economic, efficient, and clean way has become an important subject. As for the application of cellulase in cellulose degradation, the process optimization in enzyme engineering is urgently desired. The traditional multistep purification processes lead to rising production costs and reduced activity of cellulase; meanwhile, the difficulty in reusability of cellulase has also become a big baffle in the cost-effective application of cellulase in biomass degradation. In this paper, the biocatalyst Glu-linker-ELP-GB (GLEGB) containing binary tags, elastin-like polypeptide (ELP), and graphene-binding (GB), was constructed to simplify the purification and immobilization of β-glucosidase (Glu) from Coptotermes formosanus. A high recovery rate (97.2%) and purification fold (18.7) of GLEGB was obtained by only one round of inverse transition cycling (ITC) with 0.5 M (NH4)2SO4 at 25 °C in a short incubating time of 10 min. The purification performance of the one-round ITC method is superior to the commonly used Ni-NTA resin affinity method. Furthermore, the high loading amounts of GLEGB immobilized on GO (698.2 mg g–1) and C3N4 (527.3 mg g–1) were achieved by the synergistic effects of ELP and GB tags. The storage stability and thermal stability of GLEGB was significantly enhanced after immobilization. The recombinant GLEGB immobilized on GO, MGO, graphite, C3N4, C200, and C400 retained 71.4%, 69.5%, 75.1%, 61.2%, 73.5%, and 80.2% of their initial activities respectively after eight cycles. It is worth mentioning that the Km values of GLEGB immobilized on lamellar carbon materials including GO, MGO, and C3N4 are very close to free GLEGB, showing a high affinity of recombinant GLEGB to substrate. To our knowledge, this is the first report on enzyme-linker-ELP-GB system with wide application prospect in the efficient purification and immobilization of enzyme, which can achieve the goal of reducing cost and improving efficiency of biocatalyst in enzymatic conversion of biomass.
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PROCESS BIOCHEMISTRY
·
2022
·
IF 4.885
Jinmao Ma,Dongrun Wang,Wei Zhang,Xiangyu Wang,Xia Ma,Minsheng Liu,Quanlin Zhao,Lincheng Zhou,Sanxiang Sun,Zhengfang Ye
The host–guest β-cyclodextrin has attracted much attention for potential application in various fields due to its low price, natural pores, and functionality. Herein, we report a method using 1,4-phenylene diisocyanate (PPDI) to fabricate β-cyclodextrin (CD)-modified poly(chloromethyl styrene) (PCMS) resin, namely, PCMS@PPDI-CD. β-Cyclodextrin was utilized to improve the adsorption performance of PCMS, which was then applied to anchor β-cyclodextrin powder. The product exhibits excellent renewability and availability due to the application of PCMS. The adsorption performance was fully exerted by the combination of β-cyclodextrin and PCMS. SEM mapping, FTIR, TG , BET, and XPS analyses were conducted to explore the morphological and structural properties of PCMS@PPDI-CD. Adsorption experiments show that PCMS@PPDI-CD removed 100 % of TC and 75 % of Cu(Ⅱ), and the maximum adsorption capacity for Cu(Ⅱ) and TC reached 187.6 and 266.4 mg g −1 , respectively. After recycling six times, the adsorption ability of Cu(Ⅱ) and TC still achieved excellent removal performance. In addition, the chelation of the lone N pair, the hydrophobicity of the cavity, H-bonding, complexation, n-π interaction, and porous structure showed the possible mechanism. We used computational formulas to simulate the adsorption state and found that the resin is theoretically feasible. Most importantly, we propose a novel application of PCMS@PPDI-CD. This work shows that the resulting PCMS@PPDI-CD is highly efficient and can be easily recycled, making it an attractive resin for adsorption.
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
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IF 5.401
Xiyue Xie,Haijun Wang,Li Zhang,Yuting Liu,Yaqin Chai,Yali Yuan,Ruo Yuan
The shortened distance between luminophor and coreactant can effectively enhance luminous efficiency in electrochemiluminescence (ECL) system owing to the improvement of electron transfer rate. Here, a novel sandwich-type ECL immunosensor based on N-(aminobutyl)-N-(ethylisoluminol) (ABEI) as luminophor was constructed by integrating hollow gold nanoparticles (HAuNPs), l -cysteine ( l -Cys) and ferrocene (Fc) in a complex for multiple signal amplification. The HAuNPs with large specific surface areas and superior conductivity were first used as scaffolds to load abundant coreactant l -Cys, aiming to further assemble catalyzer Fc stably. Through host-guest recognition, the β-cyclodextrin (β-CD) functionalized ABEI thus subsequently decorated on Fc, bringing in shortened distance between luminophor ABEI and coreactant l -Cys with high ECL signal. Moreover, the catalyzer Fc and HAuNPs could dramatically promote decomposition of another coreactant H 2 O 2 to produce abundant superoxide radical (O 2 ˉ ) and hydroxyl radical (OH ) for further signal amplification. Taking β2-microglobulin (β2 M) as model analyte, the developed immunosensor exhibited a superior performance for detection β2 M in the range of 1.0 × 10 −3 –1.0 × 10 3 ng mL −1 with a detection limit of 2.6 × 10 −1 pg mL −1 . The developed strategy realized solid-state ECL detection and multiple ECL signal amplification, providing a novel sensitive detection method for β2 M, which may show a great promise for the clinical application.
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JOURNAL OF CELLULAR BIOCHEMISTRY
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2019
·Vol. 120
·期 10
·17273-17282
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IF 3.448
Min-yi Lv,Chuan-jian Shi,Fei-fei Pan,Jiang Shao,Lu Feng,Guoqin Chen,Caiwen Ou,Jin-fang Zhang,Wei-ming Fu
Consumption of dietary ellagitannins (ETs) has been proven to benefit multiple chronic health disorders including cancers and cardiovascular diseases. Urolithins, gut microbiota metabolites derived from ETs, are considered as the molecules responsible for these health effects. Previous studies have demonstrated that urolithins exhibit antiproliferative effects on prostate, breast, and colon cancers. However, as for hepatocellular carcinoma (HCC), it remains elusive. Herein, we aim to investigate the function of urolithin B (UB), a member of urolithins family, in HCC. The effects of UB on cell viability, cell cycle and apoptosis were evaluated in HCC cells, and we found UB could inhibit the proliferation of HCC cells, which resulted from cell cycle arrest and apoptosis. Furthermore, UB could increase phosphorylated β-catenin expression and block its translocation from nuclear to cytoplasm, thus inducing the inactivation of Wnt/β-catenin signaling. Using a xenograft mice model, UB was found to suppress tumor growth in vivo. In conclusion, our data demonstrated that UB could inhibit the proliferation of HCC cells in vitro and in vivo via inactivating Wnt/β-catenin signaling, suggesting UB could be a promising candidate in the development of anticancer drugs targeting HCC.
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RSC Advances
·
2020
·Vol. 10
·期 47
·28397-28407
·
IF 3.119
Long Qin,Jiang Yi,Lai Xuefei,Liao Li,Xie Kenan,Xie Lu
Porous β-tricalcium phosphate (β-Ca3(PO4)2, β-TCP) scaffolds are widely applied in the field of bone tissue engineering due to their nontoxicity, degradability, biocompatibility, and osteoinductivity. However, poor compressive strength and a lack of antibacterial properties have hindered their clinical application. In order to address these disadvantages, graphene (G) and silver nanoparticles were introduced into β-TCP through a two-step method. In the synthesis process, G-β-TCP was prepared via an in situ synthesis method, and then silver nanoparticles and HAp particles were coated on the surface of the G-β-TCP scaffold in an orderly fashion using dopamine as a binder. From the results of characterization, when the content of graphene was 1 wt% of β-TCP, the G-β-TCP scaffold had the highest compression strength (127.25 MPa). And core–shell G-β-TCP-Ag-HAp not only had reduced cytotoxicity via the continuous release of Ag+, but it also achieved long-term antibacterial properties. Besides, the material still showed good cell activity and proliferation.
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高影响因子
JOURNAL OF POWER SOURCES
·
2019
·
IF 7.467
Qiyao Guo,Jihuai Wu,Yuqian Yang,Xuping Liu,Jinbiao Jia,Jia Dong,Zhang Lan,Jianming Lin,Miaoliang Huang,Yuelin Wei,Yunfang Huang
The introduction of upconversion materials as spectral converter into photovoltaic devices to harvest and convert near infrared photons to visible photons that can thus be absorbed by perovskite layer is a promising strategy for enhancing the performance of perovskite solar cells . Hence, β-NaYF 4 :Yb 3+ /Er 3+ /Sc 3+ @NaYF 4 rare-earth doped upconversion nanoparticles with core-shell structure are synthesized. The doped rare-earth ions Yb 3+ and Er 3+ convert near infrared light to red and green light, and the Sc 3+ ions enhance the upconversion luminescence efficiency, which broaden the absorption range of solar irradiation and generate extra photocurrent in the solar cells. The surface defects of β-NaYF 4 :Yb 3+ /Er 3+ /Sc 3+ is passivated by the even NaYF 4 shell, which results in the obvious enhancement in upconversion emission intensity. Consequently, the perovskite solar cell based on the rare-earth doped upconversion nanoparticle mesoporous layer achieves a high power conversion efficiency of 20.19%, while the device based on TiO 2 mesoporous layer gets an efficiency of 17.44%.
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FOOD HYDROCOLLOIDS
·
2020
·
IF 7.053
Hao Yin,Chenxiao Wang,Jin Yue,Yun Deng,Shunshan Jiao,Yanyun Zhao,Jie Zhou,Ting Cao
Essential oils can be capsulated by cyclodextrin to enhance its thermal stability and preserve the bioactivity. In this study, 1,8-cineole was used as a guest molecule to prepare the cyclodextrin inclusion complexes (ICs). The formulation and processing parameters were optimized using the response surface methodology (RSM), and the optimal conditions were identified to be: 2.30 h of encapsulating time, 30.1 °C of encapsulating temperature and 0.99 of molar ratio of 1,8-cineole to HP-β-CD. The corresponding encapsulation efficiency and loading efficiency were 83.17% and 8.40%, respectively. The produced 1,8-cineole/HP-β-CD ICs was characterized by SEM, FTIR and TGA, and the results proved the enhanced thermal stability of IC. The release behavior and kinetics of IC was investigated and showed that at elevated temperature and relative humidity, the releasing rate of 1,8-cineole from the ICs increased and followed the Fick's law.
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INTERNATIONAL DAIRY JOURNAL
·
2022
·
IF 3.572
Yaqi Tu,Lei Guan,Fazheng Ren,Hao Zhang
This study sought to research the antigenicity and functional characteristics of Maillard reaction products (MRPs) arising from the human breast milk oligosaccharide , 2′-fucosyllactose (2′-FL), and β-lactoglobulin (β-LG). The MRPs were prepared at 60 °C, 96 h and 79% relative humidity. During the Maillard reaction, the content of ultraviolet-absorbing and fluorescence compounds, and browning products increased. The free amino groups content decreased by 61.5% during the first 48 h then did not change significantly. The results of sodium dodecylsulphate polyacrylamide gel electrophoresis showed that high molecular mass MRPs were generated and the intrinsic fluorescence spectra suggested a masking effect of 2′-FL. The surface hydrophobicity of β-LG-2′-FL increased and the antigenicity of the MRPs reduced significantly compared with the control β-LG, with the inhibition rate reaching 49.09%. The 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and ferric reducing/antioxidant power assay revealed significant increases in the antioxidant activity of the MRPs.
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危险品化学品经营许可证（带存储）