PeerJ
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2024
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IF 2.3
Zhaohong Wang,Hui Chen,Xufan Cai,Heqi Bu,Shengzhang Lin
Background Andrographolide (Andro), an extract of Andrographis paniculate (Burm.f.) Wall. ex Nees (Acanthaceae), possesses diverse biologically active properties. However, the precise mechanisms and effects of Andro on pancreatic cancer (PC) remain unclear. Methods The cytotoxic potential of Andro and underlying mechanism towards PC cells was investigated through in vitro experiments and a xenograft mouse model. PC cells were first subjected to varying concentrations of Andro. The reactive oxygen species (ROS) was assessed using flow cytometry and DCFH-DA staining. The apoptosis rate was detected by flow cytometry. Additionally, western blot was applied to evaluate the expression levels of cleaved-caspase-3, DJ-1, LC3-I, LC3-II, and p62. To further elucidate the involvement of ROS accumulation and autophagy, we employed N-acetylcysteine as a scavenger of ROS and 3-Methyladenine as an inhibitor of autophagy. Results Andro demonstrated potent anti-proliferative effects on PC cells and induced apoptosis, both in vitro and in vivo . The cytotoxicity of Andro on PC cells was counteracted by DJ-1 overexpression. The reduction in DJ-1 expression caused by Andro led to ROS accumulation, subsequently inhibiting the growth of PC cells. Furthermore, Andro stimulated cytoprotective autophagy, thus weakening the antitumor effect. Pharmacological blockade of autophagy further enhanced the antitumor efficacy of Andro. Conclusion Our study indicated that ROS accumulation induced by the DJ-1 reduction played a key role in Andro-mediated PC cell inhibition. Furthermore, the protective autophagy induced by the Andro in PC cells is a mechanism that needs to be addressed in future studies.
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高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2024
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IF 4.9
Tingting Li,Yukun An,Jiashuang Li,Zhiming Zhang,Lin Liu,Libao An
A bimetallic Ln-MOF nanofibrous membrane (NFM) was successfully fabricated by in situ growth of Eu/Tb-MOF crystals onto electrospun polycaprolactam (PA6) nanofibers . For better combining with PA6 nanofibers , the Eu/Tb-MOFs were made into nanocrystals . Characterization with PXRD , FT-IR, EDS and XPS confirmed that the crystal structure of the Eu/Tb-MOFs maintained well on PA6 NFM. The as-prepared Eu/Tb-MOF@PA6 NFM can be used as a colorimetric fluorescent test strip for instant detection of the volume ratio of 1, 4-dioxane in 1, 4-dioxane-glycol mixture. With the increase of 1, 4-dioxane, the test strip presented color changes from green to red, which can be easily distinguished by the naked eye. In view of this, the conversion rate of the condensation reaction of glycol to 1, 4-dioxane will be preliminarily estimated without the help of equipment. More importantly, the test strip exhibited better convenience and reversibility compared with powdered Ln-MOFs because no separation process was needed for test strip before and after recovering. Benefiting from sufficient amide groups in PA6 for anchoring Eu/Tb-MOF particles, the test strip showed satisfactory stability in cycling experiments. This work will provide a new insight into the development of fluorescent test strip based on electrospun MOF NFM for quantitatively monitoring the reaction process.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2025
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IF 9
Wei Chen,Pengfei Wang,Weike Jia,Huidan Zeng
Silicalite-1 zeolites/polymer composite membranes are among the most reliable materials for the pervaporation separation of m-X from xylene isomers. However, the separation factors of the present silicalite-1 zeolites/polymer composite membrane are low. This work has succeeded in fabricating dense and defect-free silicalite-1/PVA composite membrane for first improving the pervaporation separation performance of m-X/p-X isomers. The silicalite-1/PVA composite membrane was prepared by combining solution scraping and vacuum thermal crosslinking methods with fumaric acid as a crosslinker (denoted as silicalite-1/PVA-V). The silicalite-1/PVA-V composite membrane with a thickness of 15.3 μm, prepared with a fumaric acid/monomeric unit of PVA molar ratio of 0.05 after vacuum thermal crosslinking at 160 ℃ for 2 h, exhibited dense and defect-free structure with extremely low xylene swelling degree, good structural stability and mechanical properties as well as excellent pervaporation separation performance. Compared to the pure PVA membrane, the silicalite-1/PVA-V composite membrane exhibited better selective separation performance of m-X isomer. The temperature, amount of fumaric acid, crosslinking degree and membrane thickness significantly influenced the pervaporation separation performance. The optimum selective separation factor of m-X/p-X isomers and the corresponding total permeate flux were about 9.85 and 59.15 g·m −2 ·h −1 (60 ℃), respectively. The pervaporation experiments of long-time and repetitively prepared membrane demonstrated that the silicalite-1/PVA-V composite membrane had long-term structural stability and reproducibility. To demonstrate scalability, we showed that our strategy could produce large-size silicalite-1/PVA-V composite membrane with an area of 1000 cm 2 , which exhibited m-X separation performance similar to that of small membrane coupons. The present work provided a potentially scalable method for producing high-quality silicalite-1/PVA composite membrane for enhancing the pervaporation separation performance of m-X from xylene isomers.
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ELECTROCHEMISTRY COMMUNICATIONS
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2012
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IF 4.859
Bin Li,Mengqing Xu,Tiantian Li,Weishan Li,Shejun Hu
In this paper, we reported a novel solid electrolyte interphase (SEI) formation additive, prop-1-ene-1,3-sultone (PES), used in propylene carbonate (PC)-based electrolyte for lithium ion batteries . The effect of PES on the co-intercalation of PC into anode and the battery performance was investigated by energy calculation, cyclic voltammetry and battery charge–discharge test. The theoretical calculations and the voltammograms show that PES can be reduced prior to propane sultone (PS), a practical SEI formation additive for lithium ion batteries. Charge–discharge test shows that the use of PES can suppress successfully the co-intercalation of PC with lithium ions into graphite and the LiCoO 2 /graphite battery using PES exhibits better performance than that using PS as the SEI formation additive.
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高影响因子
RSC Advances
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2015
·Vol. 5
·期 76
·62241-62247
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IF 3.84
Yibo He,Chao Wan,Qinghua Zhang,Xiaoli Zhan,Dang-guo Cheng,Fengqiu Chen
Friedel–Crafts alkylation of benzene with 1-dodecene, which is an important reaction of synthetic detergent, was studied using the catalyst [bmim][TFSI]/AlCl3 (1-butyl-3-methylimidazolium bis((trifluoromethyl)sulfonyl)imide/AlCl3). These ionic liquid catalysts show biphasic behaviors at a specific condition. Active site species and Lewis acidity were determined by NMR and acetonitrile probe FT-IR. NMR spectroscopy investigations indicated that “coordinated” and “uncoordinated” [TFSI]− existed in the ionic liquid catalyst system simultaneously, while tetrahedral 4-coordinate Al2Cl7− was the main active species for Friedel–Crafts alkylation. The complex [AlClx(TFSI)y]− tends to decompose and release 4-coordinated Al2Cl7− and [TFSI]−. The phase behavior study of the ionic liquid [bmim][TFSI]/AlCl3 demonstrated that the upper phase could be used as an efficient heterogeneous catalyst when AlCl3/IL ≥ 1.5. Due to the presence of the anion [TFSI]− around the active sites, especially [TFSI]− at the “uncoordinated” state, the ionic liquid formed a stable and hydrophobic chemical environment, which enhanced catalyst durability. The influence of various reaction conditions including catalyst reusability on the alkylation reaction was studied, and the potential reasons for catalyst deactivation were discussed. The highest 2-LAB selectivity was more than 50% when 1-dodecene conversion was nearly 100%. Compared with liquid or solid acid catalyst, ionic liquid catalysts have many advantages concerning energy and the environment.
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高影响因子
CHEMICAL ENGINEERING JOURNAL
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2017
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IF 6.216
Guoqiang Wu,Zhengguo Lin,Liang Li,Lei Zhang,Yanping Hong,Wenjun Wang,Chao Chen,Yan Jiang,Xianzai Yan
An efficacious approach to improve the catalytic performance of titanium silicalite-1 (TS-1) catalysts in epoxidation of allyl chloride with hydrogen peroxide has been developed. A series of modified TS-1 catalysts were prepared by corroding the classic TS-1 catalyst with different concentrations of tetrapropyl ammonium hydroxide (TPAOH) under controlled conditions. The resultant catalyst samples were characterized by XRD, ICP, N 2 -adsorption, FT-IR, UV–vis, SEM and TEM. The catalytic activity of the modified TS-1 catalysts is significantly improved, which could be attributed to the increase of the mesopore volume and pore diameter and decrease of the diffusion limitation but without obvious changes of the framework structure, framework Ti content, the outer surface shape and crystal size of the modified TS-1. Furthermore, the kinetics of the epoxidation of allyl chloride over TS-1-0.05 catalyst was also systematically studied, the apparent orders of reaction with respect to H 2 O 2 and allyl chloride are 0.68 and 0.62, respectively, and the activation energy of the epoxidation reaction on the TS-1-0.05 catalyst is 57.4 kJ mol −1 .
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JOURNAL OF CHEMICAL THERMODYNAMICS
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2016
·
IF 2.196
Hujun Xie,Lijiang Zhao,Chengcheng Liu,Yifan Cao,Xiaoxing Lu,Qunfang Lei,Wenjun Fang
The densities of aqueous solutions of glycine, l -serine, l -alanine, l -proline with the ionic liquid (IL), 1-phenylpiperazinium tetrafluoroborate ([Phpi][BF 4 ]) at the IL concentrations of (0.025, 0.055 and 0.100) mol·kg −1 have been measured at the temperatures of (298.15, 303.15 and 308.15) K. On the basis of the experimental results, the apparent molar volume ( V Ф ), standard partial molar volume ( V Ф 0 ), transfer partial molar volume (Δ tr V Φ 0 ) and hydration number ( n H ) have been calculated. The hydrophilic–hydrophilic, hydrophobic–hydrophilic and hydrophobic–hydrophobic interactions are involved in the studied systems of {[Phpi][BF 4 ] + amino acids + H 2 O. These volumetric parameters can help to understand the mixing effects and other complex biological processes between amino acids and ionic liquid aqueous solution.
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高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
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2017
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IF 2.323
Huanhuan Sun,Cheng Liu,Zhongfeng Geng,Yang Lu,Yixuan Chen
In this article, isobaric vapor–liquid equilibrium (VLE) data for three binary systems of acetaldehyde + ethanol, acetaldehyde + ethyl acetate, and acetaldehyde +1-butanol were determined using a modified Rose–Williams equilibrium still at 101.3 kPa. The experimental data were confirmed by the area method of Herington and point-to-point method of Van Ness. Activity coefficient models, Wilson, NRTL, and UNIQUAC were employed to correlate the experimental VLE values. Both models showed a good agreement and the binary interaction parameters were estimated.
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高影响因子
JOURNAL OF CHEMICAL THERMODYNAMICS
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2016
·
IF 2.196
Yaran Yin,Chunying Zhu,Youguang Ma
Densities and viscosities of binary {[Bmim][BF 4 ] + H 2 O}, {[Bmim][BF 4 ] + MEA}, (MEA + H 2 O) and ternary mixtures {[Bmim][BF 4 ] + MEA + H 2 O} were measured at T = (293.15–333.15) K. The volumetric and viscometric properties, such as excess molar volume V E , viscosity deviation Δ η , and excess Gibbs energy of activation of viscous flow Δ G ∗E for all mixtures, and apparent molar volume, excess partial molar volume and Grunberg-Nissan interaction parameter G 12 for binary mixtures, were deduced from experimental results, and the intermolecular interactions in solutions were also analysed. The excess molar volumes were correlated using the Redlich-Kister polynomial equation for binary mixtures, and Singh et al. equation for the ternary mixture with corresponding binary parameters. The viscosities of binary and ternary solutions were respectively fitted by Jouyban-Acree equation and its extended equation at each measurement temperature, the correlated values are in good agreement with the corresponding experimental data.
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高影响因子
CHINESE JOURNAL OF CATALYSIS
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2016
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IF 2.628
Dun Deng,Yun Zhang,Aijun Sun,Yunfeng Hu
We previously functionally characterized a novel marine microbial GDSL lipase MT6 and identified that the stereo-selectivity of MT6 was opposite to that of other common lipases in trans-esterification reactions. Herein, we have investigated the use of MT6 in stereo-selective biocatalysis through direct hydrolysis reactions. Notably, the stereo-selectivity of MT6 was also demonstrated to be opposite to that of other common lipases in hydrolysis reactions. Parameters, including temperature, organic co-solvents, pH, ionic strength, catalyst loading, substrate concentration, and reaction time, affecting the enzymatic resolution of racemic 1-phenylethyl acetate were further investigated, with the e.e. of the final ( S )-1-Phenylethanol product and the conversion being 97% and 28.5%, respectively, after process optimization. The lengths of side chains of 1-phenylethyl esters greatly affected the stereo-selectivity and conversion during kinetic resolutions. MT6 is a novel marine microbial GDSL lipase exhibiting opposite stereo-selectivities than other common lipases in both trans-esterification reactions and hydrolysis reactions.
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Cell Death & Disease
·
2017
·Vol. 8
·期 8
·e2988
·
IF 5.965
Yan Siyuan,Liu Ling,Ren Fengxia,Gao Quan,Xu Shanshan,Hou Bolin,Wang Yange,Jiang Xuejun,Che Yongsheng
Deficiency of autophagy has been linked to increase in nuclear instability, but the role of autophagy in regulating the formation and elimination of micronuclei, a diagnostic marker for genomic instability, is limited in mammalian cells. Utilizing immunostaining and subcellular fractionation, we found that either LC3-II or the phosphorylated Ulk1 localized in nuclei, and immunoprecipitation results showed that both LC3 and Unc-51-like kinase 1 (Ulk1) interacted with γ -H2AX, a marker for the DNA double-strand breaks (DSB). Sunitinib, a multi-targeted receptor tyrosine kinase inhibitor, was found to enhance the autophagic flux concurring with increase in the frequency of micronuclei accrued upon inhibition of autophagy, and similar results were also obtained in the rasfonin-treated cells. Moreover, the punctate LC3 staining colocalized with micronuclei. Unexpectedly, deprivation of SQSTM1/p62 alone accumulated micronuclei, which was not further increased upon challenge with ST. Rad51 is a protein central to repairing DSB by homologous recombination and treatment with ST or rasfonin decreased its expression. In several cell lines, p62 appeared in the immunoprecipites of Rad51, whereas LC3, Ulk1 and p62 interacted with PARP-1, another protein involved in DNA repair and genomic stability. In addition, knockdown of either Rad51 or PARP-1 completely inhibited the ST-induced autophagic flux. Taken together, the data presented here demonstrated that both LC3-II and the phosphorylated Ulk1 localized in nuclei and interacted with the proteins essential for nuclear stability, thereby revealing a more intimate relationship between autophagy and genomic stability.
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PubMed
高影响因子
TALANTA
·
2016
·
IF 4.035
Wenchang Wang,Juntao Wang,Shuaihua Zhang,Penglei Cui,Chun Wang,Zhi Wang
A novel covalent organic framework, Schiff base network-1 (SNW-1), was synthesized and used as a solid-phase microextraction (SPME) fiber coating material . The SNW-1 coated SPME fiber was fabricated by a covalent chemical cross-linking between the SNW-1 nanocomposite and a silanol-functionalized stainless steel wire substrate. Scanning electron microscopy and nitrogen isothermal adsorption results indicate that the new fiber coating exhibited a porous, homogenous surface with the Brunauer-Emmett-Teller surface of 668 m 2 g −1 . The prepared fiber was explored for the SPME of phenols from honey samples prior to their determination by gas chromatography-mass spectrometry. The developed method had large enrichment factors (136–816), low limits of detection (0.06–0.2 ng g −1 ), good linearity (0.1–100.0 ng g −1 ) and repeatability (<9.7%) for phenols. The recoveries for spiked phenols (1.0 ng g −1 and 10.0 ng g −1 ) in Wolfberry , Robinia and Codonopsis honey samples were in the range of 84.2–107.2% with the relative standard deviations ranging from 3.8% to 12.7%. The developed method was suitable for the determination of phenols from honey samples.
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