JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
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2022
·Vol. 59
·期 12
·4653-4662
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IF 3.117
Chen Hongshuo,Liu Haibin,Cui Chuanjin,Zhang Wensi,Zuo Yueming
In this study, a simple label-free biosensor for Brucella was constructed, which based on the screen-printed carbon electrode (SPCE) modified by Recombinant protein G/gold nanoparticles/graphene oxide (RpG/Au/GO). The impedance responses of the proposed biosensor were measured by electrochemical AC impedance method in Brucella antigen gradient concentration solutions. The results showed that the linear range of this biosensor was from 1.6 × 10 2 CFU/mL to 1.6 × 10 8 CFU/mL with the minimum detection limit of 3.2 × 10 2 CFU/mL (S/N = 3). Moreover, the biosensor for Brucella detection possessed acceptable reproducibility with a relative standard deviation of 5.15% and acceptable stability with a relative standard deviation of 4.68%. The spiked recovery rate in actual pasteurized milk samples was more than 92% . Therefore, the developed biosensor exhibits excellent prospects in the selective quantification detection of Brucella abortus .
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COLLOIDS AND SURFACES B-BIOINTERFACES
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2022
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IF 5.999
Lanxin Xu,Yongling Zhang,Jun Zhou,Tao Jiang
The effective monitoring of nucleotides and their metabolites is critical for the prevention of various genetic metabolic diseases. In this aspect, surface-enhanced Raman scattering (SERS) as an ultrasensitive and nondestructive sensing techniques has spurted an attractive prospect. Henceforth, an effective SERS-based analysis of adenosine monophosphate (AMP) as well as adenine was realized here by utilizing an innovative kind of self-cleaning substrate constructed with graphitic carbon nitride and Au nanoflowers (g-C 3 N 4 @Au NFs). Taking advantages of the numerous nanotip-triggered “hotspot” regions of Au NFs as well as the excellent photocatalytic degradation capability of g-C 3 N 4 matrix, the hybrid substrate not only brought about a low limit of detection (LOD) of 5.01 × 10 −10 M, but also yielded an attractive self-cleaning property. Particularly, these fascinating features of the proposed g-C 3 N 4 @Au NFs facilitated a recyclable monitoring of AMP and adenine in serum with reliable sensitivity and stability. The achieved wide linear range from 10 −4 to 1 mg/mL together with the LOD values down to 10 −5 mg/mL all envisioned that it is possible to monitor and investigate human metabolic processes by SERS protocol.
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CHEMICAL ENGINEERING JOURNAL
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2022
·
IF 16.744
Xuemei Zeng,Yihang Ruan,Qi Chen,Shuangqian Yan,Wei Huang
Designing nanozyme hybrids with cascade catalytic activities and coupling effects has significant promises in tumor therapy through concerted effects. In this work, a goethite-derived porous Fe 2 O 3 /Au hybrid nanozyme was fabricated for synergistic treatment of triple negative breast cancer. The as-prepared porous Fe 2 O 3 nanoparticle exhibits excellent peroxidase-like activity compared to goethite particles, which can generate hydroxyl radicals in the tumor microenvironment. Furthermore, the Fe 2 O 3 nanoparticle induces ferroptosis through intracellular lipoperoxide accumulation. After decorating glucose oxidase-mimicking gold nanoparticles, the Fe 2 O 3 /Au hybrid nanozyme is able to catalyze glucose into gluconic acid and H 2 O 2 . The glucose consumption deprives the energy of the tumor cell, and the generated H 2 O 2 can be converted to hydroxyl radicals through the biocatalytic reaction. Importantly, the coupling of gold nanoparticles enhances the photothermal conversion of the porous Fe 2 O 3 nanoparticle. Thus, the designed Fe 2 O 3 /Au hybrid nanozyme can be used for tumor therapy through starvation, cascade catalytic reactions, ferroptosis, and photothermal manners. This work presents a derivative method that transforms minerals into nanozyme and couples nanozymes for synergistic tumor therapy, shedding light on the rational design of nanozyme composites for biomedicine.
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Nature Communications
·
2022
·Vol. 13
·期 1
·1-13
·
IF 17.694
Xiao Kui,Zhang Niyuan,Li Feifei,Hou Dayong,Zhai Xiaoyi,Xu Wanhai,Wang Gelin,Wang Hao,Zhao Liang
Medicinal applications of gold complexes have recently attracted attention due to their innovative antitumor mechanisms. In this work, two hypercoordinated carbon-centered gold clusters PAA4 and PAA5 are quantitatively synthesized by an intramolecular 6- exo - dig cyclization of polymetalated precursors. The on-bench and in vitro experimental studies demonstrate that the characteristic hypercarbon-tetragold(I) multi-center bonding in PAA4 and PAA5 not only guarantees their stability under common physiological conditions, but also facilitates a glutathione (GSH)-triggered prompt and synergetic release of active Au(I) ions in the GSH-overexpressed and acidic microenvironment of human bladder cancer EJ cells. The instantly massive release of coordination unsaturated Au(I) ions causes the efficient inhibition of thioredoxin reductases and then induces a rapid pro-oxidant response, consequently causing the occurrence of accelerated ferroptosis of EJ cells. As a result, these hypercarbon-centered gold(I) cluster prodrugs show high cytotoxicity to bladder cancer cell lines and thus exhibit a significant inhibition effect towards bladder tumors in vivo. Correlation of the synergetic domino dissociation of carbon-polymetal multi-center bonding in metal clusters with the accelerated ferroptosis of cancer cells provides a strategy for metallo-prodrugs and opens a broader prospect for the biological application of metal cluster compounds.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2022
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IF 4.831
Xiaoyuan Ma,Shan Xu,Liangyu Li,Zhouping Wang
Here, an aptamer-based SERS method for the detection of Staphylococcus aureus ( S. aureus ) without immobilization using [email protected] NPs/slide as enhanced substrate was constructed. ROX-aptamer of S. aureus was modified on the surface of [email protected] NPs/slide through electrostatic interaction. Based on the specific binding effect of the aptamer to S. aureus , the ROX-aptamer fell off from the surface of the substrate, resulting in a decrease of the SERS signal intensity of the substrate. Under the optimal experimental conditions, a good linear relationship was found between SERS intensity at 1500 cm −1 and the logarithm of concentration of S. aureus in the range of 10 2 cfu/mL-10 7 cfu/mL (y = 6623-796lgx, R 2 = 0.994) with a detection limit of 6 cfu/mL. The selectivity analysis revealed that the method had higher selectivity toward the corresponding target. The results for milk sample using the developed SERS method for the detection of S. aureus were similar to those of the plate counting method. The recovery ratio was from 90.60% to 107.26%, indicating the accuracy and reliability of the developed method. This method eliminates the need for bacterial immobilization and improves the convenience and efficiency of detection.
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JOURNAL OF CATALYSIS
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2022
·
IF 8.047
Xiao-Li Hao,Xiao-Shan Chu,Ke-Ling Luo,Wei Li
Atom transfer radical addition (ATRA) is commonly applied to introduce perfluoroalkyl groups into organic molecules, but it often suffers from harsh reaction condition, poor yield and expensive catalyst usage for practical application. Light-induced ATRA reaction is of great significance due to its merits of mild reaction condition and easy operation, but it is generally difficult to achieve high yield because of the lack of ideal photosensitizers. Herein, an Au single-atom sites (SASs) decorated hexagonal cadmium sulfide (CdS) nanocatalyst (Cat CdS-Au ) was synthesized to catalyze ATRA reaction under mild blue-LED-induction. As a result, the synergetic effect of CdS-Au interaction enhanced the optical properties and promoted the host (CdS)-guest (Au) electron transfer, leading to high ATRA yields (88–98%) and excellent selectivity even choosing different alkene substrates, which is remarkably superior over existing reaction systems (60–90% yields) ever reported in literatures. The present work sheds light on realizing high ATRA reaction yield via a green and high-efficient synthetic strategy.
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TALANTA
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2022
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IF 6.556
Yi Wu,Keren Lu,Fubin Pei,Yanghao Yan,Shasha Feng,Qingli Hao,Mingzhu Xia,Wu Lei
The wide clinical application of d -penicillamine (D-PA) makes it inevitably accumulates in the environment, seriously threatening human health and the ecological environment. To better supervisory control D-PA, a highly sensitive and reliable photoelectrochemical (PEC) sensor based on gold nanoparticles (Au NPs) loaded on graphitic carbon nitride sheet and hexagonal NH 2 -UiO-66 composite (g-C 3 N 4 /Au/NH 2 -UiO-66) was synthesized. Tactfully using the strong bonding between D-PA and Au NPs and the effective carrier separation of Z-scheme heterojunction, the designed g-C 3 N 4 /Au/NH 2 -UiO-66 PEC sensor without an extra recognition unit exhibited a selective and sensitive photocurrent to D-PA. With the aid of UV diffuse reflectance spectra (UV-DRs), electron paramagnetic resonance (EPR) characterization, and free radical capture experiments, the electron transfer path of the PEC sensing system was deduced. The proposed g-C 3 N 4 /Au/NH 2 -UiO-66 PEC-based sensor achieved a low detection limit of 0.0046 μM (S/N = 3) with a wide linear response ranging from 10 nM to 400 μM. In addition, its good stability and selectivity also laid a good foundation for practical applications.
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BIOSENSORS & BIOELECTRONICS
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2022
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IF 12.545
Yingzhi Zhang,Jiayuan Fan,Jing Zhao,Zhangrun Xu
Exosomes, carrying specific molecular information of their parent cells, have been regarded as a kind of promising noninvasive biomarker for liquid biopsy. Plentiful fluorescence methods have been proposed for exosome assay. However, most of them are dependent on nucleic acid signal amplification strategies, which require complicated sequence design and experimental operation. Herein, a metal-enhanced fluorescence (MEF) biochip based on shell-isolated [email protected] 2 nanoparticle array was designed for simple and sensitive assay of exosomes. The designed method consists of only two parts: signal conversion and MEF amplification. The conversion of exosome signals to DNA signals was realized by means of chain displacement reaction. The subtle conversion effectively averts the effect of steric hindrance on MEF while amplifying the signal easily for the first time. The MEF biochip based on shell-isolated [email protected] 2 nanoparticle array achieves a second signal amplification in a simple way. Profiting from the two signal amplifications, this strategy displays high sensitivity toward exosomes with a detection limit of 4.5 × 10 3 particles μL −1 . Compared with the result without MEF, the sensitivity is enhanced about thirty times. As far as we know, this is the first attempt for exosome assay by using MEF strategy. In addition to the favorable fluorescence enhancement, both shell-isolated [email protected] 2 nanoparticles and [email protected] 2 nanoparticle array show excellent stability in buffer solutions, which is conducive to practical application. Moreover, the proposed method is able to distinguish breast cancer patients from healthy people, showing its potential for exosome-based liquid biopsy.
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TALANTA
·
2022
·
IF 6.556
Nuo Duan,Tangbin Yao,Changxin Li,Zhouping Wang,Shijia Wu
We designed a SERS-based method to detect β-lactoglobulin (β-Lg), a major allergen of milk, utilizing aptamers to specifically anchor β-Lg. The Au–Ag nanourchins with excellent Raman enhancement effects were prepared and modified with a β-Lg aptamer as the capture substrate. By taking advantage of the hybridization of aptamer and Raman reporter molecule (6-Carboxyl-X-Rhodamine: ROX)-labeled complementary DNA (cDNA), when ROX is close to Au–Ag nanourchins, the SERS signal is enhanced. In the presence of β-Lg, aptamer binding to β-Lg induces the dissociation of ROX-cDNA from Au–Ag nanourchins, thereby decreasing the SERS signal. Under optimal conditions, the present bioassay provides a wide detection range of 10 ng/mL to 1000 ng/mL with a detection limit of 0.07 ng/mL. In addition, the established SERS sensor can selectively and accurately identify β-Lg with recovery rates of 93.25–107.0% in milk samples. It provides great potential as a sensitive and reliable detection method against the growing threat of allergens.
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CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2022
·Vol. 38
·期 4
·941-948
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IF 2.726
Shen Congcong,Chen Yuehua,Feng Beidou,Chi Hongying,Zhang Hua
An ultrasensitive electrochemical biosensor was fabricated for electroanalytical determination of ascorbic acid(AA), dopamine(DA) and uric acid(UA) individually and simultaneously based on polypyrrole hollow nanotubes loaded with Au and Fe 3 O 4 nanoparticles(NPs) uniformly(PPy@Au-Fe 3 O 4 ). The PPy@Au-Fe 3 O 4 nanotubes were synthesized in one-pot using MoO 3 nanorods as templates and the polymerization of Py, the formation of Au and Fe 3 O 4 NPs and the removel of MoO 3 templates took place stimultaneously. Electrochemical studies reveal that PPy@Au-Fe 3 O 4 modified glassy carbon electrode(GCE) possesses excellent electrocatalytic activities toward the oxidation of AA, DA and UA. Their oxidation peak currents increase linearly in the concentration ranges of 1–2000 µmol/L for AA, 0.01–25 and 25–300 µmol/L for DA and 0.1–300 µmol/L for UA. Their detection limit values( S/N =3) were calculated as 0.45, 0.0049, and 0.051 µmol/L for AA, DA and UA in the individual detection. By changing the concentrations simultaneously, the calibration curves showed linearity to 1000, 200, and 200 µmol/L with detection limit of 0.39, 0.0060, and 0.060 µmol/L for AA, DA, and UA, respectively. Finally, the obtained biosensor was successfully applied to the detection of AA, DA, and UA with satisfactory results on actual samples.
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TALANTA
·
2022
·
IF 6.556
Jin Wang,Lin Zhou,Jiali Bei,Qiyuan Zhao,Xue Li,Jiaqi He,Yan Cai,Tingting Chen,Yukou Du,Yong Yao
Pillar [5]arene, a new water-soluble carboxylatopillar [5]arene ammonium salts (WP5), has been employed as the host for complexation of guest molecules. Herein, a visible light driven WP5 functionalized gold nanoparticles (Au@WP5) was fabricated for ultrasensitive photelectrochemical (PEC) detection of caffeic acid (CA). The ultraviolet–visible spectrum characteristics, PEC response results of samples in caffeic acid solution confirm the localized surface plasmon resonance effect of Au NPs and the host-guest interaction between WP5 and CA are responsible for the enhanced PEC sensing performance. Under optimal conditions, the sensitive PEC sensor constructed with Au@WP5 exhibited the concentration linear range from 0.025 μM to 370 μM and a detection limit of 0.01 μM (S/N = 3). Importantly, the good anti-interference ability, stability and reproducibility of the proposed PEC sensor providing the promising detection application of pillar [5]arene functionalized photoactive materials in food and drinks.
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Progress in Natural Science-Materials International
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2022
·
IF 4.269
Xue Li,Jinhua Li,Wentao Yang,Dandan Han,Na Yao,Hongbin Zhao,Xueying Chu,Xuemei Liang,Chunguang Bi,Chunfeng Wang,Guilian Yang
An ultrasensitive and selective sandwich-type fluorescent immunosensor based on fluorescence resonance energy transfer (FRET) between CdSe/ZnS quantum dots (QDs) and Au nanorods (AuNRs) was developed for the rapid detection of porcine reproductive and respiratory syndrome virus (PRRSV). Modified CdSe/ZnS QDs and AuNRs were bioconjugated with primary antibodies M and GP5, respectively. When the target antigen was present, a well-known sandwich-type form CdSe/ZnS QDs-M antibody-antigen-GP5 antibody-AuNRs was produced, and thus reducing the distance between the QDs and NRs. As a result, FRET occurred and the fluorescence intensity of CdSe/ZnS QDs decreased, and this decrease was used to determine the antigen concentration. Ultrasensitive detection of PRRSV at low concentrations in swine serum was achieved with a limit of detection of 0.55 TCID 50 /mL and a linear detection range of 10 1 –3.5 × 10 4 TCID 50 /mL. Therefore, the fluorescent immunosensor is selective and highly sensitive; specifically, it can detect PRRSV at low concentrations in swine serum over a large concentration range. This proposed detection method can facilitate the development of high-performance fluorescent immunosensors for the detection of PRRSV and other antigens.
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