TALANTA
·
2020
·
IF 5.339
Bingxue Hu,Da-Wen Sun,Hongbin Pu,Qingyi Wei
A universal surface-enhanced Raman scattering (SERS) substrate with tuneable plasmonic behaviour and long-term stability was synthesized and characterized, and the substrate was applied in the sensitive detection of fungicide malachite green (MG) in fish fillets. Temperature-responsive poly (N-isopropyl acrylamide) (pNIPAM) template and anisotropic Au nanorods (NRs) were synthesized to prepare pNIPAM @ Au NRs nano-hybrids with dynamically optical and SERS properties and high stability. Results showed that excitation wavelength-dependent SERS efficiency could be tailored by temperature-induced swelling and collapse of the nano-hybrids, thus anticipated optical and SERS properties of the composites could be obtained. Furthermore, the SERS performance, uniformity, long-term stability analysis, and MG in fish evaluation showed that the detection of MG in fish tissues was realized with a limit of detection of 1.58 × 10 −9 M (0.73 ng/g), and the pNIPAM @ Au NRs composites could serve as effective SERS substrates allowing trace level detection of contaminants for the food industry.
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高影响因子
Small
·
2024
·Vol. 21
·期 6
·2409806
·
IF 13
Ruiyuan Zhang,Yu Guo,Chen Huang,Jixiang Fang
The heterogeneity of extracellular vesicles (EVs) surface information represents different functions, which is neglected in previous studies. In this study, a label-free SERS analysis approach is demonstrated to study fundamental EV biological and physical information heterogeneity by matching specific sizes of nano-enhanced particles. This strategy reveals informative, comprehensive, and high-quality SERS spectra of the overall exosome surface, and effectively circumvents the key information loss caused by the spatial resistance of NPs binding to the 293 exosomes' concave structure. The classification of normal and cancerous cell-derived exosomes by PCA method, the accuracy is improved from 91.2% to 95.1% by optimizing sizes of nano-enhanced particles. In addition, stem cell-derived EVs of diverse sizes and morphologies similarly show acuity of spectrum variation to NPs size, which is conductive to qualitative studies. This new strategy will offer a widened in-depth understanding of the surface information, size, and morphology of EVs, which can be applied to the study of biological functions.
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高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2018
·
IF 3.412
Nan Yang,Ting-Ting You,Yu-Kun Gao,Chen-Meng Zhang,Peng-Gang Yin
Surface enhanced Raman scattering (SERS) has been widely used in detection of food safety due to the nondestructive examination property. Here, we reported a flexible SERS film based on a polymer-immobilized gold nanorod polymer metafilm. Polystyrene–polyisoprene–polystyrene (SIS), a transparent and flexible, along with having excellent elasticity, polymer, was chosen as the main support of gold nanorods. A simple phase transfer progress was adopted to mix the gold nanorods with the polymer, which can further be used in most water-insoluble polymers. The SERS film performed satisfactorily while being tested in a series of standard Raman probes, like crystal violet (CV) and malachite green (MG). Moreover, the excellent reproducibility and elastic properties make the film a promising substrate in practical detection. Hence, the MG detection on the fish surface and trace thiram detection on orange pericarp were inspected with detection results of 1 × 10–10 and 1 × 10–6 M, which were below the demand of the National standard of China, exactly matching the realistic application requirements.
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Sensors & Diagnostics
·
2023
·Vol. 2
·期 5
·1292-1301
·
IF 0
Yu Lu,Ruihua Fei,Jiahe Zhang,Geng Zhu,Xiufang Mo,Yu Wan,Yan Huang,Qingjiang Sun,Dianhuai Meng,Xiangwei Zhao
The clinical symptoms of coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), are similar to those of influenza A infection. Therefore, accurate diagnosis of SARS-CoV-2 and influenza A in the early infection stage is essential for appropriate clinical treatment. In this work, a vertical flow assay (VFA) device based on Au@Ag@Au core–shell SERS nanotags and anodic aluminum oxide (AAO) was proposed to diagnose and distinguish between SARS-CoV-2 and influenza A at the same time. Antibodies paired with SARS-CoV-2 and influenza A were immobilized simultaneously on the AAO. In the presence of SARS-CoV-2 or influenza A antigens, sandwich immune-complexes were formed on the AAO and the corresponding SERS signals of nanotags could be detected. The whole detection process took only about 15 minutes, taking comparable or even shorter time than reported POCT antigen assays. The detection limit reached 0.47 pg ml−1 for SARS-CoV-2 nucleocapsid protein (NP) and 0.62 pg ml−1 for influenza A antigen. The clinical feasibility of the proposed VFA device was validated by testing clinical throat swab samples (3 SARS-CoV-2 positive samples and 17 negative samples). Real sample analysis indicated a well-distinguished positive/negative sample group. Conceptually, this VFA device is a potential POCT diagnosis tool for determining if a suspected patient is infected with SARS-CoV-2 or influenza A.
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TALANTA
·
2018
·
IF 4.244
Wen Luo,Miao Chen,Naiying Hao,Xueqian Huang,Xinyi Zhao,Yuqiu Zhu,Hua Yang,Xiaoqing Chen
Surface enhanced Raman scattering (SERS) substrates that can be attached to rough, irregular surfaces and directly collect samples is especially useful for the detection of surface organic residues. Herein, novel AuNPs-pseudo-paper films (APPFs) with uniform structure, flexible properties and wicking capabilities were first fabricated and used as SERS substrate for the sensitive detection of surface pesticides residues . Gold nanoparticles (AuNPs) were in situ synthesized on pseudo-paper films (PPFs) by iterative seeding method to create lots of “hot-spots”, accordingly exhibiting high SERS activity (SERS enhancement factor of 3.02 × 10 6 ). By virtue of polyethylenimine (PEI) grafted onto the dissolved microcrystalline cellulose (MCC), AuCl 4 - and AuNPs can firmly be bonded to the surfaces of PPFs. The prepared APPFs show high reproducibility (relative standard deviation of 6.13%), which is attributed to the uniform surface of the films. The fabricated APPFs SERS substrate allows rapid detection of surface pesticides residues by a facile “swabbing-measure” detection mode avoiding tedious and time-consuming sampling and separation processes. Based on their inherent SERS spectra, thiram , parathion methyl , and malachite green (MG) can be simultaneously detected on apple peel, which demonstrates the potential applicability of this developed protocol for surface organic residues analysis in agriculture and food security.
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MICROCHEMICAL JOURNAL
·
2020
·
IF 3.594
Di Xu,Lingfeng Duan,Wenlin Jia,Guohai Yang,Yingqiu Gu
Noble metal nanoparticle aggregates are attracting increasing interest as SERS active substrates for the ultrasensitive detection of environmental and biological samples. Deposition of noble metal nanoparticles on magnetic metal oxide is an effective way to not only realize the assembly of noble metal particles, but also enrich analytes in highly diluted solution. Herein, Ag@Fe 2 O 3 hybrid was prepared by a simple method and applied for sensitive and recyclable SERS detection. The low detection limit of R6G (LOD = 9.3 × 10 −10 M) and CV (LOD = 1.7 × 10 −9 M) demonstrated the excellent sensitivity for SERS application. The high stability during long-term detection illustrated the super detection capability of the Ag@Fe 2 O 3 hybrid substrate. The synergistic effect of the electromagnetic enhancement originated from nanogaps between densely and closely distributed Ag nanoparticles, the chemical enhancement from semiconductor material of Fe 2 O 3 and the magnetic enrichment of the analyte molecules was account for the excellent SERS performance of the Ag@Fe 2 O 3 substrate. As a result, the Ag@Fe 2 O 3 hybrid substrate was successfully applied to detect bilirubin in human blood and showed excellent detection ability (LOD = 2.3 × 10 −8 M).
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TALANTA
·
2025
·
IF 6.1
Wei Li,Xinrui Wang,Mingze Zhu,Xin Huang,Pacifique Hirwa Umutoni,Ting-Hsuan Chen,Jian Lu,Shih-Chi Chen,Guanming Tan,Bryan P. Yan,Bee Luan Khoo
Chronic inflammation remains a major global health concern, necessitating the development of advanced tools for continuous, precise monitoring. This study introduces a novel, clinically impactful triple-mode probe that integrates colorimetric, fluorescence, and surface-enhanced Raman scattering (SERS) signals, offering unparalleled multimodal detection capabilities. The probe's design leverages europium chelate-doped polystyrene nanoparticles (ECNPs), ensuring minimal signal cross-interference through a long Stokes shift. A key innovation is the development of a multimodal mapping algorithm that seamlessly integrates these optical signals, providing a sensitive and robust platform for biomarker detection with broad dynamic ranges. The probe's clinical relevance is demonstrated by its application in lateral flow assays (LFAs) for detecting C-reactive protein (CRP) levels, achieving a detection limit of 6.31 ng/mL and dynamic ranges from 23.13 to 2000 ng/mL, significantly outperforming single-mode detection methods. In clinical validation using urine samples from 31 patients, the triple-mode LFA showed excellent correlation (0.9377) and agreement (93.55%) with gold standard enzyme-linked immunosorbent assay (ELISA) results. This demonstrates that the proposed multimodal platform offers a highly sensitive, cost-effective, and versatile tool for monitoring inflammation and other disease biomarkers, with substantial potential for clinical applications in diagnostics and disease management.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Xinyu Lan,Guojun Weng,Jianjun Li,Jian Zhu,Junwu Zhao
Surface metal deposition is an important method for tuning the optical property of plasmonic nanoparticles. In this work, we tried to deposit Cu shell on the surface of gold nanorods (Au NRs) by dihydronicotinamide adenine dinucleotide (NADH)-mediated reaction. Cu 2+ were reduced to Cu 0 through the gold-catalyzed oxidization of NADH. The deposition of Cu shell onto the Au NRs surface resulted in the obvious red-shift of longitudinal localized surface plasmon resonance peak. And the red-shift of longitudinal peak increased with the concentrations of NADH and Cu 2+ . The TEM images showed that a transparent layer structure with a different contrast around Au NRs was clearly displayed. And this transparent layer structure with the low contrast was generally considered to be pure Cu shell. However, the results from energy dispersive spectrometer (EDS) and X-ray photoelectron spectroscopy (XPS) indicated that the transparent layer was not just pure Cu composition, but contained NADH/NAD + and Cu 2+ components. This grown complex dielectric shell resulted in the reduced surface enhanced Raman scattering (SERS) activity of Au NRs. Based on the decreased SERS intensity, we demonstrated a SERS method for Cu 2+ detection using R6G as Raman reporter. The decreased SERS intensity had a linear correlation with the concentration of Cu 2+ in the range of 10 −6 –10 −2 M, with a detection limit of 3.89 × 10 −6 M. This study provides new insights about Cu deposition on Au NRs, which will further promote the spectral sensing applications with gold-catalyzed Cu 2+ reduction on plasmonic nanoparticles by NADH.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2025
·
IF 5.4
Li Sun,Zheng Ye,Yuan Zhi,Hua Zhang,Binlin Dou,Yuejin Shan,Jian Chen
In this study, a prospective strategy on seed-mediated growth in vacuum is provided and used to prepare flexible silver nanofilms (AC 0–3 NFs) with high purity, crystallinity and SERS performance. Noteworthy, AC 2 NFs surface is uniformly covered with silver nanoparticles (Ag NPs), following the appearance of numerous nano-gaps between Ag NPs. Through the finite-difference time-domain (FDTD) simulation, it is found that the ultra-small nano-gaps less than 5 nm can generate abundant “hot spots” to amplify the Raman signal. Therefore, the above AC 2 NFs as SERS substrates exhibit superior activity for the SERS detection of thiram in tomato juice, with the limit of detection (LOD) of 10 −15 M and the enhancement factor (EF) of 3.7 × 10 11 . Meanwhile, AC 2 NFs show outstanding reproducibility and stability during the SERS detection. Additionally, the calibration curve is established, by which the thiram (10 −7 to 10 −15 M) can be quantitatively detected. Clearly, the as-prepared flexible AC 2 NFs have great potential in the SERS detection and analysis.
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ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Jiwei Wang,Caiyi Zhang,Zhao Liu,Shibao Li,Ping Ma,Fenglei Gao
A multifunctional theranostic nanosystem that integrates dynamic monitoring and therapeutic functions is necessary for precision tumor medicine. Herein, an entropy-driven self-assembly nanomachine is developed that overcomes the mechanism differences of different diagnostic modes and is applied to miRNA surface-enhanced Raman scattering (SERS)–fluorescence dual-mode dynamic monitoring and synergy phototherapy. It is worth noting that the activated dual-mode theranostic nanosystem (DTN) is capable of tumor in situ fluorescence imaging and SERS absolute quantification of the target. After being internalized into tumor cells, the DTN nanosystem is activated by the DNA cascade chain displacement of the target miR-21, resulting in the secondary release of fluorophores and the assembly of core–satellite structures (CS structures). The coupling of localized surface plasmon resonances (LSPRs) in the CS structure results in the formation of numerous enhanced electric fields (hot spot) in the nanogap of the CS structure. Then the DTN nanosystem greatly improves the sensitivity and repeatability of Raman detection by converting trace targets into numerous adenines residing in the electromagnetic hot spot of the CS structure. Meanwhile, the CS structure and the loaded photosensitizer are used for synergy phototherapy under the guidance of fluorescence imaging. This proposed strategy is confirmed by in vivo and in vitro results, and it provides new ideas for tumor SERS–fluorescence dual-mode diagnosis and effective tumor therapy.
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高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 193
·期 1
·37
·
IF 5.3
Yao Yunfeng,Chen Shuyang,Hu Lingquan,Cheng Cheng,Wu Long,Liu Xing,Zhang Sihang,Jiang Shouxiang
AuNPs-decorated electrospun cellulose acetate nanofiber (AuNPs@CA-NF) SERS substrates were fabricated using a straightforward magnetron sputtering approach. Magnetron sputtering enables not only the uniform deposition of gold nanoparticles on electrospun nanofiber membranes, but also offers advantages such as high speed, scalability for mass production, and the absence of chemical reagents. Consequently, the optimized AuNPs@CA-NF SERS substrate achieved a detection limit of 10 − 11 M for 4-mercaptobenzoic acid (4-MBA), with reliable signal uniformity (RSD = 9.5%) and strong storage stability. The excellent linear relationship between SERS intensity and logarithmic concentrations shows great potential for rapid and quantitative determination of hazardous molecules. Moreover, thanks to the excellent flexibility of the electrospun polymer substrate, the SERS substrate can also detect pesticide residues on irregular fruit surfaces through a simple “adhere-and-read” method. The flexible electrospun polymer SERS substrate achieves a detection limit of 10 − 7 M for thiram, underscoring its strong potential for rapid and ultra-trace monitoring of hazardous substances in food safety.
Section
Graphical abstract
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高影响因子
ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Zixi Huang,Yongqian Lei,Weixin Liang,Yili Cai,Pengran Guo,Jian Sun
Background Pharmaceutical residual such as antibiotics and disinfectants in aquaculture wastewater have significant potential risks for environment and human health. Surface enhanced Raman spectroscopy (SERS) has been widely used for the detection of pharmaceuticals due to its high sensitivity, low cost, and rapidity. However, it is remain a challenge for high-sensitivity SERS detection and accurate identification of complex pollutants. Results Hence, in this work, we developed an aluminum foil (AlF) based SERS detection substrate and established a multilayer perceptron (MLP) deep learning model for the rapid identification of antibiotic components in a mixture. The detection method demonstrated exceptional performance, achieving a high SERS enhancement factor of 4.2 × 10 5 and excellent sensitivity for trace amounts of fleroxacin (2.7 × 10 −8 mol/L), levofloxacin (1.95 × 10 −8 mol/L), and pefloxacin (6.9 × 10 −8 mol/L),sulfadiazine, methylene blue, and malachite green at a concentration of 1 × 10 −8 mol/L can all be detected, the concentrations of the six target compounds and their Raman intensities exhibit a good linear relationship. Moreover, the AlF SERS substrate can be prepared rapidly without adding organic reagents, and it exhibited good reproducibility, with RSD<9.6 %. Additionally, the algorithm model can accurately identify the contaminants mixture of sulfadiazine, methylene blue, and malachite green with a recognition accuracy of 97.8 %, an F1-score of 98.2 %, and a 5-fold cross validation score of 97.4 %, the interpretation analysis using Shapley Additive Explanations (SHAP) reveals that MLP model can specifically concentrate on the distribution of characteristic peaks. Significance The experimental results indicated that the MLP model demonstrated strong performance and good robustness in complex matrices. This research provides a promising detection and identification method for the antibiotics and disinfectants in actual aquaculture wastewater treatment.
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