LANGMUIR
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2025
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IF 3.9
Song Wei,Xueyong Huang,Xuexiao Chen,Lei Zhang
With their excellent optical properties, perovskite thin films demonstrate vast potential for practical applications, ranging from solid-state lighting to advanced display technique. In this work, environmentally benign ionic liquids were employed to synthesize high-quality CsPbBr3 perovskite films. Several carboxylate amine ionic liquids were introduced to prepare CsPbBr3 perovskite thin films. The optimal preparation conditions of the perovskite thin films were screened and predicted by machine learning algorithms. The predicted results are highly consistent with the subsequent verification experiments. The employment of machine learning algorithms has been demonstrated to result in enhanced PL intensity and improved film quality in perovskite thin films. This work provided a new strategy for the utilization of machine learning algorithms in perovskite luminescent materials.
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BIOPROCESS AND BIOSYSTEMS ENGINEERING
·
2025
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IF 3.6
Yue Guoliang,Cai Zhenling,Meng Chun,Mao Yuhong
Efficient immobilized lipase systems are essential for industrial lipid modification, yet the combined effects of enzyme loading, support properties, and post-immobilization stabilization on catalytic performance remain underexplored. In this study, Thermomyces lanuginosus lipase (TLL), Candida antarctica lipase B (CalB), and their mixture (T + C) were respectively immobilized on two structurally distinct commercial supports (A & B)—differing in hydrophobicity, surface area, and pore size—at varied loading densities. Adsorption followed monolayer kinetics, with support B enabling higher enzyme up to 44.1 mg/g wet for T + C. Co-immobilized T + C systems exhibited enhanced catalytic activity compared to individual lipases, particularly in esterification and transesterification, benefiting from the complementary substrate specificities of TLL and CalB. Chemical crosslinking with chitosan and glutaraldehyde significantly reduced lipase desorption and improved thermal stability. The optimized system Cr-B-T + C-M (crosslinked support B-TLL + CalB with medium enzyme loading) efficiently catalyzed fish oil transesterification at 60 °C for 18 h, increasing the EPA + DHA content from 30.5% to 54.8%. All immobilized lipases retained over 80% of their initial catalytic activity after three reuses (60 °C and 18 h). These results highlight the synergistic benefits of co-immobilization and crosslinking on tailored supports, offering a versatile strategy for developing stable and efficient lipase biocatalysts for high-value lipid processing.
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TALANTA
·
2024
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IF 5.6
Lichen Gou,Ling Li,Siqi Wei,Yunfei Tian,Xiandeng Hou,Li Wu
Herein, the surface-enhanced Raman scattering (SERS) platform was combined with an azo coupling reaction and an aluminum alloy covered with a hydrophobic layer of praseodymium oxide and stearic acid complexes for the detection of histamine. The praseodymium oxide on aluminum alloy was successfully synthesized by the rare-earth-salt-solution boiling bath method and modified by stearic acid. Its surface exhibits a water contact angle (WCA) of 125.0°. Through the azo derivatization reaction with 3-amino-5-mercapto-1,2,4-triazole (AMTA) diazonium salts, histamine can be converted into the derivatization product with higher Raman activity. The mixture of the derivatization product and β-cyclodextrin-modified Ag nanoparticles (β-CD-AgNPs) were dropped onto the surface of an aluminum alloy covered with a hydrophobic layer of praseodymium oxide and stearic acid complexes, and dried for SERS measurement. The intensity ratio between the SERS peaks at 1246 cm −1 and 1104 cm −1 (I 1246 /I 1104 ) of the derivatization product was used for the quantification of histamine. Under the selected conditions, the limit of detection (LOD) and the limit of quantification (LOQ) for this method were 7.2 nM (S/N = 3) and 24 nM (S/N = 10), respectively. The relative standard deviation (RSD) of this method for the determination of 1 μM histamine was 6.1 % (n = 20). The method was also successfully used for the determination of histamine in fish samples with recoveries ranging from 92 % to 111 %. The present method is simple, sensitive, reliable, and may provide a new approach for preparing the composite hydrophobic layer that can enhance SERS signals through hydrophobic condensation effect. Meanwhile, it may have a promising future in the determination of small molecular compounds containing an imidazole ring.
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ANALYTICAL LETTERS
·
2024
·
IF 1.6
Jingjing Gu,Chunyang Chen,Tingting Liu,Yanyan Li,Xuedong Wang
Here is reported effervescent reaction-enhanced switchable hydrophilic-hydrophobic solvent-based salting-out microextraction (ERSSM) with high-performance liquid chromatography with photodiode array detection (HPLC-PDA) for the preconcentration, extraction, and trace-level determination of polybrominated diphenyl ethers (PBDEs) in milk and water. The important variables were rigorously optimized to be 150 µL of hexanoic acid as the extraction solvent, 400 µL of Na2CO3 as the alkaline source, 350 µL of H2SO4 solution as the acid source, and NaCl as the salting-out agent. The optimized conditions provided a linear range from 0.2 to 50 µg L−1 with limits of detection and quantitation from 0.026 to 0.085 µg L−1 and 0.087 to 0.28 µg L−1 for six PBDEs. The intra-day and inter-day precision values were from 2.48% to 6.75%, and the fortified recoveries for PBDEs were between 78.06% and 103.07% in water and milk samples. The developed procedure avoids the use of a traditional organic disperser and mechanical mixing allowing the determination of PBDEs outdoors by synchronous extraction method and high efficiency for the analytes. These advantages suggest a high potential for the routine monitoring of PBDEs in liquid food and environmental samples.
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TALANTA
·
2025
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IF 6.1
Yuxin Wang,Yulin Wang,Jizhen Fu,Suzhen Li,Yawen Zhang,Xiaojiao Zheng,Jin Liu,Li Li,Xu Jing
In this study, a rapid and simple semi-automatic emulsification liquid-liquid microextraction technique based on deep eutectic solvents was developed for determining strobilurin fungicides in food samples using high-performance liquid chromatography. Novel deep eutectic solvents formed as extractants by combining quaternary phosphates and fatty acids, eliminating the need for toxic solvents in liquid-phase microextraction. The extraction process was facilitated by an electric micropipette, which emulsified and injected the deep eutectic solvent-water mixture directly into the sample. The semi-automated approach enhanced extraction speed and efficiency while minimizing manual error. The sample and deep eutectic solvent mixture were emulsified immediately and separated within 15 min of standing. Only an electronic micropipette was used to achieve extraction and separation. The method demonstrated a linear range of 2–200 μg L −1 with R 2 > 0.996 and a limit of detection of 0.6 μg L −1 . Recovery ranged from 73.0 % to 100.1 %, with relative standard deviations from 0.4 % to 6.1 %. The greenness of the method was validated through multiple assessment tools, including the Green Analytical Procedure Index, Analytical GREEnness metric for sample preparation, and Sample Preparation Metric of Sustainability. The emulsification liquid-liquid microextraction procedure, based on innovative solvents and electric micropipette technology, proved successful for application in water, juice, and vinegar samples and showed potential for broader use in pesticide residue analysis in food.
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FOOD CHEMISTRY
·
2025
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IF 9.8
Ruili Ma,Weiguo Wu,Yikang Shu,Ran Gao,Ziyu Wang,Daoquan Tang,Yan Du,Shuai Ji
This paper reports a novel method for simultaneous extraction and in situ separation of flavonoids and alkaloids from mulberry leaves using a pH-responsive deep eutectic solvent (DES)/water system. Five novel DESs were synthesized, and isoquercitrin and deoxynojirimycin were respectively selected as the representative compounds of flavonoids and alkaloids to evaluate the extraction and separation efficiency. The optimal DES, namely [HexA (hexanoic acid)][MPD (2-methyl-2,4-pentanediol)], was identified, and the extraction and in situ separation conditions were optimized. After extraction and pH-induced phase separation, flavonoids were mainly enriched in the DES phase with alkaloids in the H 2 O phase, and the distribution ratios were 6.69:1 and 1:9 ([HexA][MPD]:H 2 O), respectively. Compared to traditional solvents, the [HexA][MPD]/H 2 O system showed higher extraction efficiency of flavonoids and alkaloids with higher purity. Moreover, [HexA][MPD] possessed excellent reusability. This study demonstrates the application of pH-responsive DES/H 2 O systems for simultaneous extraction and separation of natural products for the first time.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
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IF 8.5
Xinru He,Zhanpeng Zhang,Xuan Xuan,Tingyuan Tan,Jiaxuan Sun,Biao Wang,Yu Tian,Heng Chen
The application of chitosan in packaging has always been limited due to its brittle and hygroscopic nature. In this study, hydrophobic short-chain fatty acids (SCFAs) were utilized to modify chitosan to overcome this issue. For the first time, hydrophobic SCFAs, typically hexanoic acid and its homologs, were found to be able to dissolve chitosan in water as well as its hydrophilic analog. After water was removed through evaporation, hydrophobic SCFA-modified chitosan films were obtained. The results of mechanical testing and DSC analysis confirmed that these hydrophobic SCFAs could effectively plasticize the chitosan film. Moreover, water contact angle measurements revealed that the hydrophilicity of these plasticized chitosan films was significantly reduced. The findings from the moisture uptake experiments indicated that linear hydrophobic SCFAs reduced the hygroscopicity of the chitosan film. Additionally, the crystal structures, thermal and gas barrier properties, and antibacterial activities of these hydrophobic SCFA-modified films were systematically examined and compared with those of hydrophilic SCFA-modified chitosan. This study presents an innovative and practical method for modifying chitosan films by regulating the structure of the cosolvent, thereby facilitating the real-world application of chitosan-based food packaging materials.
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ULTRASONICS SONOCHEMISTRY
·
2024
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IF 8.7
Yufei Zhang,Yundong Lai,Mingming Zheng
A novel approach to ultrasound-assisted Pickering interfacial biocatalysis (PIB) has been proposed and implemented for the efficient enzymatic transesterification production of vitamin A fatty acid esters. This is the first instance of exploiting the synergistic effect of ultrasound and the bifunctional modification of enzyme supports to accelerate biocatalytic performance in PIB systems. The optimal conditions were determined to be ultrasound power of 70 W, on/off time of 5 s/5 s, substrate molar ratio of 1:1, enzyme addition of 2 %, and a volume ratio of n-hexane to PBS of 3:1, a temperature of 40 °C, and a time of 30 min. The application of ultrasound technology not only improved lipase activity but also allowed for a reduction in emulsion droplet size to enhance interfacial mass transfer. Bifunctional modification of silica-based supports enhanced stability of immobilized enzymes by increasing hydrogen bonding while maintaining the active interface microenvironment. Compared with a non-ultrasound-assisted PIB system stabilized by mono-modified immobilized enzyme particles, the catalytic efficacy (CE) of the novel system reached 8.18 mmol g −1 min −1 , which was enhanced by 3.33-fold, while the interfacial area was found to have increased by 17.5-fold. The results facilitated the conversion of vitamin A palmitate (VAP), vitamin A oleate (VAO), vitamin A linoleate (VAL), and vitamin A linolenate (VALn), with conversion rates of approximately 98.2 %, 97.4 %, 96.1 %, and 94.7 %, respectively. This represents the most efficient example that has been reported to our knowledge. Furthermore, the system demonstrated improved reusability, with a conversion rate of 62.1 % maintained even after 10 cycles. The findings presented in this paper provide valuable insights into an efficient and conveniently promising protocol for the development of PIB systems.
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LANGMUIR
·
2024
·
IF 3.7
Shaoyang Liu,Jie Qi,Yuzhe Li,Ruiyao Wang,Li Wang,Hongsheng Lu,Zheng Zhang
The reversible shift in polarity or hydrophilicity/hydrophobicity of switchable solvents greatly simplifies the recovery capacity in extraction applications. However, the environmental and economical advantages of switchable solvents are not significant. In this work, we designed three pH-responsive natural deep eutectic solvents (NADESs) by combining the pH-switchable solvent fatty acids with the nonswitchable solvent ethyl lactate (EL), followed by the exploration of the solubilization and separation performance of these NADESs for petroleum hydrocarbons. EL can be miscible in fatty acids and water; however, when in contact with both at the same time, EL binds to fatty acids through stronger intermolecular hydrogen bonds, whereas when fatty acids are deprotonated to fatty acid salts, EL can bind to water. The deprotonation/protonation of fatty acids could reversibly change the NADES hydrophilicity, and the recovery of NADES HA/EL could exceed 95% after three cycles. Furthermore, after extractive separation of simulated oils of differing complexity, NADES HA/EL was selected as the best extractant. Compared with the extraction of oil sands with a single solvent, NADES provides better wetting of the sand surface, better stripping efficiency of the heavy components that adhere to the surface of oil sands, and better dispersion of the stripped petroleum hydrocarbons. Petroleum hydrocarbons can be separated by NaOH-induced hydrophilic changes in NADES, which can be regenerated upon the addition of HCl. The recovered NADES showed good reusability in the cleaning of oil sands. The oil removal rates were 96.9%, 94.4%, and 91.9% after three cycles of cleaning with NADES at 25 °C. This method is expected to expand the application of nonswitchable solvents in sustainable extraction.
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NEUROGASTROENTEROLOGY AND MOTILITY
·
2024
·
IF 3.5
Lei Chen,Xiangbing Chang,Chuntao Wu,Guofu Luo,Peifeng Zhang,Wei Tian
Background The traditional herbal medicine Atractylodes macrocephala Koidz. ( A. macrocephala ) is commonly utilized for alleviating symptoms associated with spleen deficiency, abdominal distension, diarrhea, and constipation. These pharmacological effects are attributed to a variety of active constituents. However, the specific bioactive compounds responsible for promoting defecation and gastrointestinal transit in A. macrocephala remain unidentified. Methods The primary polysaccharide characteristics of PAMK was elucidated by HPLC, FT-IR, and HGPGC. Efficacy of PAMK (0.07, 0.14, and 0.28 mg/g) on mice was evaluated in a spleen deficiency constipation mouse model by analyzing stool parameters, constipation-related physiological indexes, and SCFAs. The expression levels of 5-HT 3 R, 5-HT 4 R, and related receptor genes were examined by RT-qPCR, and neurotransmitters were examined using ELISA. Finally, the diversity of gut microbiota was analyzed with 16S rDNA sequencing. Key Results The results showed that PAMK significantly reduced the gastrointestinal transport time and increased the number of fecal pellets and fecal water content in spleen deficiency constipation model mice. PAMK kept the balance of 5-HT, SCFAs, TPH-1, SERT, CgA, and neurotransmitter levels (VIP, SP, MTL) in mice colon. In addition, PAMK could regulate the abundance of gut microbiota such as Alistopes , Bacteroides , and Odoribacter in spleen deficiency constipation model mice gut. Conclusions and Inferences It can be concluded that PAMK effectively ameliorated the symptoms of spleen deficiency constipation in mice by modulating the expression of 5-HT and its associated receptors. The underlying mechanism was elucidated, providing a solid theoretical foundation for the therapeutic application of A. macrocephala in treating spleen deficiency constipation and offering potential for developing novel approaches to address this condition.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
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IF 4.6
Yu Cheng,Zhenning Yan,Liuyang Hu
The interactions of three berberine mid-chain fatty acid salts ([BBR][C n ], n = 6, 7, 8) with lysozyme (Lyz) are investigated in detail using multi-spectroscopic and molecular docking techniques. Steady-state fluorescence and UV–visible absorption experiments suggest that the binding mechanism of [BBR][C n ] on Lyz is a static quenching with a binding ratio of 1:1. The compound [BBR][C n ] exhibits a moderate binding affinity toward Lyz. The findings from UV–vis, synchronous fluorescence, three-dimensional fluorescence and FT-IR spectral measurements manifested the alternation in the secondary structure, microenvironment and conformation of Lyz by the binding of [BBR][C n ]. The α-helix content of Lyz declines, while the β-sheet content increases. The theory of Förster resonance energy transfer (FRET) was employed to measure the molecular distance between Lyz and [BBR][C n ]. Thermodynamic parameters and molecular docking results indicate that hydrophobic interaction, van der Waals force, and hydrogen bond are the primary driving forces during the binding process. The binding event enhances the esterase-like activity of Lyz. Furthermore, the binding affinity is influenced by environmental factors such as pH and temperature, as well as the anionic alkyl chain length of [BBR][C n ].
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危险品化学品经营许可证（带存储）