FOOD CHEMISTRY
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2026
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IF 10.4
Xiang Luo,Yang Wang,Man Zhou,Qiaolan Sun,Xue Wang,Xiaojie Yu,Li Chen,Hua Yang,Cunshan Zhou
Rebaudioside M is a desirable zero-calorie sweetener, but its low natural abundance limits industrial application. Here, enzymatic conversion of Rebaudioside D was intensified through host engineering, cascade biocatalysis, and substrate feeding. Expression of the glycosyltransferase in a eukaryotic host increased the soluble enzyme fraction from 18% to 92% and improved specific activity by 1.46-fold compared with the bacterial system. Coupling with sucrose synthase enabled in situ regeneration of the sugar donor from inexpensive sucrose. Kinetic analysis identified substrate depletion as the dominant bottleneck in batch reactions. A fed-batch feeding strategy increased the product titer to 28.38 g L −1 with 91.6% conversion, representing a 3.99-fold improvement over batch mode. This integrated strategy provides a practical basis for cost-efficient production of next-generation natural sweeteners.
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FOOD CHEMISTRY
·
2026
·
IF 10.4
Yue Liu,Xueer Wang,Xinyan Li,Yushu Ma,Tianqin Li,Yaodong Guo,Zhouli Wang
Polysaccharides from Lentinula edodes ( L. edodes ) exhibit antioxidant and immunomodulatory activities, but conventional extraction is limited by low efficiency and potential loss of bioactivity. This study evaluated the effects of intense pulsed light (IPL)-assisted extraction on the physicochemical, structural, rheological, and antioxidant properties of L. edodes polysaccharides (LNTs). The ternary IUEH method increased polysaccharide yield (92.7%) and uronic acid content (25.96%) compared with hot water extraction, while improving protein removal and preserving acidic fractions. IPL induced partial depolymerization without altering the β-(1 → 3)-glucan backbones, leading to macromolecular reorganization and improved thermal stability. IPL-treated LNTs exhibited increased pseudoplasticity and elasticity. In simulated digestion, IUEH-derived LNTs showed higher reducing sugar release and DPPH radical scavenging activity (70.55%). These findings demonstrate that IPL-assisted extraction efficiently produces structurally optimized LNTs with improved functional properties, supporting their potential application as functional food ingredients.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2026
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IF 6.7
Rui-Lin Zhang,Jia-Qing Wang,Sara Taha Abdelkhalek,Shan-Cheng Yi,Yuping Zha,Man-Qun Wang
The olfactory system is pivotal for insect behavior, with odorant-binding proteins (OBPs) serving as primary detectors for semiochemicals. Previous studies demonstrated that DhelOBP4 in Dastarcus helophoroides is essential for detecting herbivore-induced plant volatiles (HIPVs). Here, we demonstrated through dual-luciferase reporter assays, yeast one-hybrid (Y1H) assays, and electrophoretic mobility shift assays (EMSA) that transcription factors BarH1/2 directly activate DhelOBP4 expression by binding to its promoter. RNAi knockdown revealed sex-specific regulation: BarH1 silencing significantly reduced DhelOBP4 expression only in females, whereas BarH2 knockdown affected both sexes. Y-tube olfactometer assays showed that BarH1 depletion abolished female attraction to α-pinene and 2-undecyloxy-1-ethanol, as well as male attraction to α-pinene. BarH2 knockdown eliminated attraction in both sexes and reversed the female response to α-pinene from attraction to repulsion. These findings establish DhelOBP4 as a key detector of these volatiles, under sex-specific regulation by BarH1/2 to modulate behavior, revealing novel mechanisms in tritrophic interactions.
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FOOD CHEMISTRY
·
2026
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IF 10.4
Jingbin Shi,Hongyuan Guo,Hao Wu,Xuedong Jia,Runying Chai,Yujue Feng,Suozhu Wu
Norfloxacin (NOR) is a fluoroquinolone antibiotic, and its long-term intake poses serious threats to human health. Therefore, sensitive determination of NOR is of great significance. This work develops a molecularly imprinted electrochemical sensor (MIES) for detecting NOR based on molecularly imprinted polypyrrole/carboxylated multi-walled carbon nanotubes-chitosan/glassy carbon electrode (MIP/cMWCNT-CS/GCE). The cMWCNT-CS serves as a conductive layer for signal amplification, while MIP acts as the recognition element for specific identification of NOR. NOR is indirectly detected by the reduction peak current of potassium ferricyanide probe . This MIES exhibits an ultra-wide linear range (1.00 × 10 −13 –1.00 × 10 −5 M) and an ultra-low detection limit (1.78 × 10 −14 M) with rapid response (adsorption time 4 min), high selectivity, and excellent reproducibility. Furthermore, the MIES is successfully applied to determine NOR in honey, milk, and tap water samples with satisfactory recoveries (87.30% ∼ 121.00%). This work provides a promising analytical avenue for rapid and ultrasensitive monitoring of NOR in food and environmental samples.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
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IF 8.7
Yunhao Song,Yihui Lin,Shuangshaung Li,Xueping Huang,Na Wen,Jinlin Long,Ying Wang,Pengzhao Wang,Tengfei Lin,Xunbin Yu,Shiyun Lu
Wound infections caused by bacterial invasion significantly impair tissue regeneration and prolong healing. While injectable hydrogels show promise for wound management, their clinical application is limited by pH sensitivity in infected wound microenvironments. In this study, we successfully developed a novel pH-independent antibacterial hydrogel (FHAg) via hydroxyethyl cellulose (HEC)-guided self-assembly of Fmoc-Glu-OMe and in situ synthesized silver nanoparticles (AgNPs). The HEC matrix provides a stable structural scaffold for the hydrogel, promotes π-π stacking self-assembly, and confers exceptional stability within a broad pH range (3.0–7.4), effectively overcoming the pH-sensitivity limitation of conventional hydrogels in acidic wound microenvironments. The hydrogel exhibited exceptional biocompatibility, potent antibacterial and antibiofilm activity in vitro. In vivo MRSA -infected rat wound model revealed two key advantages: 99.9% bacterial eradication efficacy and accelerated wound healing within 12 days. This pH-resilient hydrogel platform addresses critical challenges in infected wound care, showing strong potential for clinical translation.
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Analytical Methods
·
2026
·
IF 3
Hechen Lu,Yaohui Xue,Jinhua Li,Ming Ma,Qingwei Song,Sihua Qian,Jian Li,Yuhui Wang
Hormones residual especially in aquatic products has become a serious threat to food safety. The development of sensitive, affordable, on-site, quick detection methods for hormones residual still faces great challenges. Herein, taken methyltestosterone (MT) as a representative analyte, a competitive lateral flow immunoassay (LFIA) platform was constructed using ultrabright lanthanide-doped luminescent nanoparticles i.e. YVO4: Eu as the label probes. The YVO4: Eu NPs were obtained by a facile hydrothermal method using lanthanide ions, sodium orthovanadate, and polyacrylic acid, as the precursor and surface modifier, respectively. Thanks to the advantages of high fluorescence quantum yield, abundant surface functional groups, good dispersity and water-solubility, the harvested YVO4: Eu NPs would be a competitive and promising label probe in LFIA. The YVO4: Eu LFIA test strips have shown a detection limit i.e. 0.1 ng/mL, which was about 1 or 2 orders of magnitude lower than most reported methods. Furthermore, the proposed test strips were successfully applied for the quantitative, accurate, and quick screening of MT in fish samples. This work reported a competitive luminescent label probe for food safety and quality control.
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JOURNAL OF MEMBRANE SCIENCE
·
2026
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IF 8.8
Wenji Zheng,Yuliang Tao,Fengting Yao,Zhixu Yun,Miao Yu,Xiaobin Jiang,Gaohong He
Metal organic frameworks (MOFs)-based mixed matrix membranes (MMMs) exhibit great prospect for He/CH 4 separation. However, it is still restricted by interfacial incompatibility-induced low filler loading and selectivity loss. This study proposed an interface fusion strategy by 6FDA-TFMB (PI) functionalized CF 3 -UiO-66 through NH 2 -UiO-66 bridging to achieve dual regulation of interface compatibility and molecular sieving. Crucially, this interface fusion strategy allowed a substantial increase in filler loading to 20 wt%, representing a 100% improvement over unfunctionalized CF 3 -UiO-66. The covalent bond formed between the amino group and the dianhydride improved the interfacial compatibility. The –CF 3 group on the UiO-66 reduced the pore size of UiO-66 and weaken the adsorption of CH 4 . The dual synergistic effect made the resultant MMMs exhibited excellent He permeability (165.2 Barrer) and He/CH 4 selectivity (394.1), representing improvements of 81.14% and 319.7%, respectively, compared with pristine 6FDA-TFMB membrane, approaching to the 2008 Robenson upper bound, surpassing most of the reported PI based membranes for He/CH 4 separation. This synergistic architecture not only validated the interface fusion engineering but also provided a foundation for designing efficient He/CH 4 separation materials with tailored microscopic properties.
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ANALYTICA CHIMICA ACTA
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2026
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IF 6.1
Shanshan Tao,Ruijia Hu,Xinyi Zhang,Chang Liu,Suqin Wang,Hongbo Li,Ruqin Yu
The residues of organophosphorus pesticides (OPs) in the environment and crops, resulting from irrational use will cause irreversible damage to the human nervous system by hindering acetylcholinesterase (AChE) activities. This study developed a novel proportional fluorescence sensing system based on UiO-66-NH 2 coated MnO 2 and Ampliflu Red (AR). In this sensor system, manganese dioxide nanosheets have both fluorescence quenching and catalytic functions. Based on the core mechanism of selective decomposition by reducing agents, it can respond to various OPs and enable multi-target detection of reducing biomolecules such as glutathione (GSH), cysteine (Cys), and ascorbic acid (AA). By means of principal component analysis (PCA), the sensor can effectively distinguish among GSH, Cys, and AA, achieving specific recognition of multiple targets. Under optimal conditions, the detection linear range for parathion is 1×10 -6 to 9×10 -1 mg/mL, with a detection limit as low as 0.33×10 -6 mg/mL; The linear ranges for GSH, Cys, and AA detection are 1–100 μM, 30–90 μM, and 1–50 μM, respectively, with detection limits of 0.33 μM, 10 μM, and 0.33 μM, respectively. This method exhibits high accuracy, facile operation, and favorable stability. It has been successfully implemented for the detection of tap water and 1% human serum samples. This makes the research findings more intuitive and clear. In addition, this research provides a feasible approach for developing diversified sensors based on functionalized metal-organic frameworks (MOFs), demonstrating strong potential for applications in environmental monitoring and bioanalysis.
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JOURNAL OF MEMBRANE SCIENCE
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2026
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IF 8.8
Hui Wen,Zhiyu Liu,Wei Qu,J. Paul Chen
Reactive nanofiltration (NF) systems that couple polyamide NF (PA-NF) with advanced oxidation processes reportedly offer a promising route for simultaneous degradation of emerging organic pollutants and desalination. However, their performance is constrained by limited catalytic activity, mass-transfer barriers, and trade-off between permeability–selectivity. Here, we develop a high-performance catalytic PA-NF membrane by introducing a layered double hydroxide (LDH) interlayer with the aim on creation of nanoconfined spaces within the internal membrane structure for enhancement in oxidant activation and mass transport. It was found that the interfacial polymerization by restricting amine diffusion can effectively be regulated, yielding a thin, defect-free PA layer enabling fast water transport and precise ion selectivity. The nanoconfinement with peroxymonosulfate markedly boosts oxidant delivery and electron-transfer efficiency, accelerating radical generation. Hence, the catalytic LDH-interlayered PA-NF membrane exhibited high water permeance (34.8 L/(m 2 ·h·bar)) and excellent ion selectivity, while simultaneously achieving nearly 100% atrazine degradation with a rate constant of 0.034 min −1 , far outperforming non-confined systems, even under natural organic matter and ionic interference. This work would provide a better solution for design of cost-effective materials for next-generation reactive NF technologies and sustainable water and wastewater treatment.
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RSC Advances
·
2026
·Vol. 16
·期 11
·10243-10254
·
IF 6.1
Wenhao Liu,Yanfeng Zhu,Zhihao Liu,Jianxia Gou
Marine corrosion poses a critical threat to the safety of offshore oil and gas facilities. To address the intrinsic drawbacks of pristine TiO2, ZnS/CdS/Ti3+-TiO2 nanotube composites were rationally fabricated via a synergistic strategy combining electrochemical anodization and successive ionic layer adsorption and reaction (SILAR) techniques. Structural and optical characterization confirmed that the as-synthesized composites feature a well-constructed cascade band alignment, which efficiently mitigates the recombination of photogenerated electron–hole pairs. Accordingly, the optimized photoanode delivered a high photocurrent density of 279 µA cm−2. When coupled with 316 stainless steel (316 SS), the composite exhibited a pronounced photo-induced potential of −0.89 V (vs. SCE), achieving a remarkable performance enhancement over pristine TiO2. This enhanced photocathodic protection originates from the synergistic interplay between Ti3+ self-doping and the ZnS/CdS heterojunction: Ti3+ self-doping introduces abundant electron donors to enhance the electrical conductivity of the composite, whereas the ZnS/CdS heterojunction broadens the visible-light absorption range and accelerates the separation and directional migration of photogenerated charge carriers.
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FOOD RESEARCH INTERNATIONAL
·
2026
·
IF 8.8
Jian Li,Yue-Wei Huang,Yi-Tong Li,Shi-Yin Luo,Wei-Yan Xiong,Jin-Hua Wang,Jun-Hu Cheng,Ting-Shi He,Xin-An Zeng
The impact of pulsed electric field (PEF) on the formation of acrylamide (AA) in air-fired French fries was investigated in this study. The results indicated that electric field intensity, pulse width, and cooking parameters significantly influence the formation of acrylamide in air-fried French fries. Compared with the traditional blanching treatment, PEF treatment more effectively inhibits AA formation and improves the cooking quality of French fries. Under optimal conditions, the AA content in the PEF-treated samples was reduced by 15.60% and 26.71% compared with that in the blanched and untreated groups, respectively. The significant suppression of AA formation may be closely associated with the ability of PEF to modify the microstructure of potato tissues and promote the leaching of AA precursors. PEF treatment did not induce significant gelatinization of potato starch. Instead, it caused electroporation of potato cells through polarization effects. This structural change enhanced heat and mass transfer efficiency during cooking, reduced processing time, and facilitated the continuous removal of key AA precursors, such as reducing sugars and asparagine. These structural and process modifications collectively suppress the Maillard reaction, thereby reducing AA generation. Furthermore, as a pretreatment method, PEF exhibits superior processing efficiency and lower energy consumption compared to blanching. In conclusion, PEF treatment can significantly decrease AA levels in air-fried French fries, showing great potential for producing healthier air-fried foods.
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CHEMICAL ENGINEERING JOURNAL
·
2026
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IF 12.5
Yongkang Cui,Xinyue Chen,Xiaokai Gu,Dongyan Zhao,Yilun Zhou,Zhonghao Wang,Shujuan Wang
Amine-based CO 2 capture is a mature and commercially established technology. High-temperature regeneration causes amine thermal degradation, decreasing capture performance. The thermal stability of four long-chain triamines (N-C-C-C-N-C-C-C-N backbone) was evaluated and the inhibitors were screened. Degradation rates were monitored by gas chromatography and degradation products were characterized using ion chromatography and gas chromatography – mass spectrometry. Quantum chemical calculations and ReaxFF molecular dynamics simulations were employed to elucidate the degradation and inhibition mechanisms. The results indicate that triamines degrade rapidly via a radical-based mechanism. Formate and acetate were identified as the major anionic products, while low-molecular-weight fragments dominated the organic products. Under the tested conditions, the degraded solutions shows slight losses in CO 2 capture performance, but the performance losses were limited. Longer chains decrease the bond dissociation energy of C N and C C bonds and degradation is initiated by homolytic scission of C H and C N bonds, generating radical species and triggering subsequent radical reactions. The addition of 2-ethyl-4-methylimidazole enabled triamines to maintain a residual amine ratio of 80% even after thermal treatment at 423.15 K for 6 days by enhancing local electron density and forming stable non-covalent interactions with the triamines. This work provides insights for the development of high-stability amine-based solutions.
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危险品化学品经营许可证（带存储）