Nature Communications
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2026
·Vol. 17
·期 1
·2565
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IF 18.1
He Mingbin,Wang Weiwei,Zhou Haiwu,Liu Cong,Zhao Chunbei,Li Jian,Han Yuewen,Qin Yali,Chen Mingzhou
Pyroptosis plays a crucial role in immune defense against infections and endogenous threats by eliminating harmful cells and modulating the immune response through inflammation. However, the natural activation of pyroptosis involves intricate signaling pathways, posing significant challenges for its artificial manipulation in research and therapies. Here, we present DAMAGE ( D e a th Ma nipulation Ge ne), an innovative system that integrates gasdermins within the type III-E CRISPR framework, enabling the specific recognition of target RNA (tgRNA) and triggering pyroptosis. This mechanism allows DAMAGE to selectively identify and eliminate virus-infected, cancerous, and senescent cells, all of which exhibit altered RNA transcriptomes. Additionally, DAMAGE exhibits considerable promise as a platform for mRNA-LNP therapy. Our study highlights the potential of this CRISPR-based system in the controllable induction of pyroptosis, offering an innovative therapeutic strategy for treating RNA-heterogeneous diseases.
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JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
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2026
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IF 4
Nan Xiang,Xinbo Guo
BACKGROUND Maize ( Zea mays L.), as the most widely cultivated cereal and a dietary cornerstone, provides not only carbohydrates but also essential micronutrients critical for human health, including vitamin C, vitamin E, and carotenoids. However, conventional maize varieties typically exhibit suboptimal levels of these compounds. This study investigated the dynamic accumulation of vitamin C, vitamin E, and carotenoids, along with their transcriptional regulation, during early kernel development (9–15 days after pollination (DAP)) in maize. RESULTS Combined metabolite and transcriptome analyses showed a 23-fold increase in vitamin C content, associated with enhanced expression of the gene VTC2 . Id1 and AGAMOUS-like MADS-box transcription factors (TFs) are putative regulators, targeting the promoters of GalDH and VTC2 to regulate vitamin C biosynthesis. Vitamin E showed isomer-specific fluctuations during early kernel development, mainly associated with the expression of VTE1 , VTE2 , and VTE4 , and potentially regulated by knotted 1 and the AGAMOUS-like MADS-box TFs via VTE2 and VTE3 , respectively. Total carotenoid content tripled, with zeaxanthin becoming dominant by 15 DAP, driven by upregulated PSY1 and CHYB-1 . ERF003 was identified as a candidate regulator of PSY3 . CONCLUSION The increases in vitamin C and carotenoid content, and the fluctuation of vitamin E isomers during early maize kernel development, were associated with the modulation of key genes and putative TFs. These findings reveal theoretical insights and provide candidate genetic targets for future breeding efforts aimed at enhancing micronutrient levels in maize. © 2026 Society of Chemical Industry.
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CHEMICAL ENGINEERING JOURNAL
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2026
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IF 12.5
Hao Chen,Donghua Yang,Yunhao Hou,Aiguo Feng,Xiyong Chen,Kai Chen,Xinpeng Wang
This report presents the first application of an amide linkage strategy in pyrene-based imine COFs. • Amide groups enhance the polarity and coordination ability of TFPPY-DA-COF channels. • The TFPPY-DA-COF achieved an Au adsorption capacity of 2040 mg g −1 . Pyrene-based covalent organic frameworks (COFs) have received considerable attention for gold recovery, yet challenges remain in terms of adsorption capacity and economic cost. Herein, we initially employed an amide linkage strategy to construct a pyrene-based imine COF for gold recovery. The introduction of amide groups enhanced the polarity and coordination ability of the pore walls, enabling amide-linked COF to exhibit an Au uptake of 2040 mg g −1 , which is approximately twice that of its non-amide counterpart, and superior to most previously reported pyrene-based COFs. Moreover amide-linked COF also demonstrates excellent selectivity (distribution coefficient for Au exceeding 2.74 × 10 5 ; maximum separation factor > 6000) and outstanding performance in practical applications. Notably, the ligands used to synthesize both pyrene-based COFs are low-cost, and the captured Au particles can reach micrometer-scale sizes, which facilitates their separation and recovery. Further performance tests, combined with characterization and DFT calculations, reveal that the coordination of Au ions by the carbonyl groups in the amide linkages plays a dominant role during adsorption, and the reduction effect of the protonated imine bonds also contributes to improving the adsorption performance. Overall, this work provides a new approach for using COF materials to selectively capture and recover gold, offering potential for practical applications. Download: Download high-res image (141KB) Download: Download full-size image
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Cell Communication and Signaling
·
2023
·Vol. 21
·期 1
·1-17
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IF 8.4
Zhao Weiming,Wang Lan,Yang Juntang,Chen Xinyu,Guo Xiaoshu,Xu Kai,Wang Ningdan,Zhao Wenyu,Xia Cong,Lian Hui,Rosas Ivan,Yu Guoying
Background Matrix metalloproteinases (MMPs) play important roles in remodeling the extracellular matrix and in the pathogenesis of idiopathic pulmonary fibrosis (IPF). MMP19, which is an MMP, was significantly upregulated in hyperplastic alveolar epithelial cells in IPF lung tissues and promoted epithelial-mesenchymal transition (EMT). Recent studies have demonstrated that endothelial-to-mesenchymal transition (E(nd)MT) contributes to pulmonary fibrosis. However, the role of MMP19 in pulmonary vascular injury and repair and E(nd)MT remains unclear. Methods To determine the role of MMP19 in E(nd)MT and pulmonary fibrosis. MMP19 expressions were determined in the lung endothelial cells of IPF patients and bleomycin (BLM)-induced mice. The roles of MMP19 in E(nd)MT and endothelial barrier permeability were studied in the MMP19 cDNA-transfected primary human pulmonary microvascular endothelial cells (HPMECs) and MMP19 adenoassociated virus (MMP19-AAV)-infected mice. The regulatory mechanism of MMP19 in pulmonary fibrosis was elucidated by blocking its interacting proteins SDF1 and ET1 with AMD3100 and Bosentan, respectively. Results In this study, we found that MMP19 expression was significantly increased in the lung endothelial cells of IPF patients and BLM-induced mice compared to the control groups. MMP19 promoted E(nd)MT and the migration and permeability of HPMECs in vitro, stimulated monocyte infiltration into the alveolus, and aggravated BLM-induced pulmonary fibrosis in vivo. SDF1 and Endothelin-1 (ET1) were physically associated with MMP19 in HPMECs and colocalized with MMP19 in endothelial cells in IPF patient lung tissues. AMD3100 and bosentan alleviated the fibrosis induced by MMP19 in the BLM mouse model. Conclusion MMP19 promoted E(nd)MT by interacting with ET1 and stimulated monocyte infiltration into lung tissues via the SDF1/CXCR4 axis, thus aggravating BLM-induced pulmonary fibrosis. Vascular integrity regulated by MMP19 could be a promising therapeutic target for suppressing pulmonary fibrosis.
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RSC Advances
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2023
·Vol. 13
·期 22
·15063-15076
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IF 3.9
Song Wang,Yonggang Zhang
Advanced oxidation processes are frequently applied to a variety of refractory organic wastewater, but rarely is electro-Fenton combined with activated persulfate technology applied to the removal of refractory pollutants. In this work, the electro-Fenton process was combined with zero-valent iron (ZVI) activated peroxymonosulfate (PMS), two advanced oxidation processes based on different radicals, to form the ZVI-E-Fenton-PMS process to treat wastewater, whose main advantages are the generation of more reactive oxygen species and the reduction of oxidant cost to achieve rapid removal of pollutants. This process can not only produce H2O2 and activate PMS at the cathode, but also reduce Fe(III) to realize the sustainable Fe(III)/Fe(II) redox cycle. The main reactive oxygen species in the ZVI-E-Fenton-PMS process were found to be ˙OH, SO4˙− and 1O2 by radical scavenging experiments and electron paramagnetic resonance (EPR), and the relative contributions of the three reactive oxygen species for the degradation of MB were estimated to 30.77%, 39.62% and 15.38%, respectively. Then, by calculating the ratio of the relative contributions of each component to the removal of pollutants at different PMS doses, it was found that the synergistic effect of the process was better when the proportion of ˙OH in the oxidation of reactive oxygen species (ROS) was higher and the proportion of non-ROS oxidation increased year-on-year. This study provides a new perspective on the combination of different advanced oxidation processes and reveals the advantages and potential of this process for application.
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APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING
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2022
·Vol. 128
·期 11
·1-11
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IF 2.983
Zhang Liang,Zhang Ran,Cui Lili,Li Tao,Gu Dayong,He Jian’an,Zhang Sixiang
The sensitization effect of graphene oxide (GO) on surface plasmon resonance (SPR) chip sensors has been widely reported. However, the absence of a fixed value for selecting the GO solution concentration is a significant limitation for reproducing the experiments reported in the literature. In this study, linear functionalized polyethylene glycol (SH-PEG-NH 2 ) is used to modify the surfaces of bare gold chips, using flow and immersion methods to produce GO-film-covered SPR sensor chips. Deionized water and phosphate buffered saline are applied as refractive index variants to compare the changes in sensor performance following chip modification, and to determine the optimal concentrations of GO solution under the respective modification methods. Using a sensor chip modified with the optimal GO concentration enhances the ability of a 10-year-old device to detect the dengue virus (DENV) E-protein by 357% and enables the detection of antibody protein concentrations as low as 62.5 ng/mL. Satisfactory results were also obtained using a DENV-positive serum to verify the chip usage. The results of this study are of significance for future efforts involving the rapid preparation of accurate and low-cost GO-SPR biosensor chips.
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WATER RESEARCH
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2022
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IF 13.4
Yi Liu,Wenhua Dong,Shuyi Shen,Fanxu Meng,Jiajia Wang,Kun Yang,Daohui Lin
Cost-effective disinfection technology is urgently needed in poor rural areas. Erythrosine (ERY)-based solar disinfection (SODIS) provides a promising solution because of its effective inactivation of viruses and gram-positive bacteria at low cost. However, the poor gram-negative bacteria (G − , e.g., Escherichia coli ) inactivation of photosensitized ERY inhibits its application. Herein, for the first time, the protonation of ERY was found to greatly enhance its G − inactivation, and 99.99999% (7.0 log) of E. coli were completely inactivated within only 30 s using 2.5 mg/L ERY under 200 mW/cm 2 visible light irradiation. The inactivation rate constant ( k ) reached 17.5 min −1 at pH 4.0, which was 4730 times higher than that at pH 7.0. At a lower pH, more severe cell wall and genomic DNA damage was observed. A linear correlation between k and monoanionic ERY (HE − ) content was obtained, indicating that HE − rather than dianionic ERY (E 2− ) participated in the inactivation at pH 5.0–7.0, which was further explained by the higher production of reactive oxygen species and bacterial adsorption of HE − than E 2− . Both 1 O 2 and O 2 − • dominated bacterial inactivation, contributing 56.8% and 43.2%, respectively. O 2 − • but not 1 O 2 caused ERY photobleaching. OH • was not involved in either inactivation or photobleaching. Humic acid and salts (NaCl, Na 2 SO 4 , CaCl 2 , and MgCl 2 ) slightly inhibited inactivation, while NaHCO 3 accelerated inactivation. Complete inactivation (99.9999%) of E. coli was achieved within ∼30 min at pH 5.0 in ERY-based SODIS with good adaptation to various water matrices and weather (sunny or partly cloudy). This work will help to promote the application of ERY-based disinfection especially for SODIS in poor rural areas.
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ACS Catalysis
·
2023
·
IF 12.9
Zhen Chen,Haixin Chen,Kun Wang,Jian Chen,Ming Li,Yi Wang,Panagiotis Tsiakaras,Shuqin Song
The photocatalytic oxygen reduction reaction (ORR) is a promising strategy that generates H2O2 with a lower energy input. Although great progress has been made in the development of photocatalysts, poor accessibility of O2 in photocatalysts interfacial microenvironment is the rate-limiting factor for the photocatalytic 2e– ORR and still little explored. Here, we design a simple method to regulate the hydrophobicity and O2 accessibility at a controllable crystal facet ratio of TiO2 photocatalyst by a facile stearic acid (SA) modification. The adsorption structure of SA bonding with TiO2 has been investigated in detail by Raman spectra, X-ray photoelectron spectroscopy, Fourier transform infrared spectra, and density functional theory calculations. It has been found that the stable ester bond is formed between SA molecules and the TiO2 surface, thus leading to the enhanced hydrophobicity with the increase of SA loading. The highest photocatalytic H2O2 production rate from 2e− ORR on SA-modified TiO2 can reach up to 3160 μM h–1 g–1, which is 1.69 times that of the corresponding pure TiO2 sample. From the results of electrochemical impedance spectroscopy and molecular dynamics simulation, the increase of the O2 concentration in the interfacial microenvironment benefiting from the SA modification is responsible for the enhancement of 2e– ORR activity rather than the increase of hydrophobicity.
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JOURNAL OF HAZARDOUS MATERIALS
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2021
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IF 10.588
Xiaoman Xie,Xirui Tan,Yiyan Yu,Yunchong Li,Pengbo Wang,Yuanhao Liang,Yunjun Yan
Conventional mining processes of rare earth elements (REEs) usually produce REEs-rich industrial waterwastes, which leads to a significant waste of REEs resources and causes serious environmental pollution. Biosorption using engineered microorganisms is an attractive technology for the recovery of REEs from aqueous solution. To regulate the REEs’ adsorption and recovery by sensing extraneous REEs, an engineered cascaded induction system , pmrCAB operon containing a lanthanide-binding tag (LBT) for sensing REEs, was incorporated into E. coli in conjunction with a silica-binding protein (Si-tag) and dLBT anchored onto the cell membrane. The sensing and adsorption capacities for Terbium (Tb), a typical study subject of REEs, were enhanced by screening an effective LBT and increasing the dLBT copy number. The adsorption capacity for Tb reached the highest reported value of 41.9 mg g −1 dry cell weight (DCW). After adhering the engineered cells onto the silica column surface through overexpressed Si-tag, a high recovering efficiency (> 90%) of Tb desorption could be obtained with 3 bed volumes of citrate solution. In addition, the engineered cells also possessed fairly good adsorption capacity of other tested REEs. Our findings showed that the recovery of REEs with high efficiency, selectivity and controllability from aqueous solution can be well achieved via specifically bio-engineered strains.
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FOOD AND CHEMICAL TOXICOLOGY
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2023
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IF 4.3
Kaiyuan Chen,Yahui Tang,Linhua Lan,Mengxuan Li,Zhongqiu Lu
Acute kidney injury (AKI) induced by diquat (DQ) progresses rapidly, leading to high mortality, and there is no specific antidote for this chemical. Our limited knowledge of the pathogenic toxicological mechanisms of DQ has hindered the development of treatments against DQ poisoning. Pyroptosis is a form of programmed cell death and was recently identified as a novel molecular mechanism of drug-induced AKI. To explore the role of pyroptosis in HK-2 cells exposed to DQ, the plasma membrane damage of the cells was detected by LDH release assay. Western blot was performed to detect the cleavage of GSDME. Proteomics analysis was performed to explore the mechanism of DQ induced nephrotoxicity . FerroOrange probe was used to measure the intracellular Fe 2+ levels. Herein, we show that DQ induces pyroptosis in HK-2 cells. Mechanistically, DQ induces the accumulation of mitochondrial ROS and initiates the cleavage of gasdermin E (GSDME) in an intrinsic mitochondrial pathway. Knockout of GSDME attenuated DQ-induced cell death. Further analysis revealed that loss of FTH1 induces Fe 2+ accumulation, contributing to DQ-induced pyroptosis. Knockdown LC3B could help restore the expression of FTH1 and improve cell viability . Moreover, we found DFO , an iron chelator , could reduce cellular Fe 2+ levels and inhibit pyroptosis. Collectively, these findings suggest an unrecognized mechanism for GSDME-dependent pyroptosis in DQ-induced AKI.
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Nanomaterials
·
2023
·Vol. 13
·期 22
·2954
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IF 5.3
Suyi Zhao,Zhenzeng Chong,Xiaogang Zuo,Wenjun Qi
For environmental remediation, it is significant to design membranes with good mechanical properties and excellent photocatalytic activity. In this work, RGO/TiO2membranes with heterogeneous structures and good photocatalytic efficiency were synthesized using the method of electrospinning combined with a thermal treatment process. In the binary nanocomposites, RGO was tightly adhered to TiO2fibers and by simply adjusting the loading of RGO, the strength and modulus of the fibrous membranes were improved. Notably, the RGO-permeated TiO2fibers exhibited 1.41 MPa in tensile strength and 140.02 MPa in Young’s modulus, which were 705% and 343% of the original TiO2fibers, respectively. Benefiting from the enhanced light response and the homogeneous and compact heterogeneous structure, the synthesized RGO/TiO2membranes displayed good antibacterial performance with a photocatalytic inactivation rate of 6 log againstE. coliwithin 60 min. This study offers a highly efficient alternative to inactivateE. colifor the synthesis of TiO2-based membranes.Keywords:RGO/TiO2;nanomembrane;photocatalytic;sterilization
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CARBON
·
2023
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IF 10.9
Xuewei Shi,Alamusi Lee,Bo Yang,Lingxiao Gao,Huiming Ning,Kaiyan Huang,Xiaolin Luo,Lidan Zhang,Jiaofei Zhang,Chao Yang,Bin Gu,Ning Hu
Artificial hydrogel electronic skin (E-skin) sensing systems have received considerable attention due to their ability in providing tactile perception. Recent advancements in hydrogel E-skin have allowed for simultaneous sensing of touch position and pressure through construction of sensor arrays. However, the fabrication process is complex, and the sensing resolution is limited by the size and number of array cells. Here, we report a multi-modal sensory E-skin based on a polyvinyl alcohol (PVA) hydrogel embedded with carbon nano-materials, which can recognize touch position and pressure simultaneously without constructing arrays. Combining the advantages of high electron transfer efficiency of 1D nanotube and 2D graphene nanosheet, the 3D hierarchical cross-linked polydopamine (PDA) modified reduced graphene oxide (rGO)/carboxylic multiwalled carbon nanotube (MWCNT-COOH) composites were proposed as a conductive filler, which was added by nano-doping to hydrogel substrate to gain an elastic modulus and stretchability of the hydrogel similar to that of human skin. Meanwhile, a surface-capacitive touch (SCT) system and piezoresistive technology were adopted to sense touch position and pressure due to the stability of conductive networks and the piezoresistive properties of carbon nanomaterials. Our E-skin device demonstrates superior sensing performance and provides a new direction for designing novel E-skin sensor devices.
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