ELECTROPHORESIS
·
2018
·Vol. 39
·期 22
·2912-2918
·
IF 2.569
Deying Kong,Zilin Chen
Because of unique properties like permanent porosity, low density and large surface area, covalent organic frameworks are attractive microporous materials as stationary phase for CEC. Among them, COF-TpPa-1 possesses extraordinary chemical stability and common features of covalent organic frameworks. So, COF-TpPa-1 could be a promising material as stationary phase to enhance separation selectivity and tolerate the acid or alkaline electrochromatographic separation conditions. Here, we report the preparation of COF-TpPa-1 modified capillary column by in situ growth for electrochromatographic separation. The immobilization of COF-TpPa-1 on the inner wall of capillary column was confirmed by Fourier transform infrared spectroscopy, scanning electron microscopy and X-ray diffraction. The fabricated COF-TpPa-1 modified capillary column indicated good resolution for the separation of neutral analytes, nonsteroidal anti-inflammatory drugs and food additives in open-tubular CEC mode. Compared with bare capillary column, the COF-TpPa-1 modified capillary column improved separation selectivity remarkably for tested analytes, including hydrophobic, π–π and hydrogen bond interactions. Besides, the prepared capillary column showed excellent repeatability and stability. The intraday, interday and column-to-column relative standard deviations of migration time for neutral analytes were less than 3.54%. This work shows enamine-linked covalent organic frameworks have great potential as stationary phases in CEC.
研究领域:
分析化学
·
材料科学
✓ 已验证
DOI
高影响因子
RSC Advances
·
2014
·Vol. 4
·期 52
·27259-27266
·
IF 3.708
Libin Yang,Feng Xin,Junzhong Lin,Zhuang Zhuang,Rui Sun
In this study, a hydrothermal synthesis was developed to prepare a thin titanium silicalite-1 catalyst layer in the internal channels of the honeycomb cordierite support, which was used in a continuous heterogeneous reaction. The as-prepared thin TS-1 catalyst layer was characterized by SEM, XRD, UV-vis and ATR-FTIR methods. Multiple hydrothermal syntheses were used for controlling the crystalline growth to optimize catalytic activity and lifetime. Moreover, a monolithic TS-1/cordierite reactor with external looping was utilized to investigate the characteristics of the continuous heterogeneous ammoximation reaction without the organic solvent tert-butanol (TBA). The reaction results showed that the conversion of cyclohexanone and selectivity of oxime approached more than 0.96 and 0.98, respectively. When compared with the reaction using TBA as the reaction solvent, the activity of the monolithic TS-1/cordierite showed no differences except for a longer lifetime.
研究领域:
化学工程
·
催化化学
·
材料科学
·
工业化学
·
绿色化学
✓ 已验证
DOI
高影响因子
JOURNAL OF SOLID STATE CHEMISTRY
·
2025
·
IF 3.5
Huanxu Teng,Meihua Zhu,Yixuan Shao,Siqi Shao,Qingsheng Wu,Ziming Li,Xiangshu Chen,Hidetoshi Kita
Supported TS-1 zeolites were successfully synthesized on α-Al 2 O 3 microsphere by secondary hydrothermal synthesis, which were placed in the fixed-bed reactor and catalyzed the oxidation of isopropanol(IPA) with hydrogen peroxide. Effects of synthesis parameters on the morphology and catalytic activity of the supported TS-1 zeolites were systematically studied in this work. Size of α-Al 2 O 3 microsphere, and crystallization temperature could determine the coverage, titanium coordination, and titanium content of the supported TS-1 zeolites. When the crystallization temperature and H 2 O/SiO 2 molar ratio of the initial synthesis solution were 150 °C and 120, the titanium-rich and anatase-free supported TS-1 zeolites were prepared on the ca. 1 mm α-Al 2 O 3 microsphere support. The supported TS-1 zeolites showed an excellent catalytic performance and stability for isopropanol oxidation, the isopropanol conversion and acetone selectivity reached 100 % within 2 h. Kinetic studies showed that the reaction conformed to the Langmuir-Hinshelwood mechanism and that the apparent activation energy was only 4.57 kJ/mol.
研究领域:
催化化学
·
材料化学
·
化学工程
·
多相催化
·
环境化学
✓ 已验证
产品关联
DOI
高影响因子
Theranostics
·
2021
·
IF 11.556
Shi-Long Zhang,Yu-Qin Mao,Zheng-Yan Zhang,Zhan-Ming Li,Chao-Yue Kong,Hui-Ling Chen,Pei-Ran Cai,Bing Han,Tao Ye,Li-Shun Wang
Background: Anti-PD-1-based immunotherapy has emerged as a promising therapy for several cancers. However, it only benefits a small subset of colorectal cancer (CRC) patients. Mounting data supports the pivotal role of gut microbiota in shaping immune system. Pectin, a widely consumed soluble fiber, has been reported to ameliorate the imbalance of gut microbiota. Therefore, we aimed to explore the effect and the underlying mechanisms of pectin in improving anti-PD-1 mAb efficacy. Methods: The C57BL/6 mice were treated with a broad-spectrum antibiotic (ATB) cocktail to depleted endogenous gut microbiota and subsequently humanized with feces from healthy controls or newly diagnosed CRC patients. The antitumor efficacies of anti-PD-1 mAb combined with or without pectin were assessed using these mice. Flow cytometry and immunohistochemistry (IHC) were conducted to investigate the tumor immune microenvironment after treatment. The gut microbiota profiles and short-chain fatty acids (SCFAs) levels were determined by 16S ribosomal RNA (16S rRNA) gene sequencing and gas chromatography-mass spectrometry (GC-MS), respectively. The effect of gut microbiota on anti-PD-1 mAb efficacy after pectin supplement was further tested by fecal microbiota transplantation (FMT). Results: The anti-PD-1 mAb efficacy was largely impaired in the mice humanized with feces from newly diagnosed CRC patients compared to those from healthy controls. However, pectin significantly enhanced the anti-PD-1 mAb efficacy in the tumor-bearing mice humanized with CRC patient gut microbiota. Flow cytometry and IHC analysis revealed increased T cell infiltration and activation in the tumor microenvironment of mice treated with anti-PD-1 mAb plus pectin. In vivo depletion of CD8 + T cells diminished the anti-tumor effect of anti-PD-1 mAb combined with pectin. 16S rRNA gene sequencing showed that pectin significantly increased gut microbial diversity and beneficially regulated microbial composition. In addition, we identified unique bacterial modules that were significantly enriched in the anti-PD-1 mAb + pectin group, which composed of butyrate-producing bacteria indicative of good response to immunotherapy. Meanwhile, GC-MS showed that pectin altered the level of SCFA butyrate. Furthermore, butyrate, a main product of dietary fiber in gut microbial fermentation, was found to be sufficient to promote T cells infiltration and thus enhance the efficacy of anti-PD-1 mAb. In addition, FMT demonstrated the effects of pectin were dependent on gut microbiota. Importantly, the beneficial effects of pectin were confirmed in the mice humanized with gut microbiota from patient with resistance to anti-PD-1 mAb. Conclusion: Pectin facilitated the anti-PD-1 mAb efficacy in CRC via regulating the T cell infiltration in the tumor microenvironment, which was potentially mediated by the metabolite butyrate.
研究领域:
肿瘤学
·
免疫学
·
微生物学
✓ 已验证
DOI
PubMed
高影响因子
Catalysts
·
2022
·Vol. 12
·期 12
·1625
·
IF 4.501
Bo Chen,Zhi-Ze Cao,Zhi-Jun Diao,Liang-Qiu Huang,Si-Jia Zhao,Hong Yuan,Jia-Meng He
Hydrogenolysis via targeted depolymerization of C–O linkages is a techno-economic beneficial process for converting lignin into highly valuable chemicals and clean fuels. In this work, a macroporous silicalite-1 (S-1) array-supported Ru-Ni metallic phosphide composite (Ru-Ni12P5/S-15) was prepared as a catalyst and hydrogenolysis activity under relative mild conditions was investigated using a series of compounds containing ether linkages as lignin-related model compounds. The Lewis acid sites originating from the unreduced Ru species and the macroporous geometry of S-1 significantly influenced hydrogenolysis activity and product selectivity. Analysis of the mechanism demonstrated that both the aryl ether and aliphatic ether linkages were directly hydrogenated over Ru-Ni12P5/S-15. 2D-HSQC-NMR spectroscopy demonstrated that the ether linkages of lignin were efficiently cleaved by Ru-Ni12P5/S-15. Furthermore, the obtained liquid hydrogenolysis products are high value-added chemicals used for pharmaceutical production and can be facilely tuned via the reaction conditions.
研究领域:
催化化学
·
可再生资源转化
✓ 已验证
DOI
高影响因子
KOREAN JOURNAL OF CHEMICAL ENGINEERING
·
2012
·Vol. 29
·期 10
·1388-1392
·
IF 0.991
He Minqiang,Pan Aixia,Xie Jimin,Li Huamin,Yuan Xinhua,Cheng Xiaonong,Chen Min
A novel one-pot catalytic synthesis of 1-benzoylpyrene through acylation of pyrene with benzoic anhydride catalyzed by several heteropoly acids (HPAs) was investigated. Pure 1-benzoylpyrene was obtained and its structure was identified by GC/MS, FT-IR and 1 H NMR spectra. Silica-supported phosphotungstic heteropoly acid (PW/SiO 2 ) was found to be the most active catalyst in the acylation. The yield and the selectivity of 1-benzoylpyrene were up to 62.5% and 100%, respectively. The effects of experimental parameters on the catalytic acylation reaction, and the possibility of reusability of PW/SiO 2 catalyst were studied. PW/SiO 2 catalyst is easily separable from the reaction mixture and reusable without loss of its activity.
研究领域:
有机化学
·
催化材料科学
✓ 已验证
DOI
高影响因子
Science of Advanced Materials
·
2016
·Vol. 8
·期 5
·1145-1153
·
IF 1.812
Ma Wang,Yi Shengen,Lin Liangwu,Huang He,Peng Tao,Liu Zhongtao,Liu Zhipeng,Pei Dongni,Miao Xiongying,Wen Yu,Chen Wei,Xiong Li
To synthesize a novel photosensitizer loaded into hollow titanium dioxide nanoparticles (HTNP) and evaluate the efficacy of its anticancer effects on Panc-1 pancreatic cancer cells in vitro. DLS measurements showed that the size of the particles ranged from 80 to 1,000 nm, with
an average diameter of 120 nm. The PDI of the Photosan-loaded HTNP was 0.112 ± 0.01, indicating that the samples have excellent quality with a narrow size distribution in monomodal systems. The photodynamic effects were positively related to the photosensitizer concentration, the light
dose, and the incubation duration. Using the same parameters, photodynamic therapy induced with Photosan-loaded HTNP had a more severe inhibition effect on cancer cell growth than that induced using free Photosan. Moreover, the nano-Photosan groups produced effects within a shorter duration
than free Photosan. Neither the Photosan-loaded nanoparticles nor free Photosan affected H6C7 cells cultured from a normal pancreas. The flow cytometry results demonstrated that laser-induced cell death with Photosan-loaded HTNP was substantially more severe than that induced with free Photosan.
Photosan-loaded HTNP are safe and stable and exhibit faster and more effective tumoricidal effects than free Photosan in the inhibition of Panc-1 cell growth.
研究领域:
肿瘤学
·
纳米医学
·
细胞生物学
✓ 已验证
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2021
·
IF 7.312
Shuang Qiu,Jinghua Du,Yonghou Xiao,Qidong Zhao,Gaohong He
Ultra-deep removal of thiophene sulfides from transportation fuel via adsorption has aroused great attention under the pressure of gradually demanding environmental regulations. The development of high-efficiency adsorbents is the key to adsorptive desulfurization for producing clean transportation fuels. In this work, a microwave-assisted synthesis method was applied to rapidly fabricate hierarchical porous defective HKUST-1 (DEHKUST-1) towards removing benzothiophene from model fuel by selective adsorption. The effects of cetyltrimethylammonium bromide (CTAB) dosage, adsorption temperature and adsorption contact time on the DEHKUST-1 desulfurization performance were systematically investigated. The characterization results proved that both metal clusters and linker defects were generated in the as-prepared DEHKUST-1. CTAB as template could modulate the pore distribution and introduce reasonable defects in the framework of HKUST-1. At CTAB: Cu 2+ = 0.5 by mole ratio, a regular mesoporous distribution of 25–30 nm could be obtained with DEHKUST-1; it exhibited an impressive enhancement in adsorptive desulphurization performance. The maximum adsorption capacity of the adsorbent attained 14.4 mg S/g adsorbent at 20 °C in 4 h. Even after CTAB-H-0.5 was regenerated six times, its adsorption capacity remained 93% as that of the as-prepared adsorbent. An adsorption mechanism was proposed to illustrate the promoting desulfurization performance by introduction of hierarchical porous in HKUST-1. By virtue of the rapid synthesis and remarkable benzothiophene removal capacity, the DEHKUST-1 could be a potential adsorbent in desulfurization from real fuels.
研究领域:
化学工程
·
材料科学
·
环境科学
✓ 已验证
DOI
高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2013
·Vol. 97
·期 24
·10423-10433
·
IF 3.689
Yang Xueqing,Zhang Yalin
Carboxylesterases (CEs) are enzymes responsible for the detoxification of insecticides in insects. In the Cydia pomonella , CEs are involved in synthetic pyrethroid, neonicotinoid, carbamate, and organophosphate detoxification. However, functional overexpression of CEs proteins in Escherichia coli systems often results in insoluble proteins. In this study, we expressed the fusion protein CpCE-1 in E . coli BL21 (DE3). This recombinant protein was overexpressed as inclusion bodies at 37 °C whereas it produced a higher percentage of soluble protein at lower growth temperatures. Production of soluble proteins and enzyme activity increased in the presence of sorbitol in the growth medium. The fusion protein was purified from the lysate supernatant using a Ni 2+ -NTA agarose gel column. The enzyme exhibited a higher affinity and substrate specificity for α-naphthyl acetate (α-NA), with k
cat / K
m of 100 s −1 μM −1 for α-NA, and the value is 29.78 s −1 μM −1 for β-naphthyl acetate. The V
max and K
m were also determined to be 12.9 μmol/min/mg protein and 13.4 μM using substrate α-NA. The optimum pH was 7.0 and temperature was 25 °C. An enzyme inhibition assay shows that PMSF and DEPC strongly inhibit the enzyme activity, while the metal ions Cu 2+ and Mg 2+ significantly activated the activity. More importantly, cypermethrin, methomyl, and acephate were found to suppress enzyme activity. The data demonstrated here provide information for heterologous expression of soluble protein and further study on insecticide metabolism in C . pomonella in vitro. This is the first report of the characterization of CEs protein from C . pomonella .
研究领域:
昆虫学
·
生物化学
·
农药代谢
✓ 已验证
DOI
PubMed
高影响因子
ONCOLOGY REPORTS
·
2024
·Vol. 52
·期 4
·1-8
·
IF 3.8
Tong Wang,Xu Ye,Hao Jiang,Yu Gao
B‑cell lymphoma is difficult to cure because of its biological and clinical heterogeneity, and due to native chemoresistance. Immunotherapies that overcome cancer‑induced immune evasion have been the center of recent developments in oncology. This is emphasized by the accomplishment of various agents that disrupt programmed cell death protein 1 (PD‑1)‑mediated immune suppression in diverse tumors. However, while PD‑1 blockade has been effective in numerous malignancies, a significant proportion of cancers, including B‑cell lymphoma, show certain rates of primary resistance to these therapeutic strategies. Histone deacetylase inhibitors (HDACis) have exhibited anticancer activity though suppressing cell proliferation, inducing differentiation and triggering apoptosis. The present study aimed to explore a therapeutic strategy combining a HDACi (romidepsin) and PD‑1 blockade (BMS‑1) in B‑cell lymphoma, utilizing a constructed mouse model of B‑cell lymphoma. The IC50 of the two inhibitors was confirmed by MTT assay, and their inhibitory effects were revealed to be dose‑ and time‑dependent. The data demonstrated that the combined treatment of romidepsin and BMS‑1 synergistically inhibited the growth of B‑cell lymphoma. Furthermore, it was revealed that romidepsin and BMS‑1 synergistically triggered apoptosis in mouse B‑cell lymphoma. The synergistic effect of these agents was capable of activating tumor‑infiltrating lymphocytes, particularly CD3+CD4+ and CD3+CD8+ T cells. The results of the present study underscore the potential of HDAC inhibition in conjunction with PD‑1 blockade as a novel therapeutic approach for B‑cell lymphoma, highlighting the synergistic effects of these two mechanisms in enhancing antitumor immunity.
研究领域:
肿瘤学
·
免疫学
·
分子生物学
·
癌症治疗
✓ 已验证
DOI
PubMed
高影响因子
ENERGY & FUELS
·
2019
·
IF 3.021
Yuhao Zhang,Haoran Gao,Mengxuan Wang,Liang Zhao,Jinsen Gao,Chunming Xu
Recent studies have investigated the separation of olefins from fluid catalytic cracking naphtha by solvent treatment to produce motor gasoline. This study explored the effect of the structure and group of six solvents on the separation of the 1-hexene–benzene and 1-hexene–thiophene systems. Solvents with a chain structure, such as N-methyl diethanolamine (MDEA) and tetraethylene glycol, showed satisfactory separation performance for olefins but poor performance for benzene and thiophene. This result can be ascribed to the weak polarity of the chain structure for the low solubility of benzene and thiophene. By contrast, solvents with a ring structure separated 1-hexene by enhancing the solubility of the mixture for the solvent, especially the dissolution of benzene and thiophene. The selective dissolution of benzene and thiophene by the solvent containing a ring structure was generally greater than that by the solvent containing a chain structure. At the highest 1-hexene content, the solubility of N-methyl diethanolamine for benzene and thiophene was higher than 11, whereas that of MDEA with the chain structure was only 7. The highly electronegative sulfoxide group improved the selectivity for olefins. The selectivity θ of 1-hexene by sulfolane and dimethyl sulfoxide in separating 1-hexene from benzene or thiophene was about 2–14 times that of alcohol amine solvents. Therefore, the ring structure primarily enhanced the processing capacity of the solvents in the separation of the olefins–aromatics/sulfide system, whereas the sulfoxide group improved the selectivity of the solvents in the separation of olefins.
研究领域:
化学工程
·
能源科学
✓ 已验证
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2017
·
IF 7.504
Xiang Cai,Tao Yuan,Xiangfu Liu,Guoli Tu
Solid alcohol 1-pyrenemethanol (PyM) was first introduced to modify the zinc oxide (ZnO) layer which is used in the inverted polymer solar cells (PSCs) as a cathode buffer layer (CBL). As a low-cost industrial product, the PyM can modify the surface defects and improve the electron mobility of ZnO CBL, which can be attributed to the self-assembly of PyM on the ZnO surface due to the hydrogen bonds and the conjugated structure in PyM. With a blend of PTB7:PC71BM as active layer, the device with ZnO/PyM CBL exhibited a notable power conversion efficiency (PCE) of 8.26%, which is better than that of control devices based on bare ZnO CBL (7.26%). With the addition of PyM, the device based on PTB7-Th:PC71BM showed a higher PCE of 9.10%, an obvious improvement from the 7.79% of control devices. There was no obvious change in device performance with the increase of PyM solution concentration, indicating that the device fabrications are thickness-insensitive. These results could be particularly useful in solution processing of buffer layer materials to high-efficiency organic solar cells.
研究领域:
材料科学
·
能源科学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）