JOURNAL OF MEDICAL VIROLOGY
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2024
·Vol. 96
·期 6
·e29723
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IF 6.8
Dekai Zheng,Changhao Cheng,Yanhua Tang,Zhixin Fang,Xuelian Gao,Yuchuan Chen,Qiuhong You,Kaifeng Wang,Heqi Zhou,Zhixian Lan,Jian Sun
Hepatitis B virus (HBV) can be completely suppressed after antiviral treatment; however, some patients with chronic hepatitis B (CHB) exhibit elevated alanine aminotransferase (ALT) levels and sustained disease progression. This study provides novel insights into the mechanism and potential predictive biomarkers of persistently elevated ALT (PeALT) in patients with CHB after complete viral inhibition. Patients having CHB with undetectable HBV DNA at least 12 months after antiviral treatment were enrolled from a prospective, observational cohort. Patients with PeALT and persistently normal ALT (PnALT) were matched 1:1 using propensity score matching. Correlations between plasma metabolites and the risk of elevated ALT were examined using multivariate logistic regression. A mouse model of carbon tetrachloride-induced liver injury was established to validate the effect of key differential metabolites on liver injury. Of the 1238 patients with CHB who achieved complete viral suppression, 40 (3.23%) had PeALT levels during follow-up (median follow-up: 2.42 years). Additionally, 40 patients with PnALT levels were matched as controls. Ser-Phe-Ala, Lys-Ala-Leu-Glu, 3-methylhippuric acid, 3-methylxanthine, and 7-methylxanthine were identified as critical differential metabolites between the two groups and independently associated with PeALT risk. Ser-Phe-Ala and Lys-Ala-Leu-Glu levels could be used to discriminate patients with PeALT from those with PnALT. Furthermore, N -acetyl- l -methionine (NALM) demonstrated the strongest negative correlation with ALT levels. NALM supplementation alleviated liver injury and hepatic necrosis induced by carbon tetrachloride in mice. Changes in circulating metabolites may contribute to PeALT levels in patients with CHB who have achieved complete viral suppression after antiviral treatment.
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
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2025
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IF 3.9
Jian Wei,Yucui Hou,Tianqi Huang,Aohua Zheng,Shuhang Ren,Weize Wu
Identifying the source of products in lignin hydro-liquefaction oil is crucial for assessing the lignin conversion efficiency. The utilization of hydrogen donor solvents (HDSs) complicates the determination of whether polycyclic aromatic hydrocarbons (PAHs) derive from lignin or HDS. Thus, identifying the source of PAHs in the oil can improve our understanding of lignin structures and enhance its utilization. To address this challenge, 1,2,3,4-tetrahydroquinoline (THQ) was used as an HDS to trace products within the oil. The results showed that (1) lignin could be completely liquefied in THQ under optimal conditions: a THQ/lignin mass ratio of 0.5, a reaction temperature of 450 °C, an initial H2 pressure of 8 MPa, a reaction time of 4 h, and a molybdenum sulfide/lignin mass ratio of 1; (2) PAHs with 2–6 rings were identified in the oil and confirmed to derive solely from lignin through qualitative and quantitative analyses via GC–MS rather than THQ.
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Tissue Engineering Part A
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2014
·
IF 0
Shane Gao,Peng Zhao,Chao Lin,Yuxi Sun,Yilei Wang,Zhichong Zhou,Danjing Yang,Xianli Wang,Hongzhen Xu,Fei Zhou,Limei Cao,Wei Zhou,Ke Ning,Xu Chen,Jun Xu
Multipotent human adipose-derived stromal/stem cells (hADSCs) hold a great promise for cell-based therapy for many devastating human diseases, such as spinal cord injury and stroke. If exogenous hADSCs can be cultured in a three-dimensional (3D) scaffold with effective proliferation and differentiation capacity, it will better mimic the in vivo environment, which will have profound impact on the therapeutic application of hADSCs. In this study, a group of elastic-dominant, porous bioscaffolds from photocurable chitosan and gelatin were fabricated and proven to be biocompatible with both hADSCs and hADSC-derived neuron-like cells (hADSC-NLCs) in vitro. The identity of harvested hADSCs was confirmed by their positive immunostaining of mesenchymal stem cell surface markers, CD29, CD44, and CD105, and also positive expression of stem markers, Sox-2, Oct-4, c-Myc, Nanog, and Klf4. Their multipotency was further confirmed by trilineage differentiation of hADSCs toward adipocyte, osteoblast, and chondrocyte. It was found that hADSCs could be conditioned to differentiate into neurons in vitro as determined by immunostaining the markers of Tuj1, MAP2, NeuN, and Synapsin. The hADSCs and hADSC-NLCs were proven to be biocompatible with 3D scaffold, which actually facilitated the proliferation and differentiation of hADSCs in vitro, by MTT assay and their neuronal gene expression profiling. Moreover, hADSC-NLCs, which were mixed with 3D scaffold and transplanted into traumatic brain injury mouse model, survived in vivo and led to the better repair of the damaged brain area. The immunohistochemical studies revealed that 3D scaffold indeed improved the viability of transplanted cells, their ability to incorporate into the in vivo neural circuit, and their capacity for tissue repair. This study indicates that hADSCs would have great therapeutic application potential as seeding cells for in vivo transplantation to treat various neurological diseases when co-applied with porous chitosan/gelatin bioscaffolds.
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OPTICAL MATERIALS
·
2024
·
IF 3.8
Zifang Xu,Yong Fan,Yan Dai,Xuekai Wang,Shuangyue Hu
A fast response and stability enhanced electrochromic film based on a composite of polyaniline (PANI) with surface functionalized Lanthanum oxide (f-La 2 O 3 ) nanoparticles has been prepared by in-situ polymerization. Scanning electron microscopy (SEM) and Fourier infrared spectroscopy (FTIR) confirmed that La 2 O 3 was attached to the surface of PANI by silane coupling agent. Meanwhile, Electrochemical and electrochromic properties were investigated using cyclic volatammetry, chronoamperommetry, electrochemical impedance spectroscopy (EIS) and UV–vis spectroscopy. Compared with PANI, the f-La 2 O 3 /PANI composite showed excellent electrochromic performance, shorter response time and higher stability. As the mol ratio of La 2 O 3 to aniline was 1:3.5, the composites exhibited best electrochromic behavior with superior coloration efficiency of 22.81 cm 2 C −1 and fastest response time of 1.29 s/1.33 s for bleaching and coloration respectively. The result indicates that introduction of rare earth elements has great potential to improve the electrochromic properties of conducting polymers .
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STEROIDS
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2011
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IF 3.106
Long Ji,Yonglei Yuan,Liping Luo,Zhe Chen,Xiaoqiong Ma,Zhongjun Ma,Lin Cheng
Michael reaction acceptors (MRAs) are a class of active molecules that are directly or indirectly involved in various cellular processes, including the regulation of many signaling pathways . In this study, the inducible nitric oxide synthase (iNOS) assay was used to demonstrate that the dichloromethane extract of Physalis alkekengi var . franchetii (DCEP) possesses anti-inflammatory activity that might be attributed to the modification of key cysteine residues in IKKβ by the MRAs in DCEP. To isolate these MRAs, glutathione (GSH) was employed, and a simple ultra-performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) screening method was developed to investigate the GSH conjugates with potential MRAs. Five physalins, including one new compound isophysalin A ( 2 ), together with four known steroidal compounds, physalin A ( 1 ), physalin O ( 3 ), physalin L ( 4 ) and physalin G ( 5 ), were isolated to evaluate the GSH conjugating abilities, and it was indicated that compounds 1 , 2 and 3 , which had a common α,β-unsaturated ketone moiety, exhibited conjugating abilities with GSH and also showed significant nitric oxide (NO) production inhibiting activities. The anti-inflammatory activities of compounds 1 , 2 and 3 might be attributed to their targeting multiple cysteine residues on IKKβ; therefore, the alkylation of IKKβ by compound 1 was further studied by micrOTOF-MS. The result showed that six cysteine residues (C 59 , C 179 , C 299 , C 370 , C 412 , and C 618 ) were alkylated, which indicated that IKKβ is a potential target for the anti-inflammatory activity of physalin A.
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INDUSTRIAL CROPS AND PRODUCTS
·
2025
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IF 6.2
Zhiqiang Shan,Xiaohua Jia,Jin Yang,Zhaofeng Wang,Haojie Song
The waste products of agroforestry production and processing (stalk, straw, hulls, wood flour) are often considered to be worthless materials and are usually buried or burned. A few valuable by-products can be further utilized and used for chemical composting or handicrafts. Herein, the deep eutectic solvent (DES) defibrillation and chlorine-acid oxidation processes were used to crack and remove lignin of waste poplar powder to obtain esterified nanocellulose (NC). A further ice-template method was used to construct 2D woven NC layers with transversely large-sized structures. The ZIF-8 nanoparticles and polydopamine (PDA) coatings were sequentially grown on the NC layer surface to obtain NC@ZIF-8/PDA hybrid composites. The ZIF-8 nanoparticles have high negative amount on NC layer surface, and the PDA coatings enhanced the mechanical stability of the hybrid NC@ZIF-8/PDA and prevented the degradation of ZIF-8 in water condition. Importantly, NC@ZIF-8/PDA was introduced into PVA matrix to obtain NZPP films which shows the high tensile strength (101.58 MPa), excellent elongation at break (202.8 %) and toughness (152.52 MJ/m 3 ), high impact strength (68.9 KJ/m 2 ), and self-lubricating capability. The highly woven and entangled NC layers and ZIF-8 with internal pore structure produces fine structural changes and spatial constraints under applied loads and shear stresses, which leads to irregular deformation of the friction contact surface, thus accelerating the distribution of friction stresses and friction energy transfer.
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CONSTRUCTION AND BUILDING MATERIALS
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2024
·
IF 7.4
Yuming Wang,Xiaomei Wang,Fang Yao,Chang Lin,ShuYing Xu,Yan Li,Juan Du,Lisha Pan
This study focuses on developing three kinds of polycarboxylate superplasticizers (PCEs), i.e., ether-type polycarboxylate superplasticizer (T-PCE), fluorine-type polycarboxylate superplasticizer (F-PCE), and phosphate-type polycarboxylate superplasticizer (P-PCE) synthesized respectively with three functional monomers, i.e., isopentenyl polyoxyethylene ether (TPEG3000), hexafluorobutyl methacrylate (HFBMA), and 2-hydroxyethyl methacrylate phosphate (HEMAP) for improved dispersion and low air entrainment in cementitious systems. The molecular structures of PCEs were characterized by Fourier Transform Infrared Spectroscopy (FTIR), Gel Permeation Chromatography (GPC), and Proton Nuclear Magnetic Resonance ( 1 H NMR). Further, the influence and mechanism of the functional monomers on the dispersion and air entrainment of PCEs in cement paste and mortar were investigated. Finally, the mechanisms of the functional monomers' effect on the air entrainment of PCEs in the cement paste and mortar were discussed. The results showed that T-PCE, F-PCE, and P-PCE were successfully synthesized, and P-PCE had the optimal effect on the fluidity (at W/C=0.35, 305.5 mm), the lowest foaming height (11.000 mm), and the fastest rate of defoaming of the cementitious systems, followed by F-PCE and T-PCE. Complexation, adsorption, surface activity, and polar hydrophobic mechanisms were identified. For unmodified T-PCE, there are more hydrophilic groups (-COOH), stronger surface activity. Therefore, T-PCE shows strong air entrainment; T-PCE has a high density of carboxylate and often forms a complex with 1 Ca 2+ in the form of 4 carboxyl groups before reaching the adsorption equilibrium, so the concentration of free Ca 2+ with foam stabilizing effect is high, which leads to strong air entrainment of T-PCE. The adsorption and complexation ability of F-PCE with Ca 2+ is weaker than that of T-PCE. However, the hydrophobicity of F-PCE molecules is enhanced and the surface tension of F-PCE is reduced due to the ester group and C-F bonds in the structure of HFBMA, so the air entrainment of F-PCE is lower than that of T-PCE through Marangoni effect. P-PCE exhibited the lowest surface activity and the largest surface tension. Defoaming effect is promoted due to increased additional pressure on the bubble surface. Moreover, its adsorption and complexation with Ca 2+ surpassed those of T-PCE and F-PCE due to the phosphate group has two negative charges, and the free Ca 2+ is reduced, which also reduces the bubble stability. Therefore, P-PCE has minimal air entrainment in the cementitious systems.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2018
·
IF 3.412
Yao Pan,Ze-yuan Deng,Shi-lian Zheng,Xuan Chen,Bing Zhang,Hongyan Li
The hydrophilic extracts of mulberry (HEM) and blueberry (HEB) and lipophilic extracts of mango (LEM) and watermelon (LEW) were mixed in different ratios to assess the antioxidant interactions by chemical-based (DPPH and ABTS assays) and H9c2 cell-based models. There were both synergistic and antagonistic antioxidant interactions among these fruits. Some groups with combinational extracts showed stronger synergistic antioxidant effects than the individual groups, and others (HEM–LEW F1/10, LEW–LEM F5/10, and HEB–LEM F3/10) showed stronger antagonistic effects than the individual groups based on the indicators [the values of DPPH, ABTS, and MTT; the expression of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT), and malondialdehyde (MDA); the release of lactate dehydrogenase (LDH); and the quantification of cellular antioxidant activity]. The principal component analysis (PCA) showed that samples could be defined by two principal components: PC1, the main phenolic acids and anthocyanins, and PC2, carotenoids. From our results, primarily, carotenoids were in the majority in antagonistic groups, and phenolics and anthocyanins were in the majority in synergistic groups. However, the combinational groups containing only hydrophilic compounds did not always show synergistic effects. Therefore, the compatibility of diets indicates balancing the ratios of hydrophilic and lipophlic compounds in our daily food. In addition, the expression of enzymes (SOD, GSH-Px, and CAT) may not be sensitive to the changes of antioxidant activity caused by the combinations with different ratios of hydrophilic and lipophilc compounds. The different structures of lipophilic compounds (β-carotene and lycopene) could influence the antagonistic effects.
研究领域:
营养学
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生物化学
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食品科学
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细胞生物学
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ENVIRONMENTAL POLLUTION
·
2026
·
IF 7.3
Shushu Song,Qinghao Wei,Ying Tu,Shun Zhou,Jiaojiao Zhou,Junyan Tao,Yun Chen,Hao Zhou,Mingdan You
The risk of autism spectrum disorder (ASD) is elevated by exposure to the organophosphate pesticide chlorpyrifos (CPF) during pregnancy and lactation. As a neurodevelopmental disorder, ASD often co-occurs with depression, exacerbating the social isolation experienced by affected individuals. Trehalose (Tre), a naturally occurring non-reducing sugar, has neuroprotective properties. Nevertheless, the mechanisms by which it exerts protective effects against CPF-induced ASD and depression-like behaviors remain unclear. The objective of study was to investigate the mechanisms underlying Tre combating CPF-induced ASD and depression through network pharmacology and toxicology analyses. In animal studies, Tre was administered to offspring mice exposed to CPF during pregnancy and lactation. Behavioral assessments revealed that Tre alleviated CPF-induced repetitive and stereotypic behaviors, social deficits, and depression-like behaviors in offspring mice. Findings from network pharmacology and toxicology analyses and animal experiments supported the notion that mammalian target of rapamycin-related autophagy and TLR4-related necroptosis signaling may contribute to the protective effects of Tre against CPF-induced hippocampal damage. This research offers valuable information regarding the potential protective effects of Tre against neurodevelopmental disorders linked to environmental chemicals.
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TEXTILE RESEARCH JOURNAL
·
2025
·
IF 1.9
Yunlong Zhu,Fang Sun
In the context of the global pandemic, the demand for antimicrobial and antiviral functional textiles has risen sharply, driving the pursuit of more efficient and durable surface modification strategies. In this study, we propose a simple and effective two-step impregnation method to simultaneously enhance the antibacterial and antiviral properties of cotton fabrics. Specifically, methyl thioglycolate (METG) was first covalently grafted onto a cotton fiber surface, followed by stable immobilization of Cu(II) ions through coordination with the introduced thiol groups. The resulting fabric exhibited outstanding antimicrobial and antiviral performance, achieving a 99.99% bacterial reduction rate (BR) against both Escherichia coli and Streptococcus aureus within 20 minutes (AATCC 100-2012 Test Standard), and a 99.99% virus inactivation rate (VR) against bacteriophage Phi-x174 within 5 minutes (YY/T 1497-2016 Test Standard). Notably, the modified fabric maintained excellent antimicrobial and antiviral performance even after 400 abrasion cycles or 50 washing cycles. Moreover, the intrinsic properties of the modified cotton fabric (including water vapor permeability, tensile strength, water absorption, droplet diffusion area, and softness) remained essentially unchanged, ensuring comfort and wearability. This method offers several advantages: it is easy to operate, cost-effective, environmentally friendly, and well-aligned with the principles of sustainable development. Additionally, it provides a promising platform for developing high-performance antimicrobial and antiviral textiles, especially for use in healthcare and personal protection.
研究领域:
纺织科学
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材料科学
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纳米技术
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表面工程
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生物技术
·
化学工程
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高影响因子
Journal of Media Business Studies
·
2021
·
IF 0
Dam Hee Kim
Although the film industry is high risk, sequels and films adapted from novels or comic books can be financially successful because they can capitalise on the established brands of their parent work. Focusing on sequels and adaptations as extended lines and brands, this paper analysed 2,490 films released between 2010 and 2013 in the U.S. to examine what types of films were successful in terms of opening weekend gross and non-opening gross. During opening weekends, while sequels generated higher grosses than non-sequels, sequels adapted from existing work generated even higher grosses. Brand strategies involving stars and title novelty further helped adaptation sequels generate higher grosses mostly during opening weekends. Sequels and adaptations may work together to garner line and brand extension benefits upfront.
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Food Bioscience
·
2024
·
IF 4.8
Bo Yang,Shijiao Liu,Hongwen Zang,Yiwei Dai,Sufang Zhang,Xinping Lin,Huipeng Liang,Yingxi Chen
In order to explore novel producing strategies for enhancing flavor compounds profile and quality of strawberry wine, we studied the effect of the sequential fermentation of two selected indigenous non- Saccharomyces yeasts ( Hanseniaspora uvarum PGR9 and Kurtzmaniella quercitrusa PGT1) and Saccharomyces cerevisiae CGMCC 2.3854 on the fermentation kinetics, physicochemical characteristics, organic acids, antioxidant activity, volatile compositions, and sensory properties. Results showed that the ethanol content produced by the fermentation of Kq-Sc was significantly lower than that of Sc pure fermentation. In addition, the total volatile compounds concentration produced by the fermentation of Hu-Sc (16.2 ± 3.6 mg/L) and Kq - Sc (19.5 ± 3.1 mg/L) were significantly higher than that of Sc pure fermentation (13.8 ± 2.0 mg/L). In particular, the Kq-Sc strawberry wine presented the highest number of relative odor-active compounds (rOAV ≥1). Moreover, strawberry wine fermented by Hu + Kq-Sc showed the highest ABTS free radical scavenging ability and the highest overall sensory qualities. The results indicated that the K. quercitrusa PGT1 strain could be an alternative yeast for producing strawberry wine, and the sequential fermentations of Kq-Sc and Hu + Kq-Sc were effective strategies for improving the flavor and quality of strawberry wine.
研究领域:
食品科学
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微生物学
·
酿酒学
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危险品化学品经营许可证（带存储）