COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2026
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IF 5.4
Ting Zhang,Lu Zhang,Jiaojiao Qiao,Qingsong Li,Ding Wei,Jianpeng Zhang,Yi Wang
Attapulgite-waste newspaper cellulose aerogel (ATP-WNCA) was fabricated. • ATP-WNCA got 99.9% adsorption removal ratio of MB. • ATP-WNCA got an MB adsorption capacity of 59.61 mg/g (6.5 times that of pure WNCA). • Due to its unique 3D porous structure, ATP-WNCA has multiple adsorption mechanisms. In this study, waste newspaper cellulose (WNC) was extracted from waste newspapers and compounded with natural attapulgite (ATP) to develop a novel three-dimensional porous composite material attapulgite-waste newspaper cellulose aerogel (ATP-WNCA). Cellulose was obtained from waste newspapers through an acid-base combined treatment, and ATP-WNCA was successfully prepared using a sol-gel method with a freeze-drying process. The prepared aerogel material was characterized by XRD, FT-IR, and XPS techniques, and the results indicated that in ATP-WNCA, the rod like fiber of attapulgite is used as the skeleton, and cross-linked with cellulose to form aerogel. Its thermal stability was significantly enhanced, and its specific surface area was dramatically increased from 9.56 m²/g of WNC to 127.91 m²/g of ATP-WNCA. The adsorption performance experiments of ATP-WNCA for methylene blue (MB) demonstrated that under the optimal conditions of adsorbent dosage of 900 mg/L, temperature of 30°C, and pH of 11, ATP-WNCA got 99.9% adsorption removal ratio of MB, with an adsorption capacity of 59.61 mg/g (6.5 times that of pure WNCA). According to the experimental and adsorption isotherm fitting and kinetic fitting results, the adsorption mechanism mainly includes electrostatic adsorption, ion exchange, pore filling, hydrogen bonding, and surface complexation. Different from prior waste cellulose adsorbents suffering from low surface area, poor thermal resistance, this work innovatively employs natural ATP as a reinforcing skeleton to overcome the inherent limitations of pure waste newspaper cellulose aerogels; the ATP-WNCA exhibits competitive MB capture performance against some reported composite adsorbents and establishes a facile, cost-effective and sustainable strategy for converting waste paper into high-efficiency organic dye remediation materials, substantially advancing the development of biomass-clay composite adsorbents for practical wastewater treatment. Download: Download high-res image (366KB) Download: Download full-size image
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DOI
高影响因子
CARBON
·
2026
·
IF 12.7
Meng Cai,Can He,Xiaoqiang Fan,Kaiyun Wang,Minhao Zhu
Strong interfacial interactions between nanofillers and resin molecules are crucial for enhancing the corrosion/wear resistance of composite coating. Herein, an interface engineering strategy that integrates Ti 3 C 2 T x with attapulgite nanofibers to create a hierarchical 2D/1D heterostructure (P–Ti 3 C 2 T x @ATP) is reported. The as-prepared P–Ti 3 C 2 T x @ATP with woolen-like structure is incorporated into epoxy resin coating to enhance the anticorrosion and tribological properties. The corresponding enhancement mechanisms are thoroughly investigated. The results indicate that the energy storage modulus of composite coating with P–Ti 3 C 2 T x @ATP (PMX@ATP) is increased by 972 MPa compared to pure epoxy resin coating (EP). The PMX@ATP shows reductions of 0.12 in friction coefficient and 77% in wear rate. And its coating resistance (2.97 × 10 6 Ω cm 2 ) surpasses that of EP (2.70 × 10 5 Ω cm 2 ) by an order of magnitude after 21 days of immersion. The woolen-like P–Ti 3 C 2 T x @ATP has strong interfacial interactions with resin molecules, which exerts the barrier effect of Ti 3 C 2 T x and improves the resistance of composite coating to plastic deformation. This work provides a new idea for the design of MXene-based coatings. Download: Download high-res image (265KB) Download: Download full-size image
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高影响因子
BIOMATERIALS
·
2025
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IF 12.9
Xinghua Yu,Lingan Zeng,Haiyan Chen,Xuemei Dong,Fan Wen,Mingming Wang,Runqi Pan,Yujun Zhang,Wei Zhu,Dingyuan Yan,Dong Wang,Fei Sun
Asthenozoospermia, a leading cause of male infertility characterized by impaired sperm motility, poses significant therapeutic challenges due to the restrictive blood-testis barrier (BTB). To address this limitation, we engineered multifunctional extracellular vesicles (EVs) derived from umbilical cord mesenchymal stem cells, loaded with adenosine triphosphate (ATP) to synergistically restore cellular bioenergetics and suppress ferroptosis. These EVs were further functionalized with an aggregation-induced emission luminogen for near-infrared-II (NIR-II) fluorescence imaging, enabling real-time visualization of their efficient BTB penetration and targeted accumulation within testicular tissues. Upon localization in the seminiferous tubules, the released ATP directly replenishes the energy reserves of spermatogenic cells and enhances sperm motility in asthenozoospermia models. Simultaneously, the EVs upregulate glutathione peroxidase 4, mitigating ferroptosis and synergizing with ATP to restore metabolic homeostasis. In vivo studies demonstrate the dual efficacy of this platform: the EVs precisely traverse the BTB while robustly inhibiting ferroptosis without systemic toxicity, significantly improving sperm count and kinematic parameters (motility, velocity). This study presents a multifunctional nanoplatform that integrates NIR–II–guided imaging, ATP-mediated energy restoration, and pathology-specific ferroptosis inhibition, providing a promising non-invasive therapeutic strategy for asthenozoospermia.
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DOI
PubMed
高影响因子
Bioactive Materials
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2024
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IF 18
Xiaoliang Qi,Yajing Xiang,Ying Li,Jiajia Wang,Yuxi Chen,Yulong Lan,Jinsong Liu,Jianliang Shen
Adenosine triphosphate (ATP)-activated prodrug approaches demonstrate potential in antibacterial uses. However, their efficacy frequently faces obstacles due to uncontrolled premature activation and spatiotemporal distribution differences under physiological circumstances. Herein, we present an endogenous ATP-activated prodrug system (termed ISD3) consisting of nanoparticles (indole-3-acetic acid/zeolitic imidazolate framework-8@polydopamine@platinum, IZPP) embedded in a silk fibroin-based hydrogel, aimed at treating multidrug-resistant (MDR) bacteria-infected pressure ulcers. Initially, an ultraviolet-triggered adhesive ISD3 barrier is formed over the pressure ulcer wound by a simple local injection. Subsequently, the bacteria-secreted ATP prompts the degradation of IZPP, allowing the loaded IAA prodrug and nanozyme to encounter spatiotemporally on a single carrier, thereby efficiently generating reactive oxygen species (ROS). Exposure to 808 nm near-infrared light enhances the catalytic reaction speed, boosting ROS levels for stronger antibacterial action. Once optimal antibacterial action is reached, ISD3 switches to a dormant state, halting any further ROS production. Moreover, the bioactive components in ISD3 can exert anti-inflammatory functions, aiding in pressure ulcer recovery. Overall, our research introduces a hydrogel prodrug strategy activated by bacterial endogenous ATP, which precisely manages ROS generation and accelerates the recovery of MDR bacteria-infected pressure ulcers.
研究领域:
生物医学工程
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微生物学
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药剂学
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高影响因子
JOURNAL OF CONTROLLED RELEASE
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2025
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IF 11.5
Min Gao,Yue Song,Jing Liang,Tiantian Chen,Jiajia Luo,Panyu Du,Han Wang,Hongyu Leng,Zheng Wang,Xinlong Ma,Kai Wang,Yanjun Zhao
Concurrent induction of apoptosis and ferroptosis is promising in handling heterogenous cancers. We report a tailored polymeric micellar nanoplatform for the combinational anti-tumor therapy. Two stimuli-responsive amphiphlic block copolymers were synthesized, bearing three functional moieties, i.e. azobenzene, nitroimidazole and 3-fluorophenylboronic acid. Azobenzene could enhance the cellular uptake of micelles. Nitroimidazole and 3-fluorophenylboronic acid could deplete the reduced nicotinamide adenine dinucleotide phosphate (NADPH), glucose and adenosine triphosphate (ATP) under hypoxia, sensitizing ferroptotic and apoptotic cell death. The proof-of-concept was demonstrated in a triple-negative breast cancer cell line (MDA-MB-231). Irrespective of the free or encapsulated form, doxorubicin and auranofin showed a synergistic action, evidenced by a low combination index (< 1). The co-delivery micelles showed improved potency than the single drug-loaded micelles in terms of the biomarkers of apoptosis ( e.g. caspase 3/9, cytochrome c and ATP) and ferroptosis ( e.g. thioredoxin reductase, thioredoxin, glutathione, NADPH, malondialdehyde and lipid peroxides). The apoptosis and ferroptosis induction ability of cargo-free micelles was proved. The in vivo efficacy was verified in the MDA-MB 231 tumor-bearing nude mice model. The current work offers a promising strategy of combinational anti-tumor drug delivery for potent induction of ferroptosis and apoptosis via the sensitization effect of vehicle in the hypoxic tumor.
研究领域:
生物医学工程
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肿瘤学
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药剂学
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产品关联
DOI
PubMed
高影响因子
BIOMACROMOLECULES
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2018
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IF 5.738
Zhanwei Zhou,Minghua Zhang,Yadong Liu,Chenzi Li,Qingyan Zhang,David Oupicky,Minjie Sun
Cationic polyplex as commonly used nucleic acid carriers faced several shortcomings, such as high cytotoxicity, low serum stability, and slow cargo release at the target site. The traditional solution is covering a negative charged layer (e.g., hyaluronic acid, HA) via electrostatic interaction. However, it was far from satisfactory for the deshielding by physiological anions in circulation (e.g., serum proteins, phosphate). In this study, we proposed a new strategy of reversible covalent cross-linking to enhance stability in circulation and enable stimuli-disassembly of polyplexes in tumor cells. Here, 25k polyethylenimine (PEI) was chosen as model polycations for veriying the hypothesis. HA-PEI conjugation was formed by the cross-linking of adenosine triphosphate grafted HA (HA-ATP) with phenylboronic acid grafted PEI (PEI–PBA) via the chemical reaction between PBA and ATP. Compared with noncovalent polyplex by electrostatic interaction (HA/PEI), HA-PEI exhibited much better colloidal stability and serum stability. The covered HA-ATP layer on PEI–PBA could maintain stable in the absence of physiological anions, while HA layer on PEI in HA/PEI group showed obvious detachment after anion’s competition. More importantly, the covalent cross-linking polyplex could selectively release siRNA in the ATP rich environment of cytosol and significantly improve siRNA silence. Besides, the covalent cross-linking with HA-ATP could effectively reduce the cytotoxicity of cationic polyplex, improve the uptake by B61F10 cells and promote the endosomal escape. Consequently, this strategy of HA-PEI conjugation significantly enhanced the siRNA transfection in the absence or presence of FBS (fetal bovine serum) on B16F10 cells and CHO cells. Taken together, the reversible covalent cross-linking approach shows obvious superiority compared with the noncovalent absorption strategy. It held great potential to be developed to polish up the performance of cationic polyplex on reducing the toxicity, enhancing the serum tolerance and achieving controlled release of siRNA at target site.
研究领域:
药学
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生物技术
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纳米医学
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分子生物学
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DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
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2022
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IF 8.008
Min Jiang,Xiaoxue Xi,Zhen Wu,Xiuhua Zhang,Shengfu Wang,Wei Wen
In situ and quantitative measurements of adenosine 5′-triphosphate (ATP) in single living cells are highly desired for understanding several sorts of necessary physiological and pathological processes. Due to its small size and high sensitivity, an ultra-microelectrode can be used for single-cell analysis. However, ATP is difficult to detect in single cells because it is nonelectroactive and low in content. Herein, we introduced an electrochemical nano-biosensor based on an amphiphilic aptamer-assisted carbon fiber nanoelectrode (aptCFNE) with high signal-to-noise ratio. The low current (e.g., 60 pA) and the tiny diameter of the tip (ca. 400 nm) of the nanosensor made it noninvasive to living cells. The amphiphilic aptamer has good biocompatibility and can be stably modified to the surface of functionalized electrodes. CFNE, which was modified with ferrocene-labeled aptamer, could quickly and selectively detect ATP content in the nucleus, cytoplasm, and extracellular space of single HeLa cells. The results showed that the ATP contents in the nucleus, cytoplasm, and extracellular space were 568 ± 9, 461 ± 20, and 312 ± 4 μM, respectively. The anticancer drug treatment effects on the cellular level were further recorded, which was of great significance for understanding ATP-related biological processes and drug screenings. This strategy is universally applicable to detect other targets by changing the aptamer sequence, which will greatly improve our understanding of cell heterogeneity and provide a more reliable scientific basis for exploring major diseases at the single-cell level.
研究领域:
分析化学
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生物化学
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生物医学工程
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药理学
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DOI
PubMed
高影响因子
DYES AND PIGMENTS
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2026
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IF 3.9
Wenlong Xing,Yin Zhu,Jiahao Wu,Shijia Li,Zhenyu Zhao,Jinsong Zhang,Shaowei Bo,Ji Zeng,Jiangling Peng
The rapid and accurate detection of adenosine 5′-triphosphate (ATP) is crucial for clinical diagnostics, bioanalysis, and food safety. This study presents two novel aggregation-induced emission (AIE)-active probes, BIP-PP-1 and BIP-PP-2 , derived from a new N-heterocyclic AIEgen core, benzo[4,5]imidazo[1,2- c ]pyrimidine (BIP). The optimized probe, BIP-PP-2 , functions as a high-performance ATP sensor with a large Stokes shift, high sensitivity (LOD = 0.85 μM, linear range 0 – 90 μM), and excellent selectivity over structurally similar anions such as ADP, AMP, CTP, TTP, and UTP, a feature seldom achieved in small-molecule ATP probes. This unique selectivity is attributed to the novel BIP core, where specific π-π stacking and hydrogen-bonding interactions with the adenine moiety of ATP provide a superior stereoelectronic match, driving precise recognition and AIE-based fluorescence enhancement via a synergistic combination of electrostatic and non-covalent forces. BIP-PP-2 was successfully applied in real-time ATP imaging within live bacterial cells and, notably, in the rapid visual detection of bacterial contamination in beverages using a portable 365 nm UV lamp, achieving a visual detection limit of 3.9 × 10 5 CFU mL −1 under ambient light. This work introduces the BIP scaffold as a promising platform for functional dye design and establishes BIP-PP-2 as a robust analytical tool for on-site bioanalysis and food safety monitoring. © 2001 Elsevier Science. All rights reserved.
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高影响因子
MICROCHEMICAL JOURNAL
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2019
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IF 3.206
Wen Nie,Fangfang Wang,Runqing Hao,Lu Zhang,Qian Chen,Fanmei Wei,Xiaotong Li,Robert Loffredo,Zhenbo Liu,Yongming Liu
For aqueous two-phase extraction (ATPE), adsorbents such as primary secondary amine (PSA) are usually used to remove fat and protein in meat samples. However, it was determined that PSA adsorbed approximately 5% of measured sulfonamides (SAs), which is the reason for the low recovery rate. To overcome this adverse PSA effect, a solid phase extraction (SPE) method was used for purification and enrichment after the formation of aqueous two-phase. Based on these, A new aqueous two-phase sample pretreatment method with solid phase extraction (ATP-SPE) to detect sulfonamides in fish meat is proposed. Fluorescence quenching showed that for 6 SAs, hydrogen bonding and van der Waals force played the major role in the binding reaction with fish serum albumin protein. In addition, in the 80% ACN aqueous solution, a slow protein denaturation took place and the drugs bound with the protein were released, which is more conducive to the aqueous two-phase extraction. In order to obtain better separation effect, the factors for synthesis of hydrophobic-lyophobic balance (HLB) packing for SPE were explored. Stirring speed is the most important factor affecting the particle size of microspheres . After purification and enrichment by SPE, samples were directly analyzed by HPLC. The analytical process was validated in the fish sample matrix by the analysis of spiked blank samples. The mean recoveries of this method were 80.1–95.1% with RSD values of 1.37–3.95%. The detection limits were 5.8–11.7 ng/g. The new ATP-SPE pretreatment method provides a new way for better recovery of veterinary drugs in meat products.
研究领域:
分析化学
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食品科学
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药理学
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DOI
高影响因子
ACS Applied Materials & Interfaces
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2023
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IF 9.5
Zhezhu Nan,Hengyu Liu,Linrong Shi,Hongrui Zhu,Junjie Chen,Tali Ilovitsh,Daocheng Wu,Mingxi Wan,Yi Feng
Focused ultrasound, as a protocol of cancer therapy, might induce extracellular adenosine triphosphate (ATP) release, which could enhance cancer immunotherapy and be monitored as a therapeutic marker. To achieve an ATP-detecting probe resistant to ultrasound irradiation, we constructed a Cu/N-doped carbon nanosphere (CNS), which has two fluorescence (FL) emissions at 438 and 578 nm to detect ultrasound-regulated ATP release. The addition of ATP to Cu/N-doped CNS was conducted to recover the FL intensity at 438 nm, where ATP enhanced the FL intensity probably via intramolecular charge transfer (ICT) primarily and hydrogen-bond-induced emission (HBIE) secondarily. The ratiometric probe was sensitive to detect micro ATP (0.2–0.6 μM) with the limit of detection (LOD) of 0.068 μM. The detection of ultrasound-regulated ATP release by Cu,N-CNS/RhB showed that ATP release was enhanced by the long-pulsed ultrasound irradiation at 1.1 MHz (+37%, p < 0.01) and reduced by the short-pulsed ultrasound irradiation at 5 MHz (−78%, p < 0.001). Moreover, no significant difference in ATP release was detected between the control group and the dual-frequency ultrasound irradiation group (+4%). It is consistent with the results of ATP detection by the ATP-kit. Besides, all-ATP detection was developed to prove that the CNS had ultrasound-resistant properties, which means it could bear the irradiation of focused ultrasound in different patterns and detect all-ATP in real time. In the study, the ultrasound-resistant probe has the advantages of simple preparation, high specificity, low limit of detection, good biocompatibility, and cell imaging ability. It has great potential to act as a multifunctional ultrasound theranostic agent for simultaneous ultrasound therapy, ATP detection, and monitoring.
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2023
·
IF 4.4
Jabrane Jouha,Fengli Li,Hai Xiong
Due to their distinctive physical, chemical, electrical, and optical properties as well as their prospective uses, 2D covalent organic framework (COF) have attracted much attention. Herein, TaTPA-COF was effectively synthesized from the condensation of TTA and TFPA using a facile solvothermal method and characterized by SEM image, FT-IR spectra, and PXRD pattern. The generated bulk TaTPA-COF materials combined with DNA aptamers are utilized as the acceptor (quencher) for the highly sensitive and selective detection of adenosine 5′-triphosphate (ATP) and thrombin, with a novel fluorescence biosensing platform and a proof-of-concept application.
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Lei Luo,Min Wang,Yuan Zhou,Dongliu Xiang,Qing Wang,Jin Huang,Jianbo Liu,Xiaohai Yang,Kemin Wang
For intracellular molecular detection, the appropriate probes should include the abilities to enter target cells noninvasively, target specific sites, and then respond to the analytes reliably. Herein, a ratiometric fluorescent DNA nanostructure (RFDN) was designed for mitochondrial adenosine triphosphate (ATP) imaging in living cells. The DNA nanostructure was constructed by continuous hybridization of two hairpin DNA strands (HS1-Cy3 and HS2-Cy5) under the initiation of the trigger. HS1-Cy3 and HS2-Cy5 contained split aptamer fragments of ATP and are labeled with a fluorescent donor (Cy3) and acceptor (Cy5), respectively. The RFDN integrated multiple split aptamer fragments and increased the local concentration of sensing probes. The binding of ATP to aptamer fragments on the RFDN shortened the distance between Cy3 and Cy5, resulting in obvious ratiometric signals (fluorescence resonance energy transfer). The RFDN showed good biocompatibility and can be internalized into cells in a caveolin-dependent endocytosis pathway. The co-localization imaging results indicated that the DNA nanostructure could target the mitochondria via Cy3 and Cy5. Moreover, the confocal imaging results showed that the intracellular ATP changes stimulated by drugs in living cells could be indicated by the RFDN. In this way, the RFDN is expected to be a simple, flexible, and general platform for chemo/biosensing in living cells.
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）