MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Yansheng Liu,Yunjie Zhan,Haonan Hu,Guofu Wang,Xiaobo Jia,Jin Zhou,Hongqi Li,Haixin Chang,Zhaoxu Li
This study reports a SERS-based microfluidic chip for the cyclic detection of histamine with high specificity. Easily fabricated Au nanoparticles (AuNPs) were synthesized on Si substrates by using a thermally treating approach. The designed probe DNA (pDNA) functionalized AuNPs is used as a sensing surface. The Cyanine 3 (Cy3) functionalized complementary DNA (cDNA) is modified on the chemically synthesized AuNPs and the complex cDNA/AuNPs are used as nanotags. Due to the hybridization between the pDNA and the cDNA, nanotags-pDNA/AuNPs structure is formed and illustrates a high SERS intensity of Cy3. With the presence of the histamine, due to the stronger affinity between cDNA and histamine, nanotags-pDNA/AuNPs structure is destroyed and the nanotags are separated from the sensing surface resulting in a decrease in the SERS intensity of Cy3. By adding fresh nanotags, nanotags-pDNA/AuNPs structure is regenerated, and a new detection cycle can be performed. By utilizing the proposed SERS-based microfluidic chip, the limit of detection (LOD) of histamine in DI water is 1 × 10 −9 g/mL. The concentration is well below the U.S. Food and Drug Administration (FDA) histamine toxicity threshold of 5 × 10 −5 g/mL. Moreover, the proposed SERS-based microfluidic chip can be reused 7 times. Furthermore, this chip has accuracy and applicability were validated through its application in beer samples. Indicating that this method has great potential for histamine detection.
研究领域:
分析化学
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生物传感器
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纳米技术
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微流控
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食品安全
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DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2025
·
IF 19
Wen Zhang,Wenchao Chen,Xiaoqin Ma,Yanfu Feng,Xiangpeng Geng,Qin Gong,Dekai Guan,Yaxin Liu,Bin Ma
The thermal management of highly integrated chips under high thermal flux density is hindered by disordered high-thermal-conductivity matrices that reduce phonon transport efficiency. To address this challenge, this study employs directional freezing to systematically align multi-walled carbon nanotubes, thereby establishing efficient thermal conduction pathways. Subsequent vacuum impregnation with the CaCl 2 ·6H 2 O phase change material (PCM) yields a composite material with pronounced anisotropic thermal conductivity—6.86 W·m −1 ·K −1 along the axial direction and 0.08 W·m −1 ·K −1 along the radial direction. This structural design not only enhances axial heat transfer and accelerates thermal equilibrium, but it also significantly suppresses PCM leakage via nanoconfinement and capillary effects, accelerating its phase transition by reducing subcooling to 11.41 °C. When employed for the transient thermal management of CPUs, the proposed material effectively directs heat flow, resulting in a 24 °C reduction in peak core temperature and a 19.5 °C reduction in peak surface temperature. Furthermore, this material demonstrates excellent thermal stability over 20 thermal cycles, with a maximum temperature fluctuation of only 5.2 °C. This study thus presents an effective strategy for the development of high-performance, structurally controllable PCM-based thermal interface materials.
研究领域:
材料科学
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热工工程
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电子器件散热
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纳米复合材料
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能量存储与管理
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DOI
高影响因子
TALANTA
·
2025
·
IF 6.1
Songbai Tian,Tingting Xiang,Xinghu Ji,Fuxiang Zhou,Zhike He
Digital immunoassay is a valuable method for quantifying protein at the single-molecule level. Traditional digital immunoassay (the single-molecule arrays, SiMoAs) involves distributing the antibody-antigen-antibody sandwich immune complex into droplets, but some targets are lost during this process. To address this issue, we developed a magnetic microparticle anchoring-and-release digital immunoassay method for protein detection. We embedded a commercial track-etched polycarbonate (PCTE) membrane into the microfluidic chip for droplet generation, and modified the magnetic microparticles (MMPs) with enzymes for signal production. This method involves two processes: first, the enzymes modified MMPs are anchored in 96-well plates through the target protein. Second, the enzymes modified MMPs were released, and digitally quantified in the membrane-based microfluidic chip. This magnetic microparticle anchoring-and-released digital immunoassay method was successfully applied to the detection of p24 protein. Results showed that p24 could be digitally detected within the range of 1–100 pg/mL with good selectivity and accuracy using this digital method. By reducing signal loss during the digital immunoassay process, this method represents a significant advancement in digital technology.
研究领域:
生物分析化学
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微流控技术
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免疫学
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生物传感器
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分子诊断学
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2025
·
IF 5.7
Ming-Yue Gao,Meng Wang,Yong-Tao Wang,Zhi-Ling Zhang
Nanozymes, the nanomaterials with enzyme-like characteristics which exhibit lower cost, easier synthesis and functionalization, better stability compared with natural enzymes, have been widely developed for biosensing, disease therapy and environmental governance. However, the lack of catalytic efficiency of nanozymes compared to natural enzymes makes it difficult to completely replace natural enzymes to achieve higher sensitivity and lower detection limit in biosensing. Herein, magnetism-controlled technology was used to form nanozyme array consisted of stacked Fe3O4/Au NPs at the bottom of the microchannel as a spatially confined microreactor for the catalytic reaction. By enhancing the mass transfer process of substrate towards nanozyme which was mediated by the corresponding V-structure, a higher local concentration of substrate and more efficient utilization of active sites of nanozyme were obtained to increase the catalytic efficiency (kcat/KM) of the nanozyme array consisted of the Fe3O4/Au NPs by 95.2%, which was two orders of magnitude than that of the open reactor. Based on this, a colorimetric method on an integrated microfluidic platform was proposed for sensitive biosensing of Salmonella typhimurium. The entire detection could be completed within 30 minutes, yielding a linear range from 102 to 107 CFU/mL and a detection limit as low as 5.6 CFU/mL.
研究领域:
生物传感
·
纳米材料科学
·
微生物检测
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产品关联
DOI
PubMed
高影响因子
ANALYST
·
2025
·
IF 3.3
Die Hu,Jiawen Xiang,Jingying Guo,Chao Wang,Ji Qi,Bowei Li,Xiaoyan Wang,Xin Zhang,Lingxin Chen,Xuming Zhuang
In this study, we developed a simple and cost-effective paper and cloth-based microfluidic fluorescence sensing device for the selective and quantitative detection of L-cysteine (L-Cys) in deep-sea water, addressing the need for efficient monitoring of this critical metabolite of deep-sea creature’s biomarker. The device employs gold nanoparticles (Au NPs) immobilized on a cloth-based substrate and Rhodamine B (Rh B) molecules. In the presence of L-Cys, strong interactions between L-Cys and the Au NPs release Rh B molecules, restoring fluorescence proportional to the cysteine concentration. The device achieves a low detection limit of 0.52 nM with a dynamic range of 1-100 nM. It demonstrates excellent selectivity over other amino acids, stability over 30 days, and reproducibility. Its practical applicability was confirmed using deep-sea cold seep water samples, yielding recoveries of 98.07%-102.62%. Compared to existing methods, this platform offers enhanced portability, lower cost, and faster response, making it suitable for in-situ environmental monitoring.
研究领域:
分析化学
·
环境科学
·
海洋生物学
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产品关联
DOI
PubMed
高影响因子
LAB ON A CHIP
·
2024
·
IF 6.1
Yushen Wang,Junlei Han,Wenteng Tang,Xiaolong Zhang,Jiemeng Ding,Zhipeng Xu,Wei Song,Xinyu Li,Li Wang
Microplastics (MPs) are pervasive pollutants present in various environments. They have the capability to infiltrate the human gastrointestinal tract through avenues like water and food, and ultimately accumulating within the liver. However, due to the absence of reliable platforms, the transportation, uptake, and damage of microplastics in the gut-liver axis remain unclear. Here, we present the development of a gut-liver-on-a-chip (GLOC) featuring biomimetic intestinal peristalsis and a dynamic hepatic flow environment, exploring the translocation in the intestines and accumulation in the liver of MPs following oral ingestion. In comparison to conventional co-culture platforms, this chip has the capability to mimic essential physical microenvironments found within the intestines and liver (e.g., intestinal peristalsis and liver blood flow). It effectively reproduces the physiological characteristics of the intestine and liver (e.g., intestinal barrier and liver metabolism). Moreover, we infused polyethylene MPs with a diameter of 100 nm into the intestinal and hepatic chambers (concentrations ranging from 0 to 1 mg mL−1). We observed that as intestinal peristalsis increased (0%, 1%, 3%, 5%), the transport rate of MPs decreased, while the levels of oxidative stress and damage in hepatic cells decreased correspondingly. Our GLOC elucidates the process of MP transport in the intestine and uptake in the liver following oral ingestion. It underscores the critical role of intestinal peristalsis in protecting the liver from damage, and provides a novel research platform for assessing the organ-specific effects of MPs.
CAS / MDL:
研究领域:
环境科学
·
生物医学工程
·
毒理学
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产品关联
DOI
PubMed
高影响因子
ACS Omega
·
2024
·
IF 3.7
Lanfeng Guo,Renlong Liu,Zhaobo He,Shaoping Li,Tong Tan,Changyuan Tao
The copper connectivity technique is essential for achieving electrical interconnection in wafer level packaging (WLP), system in packaging (SiP), and 3D packaging. The essential processing material for copper connectivity is a copper sulfate electroplating solution in which organic additives play a crucial role in the regularity of copper electrodeposition. In this study, electrochemical tests, X-ray diffraction, 3D profiling, and scanning electron microscopy were used to investigate the leveling effect and mechanism of polyquaternary ammonium urea-containing polymer (PUB2) in the process of copper electrodeposition on-chip copper connections. PUB2 has excellent polarization ability on the target surface, remains unaffected by the sulfur additive SPS and poly(ethylene glycol), and displays a strong ability to regulate the copper deposition rate of through-holes and surface wiring. The waviness of the wafer surface wiring was reduced from 130 to approximately 70 nm after optimizing the PUB2 concentration, and the surface roughness was reduced from 10 to approximately 7 nm. The coating was dispersed evenly, and the rate of through-hole filling was improved by 57%. This study not only examined PUB2 leveling performance and mechanisms but also devised a research method and system for electroplating additives to facilitate the development and application of new electroplating additives.
研究领域:
材料科学
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电子工程
·
电化学
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2024
·Vol. 16
·期 39
·6736-6745
·
IF 2.7
Shuai Yuan
Water environments contaminated by heavy metal ions present significant challenges because these pollutants do not degrade naturally, leading to their gradual bioaccumulation in animals and plants, which ultimately poses an insurmountable threat to human health. Therefore, rapid and accurate detection of heavy metal ions in water is of great significance for environmental protection and disease prevention. In this work, we developed a novel method based on microfluidic electrophoresis coupled with indirect chemiluminescence for the immediate detection of Cd(II), Pb(II) and Hg(II) heavy metal ions. The displacement of the Co(II) ions within the chemiluminescence mixture by the above migrating sample cations caused a measurable reduction in the background signal. The results showed that the detection limits of Cd(II), Pb(II) and Hg(II) ions under the best detection conditions were 5.83 × 10−8 M, 5.38 × 10−8 M and 2.09 × 10−8 M, respectively, which were 1–2 orders of magnitude lower than those of the indirect UV method and 1 order of magnitude lower than that of the indirect laser induced detection method. This method can provide a possibility for the rapid detection of multiple heavy metal ions in actual water environments.
研究领域:
环境科学
·
分析化学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Yang Yuan,Hui Jia,Jibao Liu,Fei Gao,Xu Zhu,Zihan An,Jie Wang
The development of new low-cost, highly selective and efficient methods for the separation and detection of heavy metal ions in aqueous environments is of great importance for water safety and biological health. In this study, a novel ion separation membrane was prepared for the separation of copper ions in cadmium ion detection solution. Compared with the commercial membrane, the ion mobility was increased by 64.02 %, and further separated by selective complexation of thiacalix[4]arene-p-tetrasulfonate (TCAS), and the separation factor for Cu/Cd reached 34.66. This allowed Cd to be detected preferentially, and reduced the interference of other ions in the detection. The joint electric field environment was regulated to separate the organics and reduce the interference to the detection. The sensing performance was enhanced by modifying the screen-printed electrodes. The proposed sensing platform allows for simultaneous pre-processing and detection and has a detection limit of 0.19 μg-L −1 (S/N = 3) for Cd 2+ .
研究领域:
环境科学
·
分析化学
·
生物医学工程
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产品关联
DOI
高影响因子
Biotechnology Journal
·
2024
·Vol. 19
·期 2
·2300390
·
IF 3.2
Yaqiong Guo,Yingying Xie,Jianhua Qin
Organ-on-a-chip technology has shown great potential in disease modeling and drug evaluation. However, traditional organ-on-a-chip devices are mostly pump-dependent with low throughput, which makes it difficult to leverage their advantages. In this study, we have developed a generic, pump-free organ-on-a-chip platform consisting of a 32-unit chip and an adjustable rocker, facilitating high-throughput dynamic cell culture with straightforward operation. By utilizing the rocker to induce periodic fluid forces, we can achieve fluidic conditions similar to those obtained with traditional pump-based systems. Through constructing a gut-on-a-chip model, we observed remarkable enhancements in the expression of barrier-associated proteins and the spatial distribution of differentiated intestinal cells compared to static culture. Furthermore, RNA sequencing analysis unveiled enriched pathways associated with cell proliferation, lipid transport, and drug metabolism, indicating the ability of the platform to mimic critical physiological processes. Additionally, we tested seven drugs that represent a range of high, medium, and low in vivo permeability using this model and found a strong correlation between their P app values and human Fa, demonstrating the capability of this model for drug absorption evaluation. Our findings highlight the potential of this pump-free organ-on-a-chip platform as a valuable tool for advancing drug development and enabling personalized medicine.
研究领域:
生物医学工程
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药理学
·
组织工程
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细胞生物学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 114
·113115-113122
·
IF 3.289
Chunyan Wang,Yi Xu,Conghui Deng,Zhixu Liu,Rong Wang,Huazhou Zhao
In the present paper, we report on the design and fabrication of a recyclable microfluidic SERS chip with an integrated Au@Ag/TiO2 NTs (gold core silver shell nanoparticles (NPs) with decorated TiO2 nanotube arrays (NTs)) composite SERS substrate. The stripped TiO2 NTs with regularly arranged pore structures were prepared on a Ti foil by easy tape lithography followed by anodic oxidation. The Au@Ag NPs were fabricated on the mouth surface and the inner walls of the TiO2 NTs within a microchannel without clogging via a facile self-assembly chemical plating composite method. Under optimized conditions, the microfluidic SERS chip provided excellent sensitivity to R6G molecules with a detection limit of 10−10 M and a SERS enhancement factor (EF) of 1.15 × 108. Versus single Au NPs, Au@Ag NPs and the Au@Ag/TiO2 plate, the optimized Au@Ag/TiO2 NTs showed improved SERS peak intensities for the C–C–C bending (613 cm−1) of 20, 2.3 and 1.6 times enhancement, respectively. The enhancement effect was attributed to both electromagnetic and chemical enhancement. The photocatalytic performance of the Au@Ag/TiO2 NTs was exploited to recycle the microfluidic SERS chip through UV light photocatalytic purification. The experimental result shows that the microfluidic SERS chip featured high reproducibility and could be used as an efficient SERS detector for many biochemical molecules.
研究领域:
材料科学
·
分析化学
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产品关联
DOI
高影响因子
TALANTA
·
2023
·
IF 6.1
Fang Li,Min Zhu,Zimu Li,Nuotong Shen,Hao Peng,Bing Li,Jianbo He
The fabrication of multicolor chemiluminescence (CL) sensing chip for the discrimination and detection of multianalytes remains a great challenge. Herein, machine learning assisted multicolor microfluidic CL detection chip for the identification and concentration prediction of antibiotics was presented. Firstly, a three-channel microfluidic CL detection chip was fabricated. The three detection zones of the microfluidic detection chip were modified with CL catalyst Co(II) and different CL reagents including luminol, luminol mixed with fluorescein , and luminol mixed with phloxine B , respectively. Strong blue, green and pink-purple colored light emissions can be generated from the three detection zones in the presence of H 2 O 2 solution. The three multicolor CL emissions show different degrees of reduce in intensity and change in color in the presence of different antibiotics, including diethylstilbestro (DES), metronidazole (MNZ), kanamycin (KAN), isoniazide (INH), and ceftiofur sodium (CS), resulting in distinct fingerprint-like response patterns. The red (R), green (G), blue (B) and gray scale values of the three multicolor light emissions were extracted and ten characteristic sensing parameters were chosen to obtain multicolor CL response database. Then, machine learning assisted data analysis were carried out. The five antibiotics can be facilely classified by using principal component analysis (PCA) and hierarchical clustering analysis (HCA), and further quantified by using deep neural networks (DNN) algorithm. Good results were obtained for identification of binary antibiotic mixtures, spiked antibiotics in water samples, and unknown antibiotic samples. Satisfied results were obtained for concentration prediction of antibiotics. This work provides a simple machine learning assisted and multicolor microfluidic CL detection chip based CL sensing strategy for discrimination and quantitative detection of multiple analytes.
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DOI
PubMed
高影响因子
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