Journal of Environmental Chemical Engineering
·
2023
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IF 7.7
Yasir Hamid,Lei Liu,Muhammad Haris,Muhammad Usman,Qiang Lin,Yonglong Chen,Muhammad Saqib Rashid,Zaid Ulhassan,M. Iftikhar Hussain,Xiaoe Yang
This study followed the one-step and two-step technologies to produce rice straw biochar (BC), chitosan-modified rice straw biochar (CT-BC), and thiol grafted chitosan-modified biochar (TH@CT-BC) for cadmium (Cd) removal from contaminated water . Here, chitosan and thiol were introduced by two-step combination to enhance the adsorption ability of biochar. Our results revealed the maximum Cd adsorption at pH 5.5 with TH@CT-BC (261.47 mg g −1 ) followed by CT-BC (103.14 mg g −1 ) and BC (29.64 mg g −1 ). Obtained data was best fitted by the Langmuir and pseudo-second-order kinetic models (with R 2 values of 0.997 for TH@CT-BC) as compared to Freundlich and Temkin models (0.949 and 0.925, respectively). TH@CT-BC retained its efficiency in spiked river water system, removing up to 89% of Cd in river water spiked with 30 mg −1 L Cd. The experimental investigations and data calculations demonstrated the surface complexation and electrostatic interaction as dominant underlying mechanisms for Cd removal by TH@CT-BC. Moreover, it is essential to utilize the large amount of produced rice straw to develop new materials which would help in converting the waste to useable product for environmental remediation. Thus, this study demonstrates the production of TH@CT-BC from rice straw as an effective adsorbent of Cd from aqueous systems which can be studied as a potential candidate for practical wastewater treatment applications.
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ARABIAN JOURNAL FOR SCIENCE AND ENGINEERING
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2024
·Vol. 50
·期 13
·10327-10344
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IF 2.6
Xu Ruifeng,Wang Shuoshi,Guo Ping,Hukuang Haoxiang,Yuan Na
The CO 2 -WAG injection method proposed for unconventional tight oil reservoirs has been successfully applied in field. However, for water-sensitive tight oil reservoirs, there is a lack of research focusing on the displacement mechanisms and effects of WAG injection at the pore scale. And the gap between nuclear magnetic resonance (NMR) and computerized tomography (CT) scanning technologies is not well explored. This study integrated deuterium oxide-shielded NMR and contrast agent (n-butane iodide and lead iso-octanoate mixture) enhanced micro-CT to study the immiscible CO 2 -WAG mechanisms for water-sensitive tight oil. The aim is to investigate the microscopic distribution and displacement mechanisms of residual oil after CO 2 flooding and WAG injection in water-sensitive cores. Experimental results showed that NMR experiments and CT experiments identified the microscopic distribution of oil at different injection stages successfully. The contrast agent, despite its similar viscosity ratio, had poor representativeness in other properties, leading to significant differences in the microscopic fluid distribution results compared to NMR experiments. The primary WAG flood mechanism observed was that CO 2 dispersed oil droplets, with most being displaced out of the pores, while water played a mobility control role by blocking large pores. However, due to water sensitivity, medium and small pores also experienced blockage, making the effect of WAG injection after CO 2 flooding not significant. In the NMR experiment, the final recovery of core 6–1 after WAG injection was 64.71%. In the CT scan experiment, due to the smaller volume of the microcore, the water sensitivity effect was relatively weaker, and under the stronger influence of the capillary end effect, the final recovery reached 76.91%. In the five sets of long-core experiments, by controlling the gas–water ratio and slug size, the recovery ranged from 56.51 to 64.04%. The core flood experiments validated the representativeness of the microscopic experiments. And for water-sensitive tight oil reservoirs, controlling the volumes of water and CO 2 during the WAG injection process is crucial.
研究领域:
石油工程
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油气田开发
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渗流力学
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岩石物理
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提高采收率
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高影响因子
BIOCONJUGATE CHEMISTRY
·
2017
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IF 4.818
Ludan Yue,Jinlong Wang,Zhichao Dai,Zunfu Hu,Xue Chen,Yafei Qi,Xiuwen Zheng,Dexin Yu
Multifunctional nanotheranostic agents have been highly commended due to the application to image-guided cancer therapy. Herein, based on the chemically disordered face centered cubic (fcc) FePt nanoparticles (NPs) and graphene oxide (GO), we develop a pH-responsive FePt-based multifunctional theranostic agent for potential in vivo and in vitro dual modal MRI/CT imaging and in situ cancer inhibition. The fcc-FePt will release highly active Fe ions due to the low pH in tumor cells, which would catalyze H2O2 decomposition into reactive oxygen species (ROS) within the cells and further induce cancer cell apoptosis. Conjugated with folic acid (FA), the iron platinum-dimercaptosuccinnic acid/PEGylated graphene oxide-folic acid (FePt-DMSA/GO-PEG-FA) composite nanoassemblies (FePt/GO CNs) could effectively target and show significant toxicity to FA receptor-positive tumor cells, but no obvious toxicity to FA receptor-negative normal cells, which was evaluated by WST-1 assay. The FePt-based multifunctional nanoparticles allow real-time monitoring of Fe release by T2-weighted MRI, and the selective contrast enhancement in CT could be estimated in vivo after injection. The results showed that FePt-based NPs displayed excellent biocompatibility and favorable MRI/CT imaging ability in vivo and in vitro. Meanwhile, the decomposition of FePt will dramatically decrease the T2-weighted MRI signal and increase the ROS signal, which enables real-time and in situ visualized monitoring of Fe release in tumor cells. In addition, the self-sacrificial decomposition of fcc-FePt will be propitious to the self-clearance of the as-prepared FePt-based nanocomposite in vivo. Therefore, the FePt/GO CNs could serve as a potential multifunctional theranostic nanoplatform of MRI/CT imaging guided cancer diagnosis and therapy in the clinic.
研究领域:
生物医学工程
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肿瘤学
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医学影像学
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PubMed
高影响因子
Biomaterials Science
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2024
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IF 5.8
Jinyuan Wang,Xia Sun,Jixuan Xu,Lin Liu,Peng Lin,Xiaofang Luo,Yan Gao,Junpeng Shi,Yun Zhang
As promising luminescence nanoparticles, near-infrared (NIR) persistent luminescence nanoparticles (PLNPs) have received extensive attention in the field of high-sensitivity bioimaging in recent years. However, NIR PLNPs face problems such as short excitation wavelengths and single imaging modes, which limit their applications in vivo reactivated imaging and multimodal imaging. Here, we report for the first time novel Gd2GaTaO7:Cr3+, Yb3+ (GGTO) NIR PLNPs that integrate X-ray activated NIR persistent luminescence (PersL), high X-ray attenuation and excellent magnetic properties into a single nanoparticle (NP). In this case, Cr3+ is used as the luminescence center. The co-doped Yb3+ and coating effectively enhance the X-ray activated NIR PersL. At the same time, the presence of the high-Z element Ta also makes the GGTO NPs exhibit high X-ray attenuation performance, which can be used as a CT contrast agent to achieve in vivo CT imaging. In addition, since the matrix contain a large amount of Gd, the GGTO NPs show remarkable magnetic properties, which can realize in vivo MR imaging. GGTO NPs combine the trimodal benefits of X-ray reactivated PersL, CT and MR imaging and are suitable for single or combined applications that require high sensitivity and spatial resolution imaging
研究领域:
材料科学
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生物医学工程
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成像科学
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纳米技术
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PubMed
高影响因子
EUROPEAN JOURNAL OF MEDICAL RESEARCH
·
2024
·Vol. 29
·期 1
·1-14
·
IF 2.8
Zhang Peng,Liu Jie,Chai Zijia,Fu Jinjin,Li Shuwen,Yang Zhe
Background Circular RNAs (circRNAs) are involved in the progression of osteoporosis; however, their impact on osteogenic differentiation has yet to be fully elucidated. In this study, we identified a novel circRNA known as circZfp644-205 and investigated its effect on osteogenic differentiation and apoptosis in osteoporosis. Methods CircZfp644-205, miR-445-3p, and SMAD2 levels were measured using quantitative real-time polymerase chain reaction (qRT-PCR). MC3T3-E1 cells were subjected to microgravity (MG) to establish a cell model. Osteogenic differentiation was assessed using qRT-PCR, Alizarin Red S staining, alkaline phosphatase staining, and western blot. The apoptosis was evaluated using flow cytometry. The relationship between miR-445-3p and circZfp644-205 or SMAD2 was determined using bioinformatics, RNA pull-down, and luciferase reporter assay. Moreover, a hindlimb unloading mouse model was generated to investigate the role of circZfp644-205 in vivo using Micro-CT. Results CircZfp644-205 expression was up-regulated significantly in HG-treated MC3T3-E1 cells. Further in vitro studies confirmed that circZfp644-205 knockdown inhibited the osteogenic differentiation and induced apoptosis of pre-osteoblasts. CircZfp644-205 acted as a sponge for miR-455-3p, which reversed the effects of circZfp644-205 on pre-osteoblasts. Moreover, miR-455-3p directly targeted SMAD2, thus inhibiting the expression of SMAD2 to regulate cellular behaviors. Moreover, circZfp644-205 alleviated the progression of osteoporosis in mice. Conclusions This study provides a novel circRNA that may serve as a potential therapeutic target for osteoporosis and expands our understanding of the molecular mechanism underlying the progression of osteoporosis.
研究领域:
分子生物学
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细胞生物学
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骨质疏松症研究
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生物医学工程
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PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2019
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IF 8.355
Yang Xuan,Ruo-Yun Zhang,Dong-Hui Zhao,Xiao-Shuai Zhang,Jie An,Kai Cheng,Xiao-Lin Hou,Xian-Lin Song,Yuan-Di Zhao,Xiao-Quan Yang
In this work, simple raw materials and reaction conditions were used to synthesize a versatile nanoprobe using a one-step method. Graphene quantum dot (GQD) and gold chloride were mixed and irradiated with ultra-violet (UV) radiation for 1 min. Then, the gold nanosphere cluster with the diameter of 50 nm and coated using GQD was formed using a facile one-step approach. GQD played the roles of reducing agent, stabilizer and drug carrier instead of a harmful reducing agent or stabilizer. The nanoprobe had good dispersion, stability, excellent photoacoustic imaging (PAI) and computed tomography (CT) imaging performance, low cytotoxicity and photothermal conversion efficiency of up to 51.31%. The results for cell and animal experiments showed that targeted PAI/CT imaging of tumor after modification of folic acid (FA) could be obtained using the probe. Meanwhile, after the adsorption of doxorubicin, the chemo-photothermal combined therapy for tumor could be carried out through controlled drug release from GQD under heated and acidic environment of tumor. Additionally, the treatment effect was significantly superior to single modes. The body weight, Hematoxylin and Eeosin (H&E) staining of main organs and blood biochemical indicators showed that the probe had good biological safety after injection. The current work proposes a new dual-mode bio-imaging and chemo-photothermal combined therapy nanoprobe, which presents good application prospect for tumor theragnostic.
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JOURNAL OF CONTROLLED RELEASE
·
2024
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IF 10.5
Xuan Meng,Ning Zhao,Delong Zhao,Huanyu Zhao,Mengjiao Wang,Tianyang Zhao,Shuli Man,Yujie Dai,Yanjun Zhao
Sonodynamic anti-cancer therapy relies on the highly active singlet oxygen to induce potent cell death. However, the non-specific biodistribution of sonosensitizers post systemic administration results in a significant accumulation in the skin, and hence the daylight-induced phototoxicity. Here, we report a smart metal-organic framework-based nanocarrier with titanium dioxide (TiO 2 ) as the sonosensitizer for reduced phototoxicity in the skin. The organic ligand bears the imidazole moiety that can facilely consume singlet oxygen in the skin without compromising the anti-cancer efficacy. The reaction between imidazole moiety and singlet oxygen was confirmed by the density functional theory (DFT). Upon light irradiation, the nanocarrier can significantly reduce the phototoxicity post light irradiation in a range of normal cells in vitro and in a mouse model in vivo . Meanwhile, the ligand contains a disulfide moiety that can deplete glutathione and orchestrate the singlet oxygen-induced toxicity in the CT-26 colon cancer cells. As a result, the nanocarrier showed superior in vivo antitumor efficacy in a CT-26 tumor-bearing mice model, leading to significant suppression of tumor growth and improved animal survival rates. The current work provides a tailored nanoscale particle engineering approach to simultaneously minimize phototoxicity in the skin and sensitize sonodynamic anti-cancer therapy.
研究领域:
癌症治疗
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纳米技术
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材料科学
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生物医学工程
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PubMed
高影响因子
Scientific Reports
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2024
·Vol. 14
·期 1
·1-14
·
IF 3.8
He Keting,Wei Dongfan,Liu Qiuhong,Liu Xiaoli,Zhou Diwenxin,Chen Shangci,Zhu Danhua,Xu Xiaowei
Acute liver injury (ALI) presents a challenging problem worldwide, prompting extensive research efforts. Cellular senescence has been found to be induced following ALI, and targeting cellular senescence has shown therapeutic potential. Therefore, understanding the expression of senescence-related genes in ALI can help to explore pathogenesis and treatment of this common disease. Carbon tetrachloride (CCl 4 ) is commonly used to study ALI. Although polymerase chain reaction (PCR) is a convenient and economical molecular biology technique widely used in basic medicine, research on selecting suitable reference genes to obtain objective and reproducible PCR data is scarce. Moreover, evidence supporting the choice of reference genes for experimental studies of CCl4-induced ALI and hepatic senescence in mice is limited. In this study, we obtained murine livers at four time points (0, 12, 24, and 48 h) following CCl4 treatment. We used five algorithms (geNorm, BestKeeper, NormFinder, delta Ct, and RefFinder) to rank 12 candidate genes in real-time reverse-transcription quantitative PCR (RT-qPCR) experiments. Focusing on cellular senescence in this model, we adopted four senescence-associated secretory phenotype (SASP) genes ( Il6 , Il1b , Ccl2 , and Ccl5 ) as target genes. Our results confirmed Gapdh and Tbp as suitable reference genes in murine CCl 4 -induced ALI models. Furthermore, we provide a table of published studies recommending reference genes for various liver disease models. This study provides a valuable reference for enhancing the reliability and reproducibility of ALI molecular findings.
研究领域:
分子生物学
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肝病学
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病理学
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基因组学
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PubMed
高影响因子
Bioactive Materials
·
2021
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IF 14.593
Xiaohui Wu,Xin Wang,Xiaobin Chen,Xiaowei Yang,Qian Ma,Guohua Xu,Lin Yu,Jiandong Ding
It is very challenging to visualize implantable medical devices made of biodegradable polymers in deep tissues. Herein, we designed a novel macromolecular contrast agent with ultrahigh radiopacity (iodinate content > 50%) via polymerizing an iodinated trimethylene carbonate monomer into the two ends of poly(ethylene glycol) (PEG). A set of thermosensitive and biodegradable polyester-PEG-polyester triblock copolymers with varied polyester compositions synthesized by us, which were soluble in water at room temperature and could spontaneously form hydrogels at body temperature, were selected as the demonstration materials. The addition of macromolecular contrast agent did not obviously compromise the injectability and thermogelation properties of polymeric hydrogels, but conferred them with excellent X-ray opacity, enabling visualization of the hydrogels at clinically relevant depths through X-ray fluoroscopy or Micro-CT. In a mouse model, the 3D morphology of the radiopaque hydrogels after injection into different target sites was visible using Micro-CT imaging, and their injection volume could be accurately obtained. Furthermore, the subcutaneous degradation process of a radiopaque hydrogel could be non-invasively monitored in a real-time and quantitative manner. In particular, the corrected degradation curve based on Micro-CT imaging well matched with the degradation profile of virgin polymer hydrogel determined by the gravimetric method. These findings indicate that the macromolecular contrast agent has good universality for the construction of various radiopaque polymer hydrogels, and can nondestructively trace and quantify their degradation in vivo . Meanwhile, the present methodology developed by us affords a platform technology for deep tissue imaging of polymeric materials.
研究领域:
材料科学
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生物医学工程
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成像技术
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聚合物化学
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DOI
PubMed
高影响因子
ACS Nano
·
2024
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IF 15.8
Xuantong Zhou,Fene Gao,Wenyan Gao,Qingzhen Wang,Xin Li,Xinyue Li,Wenxin Li,Jing Liu,Huige Zhou,Aiping Luo,Chunying Chen,Zhihua Liu
Despite the superior efficacy of radiotherapy in esophageal squamous cell carcinoma (ESCC), radioresistance by cancer stem cells (CSCs) leads to recurrence, metastasis, and treatment failure. Therefore, it is necessary to develop CSC-based therapies to enhance radiotherapy. miR-339-5p (miR339) is involved in stem cell division and DNA damage checkpoint signaling pathways based on ESCC cohort. miR339 inhibited ESCC cell stemness and enhanced radiation-induced DNA damage by targeting USP8, suggesting that it acts as a potential CSC regulator and radiosensitizer. Considering the limited circulating periods and poor tumor-targeting ability of miRNA, a multifunctional nanoplatform based on bismuth sulfide nanoflower (Bi@PP) is developed to efficiently deliver miR339 and improve radioresistance. Intriguingly, Bi@PP encapsulates more miR339 owing to their flower-shaped structure, delivering more than 1000-fold miR339 into cells, superior to free miR339 alone. Besides being used as a carrier, Bi@PP is advantageous for dynamically monitoring the distribution of delivered miR339 in vivo while simultaneously inhibiting tumor growth. Additionally, Bi@PP/miR339 can significantly enhance radiotherapy efficacy in patient-derived xenograft models. This multifunctional platform, incorporating higher miRNA loading capacity, pH responsiveness, hypoxia relief, and CT imaging, provides another method to promote radiosensitivity and optimize ESCC treatment.
研究领域:
肿瘤学
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分子生物学
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纳米技术
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放射生物学
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治疗学
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PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Xinglu Jiang,Kangjing Li,Beibei Xie,Jun Zhou
Taq DNA polymerase is the most widely used enzyme for PCR amplification and it contains 832 amino acids but no cysteines. Bovine serum albumin (BSA) is an 832-amino acid protein containing 35 cysteines, and at high temperature BSA can produce protein hydrogels through recombined disulfides and noncovalent bonds. When hydrogels are prepared using a mixture of Taq DNA polymerase and BSA, no disulfide bonds occur between them, and noncovalent bonds are the main forces holding together the Taq DNA polymerase with the hydrogels. During PCR amplification, temperature cycling can destroy the noncovalent bonds, thereby releasing Taq DNA polymerase from the hydrogels to synthesize DNA. The release rate, regulated through the variation of BSA concentration, allows for velocity-controlled amplification of DNA. As the amount of DNA products exceeds that of the Taq DNA polymerase released from hydrogels, a linear amplification replaces the exponential PCR amplification. When the amplification velocity begins to decrease, the time needed for reaching a plateau increase. This provides a broader detection window for the analysis of the end products, which correspond to the difference compared with the initial DNA concentration. The number of primers is continuously reduced with increasing cycles, which further causes a decline in the DNA amplification efficiency. The difference in the initial DNA template may cause a different consumption of primers, which can cause errors in the Ct values in the case of qPCR. Based on the slopes of the linear amplification curve, the Ct values can be calibrated to reduce this error, and a more accurate detection of the initial content of the target DNA can be implemented.
研究领域:
分子生物学
·
生物技术
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生物化学
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高影响因子
BIOMATERIALS
·
2012
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IF 7.404
Zhen Liu,Fang Pu,Sa Huang,Qinghai Yuan,Jinsong Ren,Xiaogang Qu
Due to their unique electric, magnetic, and optical properties, engineered nanostructures have been applied to provide diagnostic, therapeutic, as well as prognostic information about the status of disease. In this study, we report a multifunctional nanoprobe based on PEGylated Gd 2 O 3 :Yb 3+ , Er 3+ nanorods (denoted as PEG-UCNPs) for in vivo up-conversion luminescence (UCL), T 1 -enhanced magnetic resonance (MR), and X-ray computed tomography (CT) multi-modality imaging. A facile and large-scale hydrothermal system combining the merits of an in situ thermal decomposition method and a surface-modified approach is introduced to construct high-quality PEG-UCNPs. By grafting PEG molecules on the surface of PEG-UCNPs, the nanostructures possess excellent stability against in vivo environment and hold long blood circulation time. Cell-cytotoxicity assay, hemolyticity, as well as post-injection histology, hematology, and inflammation analysis further demonstrate their non-cytotoxic character and indicate further in vivo application. In detail, the capability of PEG-UCNPs as high-performance contrast agents for UCL/MR/CT imaging is evaluated successfully through small-animal experiments. Additionally, pharmacokinetics, biodistribution, and clearance route are studied after intravenous injection in a mouse model, reflecting their overall safety.
研究领域:
纳米技术
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材料科学
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生物医学工程
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医学影像
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分子诊断
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）