APPLIED CATALYSIS B-ENVIRONMENTAL
·
2017
·
IF 9.446
Yuyang Liu,Xuemin Liu,Yaping Zhao,Dionysios D. Dionysiou
The self-assembled synthesis of a hierarchical graphene oxide (GO)-carbon nanotubes (CNTs)-α-FeOOH decorated composite aerogel (α-FeOOH@GCA) through a facile in-situ hydrolysis route is reported for the first time and the materials was tested for its performance as Fenton-like catalyst. The introduction of GO-CNTs clearly mediated the morphology to aligned α-FeOOH nanorods ( ca. 100 nm) within aerogel matrix comparing with pristine urchin-like α-FeOOH three-dimensional microstructures ( ca. 1 μm). This three dimensional porous aerogel network provided efficient charge/mass-transfer leading to great enhancement of the catalytic activity of α-FeOOH. The outstanding catalytic performance of this composite in degradation of organics with different charge and structure, i.e. Orange II (OII), rhodamine B (RhB), methylene blue (MB), phenol and endocrine disruptor bisphenol A (BPA) was demonstrated. For example, the discoloration of OII with pseudo first-order rate constant of 0.10 min −1 significantly exceeded that of pristine α-FeOOH. At relatively low concentration of α-FeOOH@GCA catalyst (125 mg L −1 ) and H 2 O 2 (0.55 mM) showed excellent catalytic activity for efficient (∼99%) discoloration of OII (40 mg L −1 ) under a 60 min UV 365 irradiation in the pH range 3–10. The different charge of five target contaminats greatly determined the surface-catalyzed degradation kinetics at natural solution pH in the order of cationic > neutral > anionic organics due to the negatively charged carbon-based aerogel matrix. The new identified desulfonation intermediates elucidated through UPLC–MS analysis indicated two reaction pathways: 1) hydroxylation and 2) desulfonation by-products followed by cleavage of the azo bond as the predominant degradation pathway of OII. The elimination of the acute toxicity of the parent contaminant to luminescent bacterium Q67 was consistent with the formation of less toxic degradation by-products identified. Free radical quenching studies accessed the role of hydroxyl radical ( OH), superoxide anion radical (O 2 − ) and singlet oxygen ( 1 O 2 ) as the dominating reactive oxygen species (ROS). The quantitative studies to measure radical concentrations using relative molecular probes showed the effective activation of H 2 O 2 led to high rate production of ROS accounted for OII degradation. The greatly enhanced photocatalytic property of this hybrid was correlated with the efficient conversion between Fe 2+ /Fe 3+ and synergistic coupling between α-FeOOH and carbon-based aerogel matrix evidenced through the formation of Fe O C chemical bonds was verified by X-ray photoelectron (XPS) analysis. Based on its simple and scalable preparation route as well as its excellent UV 365 or visible light-responsive catalytic performance, this hybrid exhibits a high potential to be used as an efficient and environmental-friendly catalyst for water remediation.
研究领域:
环境科学
·
材料科学
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DOI
高影响因子
ACS Biomaterials Science & Engineering
·
2017
·
IF 3.234
Feng Li,Tianyu Li,Xuexiang Han,Hao Zhuang,Guangjun Nie,Huaping Xu
Selenium is a unique, essential trace element that plays an important role in the antioxidant defense and redox regulation of biological processes. We have reported that novel selenium-containing platinum-based anticancer molecules (EG-Se/Pt) had selective cytotoxicity toward cancer cells. Herein, we found the underlying mechanism of selective cytotoxicity to be closely related to the glutathione antioxidant defense system. Elevated reactive oxygen species (ROS) make cancer cells more vulnerable to further elevation of ROS. EG-Se/Pt can induce abnormal increases in ROS by depletion of glutathione. Consequently, the mitochondrial membrane potential collapses and cytochrome c is released, resulting in cell apoptosis. However, EG-Se/Pt analogues, such as EG-Se/Cu and EG-Se/Ni, did not exhibit glutathione depletion capacity or selective killing activity in our investigation, although they can effectively kill cancer cells. These results suggest that the glutathione antioxidant system is an effective target to enable therapeutic selectivity. The amphiphilic property of the selenium–platinum coordination molecules facilitates their assembly into nanoparticles and prolongs the circulation time of the drug in the bloodstream, which is important for in vivo drug delivery. Our in vivo anticancer study demonstrated that the tumor growth inhibition rate of EG-Se/Pt can reach 69% (p < 0.05). What is more encouraging is that EG-Se/Pt exhibited minimal side effects compared to cisplatin. This work also provides new opportunities for the development of therapeutic strategies against cancer.
研究领域:
肿瘤学
·
药理学
·
生物化学
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2023
·
IF 4.4
Ciyang Zhang,Keren Lu,Linrui Li,Wu Lei,Mingzhu Xia,Fengyun Wang
Oxytetracycline hydrochloride (OTC) is a commonly used over-the-counter antibiotic, valued for its potent antibacterial properties. However, the inappropriate and excessive use of OTC can result in the accumulation of the drug in both the environment and human body, causing significant harm to ecosystems and human health. Therefore, the development of a fast and sensitive method for detecting OTC is of great significance. Lanthanide metal–organic frameworks (LnMOFs) can effectively excite lanthanide metals to emit long-lifetime, narrow and stable fluorescence based on the antenna effect, but their application in fluorescence sensing is rarely reported. In this work, a strongly fluorescent material Tb-MOF was synthesized by a facile solvothermal method using 1,2,4,5-phenylenetetracarboxylic acid (H 4 btec) and 1,10-phenanthroline (1,10-phen) as organic ligands and lanthanide metal Tb as the luminescent center. Tb-MOF is a stable material in water and shows excellent linearity with OTC in the concentration range of 0 ∼ 70 μM, with low detection limit (0.12 μM) and luminescence color transition from bright green to dark green during the detection process. X-ray diffraction, UV–vis absorption and fluorescence lifetime analyses revealed that the fluorescence quenching of Tb-MOF by OTC is caused by the inner filter effect in static quenching. Test strips for OTC detection were successfully prepared using Tb-MOF. These strips are not only low cost and easy to prepare but can also be used as portable sensing devices that can be easily distinguished by the naked eye during OTC testing. This study not only presents a fluorescent probe for the detection of OTC in water but also offers a practical method for converting fluorescent luminescent materials into functional devices for OTC detection.
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PubMed
高影响因子
JOURNAL OF CONTROLLED RELEASE
·
2023
·
IF 10.8
Xin Yang,Wanqiong Li,Shuzhen Li,Shaomeng Chen,Zheng Hu,Zhuoying He,Xueqin Zhu,Xiaoshuang Niu,Xiuman Zhou,Huihao Li,Youmei Xiao,Juan Liu,Xinghua Sui,Guanyu Chen,Yanfeng Gao
Peptide immune checkpoint inhibitors in cancer immunotherapy have attracted great attention recently, but oral delivery of these peptides remains a huge challenge due to the harsh gastrointestinal environment, large molecular size, high hydrophilic, and poor transmembrane permeability. Here, for the first time, a fish oil-based microemulsion was developed for oral delivery of programmed death-1/programmed cell death-ligand 1 (PD-1/PD-L1) blocking model peptide, OPBP-1. The delivery system was characterized, in vitro and in vivo studies were conducted to evaluate its overall implication. As a result, this nutraceutical microemulsion was easily formed without the need of co-surfactants, and it appeared light yellow, transparent, good flowability with a particle size of 152 ± 0.73 nm, with a sustained drug release manner of 56.45 ± 0.36% over 24 h and a great stability within the harsh intestinal environment. It enhanced intestinal drug uptake and transportation over human intestinal epithelial Caco-2 cells, and drastically elevated the oral peptide bioavailability of 4.1-fold higher than that of OPBP-1 solution. Meanwhile, the mechanism of these dietary droplets permeated over the intestinal enterocytic membrane was found via clathrin and caveolae-mediated endocytic pathways. From the in vivo studies, the microemulsion facilitated the infiltration of CD8 + T lymphocytes in tumors, with increased interferon-γ (IFN-γ) secretion. Thus, it manifested a promising immune anti-tumor effect and significantly inhibited the growth of murine colonic carcinoma (CT26). Furthermore, it was found that the fish oil could induce ferroptosis in tumor cells and exhibited synergistic effect with OPBP-1 for cancer immunotherapy. In conclusion, this fish oil-based formulation demonstrated great potential for oral delivery of peptides with its natural property in reactive oxygen species (ROS)-related ferroptosis of tumor cells, which provides a great platform for functional green oral delivery system in cancer immunotherapy.
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DOI
PubMed
高影响因子
Oncotarget
·
2015
·
IF 6.359
Liyuan Wan,Haiyan Dong,Huo Xu,Ji Ma,Yewei Zhu,Yusheng Lu,Jichuang Wang,Ting Zhang,Tao Li,Jingjing Xie,Bo Xu,Fangwei Xie,Yu Gao,Jingwei Shao,Xiaohuang Tu,Lee Jia
Recent scientific advances have increased our understanding of the cancer metastatic complexities and provided further impetus for new combination therapies to prevent cancer metastasis. Here, we demonstrated that a combination (HAMPT) of aspirin, lysine, mifepristone and doxycycline can effectively and safely prevent cancer metastasis. The pharmaceutically-formulated HAMPT inhibited adhesion of cancer cells to either endothelial cells or extracellular matrix via down-regulating cell adhesion molecules ICAM-1 and α 4 -integrin. HAMPT inhibited the cloak effect by activated platelets on cancer cells, thereby interfering adhesion and invasion of cancer cells to the underlying stroma. At the effective concentration, HAMPT induced cancer cells into dormancy with minor inhibition on cell viability. Four-day pretreatment followed by 30-day oral administration of HAMPT (33.5-134 mg/kg) to the mice inoculated with cancer cells produced significant inhibition on cancer metastasis dose-dependently without marked side effects. Fifty-day rat toxicity study with HAMPT at doses (335-1340 mg/kg) 20-fold higher than its therapeutic dose produced no significant toxicity. Interestingly, the acute toxic death could not be reached at the maximum administrable dose (5 g/kg). This proof-of-concept study provides the first conceptual evidence that cancer metastasis can be controlled by using affordable old drugs to restrain circulating tumor cells from gemmating on the metastatic soil without the need for cytotoxicity.
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PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Jinyang Chen,Li Gan,Yonghe Han,Gary Owens,Zuliang Chen
Ferrous sulfide nanoparticles ( n FeS) have proven to be effective in removing heavy metals (HMs) from wastewater. One such approach, which has garnered much attention as a sustainable technology, is via the in situ microbial synthesis of n FeS. Here, a sulfate-reducing bacteria (SRB) strain, Geobacter sulfurreducens , was used to initially biosynthesize ferrous sulfide nanoparticles (SRB- n FeS) and thereafter remove HMs from acid mine drainage (AMD). SRB-nFeS was characterized by X-ray powder diffraction (XRD), scanning electron microscopy (SEM) coupled to an energy dispersive spectrometer (EDS), three-dimensional excitation-emission matrix (3D-EEM) spectroscopy, Fourier transform infrared (FTIR) spectroscopy and X-ray photoelectron spectroscopy (XPS). Such characterization showed that SRB mediated the reduction of SO 4 2- to S 2- to form n FeS, where the metabolized substances functioned as complexing agents which coordinated with n FeS to form biofunctional SRB- n FeS with improved stability. One advantage of this synthetic route was that the attachment of n FeS to the bacterial surface protected SRB cells from HM toxicity. Furthermore, due to a synergistic effect between n FeS and SRB, HM removal from both solution and AMD by SRB- n FeS was enhanced relative to the constituent components. Thus, after 5 consecutive cycles of HM removal, SRB- n FeS removed, Pb(Ⅱ) (92.6%), Cd(Ⅱ) (78.7%), Cu(Ⅱ) (76.0%), Ni(Ⅱ) (62.5%), Mn(Ⅱ) (62.2%), and Zn(Ⅱ) (88.5%) from AMD This study thus provides new insights into the biosynthesis of SRB- n FeS and its subsequent practical application in the removal of HMs from AMD.
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产品关联
DOI
PubMed
高影响因子
Drug Design Development and Therapy
·
2022
·
IF 4.319
Jin Wang,Ye Qian,Xuemin Gao,Na Mao,Yucong Geng,Gaojie Lin,Guibin Zhang,Han Li,Fang Yang,Hong Xu
Background N-Acetyl-seryl-aspartyl-lysyl-proline (Ac-SDKP) is a short peptide with an anti-silicosis effect. However, the short biological half-life and low plasma concentration of Ac-SDKP hamper discovery of specific targets in organisms and reduce the anti-silicosis effect. A novel peptide, Ac-SDK (biotin) proline, termed “Ac-B”, with anti-fibrotic properties was synthesized.Methods Ac-B was detected quantitatively by high-performance liquid chromatography. Phagocytosis of Ac-B by the alveolar epithelial cell line A549 was investigated by confocal laser scanning microscopy and flow cytometry. To further elucidate the cellular-uptake mechanism of Ac-B, chemical inhibitors of specific uptake pathways were used. After stimulation with transforming growth factor-β1, the effects of Ac-B on expression of the myofibroblast marker vimentin and accumulation of collagen type I in A549 cells were analyzed by Western blotting. Sirius Red staining and immunohistochemical analyses of the effect of Ac-B on expression of α-smooth muscle actin (SMA) in a rat model of silicosis were undertaken.Results Ac-B had good traceability during the uptake, entry, and distribution in cells. Ac-B treatment prevented an increase in α-SMA expression in vivo and in vitro and was superior to that of Ac-SDKP. Caveolae-mediated uptake of Ac-B by A549 cells led to achieving anti-epithelial–mesenchymal transformation (EMT) effects.Conclusion Ac-B had an anti-fibrotic effect and could be a promising agent for the fibrosis observed in silicosis in the future.
研究领域:
药理学
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肺科学
·
细胞生物学
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DOI
PubMed
高影响因子
RSC Advances
·
2017
·Vol. 7
·期 27
·16730-16736
·
IF 3.108
Ruiqing Feng,Longlong Li,Bing Li,Jinhui Li,Dan Peng,Yitao Yu,Qiuhong Mu,Ning Zhao,Xiaoqiang Yu,Zhenhua Wang
We have synthesized two-photon organic molecule probes (IMT-E and IMT-M), which selectively stain endogenous RNA in the nucleolus and cytoplasm of living cells in a short incubation time. Using these probes, bright two-photon excitation fluorescence (TPEF) images of living cells have been developed. Titrations and digest tests of ribonuclease indicate the markedly higher affinity of these probes for RNA, especially when using IMT-E. Upon binding to RNA, the fluorescence intensity of IMT-E increases by about 15-fold, showing that IMT-E is a turn-on probe for the detection of RNAs. MTT assays demonstrate that the mitochondria of cells maintain their electron mediating ability after being stained with the probes. These results demonstrate that IMT-E is an attractive two-photon turn-on fluorescent probe for visualizing RNA in living cells.
研究领域:
生物化学
·
分子生物学
·
细胞生物学
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DOI
高影响因子
Journal of Biomedical Nanotechnology
·
2022
·Vol. 18
·期 12
·2818-2827
·
IF 3.641
Yang You,Wang Xing-Heng,Wang Jun,Zhang Ju-Ying,Chen Wen,Yang Hao,He Ping,Tang Xiao-Qing,Yu Jin-Hong
Nanoscale phase-variant ultrasound contrast agents have attracted the interest of many researchers. However, it is a challenge to design nanobubbles that are activated by low-frequency medical ultrasound that do not cause damage to normal tissues. In this study, we designed a new type
of nanoparticle consisting of perfluoropentane and iron polyphthalocyanine loaded into liposomes. These nanoparticles can be activated by a low-frequency medical ultrasound imager at a frequency of 5 MHz for ultrasound imaging and clear reactive oxygen species at a rate of more than 50%. This
ability to scavenge excess reactive oxygen species can alleviate the damage these species cause and protect macrophages. Moreover, these nanoparticles can effectively enhance ultrasound contrast imaging for real-time visualization in the diagnosis and treatment of diseases.
研究领域:
医学影像学
·
纳米医学
·
生物化学
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DOI
高影响因子
BRITISH JOURNAL OF CANCER
·
2022
·Vol. 128
·期 2
·219-231
·
IF 9.075
Yang Bingxue,Xie Xinran,Lv Dazhao,Hu Jiajun,Chen Yuyun,Wu Zhaoyu,Luo Shuyue,Zhang Shiyi
Background Hand-foot syndrome (HFS) is a serious dose-limiting cutaneous toxicity of capecitabine-containing chemotherapy, leading to a deteriorated quality of life and negative impacts on chemotherapy treatment. The symptoms of HFS have been widely reported, but the precise molecular and cellular mechanisms remain unknown. The metabolic enzyme of capecitabine, thymidine phosphorylase (TP) may be related to HFS. Here, we investigated whether TP contributes to the HFS and the molecular basis of cellular toxicity of capecitabine. Methods TP-/- mice were generated to assess the relevance of TP and HFS. Cellular toxicity and signalling mechanisms were assessed by in vitro and in vivo experiments. Results TP-/- significantly reduced capecitabine-induced HFS, indicating that the activity of TP plays a critical role in the development of HFS. Further investigations into the cellular mechanisms revealed that the cytotoxicity of the active metabolite of capecitabine, 5-DFUR, was attributed to the cleavage of GSDME-mediated pyroptosis. Finally, we demonstrated that capecitabine-induced HFS could be reversed by local application of the TP inhibitor tipiracil. Conclusion Our findings reveal that the presence of elevated TP expression in the palm and sole aggravates local cell cytotoxicity, further explaining the molecular basis underlying 5-DFUR-induced cellular toxicity and providing a promising approach to the therapeutic management of HFS.
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PubMed
高影响因子
APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING
·
2022
·Vol. 128
·期 8
·1-11
·
IF 2.983
Chen Zhangxu,Zhu Danchen,Zhang Lidan
To overcome the intrinsic disadvantages of pristine g-C 3 N 4 and make it reusable, improve the activity of g-C 3 N 4 by making use of the near-infrared region, the magnetic Fe 3 O 4 /β-NaYF 4 /g-C 3 N 4 (FO/UC/CN) heterogeneous materials with high and stable NIR enhanced photocatalytic activity were prepared via a new facile one-step solvothermal synthesis method. It can be revealed that FO/UC/CN can activate UV–visible light under excitation of 980 nm. Compared with pure Fe 3 O 4 , g-C 3 N 4 and Fe 3 O 4 /g-C 3 N 4 , the obtained FO/UC/CN15 heterojunction exhibits good photodegradation rates of 65.56, 21.35, 59.22 and 20.49% for MB, MO, RhB and phenol, respectively. In addition, after four cycles of the photocatalytic degradation test, the photodegradation rates remain 62.14, 20.54, 57.64 and 19.36%, respectively. The results illustrate magnetic FO/UC/CN heterojunction exhibits highly NIR-enhanced photocatalytic activity and excellent stability. This study indicates a promising system to synthesize g-C 3 N 4 -based magnetic photocatalysis for effectively utilizing the NIR and treating industrial wastewater, which can be expected to alleviate the energy crisis and enhance the application of solar energy for dealing with environmental remediation in the future.
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高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2020
·
IF 10.652
Na Li,Huai-duo Chen,Yong-ze Lu,Ming-chao Zhu,Zhao-xia Hu,Shou-wen Chen,Raymond Jianxiong Zeng
In recent years, nanoscale zero-valent iron (nZVI) has been considered an appealing alternative for halogenated organic compound removal for its advantages, such as its high activity. However, easy aggregation limits nZVI activity. In this study, functional nZVI-modified PVDF membranes (nZVI@PVDF membranes) were successfully prepared by a simple and easy-to-follow filter-press coating method and was applied in the ultrafiltration process to treat 2-chlorophenol (2-cp) wastewater. Scanning electron microscopy showed that nZVI particles were relatively uniformly distributed on the membrane surface and formed a fluffy and porous space-stacked structure. 2-cp ultrafiltration degradation results illustrated that nZVI@PVDF membranes revealed good 2-cp removal efficiencies ranged from 56.06% to74.62%. These 2-cp removal efficiencies were higher than those for Fe supported on powder materials and were relatively comparable to those for nZVI composite membranes prepared by complex methods (e.g., blend spinning and cross-linking methods). Furthermore, the 2-cp degradation efficiencies by nZVI@PVDF membranes were not affected much in the presence of natural organic matter and different ions, demonstrating a robust anti-interference ability of nZVI@PVDF membrane. The mechanism underlying its robust performance likely resulted from that nZVI was uniformly distributed and loaded layer by layer on the PVDF membrane surface with high loading capacity, which guaranteed high nZVI activity, layer-layer interception of pollutants and complete reaction between nZVI and pollutants. These findings provide key insights into how nZVI@PVDF membranes could be integrated into the treatment of wastewater containing refractory pollutants.
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高影响因子
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