TALANTA
·
2019
·
IF 4.916
Guoliang Liu,Da-Qian Feng,Yanling Qian,Wei Wang,Jun-Jie Zhu
Exploring new energy donor/acceptor pairs for constructing FRET system has important research significance. In this study, a FRET assembly by using carbon dots (CDs) as energy donor and gold nanorods (Au NRs) as energy acceptor has been proposed through covalent bond interactions by taking advantage of cysteamine as a bridge. The assembly is characterized by UV–vis absorption spectra, fluorescence spectra , FT-IR spectra and TEM images, revealing that Au NRs successfully absorbed on the surface of CDs. As for the occurrence of FRET process from CDs to Au NRs, the fluorescence signal of CDs-cysteamine-Au NRs assembly quenched significantly. Interestingly, Pb 2+ ions could bind completely with cysteamine and disturbed the FRET process, which activated fluorescence signal of the system. Based on these experimental phenomena, CDs-cysteamine-Au NRs assembly was performed as an off-on FRET biosensor for Pb 2+ ions. The linear range obtained is from 0 to 155 μM with the detection limit of 0.05 μM. Moreover, the FRET-based sensor exhibited high selectivity to the analyte Pb 2+ ions against other interference substances. Furthermore, the present approach was successfully applied to detect Pb 2+ ions in real samples, which suggested its potential applications in environmental monitoring.
研究领域:
分析化学
·
环境科学
✓ 已验证
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2016
·
IF 4.712
Cuiling Zhang,Caiping Ding,Guohua Zhou,Qin Xue,Yuezhong Xian
DNA functionalized quantum dots (QDs) are promising nanoprobes for the fluorescence resonance energy transfer (FRET)-based biosensing. Herein, cadmium-free DNA functionalized Mn-doped ZnS (DNA-ZnS:Mn 2+ ) QDs were successfully synthesized by one-step route. As-synthesized QDs show excellent photo-stability with the help of PAA and DNA. Then, we constructed a novel FRET model based on the QDs and WS 2 nanosheets as the energy donor-acceptor pairs, which was successfully applied for the protein detection through the terminal protection of small molecule-linked DNA assay. This work not only explores the potential bioapplication of the DNA-ZnS:Mn 2+ QDs, but also provides a platform for the investigation of small molecule-protein interaction.
研究领域:
纳米技术
·
生物传感
·
材料科学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
·
2024
·
IF 1.2
Yuzheng CAI,Ge GUO,Yankun FU,Xianqing HUANG,Tianlin WANG,Tiange LI
Simultaneous and rapid detection of various mycotoxins in food holds significant practical importance in the field of food processing and safety. In this study, a fluorescent aptasensor based on functionalized graphene oxide (FGO) is developed for simultaneous detection of aflatoxin B 1 (AFB 1 ) and aflatoxin M 1 (AFM 1 ). The two aptamers specific to AFB 1 and AFM 1 are labeled with Cy3 and Cy5 respectively. Both the aptamers can be adsorbed onto the surface of FGO through π - π stacking, resulting in fluorescence resonance energy transfer (FRET) between the fluorophore and FGO. The absence of target leads to quenching of fluorescence while presence of either aflatoxin causes interaction between corresponding aptamer and target, leading to release from FGO surface thereby turning on fluorescence signal. The limit of detection (LOD) for AFB 1 is determined as 8.7 pg/mL whereas for AFM 1 it is found to be 20.1 pg/mL, demonstrating fast and sensitive detection capability using this approach. Furthermore, the aptasensor exhibits good specificity and selectivity even under influence from other common interfering toxins. With its simplicity in operation and portability features, this sensor has potential applications for establishing sensitive and portable on-site detection methods for various mycotoxins.
研究领域:
食品安全
·
分析化学
·
生物传感技术
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2016
·
IF 7.476
Ye Wang,Ning Gan,You Zhou,Tianhua Li,Yuting Cao,Yinji Chen
Herein, a smart single-stranded DNA binding protein (SSB)-assisted fluorescence aptamer switch based on fluorescence resonance energy transfer (FRET) was designed. The FRET switch was synthesized by connecting SSB labeled quantum dots ( [email protected] ) as donor with aptamer (apt) labeled gold nanoparticles ( [email protected] ) as acceptor, and it was employed for detecting chloramphenicol (CAP) in a homogenous solution. In the assay, the interaction between core-shell [email protected] and [email protected] leads to a dramatic quenching (turning off). After adding CAP in the detection system, [email protected] can bind the target specifically then separate [email protected] with [email protected] , resulting in restoring the fluorescence intensity of QDs (turning on). Consequently, the fluorescence intensity recovers and the recovery extent can be used for detection of CAP in homogenous phase via optical responses. Under optimal conditions, the fluorescence intensity increased linearly with increasing concentrations of CAP from 0.005 to 100 ng mL −1 . The limit of this fluorescence aptamer switch was around 3 pg mL −1 for CAP detection. When the analyte is changed, the assay can be applied to detect other targets only by changing relative aptamer in [email protected] probe. Furthermore, it has potential to be served as a simple, sensitive and portable platform for antibiotic contaminants detection in biological and environmental samples.
研究领域:
生物化学
·
分析化学
·
纳米生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2019
·
IF 9.518
Lin Liu,Hua Zhang,Zhenxin Wang,Daqian Song
In the present work, peptide-functionalized upconversion@silica nanoparticle (termed as UCNP@SiO 2 @Cy5-pep)-based fluorescence resonance energy transfer (FRET) sensing platform has been fabricated for the detection of caspase-9 activity in vitro and in vivo. A Cy5 labeled peptide containing specific motif LEHD for caspase-9 cleavage was designed and conjugated with UCNP@SiO 2 through covalent attachment . The red upconversion luminescence (UCL) emission of UCNP@SiO 2 can be quenched by Cy5 while the green UCL emission of UCNP@SiO 2 remains undisturbed. After the cleavage of LEHD by caspase-9, the Cy5 departed from UCNP@SiO 2 surface, resulting in recovery of red UCL emission of UCNP@SiO 2 . The UCNP@SiO 2 @Cy5-pep has been successfully used to monitoring the changes of caspase-9 activity levels in apoptotic cancerous cells (MG-63 and SW480) by cisplatin-induction. Under same experimental conditions, it is found that the intracellular caspase-9 activity level of cisplatin-treated MG-63 cells is higher than that of cisplatin-treated SW480. This result is consistent with that of commercial caspase-9 activity kits. The UCL signal intensity ratio of red emission to green emission of UCNP (termed as R/G) is linearly dependent on the amount of MG-63 cells within the range of 5 × 10 3 to 1 × 10 6 cells (i.e., 0.5–100 U mL −1 caspase-9) with a limit of detection (LOD) of 675 cells (i.e., 0.068 U mL −1 caspase-9). Furthermore, the practicability of UCNP@SiO 2 @Cy5-pep is demonstrated by detection of caspase-9 activities in tumor tissues in vivo, and satisfactory results are obtained.
研究领域:
生物化学
·
纳米医学
·
肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Feng Chen,Qiujun Lu,Youyu Zhang,Shouzhuo Yao
Due to the low abundance of miRNAs and the coordinated regulation of multiple miRNAs toward some biological processes, a method that can sensitively and simultaneously detect multiple miRNAs is needed. Here, a strand displacement dual amplification (SDDA) strategy was developed for multiple miRNAs analyses using a unique single strand-double strand-single strand DNA (sdsDNA), which generated by the target recognition probe hybridizing with the site region probe. During the process of SDDA, miRNA hybridized to the target recognition domain of sdsDNA, thereby releasing the site, which triggered the first strand displacement reaction. This strand displacement event resulted in the release of the other site, triggering the second strand displacement reaction. Both two strand displacement reactions in each assembly circle were integrated for amplified signals, which were tested by a fluorescence resonance energy transfer (FRET) assay. NaYF 4 :Yb,Tm/Er UCNPs and Ti 3 C 2 nanosheets were used as the energy donor-acceptor pair. By employing 1:2 ratio of donor-acceptor, the energy transfer efficiency between NaYF 4 :Yb,Tm/Er UCNPs and Ti 3 C 2 nanosheets could reach 90%, which enhanced signal-to-background ratio. Combining the benefits of dual amplification and efficient energy transfer on miRNAs detection, this assay exhibited a linear range from 5 fM to 100 pM for miRNA-21 and miRNA-10b. Moreover, this assay was applied to estimate the expression of miRNA-21 and miRNA-10b in human hepatoma and hepatocyte cell lines. Therefore, this FRET-based SDDA strategy has been used for highly sensitive detection of multiple miRNAs in cell lysate and facilitates the development of efficient biosensor platform.
研究领域:
生物化学
·
分子生物学
·
纳米医学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Yanbo Wang,Junjie Ma,Huan Li,Jinru Zhou,Hong Zhang,Linglin Fu
Arginine kinase (AK) is one of the most important allergens in shrimp products. Herein, a novel immunoassay for quantitation of AK was developed using the antibody modified gold nanoparticle (AuNP) and quantum dot (QD). When the first antibody modified AuNP (AuNP-Ab1) was bridged by AK with the secondary antibody modified QD (QD-Ab2), fluorescence resonance energy transfer (FRET) would occur between the AuNP and QD, which led to a decrease in fluorescent signals. The decrease in fluorescence intensity was found to correlate linearly with the log of AK concentration in the range of 1.0 × 10 -6 -1.0 × 10 -3 mg/mL (R 2 = 0.9909) and the detection limit was 0.11 ng/mL. The immunoassay was further proved to have encouraging specificity, precision and accuracy. Compared with existing methods, this study provided a promising approach to develop a highly sensitive and selective detection method for AK in shrimp related food samples.
研究领域:
分析化学
·
纳米技术
·
食品科学
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2018
·
IF 8.097
Ting Xiao,Jian Sun,Jiahui Zhao,Shuang Wang,Guoyong Liu,Xiurong Yang
As an essential and universal hydrolase, alkaline phosphatase (ALP) has been identified as a crucial indicator of various diseases. Herein, we, for the first time, expanded the application of fluorescent polydopamine (F-PDA) nanoparticles to nanoquencher-based biosensing system, as well as discovered the reversible quenching effect of manganese dioxide (MnO2) nanosheets on the fluorescence of F-PDA nanoparticles and intensively confirmed the quenching mechanism of Förster resonance energy transfer by using transmission electron microscopy, UV–vis, Fourier transform infrared spectroscopy, and fluorescence lifetime experiments. By means of the ALP-triggered generation of ascorbic acid (AA) from the substrate ascorbic acid 2-phosphate, the AA-triggered reduction of MnO2 nanosheets to Mn2+, as well as the clear quenching mechanism of F-PDA nanoparticles by MnO2 nanosheets, we have developed a label-free, low-cost, visual, and facile synthetic fluorescent biosensor for convenient assay of ALP activity. The fluorescent bioassay shows a good linear relationship from 1 to 80 mU/mL (R2 = 0.999), with a low detection limit of 0.34 mU/mL, and the excellent applicability in human serum samples demonstrates potential applications in clinical diagnosis and biomedical research.
研究领域:
生物化学
·
纳米医学
·
临床诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Jing Liu,Lingfeng Wang,Ronghua Shen,Jie Zhao,Ying Qian
In this study, a new cyanine-based photosensitizer Cy-N-Rh was developed for photodynamic therapy. Based on fluorescence resonance energy transfer (FRET) mechanism, utilizing the absorption of the donor rhodamine (Rh), the acceptor heptamethine cyanine unit (Cy) was indirectly excited to produce singlet oxygen ( 1 O 2 ). The efficiency of energy transfer from the donor Rh to the acceptor Cy was 78.5%. Meanwhile, the singlet oxygen yield of Cy-N-Rh (Φ Δ = 12.00%) was much higher than that of the acceptor Cy (Φ Δ = 4.35%) without FRET. Moreover, the dual cation gave Cy-N-Rh with excellent mitochondria-targeting ability with Pearson’s correlation coefficients of 0.90 and 0.91, respectively. In the MTT test, Cy-N-Rh had low dark cytotoxicity with cell survival rate above 90% and high photo cytotoxicity with cell survival rate below 40%. The cell apoptosis assay also demonstrated the role of the photosensitizer Cy-N-R visually. More importantly, Cy-N-Rh fulfilled two-photon excitation fluorescence imaging under the 800 nm femtosecond laser. All results indicate that this design strategy provides a new method for the development of higher-level cyanine photosensitizers.
研究领域:
生物医学工程
·
药学
·
细胞生物学
·
光化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2021
·Vol. 26
·期 11
·3180
·
IF 4.412
Yina Cai,Binxue Ren,Chifang Peng,Cunzheng Zhang,Xinlin Wei
New nanocomposites, Fe3O4@Au–FITC, were prepared and explored to develop a fluorescent detection of Pb2+. The Fe3O4@AuNPs–FITC nanocomposites could be etched by Pb2+in the presence of Na2S2O3, leading to fluorescence recovery of FITC quenched by Fe3O4@Au nanocomposites. With the increase of Pb2+concentration, the fluorescence recovery of Fe3O4@AuNPs–FITC increased gradually. Under optimized conditions, a detection limit of 5.2 nmol/L of Pb2+with a linear range of 0.02–2.0 µmol/L were obtained. The assay demonstrated negligible response to common metal ions. Recoveries of 98.2–106.4% were obtained when this fluorescent method was applied in detecting Pb2+spiked in a lake-water sample. The above results demonstrated the high potential of ion-induced nanomaterial etching in developing robust fluorescent assays.Keywords:gold nanoparticles;magnetic nanoparticles;FRET;nanomaterial etching;lead ions
研究领域:
分析化学
·
环境科学
✓ 已验证
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2014
·
IF 6.451
Zhao Sheng Qian,Xiao Yue Shan,Lu Jing Chai,Juan Juan Ma,Jian Rong Chen,Hui Feng
An ultrasensitive nanosensor based on fluorescence resonance energy transfer (FRET) between biocompatible graphene quantum dots and carbon nanotubes for DNA detection was reported. We take advantage of good biocompatibility and strong fluorescence of graphene quantum dots, base pairing specificity of DNA and unique fluorescence resonance energy transfer between graphene quantum dots and carbon nanotubes to achieve the analysis of low concentrations of DNA. Graphene quantum dots with high quantum yield up to 0.20 were prepared and served as the fluorophore of DNA probe . FRET process between graphene quantum dots-labeled probe and oxidized carbon nanotubes is easily achieved due to their efficient self-assembly through specific π – π interaction. This nanosensor can distinguish complementary and mismatched nucleic acid sequences with high sensitivity and good reproducibility. The detection method based on this nanosensor possesses a broad linear span of up to 133.0 nM and ultralow detection limit of 0.4 nM. The constructed nanosensor is expected to be highly biocompatible because of all its components with excellent biocompatibility.
研究领域:
纳米技术
·
生物传感器
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF LUMINESCENCE
·
2025
·
IF 3.6
Shuo Zhang,Yudan Xie,Xiaoke Hu,Xiaolong Zheng,Ping Huang,Xiaoyue Wang,Hao Zhang,Wei Zheng,Xueyuan Chen
Biotin, as a key nutrition fortifier in infant formula, is of vital significance for guaranteeing the healthy growth of infants and young children. The accurate detection of biotin has therefore become a crucial priority for quality assurance in infant formula. However, the current bioassay techniques encounter a series of limitations such as cumbersome procedures and low sensitivity. Herein, we develop a sensitive time-resolved fluorescence resonance energy transfer (TR-FRET) nanoprobe by employing LiLuF 4 : Ce/Tb nanoparticles (NPs) as energy donors for biotin detection. By taking advantage of the distinctive millisecond-range photoluminescence of the NPs, the short-lived background autofluorescence can be completely eliminated, which enables the sensitive detection of biotin in infant formula within a range of 0−6104 pM and a detection limit down to 0.84 pM (0.21 pg mL −1 ). This value indicates 2−4 orders of magnitude improvement over conventional bioassay methods. The proposed TR-FRET biosensor is independent of expensive instruments and can be fully compatible with commercial microplate readers. Specifically, the homogeneous assay design enables rapid, user-friendly analysis, completing the entire detection process within 20 minutes. These findings offer new opportunities towards rapid and sensitive detection of biotin in infant nutrition products, thereby demonstrating the great potential of lanthanide nanoprobes in food safety monitoring applications.
研究领域:
分析化学
·
食品安全
·
生物医学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）