POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2025
·
IF 6.8
Hua Huang,Jiaying Yu
The cuticle layer covering the surface of the fruit acts as a crucial barrier to regulate water loss and preserve the texture. This study comprehensively investigated the morphological characteristics, cutin matrix depolymerization, and wax content and their effects on transpiration in Chinese olive fruit. Microscopic examination revealed that the surface of the fruit had fissured wax layers, sparse lenticels, and a delicate cuticle covering the epidermal cells. We developed a simple and efficient powder extraction method to decompose cutin polymers, especially from partially depolymerized cutin matrices and epidermal peels, from which cuticular membranes cannot be isolated. The analysis revealed a cutin monomer content of approximately 4.85 g m −2 , predominantly comprising C16–9/10,16-dihydroxy fatty acids (51.4 %) and phenols (19.5 %). The fruit waxes (8.11 g m −2 ) were predominantly composed of pentacyclic triterpenoids, constituting approximately 88 %, with a small amount of very-long-chain aliphatic compounds. The water loss rate from Chinese olive fruit was 4.75 × 10 −3 g m −2 s −1 , resulting in a permeability of 19.6 × 10 −5 m s −1 , which is higher than that reported for many other fruit. The reduced efficiency of the transpiration barrier may largely be attributed to the chemical composition and spatial arrangement of the cuticle. The findings of this study provide a basis for mitigating the dehydration process and developing appropriate preservation strategies for Chinese olive fruit.
研究领域:
植物生理学
·
园艺学
✓ 已验证
DOI
高影响因子
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
·
2025
·
IF 3.7
Shiyan Liu,Xuefeng Zhang,Xin Gong,Jinxin Yu,Tao Lin,Qian Xiang,Xinnian Zeng,Jiali Liu
Dopamine (DA) is a critical molecule within the insect nervous system, known to regulate a myriad of physiological functions and instigate behavioral shifts in insects. It exerts its effects by interacting with specific dopamine receptors (DARs). In this study, three DARs cDNAs from Bactrocera dorsalis (Hendel) (Diptera: Tephritidae) ( BdDOP1 , BdDOP2 and BdDOP3 ) were cloned using molecular biology techniques. These receptors exhibited high sequence identity with their orthologous DARs, and phylogenetic analyses also clustered these receptors within their respective receptor subtype. Additionally, the high expression levels of these DARs in the head suggest their prominent role in the central nervous system of B. dorsalis . To investigate the pharmacological properties of these receptors, expression vectors for BdDOP1 , BdDOP2 and BdDOP3 were constructed and expressed in HEK-293T cells. Our results demonstrated that DA and synthetic agonists activated these receptors in a dose-dependent manner, and DA activation can be competitively inhibited by various antagonists, exhibiting distinct potencies for each dopamine receptor type. Among the tested antagonists, SCH-23390, methiothepin, and metoclopramide were identified as the most potent inhibitors of BdDOP1 , BdDOP2 and BdDOP3 , respectively. This study provides valuable insights into the molecular and pharmacological characteristics of DARs in B. dorsalis , offering a theoretical foundation for the development of novel behavioral modulators targeting these receptors. The findings also serve as a reference for the functional analyses of DARs in other insect species.
研究领域:
昆虫学
·
神经生物学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Qunna Xu,Yao Pu,Jiaqi Shi,Xiaoyu Xu,Jianzhong Ma
Natural food preservatives composed of environmentally-friendly, non-toxic, and biodegradable components have gradually become excellent alternatives to traditional food preservatives. This study developed a fully biomass-based, non-toxic, and eco-friendly food preservation material by encapsulating oregano essential oil (OEO) in self-assembled casein (CA)/chitosan (CS) microcapsules crosslinked with tannic acid (TA) via multiple sites. OEO was successfully encapsulated with an efficiency of 92.5 %. Structural analysis revealed that TA crosslinking significantly altered the secondary structure of casein (increasing α-helix content) and enhanced thermal stability. In food-simulating media, the microcapsules exhibited sustained OEO release across four typical environments, confirming their potential for extensive food preservation applications. The microencapsulated emulsion exhibited prolonged antioxidant and antibacterial activities compared with free OEO. Fruit preservation tests verified that the emulsion effectively extended the shelf life of perishable fruits under ambient conditions. Notably, the use of food-grade materials and a TA-mediated multipoint cross-linking strategy not only enhanced microcapsule stability but also offers an innovative approach for developing safe and sustainable food preservatives.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Chemistry-X
·
2025
·
IF 8.2
Qianwei Qu,Huiwen Wang,Xiaohan Chang,Yue Zhang,Kang An,Yanru Wei,Jingyou Hao,Xueying Chen,Na Dong,Zhiyun Zhang,Yanhua Li
Phyllanthus emblica L . ( P. emblica L.) is a medicine and food homologous plant, whose fruit is acknowledged by the International Health Organization for its high nutritional value. However, eco-friendly and highly efficient methods for extracting the active components from P. emblica L. fruits are currently lacking. In this study, we explored the potential of an eco-friendly enzyme-assisted extraction method for enhancing the extraction of phenolic compounds from P. emblica L., then optimized the extraction process using response surface methodology. After optimization, the contents of gallic acid and total polyphenols reached 33.98 ± 0.15 mg/g and 147.92 ± 1.21 mg GAE/g, respectively. The optimized P. emblica L. extract showed promising anti-inflammatory, antioxidant, and hypoglycemic properties. These findings suggest a viable alternative for obtaining multifunctional extracts from P. emblica L., which have important applications in the functional food and pharmaceutical sectors.
研究领域:
食品科学
·
药学
·
植物生物化学
·
生物技术
·
营养学
·
天然产物研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMISTRY & BIODIVERSITY
·
2025
·
IF 2.5
Jiafu Lin,Jihui Lin,XiaoYu Tao,Dan Zhang,Siwei Chen,Xiujuan Fu,Yu Luo,Jiaqi Zheng,Linyu Liu,Hong Niu
Pectin derived from the fruit of Clausena lansium (Lour.) Skeels, commonly known as Wampee, possesses diverse bioactivities and holds significant potential for applications in food products. To broaden Wampee's application range, deep eutectic solvent (DES) was optimized for pectin extraction using response surface methodology. The fundamental and functional properties of DES-extracted pectin (D-CP) were compared to water-extracted pectin (W-CP). Extracted under the optimal extraction conditions (oxalic acid:choline chloride ratio was 1:1, reagent content was 10.2% (w/v), extraction time was 154 min, temperature was 82.5°C, and liquid: solid was 10.0 mL/g), D-CP was identified as Rhamnogalacturonan-dominated pectin. D-CP's molecular weight (129 kDa) and degree of esterification (61.05%) were lower than those of W-CP. However, D-CP exhibited a superior yield (18.09%) and enhanced functional properties, including higher purity, improved emulsifying stability and activity, better foaming capacity, and stronger foam stability. Additionally, D-CP demonstrated excellent solubility alongside notable antioxidant activities. Overall, these advanced properties highlight D-CP's promising potential for applications in the food and pharmaceutical industries, with DES proving to be an effective solvent for efficient extraction of bioactive pectin from Wampee. Graphical
研究领域:
食品科学
·
天然产物化学
·
生物高分子
·
植物资源利用
·
功能食品
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PLANT GROWTH REGULATION
·
2025
·
IF 4.4
Jin Peng,Xu Huiting,Guo Yongjun,Liu Silin,Bai Cuihua,Cao Laixin,Yao Lixian
Feizixiao litchi ( Litchi chinensis Sonn.), the most widely cultivated commercial variety in China, holds significant economic and social value. However, uneven coloration on the fruit surface markedly diminishes its market appeal. This study investigated the effect of foliar application of ZnO (100 mg/L) and TiO 2 (50 mg/L) nanoparticles (NPs) on the color and quality of Feizixiao during fruit expansion, with water sprayed fruits serving as the control. At harvest, fruits treated with ZnO NPs (63.4 ± 9.5%) and TiO 2 NPs (70.4 ± 8.8%) exhibited significantly enhanced skin coloration rates compared to the control (53.3 ± 9.2%). RNA sequencing of pericarp tissues revealed that the genes associated with anthocyanin biosynthetic enzymes (DFR and UFGT) and transcription factors ( MYB, bHLH, WD40 , and NAC ) were upregulated by both ZnO and TiO 2 NPs sprays , leading to enhanced anthocyanidin synthesis and concomitant fruit pigmentation. Meanwhile, ZnO NPs treatment upregulated the expression of genes encoding Mg and Zn transporters, leading to elevated pericarp Mg and Zn. However, TiO 2 NPs spray reduced S assimilation in pericarp by downregulating the expression SULTR3;4 gene, which was negatively associated with enhanced photosynthesis as well as photorespiration. The alteration of pericarp Mg, Zn, and S was conducive to anthocyanidin synthesis and accumulation. Therefore, ZnO NPs treatment promoted fruit pigmentation by increasing pericarp Mg, Zn, Cy3R, and phenolic copigments accumulation (EC, rutin), while TiO 2 NPs spray enhanced coloration by reducing pericarp S and increasing Cy3R and copigments (PC B2, Qu3G, ferulic acid, and Ka3G). Overall, this study offers a viable strategy for improving fruit coloration in litchi.
研究领域:
植物生理学
·
农业生物技术
·
分子生物学
·
果树栽培学
·
纳米材料应用
✓ 已验证
产品关联
DOI
高影响因子
POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2024
·
IF 6.4
Qiuxiao Yang,Dingding Shi,Yanling Ren,Chao Yang,Hongxia Qu,Yueming Jiang,Taotao Li
Pericarp browning is a vital factor limiting postharvest life of litchi fruit. The influence of vitexin on pericarp browning as well as the related mechanisms in litchi fruit were investigated. Results demonstrated that 1 mM vitexin ameliorated pericarp browning when litchi fruit were stored at 25 °C. Meanwhile, vitexin blocked reactive oxygen species (ROS) production but increased the glutathione peroxidase activity, thereby playing a protective role in oxidative injury. Additionally, vitexin attenuated ATP content decline in relation to regulation of LcSnRK1 as well as LcbZIP1/3 expressions. Furthermore, vitexin regulated expression levels of autophagy-related genes. Finally, LcbZIP3 activated the expressions of LcATG2 and Lcbeclin-1-like protein based on dual-luciferase report (DLR) assay. This is the first report that confers vitexin-meditated protective role in delaying litchi pericarp browning that could be linked to its effects on autophagy particularly regulated via LcSnRK1a–LcbZIP1/3 signaling pathway. Vitexin treatment might be an effective approach to control postharvest senescence of fruit during storage.
研究领域:
植物科学
·
采后生理学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Huihui Wang,Aying Wen,Yuhang Du,Shaofeng Yuan,Hang Yu,Weirong Yao
Chemical detergents are extensively used to remove pesticide residues and foodborne pathogens on fresh fruits, but their residues are toxic to humans. To address this issue, a dual-functional starch copolymer, specifically dialdehyde starch-grafted ε-polylysine copolymer (DIA-ε-PL starch), was synthesized utilizing native starch with surfactant properties and the natural antimicrobial cationic peptide ε-polylysine (ε-PL). The effectiveness of DIA-ε-PL starch as a fruit detergent was subsequently evaluated. Raman imaging and HPLC results indicated that the removal efficiency of chlorothalonil (CHT) reached 96.62% under the optimal conditions which involved washing apples with a 1% corn-DIA-0.16-ε-PL solution at 30°C for 15 min. Compared to traditional washing reagents, DIA-ε-PL starch not only exhibited higher CHT removal efficiency without migrating into apple tissues but also demonstrated superior antibacterial effect against S. aureus (94.64%) and E. coli (98.78%). The DIA-ε-PL starch exhibits significant potential as a fruit-specific detergent due to its non-toxicity, economy, sustainability, and eco-friendliness.
研究领域:
食品化学
·
高分子科学
·
环境科学
·
农业技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2024
·
IF 4
Kaiyang Zhu,Ji Ma,Shuqi Hao,Ting Zhang,Hongjie Lei,Wenge Zhao,Huaide Xu,Mei Li
Oil body (OB) is composed of phospholipids, proteins and neutral lipids. However, the changes of these components in walnut OB (WOB) during walnut growth have not been studied. In this paper, CLSM, TEM and LC-MS/MS were used to observe and quantify the microstructure, accumulation level and biochemical composition of WOB during the growth of walnut (Wen 185 cultivar). The results showed that WOB accumulated near the cell wall on day 60 and then toward the center of cell until it occupied the entire cell on day 88. Afterwards, the WOB began to grow and fuse. Analysis of the chemical composition of WOB indicated that phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, and phosphatidylserine and oleosins, caleosins, and steroleosins made up the WOB membrane. The core of the WOB consisted of 22 free fatty acids and monoacylglycerol, diglyceride, triglyceride. Phosphatidylcholine, triglyceride and oleosin contents continued to increase. The contents of phosphatidylglycerol, unsaturated fatty acids, caleosins and steroleosins continued to decline. The content of phosphatidylethanolamine, phosphatidylinositol and phosphatidylserine remained basically unchanged. These findings provided a basis for understanding the dynamic pattern of WOB bioaccumulation in walnut kernel at different developmental stages.
研究领域:
植物生理学
·
脂类生物化学
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2025
·
IF 6.8
Yuanyuan Chen,Yigui Zeng,Yi Li,Zimao Ye,Liulin Li,Zhiqin Zhou
Spot disease caused by Alternaria alternata ( A. alternata ) commonly occurs in postharvest pears, often resulting in severe rot and considerable economic losses. Here, citral, a natural compound, was found to exhibit potential inhibitory effects against A. alternata . The compound could markedly suppress mycelia growth, with a minimum inhibitory concentration of 0.156 g/L, and simultaneously reduce pear fruit decay. Further analysis revealed that citral altered the hyphal morphology of A. alternata and damaged the integrity of cellular membranes, leading to the release of nucleic acids and proteins. In addition, citral treatment was found to inhibit antioxidant enzyme activity, induce the accumulation of reactive oxygen species, and elevated the malondialdehyde level. It also resulted in a dose-dependent reduction in total cellular lipids, proteins, and sugars. Transcriptomic analysis revealed 1658 upregulated and 2741 downregulated genes in the citral treatment group. Among them, genes related to cell membrane lipids, such as those involved in lipid metabolism, glycerolipid metabolism, steroid biosynthesis, and sphingolipid biosynthesis, were notably enriched. Citral also disturbed fungal energy metabolism by affecting glycolysis/gluconeogenesis, the TCA cycle, and galactose metabolism pathways, which was also confirmed by metabolomic analysis. The results suggest that citral can effectively inhibit A. alternata growth by damaging membrane integrity, activating oxidative stress, and disturbing energy metabolism. Our findings provide new insights into understanding citral as a natural antifungal agent for controlling postharvest black spot disease in pears.
研究领域:
植物学
·
真菌学
·
植物病理学
·
食品科学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Science-Advanced Materials and Devices
·
2024
·
IF 6.7
Zhikun Zhang,Liwei Bai,Tianyi Liu,Huanshu Zhang,Zhengjie Li,Qingju Liu,Jilong Han
Ethephon (ETH) is a plant growth regulator extensively utilized in agriculture, but its overuse is associated with several health issues. However, detecting ETH sensitively and easily in the field is challenging due to the complexity and time-consuming operation of current approaches. In this study, we synthesized aluminum-based metal-organic frameworks (CAU-1) as fluorescent probes for the detection of ETH in fruit. ETH exposure enhanced and shifted the light green fluorescence of CAU-1 to blue through high absorption of phosphate by electrostatic attraction. We observed a linear correlation between fluorescent intensity and ETH concentration ranging from 2 mg/L to 400 mg/L, with the lowest detection limit at 1 mg/L. This fluorescent analysis exhibited good selectivity towards ETH. To facilitate easy identification of samples in the field, we designed a fluorescent paper-based platform with a logic gate operation, allowing visual distinction of samples containing ETH. We applied this platform to monitor ETH in various fruits, including apples, pears, and tomatoes, through fluorescent spectrum, and visual detection. The platform offered simplicity, speed, ease of use and sensitivity, providing a promising future for ETH detection in agriculture.
研究领域:
农业科学
·
分析化学
·
植物生理学
✓ 已验证
DOI
高影响因子
Food Packaging and Shelf Life
·
2024
·
IF 8.5
Bangfeng Fu,Liang Zhang,Peng Zhao,Weijie Gong,Linpin Luo,Bingzhi Li,Xiaoyue Yue,Jianlong Wang,Yiping Chen
The growing demands for fruit preservation have inspired researchers to concentrate on developing advanced food packaging films with sustained and efficient antimicrobial properties. Herein, a visible light-powered photodynamic antimicrobial packaging system with enhanced reactive oxygen species (ROS) production capability (Bi/BiOI 1-x -CS) was developed by demand-driven engineering of the bandgap structure of the photo-responsive filler (Bi/BiOI 1-x ) and the ingenious selection of chitosan matrix (CS) with bacteria capture and killing capabilities. This film displayed excellent photocatalytic killing performance against Staphylococcus aureus ( S. aureus ) and Escherichia coli ( E. coli ) under the visible light, with the bactericidal efficiency up to 99.36 % and 99.54 %, respectively. The enhancement stemmed from the reasonable optimization of the following two aspects: (i) The iodine defects and lateral modification of pure bismuth jointly optimized the electronic band structure of BiOI and significantly boosted the ROS production level, and (ii) The capturing characteristic of CS on bacteria might shorten the diffusion distance between ROS and bacteria, thus further amplifying the bactericidal effect of ROS. As a result, the as-prepared Bi/BiOI 0.20 1-x -CS film (0.20 shows the Bi/BiOI 1-x amount in film solution) exhibited enhanced preservation ability, significantly extending the shelf life of kumquats from 18 to 27 days. Meanwhile, the introduction of Bi/BiOI 1-x successfully promoted the mechanical properties, ultraviolet-barrier performance, and hydrophobicity of CS film. The enhancement of comprehensive performance underscores the potential application of Bi/BiOI 1-x -CS film in the field of fruit preservation.
研究领域:
材料科学
·
食品科学
·
纳米技术
·
生物技术
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）