Journal of Materials Chemistry C
·
2024
·Vol. 12
·期 27
·10135-10144
·
IF 5.7
Bairui Qi,Wenfang Wu,Ziyi Wang,Gengyan Liu,Zhou Li,Zhu Xiao
Chemodynamic therapy is an emerging therapeutic modality that utilizes the Fenton reaction for the production of reactive oxygen species to selectively target and kill cancer cells. In recent years, there has been a notable increase in the demand for developing nanomedicines with enhanced Fenton catalytic efficiency, self-generating H2O2 capability, and superior biocompatibility. This study focuses on the synthesis of a new nanomedicine, PLGA@carbon quantum dots-copper peroxide nanoparticles (PLGA@CQDs–CuPox NPs). The CQD nanodots exhibited the highest fluorescence intensity at an excitation wavelength of 400 nm. CuPox nanodots exhibited superior Fenton catalytic performance at a pH value of 5.5. In vitro experiments demonstrated that PLGA@CQDs–CuPox NPs achieved a killing rate of over 85% for tumor cells while showing a lower killing rate of 10% for normal cells. Upon entering tumor cells, PLGA@CQDs–CuPox NPs underwent degradation, releasing CQDs and CuPox nanodots. CQD nanodots emitted fluorescence, whereas CuPox nanodots decomposed to initiate a Fenton-like reaction. Through the enhanced permeability and retention effect, these nanoparticles penetrated deeper into the tumor cells, enabling deep-seated chemodynamic therapy. Uptake experiments further demonstrated that PLGA@CQDs–CuPox NPs escaped from lysosomes and entered the cell nucleus, utilizing the fluorescence changes of CQD nanodots to determine the optimal treatment time. The design of PLGA@CQDs–CuPox NPs offers a novel approach for precise cancer diagnosis and effective treatment through fluorescence imaging-guided chemodynamic therapy based on tumor microenvironment responsiveness.
研究领域:
纳米技术
·
生物医学工程
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癌症研究
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2018
·
IF 3.909
Qiang Chu,Meng Chen,Dongxiao Song,Xixi Li,Yunyun Yang,Zihuan Zheng,Yonglu Li,Yangyang Liu,Lushuang Yu,Zheng Hua,Xiaodong Zheng
Oxidative stress is a critical cause for the progression of neurodegenerative diseases, indicating that screening natural antioxidant and pharmacological targeting of mechanism need more attention. Natural plant polysaccharides are the primary group of natural antioxidants showing mighty antioxidant activity. Therefore, a polysaccharide (AFP-2) from flowers of Apios americana Medik was isolated, purified and characterized. Then, a H 2 O 2 -induced PC12 cells oxidative stress model was established to investigate the neuroprotective role of AFP-2 and to clarify the potential mechanism of action against H 2 O 2 -induced neurotoxicity. It was found that AFP-2 reduced ROS production and mitochondrial damage caused by hydrogen peroxide in PC12 cells. In addition, data from transmission electron microscope and western blot displayed that AFP-2 significantly activated autophagy in PC12 cells via Akt-mTOR pathway. These results together qualify AFP-2 as an interesting natural polysaccharide worth further investigation as a neuroprotective agent.
研究领域:
神经科学
·
药理学
·
分子生物学
·
天然产物化学
✓ 已验证
DOI
PubMed
高影响因子
CHEMISTRY-A EUROPEAN JOURNAL
·
2024
·
IF 3.9
Xia Wen,Jiancong Ni,Shunmu Zeng,Zhiping Song,Weiwei Qiu
The sensitive and reliable nanozyme-based sensor enables the detection of low concentrations of H2O2 in biological microenvironments, it has potential applications as an in-situ monitoring platform for cellular H2O2 release. The uniformly dispersed bimetallic sulfide (Zn2SnS4) nanoflowers were synthesized via a one-pot hydrothermal method and the two kinds of metal ions can serve as morphology and structure directing agents for each other in the synthetic process. The nanoparticles were utilized as nanozyme materials to fabricate a novel electrochemical sensor, and it exhibits a distinct electrochemical response towards H2O2 with excellent stability and detection capability (with a minimum detection limit of 1.79 nM (S/N = 3)), the excellent characteristics facilitate the precise detection of low concentrations of H2O2 in biological microenvironments. Use the macrophages differentiated from leukemia THP-1 cells as a representative sensing model, the sensor was successfully utilized for real-time monitoring of the release of H2O2 induced by living cells, which has significant potential applications in clinical diagnosis and cancer treatment.
研究领域:
生物传感
·
材料科学
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医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Xue-Liang Liu,Mengdi Yan,Zhi-guo Chen,Bingxin Zhang,Ningcong Yao,Shan Zhao,Xiaoxia Zhao,Tao Zhang,Guangfan Hai
In this paper, we reported a coumarin-based fluorescent probe for selective detection of H 2 O 2 /SO 2 derivatives via ICT process. To the best of our knowledge, it was few reported with the same probe to enable visual detection of H 2 O 2 /SO 2 derivatives in vivo and in vitro. H 2 O 2 and SO 3 2− were selectively sensed over other analytes, and the probe displayed 20-fold and 220-fold relative fluorescence intensity respectively, as well as the good linear relationship and the excellent detection limits of 2.7 * 10 3 nM and 19.3 nM. Furthermore, the probe was successfully used for fluorescence imaging of the HeLa cells and the mice to monitor exogenous and endogenous H 2 O 2 and SO 3 2− , suggesting its potential biomedical application for investigation and detection the intermediate indicator of oxidative stress in vitro and in vivo.
研究领域:
分析化学
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生物医学工程
·
细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2021
·Vol. 45
·期 41
·19565-19571
·
IF 3.591
Peipei Yang,Qingfan Ren,Yuying Chen,Sixue Ouyang,Zhipeng Huang,Peng Zhao,Jia Tao
Alloy nanoparticle-based nanozymes exhibit unique catalytic properties resulting from their tailored atomic coordination numbers and geometric parameters. The development of ultrafine alloy nanozymes is effective for improving their catalytic activities and promoting multifunction. However, it remains a challenge because of the aggregation of alloy nanoparticles resulting from the high surface free energy. This paper reports a strategy for stabilizing ultrafine and uniform CuPd alloy nanoparticles (2.18 ± 0.53 nm) in MIL-101 as a biosensor platform. The steady-state kinetic studies show that CuPd@MIL-101 can serve as a robust peroxidase-like nanozyme with a lower Michaelis constant (Km) than horseradish peroxidase (HRP). H2O2 and glucose were chosen as model targets to develop a sensitive label-free colorimetric method using CuPd@MIL-101. The linear detection of H2O2 in the range of 0.25–700 μM was achieved with a detection limit of 0.043 μM and that of glucose in the linear range of 0.5–400 μM with a detection limit of 0.16 μM. The catalytic activity of CuPd@MIL-101 can remain stable in the dulbecco's modified eagle's medium (DMEM) high-glucose medium and phosphate buffer solution (PBS) for at least five days. This study provides a meaningful reference for developing ultrafine nanozymes without affecting their stability.
研究领域:
生物传感
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纳米医学
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催化化学
✓ 已验证
DOI
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Mengli Xu,Yuan Shen,Linyu Wang,Coucong Gong,Shouhui Chen
In this work, a novel H 2 O 2 biosensor was developed based on the composites of microperoxidase-11 (MP-11) encapsulated in graphene oxide (GO)-PCN-333 (Al) (PCN stands for porous coordination network, one of the metal-organic frameworks). The PCN-333(Al) firmly and uniformly grew on the surface of GO sheet with large specific surface and excellent electrical conductivity, which provided a lots of pores for the encapsulation of MP-11 in the composites and selectively accumulated analytes into its mesopores to improve the selectivity of enzymatic reaction. The MP-11 molecules might freely move through mesopores of PCN-333 (Al) to maintain bioactivity, which remarkably enhanced the mass transfer rate and quickened the electron transfer for electrochemical biosensors. Scanning electron microscopy was used to characterize the GO and PCN-333(Al)-GO composites. Cyclic voltammetry and differential pulse voltammetry were used to characterize the electrochemical behaviors and performance of the H 2 O 2 biosensor. The target electrode showed excellent performance with a wide linear range from 10 μ M to 800 μ M, a low detection limit of 3 μ M, good stability and high selectivity, which was superior to some other H 2 O 2 biosensors.
研究领域:
生物化学
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材料科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Fuqiang Shi,Jie Chen,Lesan Yan,Jing Tu
Chemodynamic therapy (CDT), an innovative approach for treating bacterial infections, has garnered significant attention due to its ability to generate hydroxyl radicals (•OH) via Fenton/Fenton-like reactions. However, the effectiveness of CDT is considerably hindered by the limited availability of endogenous hydrogen peroxide (H2O2) and the overexpression of glutathione (GSH) within the infection microenvironment. To address these limitations, a multifunctional nanoplatform with self-supplying H2O2, GSH-depletion properties, and photothermal properties was developed through a straightforward and mild strategy. This platform employs calcium peroxide (CaO2) as the core, coated with silica (SiO2) to enhance stability and further modified with a Cu(II)-doped polydopamine (PDA) layer, forming a core–shell structured CaO2@SiO2@PDA-Cu (CSPC). The Cu(II) released by CSPC, combined with the H2O2 produced from CaO2 degradation, participates in a Fenton-like reaction to generate toxic •OH radicals. Additionally, Cu(II)-mediated redox reactions deplete overexpressed GSH, thereby enhancing CDT efficacy. Upon coordination with Cu(II), the photothermal properties of PDA are significantly enhanced, achieving a photothermal conversion efficiency of up to 43%. The hyperthermia induced by photothermal therapy (PTT) further increases •OH production, augmenting CDT. The CSPC nanomaterials demonstrated outstanding synergistic photothermal bactericidal activity against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) at 60 μg/mL, achieving complete eradication. Moreover, CSPC eliminated 65.90 ± 3.46% of the S. aureus biofilm under near-infrared (NIR) irradiation. In vivo experiments demonstrated that CSPC treatment effectively eradicated bacteria, with a bacterial survival rate of 6.56 ± 3.28%, and accelerated wound healing, reducing the relative wound size to 7.0 ± 2.6%. Therefore, this study successfully developed versatile nanomaterials that significantly enhance the PTT/CDT dual-mode antibacterial performance.
研究领域:
生物医学工程
·
纳米医学
·
抗菌治疗
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2019
·
IF 2.931
Jiaqi Xian,Yuhui Weng,Hantao Guo,Yan Li,Bixia Yao,Wen Weng
Iron-doped carbon nitride nanoparticles (Fe-CNNPs) were prepared from citric acid , urea and ferric chloride through a convenient one-pot solvothermal method . Oleic acid was used as the reaction medium. The morphology and chemical composition of the obtained Fe-CNNPs were characterized by multiple methods including transmission electron microscopy (TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectroscopy (FT-IR). It is interesting to find that the Fe-CNNPs showed higher catalytic activity than horseradish peroxidase (HRP), and tetramethylbenzidine (TMB) can be catalytically oxidized in the presence of H 2 O 2 to produce a color change in aqueous solution. As H 2 O 2 can be generated in the oxidation process of glucose catalyzed by glucose oxidase (GOD), a novel sensitive method for the detection of glucose with a limit of detection (LOD) of 0.29 μM has been developed combined with the catalytic properties of GOD and Fe-CNNPs. The Fe-CNNPs with peroxidase mimetics activity may have potential applications in biotechnology field.
研究领域:
生物化学
·
纳米材料科学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
Advanced Science
·
2019
·Vol. 6
·期 20
·1901461
·
IF 15.804
Xiaojuan Huang,Guoying Deng,Yong Han,Guizhu Yang,Rujia Zou,Zhiyuan Zhang,Shuyang Sun,Junqing Hu
Stimuli-responsive nanomedicines have become a recent research focus as a candidate for cancer treatment because of their effectiveness, sensibility, and minimal invasiveness. In this work, a novel nanosystem is developed based on Cu 2− x S@MnS core–shell nanoparticles (CSNPs) in which the Cu 2− x S core serves as a photosensitizer to generate hyperthermia and reactive oxygen species (ROS), and the MnS shell is used in H 2 O 2 -responsive O 2 production. Cu 2 −x S@MnS CSNPs with an independent core and shell ratio are synthesized by a controllable hot-injection method, resulting in an optimal photothermal (PT) effect with a PT conversion efficiency of up to 47.9%. An enhanced photodynamic (PD) effect also occurs in an H 2 O 2 environment. More significantly, in vivo experiments demonstrate that Cu 2 −x S@MnS CSNPs can mediate tumor shrinkage in both HeLa tumor cell line-derived xenograft (CDX) and head and neck squamous cell carcinoma (HNSCC) patient-derived xenograft (PDX) models, with the capability of being used as a T1-enhanced magnetic resonance (MR) contrast agent. These results suggest the great potential of as-prepared Cu 2 −x S@MnS CSNPs as photo/H 2 O 2 -responsive therapeutic-agents against tumors, even in a complicated and heterogeneous environment, thus promoting the clinical translation of nanomedicine.
研究领域:
生物医学工程
·
纳米医学
·
癌症治疗
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2019
·Vol. 11
·期 8
·1111-1124
·
IF 2.378
Xiaoheng Qi,Huiming Tian,Xueming Dang,Yaofang Fan,Yaobin Zhang,Huimin Zhao
Metal–organic frameworks (MOFs) with a metal coordination structure mimicking that of enzymes have been widely applied in the sensing field for bioassays, and most of the reports focus on single-metal MOFs. However, bimetallic MOFs have fascinating catalytic capabilities due to their bimetallic coordination. Herein, a two-dimensional sheet-like bimetallic Co/Mn-based MOF (Co/Mn-MOF) with peroxidase-like activity was synthesized through a one-step hydrothermal method to accelerate the decomposition of H2O2 with the generation of ˙OH, which further oxidized the classic chromogenic substrate 3,3′,5,5′-tetramethylbenzidine (TMB) into oxTMB. The Michaelis–Menten constants (Km) and maximum initial velocities (Vmax) of the catalyst were found to be 0.27 mM and 1.64 × 10−7 M s−1 for TMB and 0.24 mM and 5.80 × 10−7 M s−1 for H2O2, respectively. Accordingly, we successfully utilized Co/Mn-MOF-1-1-150 to establish a colorimetric detection platform for the detection of H2O2. A linear absorbance response ranging from 1 to 100 μM with the limit of detection of 0.85 μM (S/N = 3) was obtained under the optimized conditions. The LOD of our catalyst is comparable to that of other MOF-based enzyme mimics for colorimetric detection; this can be ascribed to the synergistic catalytic effect between its two metals, its two-dimensional structure with a small diffusion barrier for the substrate molecules, and large exposed surface area. More importantly, using the proposed sensing method, the determination of H2O2 released by living cells and in spiked water samples was achieved. This revealed that the as-prepared bimetallic Co/Mn-MOF could be a promising candidate as a catalytic material due to its peroxidase activity.
研究领域:
材料科学
·
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION
·
2016
·Vol. 34
·期 3
·1600233
·
IF 4.367
Yanzhou Jiang,Yan Gu,Guangdi Nie,Maoqiang Chi,Zezhou Yang,Ce Wang,Yen Wei,Xiaofeng Lu
In this paper, a novel strategy for the fabrication of reduced graphene oxide (rGO)/Cu 8 S 5 /polypyrrole (PPy) composite nanosheets with Cu 8 S 5 nanoparticles and PPy layer anchored on the surface of rGO as peroxidase-like nanocatalyst is reported. During the synthesis, graphene oxide (GO)/CuO composite nanosheets are prepared first and used as templates, then the sulfuration of CuO and polymerization of pyrrole are accompanied with the reduction of GO, resulting in ternary rGO/Cu 8 S 5 /PPy composite nanosheets. The synthesized Cu 8 S 5 nanoparticles with a diameter in the range from tens to hundreds of nanometers are dispersed within PPy decorated rGO nanosheets. The resultant ternary rGO/Cu 8 S 5 /PPy composite nanosheets exhibit a higher peroxidase-like catalytic activity toward the oxidation of 3,3′,5,5′-tetramethylbenzidine in the presence of H 2 O 2 than GO/CuO and rGO/CuS composite nanosheets, revealing a synergistic effect on their activity. The as-prepared rGO/Cu 8 S 5 /PPy platform provides a simple colorimetric approach for the detection of H 2 O 2 and phenol with a high sensitivity. This work offers a new way for the fabrication of rGO-based nanocomposite with superior enzyme-like activity, which displays great potential applications in biocatalysis and environmental monitoring.
研究领域:
材料科学
·
生物催化
·
环境监测
✓ 已验证
产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2018
·Vol. 42
·期 9
·6803-6809
·
IF 3.201
Qimeng Yang,Shiyu Lu,Bolei Shen,Shujuan Bao,Yingshuai Liu
A colorimetric assay for H2O2 that is based on the use of iron hydroxyl phosphate (Fe3(PO4)2(OH)2) microoctahedrons, which are shown to be efficient peroxidase mimics, has been described. The microoctahedrons were prepared via a one-pot hydrothermal method. They catalyze H2O2 reduction and 3,3′,5,5′-tetramethylbenzidine (TMB) oxidation to produce a blue product whose color can be observed visually and measured using spectrophotometry, best at 652 nm. The method was applied for the quantification of H2O2 with a limit of detection (LOD) of 0.17 μM. The scope of the method was further extended to the determination of glucose by using the enzyme glucose oxidase which catalyzes glucose oxidation to produce gluconic acid and H2O2 which was quantified as described above. The response is linear in the 5–100 μM glucose range, and the LOD is 1.2 μM. This work not only reports a new kind of peroxidase mimic, but also develops a simple, robust and sensitive colorimetric sensing platform for H2O2 and glucose detection in biomedicine and food industry.
研究领域:
生物化学
·
医学检测
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）