BIOORGANIC & MEDICINAL CHEMISTRY
·
2023
·
IF 3.5
Yan-Yan Zhu,Zhao-Jie Wang,Meng Zhu,Zhong-Shun Zhou,Bin-Yuan Hu,Mei-Zhen Wei,Yun-Li Zhao,Zhi Dai,Xiao-Dong Luo
It’s urgent to discover new antibiotics along with the increasing emergence and dissemination of multidrug resistant (MDR) bacterial pathogens. In the present investigation, morusin exhibited rapid bactericidal activity against methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus (VRE) by targeting the phospholipid of bacterial inner membrane, increasing membrane rigidity and disrupting bacterial homeostasis together with the membrane permeability, which caused fundamental metabolic disorders. Furthermore, morusin can also accumulate ROS, suppress H 2 S production, and aggravate oxidative damage in bacteria. Importantly, morusin also inhibited the spread of wounds and reduced the bacterial burden in the mouse model of skin infection caused by MRSA. It’s a chance to meet the challenge of existing antibiotic resistance and avoid the development of bacterial resistance, given the multiple targets of morusin.
研究领域:
微生物学
·
药理学
·
传染病学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 8
·1-11
·
IF 5.3
Wang Sailan,Huang Ling,Ma Guangran,Xu Fugang
A hybrid material GO@Ag composed of graphene oxide (GO) decorated with silver nanoparticles (AgNPs) was prepared for sensitive detection of hydrogen sulfide (H 2 S), a food spoilage indicator and a toxic gas. The GO@Ag nanocomposite demonstrated excellent oxidase-like activity and Raman signal enhancement capability, which can effectively catalyze colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to produce oxidized TMB (ox-TMB) with blue color and strong surface-enhanced Raman scattering (SERS) signals. The presence of H 2 S reacts with AgNPs on the GO surface to form Ag 2 S, which inhibits the nanozyme activity and SERS enhancement activity of the GO@Ag, resulting in decreased absorbance value and SERS signal, thereby achieving colorimetric and SERS dual-mode detection of H 2 S. The method exhibits a wide linear range of 10 nM ~ 50 μM (colorimetry) and 5 nM ~ 50 μM (SERS) and low detection limits of 9 nM (colorimetry) and 0.9 nM (SERS) with simple operation, good selectivity, and high reproducibility. Furthermore, the composite material has been successfully applied to the detection of H 2 S in actual fish samples, providing a new technical means for gas sensing in food safety monitoring. Graphical
研究领域:
纳米技术
·
分析化学
·
食品安全
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材料科学
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传感器
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环境监测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Omega
·
2022
·
IF 4.132
Xiudi Zhou,Qiang Chen,Chunjing Guo,Yanguo Su,Huimin Guo,Min Cao,Zhongxin Liu,Dandan Zhang,Ningning Diao,Huaying Fan,Daquan Chen
For the therapy attenuating renal ischemia–reperfusion (IR) injury, a novel drug delivery system was urgently needed, which could precisely deliver drugs to the pathological renal tissue. Here, we have prepared new nanomaterials with a reactive oxygen species (ROS)-responsive hydrogen sulfide (H2S) donor and hyaluronic acid that targets CD44 receptor. The novel material was synthesized and characterized via related experiments. Then, rapamycin was loaded, which inhibited kidney damage. In the in vitro study, we found that the micelles had ROS-responsiveness, biocompatibility, and cell penetration. In addition, the experimental results showed that the intracellular H2S concentration after administration was threefold higher than that of the control group. The western blot assay revealed that they have anti-inflammatory effects via H2S donor blocking the NF-κB signaling pathway. Consequently, the rising CD44 receptor-targeting and ROS-sensitive H2S donor micelles would provide a promising way for renal IR injury. This work provides a strategy for improving ischemia/reperfusion injury for pharmaceuticals.
研究领域:
药学
·
纳米技术
·
肾脏生理学
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药物递送系统
·
炎症生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMATERIALS
·
2025
·
IF 12.9
Liyang Gong,Le Chang,Siyu Chen,Xuan Wei,Huiping Du,Jiamin Cheng,Xiaoxuan Chen,Zhang Yuan,Pan Zhao,Meijuan Geng,Hui Yang,Kaiyong Cai,Liangliang Dai
Hydrogen sulfide (H 2 S) gas therapy is beneficial for accelerating wound healing and alleviating the inflammatory process, but is seriously hindered by insufficient delivery and unsustainable release in vivo . This study presents a multifunctional injectable hydrogel, OC@ε-PL-SATO, composed of oxidized hyaluronic acid and N-acetylcysteine (NAC) as an initiator, carboxymethyl chitosan and S-aroylthiooxime modified ε-Poly-( l -lysine) (ε-PL-SATO). ε-PL-SATO is a NAC-responsive H 2 S donor. OC@ε-PL-SATO hydrogel is designed for the desired wound healing process, with rapid gelation (<30 s) and a sustained H 2 S release. After mixing and gelling, H 2 S could be long-term released from the hydrogel and effectively drives macrophages toward M2 polarization, thereby ameliorating the inflammatory response. Revealed by transcriptome analysis, the underlying mechanism is that OC@ε-PL-SATO hydrogel releasing H 2 S inhibits LPS-mediated inflammatory responses in RAW264.7 cells by interfering with phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) signaling and NF-κB activation. Furthermore, the OC@ε-PL-SATO hydrogel effectively eliminates the bacterial burden and alleviates the accompanying inflammation in a rat model of cutaneous wound infection. Importantly, the sustained generation of H 2 S gas significantly promotes angiogenesis and collagen deposition, ultimately accelerating the wound repair. In conclusion, this study provides a multifunctional injectable hydrogel with rapid gelatinization and continuous H 2 S release for accelerating the infected wound healing.
研究领域:
生物材料
·
药剂学
·
伤口愈合
·
免疫学
·
炎症
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2023
·
IF 19
Fan Zhao,Liying Liang,Heng Wang,Chen Wang,Dan Su,Yao Ying,Wangchang Li,Juan Li,Jingwu Zheng,Liang Qiao,Xiaozhou Mou,Shenglei Che,Jing Yu
Copper overload is a novel way to achieve copper-ion-interference therapy by disrupting copper homeostasis and treating diseases through multiple cell death pathways. However, it is difficult to reach copper overload since excess intracellular copper ions will be pumped out. Herein, copper overload is achieved by both raising cellular uptake and reducing the efflux of copper ions using hydrogen sulfide (H 2 S)-responsive copper hydroxyphosphate nanoparticles (Cu 2 (PO 4 )(OH) NPs). After immersion in an H 2 S-enriched colon cancer microenvironment, Cu 2 (PO 4 )(OH) NPs can transform into copper sulfide NPs with reduced size for higher cell entering, resulting in improved Fenton activity as well as copper ion dissociation. Reactive oxygen species generated by the Fenton reaction not only activate inflammasomes and Caspase-1 proteins, cause the cleavage of gasdermin D to induce pyroptosis, but also affect mitochondrial function and down-regulate copper exporter ATP7A to further reduce the copper excretion. The combination of higher endocytosis and lower exportation leads to maximized copper overload. Together with the efficient copper ions release, mitochondrial tricarboxylic acid cycle can be disrupted and iron-sulfur cluster proteins are downregulated, ultimately triggering cuproptosis. As both pyroptosis and cuproptosis are efficient ways to induce cell death, this study provides a novel way to realize effective tumor-targeted therapy based on H 2 S-activated copper overload with simple Cu 2 (PO 4 )(OH) NPs.
研究领域:
肿瘤学
·
纳米技术
·
细胞生物学
·
治疗学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Qiang Chen,Chunjing Guo,Xiudi Zhou,Yanguo Su,Huimin Guo,Min Cao,Jing Li,Yue Zhang,Weiyi Zhao,Xin Gao,Shuqi Mi,Daquan Chen
Currently, very limited therapeutic approaches are available for the drug treatment of atherosclerosis(AS). H 2 S-donor is becoming a common trend in much life-threatening research. Several studies have documented that H 2 S-lyase is predominantly present in endothelial cells. N-Acetylneuraminic acid (SA), natural carbohydrate, binds specifically to the E -selectin receptor of endothelial cells. Meanwhile, recent studies related to Chondroitin sulfate have excellent target binding ability with CD44 receptor. We conjecture that the N-Acetylneuraminic acid and Chondroitin sulfate modified nanomicelles not only enhances the accumulation of the drug but also cleaves the H 2 S donor in the lesion, thus one stone two birds. Given these findings, we synthesized two kinds of nanoparticles, Carrier I (SCCF) and Carrier II (SCTM), for atherosclerosis to validate our guesses. Initially, S-allyl-L-cysteine and 4-methoxyphenylthiourea were used as H 2 S donors for SCCF and SCTM, respectively. After the introduction of ROS-sensitive groups. Then, micelles with N-Acetylneuraminic acid and Chondroitin sulfate were prepared to load rapamycin(RAP). Further, in atherosclerosis Oil Red O staining (ORO) results confirmed remarkable treatment effect with [email protected] and [email protected] Thus, we conclude that the effect of dual-targeting nanomicelles with ROS-sensitive H 2 S donor based on N-Acetylneuraminic acid and Chondroitin sulfate will have a better role in atherosclerosis.
研究领域:
药学
·
纳米技术
·
心血管疾病
·
药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Jiale Chen,Zhixiang Mu,Dongfan Chen,Chen Huang,Ting Jin,Lin Li,Youyun Zeng,Qiang Zhou,Yanmei Zhang,Hongli Mao,Hui Deng,Xinkun Shen,Hong Yang,Xiaojun Cai
Smart hydrogel dressings capable of simultaneously highly effective antimicrobial, anti-inflammatory, and promoting re-epithelialization and angiogenesis are urgently needed for the management of diabetic wounds. Herein, a H 2 S-releasing multifunctional hydrogel was developed by utilizing the dynamic Schiff base reaction between carboxymethyl chitosan (CMC) and polyhexamethylene guanidine (PHMG)-modified aldehyde F108 (PFC). Diallyl trisulfide (DATS) was encapsulated into the PFC nanoparticles. Apart from possessing the essential properties necessary for an idealized hydrogel dressing, such as excellent injectability, tissue adhesion, self-healing, and stimulus–response degradation, the [email protected] &CMC also utilized the synergistic effect of the PHMG and DATS to provide an efficient antimicrobial effect; the H 2 S was slowly released from the DATS under the action of GSH and exerted excellent anti-inflammatory effects, by inhibiting the expression of p-ERK and p-STAT3 in activated macrophages, and promoting macrophage polarization to the M2 phenotype. Strikingly, following the completion of the efficient antimicrobial and anti-inflammatory effects, the continuously generated H 2 S further significantly accelerated the proliferation, migration, and vascularization of endothelial cells by extending the activation of the p-p38 and p-ERK1/2. Owing to superior performances, [email protected] &CMC significantly promoted the healing of diabetic wounds induced by streptozotocin with good biocompatibility. This study demonstrates that [email protected] &CMC is a versatile hydrogel dressing with great potential for the management of diabetic wounds.
研究领域:
生物医学工程
·
材料科学
·
药剂学
·
伤口愈合
·
糖尿病管理
✓ 已验证
产品关联
DOI
高影响因子
Journal of Materials Chemistry B
·
2023
·Vol. 11
·期 45
·10822-10835
·
IF 7
Mingzhe Liu,Hui Xu,FangFang Zhou,Xiyu Gong,Songwen Tan,Yongju He
The high glutathione (GSH) concentration and insufficient H2O2 content in tumor cells strongly constrict the efficacy of Fenton reaction-based chemodynamic therapy (CDT). Despite numerous efforts, it still remains a formidable challenge for achieving satisfactory efficacy using CDT alone. Herein, an intelligent tetrasulfide bond-bridged mesoporous organosilica-based nanoplatform that integrates GSH-depletion, H2S generation, self-supplied H2O2, co-delivery of doxorubicin (DOX) and Fenton reagent Fe2+ is presented for synergistic triple-enhanced CDT/chemotherapy/H2S therapy. Because the tetrasulfide bond is sensitive to GSH, the nanoplatform can effectively consume GSH, leading to ROS accumulation and H2S generation in the GSH-overexpressed tumor microenvironment. Meanwhile, tetrasulfide bond-induced GSH-depletion triggers the degradation of nanoparticles and the release of DOX and Fe2+. Immediately, Fe2+ catalyzes endogenous H2O2 to highly toxic hydroxyl radicals (˙OH) for CDT, and H2S induces mitochondria injury and causes energy deficiency. Of note, H2S can also decrease the decomposition of H2O2 to augment CDT by downregulating catalase. DOX elicits chemotherapy and promotes H2O2 production to provide a sufficient substrate for enhanced CDT. Importantly, the GSH depletion significantly weakens the scavenging effect on the produced ˙OH, guaranteeing the enhanced and highly efficient CDT. Based on the synergistic effect of triple-augmented CDT, H2S therapy and DOX-mediated chemotherapy, the treatment with this nanoplatform gives rise to a superior antitumor outcome.
研究领域:
纳米技术
·
癌症治疗
·
药学
·
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2021
·Vol. 45
·期 13
·5822-5828
·
IF 3.591
Yingwu Wang,Qiang Lin,Ping Ning,Chi Wang,Heping Zeng,Xin Sun,Xin Song,Kai Li
H2S and PH3, which are ore-thermal furnace gases, are harmful to humans and the environment. Here, the performance of a catalyst, Ce–Cu–Al trimetallic composite catalyst, is further explored by examining the influence of the used acid. The effects of acids on the structural features, surface functional groups, and activity of the catalysts were examined. Test results showed that the catalyst fabricated using 71.7 mmol nitric acid performed the best, and its capacity reached 229.8 mg g−1 (H2S) and 201.9 mg g−1 (PH3). Besides, SEM and TEM showed that the whole structure of catalysts was sharply changed by the species of the acid used, which was caused by the different combinations of components in the catalyst system. Because of the change in the catalyst structure, their physical and chemical properties are also adjusted. Using nitric acid could endow the catalyst with better properties, such as the best specific surface area, higher total pore volume, the strongest acid center, and the best reducing power. Combining the analysis results of FTIR spectroscopy, it was found that the detailed functional groups of NH2− and νON played a role in the reaction.
研究领域:
催化
·
环境化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Kai Wang,Xue Yang,Meng-Ya Guo,Xu-Yang Chen,Ting Li,Ru Yan,Yu-Shun Yang,Hai-Liang Zhu,Zhi-Gang Hu
Hydrogen sulfide (H 2 S) is a signal molecule that is significantly involved in cell protection, vascular regulation, and neuro-transmission. Nevertheless, abnormal levels of H 2 S are intimately relevant to Alzheimer’s disease, liver cirrhosis, diabetes mellitus, and Down syndrome. To improve the H 2 S detection in vitro and in vivo , we developed RDM-H 2 S , a novel red-emission fluorescent probe . RDM-H 2 S has a rapid response (120 s), high sensitivity (LOD = 310 nM), obvious response degree (234 folds), relatively long excitation/emission wavelength (565 nm/620 nm), and steady emission in pH 6–9. In addition, RDM-H 2 S showed high specificity to H 2 S in various species. Due to the low cytotoxicity of RDM-H 2 S , it was beneficial to the application of biological depth imaging . In addition, RDM-H 2 S could measure exogenous and endogenous H 2 S in distinct biological models, such as HeLa cells and zebrafish. Besides, RDM-H 2 S had excellent mitochondrial targeting ability and could be used to monitor H 2 S in living mice. RDM-H 2 S could visualize endogenous H 2 S in different tissues and 4T1-xenograft tumor mice. Additionally, RDM-H 2 S also exhibited immense application potential for the visualization of H 2 S in the process of inflammation. RDM-H 2 S was applied for measuring the level of H 2 S in human plasma samples. Consequently, RDM-H 2 S was determined to be a valuable tool for monitoring H 2 S in pre-clinical diagnosis.
研究领域:
生物化学
·
医学诊断
·
分子生物学
✓ 已验证
DOI
高影响因子
Royal Society Open Science
·
2019
·Vol. 6
·期 5
·
IF 2.515
Pingfeng Fu,Gen Li,Xiaoting Wu,Xiaofeng Lin,Bolan Lei
The decomposition of toxic flotation reagents upon UV185+254 nm irradiation was attractive due to operational simplicity and no dosage of oxidants. In this work, the degradation of typical thiol collectors (potassium ethyl xanthate (PEX), sodium diethyl dithiocarbamate (SDD), O-isopropyl-N-ethyl thionocarbamate (IET) and dianilino dithiophoshoric acid (DDA)) was investigated by UV185+254 nm photolysis. The degradation efficiencies and mineralization extents of collectors were assessed. The formation of CS2 and H2S byproducts was studied, and the mechanisms of collector degradation were proposed under UV185+254 nm irradiation. The PEX, SDD and IET were decomposed with nearly 100% removal upon 75 min of UV185+254 nm irradiation. The decomposition rate constants decreased in the order SDD > PEX > IET ≫ DDA, and the DDA was the refractory collector. After 120 min of UV185+254 nm irradiation, 15−45% of carbon and 25−75% of sulfur of collectors were completely mineralized, and the mineralization extent decreased in the order PEX > SDD > IET > DDA. The percentage of gaseous sulfur (CS2 and H2S) ranged from 0.48 to 4.85% for four collectors, showing the fraction of emitted sulfur byproducts was small. The aqueous CS2 concentration increased in the first 10−20 min, and was decreased to a low level of 0.05–0.1 mg l−1 at 120 min. Two mechanisms, i.e. direct UV254 nm photolysis and indirect oxidation with free radicals, were responsible for collector decomposition in the UV185+254 nm photolysis.
研究领域:
环境科学
·
化学工程
✓ 已验证
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2023
·
IF 6.7
Haoming Sun,Zhaorui Zhang,Xiaojing Tian,Manli Lu,Bo Wang,Pinhua Zhang,Guangliang Cui,Lulu Du,Kaifeng Xue
Developing a gas sensor for the expedient, rapid, and accurate detection of hydrogen sulfide (H 2 S) in human exhaled breath at room temperature would offer an opportunity for home-style self-monitoring of some chronic diseases. In this work, Cu 2 O-carbon nanofiber (CNF) heterojunctions were prepared by a two-dimensional (2D) electrodeposition in-situ assembly method to detect low-concentration H 2 S gas at room temperature. The heterojunction had a distinct interface, which was confirmed by scanning electron microscopy, transmission electron microscopy and voltammetry tests. The detection limit of the sensor was as low as 5 ppb, and the sensors could directly distinguish the exhaled breath of healthy individuals and simulated patients. We attributed the excellent performances to synergistic effects of the modulation of the heterointerface barrier and the sulfide reaction of Cu 2 O. Furthermore, the adsorption behaviors of H 2 S on the surfaces of pure Cu 2 O and the Cu 2 O-CNF heterojunction were evaluated using the density functional theory (DFT) based on first principles. The results further confirmed that the formation of the heterostructure enhances the H 2 S adsorption capacity and electron transfer of the sensitive material. This study is of great significance for the diagnosis and monitoring of diseases with the exhaled H 2 S biomarker.
研究领域:
材料科学
·
医学诊断
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）