International Journal of Nanomedicine
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2023
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IF 8
Tingting Song,Hong Wang,Yue Liu,Rongshan Cai,Dezhi Yang,Yongai Xiong
Background Ziyuglycoside I (ZgI), an active ingredient isolated from traditional Chinese medicine Sanguisorba officinalis L, has been demonstrated to increase the leucocytes and protect hematopoietic stem cells. However, the poor solubility and a short half-life of ZgI limit its bioavailability and efficacy. The D-α-tocopherol polyethylene glycol 1000 succinate (TPGS) has been widely used to increase the solubility, improve the encapsulation rate, and extend the half-life of drugs.Methods Here, we formulated the TPGS-modified long-circulating liposomes loading ZgI with a sustained drug release and enhanced therapy for myelosuppression. ZgI-TPGS-liposomes were manufactured using a thin-film hydration technique, followed by characterizations of physicochemical properties, including the particle size, zeta potential, TEM, SEM, FTIR, XRD, stability, drug loading (DL), encapsulation efficiency (EE). The in vitro and in vivo delivery efficiency were further evaluated by cellular uptake, in vitro drug release and in vivo pharmacokinetics. Finally, therapeutic effect on myelosuppression was investigated.Results The ZgI-TPGS-liposomes had an particle size of 97.89 ± 1.42 nm and ZP of −28.65 ± 0.16 mV. It exhibited DL of 9.06 ± 0.76% and EE of 92.34 ± 3.83%, along with excellent storage stability, cellular uptake and sustained drug release to free ZgI and liposomes without TPGS. Additionally, the TPGS modified liposomes significantly enhanced the therapeutic effect of ZgI on CTX induced myelosuppression, which can be confirmed in the apoptosis inhibition and cell viability promotion of CTX injured HSPC-1 cells. Also, the mice in vivo pharmacodynamics demonstrated that TPGS liposomes promoted ZgI increasing the numbers of leucocytes and neutrophils in myelosuppression mice induced by CTX.Conclusion Our research suggest that TPGS-modified long-circulating liposomes loading ziyuglycoside I has potential application in myelosuppression therapy.
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Analytical Methods
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2023
·Vol. 15
·期 12
·1546-1552
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IF 3.1
Yucui Hou,Xiaoling Lu,Jie Yang,Chunhua Tang,Hanbing Jiang,Tongji Cai,Meilun Chen,Zheng Wei,Peng Yu
Testosterone is a steroid hormone that plays an indispensable role in the normal metabolism of organisms. However, exogenous testosterone, even as low as nmol L−1, will harm the human body due to accumulation. In this study, we developed an unlabeled fluorescent sensor for testosterone based on SYBR Green I. SYBR Green I is a fluorescent dye that can be embedded into the G-quadruplex of the testosterone aptamer T5. The fluorescence quenching effect is utilized to achieve quantitative detection, which occurs by the competition between testosterone and SYBR Green I for the T5 aptamer binding sites. In this work, we optimized the detection conditions to make the fluorescent sensor more sensitive and verify the specificity, linear range, and detection ability in the buffer and real water samples. The sensor's LOD and LOQ values were 0.27 nmol L−1 and 0.91 nmol L−1, respectively, while the detection range was linear from 0.91 nmol L−1 to 2000 nmol L−1. According to the results, the sensor shows high specificity and good performance even in real sample detection such as tap water and river water, providing an alternative method for the quantitative detection of testosterone in the environment, which is more convenient and efficient.
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ORGANIC & BIOMOLECULAR CHEMISTRY
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2018
·Vol. 16
·期 48
·9402-9408
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IF 3.423
Pan Li,Zhe Chen,Yishun Huang,Jing Li,Fan Xiao,Shiyao Zhai,Zhiming Wang,Xuanjun Zhang,Leilei Tian
A new DNA-based fluorescent probe, which is a hybrid molecule of an i-motif forming sequence (IFS) and mono-functionalized tetraphenylethene (TPE), has been synthesized and investigated. A distinct pH-responsive aggregation-induced emission (AIE) effect has been observed from this hybrid molecule, i.e. the fluorescence of TPE will be turned on when the IFS part folds up under acidic conditions. According to the fact that a hybrid molecule with a relatively rigid structure shows a more obvious AIE effect, and whose nano-sized aggregates are formed at a high concentration, we assume that the solubility of the hybrid molecule in water will be reduced due to IFS folding, resulting in aggregation and the resultant AIE effect. Finally, due to its excellent pH responsiveness, this DNA-based probe employing an AIEgen has been applied in monitoring intracellular pH.
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ENZYME AND MICROBIAL TECHNOLOGY
·
2019
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IF 3.553
Yuping Shen,Man Wang,Jinwei Zhou,Yufei Chen,Lili Xu,Mengru Wu,Guohua Xia,James P. Tam,Jiangnan Yu,Xiying Teng,Huan Yang,Xiaobin Jia
Baohuoside I, a rare secondary glycoside from Epimedii Folium, has anti-osteoporosis and other pharmacological effects. In addition, it exhibits improved bioactivities compared with its original glycoside icariin. Conventional methods for the production of baohuoside I, such as acid hydrolysis, exhibit very low efficiency with serious pollution and substantial byproducts. The aim of this study was to develop an eco-efficient biphasic enzymatic hydrolysis system for the green production of rare baohuoside I. β -glucanase was selected to hydrolyze icariin and showed better performance than β -glucosidase. The biphasic system was constructed using HAc-NaAc buffer (pH 4.5) containing β -glucanase/icariin (C icariin = 100 mg/mL; 1:1, w/w ) and propyl acetate (1:2, v/v ), and the hydrolysis was performed at 60℃ for 6 h. The hydrolysis capacity of icariin in the system was largely increased from 0.5 mg/mL to 100 mg/mL. The conversion ratio of icariin was greater than 99% in a 100-fold scale-up pilot test, demonstrating its strong potential for industrial applications. Furthermore, a process flow and production plant were designed to produce 1 ton of baohuoside I annually, and the profit would be approximately 4.5-fold its total cost. Biphasic enzymatic hydrolysis is an eco-efficient technology to produce baohuoside I and represents a promising method in the pharmaceutical industry.
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NEW JOURNAL OF CHEMISTRY
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2019
·Vol. 43
·期 34
·13408-13417
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IF 3.069
Qiong Wei,Hong-Ting Chen,Li Liu,Xin-Xin Zhong,Lei Wang,Fa-Bao Li,Heng-Jiang Cong,Wai-Yeung Wong,Khalid A. Alamry,Hai-Mei Qin
High efficiency copper(I) halide complexes containing rigid diphosphine ligands have attracted much attention. However, rigid bidentate phosphine ligands have been rarely reported so far. Here, an electron-rich heteroaryl ring grafted with two electron donating methyl groups was first included to construct a rigid bidentate phosphine ligand, three four-coordinate dinuclear copper(I) halide complexes, [CuX(dpmt)]2 (dpmt = 3,4-bis(diphenylphosphino)-2,5-dimethylthiophene, X = I (1), Br (2) and Cl (3)), were synthesized, and their molecular structures and photophysical properties were investigated. The structural analysis reveals that two copper(I) centers are bridged by two halogen ligands to form a dinuclear structure with a four-membered Cu2X2 ring. These complexes exhibit intense bluish green to yellow green emission in the solid state at room temperature and have peak emission wavelengths at 490–543 nm with microsecond lifetimes (τave = 3.9–13.6 μs, Φ = 0.26–0.35). The emission of complex 1 mainly originates from MLCT and ligand-centered transitions. Similar emissive behaviors for complexes 2 and 3 are possibly due to ligand-centered transition. The solution-processed devices of complex 3 exhibit stable yellow emission with CIE(x, y) of (0.42, 0.52) for doped and nondoped devices.
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FOOD CHEMISTRY
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2018
·
IF 4.946
Diandian Deng,Hong Yang,Chang Liu,Kang Zhao,Jianguo Li,Anping Deng
An immunochromatographic assay (ICA) based on surface-enhanced Raman scattering (SERS) for ultrasensitive determination of Sudan I in food samples was reported. Gold-silver core-shell bimetallic nanorods (referred to as [email protected] NRs) were synthesized, characterized and used as the substrate for preparation of the ICA. Polyclonal antibody against Sudan I was immobilized on the surface of the [email protected] NRs carrying the Raman reporter 5,5′-dithiobis (2-nitrobenzoic acid). The Raman scattering intensity on the test line was used for quantitation of Sudan I. The assay was completed in 15 min. IC 50 and limit of detection (LOD) were 30 pg mL −1 and 0.2 pg mL −1 , respectively. There was no cross-reactivity (CR) of the assay with Sunset Yellow, Lemon Yellow and Brilliant blue FCF, but only 3.53%-9.74% CR with Sudan II, III and IV. The recoveries of Sudan I from spiked food samples were in the range of 88.9–107.6% with relative standard deviation of 3.7–8.7% (n = 3).
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Biomed Research International
·
2018
·
IF 2.583
Yong He,Wei Liu,Lianxiong Guan,Jielin Chen,Li Duan,Zhaofeng Jia,Jianghong Huang,Wencui Li,Jianquan Liu,Jianyi Xiong,Lijun Liu,Daping Wang
Scaffolds play an important role in tissue engineering and their structure and biocompatibility have great influence on cell behaviors. In this study, poly(l-lactide-co-ε-caprolactone) (PLCL) scaffolds were printed by a 3D printing technology, low-temperature deposition manufacturing (LDM), and then PLCL scaffolds were treated by alkali and coated with collagen type I (COLI). The scaffolds were characterized by scanning electron microscopy (SEM), porosity test, mechanical test, and infrared spectroscopy. The prepared PLCL and PLCL-COLI scaffolds had three-dimensional (3D) porous structure and they not only have macropores but also have micropores in the deposited lines. Although the mechanical property of PLCL-COLI was slightly lower than that of PLCL scaffold, the hydrophilicity of PLCL-COLI was significantly enhanced. Rabbit articular chondrocytes were extracted and were identified as chondrocytes by toluidine blue staining. To study the biocompatibility, the chondrocytes were seeded on scaffolds for 1, 3, 5, 7, and 10 days. MTT assay showed that the proliferation of chondrocytes on PLCL-COLI scaffold was better than that on PLCL scaffold. And the morphology of cells on PLCL-COLI after 1-day culture was much better than that on PLCL. This 3D-printed PLCL scaffold coated with COLI shows a great potential application in tissue engineering.
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XENOBIOTICA
·
2018
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IF 1.857
Yue Li,Yujuan Fan,Huizong Su,Qian Wang,Guo-Fu Li,Yiyang Hu,Jian Jiang,Bo Tan,Furong Qiu
Tanshinone I (TSI) is a lipophilic diterpene in Salvia miltiorrhiza with versatile pharmacological activities. However, metabolic pathway of TSI in human is unknown.In this study, we determined major metabolites of TSI using a preparation of human liver microsomes (HLMs) by HPLC-UV and Q-Trap mass spectrometer. A total of 6 metabolites were detected, which indicated the presence of hydroxylation, reduction as well as glucuronidation.Selective chemical inhibition and purified cytochrome P450 (CYP450) isoform screening experiments revealed that CYP2A6 was primarily responsible for TSI Phase I metabolism. Part of generated hydroxylated TSI was glucuronidated via several glucuronosyltransferase (UGT) isoforms including UGT1A1, UGT1A3, UGT1A7, UGT1A9, as well as extrahepatic expressed isoforms UGT1A8 and UGT1A10. TSI could be reduced to a relatively unstable hydroquinone intermediate by NAD(P)H: quinone oxidoreductase 1 (NQO1), and then immediately conjugated with glucuronic acid by a panel of UGTs, especially UGT1A9, UGT1A1 and UGT1A8. Additionally, NQO1 could also reduce hydroxylated TSI to a hydroquinone intermediate, which was immediately glucuronidated by UGT1A1.The study demonstrated that hydroxylation, reduction as well as glucuronidation were the major pathways for TSI biotransformation, and six metabolites generated by CYPs, NQO1 and UGTs were found in HLMs and S9 subcellular fractions.
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NEW JOURNAL OF CHEMISTRY
·
2018
·Vol. 42
·期 20
·16814-16822
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IF 3.201
Wei Huang,Shucheng Liu,Jinxin Liu,Wenli Zhang,Jianming Pan
A facile strategy to fabricate crown ether (2-methylol-12-crown-4, 2M12C4) immobilized porous polymers (PGMA-CE) was reported toward lithium(I) (Li+) recovery. Macroporous polymer foam (polymeric glycidyl methacrylate, PGMA) with abundant epoxy groups was firstly prepared as a support using a high internal phase emulsion (HIPE) template. And then 2M12C4 was covalently attached onto the surface of PGMA to endow the PGMA-CE with good stability and high selectivity. PGMA-CE was characterized by SEM, FT-IR, XPS, and 13C NMR spectroscopy, and its porous structure and the successful introduction of 2M12C4 were confirmed. In batch mode experiments under optimum conditions, PGMA-CE exhibited fast adsorption kinetics (the equilibrium time was 3.0 h), and the kinetics data were well described using a pseudo-second-order model. A Langmuir-type monolayer adsorption was investigated, and the calculated maximum equilibrium adsorption capacity was 3.15 mg g−1. In addition, PGMA-CE displayed excellent selectivity for Li+ in the presence of multiple interfering ions (Na+, K+, Mg+, and Ca2+), and the selective separation factors (α) were above 4.75. Regeneration analysis showed that the adsorption capacity of PGMA-CE toward Li+ after five cycles was 91.8% of the first cycle.
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DALTON TRANSACTIONS
·
2018
·Vol. 47
·期 29
·9845-9849
·
IF 4.099
Daqin Chen,Xiao Chen,Junni Li,Xinyue Li,Jiasong Zhong
The preparation of CsPbX3 nanoplatelets and the investigation of their optical properties have been rarely reported. Herein, the successful development of all-inorganic CsPbX3 (X = Cl, Br, or I) perovskite quantum dots has been achieved and their unique optical and electrical properties are discussed. Apre-dissolution assisted solvothermal reaction was developed to synthesize ultrathin (∼3 nm) CsPbBr3 nanoplatelets with strong exciton absorption, with a 0.41 eV blue-shifted emission relative to that of CsPbBr3 nanocrystals and a high quantum yield of 28%. Anion exchange allowed the variation of the halogen content of the nanoplatelets and allowed them to retain their shape and size and achieve multi-color emission.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2019
·Vol. 30
·期 16
·14977-14986
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IF 2.195
Tang Hongbo,Li Qiuyue,Zhou Jian
The effect of low I/Ag ratios on the performance of silver thick films containing micro-sized silver particles treated with surface iodination has been investigated. The film with I/Ag ratio of 1:100 exhibited high electrical conductivity and densification, while that of 2:100 presented better adhesion. The conductivity and densification of the film reached the highest at 550 °C. Optimal conductivity of the film with I/Ag ratio of 1:100 and 2:100 was 11.45 and 15.85 mΩ/sq., respectively. In addition, the film adhesion was enhanced by heating. Different trends observed in electrical conductivity and adhesion as functions of temperature is primarily attributed to shrinkage of modified particles. This work suggested that the I/Ag ratio plays a vital effect on the performance of the silver thick film.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Haiyan Liu,Yunfeng Bai,Jun Qin,Haiyan Wang,Yuzhen Wang,Zezhong Chen,Feng Feng
A novel sensitive fluorescence method was developed for adenosine detection by using adenosine aptamer, Exo I and a 2-aminopurine-modified DNA (2-AP probe). 2-AP probe was completely complementary to adenosine aptamer. This strategy took advantage of the high binding affinity of adenosine aptamer and the susceptibility of 2-aminopurine (2-AP) to the local base stacking environment. In the absence of the adenosine, the formation of double-stranded DNA (dsDNA) through the hybridization of the aptamer with the 2-AP probe prevented the digestion of 2-AP probe by Exo I. The fluorescence of 2-AP was significantly quenched due to the base-stacking interaction. In the presence of adenosine, the binding of adenosine with the aptamer prevented the hybridization between aptamer and 2-AP probe, leading to the digestion of 2-AP probe by Exo I and the release of 2-AP. The fluorescence was recovered. A limit of detection of 0.30 μM and a linear range from 10 μM to 600 μM ( R 2 = 0.9933) were achieved by this assay. Moreover, adenosine analogs did not produce any significant change in the fluorescence intensity response, indicating good selectivity of this sensor for adenosine detection. Finally, it was used for the diluted serum sample.
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