ANALYTICAL LETTERS
·
2020
·
IF 1.467
Wanxin Dong,Xinxin Mo,Yu Wang,Qin Lei,He Li
In this work, a novel flexible photoelectrochemical immunosensor is developed to determine cardiac troponin I (cTnI) in serum. The flower-like ZnIn2S4 nanospheres (ZIS), as signal amplification material, were successfully synthesized by a solvothermal method. The ZIS and Bi2Se3 were modified on the indium tin oxide- polyethylene terephthalate (ITO-PET) electrode, which effectively accelerates the electronic transition and improved the photocurrent conversion efficiency (signal on). However, the binding of antigen–antibody proteins blocks the electron transfer, resulting in a greatly reduced photocurrent (signal off). By adding the second antibody modified by CdSe quantum dots (CdSe QDs-Ab2), the photocurrent is increased (signal on). The constructed immunosensor has a linear range from 0.08 to 40 ng/mL with a detection limit of 0.026 ng/mL, which shows excellent selectivity, high sensitivity, and good stability. The photoelectrochemical immunosensor has broad application prospects for the clinical examination of myocardial infarction.
研究领域:
生物医学工程
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临床检验
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DOI
高影响因子
DALTON TRANSACTIONS
·
2020
·Vol. 49
·期 18
·6074-6083
·
IF 4.174
Rongyun Jiang,Jian Yang,Yangqi Meng,Duanting Yan,Chunguang Liu,Changshan Xu,Yuxue Liu
Zn2Ga3−x−yCrxNdyGe0.75O8 (ZGGO:Crx,Ndy) persistent luminescent nanoparticles (PLNPs) (x = 0, 0.02; and y = 0, 0.01, 0.02, 0.03, 0.04) were synthesized via a hydrothermal method in combination with a following heat treatment under vacuum. The effects of Nd3+ concentration on chemical composition, size distribution, luminescence property, the persistent energy transfer from Cr3+ to Nd3+ and afterglow imaging were studied in detail. When the Nd3+ concentration was increased from 0 to 0.04, the particle size increased from 45.4 to 86.2 nm. The afterglow emissions in the NIR-I (696 nm) and NIR-II (1067 nm) regions, which are ascribed to the 2E, 4T2 → 4A2 transitions of Cr3+ and the 4F3/2 → 4I11/2 transition of Nd3+, respectively, were simultaneously acquired after stopping the 635 nm excitation. Among the nanoparticles with different concentrations, ZGGO:Cr0.02,Nd0.02 exhibits the strongest NIR-II afterglow intensity and the corresponding energy transfer efficiency from Cr3+ to Nd3+ is found to be 25.9%. In addition, enhanced X-ray excited afterglow imaging can be observed in Nd3+/Cr3+ codoped nanoparticles dispersed in water, human serum albumin solution and simulated lysosomal environment compared to the Cr3+ singly doped nanoparticles. Renewable NIR afterglow imaging was realized through an X-ray reexcitation strategy. In particular, both the X-ray excitation strategy accompanied by NIR-I afterglow emission and the red light (635 nm) excitation strategy accompanied by NIR-II afterglow emission exhibit high tissue penetration capability. This study provides a further understanding of how to develop a suitable strategy for realizing deep tissue autofluorescence-free bioimaging.
研究领域:
生物医学工程
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材料科学
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光学成像
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DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Qingwei Song,Zixuan Liao,Lin Zheng,Pan Fu,Sihua Qian,Wei Liang,Jianping Zheng,Kaizhe Wang,Yuhui Wang
Fluorescence lateral flow immunoassays (FL-LFIA) have attracted considerable attention in clinical diagnosis due to their outstanding merits of affordable, sensitive, on-site, and quick detection. However, they are still plagued by significant signal interference, such as autofluorescence and scattered light. The development of high-performance and robust phosphors, i.e., label probes featuring with the character of low/no optical background, remains a great challenge. Herein, we report a novel visualized phosphorescence LFIA (Phos-LFIA), where the composite microspheres, i.e., carbon dots (CDs) covalently embedded in dendritic mesoporous silicon nanoparticles (DMSNs), were designed and selected as the report probes. The obtained CDs@DMSNs revealed uniform morphologies and particle sizes, as well as ultralong (lifetime: 1.14 s, visible for over 8 s to naked eyes) room temperature phosphorescence (RTP) in aqueous solution. As competitive nanotags, CDs@DMSNs were designed for an ultralong phosphorescence-based time-gated LFIA for cardiac troponin I (cTnI) without optical interference. The fabricated Phos-LFIA test strips demonstrated zero-background signal and were applied for highly sensitive cTnI detection in both buffer and a complex serum matrix, with corresponding limits of detection (LODs) of 0.19 and 0.21 ng/mL, respectively. For a clinical validation, the proposed Phos-LFIA revealed an excellent clinical analytical performance (sensitivity: 95.45%, specificity: 88.9%, κ value: 0.85), demonstrating its potential for rapid and accurate diagnosis of myocardial infarction. This work provided a promising background-free probe for FL-LFIA, and it would also open an opportunity for developing highly sensitive screening platforms for other targets through modifying different recognition ligands onto CDs@DMSNs.
研究领域:
医学诊断
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生物材料
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临床检验
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DOI
PubMed
高影响因子
ORGANIC ELECTRONICS
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2021
·
IF 3.721
Ting-Hong Huang,Cheng Luo,Dan Zheng
Cationic and neutral mononuclear Cu(I) complexes, [Cu(PPh 3 ) 2 (PmH)]BF 4 (1a), [Cu(DPEphos) (PmH)]BF 4 (2a), [Cu(Xantphos) (PmH)]BF 4 (3a), [Cu(PPh 3 ) 2 (Pm)] (1b), [Cu(DPEphos) (Pm)] (2b) and [Cu(Xantphos) (Pm)] (3b) (PPh 3 = triphenylphosphine , DPEphos = bis(2-diphenylphosphinophenyl)ether, Xantphos = 9, 9-dimethyl-bis(diphenylphosphino)xanthenes, PmH = 2-(pyridin-2-yl)benzimidazole, Pm=(2-(Pyridin-2-yl)benzimidazolate), have been prepared and characterized by IR, 1 H NMR, 13 C NMR, 31 P NMR, XRD, elemental analysis and X-ray crystal structure analysis. The structural analysis shows that each of Cu(I) complexes includes a tetrahedral [Cu(NN) (PP)] + moiety, and temperature variation from 99 K to 298 K leads to the change of bonds lengths, angles and weak interactions. Meanwhile, theoretical calculations indicate that the differences between cationic and neutral Cu(I) complexes affect the composition of HOMO and LUMO orbitals, and the effect of temperature on Mülliken atomic charges is limited. Furthermore, neutral Cu(I) complexes 1b–3b show better luminescence in comparison to cationic Cu(I) complexes 1a-3a at room temperature , and temperature variations from 99 K to 298 K result in changing photoluminescence to some extent, which partly agrees with the related calculation results. In these cationic and neutral Cu(I) complexes, the maximum phosphorescent lifetime and quantum yield reach respectively 137 μs and 42% at room temperature. Moreover, cationic and neutral Cu(I) complexes are utilized to fabricate the monochromatic LEDs, showing favorable electroluminescence with the maximum EQE of 7.10%.
研究领域:
材料化学
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光学
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理论计算
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DOI
高影响因子
JOURNAL OF DISPERSION SCIENCE AND TECHNOLOGY
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2017
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IF 1.591
Lin Yang,Shengfang Li,Chunyan Sun
A new Cs(I) magnetic ion-imprinted polymer (Cs(I)-MIIP) aimed at the selective adsorption and separation of Cs(I) from salt lake brine was prepared. The Fe3O4@SiO2 was used as supporter, Cs(I) as template ion, and carboxymethyl chitosan as functional monomer. The product was characterized by Fourier transform infrared spectra, XRD, energy-dispersive spectrometry, scanning electron microcopy, thermogravimetric analysis, and vibrating sample magnetometer. The adsorption of the Cs(I)-MIIP in solution was investigated, which indicated the maximum adsorption capacity was 36.15 mg·g−1 under the optimum conditions. The pseudo-first-order kinetic model and the Freundlich isotherm model were applied to predict the adsorption process of Cs(I) onto Cs(I)-MIIP. Selectivity experiments showed that the relative selectivity coefficient (k′) were 24.995, 1.73, 1.43, 4.83, and 1.63 to Cs(I)/Li(I), Cs(I)/Na(I), Cs(I)/K(I), Cs(I)/Rb(I), and Cs(I)/Sr(II) binary solutions, higher than those of NIP, respectively. Furthermore, the Cs(I)-MIIP was successfully applied to the enrichment and separation of Cs(I) from the salt lake brine of Qinghai, with satisfactory Cs(I) recovery rates.
研究领域:
材料科学
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环境科学
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DOI
高影响因子
MICROCHIMICA ACTA
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2025
·Vol. 193
·期 1
·15
·
IF 5.3
Zhang Yan,Yan Yuting,Huang Yue
An innovative electrochemical biosensor is proposed for the sensitive detection of ofloxacin (OFL) by integrating exonuclease I (Exo I)–assisted recognition-transduction strategy into the MXene/AuNP-enhanced electrochemical platform. Through ingenious design, the presence of OFL triggers the competitive displacement reaction and enables Exo I to hydrolyze the released single-stranded DNA probe containing the sequence of Pb 2+ -DNAzyme, protecting the signal probes on the electrode surface from DNAzyme cleavage, which eventually brings about a remarkable electrochemical signal positively related to the concentration of OFL. MXene/AuNPs with excellent conductivity, large specific surface area, and good chemical stability are employed as the electrode substrate to efficiently accelerate charge transfer and increase the loading capacity for signal probes, leading to the enhancement of signal output and endowing the biosensor with high sensitivity, rapid response, and fine robustness. Taking advantage of OFL recognition–initiated Exo I hydrolysis to DNAzyme, the electrochemical platform with “signal-off/on” mode can detect OFL with good specificity and reduced background interference. Benefiting from the synergistic effect of the involved sensing elements, the developed electrochemical biosensor achieves a low detection limit of 0.47 pg mL −1 with a broad linear range and exhibits satisfactory detection performance in real food samples, indicating potential practical applicability in food safety monitoring. Graphical
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PubMed
高影响因子
TOXICON
·
2025
·
IF 2.4
Long Xu,Yuzhu Xu,Yiwen Lin,Jingdi Niu,Xinxing Gao,Wanwan Wang,Shanqin Qian
Aristolochic acids (AAs) are a family of nitrophenanthrene carboxylic acids. Some AAs-containing herbs are used as raw materials in medicines, health foods, cosmetics, and dietary supplements. However, aristolochic acid I (AAI) as the main active component of AAs, is nephrotoxic, mutagenic, and carcinogenic to humans. Thus, it is essential to develop effective techniques for determining AAI in relevant products. In this study, an anti-AAI monoclonal antibody (mAb) with high sensitivity was generated. Based on the mAb, a chemiluminescence enzyme immunoassay (CLEIA) was developed, exhibiting an IC 50 of 20 ng/mL and a linear range from 2.5 ng/mL to 250 ng/mL. The limit of detection (LOD) of the CLEIA was 1.96 ng/mL. The recovery rates of AAI in spiked patent medicines and cosmetics ranged from 79.7 % to 121.7 % with coefficient of variations (CVs) ranging from 1.2 % to 13.6 %. A good correlation coefficient of 0.9591 was observed between CLEIA and high-performance liquid chromatography (HPLC) results, indicating that the developed CLEIA is accurate and reliable for the determination of AAI in patent medicines and cosmetics.
研究领域:
医学
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药学
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毒理学
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DOI
PubMed
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2022
·
IF 4.326
Xiaoying Gao,Yunjia Liu,Ke Zhang,Jinlan Weng,Rongqing Chen,Xiaoyi Zhang,Zhipeng Wang,Shenghong Yang,Jian Liu
External light stimuli strategy under conventional conditions achieves a sustainable future due to its controllable spatial and temporal precision coupled with environmental friendliness. Herein, atomic Cu(I)-carbon nitride (Cu-CN) was synthesized and served as specific light-stimuli oxidase mimics for total antioxidant capacity (TAC) assay. Singlet oxygen (1O2) and hydroxyl radical (·OH) are the main intermediates, which can oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) into its blue oxidant and therefore improve photothermal signal generation. Based on the intrinsic light responsiveness and photothermal conversion property, we established a colorimetric and photothermal dual-mode sensing platform. The proposed dual-mode assay presented a specific assay of ascorbic acid with LOD of 0.28 μM and 0.15 μM, respectively. This platform was further successfully applied to assess TAC of fruits and beverages. It is believed that the intelligently modulated activity and dual-mode detection function of such atomic enzyme mimics can offer a promising strategy for other analytical assays in high repeatability and reproducibility.
研究领域:
生物化学
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分析化学
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DOI
高影响因子
Journal of Materials Chemistry A
·
2018
·Vol. 6
·期 19
·8930-8939
·
IF 9.931
Yu-Xia Li,Shuai-Shuai Li,Ding-Ming Xue,Xiao-Qin Liu,Meng-Meng Jin,Lin-Bing Sun
Cu(I)-containing materials have great potential in various applications such as in CO adsorption; however, development of an efficient and controllable method to produce Cu(I) sites remains a significant challenge; herein, a two-step double-solvent (DS) strategy is reported for the first time to fabricate Cu(I) sites in a representative metal–organic framework, MIL-101(Cr); this strategy ensures that both introduction of the Cu(II) precursor and its reduction to Cu(I) occur inside the pores and significantly minimizes the aggregation of Cu species. This is difficult to realize through conventional methods used for Cu(II) introduction (wet impregnation) or reduction (liquid-phase reduction). The two-step DS strategy involves selective reduction of Cu(II) to form Cu(I) without the formation of any Cu(0). The obtained Cu(I)-containing materials exhibit an excellent CO adsorption capacity (up to 2.42 mmol g−1) at 298 K and 1 bar, much better than that of the benchmark adsorbents including CuCl/γ-Al2O3 (1.0 mmol g−1), CuCl/MCM-41 (0.57 mmol g−1), and CuZSM-5 (0.11 mmol g−1).
研究领域:
材料科学
·
化学工程
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DOI
高影响因子
FITOTERAPIA
·
2024
·
IF 2.5
Lin Zhu,Yongle Yu,Youfu Ma,Yenong Shi,Jamal Alzobair Hammad Kowah,Lisheng Wang,Mingqing Yuan,Xu Liu
Sophoridine, which is derived from the Leguminous plant Sophora alopecuroides L., has certain pharmacological activity as a new anticancer drug. Herein, a series of novel N-substituted sophoridine derivatives was designed, synthesized and evaluated with anticancer activity. Through QSAR prediction models, it was discovered that the introduction of a benzene ring as a main pharmacophore and reintroduced into a benzene in para position on the phenyl ring in the novel sophoridine derivatives improved the anticancer activity effectively. In vitro, 28 novel compounds were evaluated for anticancer activity against four human tumor cell lines (A549, CNE-2, HepG-2, and HEC-1-B). In particular, Compound 26 exhibited remarkable inhibitory effects, with an IC 50 value of 15.6 μM against HepG-2 cells, surpassing cis-Dichlorodiamineplatinum (II). Molecular docking studies verified that the derivatives exhibit stronger binding affinity with DNA topoisomerase I compared to sophoridine. In addition, 26 demonstrated significant inhibition of DNA Topoisomerase I and could arrest cells in G 0 /G 1 phase. This study provides valuable insights into the design and synthesis of N-substituted sophoridine derivatives with anticancer activity.
研究领域:
药物化学
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肿瘤学
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计算生物学
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PubMed
高影响因子
MOLECULAR AND CELLULAR PROBES
·
2016
·
IF 1.565
Xiaoqian Liu,Jingjing Wang,Yi Chu,Xiaoying Zhou
Dipeptidyl peptidase I (DPPI) is a lysosomal cysteine protease and derived from immune granule cells . It has been suggested playing an important role in the development of rheumatoid arthritis . In this study, a coumarin based fluorescent probe (GF-AFC) was designed and synthesized to evaluate DPPI activity in serum or tissue homogenates of collagen-induced arthritis (CIA) rats, an inflammatory arthropathy model. It was revealed that the fluorescent intensity was significantly increased in a very short time after specific substrate GF-AFC reacted with the DPPI. The fluorophore (AFC) was released to shine after the cleavage reaction which was examined by 19 F NMR spectroscopy. It has been shown that DPPI hydrolyzed the GF-AFC in a robust, linear, and time dependent manner at a significant high rate. A serum-based DPPI activity assay was validated by spiking and gradient dilution methods, there were no interferences or auto-fluorescence observed. The Coefficient of Variance calculated for serum-based DPPI activity assays indicates the good reproducibility. The good correlation has been seen between serum DPPI levels and the severity of arthritis during RA development in CIA rats. Our study has demonstrated a new serum based diagnostic assay for detecting DPPI activity using coumarin conjugated fluorescent (GF-AFC) as a substrate. The successful implementation of the case would provide beneficial experience in rheumatoid arthritis research.
研究领域:
免疫学
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医学
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药理学
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DOI
PubMed
高影响因子
Biosensors-Basel
·
2022
·Vol. 12
·期 12
·1108
·
IF 5.743
Ding Wang,Yiru Zhao,Shen Zhang,Liping Bao,Huijun Li,Jingcheng Xu,Bin He,Xumin Hou
Rapid and accurate detection of acute myocardial infarction can improve patients’ chances of survival. Cardiac troponin I (cTn I) is an important diagnostic biomarker for acute myocardial infarction. However, current immunoassays are insufficient to accurately measure cTn I, as they have limited detection sensitivity and are time-consuming. Surface-enhanced Raman scattering (SERS) is a brilliant fingerprints diagnostic technique characterised by ultrasensitivity, fast response, and qualitative and quantitative analysis capabilities. In this study, reporter molecules (4-Mercaptobenzoic acid, 4-MBA) embedded[email protected]core-shell nanospheres as SERS nanotags were prepared for the detection of cTn I. As the Raman reporters were embedded between the core and the shell, they could be protected from the external environment and nanoparticle aggregation. Excellent SERS performances were obtained due to the enhanced local electromagnetic field in the gap of core and shell metals. In a standard phosphate buffered saline (PBS) environment, the limit of detection for cTn I was 0.0086 ng mL−1(8.6 ppt) with a good linear relationship. The excellent Raman detection performance was attributed to the localized surface plasmon resonance effect and strong electromagnetic field enhancement effect produced by the gap between the Au core and the Ag shell. The SERS nanotags we prepared were facile to synthesize, and the analysis procedure could be completed quickly (15 min), which made the detection of cTn I faster. Therefore, the proposed SERS nanotags have significant potential to be a faster and more accurate tool for acute myocardial infarction diagnostics.Keywords:[email protected]nanospheres;GERTs;SERS;cTn I detection;AMI
研究领域:
医学诊断
·
纳米技术
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生物化学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）