EUROPEAN JOURNAL OF PHARMACOLOGY
·
2025
·
IF 4.7
Guo-Xuan Liu,Fan Diao,Guang Lu,Qiang Zheng,Bin Fu,Hua-Chen Jiao,Ke-Zhou Wang,Dong-Hai Liu
Alectinib is the first-line therapy for anaplastic lymphoma kinase rearranged non-small cell lung cancer globally. Sinus bradycardia, as the major adverse cardiac events of alectinib, still widely impact patient’s quality of life. However, its underlying mechanism remains elusive. The aim of this study was to reveal the pathogenesis of the alectinib induced sinus bradycardia (AISB) in a rat model, including the electrophysiology alterations and the molecular mechanism. SD rats were administered alectinib (10 mg / kg / day) by gavage for 7 to 10 days to mimic the clinical AISB. 3-days alectinib treatment did not change heart rate and sinus node recovery time (SNRT) as assessed through in vivo electrophysiology study. Also, alectinib didn’t influence automaticity in isolated heart or single sinus node cardiomyocytes, indicating alectinib cannot decrease sinus node function rapidly. The decreased heart rate and prolonged SNRT was found after 7-days alectinib treatment. The inducibility of atrial fibrillation was not affected under the same condition. The RNA-seq assay revealed the transcriptomic alterations in sinus node of alectinib treated rats, and the dysregulation of genes in cardiac function were observed. The decreased expression of L-type calcium channel Cacna1d was confirmed among the channel candidates identified from RNA-seq assay. Subsequently, the patch-clamp test revealed the reduction in the corresponding L-type calcium current density in 7-days alectinib treated rats. These findings revealed that the AISB was caused by the reduction in Cacna1d expression which resulted in the electro-dysfunction mediated by the suppressed I CaL .
研究领域:
肿瘤学
·
心脏病学
·
分子生物学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2015
·Vol. 7
·期 6
·2855-2861
·
IF 1.821
Zhuo Ye,Lingxi Yang,Jianguo Wen,Baoxian Ye
A novel voltammetric sensor, based on a single-walled carbon nanotube (SWNT) composite poly(L-serine) film modified electrode, was fabricated by simple dipping–drying and electrodeposition methods and used for highly sensitive determination of natamycin. At this sensor, the electrochemical response of natamycin was improved greatly due to the synergistic effect of SWNTs and poly(L-serine). A new voltammetric method for the determination of natamycin was proposed with a detection linear range of 6 × 10−8 mol L−1 to 6 × 10−6 mol L−1 and a detection limit of 4 × 10−8 mol L−1. Furthermore, the electrode reaction of natamycin was investigated in detail and some dynamic parameters were calculated for the first time. Using the present method, the residual quantity of natamycin in available claret and beverage samples was evaluated with good satisfactory recovery.
研究领域:
分析化学
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食品科学
·
电化学
✓ 已验证
DOI
高影响因子
Journal of Macromolecular Science Part A-Pure and Applied Chemistry
·
2013
·
IF 0.807
Shi-Ping Zhan,Xing Huang,Qi-Cheng Zhao,Li Chen,Wei-Min Hou,Song Zhang,Jin-Jun Deng
Dispersion polymerization of lactides and lactones has been studied and has received a great deal of attention used to prepare biodegradable polymers in supercritical carbon dioxide (ScCO2). The triblock copolymers of poly(ϵ-caprolactone) PCL-polydimethylsiloxane (PDMS)-poly(ϵ-caprolactone) PCL by the feed ratio of 1:2:1 and 1:3:1, respectively were synthesized to be used as the stabilizers in the dispersion polymerization of L-lactide. The fine poly(L-lactide) (PLLA) powders were obtained at the concentrations of 3% to 5%w/w (stabilizer/monomer). The results demonstrated that the triblock copolymers were the effective stabilizers for the dispersion polymerization of L-lactide. It was also found that the cooling process had a significant effect on the particle size of the fine powders. In order to obtain the best PLLA powders, it would be necessary to use the cooling process with stirring.
研究领域:
高分子科学
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化学工程
·
材料科学
·
绿色化学
✓ 已验证
DOI
高影响因子
Analytical Methods
·
2025
·
IF 2.6
Changqiu Ma,Daheng Jiang,Linghui Zeng,Lixin Zhu,Xiaoliang Xu
Conventional photoelectrochemical (PEC) biosensors based on ZnO and TiO2 face challenges in applications in vivo due to the poor penetration of excitation light through the skin. To address this limitation, we designed a novel near-infrared (NIR)-driven FTO@UC@Cu2O@TiO2 heterojunction biosensor for subcutaneous L-cysteine detection. NIR light penetrates the skin and excites the upconversion material (called UC for short, NaYF4:Er, Yb), which emits visible light at 540 nm. This emission activates the Cu2O semiconductor to generate photocurrent. The Cu2O@TiO2 heterojunction facilitates charge carrier separation and suppresses electron-hole recombination. The biosensor demonstrates stable, sensitive, and reproducible detection of L-cysteine over a range of 10 μM to 10 pM, with a detection limit of 5.2 pM. Notably, it also enables subcutaneous detection covering a range from 10 μM to 100 pM, with a detection limit of 74 pM. These results provide a promising strategy for real-time in situ monitoring of L-cysteine in clinical applications.
研究领域:
生物传感器
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纳米技术
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分析化学
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生物医学工程
·
光化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED CLAY SCIENCE
·
2021
·
IF 5.467
Lei Wang,Haris Muhammad,Minwang Laipan,Xiaohu Fan,Junkang Guo,Yongtao Li
The demand for clean water and soil drives high efficiency, less expensive, and eco-friendly clay-composites as adsorbents for remediation of heavy metals polluted environment. We have developed a new water remediation technique by using an organic-inorganic composite, L-cysteine doped polypyrrole modified bentonite (L-cys/PPy/BT) for removal of Cr(VI) and Mo(VI). The L-cys/PPy/BT showed maximum adsorption capacities of 318.5 and 65.4 mg/g for Cr(VI) and Mo(VI) respectively. For Cr(VI) and Mo(VI) the sorption process on L-cys/PPy/BT followed the Freundlich and Langmuir isotherm models, respectively. Furthermore, the pseudo-second-order kinetic model described the adsorption behavior of Cr(VI) and Mo(VI). Moreover, the thermodynamic parameters indicated that sorption processes were endothermic and spontaneous. Mechanistic assessment revealed that adsorption of Mo(VI) on L-cys/PPy/BT could be attributed to chelation, electrostatic attraction and ion-exchange of Mo(VI) with the imine/amine functional groups in the material's matrix , while electrostatic attraction, ion exchange, chelation and redox reaction played a significant role in the removal of Cr(VI). This research provides a new approach for the use of clay materials as potential sorbents for removal of heavy metal ions in contaminated water .
研究领域:
环境科学
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材料科学
✓ 已验证
DOI
高影响因子
CHEMOSPHERE
·
2022
·
IF 8.943
Boyin Yan,Songxue Wang,Zhiquan Liu,Da Wang,Wenxin Shi,Fuyi Cui
In this work, the UV 254 /H 2 O 2 process was utilized to remove β-N-methylamino- l -alanine (BMAA), a kind of cyanobacteria neurotoxin, and the influence of reaction parameters and environmental factors on the degradation of BMAA has been systematically investigated. The results showed that BMAA could be effectively removed in the UV 254 /H 2 O 2 system compared to UV or H 2 O 2 alone and OH was confirmed as the main ROS to degrade BMAA. The degradation rate of BMAA increased first and then decreased with the increase of pH and the maximum k obs was 0.1545 min −1 obtained at pH 9. The removal of BMAA in the UV 254 /H 2 O 2 system was inhibited in actual water, while the degradation rate of BMAA in actual water could still exceed 90% by appropriately extending the reaction time. The decrease in the degradation efficiency of BMAA in actual water was primarily due to the ultraviolet light absorption and competition effects of NOM, and anions (Cl − and HCO 3 − ) would also inhibit the degradation of BMAA. Five by-products ([M − H] - = 118, 103, 88, 87 and 59) were identified in this study and the degradation pathways of BMAA were proposed. The production of by-products was attributed to the fracture of the C–N bond and hydroxylation reaction. This study is worthwhile to deepen the understanding of the degradation mechanism of BMAA in the UV 254 /H 2 O 2 system.
研究领域:
环境科学
·
毒理学
✓ 已验证
DOI
高影响因子
MACROMOLECULAR BIOSCIENCE
·
2013
·Vol. 14
·期 2
·280-288
·
IF 3.742
Feifei Li,Bin Chen,Zhaohua Ping,Xiangxiang Jia,Xin Guo,Longqiang Xiao,Lijian Liu
Lipophilic 6- O -poly ( ϵ -caprolactone)- L -ascorbic acid (AA-6-PCL) is synthesized through ROP of ϵ -caprolactone (CL). The number of repeating CL units in the polymer chain varies from 6 to 19. AA-6-PCL loaded supramolecular polymer micelles (SMPMs) are constructed with β-cyclodextrin (β-CD) and PCL as blocks. Transmission electron microscopy images show a nanospheric morphology of the micelles with a size range of 43.3 ± 5.0 nm. The drug loading contents are 22.53–39.23% for AA-6-PCL. AA-6-PCL exhibits high radical scavenging capacity (93.96–96.73%) and efficient scavenging potency, and a cytotoxicity study proves the excellent cytocompatibility of AA-6-PCL loaded β-CD/PCL SMPMs, which altogether herald their potential application in the study of the induced pluripotent stem cells.
研究领域:
材料科学
·
药学
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
Catalysts
·
2022
·Vol. 12
·期 9
·971
·
IF 4.501
Zewang Guo,Denghui Chen,Qi Xiong,Miao Liang,Pengfei Li,Zehui Gong,Junzhi Qiu,Liaoyuan Zhang
Leucine dehydrogenase (LeuDH) has emerged as the most promising biocatalyst for L-tert-leucine (L-Tle) production via asymmetric reduction in trimethylpyruvate (TMP). In this study, a new LeuDH namedPbLeuDH from marinePseudomonas balearicawas heterologously over-expressed inEscherichia coli, followed by purification and characterization.PbLeuDH possessed a broad substrate scope, displaying activities toward numerous L-amino acids andα-keto acids. Notably, compared with those reported LeuDHs,PbLeuDH exhibited excellent catalytic efficiency for TMP with aKmvalue of 4.92 mM and akcat/Kmvalue of 24.49 s−1mM−1. Subsequently, L-Tle efficient production was implemented from TMP by whole-cell biocatalysis using recombinantE. colias a catalyst, which co-expressedPbLeuDH and glucose dehydrogenase (GDH). Ultimately, using a fed-batch feeding strategy, 273 mM (35.8 g L−1) L-Tle was achieved with a 96.1% yield and 2.39 g L−1h−1productivity. In summary, our research provides a competitive biocatalyst for L-Tle green biosynthesis and lays a solid foundation for the realization of large-scale L-Tle industrial production.Keywords:leucine dehydrogenase;L-tert-leucine;trimethylpyruvate;reductive amination;whole-cell biocatalysis
研究领域:
生物化学
·
工业微生物学
✓ 已验证
DOI
高影响因子
JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS
·
2015
·
IF 2.352
Yuan Yao,Haifeng Liu,Xili Ding,Xiaohui Jing,Xianghui Gong,Gang Zhou,Yubo Fan
In recent years, silk fibroin has become one of the most promising tissue engineering materials because of its excellent cytocompatibility. Poly(l-lactide-co-ε-caprolactone), the copolymer of poly(l-lactide) and poly(ε-caprolactone), possesses good mechanical properties, and its degradation rates can be manipulated by varying the ratio of the constituent polymers. In this study, in order to combine their respective characteristics, silk fibroin/poly(l-lactide-co-ε-caprolactone) nanofibrous membranes were fabricated through electrospinning with different mass ratios of 100:0, 75:25, 50:50, 25:75, and 0:100. The surface properties, thermodynamic properties, mechanical properties, and cytocompatibility of silk fibroin/poly(l-lactide-co-ε-caprolactone) blended membranes were evaluated, and an optimal blending ratio was identified. The results showed that the silk fibroin/poly(l-lactide-co-ε-caprolactone) blended membranes containing 75% of silk fibroin and 25% of poly(l-lactide-co-ε-caprolactone) achieved the most improved performances compared with the single-component membranes or the blended membranes with other mixing ratios. The results from this study indicated that 75/25 silk fibroin/poly(l-lactide-co-ε-caprolactone) blended membranes which combined the advantages of poly(l-lactide-co-ε-caprolactone) and silk fibroin might be a suitable candidate material for use in tissue engineering.
研究领域:
生物材料
·
组织工程
·
材料科学
·
生物医学工程
✓ 已验证
DOI
高影响因子
Research
·
2023
·
IF 11
Nian Liu,Lihui Chen,Mingjie Yan,Qian Tao,Jie Wu,Jing Chen,Xiang Chen,Wei Zhang,Cong Peng
Natural killer (NK) cells, as key immune cells, play essential roles in tumor cell immune escape and immunotherapy. Accumulating evidence has demonstrated that the gut microbiota community affects the efficacy of anti-PD1 immunotherapy and that remodeling the gut microbiota is a promising strategy to enhance anti-PD1 immunotherapy responsiveness in advanced melanoma patients; however, the details of the mechanism remain elusive. In this study, we found that Eubacterium rectale was significantly enriched in melanoma patients who responded to anti-PD1 immunotherapy and that a high E. rectale abundance was related to longer survival in melanoma patients. Furthermore, administration of E. rectale remarkably improved the efficacy of anti-PD1 therapy and increased the overall survival of tumor-bearing mice; moreover, application of E. rectale led to a significant accumulation of NK cells in the tumor microenvironment. Interestingly, conditioned medium isolated from an E. rectale culture system dramatically enhanced NK cell function. Gas chromatography–mass spectrometry/ultrahigh performance liquid chromatography–tandem mass spectrometry-based metabolomic analysis showed that l-serine production was significantly decreased in the E. rectale group; moreover, administration of an l-serine synthesis inhibitor dramatically increased NK cell activation, which enhanced anti-PD1 immunotherapy effects. Mechanistically, supplementation with l-serine or application of an l-serine synthesis inhibitor affected NK cell activation through Fos/Fosl. In summary, our findings reveal the role of bacteria-modulated serine metabolic signaling in NK cell activation and provide a novel therapeutic strategy to improve the efficacy of anti-PD1 immunotherapy in melanoma.
研究领域:
免疫学
·
肿瘤学
·
微生物学
✓ 已验证
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Yanan Deng,Zihao Zhang,Yuanhao Pang,Xueying Zhou,Yu Wang,Yun Zhang,Yali Yuan
The poly-L-cysteine modified Au nanoparticles (Au@p-L-Cys) were constructed on electrode surface as a highly efficient chiral interface for tryptophan (Trp) enantiomers recognition via one step electropolymerization. With the aid of Cu 2+ , L-Cys residues and D-Trp target formed a sandwich complex D-Trp-Cu 2+ -L-Cys, while L-Trp was unable to form such complex due to the steric hindrance provided by the chiral interface, which was confirmed by the electrochemical and SEM results. With the introduction of ferricyanide probe, D-Trp produced significant current decrease while L-Trp produced a slight current increase, which implied the successful enantioselective recognition of Trp enantiomers (specifically D-Trp) in the true sense. This novel sensor showed a surprisingly wide linear range toward D-Trp of 6 × 10 −7 M to 1 × 10 −2 M, with a detection limit as low as 75 nM (S/N = 3). Moreover, the exclusive enantioselectivity toward D-Trp was discovered since other amino acids showed negligible interference to detection of D-Trp. The recovery of D-Trp in human serum was between 91.30 and 109.3%, which further verified the satisfying specificity and practicality of the proposed strategy. The coordination thermodynamics by UV–Vis spectroscopy and DFT simulation were also used to investigate the enantioselective mechanism. These results highlight the great potential of using Au@p-L-Cys to construct chiral interface for enantiomers recognition and hold the promise of practical application of electrochemical chiral sensors in fields like pharmaceutics and bioanalysis .
研究领域:
生物化学
·
分析化学
·
医药科学
✓ 已验证
DOI
高影响因子
JOURNAL OF THE EUROPEAN CERAMIC SOCIETY
·
2024
·
IF 5.8
Yuan-Bin Chen,Siyi Xiong
This article explores the microwave dielectric properties of (La 1-x Nd x ) 2 (Zr 0.96 Ti 0.04 ) 3 (MoO 4 ) 9 (x=0.1,0.3,0.5, and 0.7) ceramics prepared by solid-state reaction. Utilizing the Rietveld refinement technique in XRD, the sample is identified as a trigonal crystal system belonging to the R 3 ¯ c space group. SEM analysis indicates that relative density has a significant impact on the ε r and Q×f of ceramics. The Q×f values of ceramics are closely related to the FWHM of the Raman spectra. At 750℃, (La 0.5 Nd 0.5 ) 2 (Zr 0.96 Ti 0.04 ) 3 (MoO 4 ) 9 ceramic exhibited excellent dielectric properties: ε r = 10.4 (±0.08), Q×f = 149,300 (±3211) GHz, τ f = −34.8 (±0.7) ppm/℃. To gain a deeper understanding of the intrinsic mechanisms governing the microwave dielectric properties of ceramics, analysis based on the P-V-L theory reveals that the La(Nd)-O bond and Mo-O bond contribute significantly to ε r and Q×f, respectively.
研究领域:
材料科学
·
陶瓷学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）