JOURNAL OF MOLECULAR STRUCTURE
·
2024
·
IF 4
Shuangbao Li,Shuaibing Ma,Jian Li,Yongbo Yu,Zechao Wu,Yuteng Zhang,Li Jin,Julun Zong,Jianpo Zhang
Malachite green (MG), a cost-effective triphenylmethane dye known for its insecticidal and germicidal properties in aquaculture, unfortunately poses significant environmental and human health-related risks due to its mutagenic, carcinogenic, and teratogenic effects. Herein, a novel diamine-functionalized zirconium-based metal organic cage (Zr-MOC), abbreviated as ZrT-1-(NH 2 ) 2 , was synthesized through microwave-assisted method, serving as a ratiometric fluorescent sensor for the precise detection of MG in aquaculture water. When the pH value was adjusted to 3.0, the probe solution exhibited two distinct characteristic emission bands centered at 454 nm and 582 nm, upon excitation at 365 nm. With the addition of MG, the emission intensity at 582 nm was significantly quenched, whereas the fluorescence intensity at 454 nm was gradually increased. The related experimental data and DFT calculations revealed that the quenching process is predominantly governed by the inner filter effect and static quenching mechanisms. Notably, the ratiometric fluorescence probe exhibited a good linear correlation with the MG concentration within the range of 1.37–17.81 nM, boasting a remarkably low detection limit of 1.05 nM. Furthermore, the proposed ZrT-1-(NH 2 ) 2 ratiometric fluorescence probe features high recoveries (95.37–104.93 %) and low relative standard deviations (RSD<3.5 %) for the detection of trace MG in aquaculture water, indicating its feasibility and practicality for MG detection.
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Jiuhe Gao,Xiaohan Zhao,Yuhong He,Wenxiao Xu,Yongzhi Zhou,Xiao Feng,Guangyun He,Tian Zhong,Ye Peng,Ying Xiao,Xi Yu
Crystal violet (CV) and malachite green (MG) are antibiotics used in aquaculture, regulated due to their teratogenicity and potential carcinogenic risk. Detecting them in seafood is challenging due to low concentrations and complex matrices, especially fat interference. In this study, low-temperature clean-up removed fat interference, and magnetic nanoparticles preconcentrated the target analytes, achieving high sensitivity, low cost, and easy operation. The proposed method achieved detection limits of 0.18 ng g −1 and 0.54 ng g −1 for CV and MG, respectively, with quantification limits of 0.21 ng g −1 and 0.60 ng g −1 . The intra- and inter-day relative standard deviations were 1.45 %–4.81 % and 4.53 %–7.60 %, respectively, indicating good reproducibility. Recovery rates were 83.19 %–112.88 %, with stable adsorption capacity after 25 cycles. This method offers an ideal solution for rapid screening of CV and MG in seafood for batch samples in markets, stock houses, and on-site situations, adaptable to various analytes and matrices.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2026
·
IF 4.8
Ting Ma,Jingran Sun,Mingzhu Liu,Zunquan Zhao,Jialei Bai,Xiaoyong Jin,Jin Wu,Yanjun Fang
There is a need for sensitive, rapid, and cost-effective methods to detect butyrylcholinesterase (BChE) and malachite green (MG) in human serum and aquatic products, respectively. Therefore, a dual-mode fluorescence sensing and smartphone-based colorimetric approach was established to detect BChE and MG using manganese dioxide nanosheets (MnO 2 NS) and nitrogen-doped molybdenum oxide quantum dots (N-MoOx QDs). MnO 2 NS catalyses the oxidation of o -phenylenediamine (OPD) to form a yellow fluorescence product, oxOPD, which quenches the blue fluorescence of N-MoOx QDs. The presence of BChE hydrolyses butyrylthiocholine iodide (IBTCh), generating thiocholine that decomposes MnO 2 NS, restoring the blue fluorescence of N-MoOx QDs, and reducing the yellow fluorescence of oxOPD. MG inhibits BChE activity, enhancing N-MoOx QD quenching due to increased oxOPD concentration. Preliminary selectivity assessment indicated that, among the limited number of substances tested in this study, the system showed a pronounced response to MG. Additionally, fluorescence colour changes were captured using a smartphone. This dual-mode method is accurate, convenient, low-cost, and rapid for the detection of BChE in human serum and MG in aquatic products.
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高影响因子
Scientific Reports
·
2026
·
IF 4.9
Luo Yan,Cheng Fangfang,Han Lin,Sun Zhiwang,Wang Yingxu,Zhong Hong,Wang Shifeng
Industrial wastewater causes serious water pollution, so it is urgent to remove toxic dyes like malachite green (MG). In this study, a 0D/2D S-scheme heterojunction was constructed by anchoring CeO 2 nanocubes onto SnSe 2 hexagonal nanosheets via a precisely controlled two-step hydrothermal strategy. In-situ electron paramagnetic resonance (EPR) analysis corroborates that illumination triggers the proliferation of surface oxygen vacancies. These photo-induced oxygen vacancies (OVs, oxygen vacancies) empower the CeO 2 /SnSe 2 heterostructure to sustain exceptional catalytic activity over five consecutive cycles. Moreover, the optimized 0.2 g CeO 2 /SnSe 2 composite exhibits a remarkable degradation efficiency of 100% for MG within 30 min. Such performance enhancement is attributed to the synergy between the 0D/2D dimensional matching and a potent internal electric field (IEF), which triggers an S-scheme charge transfer mechanism to preserve high redox potential while accelerating carrier separation. This work provides a sophisticated blueprint for designing dimension-matched S-scheme heterojunctions for high-performance environmental remediation.
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CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 13.2
Jia-Ming Zhao,Qian Zhang,Ying-Tao Su,Xiao-Man Huang,Bing Li
The high-value use of waste biomass and plastics is crucial for green development and environmental protection. Herein, an efficient hydrochar material (BPS-Na) was synthesized via co-hydrothermal carbonization (co-HTC) of bamboo powder, polyvinyl chloride (PVC), and ammonium persulfate (APS) followed by NaOH activation, and applied to uptake malachite green (MG) from water solutions. The focus was on elucidating the function of APS in modulating the physicochemical characteristics and removal performance of the resulting hydrochar. Compared with the hydrochar (BP-Na) prepared without adding APS, the incorporation of APS slightly enlarged the specific surface area (11.10 vs. 10.17 m 2 ·g −1 ) and total pore volume (0.014 vs. 0.016 cm 3 ·g −1 ), but significantly enhanced the oxygen content from 25.75% to 32.87% and mesopore volume, while reducing the carbon content from 62.53% to 52.35%. Adsorption experiments revealed that the uptake of MG by both BP-Na and BPS-Na followed a monolayer chemisorption mechanism and was endothermic. Notably, the removal ability of BPS-Na at 298 K significantly exceeded that of BP-Na (1181.69 vs. 348.52 mg·g −1 ) and many previously reported hydrochar-based adsorbents. This is mainly ascribed to the enriched O-containing functional groups, particularly carboxylate groups, on BPS-Na, which has been confirmed by XPS and FTIR analyses. Furthermore, the effective adsorption of MG by both BP-Na and BPS-Na is achieved through multiple mechanisms, including hydrogen bonding, electrostatic interactions, ion exchange, and π-π stacking interactions. This work demonstrates a novel and effective APS-assisted co-HTC approach that not only enables the synergistic valorization of waste biomass and PVC but also provides a potent method for tailoring surface chemistry to achieve superior performance in cationic dye removal, advancing beyond conventional co-HTC systems.
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NEW JOURNAL OF CHEMISTRY
·
2023
·Vol. 47
·期 34
·16022-16029
·
IF 3.3
Xingguo Deng,Jingchao Li,Bo Zhao,Zhihua Li
In this study, a silver metal–organic framework (Ag-MOF) was immobilized on graphene oxide (GO) sheets via an ultrasonication process. The Ag-MOF@GO composite showed enhanced physicochemical properties, including an improved surface area and lower agglomeration, as confirmed using characterization techniques such as X-ray diffraction (XRD), the Brunauer–Emmett–Teller (BET) method, and scanning electron microscopy (SEM). Malachite green (MG) is a typical cationic dye, which can be toxic, cause environmental damage and threaten human health. Therefore, the adsorption capacities of Ag-MOF and Ag-MOF@GO for the MG dye were also evaluated at room temperature. Kinetic analysis revealed a significant increase in the adsorption capacity and rate of adsorption of Ag-MOF@GO compared with Ag-MOF. The addition of GO increases the surface area of Ag-MOF@GO and reduces the agglomeration effect, resulting in an improved adsorption capacity. Ag-MOF@GO showed a greater than 90% regenerability after three cycles.
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MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Ji Chen,Lumiao Liao,Hongming Lu,Yuting Hu,Huilan Yang,Danping Zhu,Jinlin Wu,Xi Mei
An AND logic fluorescent chip is developed for dual-miRNA detection in diabetes mellitus with Alzheimer's disease. • Self-primer cyclic amplification and dual rolling circle amplification enable ultrasensitive miRNA analysis. • Split malachite green aptamer transcription allows label-free, catalytically amplified fluorescence signal generation. • The chip achieves 0.15 fM LOD for both miRNAs and excellent specificity against mismatched sequences. • Clinical serum analysis shows 87.5% sensitivity for DMAD diagnosis, outperforming single-miRNA RT-qPCR. Early diagnosis of Diabetes Mellitus complicated with Alzheimer's Disease (DMAD) remains a major clinical challenge, and reliable detection of multiple microRNA (miRNA) biomarkers holds great promise for improving diagnostic accuracy. Herein, we report a split malachite green (MG) aptamer transcription-based fluorescent platform that operates as an “AND” logic gate for simultaneous ultrasensitive detection of two DMAD-associated miRNAs, miRNA-34a and miRNA-132. The platform integrates self-primer cyclic amplification with surface-anchored dual rolling circle transcription (RCT). Only when both target miRNAs are present, a target-recycling process releases Y-shaped probes, which subsequently initiate dual RCT to produce long DNA products containing tandem repeats of split MG aptamer sequences. These two products assemble into an intact MG aptamer, catalytically generating a strong fluorescence signal. The assay achieves a limit of detection as low as 0.15 fM for both miRNAs, with excellent specificity against mismatched and non-target sequences. The platform exhibits high stability (recoveries of 94.1–99.5% in spiked serum) and good reproducibility (coefficients of variation of 4.12% and 5.32% at 100 fM and 10 pM, respectively). In 30 clinical serum samples, the method correlates strongly with RT-qPCR and distinguishes DMAD patients from healthy controls with 87.5% sensitivity, significantly outperforming single-miRNA RT-qPCR (62.5% sensitivity). This “AND” logic-based split aptamer transcription platform provides a sensitive, specific, robust, and user-friendly tool for multiplex miRNA analysis, offering great potential for early DMAD diagnosis and broader applications in complex diseases. Download: Download high-res image (94KB) Download: Download full-size image
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FOOD CHEMISTRY
·
2023
·
IF 8.8
Junya Song,Beibei Zhao,Yiren Wang,Xinfang Liu,Zheng Cheng,Xiaoyu Zhang,Xun Feng
In this study, a new Tb-MOF fluorescence probe was developed for the detection of malachite green (MG) in real aquatic products. Fluorescence sensing experiments revealed that MG can effectively quench the green fluorescence of Tb-MOF suspensions, and the detection process exhibits the advantages of high sensitivity, a wide linear range (0–80 μM), a low detection limit (10.8 nM) and a rapid response time. Selective detection of MG is achieved primarily through fluorescence resonance energy transfer (FRET) and photoinduced electron transfer (PET) mechanisms. Furthermore, a smartphone-assisted Tb-MOF-based agar slice detection platform was constructed for the visual and quantitative detection of MG. Additionally, the on-site detection of MG in crucian and shrimp samples was accomplished with high recoveries (99.8 %-107.99 %) and low relative standard deviations (RSD < 2.2 %). This developed detection platform introduced a low-cost, portable and user-friendly approach for MG detection.
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Journal of Analysis and Testing
·
2020
·Vol. 4
·期 4
·264-272
·
IF 0
Jiang Li-Ping,Li Na,Liu Lin-Qi,Zheng Xian,Du Fu-You,Ruan Gui-Hua
Abstract Polymerized high internal phase emulsion (polyHIPE) monoliths were prepared and applied as adsorbent materials for solid-phase extraction (SPE) of malachite green (MG) and leucomalachite green (LMG) from water samples. The polyHIPE monoliths were prepared by post-functionalization of monolithic surface with 6-aminocaproic acid (ACA) via ring opening reaction of epoxy groups in glycidyl methacrylate (GMA)-based polyHIPEs, and then applied to the preconcentration and determination of trace MG and LMG in environmental water samples by combing with high-performance liquid chromatography (HPLC). Taking MG and LMG as targets, main factors affecting SPE performance of the polyHIPE monoliths were investigated. Under the optimized conditions, the ACA-functionalized polyHIPE monoliths could effectively preconcentrate MG and LMG from 150 mL of water samples, and the recoveries of MG and LMG at three spiked levels were ranged from 84.8 to 97.4% with the relative standard deviations (RSDs) lower than 6%. The proposed method exhibited good linearity in the range of 2–200 ng mL –1 , with low limits of detection of 17.0 and 8.7 pg mL –1 for MG and LMG, respectively. In addition, the prepared ACA-modified polyHIPE monolith showed good durability and stability, and it could be reused for 200 cycles without obvious losing the extraction performance. Graphic abstract
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CHEMOSPHERE
·
2019
·
IF 5.108
Fuyou Du,Lingshun Sun,Zhujun Huang,Zhengyi Chen,Zhigang Xu,Guihua Ruan,Chenxi Zhao
Electrospun reduced graphene oxide/TiO 2 /poly(acrylonitrile- co -maleic acid) composite nanofibers (E-spun RGO/TiO 2 /PANCMA NFs) were fabricated using electrospinning of the dispersive solution of PANCMA, GO and TiO 2 followed by post-chemical reduction. The obtained composite nanofibers were compressed in a dialyzer and then used to absorb and degrade malachite green (MG) and leucomalachite green (LMG) from aqueous solution. Compared to the E-spun TiO 2 /PANCMA and GO/TiO 2 /PANCMA NFs, the E-spun RGO/TiO 2 /PANCMA NFs exhibited higher adsorption capacity and photocatalytic degradation ability. Under optimized conditions, 90.6% of MG and 93.7% of LMG from 50 mL aqueous sample solution were adsorbed on the RGO/TiO 2 /PANMA NFs (3.0 mg fibers) in 2.0 min, and subsequent the 91.4% and 95.2% of MG and LMG adsorbed on the NFs were degradated in 60 min under UV irradiation, respectively. In addition, the E-spun RGO/TiO 2 /PANMA NFs exhibited good reusability and could be reused in multiple cycles of operations for adsorption and photocatalytic degradation of MG and LMG. This work demonstrated that the electrospun composite nanofibers are promising materials for removal of pollutants from environmental water samples.
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MARINE POLLUTION BULLETIN
·
2019
·
IF 3.782
Ji Yang,Zheng-Zhong Lin,Zhi-Yong Huang
A fluorescence probe was delicately designed for the detection of malachite green (MG) in water and fish samples. Through the electrostatic self-assembly of CdTe QDs on the surface of polystyrene (PS) microspheres, the fluorescence signal was amplified. After grafting molecularly imprinted film, the fluorescence probe of MIP@PS@CdTe was fabricated and applied to the detection of MG based on fluorescence quenching . The linear range of MG detection was 0.01–20 μmol L − 1 , and the detection limit was 4.7 nmol L − 1 (3 σ , n = 9) which was much lower than those of the previous reports. The recoveries of MG in aquaculture water and fish samples ranging from 87.6% to 105.4% illustrated that the detection by MIP@PS@CdTe probe was accurate and reliable.
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Journal of Food Measurement and Characterization
·
2019
·Vol. 14
·期 2
·658-667
·
IF 1.415
Zhao Yan,Song Yuying,Zhang Yuanyi,Fan Yuxia,Lai Keqiang
Malachite green (MG) is a banned fish drug, but is often found in various aquatic food products. In this study, Au nanoparticles (NPs) were incorporated with graphene oxide (GO) to fabricate Au NPs-GO hybrid films as surface enhanced Raman scattering (SERS) substrate for MG detection. The enhancement effect of the hybrid substrate for SERS signal of MG as influenced by GO concentration, size of Au NPs and pretreatment method for quartz sheets were evaluated. The optimal Au NPs-GO substrate was used to detect MG in fish muscle with SERS technology. The lowest detectable concentration for MG standard solution was 0.01 ng/mL, and the concentration of 0.25 ng/g could be detected in the muscle extracts of two different fish. SERS combined with Au NPs-GO hybrid substrate shows great potential for detection of trace amounts of MG in aquatic products.
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危险品化学品经营许可证（带存储）