FOOD CHEMISTRY
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2025
·
IF 9.8
Qianqian Lu,Lingling Guo,Xinxin Xu,Hua Kuang,Liqiang Liu,Chuanlai Xu,Maozhong Sun
The mass production and use of organophosphorus pesticides (OPs) have led to a threat to human health. Therefore, establishing a sensitive, rapid, and high-throughput detection method is of great importance. In this study, computer-aided molecular design was firstly applied to design the specific haptens of phorate (PHO), fenthion (FEN), and profenofos (PRO), and high-performance monoclonal antibodies against PHO, FEN, and PRO were prepared. On this basis, a multiplex fluorescent microsphere immunochromatographic assay (FM-ICA) was developed for simultaneous detection of three OPs in cowpea and orange samples. The quantitative detection ranges of PHO, FEN, and PRO were 9.039–140.129, 6.686–108.613, and 5.215–71.479 μg/kg, respectively, in cowpeas; and 7.120–121.566, 3.592–92.185, and 3.579–60.081 μg/kg, respectively, in oranges. The results of recovery and real sample testing showed that the multiplex FM-ICA was rapid and accurate, making it suitable for the on-site testing of a large number of samples.
研究领域:
分析化学
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环境科学
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食品科学
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DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Feng Gong,Zhiyou Tan,Xiaoyun Shan,Yixia Yang,Songbai Tian,Fuxiang Zhou,Xinghu Ji,Zhike He
The digital immunoassay is a highly sensitive detection technique based on single-molecule counting and is widely used in the ultrasensitive detection of biomarkers. Herein, we developed a fluorescent microsphere-based digital immunoassay (FMDIA) by employing fluorescent microspheres as both the carriers for immunoreaction and fluorescent reports for imaging. In this approach, the target protein in the sample was captured by fluorescent microspheres to form a biotin-labeled sandwich immunocomplex, and then, the fluorescent microspheres containing the target protein molecules were captured by adding streptavidin-coated magnetic beads (SA-MBs). By counting the proportion of fluorescence-positive magnetic beads, the concentration of the target protein can be precisely quantified. As a proof of concept, α fetoprotein (AFP) and human interleukin-6 (IL-6) were used to assess the analytical performance of the proposed FMDIA, and limit of detection (LOD) values of 21 pg/mL (0.30 pM) and 0.19 pg/mL (7.3 fM) were achieved, respectively. The results of AFP detection in serum samples of patients and healthy people were consistent with the reference values given by the hospital. Furthermore, by adding fluorescent microspheres of various colors for encoding, the proposed FMDIA can easily realize the simultaneous detection of multiple proteins without the need to introduce multiple modified magnetic beads. This multiplex protein detection strategy, in which the reactions are first carried out on the fluorescent microspheres and then magnetic beads are used to capture the fluorescent reporters containing the target molecules, provides a new idea for digital assays.
研究领域:
生物化学
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医学诊断
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DOI
PubMed
高影响因子
BIOMATERIALS
·
2018
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IF 8.806
Wenjing Zhang,Weiguo Xu,Cong Ning,Mingqiang Li,Guoqing Zhao,Weiqian Jiang,Jianxun Ding,Xuesi Chen
Local anesthetics are a class of drugs, which have wide applications in the treatment of acute and chronic pain. However, their analgesic effects only last for a few hours because of their short half-life, which is insufficient for clinical application, especially for long-term surgery or postoperative analgesia. Herein, an injectable hydrogel/microsphere (GEL/MS) composite co-encapsulating bupivacaine (BUP) and dexmedetomidine (DEX) was developed for effective sustained analgesia. The GEL/MS composite appeared as a three-dimensional porous network microstructure and displayed sustained sequential release of DEX and BUP over several days in vitro , without obvious burst release. In this composite, DEX was released from the GEL matrix preferentially, exhibited long-term vasoconstriction effect and improved the local anesthetic concentration at injection site by preventing BUP from diffusing into the blood circulation. BUP was released subsequently from the MS for blockage of the Na + channel on nerve cell membranes and provided long-term analgesia. In vivo analgesic effect demonstrated that DEX significantly prolonged the effect of analgesia when synergistically administered with BUP in the GEL/MS composite. Moreover, the GEL/MS composite exhibited good biodegradability and biocompatibility in histological analyses. Taken together, the administration of BUP and DEX in the GEL/MS composite achieved a synergistic effect in prolonging analgesia without causing toxicity, and thus represented a potential strategy for long-acting analgesia therapy.
研究领域:
药学
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药物递送系统
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疼痛管理
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry C
·
2019
·Vol. 7
·期 27
·8423-8431
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IF 6.641
Yan Zheng,Qi Jin,Wanyi Chen,Yi Sun,Zhaoqun Wang
In the past decade, stretchable strain sensors have received widespread attention due to their enormous application potential, but it is still a challenge to obtain sensing materials with both high sensitivity and a wide operating range. To this end, we designed a binary structure composed of a polystyrene/graphene conductive microsphere array and graphene nanosheets mingled freely with the microsphere array. Based on this structure, it was found that the properties of the material can be effectively adjusted by controlling the particle size of the polystyrene microspheres and the graphene content. As expected, the bridging effect of this unique binary structure allows the sensor to exhibit high sensitivity (gauge factor = 481), a wide sensing range (maximum sensing strain = 104.5%) and good linearity (R2 ≥ 95%). And the structure has good stability, even after 3000 cycles there is no significant change in the sensing performance. In addition, by introducing one-dimensional oxidized carbon nanotubes and silver nanowires into the sensor to further enhance the bridging effect, the sensitivity and operating range of the sensor are improved again (gauge factor = 1171, maximum sensing strain = 134.4%). The sensor can be used for practical applications such as human motion detection and object mass detection.
研究领域:
材料科学
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传感器技术
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纳米技术
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产品关联
DOI
高影响因子
CERAMICS INTERNATIONAL
·
2019
·
IF 3.45
Wei Hong,Fangwei Guo,Li Hu,Xin Wang,Chen Xing,Yu Tan,Xiaofeng Zhao,Ping Xiao
A hierarchically porous bioactive glass-ceramic microsphere with enhanced bioactivity and good biocompatibility was fabricated via electro spraying technique assisted with non-solvent induced phase inversion. The electro sprayed bioactive glass (ESBG) microspheres were 20 ± 5 μm in diameter, and composed of four characteristic pores: <200 nm, 200–500 nm, 500–1000 nm and >1000 nm. The influences of sintering temperatures (600 °C-1000 °C) on the microstructure, crystalline phase and the related in vitro bioactivity of the samples were investigated. All the ESBG microspheres possessed bioactivity, and the earliest apatite deposition time (3 h) occurred in the samples sintered at 1000 °C owing to the highly porous structure and the formation of wollastonite. The in vitro cytotoxicity was evaluated via CCK-8 assay using MC3T3-E1 cells, revealing the good biocompatibility as well. These results suggest that the ESBG microsphere is a promising material for bone tissue engineering.
研究领域:
生物材料学
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组织工程学
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医学材料
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DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2019
·
IF 9.518
Gang-gang Zhang,Shao-lan Xu,Yong-hua Xiong,Hong Duan,Wen-yao Chen,Xiang-min Li,Mei-fang Yuan,Wei-hua Lai
Fluorescent microsphere (FM) is widely used as probe in immunochromatographic assay (ICA). However, the performance of conventional FM is limited because of the aggregation-caused quenching effect. Herein, we compared a kind of conventional FM (DMFFM, loading DMF) with novel aggregation-induced emission FM (AIEFM, loading TCBPE). The fluorescence intensity of DMFFM initially increased and then decreased as the concentrations of the loading DMF increased. The fluorescence intensity of AIEFM increased as the concentrations of the loading TCBPE increased and retained a high value. AIEFM was compared with two commercial FMs purchased from Ocean (OFM) and Merk (MFM). The maximum fluorescence intensity and relative quantum yield of AIEFM was approximately 5 and 4.5 times higher than those of two commercial FMs. We used the novel AIEFM as a probe to improve the sensitivity of ICA. When Escherichia coli O157:H7 was detected as the target, the limit of detection of ICA based on AIEFM, OFM and MFM were 3.98 × 10 3 CFU/mL, 4.48 × 10 4 and 2.78 × 10 4 CFU/mL, respectively. The ICA of AIEFM had 11 and 7 times improvement in sensitivity compared with that of OFM and MFM. Our results could be used as a basis for novel probes in practical ICA applications.
研究领域:
生物化学
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分析化学
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医学检测
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产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
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2012
·
IF 4.98
Long Lin,Shang-Ru Zhai,Zuo-Yi Xiao,Na Liu,Yu Song,Bin Zhai,Qing-Da An
A simple method to the preparation of SiO 2 microspheres from rice husks (RH) was developed. The rice husks were extracted with 2 M NaOH solution to obtain a extract contained silica and lignin, and silica spheres were obtained after addition of distilled water, ethanol, 1 M H 2 SO 4 and PEG (W silica :W PEG = 2:1) to the extract in a controllable sol–gel process and calcination at 550 °C. The optimum synthesis conditions were, adjustment of the extract to pH 3, a volume ratio of 1:1:2 of ethanol, water, and extract, respectively and a silica to polyethylene glycol weight ratio of 2:1. The SiO 2 microspheres produced under these conditions exhibited a relatively uniform particle size distribution around 500 nm as determined by LPSA, which indicated a cooperative function of added PEG and extracted lignin towards the formation of SiO 2 microspheres.
研究领域:
材料科学
·
化学工程
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产品关联
DOI
PubMed
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2017
·
IF 4.164
Jianbing Wu,Xusheng Xie,Zhaozhu Zheng,Gang Li,Xiaoqin Wang,Yansong Wang
The effects of changing solution pH in the range of 3.6–10.0 during a one-step silk microsphere preparation process, by mixing silk and polyethylene glycol (PEG), was assessed. The microspheres prepared at low pH (3.6) showed a more homogeneous size (1–3 μm) and less porous texture than those prepared at neutral pH. High pH (10.0) inhibited microsphere formation, yielding small and inhomogeneous microspheres. Compared to neutral pH, low pH also increased the content of silk crystalline β-sheet structure from approx. 30% to above 40%. As a result, the microspheres produced at low pH were more thermally stable as well as resistant to chemical (8 M urea) and enzymatic (protease XIV) degradation when compared to microspheres prepared at neutral pH. Doxorubicin hydrochloride (DOX) and curcumin (CUR) were successfully loaded in silk microspheres via control of solution pH. The loading efficiency of DOX was approx. 95% at pH 7.0 and approx. 60% for CUR at pH 3.6, attributed to charge-charge interactions and hydrophobic interactions between the silk and drug molecules, respectively. When PBS, pH 7.4, was used as a medium for release studies, the pH 3.6 microspheres released both drugs more slowly than the pH 7.0 microspheres, likely due to the high content of crystalline β-sheet structure that enhanced drug-silk interactions as well as restricted drug molecule diffusion.
研究领域:
生物材料
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药剂学
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产品关联
DOI
PubMed
高影响因子
International Journal of Nanomedicine
·
2018
·
IF 4.37
Wei Luo,Zhen Geng,Zhaoyang Li,Shuilin Wu,Zhenduo Cui,Shengli Zhu,Yanqin Liang,Xianjin Yang
Background Calcium sulphate cement (CSC) is widely used as an osteoconductive biomaterial in bone repair and regeneration.Purpose In this study, porous TiO2 microspheres were added to CSC to achieve a controlled and sustained drug (gentamicin) release.Methods Scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray powder diffraction (XRD), and Brunauer-Emmett-Teller (BET) surface area analysis were conducted to analyse the morphology, phase composition, and surface area of the TiO2 micro-spheres and composite cements. In addition, the injection time, compressive strength, degradation behaviour, and antibacterial ability of the composite cements were examined during in vitro degradation. Gentamicin release profile was recorded using an ultraviolet spectrophotometer.Results The results revealed the excellent drug loading ability of the TiO2 microspheres. The addition of TiO2 microspheres improved the injectability and compressive strength of the composite cements, the maximum value of which was achieved at a TiO2 loading of 5 wt.%. When immersed in simulated body fluid (SBF), the composite cements doped with TiO2 microspheres were observed to release gentamicin in a stable and sustained manner, especially in the latter stages of in vitro degradation. During degradation, CSC doped with TiO2 microspheres exhibited a typical apatite-like behaviour. Further, antibacterial analysis showed that CSC doped with TiO2 microspheres exhibited long-term antibiotic activity.Conclusion Thus, as an effective sustained-release formulation material, TiO2 microspheres show a great potential for application in bone cements.
研究领域:
生物材料学
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药剂学
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骨科医学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2013
·Vol. 25
·期 2
·754-759
·
IF 1.486
Liu Lu,Huang Miaoliang,Lan Zhang,Wu Jihuai,Shang Guanglu,Liu Guijing,Lin Jianming
Mn-doped CdS quantum dot sensitized solar cells based on SnO 2 microsphere photoelectrodes are prepared with successive ionic layer adsorption and reaction method. It is found that with Mn-doped CdS quantum dot sensitizers, the photovoltaic performance of the cells based on SnO 2 microsphere photoelectrodes can obviously be enhanced. The reasons are owing to the improved light absorption and the expanded light absorption edge by doping Mn in CdS quantum dots. The electrochemical impedance spectroscopy analysis found that the cells with Mn-doped CdS quantum dot sensitized SnO 2 microsphere photoelectrodes can efficiently suppress dark reaction, owing to the increased related resistance. Moreover, it is also found that the Mn-doped CdS quantum dot sensitized SnO 2 microsphere photoelectrode can increase the electron diffusion lifetime in the cell. The power conversion efficiency of the cell with 4 wt% Mn-doped CdS quantum dot sensitizers can attain to 2.80 %, with 53 % enhancement compared with that of the CdS quantum dot sensitized cell (1.83 %).
研究领域:
材料科学
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光伏技术
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纳米技术
·
电化学
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产品关联
DOI
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2017
·Vol. 25
·期 4
·3922-3932
·
IF 2.741
Yi Xiaofeng,Yang Meixia,Mo Liuda,Xu Wenkai,Wang Shuai,He Jiarui,Gu Junjie,Ou Minrui,Xu Xiaoping
Copper in drinking water causes a significant environmental problem. Composite material based on alginate hydrogel has been gaining attention in the field of Cu(II) adsorption. However, alginate-based hydrogel exhibits poor mechanical property and relative low adsorption capacity which limit their application. The present study is devoted to the modification of chitosan/calcium alginate/Fe 3 O 4 (CAF) hydrogel microsphere by NaOH solution for enhancement of Cu(II) adsorption. Results reveal that modification of CAF via NaOH solution significantly improves the mechanical strength and Cu 2+ adsorption capacity of pristine materials. FTIR and XRD analysis confirms that CAF and newly prepared materials (NACAF) are successfully prepared. SEM and EDX are employed to analyze the surface morphology and elemental composition, respectively, both before and after their loading with Cu 2+ . XPS study demonstrates adsorption mechanism is based on chelation and ion-exchange. Compressive stress-strain curves demonstrate NACAF has better mechanical performance than CAF. The adsorption kinetics of the two adsorbents follow a pseudo-second-order model. The equilibrium data were best described by Langmuir isotherm model, and the estimated maximum equilibrium sorption capacity, q
m ,is 261.31 mg/g for the NACAF, which is larger than that of CAF (145.39 mg/g). Hence, NACAF shows excellent mechanical strength and high sorption capacity for Cu 2+ . It has great potential for Cu(II) removal in aqueous solutions.
研究领域:
环境科学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
Molecular Catalysis
·
2021
·
IF 5.062
Yu Guan,Yinhe Liu,Qiang Lv,Bo Wang
The selective catalytic reduction of nitrogen oxides (NO x ) by carbon monoxide (CO-SCR) is a promising way to deal with exhaust gasses. And Effective redox cycle and NO dissociation ability are crucial factors for catalytic reduction of NO x . Herein, Fe decorated CeO 2 microsphere catalysts with surface oxygen defect were synthesized by solvothermal and calcination method. Among all catalysts, 0.5Fe-CeO 2 sample showed excellent catalytic performance with efficiency near 96% for NO reduction under the condition of 550 ppmv NO, 1200 ppmv CO, and 40,000 h − 1 of GHSV. It was found that Fe doping could efficiently regulate Ce 4+ /Ce 3+ redox couple and oxygen vacancy for catalytic activity. XRD, Raman, XPS, EPR and H 2 -TPR etc. were employed to explore the physicochemical properties of samples. The results showed that Fe had been incorporated into CeO 2 by XRD, and the results of Raman spectrum and XPS indicated that Ce 4+ /Ce 3+ redox couple existed in 0.5Fe-CeO 2 catalyst. Furthermore, the H 2 -TPR spectrum displayed the formation of oxygen vacancy in the samples. N 2 adsorption-desorption isotherms indicated that the doping of Fe could enlarge the specific surface area, pore volume and pore dimeter of the samples, and thus greatly enhanced the NO adsorption and further NO catalytic reduction on catalysts. In addition, other characterization of physicochemical properties and density functional theory (DFT) calculation were performed to reveal the mechanism of NO + CO reaction. As a result, a mechanism was proposed to elucidate highly efficient NO reduction over the catalysts of Fe decorated CeO 2 microspheres. Finally, the synergistic effects of redox center and oxygen vacancy on catalytic activity enhancement were revealed. This work provides a promising way to the design of efficient catalysts for NO reduction.
研究领域:
催化化学
·
环境科学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）