APPLIED SURFACE SCIENCE
·
2021
·
IF 6.707
Huimin Sun,Xiuting Li,Ziyang Hu,Chenjie Gu,Dong Chen,Jun Wang,Bin Li,Tao Jiang,Xingfei Zhou
As a typical membrane matrix with attractive flexibility, paper has exhibited great feasibility to serve as surface-enhanced Raman scattering (SERS) substrate in the environmental monitoring and surveillance. In this research, we have designed and fabricated a distinctive hydrophilic-hydrophobic SERS substrate utilizing several ordinary papers loaded with Ag nanowires (Ag NWs). Remarkable SERS activities with an enhancement factor (EF) up to 2.63 × 10 6 was successfully achieved in the assembly Ag NWs with the optimal amount of nanotips and overlaps. The hydrophilic-hydrophobic strategy could contribute to anchoring microdroplets and thus enrich analytes to improve the homogeneity of substrate with a low relative standard deviation of 10.6%. Furthermore, the sophisticated SERS substrate was also applied in the in-situ detection of trace furazolidone on fish under the interference of malachite green and the reliable recovery rates were demonstrated as well.
研究领域:
环境科学
·
分析化学
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产品关联
DOI
高影响因子
OPTICS EXPRESS
·
2023
·Vol. 31
·期 16
·26474-26495
·
IF 3.8
Linlin Xu,Ming Chen,Qingqiang Cui,Chang Wang,Mnegya Zhang,Linqi Zheng,Shuang Li,Hua Zhang,Guangda Liang
Exploring multifunctional surface-enhanced Raman scattering (SERS) substrates with high sensitivity, broadband response property and reliable practicability should be required for ultrasensitive molecular detection in complex environments, which is heavily dependent on the photo-induced charge transfer (PICT) efficiency realized on the desirable nano-architectures. Herein, we introduce ultra-clean ternary Au/Ag/AgCl nanoclusters (NCs) with broadband resonance crossing the visible light to near-infrared region created by one step laser irradiation of mixed metal ion solution. Interestingly, the surface defects and interaction among these unique cluster-like ternary nanostructures would be further enhanced by thermal annealing treatment at 300°C, providing higher broadband SERS activities than the reference ternary nanoparticles under 457, 532, 633, 785, and 1064 nm wavelengths excitation. More importantly, the further promoted SERS activities of the resultant Au/Ag/AgCl NCs with achievable ∼5-fold enhancement than the initial one can be conventionally realized by simplistically declining the temperature from normal 20°C to cryogenic condition at about -196°C, due to the lower temperature-suppressed non-radiative recombination of lattice thermal phonons and photogenerated electrons. The cryogenic temperature-boosted SERS of the resultant Au/Ag/AgCl NCs enables the limit of detection (LOD) of folic acid (FA) biomolecules to be achieved as low as 10−12 M, which is obviously better than that of 10−9 M at room temperature condition. Overall, the smart Au/Ag/AgCl NCs-based broadband SERS sensor provides a new avenue for ultrasensitive biomolecular monitoring at cryogenic condition.
研究领域:
材料科学
·
分析化学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Rui Tan,Ziji OuBu,Qun Zhou,Juan Mu,Chenxi Yi,Xiaojun Luo
The presence of dexamethasone (DEX) residues and the illegal addition of DEX to food have garnered widespread public concern over recent decades. Based on the bicycled hairpin assembly (BHA) mechanism and the catalytic oxidase effect of urchin-shaped Au–Ag alloy loaded with Pt nanoparticles (Au@Ag@Pt), an accurate and ultrasensitive surface-enhanced Raman scattering (SERS)/colorimetric dual-mode method for DEX detection is pioneered. When DEX is introduced into the sensor system, the BHA cycle begins. The Fe3O4 nanosphere loaded with Au nanoparticles (Fe3O4@Au) surface facilitates the anchoring of a large amount of Au@Ag@Pt, resulting in synergistic signal amplification and facile magnetic separation from the complex system. Finally, the collected product effectively catalyzes the conversion of 3,3′,5,5′-tetramethylbenzidine (TMB) to the oxidized product, producing a strong SERS response, an ultraviolet–visible absorption signal, and color deepening. Our dual-mode platform achieves ultrasensitive detection of DEX, achieving a detection limit (LOD) of 6.61 × 10–17 M within the broad range of 10–16 to 10–9 M for SERS and a LOD of 8.81 × 10–13 and 6.98 × 10–13 M within the range of 10–12–10–5 M for the colorimetric method. Additionally, this platform shows high selectivity and satisfactory recovery rates for DEX in milk and pork samples, with recoveries of 92.3%–106.5% (RSDs <8.35%) for SERS and 95.2%–107.6% (RSDs <8.81%) for colorimetric analysis. This study demonstrates that by combining the BHA amplification process with multifunctional Au@Ag@Pt and Fe3O4@Au substrates, the DEX content in actual food samples can be accurately determined using a dual-mode approach that combines the high sensitivity of SERS with the simplicity of colorimetric analysis.
研究领域:
分析化学
·
食品安全
·
纳米生物技术
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2025
·
IF 2.6
Changqing Du,Anran Li,Jinye Qiu,Jiarui Sun,Xiaokun Wang,Wei Xu,Maofeng Zhang,Dapeng Wang
Quantitative mRNA expression levels are essential for differential and clinical diagnoses. In this work, we present an apurinic/apyrimidinic endonuclease 1 (APE1)-assisted SERS sensing platform for rapid and ultrasensitive detection of TK1 mRNA. This platform utilizes a hairpin probe that contains a TK1 mRNA capture sequence along with an apurinic/apyrimidinic (AP) sites, which is specifically recognized and cleaved by APE1. Upon introduction of the target mRNA, hybridization occurs between the mRNA and the AP probe (HAP) within the hairpin structure, forming a doublestranded complex. APE1 then cleaves the HAP at the specific AP sites within the double-stranded complex, leading to a measurable change in the SERS intensity of the FAM-labeled HAP. Based on this principle, the developed SERS sensing platform demonstrates a highly linear response for TK1 mRNA detection across a wider range from 1 fM to 10 nM, with a detection limit (LOD) of 0.2 fM. Moreover, the SERS sensing platform shows great potential for the precise and quantitative detection of TK1 mRNA in human serum, which could be instrumental for mRNA-related research and the early clinical diagnosis of diseases.
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Honglian Wu,Ruolin Zhang,Huang Lai,Luogen Lai,Yi Liu,Yadi Fan,Yu Zhang,Jingyu Shi,Mo Yang
Endotoxins or lipopolysaccharides (LPS) are potent inflammatory agents that pose serious health risks even at low concentrations, thus requiring sensitive and reliable detection methods. Current methods for endotoxin detection, primarily the Limulus Amebocyte Lysate (LAL) assay and Enzyme-linked Immunosorbent Assays (ELISA) are limited by complex procedures, prolonged assay time, and susceptibility to interference. To address these challenges, we developed a single-reporter probe-based dual-mode biosensor integrating MXene-Au nanosheets with the CRISPR/Cas12a system for endotoxin detection. The sensing platform was constructed by conjugating ssDNA-Cy5 reporters onto MXene-Au nanosheets. MXene-Au nanosheets served dual functions, including efficient quenching of fluorescence and strong enhancement of SERS signals. In the presence of target endotoxins, CRISPR/Cas12a remains inactive, leaving the ssDNA-Cy5 reporters intact on the MXene-Au surface. The close proximity of Cy5 to the MXene-Au surface results in quenched fluorescence (“FL off”) but enhanced SERS signals (“SERS on”). Conversely, in the absence of endotoxins, activated CRISPR/Cas12a cleaves the ssDNA-Cy5, releasing Cy5 from the surface, thus leading to fluorescence recovery (“FL on”) but diminished SERS signals (“SERS off”). By measuring the changes in SERS and FL signals and analysing the ratiometric I SERS /I FL signals, the biosensor achieved a lower limit of detection (LOD) of 15.9 pg/mL within 30 minutes compared to single detection modes. Validation studies in complex matrices, including spiked water and milk samples, showed performance comparable to commercial endotoxin detection kits. Overall, our CRISPR-Cas12a-mediated fluorescent-SERS dual-mode biosensor not only improves detection sensitivity but also provides built-in quality control, improving the reliability of results through cross-validation.
研究领域:
生物医学工程
·
分子诊断学
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产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2019
·
IF 2.931
Xingying Zhang,Ben Liu,Chenglong Hu,Shaoyun Chen,Xueqing Liu,Jiyan Liu,Fang Chen,Jian Chen,Fangyan Xie
Silver nanowires (i.e., AgNWs) can act as effective surface-enhanced Raman spectroscopy (i.e., SERS) substrates to detect small molecules. However, a lot of prepared AgNWs were often wrapped by polyvinylpyrrolidone (i.e., PVP) thin film to form an insulating layer to produce ill-defined AgNWs-PVP-AgNWs interface, limiting the plasmonic coupling among the stacked AgNWs. Herein, we reported a facile method in removal of PVP ligands from AgNWs for high performance and reusable SERS substrate. Sodium borohydride (NaBH 4 ) was used to completely remove the PVP ligands from the surface of AgNWs and produce a clean AgNWs-AgNWs interface that effectively enhances the localized surface plasmon resonance (i.e., LSPR) was produced, greatly improving the SERS activity of the AgNWs thin film. The SERS detection of rhodamine 6G (i.e., R6G) used with PVP AgNWs and without PVP AgNWs is 1.0 × 10 -9 and 1.0 × 10 -15 M, and the average enhancement factor ( EF ) is about 0.86 × 10 4 and 9.35 × 10 4 , respectively. Moreover, the recyclable behavior of the AgNWs with several analyte molecules is much more interesting than that of the PVP@AgNWs. The SERS detection of AgNWs for R6G, the 3-mercaptopropionic acid (i.e., 3-MPA) and melamine with good recyclability in nanomolar and millimolar concentration can be easily detected.
研究领域:
材料科学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Haonan Wang,Chuyi Wang,Jiali Huang,Yunzhen Liu,Yang Wu,Ruiyun You,Jian-Han Zhang,Yudong Lu,Huiying Shen
As a widely used industrial additive of plastic products, phthalate ester (PAE) plasticizers can easily migrate into food, threatening human health. In this work, we proposed a rapid, precise, and reliable method to detect PAE plasticizers in edible oils by using surface-enhanced Raman spectroscopy (SERS) technology. A two-dimensional (2D) silver plate synergizing with a nanosilver sol was prepared as a substrate for SERS to detect potassium hydrogen phthalate (PHP), a hydrolysate of a PAE plasticizer. Detection conditions, such as pH values, drying times, and hydrolysate interference, were optimized. The working curve was well fitted with a linear parameter R 2 of 0.9994, and the minimum detection limit was evaluated as 10 −9 mol/L. Furthermore, the detection accuracy was supported by five edible oil samples. Therefore, using SERS technology to detect PHP is expected to provide an avenue for PAE plasticizer detection in oils and fats, and it features promising potential applications in food safety.
研究领域:
食品安全
·
分析化学
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产品关联
DOI
PubMed
高影响因子
FOOD AND AGRICULTURAL IMMUNOLOGY
·
2022
·
IF 3.268
Peizhen Li,Xiuhua Xia,Jiannan Chen,Hang Yu,Yunfei Xie,Yahui Guo,Weirong Yao,He Qian,Yuliang Cheng
Gold-coated silver core–shell nanoparticles (Ag@Au NPs) have been widely used for SERS analysis due to plasmon resonance performances. However, it is a great challenge to fabricate Ag@Au core–shell structures with specified morphology. In this paper, the size and shape of Ag NPs (core) were precisely controlled by a seed-mediated approach, and then the gold shell was completely coated on under the strong reduction of ascorbic acid, resulting in Ag@Au NPs with highly regulated morphology. The obtained Ag@Au NPs achieved a similar or even better SERS enhancement factor (EF) (up to 5.7 × 107) compared to Ag NPs, but significantly higher stability. For the rapid SERS detection of nitrofurantoin metabolite AHD, a limit of detection (LOD) as low as 1 × 10−10 M and a wide linear range from 1 × 10−4 to 1 × 10−8 M were obtained. Furthermore, recoveries for crayfish samples were from 90.5% to 108%, indicating a good performance of the presented method in real sample detection.
研究领域:
分析化学
·
食品安全
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
Analytical Methods
·
2025
·
IF 2.6
Xiulei Xiong,Haihua Shen,Songnan Liu,Fei Rong,Jian Dong
To detect H2O2 and redox potential of an acute wound simultaneously, a SERS-active microneedle was fabricated by integrating H2O2 and redox potential SERS probes in two grooves of an acupuncture needle, respectively. When the SERS-active microneedle was inserted into tissues, an acute wound formed and the two SERS probes were brought into the wound to sense H2O2 and redox potential of the wound. The feasibility of the SERS-active microneedle was evaluated ex vivo and in vivo. Both sugar deficiency and sugar excess induce the increases of H2O2 and redox potential in most tissues. Short-term physiological disturbances eventually returned to normal levels in healthy organisms, and abnormal sugar intakes disrupt the normal physiological environment and induce stress response in all tissues. The SERS-active microneedle for detection of H2O2 and redox potential would become a powerful tool, used for collecting physiological signals in different tissues to elucidate the mechanisms of wound healing and redox-related diseases.
研究领域:
生物医学工程
·
分析化学
·
病理学
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产品关联
DOI
PubMed
高影响因子
VIBRATIONAL SPECTROSCOPY
·
2025
·
IF 3.1
Xubo Wang,Hongjun Yin,Jingwen Zhu,Yong Huang,Zhenguang Wang,Xinmei Zheng,Xiaowei Cao,Wei Wei
MicroRNA (miRNA) is a potential biomarker for tumor diagnosis. Therefore, it is important to investigate a method for accurate analysis of miRNA. In this study, we developed a novel and cost-effective surface-enhanced Raman scattering (SERS)-lateral flow assay(LFA) dual detection line test strip for quantitatively detecting miR-19a-3p and miR-4491, respectively. These two miRNAs are closely associated with the development of gastric cancer (GC) and their significant overexpression in patients gives them great potential as innovative tumor markers for GC detection. During the detection process, miR-19a-3p and miR-4491 would compete for binding to the corresponding SERS tags. The presence of concentrations of miR-19a-3p and miR-4491 in the sample solution reduces the binding of the SERS tags to the ssDNAs, thereby reducing the SERS intensity. The test strips were designed to be highly specific and reproducible, and the entire assay was completed in 15 minutes. The test strips we designed were simpler, more sensitive, and more efficient than other detection methods. The detection limit in serum were 21.14 fM for miR-19a-3p and 10.34 fM for miR-4491. In addition, the results of SERS-based LFA test strip and quantitative real-time polymerase chain reaction (qRT-PCR) were consistent for serum testing of healthy individuals, patients with mild intraepithelial neoplasia (IE), patients with severe IE, and patients with gastric cancer. Therefore, SERS-based LFA test strip has great potential for the clinical diagnosis of gastric cancer.
研究领域:
生物医学
·
分子诊断
·
癌症研究
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产品关联
DOI
高影响因子
TALANTA
·
2017
·
IF 4.162
Dan Sun,Guohua Qi,Fanghao Cao,Weiqing Xu,Qidan Chen,Shuping Xu
A highly sensitive and recyclable surface-enhanced Raman scattering (SERS) chip sensor is designed for the determination of silver ions . It is based on the specific reversible binding between silver ions and glucose oxidase (GOD). The sensing chip is made by layer-by-layer assembling GOD on a metal nanoparticle-assembled, SERS-active chip with a positive charged polyelectrolyte as a linker. Iso-alloxazine, a chromophore in flavin adenine dinucleotide (FAD) in GOD, coordinates with silver ions, which causes significant variation in SERS spectra of GOD. The reaction shows high sensitivity and selectivity for silver ions with the detection limit of 1.0×10 −10 M. Most significantly, the bonded Ag(I) in GOD can be reduced to Ag(0) by sodium borohydride and the GOD structure can be recovered then. Thus, the chip sensor is recyclable. Merits of using GOD as a novel SERS probe toward Ag + are embodied in not only a simplified sensor structure based on its dual role of Ag + accepter and SERS signal reporter, but also a nontoxic label to biological systems, indicating that it is promising for tracing Ag + in vivo.
研究领域:
分析化学
·
环境科学
·
材料科学
·
生物技术
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产品关联
DOI
PubMed
高影响因子
Nanoscale
·
2014
·Vol. 6
·期 14
·8155-8161
·
IF 6.739
Dan Zhu,Zhuyuan Wang,Shenfei Zong,Hui Chen,Xin Wu,Yuwei Pei,Peng Chen,Xueqin Ma,Yiping Cui
A liposome–Ag nanohybrid has been demonstrated as a SERS traceable intracellular drug nanocarrier. Liposomes have been introduced for their special qualities in drug delivery systems. In essence, 4-aminothiophenol (4ATP) tagged Ag nanoparticles (Ag@4ATP) were adsorbed onto the surfaces of liposomes via electrostatic interactions, in which 4ATP was used as a SERS reporter. In such a nanohybrid, the locations of the carrier can be tracked by SERS signals while those of the drugs can be monitored through their fluorescence, allowing the simultaneous investigation of the intracellular distribution of both the carriers and the drugs. Our experimental results suggest that the reported liposomal system has substantial potential for intracellular drug delivery.
研究领域:
纳米技术
·
药学
·
生物医学工程
·
细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
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