ACS Applied Materials & Interfaces
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2022
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IF 10.383
Peng Ning,Yingna Chen,Qianwen Bai,Chang Xu,Cuijun Deng,Qian Cheng,Yu Cheng
Stem cell therapy has shown great potential in treating a wide range of diseases including cancer. The real-time tracking of stem cells with high spatiotemporal resolution and stable imaging signals remains the bottleneck to evaluate and monitor therapeutic outcomes once transplanted into patients. Here, we developed a photosensitive mesenchymal stem cell (MSC) loaded with mesoporous silica-coated gold nanostars (MGNSs) integrated with indocyanine green for spatiotemporal tracking and imaging-guided photothermal therapy (PTT) in treating breast cancers. The MGNS served as a stable imaging probe with multifunctional properties for photoacoustic imaging (PAI), fluorescence imaging, and PT imaging. Owing to the excellent PT stability of MGNSs, long-term three-dimensional (3D) PAI was achieved to monitor stem cells in real time at the tumor site, while the tumor structure was imaged using 3D B-mode ultrasound imaging. PAI revealed that the photosensitive stem cells reached the widest distribution area at the tumor site post 24 h of intratumoral injection, which was further confirmed via two-dimensional (2D) PT and fluorescence imaging. With this optimal cell distribution window, in vivo studies showed that the photosensitive stem cells via both intratumoral and intravenous injections successfully inhibited breast cancer cell growth and decreased the tumor recurrence rate post PTT. Our results support that this photo-integrated platform with stable optical properties is promising to achieve real-time tracking and measure the cell distribution quantitatively with high spatiotemporal resolution for stem cell therapy.
研究领域:
癌症治疗
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纳米技术
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医学影像
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干细胞研究
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DOI
PubMed
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
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2018
·Vol. 16
·期 1
·1-15
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IF 5.294
Li Yuanyuan,Shi Sanjun,Ming Yue,Wang Linli,Li Chenwen,Luo Minghe,Li Ziwei,Li Bin,Chen Jianhong
Background Cancer stem cells (CSCs) are highly proliferative and tumorigenic, which contributes to chemotherapy resistance and tumor occurrence. CSCs specific therapy may achieve excellent therapeutic effects, especially to the drug-resistant tumors. Results In this study, we developed a kind of targeting nanoparticle system based on cationic albumin functionalized with hyaluronic acid (HA) to target the CD44 overexpressed CSCs. All-trans-retinoic acid (ATRA) was encapsulated in the nanoparticles with ultrahigh encapsulation efficiency (EE%) of 93% and loading content of 8.37%. TEM analysis showed the nanoparticles were spherical, uniform-sized and surrounded by a coating layer consists of HA. Four weeks of continuously measurements of size, PDI and EE% revealed the high stability of nanoparticles. Thanks to HA conjugation on the surface, the resultant nanoparticles (HA-eNPs) demonstrated high affinity and specific binding to CD44-enriched B16F10 cells. In vivo imaging revealed that HA-eNPs can targeted accumulate in tumor-bearing lung of mouse. The cytotoxicity tests illustrated that ATRA-laden HA-eNPs possessed better killing ability to B16F10 cells than free drug or normal nanoparticles in the same dose, indicating its good targeting property. Moreover, HA-eNPs/ATRA treatment decreased side population of B16F10 cells significantly in vitro. Finally, tumor growth was significantly inhibited by HA-eNPs/ATRA in lung metastasis tumor mice. Conclusions These results demonstrate that the HA functionalized albumin nanoparticles is an efficient system for targeted delivery of antitumor drugs to eliminate the CSCs.
研究领域:
肿瘤学
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纳米技术
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药学
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分子生物学
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PubMed
高影响因子
JOURNAL OF WUHAN UNIVERSITY OF TECHNOLOGY-MATERIALS SCIENCE EDITION
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2018
·Vol. 33
·期 6
·1437-1443
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IF 0.524
Zhou Wei,Fu Wenxing,Zhang Yucang
Banana pseudo-stem was liquefied in the mixture of polyhydric alcohols of polyethylene glycol (PEG400) and glycerol. Hydroxyl value of liquefied products ranged from 294.8 to 370.2 mg KOH/g and \({\bar M_n}\) was about 430. Liquefied products (LBPP) could be used as raw materials for polyurethane by reacting with 4, 4’-diphenylmethane diisocyanate (4, 4’-MDI) and PEG400 to synthesize liquefied product-based polyurethane (LBPP-PU) adhesive. To analyze in depth the creation of urethane linkage among LBPP, PEG400 and 4, 4’-MDI, factors which had effects on the residue content were all investigated. They were characterized by FT-IR and TG. The shear strength of LBPP-PU adhesive was improved when decreasing the percentage of the substitution of PEG400 by LBPP. The adhesive strength was obtained from T-peel of aspen/polyurethane adhesive joints, and the maximum lap shear strength (4.40 MPa) was obtained when 16.70% of LBPP was added to the LBPP & PEG400 system.
研究领域:
材料科学
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生物化学
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DOI
高影响因子
EMBO REPORTS
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2020
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IF 7.497
Gang Du,Yicheng Qiao,Zhangpeng Zhuo,Jiaqi Zhou,Xiaorong Li,Zhiming Liu,Yang Li,Haiyang Chen
The age-associated decline of adult stem cell function is closely related to the decline in tissue function and age-related diseases. However, the underlying mechanisms that ultimately lead to the observed functional decline of stem cells still remain largely unexplored. This study investigated Drosophila midguts and found a continuous downregulation of lipoic acid synthase, which encodes the key enzyme for the endogenous synthesis of alpha-lipoic acid (ALA), upon aging. Importantly, orally administration of ALA significantly reversed the age-associated hyperproliferation of intestinal stem cells (ISCs) and the observed decline of intestinal function, thus extending the lifespan of Drosophila. This study reports that ALA reverses age-associated ISC dysfunction by promoting the activation of the endocytosis–autophagy network, which decreases in aged ISCs. Moreover, this study suggests that ALA may be used as a safe and effective anti-aging compound for the treatment of ISC-dysfunction-related diseases and for the promotion of healthy aging in humans.
研究领域:
细胞生物学
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衰老研究
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干细胞生物学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Chemistry
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2022
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IF 5.545
Ding Jie,Xu Weiqiang,Tan Jing,Liu Zhifang,Huang Guoliang,Wang Shoushan,He Zhiwei
Antigen CD133 is a glycoprotein present on the surface of cancer stem cells (CSCs), which is a key molecule to regulate the fate of stem cells and a functional marker of stem cells. Herein, a novel fluorescence “turn-on” nano-aptamer sensor for quantifying CD133 was designed using hybridization between CD133-targeted aptamers and partially complementary paired RNA (ssRNA), which were modified on the surface of quantum dots (QDs) and gold nanoparticles (AuNPs), respectively. Owing to the hybridization of aptamers and ssRNA, the distance between QDs and AuNPs was shortened, which caused fluorescence resonance energy transfer (FRET) between them, and the florescence of QDs was quenched by AuNPs. When CD133 competitively replaced ssRNA and was bound to aptamers, AuNPs-ssRNA could be released, which led to a recovery of fluorescent signals of QDs. The increase in the relative value of fluorescence intensity was investigated to linearly correlate with the CD133 concentration in the range of 0–1.539 μM, and the detection limit was 6.99 nM. In confocal images of A549 cells, the CD133 aptamer sensor was further proved applicable in lung cancer cell samples with specificity, precision, and accuracy. Compared with complicated methods, this study provided a fresh approach to develop a highly sensitive and selective detection sensor for CSC markers.
研究领域:
生物医学工程
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分子生物学
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纳米技术
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD PROPERTIES
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2022
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IF 3.388
Rui Shi,Bingjie Xiong,Shu He,Can Liu,Jiftah Ben-Asher,Abraham Rami Horowitz,Shu Wang,Xiahong He
Panax notoginseng is highly used in traditional Chinese medicine, and its root is valued for ginsenoside contents. However, evidence suggests that other plant parts such as fruit, leaves, and stem are also potential sources of bioactive compounds. Therefore, this study aimed at providing insight into the differential accumulation of metabolites in fruit, leaf, root, and stem tissues collected from P. notoginseng. A total of 808 metabolites from 11 major metabolite classes were identified. Furthermore, 32 Ginsenosides with six conserved Ginsenosides Rg1, Rf, St-3, R1, Ro, Rc, and five conserved notoginsenosides R1, K, M, E, Rb1, were identified with differential accumulation in fruit, leaf, stem, and root tissues. Several other metabolites known for their potential roles in human health were also identified as differentially accumulated in the tissues. Furthermore, the accumulation pattern of ginsenosides in different tissues is highly suggestive of utilizing fruit, leaf, and stem tissues along with roots for value addition of P. notoginseng.
研究领域:
植物学
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药用植物研究
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代谢组学
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DOI
高影响因子
ACS Applied Materials & Interfaces
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2022
·
IF 10.383
Yujia Wei,Maohua Chen,Menghuan Li,Dong Wang,Kaiyong Cai,Zhong Luo,Yan Hu
Endowing bone regeneration materials with both stem cell recruitment and osteoinduction properties is a key factor in promoting osseointegration of titanium (Ti) implants. In this study, Apt19s-grafted oxidized hyaluronic acid (OHA) was deposited onto a protein-mediated biomineralization hydroxyapatite (HAp) coating of Ti. HAp was achieved by the treatment of lysozyme and tris(2-carboxyethyl) phosphonate mixture and then soaked in calcium ion (Ca2+) solution to obtain functional Ti substrate (Ti/HAp/OHA-Apt). In vitro studies confirmed that Ti/HAp/OHA-Apt could effectively maintain the sustained release of Apt19s from Ti for 7 days. The released Apt19s significantly enhanced the migration of bone marrow mesenchymal stem cells (MSCs), which was reflected by the experiment of transwell assay, wound healing, and zymogram detection. Compared with pure Ti, Ti/HAp/OHA-Apt was able to adjust the adsorption of functional proteins at the Ti-based interface to expose their active sites, which significantly increased the expression of adhesion-associated proteins (vinculin and tensin) in MSCs to promote their adhesion on Ti-based interface. In vitro cell experiments of alkaline phosphatase activity staining, mineralization detection, and expression of osteogenesis-related genes showed that Ti/HAp/OHA-Apt significantly enhanced the osteogenic differentiation ability of MSCs, which may be highly related to the porous structure of hydroxyapatite on Ti interface. In vivo test of Micro-CT, H&E staining, and histochemical staining further confirmed that Ti/HAp/OHA-Apt was able to promote MSC recruitment at the peri-implant interface to form new bone. This work provides a new approach to develop functional Ti-based materials for bone defect repair.
研究领域:
生物材料
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组织工程
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骨科
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DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
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2022
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IF 5.999
Yuhui Wang,Shilong Ma,Xuanyu Liu,Yan Wei,Haofeng Xu,Ziwei Liang,Yinchun Hu,Xiaojie Lian,Di Huang
Hepatocellular carcinoma (HCC) is one of the deadliest tumors in the world with a high rate of recurrence and metastasis. Therefore, the most pressing issue today is the development of new drugs, diagnostic and therapeutic approaches for effective cancer treatment. Cancer stem cells (CSCs) play a pivotal role in tumor recurrence, tumor resistance, and tumor metastasis, which provides a new perspective on the development of liver cancer. In the study, a high-temperature thermal breakdown approach was used to create composite magnetic nanocubes modified by polyethyleneimine (PEI) and hyaluronic acid (HA). The Fe 3 O 4 nanocubes can recognize HCC stem cells via receptor−ligand binding of HA and CD44 (HA receptor). While loading a small molecule LDN193189 inhibited the expression of stemness-related genes OCT4 and Nanog . More crucially, the Fe 3 O 4 nanocubes significantly suppressed HCC cell proliferation and migration by regulating the expression of epithelial-mesenchymal transition (EMT) process markers E-cadherin, Vimentin , and N-cadherin. Dual targeting using magnetic and receptor-mediated targeting improved the uptake of the drug delivery system. Our findings imply that the medication delivery method might be a potential therapeutic strategy for HCC.
研究领域:
癌症生物学
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纳米技术
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药学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
TISSUE & CELL
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2023
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IF 2.6
Yujun Zhao,Xiaotie Wang,Keke Nie
Background Osteoarthritis is a main cause of deformity in aging people. The chondrogenesis of human adipose-derived stem cells (hADSCs) has a positive effect on the cure of osteoarthritis. However, the regulatory mechanism of hADSC chondrogenesis still needs further exploration. This research investigates the role of interferon regulatory factor 1 (IRF1) in the chondrogenesis of hADSCs. Methods hADSCs were purchased and cultured. The interaction between IRF1 and hypoxia inducible lipid droplet associated (HILPDA) was predicted by bioinformatics analysis, and verified through dual-luciferase reporter and chromatin immunoprecipitation assays. The expressions of IRF1 and HILPDA in osteoarthritis cartilage samples were measured through qRT-PCR. After hADSCs were transfected or further induced for chondrogenesis, the chondrogenesis was visualized by Alcian blue staining, and the expressions of IRF1, HILPDA and chondrogenesis-related factors (SOX9, Aggrecan , COL2A1, MMP13, MMP3) were determined through qRT-PCR or Western blot . Results HILPDA bound to IRF1 in hADSCs. IRF1 and HILPDA levels were up-regulated during the chondrogenesis of hADSCs. Overexpressions of IRF1 and HILPDA promoted the chondrogenesis of hADSCs with the up-regulation of SOX9 , Aggrecan and COL2A1 and the down-regulation of MMP13 and MMP3 ; however, IRF1 silencing generated the opposite effects. Besides, HILPDA overexpression reversed the effects of IRF1 silencing on inhibiting chondrogenesis of hADSCs and regulating the expressions of chondrogenesis-related factors. Conclusion IRF1 promotes the chondrogenesis of hADSCs through up-regulating HILPDA level, providing novel biomarkers for treating osteoarthritis.
研究领域:
干细胞生物学
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骨科
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分子生物学
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产品关联
DOI
PubMed
高影响因子
COMPOSITE INTERFACES
·
2023
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IF 2.6
Shi Jiawei,Bai Xuemeng,Zheng Yahui,Che Jianfei,Xiao Yinghong
A novel bone tissue engineering material with a merit of good biocompatibility and excellent mechanical properties was fabricated with a top-down method. In this work, sugarcane was first treated in NaClO2/acetate buffer to remove lignin, i.e. delignification, and the effect of delignification on pore size of sugarcane is explored bby varying the soaking time in the buffer. Results show that 4 h is the optimal delignification time to ensure good mechanical properties of the sugarcane. The subsequent surface modification with alendronate trihydrate has an advantage to facilitate the final deposition of hydroxyapatite (HAp) due to the fact that the introduction of phosphate groups can effectively improve the nucleation efficiency of HAp. We found that the deposited HAp on sugarcane stem templates can form a bioactive hybrid scaffold material with a hierachical pore structure. Fortunately, during the delignification process of the cellulose structure in the sugarcane stem is not destroyed, thus the hierarchical pore structure remains. The final obtained hybrid scaffold presents a three-dimensional anisotropic porous structure and its longitudinal strength is much higher than the radial strength. The anisotropic property can provide a good mechanical support for scaffold applications. Moreover, the CCK-8 test shows that the cell viability is higher than 95%, indicating that the scaffold exhibits no cytotoxicity. Our investigation demonstrates that the presented hybrid scaffold derived from sugarcane stem has a great potential in the application of bone tissue engineering.
研究领域:
生物材料学
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组织工程学
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2023
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IF 15.1
Wenjuan Wang,Di Zhang,Zeyu Jiang,Xinke Zhang,Yue Jiang,Yuxia Luan
Chemotherapy remains the primary treatment for tumors due to its systemic tumor-suppressing ability. However, the efficacy of chemotherapy is limited by drug resistance in cancer stem cells (CSCs), which play a crucial role in tumor progression and metastasis. In this study, we present a novel nanodrug that enhances the effectiveness of chemotherapy by simultaneously targeting CSCs in the tumor tissue and preventing tumor cells from transforming into CSCs. The nanodrug is formed through the self-assembly of doxorubicin (DOX) and the focal adhesion kinase (FAK) inhibitor PF-573228 (PF). Upon accumulation at the tumor site, DOX induces tumor cell death, while PF overcomes CSCs resistance to DOX by suppressing the level of tyrosine 397 phosphorylated FAK (FAK pY397) in CSCs. Furthermore, PF inhibits the acquisition of CSC phenotype in tumor cells by suppressing the process known as epithelial-mesenchymal transition (EMT). Consequently, the designed nanodrug exhibits a robust ability to address tumor progression and metastasis. This study provides a unique strategy to tackle the challenges posed by CSCs in cancer chemotherapy.
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产品关联
DOI
高影响因子
Advanced Healthcare Materials
·
2023
·
IF 10
Lang Bai,Qibin Han,Zeyu Han,Xiaoyu Zhang,Jingwen Zhao,Huitong Ruan,Junliang Wang,Feng Lin,Wenguo Cui,Xing Yang,Yuefeng Hao
Stem cell tissue engineering is a potential treatment for osteoarthritis. However, the number of stem cells that can be delivered, loss of stem cells during injection, and migration ability of stem cells limit applications of traditional stem cell tissue engineering. Herein, kartogenin (KGN)-loaded poly(lactic -co- glycolic acid) (PLGA) porous microspheres is first engineered via emulsification, and then anchored with chitosan through the amidation reaction to develop a new porous microsphere (PLGA-CS@KGN) as a stem cell expansion vector. Following 3D co-culture of the PLGA-CS@KGN carrier with mesenchymal stem cells (MSCs), the delivery system is injected into the capsule cavity in situ. In vivo and in vitro experiments show that PLGA-CS microspheres have a high cell-carrying capacity up to 1 × 10 4 mm −3 and provide effective protection of MSCs to promote their controlled release in the osteoarthritis microenvironment. Simultaneously, KGN loaded inside the microspheres effectively cooperated with PLGA-CS to induce MSCs to differentiate into chondrocytes. Overall, these findings indicate that PLGA-CS@KGN microspheres held high cell-loading ability, adapt to the migration and expansion of cells, and promote MSCs to express markers associated with cartilage repair. Thus, PLGA-CS@KGN can be used as a potential stem cell carrier for enhancing stem cell therapy in osteoarthritis treatment.
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PubMed
高影响因子
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