Frontiers in Microbiology
·
2024
·
IF 4
Wang Ying,Song Lin,Chen Kun,Shi Chao,Peng Chuantao,Yang Qingli,Li Zhaojie
Protocatechualdehyde (PA) is a phenolic acid present in many plants and has many biological activities. Herein, the antagonistic effects and the action mechanism of PA against methicillin-resistant Staphylococcus aureus (MRSA) were studied. The results showed that PA had both significant antibacterial and anti-biofilm activities against MRSA. Additionally, PA had synergy with ampicillin against MRSA. It was elucidated that PA was prominent in destroying cell membranes, increasing cell membrane permeability and intracellular ROS production, thus leading to bacterial cell damage. Transcriptome analysis showed that PA disrupts many physiological pathways, including increasing cell membrane permeability, inhibiting biofilm formation, decreasing resistance to antimicrobial agents, and impairing DNA replication. Finally, the antimicrobial preservation test showed that PA could inhibit the growth of MRSA and prevent the corruption of beef. In summary, PA is an effective natural antibacterial substance and has a good application potential in food preservation, even in tackling antibiotic resistance problems.
研究领域:
微生物学
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药理学
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食品科学
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分子生物学
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DOI
PubMed
高影响因子
RSC Advances
·
2024
·Vol. 14
·期 53
·39779
·
IF 3.9
Hongshuang Qin,Huaying Niu,Yanxiang Guo,Xiaoting Wang,Tao Liu,Chuanqi Zhao
Photodynamic therapy (PDT) has emerged as an effective way to deal with drug-resistant bacterial infections. Especially, blue light (BL) mediated PDT (BL-PDT) presents unique advantages in the treatments of skin infection due to the strong light absorption of superficial skin, weak penetration of BL and little damage to deep tissues. However, the photosensitizers used for BL-PDT are very limited, and the ongoing development of novel BL photosensitizers is indispensable. Porphyrins are good sources for developing efficient photosensitizers. Herein, for developing more effective BL photosensitizers, five porphyrin derivatives that can be excited by BL [5,10,15,20-tetraphenylporphyrin (TPP), 5,10,15,20-tetrakis(4-bromophenyl)porphyrin (TBPP), 5,10,15,20-tetrakis(4-chlorophenyl)porphyrin (TCPP), 5,10,15,20-tetrakis(4-fluorophenyl)porphyrin (TFPP), 5,10,15,20-tetrakis(4-iodophenyl)porphyrin (TIPP)] are subjected to the investigation of PDT against MRSA (methicillin resistant Staphylococcus aureus ). The results reveal that TBPP-mediated BL-PDT shows outstanding bactericidal effects. Mechanism studies show that TBPP + BL can induce reactive oxygen species (ROS) up-regulated in MRSA, rupture cell membrane, inhibit ATP (adenosine triphosphate) production and virulence factor expression. Furthermore, TBPP + BL effectively eliminates MRSA form biofilms, inhibits biofilm formation and disintegrates mature biofilms. More importantly, TBPP-PDT significantly accelerate mouse skin wound healing in a biofilm infection model. Our work offers new insights into the development of novel BL photosensitizers. Photodynamic therapy mediated by 5,10,15,20-tetrakis(4-bromophenyl)porphyrin (TBPP) effectively eliminates drug-resistant Staphylococcus aureus , inhibits biofilm formation and accelerates wound healing in a mouse model.
研究领域:
微生物学
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药学
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生物医学工程
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传染病学
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2025
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IF 6.2
Wang Guo,Yiqing Guo,Hong Xu,Chen Li,Xinai Zhang,Xiaobo Zou,Zongbao Sun
Staphylococcus aureus (S. aureus) is a major pathogenic bacterium responsible for bacterial foodborne diseases, making its rapid, specific, and accurate detection crucial. In this study, we develop a ratiometric biosensor based on the recombinase polymerase amplification-clustered regularly interspaced short palindromic repeats/CRISPR associated protein 12a (RPA-CRISPR/Cas12a) system and Eu-metal–organic framework (Eu-MOF) fluorescent nanomaterials for the high-sensitivity detection of S. aureus, combining with RPA for efficient isothermal amplification, this sensor enhances specificity and sensitivity by utilizing the target activation of CRISPR/Cas12a. The Eu-MOF serves a dual function, providing stable red fluorescence as a reference signal and adsorbing FAM-labeled probes for fluorescence quenching, forming a dual-signal system that significantly reduces background interference. This ratiometric design enables accurate and quantitative detection over a wide range (7.9 × 100 to 7.9 × 108 CFU/mL) with a low detection limit of 3 CFU/mL. Overall, with these merits of simplicity, rapid response, high sensitivity, and specificity, this dual-signal biosensor offers a promising method for accurately evaluating S. aureus contamination in food under complex substrate conditions.
研究领域:
生物技术
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食品安全检测
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分子诊断
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Zhuohui Zhang,Yan Wang,Qianqian Xu,Yimiao Liu,Kaiyu Wang,Pishun Li,Xiaofeng Zheng
Methicillin-resistant Staphylococcus aureus (MRSA), one of the most important zoonotic bacterial pathogens, necessitates innovative antibacterial strategies. Rosmarinic acid (RA) possesses various biological functions, including antibacterial and anti-inflammatory effects. This study aimed to explore the synergistic effects and mechanism of RA in conjunction with ceftiofur (CF) against MRSA. Results showed that the combination of RA and CF demonstrated synergistic anti-MRSA activity, conferring protective effects in an MRSA bacteremia mouse model. Mechanistic investigations further established that RA interacts with sortase A (SrtA) through hydrogen bonds, electrostatic, and van der Waals forces at critical sites, leading to the reversible inhibition of SrtA activity. Consequently, this inhibition impeded the ability of MRSA to invade cells and form biofilms. Conclusively, RA directly inhibits SrtA activity, thus enhancing the synergistic anti-MRSA effect of CF. These findings provide a theoretical foundation for the use of RA in combination with CF as a therapeutic strategy for MRSA infections.
研究领域:
微生物学
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药理学
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感染病学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2025
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IF 9.8
Weipeng Tong,Yingjie Du,Mingjian Yao,Hao Fang,Weitao He,Yi Zhang,Yu Su,Yuankui Leng,Xiaolin Huang,Ying Xiong,Yonghua Xiong
An innovative light scattering immunoassay was developed using an AuNPs etching strategy. Three types of anisotropic gold nanoparticles, including gold nanocubes, nanorods, and nanoflowers with distinct morphologies, were utilized to investigate how these morphological differences affect the sensitivity of light scattering signal transduction. Based on theoretical insights into light scattering and electromagnetic fields, gold nanocubes were identified as the optimal probes for enhancing light scattering signal transduction and were employed to construct an immunoassay for detecting staphylococcal enterotoxin A (SEA). The developed immunoassay achieved ultrahigh sensitivity for SEA detection in milk samples, with a detection limit of 10.39 pg mL −1 , which is 190 times lower than that of conventional ELISA. The proposed immunoassay was validated across ten food samples, demonstrating high accuracy and robustness. Given these promising results, we believe this method has significant potential for screening trace levels of SEA in food products.
研究领域:
生物医学工程
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食品安全检测
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产品关联
DOI
PubMed
高影响因子
npj Science of Food
·
2022
·Vol. 6
·期 1
·1-11
·
IF 7
Chen Xuan,Wu Xiaoping,Wang Shaoyun
The misuse of antibiotics in animal protein production has driven the emergence of a range of drug-resistant pathogens, which threaten existing public health security. Consequently, there is an urgent need to develop novel antimicrobials and new infection treatment options to address the challenges posed by the dramatic spread of antibiotic resistance. Piscidins, a class of fish-specific antimicrobial peptides (AMPs), are regarded as promising therapies for biomedical applications. Progress towards potential analogs from the piscidin family has been hampered by unenforceable structural optimization strategies. Here, we leverage a strategy of bioinformatics analysis combined with molecular dynamics (MD) simulation to identify specific functional hotspots in piscidins and rationally design related analogues. As expected, this approach yields a potent and non-toxic PIS-A-1 that can be used as an antibiotic adjuvant to reverse methicillin-resistant Staphylococcus aureus (MRSA) pathogens. Remarkably, the structural optimization scheme and application strategy proposed here will contribute richer therapeutic options for the safe production of animal protein.
研究领域:
微生物学
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药学
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生物信息学
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分子生物学
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DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2022
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IF 10.383
Chengnan Li,Zongshao Li,Yingying Gan,Fenglin Jiang,Huimin Zhao,Jeremy Tan,Yi Yan Yang,Peiyan Yuan,Xin Ding
Antibiotic-free antimicrobial strategies are urgently needed to address the rapid evolution of antimicrobial resistance and transmission of multidrug-resistance bacterial infections. Herein, we fabricated polydopamine-coated porous magnetic nanoparticles (pMNPs@PDA) for effective separation and photothermal killing of methicillin-resistant Staphylococcus aureus (MRSA). Taking advantage of the excellent bacteria-affinitive property of polydopamine, the nanoparticles were anchored on the surface of bacteria, permitting rapid and efficient MRSA capture and separation with over 99% removal via the application of a magnetic field in 30 min. It was found, for the first time, that polydopamine-coated magnetic nanoparticles displayed a selective capture of Gram-positive bacteria when compared with Gram-negative bacteria. The selectivity was attributed to the preferable binding capability of pMNPs@PDA to peptidoglycan (PGN) of Gram-positive bacteria, compared to the lipopolysaccharide (LPS) of Gram-negative bacteria. With the magnetic separation and photothermal properties, pMNPs@PDA exhibited efficient killing of the captured MRSA under the irradiation of near-infrared (NIR) light. Cell cytotoxicity testing demonstrated good biocompatibility of the nanoparticles. These antibiotic-free nanoparticles capable of fast capture, separation, and inactivation of MRSA may be potentially used for water disinfection, blood purification, and treatment of bacterial infections.
研究领域:
纳米技术
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生物医学工程
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微生物学
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材料科学
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抗菌素耐药性
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DOI
PubMed
高影响因子
JOURNAL OF WUHAN UNIVERSITY OF TECHNOLOGY-MATERIALS SCIENCE EDITION
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2021
·Vol. 36
·期 6
·934-941
·
IF 0.957
Yan Chunchao,Xiao Feng,Qiu Tong,Zhang Xueqiong,Zhan Siwen,Lu Mengli,Yang Mengjia,Zhang Juan
To synthesize three different grafting ratios of gallic acid (GA)-chitosan (CS) copolymer by a free radical mediated grafting method, which is further applied to the field of antibacterial materials, crosslinking structures of the compound GA-CS copolymer were characterized, fully indicating that gallic acid is resoundingly grafted onto chitosan. The grafting ratios of three copolymers GA-CS are 45.71% (I), 36.12% (II), and 18.96% (III) were determined by UV-Vis spectrophotometer. The minimum inhibitory concentrations of three GA-CS copolymers are 30 µg/mL against Escherichia coli and are ranged from 250 to 550 µg/mL against Staphylococcus aureus. By counting viable bacterial colonies, it can be found that antibacterial property is preferable by increasing the grafting ratio of GA-CS copolymers. Findings of investigation on aforementioned bacteria experiment indicate that the CFU/mL values of GA-CS (I, II, III) are 2.04×10 6 , 7.56×10 6 , 1.48×10 7 to Staphylococcus aureus , and 2.96×10 6 , 1.01×10 7 , 2.14×10 7 to Escherichia coli after 12 h treatment. In addition, the interaction process between GA-CS copolymer and bacteria can be observed through a transmission electron microscope. The specific manifestation is that the bacterial cell membranes are ruptured after being treated with the copolymer, which causes the cell contents to flow out, and the cell morphology is shrunk and out of shape.
研究领域:
材料科学
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生物技术
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微生物学
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DOI
高影响因子
EMBO Molecular Medicine
·
2026
·Vol. 18
·期 5
·1990
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IF 7.9
Yini Huang,Yan Ye,Xinmei Zhu,Dengpan Liang,Ruiqin Cui,Xiaopeng Yuan,Xitao Li,Quanming Zou,Haibo Li,Wei Huang
The limitations of existing drugs and the development of drug resistance make it urgent to develop new drugs against methicillin-resistant Staphylococcus aureus (MRSA). The re-development of the antibacterial activity of drugs that have already been proven safe for human use is an effective way. In this study, we discovered that the Src homology region 2 domain-containing phosphatase-1 (SHP-1) agonist SC-43, exhibits potent activity against Gram-positive bacteria, including MRSA. The mode of action studies revealed that SC-43 inhibits the key enzyme coproporphyrin ferrochelatase (CpfC) of the coproporphyrin-dependent (CPD) heme synthesis pathway and interferes with the bacterial porphyrin metabolism. The determination of the structure of CpfC derived from S. aureus (SA CpfC ) in this study allowed us to reveal the inhibitory effect of SC-43 at the molecular level. Animal experiments showed that SC-43 has the potential to become a new anti-MRSA drug. In conclusion, this study discovered a new anti-MRSA activity of a drug currently undergoing clinical trials and simultaneously verified the feasibility of developing new anti-Gram-positive bacteria drugs by inhibiting the CPD pathway. The limitations of existing drugs and the emergence of drug resistance make the development of new therapies against methicillin-resistant Staphylococcus aureus (MRSA) increasingly urgent. Here, we demonstrate that the Src homology region 2 domain-containing phosphatase-1 (SHP-1) agonist SC-43 exhibits antibacterial activity against MRSA. The limitations of existing drugs and the emergence of drug resistance make the development of new therapies against methicillin-resistant Staphylococcus aureus (MRSA) increasingly urgent. Here, we demonstrate that the Src homology region 2 domain-containing phosphatase-1 (SHP-1) agonist SC-43 exhibits antibacterial activity against MRSA.
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DOI
PubMed
高影响因子
Food Analytical Methods
·
2021
·Vol. 14
·期 9
·1964-1976
·
IF 3.366
Chen Guanhua,Yang Guotai,Wang Yutong,Deng Mei,Wang Zhengzheng,Aguilar Zoraida P.,Xu Hengyi
Sensitive, simple, and rapid pathogen separation and detection methods are essential for the early diagnosis and immediate medical treatment of bacterial infections. In this study, a novel strategy based on vancomycin (Van)-modified ovalbumin (OVA) mediated magnetic nanoprobes (MBs-OVA-Van) combined with multiplex polymerase chain reaction (mPCR) was developed for efficient isolation and detection of Staphylococcus aureus ( S. aureus ) and Bacillus cereus ( B. cereus ). Van conjugated to ovalbumin coated magnetic microcarrier via strong covalent interaction. In addition, Van binds through hydrogen bonds with d -alanyl- d -alanine moieties on the surface of gram-positive bacteria cell wall. Under optimum conditions, the MBs-OVA-Van showed that the capture efficiency (CE) for S. aureus and B. cereus in PBS and juice samples were around 91%, 92% and 89%, 85%, respectively. In addition, with a mixture containing both target bacteria in PBS and juice samples, the CE for S. aureus and B. cereus were recorded at about 90%, 91% and 80%, 84%, respectively. The MBs-OVA-Van plus mPCR method applied in PBS and juice samples for both bacteria showed limits of detection at 10 2 CFU mL -1 in a < 3 h total assay time. The results indicated that the method holds promise for isolation and enrichment of gram-positive bacteria with S. aureus and B. cereus as models.
研究领域:
微生物学
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生物技术
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分子生物学
·
分析化学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2020
·
IF 5.162
Yinyin Liu,Zehao Li,Shuiyang Zou,Chunbo Lu,Yao Xiao,Hu Bai,Xiaoli Zhang,Haibo Mu,Xiuyun Zhang,Jinyou Duan
Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most common causes of hospital infection. Here, we showed that hyaluronic acid modified organic metal framework material ZIF-8 could be a Trojan horse of vancomycin (Van) for effective treatment of MRSA infections. The Van-loaded nanoparticles were readily up-taken by macrophages via a CD44-mediated process and collapsed in the acidic condition of endosome/lysosome, as a consequence, it could eradicate MRSA with high efficiency in macrophages. This drug delivery system with negligible toxicity could resolve MRSA infections in a well-established mouse pneumonia model.
研究领域:
药学
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纳米技术
·
传染病学
·
药物递送系统
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 187
·期 1
·1-10
·
IF 5.479
Zhang Xuzhi,Wang Xiaochun,Yang Qianqian,Jiang Xiaoyu,Li Yang,Zhao Jun,Qu Keming
A method is described to determine viable populations of Escherichia coli ( E. coli ) and Staphylococcus aureus ( S. aureus ). The method employs aptamer-magnetic separation combined with resistivity based detection. The bacteria were separated by means of aptamer-functionalized magnetic beads. They were then quantified by measuring their growth kinetics through time-dependent conductivity changes of culture media. The time-course of growth was logged by real-time and contactless measurements that yielded starting concentrations from the duration of lag intervals prior to the log phase of growth. In pure water samples, the linear ranges for measuring E. coli and S. aureus cells are 2.5 × 10 3 –2.5 × 10 8 CFU·mL −1 and 4.1 × 10 3 –4.1 × 10 8 CFU·mL −1 , respectively. In spiked tap water samples, the lower limits of detection are 2.3 × 10 4 CFU·mL −1 and 4.0 × 10 3 CFU·mL −1 for E. coli and S. aureus , with recoveries of 87.0–108.7% and 92.5–105.0%, respectively. The relative standard deviation of these measurements (10.0%) is below that of plate counting method (13.9%). The presence of micro/nanoparticles such as magnetic beads or selenium nanoparticles in the culture media does not interfere, unlike in case of automatted optical density monitoring. The E. coli and S. aureus cells captured on the aptamer-functionalized magnetic beads can be directly tested for their susceptibility to antibiotics. The process of magnetic separation and determination of load burden requires neither bulky, sophisticated equipment nor expensive reagents.
Aptamer-functionalized magnetic beads are used to selectively capture and separate E. coli and S. aureus cells in aqueous samples. They are directly transferred to a multichannel conductometric sensor for the quantification of viable bacteria via automated monitoring of their growth kinetics.
研究领域:
微生物学
·
分析化学
·
生物技术
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产品关联
DOI
PubMed
高影响因子
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