RSC Advances
·
2019
·Vol. 9
·期 58
·33589-33595
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IF 3.049
Yun Zhang,Shuyou Shi,Jiajia Xing,Wenqing Tan,Chenguang Zhang,Lin Zhang,Huan Yuan,Miaomiao Zhang,Jinjuan Qiao
In this study, a novel colorimetric sensing platform was developed for the detection of S. aureus using dog immunoglobulin G (IgG) as the capture antibody and chicken anti-protein A immunoglobulin Y labeled with horseradish peroxidase (HRP-IgY) as the detection antibody. Dog IgG labeled with magnetic beads was used to capture S. aureus through the interaction between the Fc region of dog IgG and Staphylococcal protein A (SPA). HRP-IgY was introduced to recognize the residual SPA on the surface of S. aureus and to create a sandwich format, after which a soluble 3,3′,5,5′-tetramethylbenzidine (TMB) substrate was added. A stop solution was utilized to cease the enzymatic chromogenic reaction, and then optical density was read at 450 nm. Under optimal conditions, the proposed method displayed a low detection limit of 1.0 × 103 CFU mL−1 and a wide linear range of 3.1 × 103 to 2.0 × 105 CFU mL−1. This detection method exhibited high specificity against other foodborne bacteria. The recovery rates ranged from 95.2% to 129.2%. To our knowledge, this is the first report to employ dog IgG and chicken IgY as an antibody pair to detect S. aureus. This technique exhibits high application potential for S. aureus monitoring in various kinds of samples.
研究领域:
生物传感器
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微生物学
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食品安全
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DOI
PubMed
高影响因子
ANALYTICAL BIOCHEMISTRY
·
2017
·
IF 2.334
Aicheng Wang,Huimin Zhao,Xiaochi Chen,Bing Tan,Yaobin Zhang,Xie Quan
A sensitive, rapid and label-free colorimetric aptasensor for sulfadimethoxine (SDM) detection was developed based on the tunable peroxidase-like activity of graphene/ [email protected] nanoparticle (Gr/ [email protected] ) hybrids. The addition of the SDM aptamer could inhibit the peroxidase-like catalytic activity of the hybrids. However, the target SDM and aptamer could be triggered tightly and recover the catalytic activity of the Gr/ [email protected] hybrids. Due to the peroxidase-like catalytic activity, Gr/ [email protected] could catalyze the decomposition of H 2 O 2 with releasing hydroxyl radicals which further oxidized reagent 3, 3′, 5, 5′-Tetramethylbenzidine (TMB) to oxTMB accompanied with a colorless-to-blue color change. The original color change could be applied to obtain quantitative detection of SDM, due to the relationship between the concentration of the target and the color difference. As a result, this approach performed a linear response for SDM from 1 to 500 ng/mL with a limit detection of 0.7 ng/mL (S/N = 3) under the optimized conditions and realized the detection of SDM in spiked lake water samples. Therefore, this colorimetric aptasensor was an alternative assay for SDM detection in real water. Moreover, with its design principle, this work might be applied to detecting other small molecule by employing appropriate aptamer.
研究领域:
分析化学
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纳米技术
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环境科学
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生物化学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Yingxia Zhou,Lai Wei,Lihua Chen
Herein, a small molecule, hemin/G-quadruplex (G4-hemin), was physically embedded into NH 2 -MIL-53 (Al) to construct a novel metal-organic framework (MOF)-based enzyme ( [email protected] ). Its catalytic activity and stability were investigated in detail by catalyzing a reaction of 3,3′,5,5′-Tetramethylbenzidine (TMB) in the presence of H 2 O 2 . The results show that the activity and stability of this new enzyme is better than that of traditional hemin or G4-hemin, and even stronger than that of [email protected] ( [email protected] > G4-hemin > [email protected] > hemin). No significant loss in catalytic activity was observed for at least one day. Even after eight days, only a 13.8 % decrease in activity was recorded. An electrochemical biosensor was successfully developed based on a signal amplification strategy utilizing the catalysis of [email protected] The sensor was able to sensitively detect TB in buffer, even in a diluted solution of many proteins. The proposed method offers high selectivity against mismatch sequences of TB, a good linear response range of 10 −18 moL L -1 to 10 -14 moL L -1 and a low minimum detection limit of 0.330 aM.
研究领域:
生物化学
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分析化学
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材料科学
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生物医学工程
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DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Yu Wang,Yu Xue,Qilin Zhao,Shuang Wang,Jian Sun,Xiurong Yang
Alzheimer’s disease (AD) is a common chronic neurodegenerative disease that manifests as cognitive impairment and behavioral deficits and severely threatens the health of the elderly. Acetylcholinesterase (AChE) plays a vital role in biological signaling and is an essential target for the early diagnosis and treatment of AD. Herein, 2D Zn-TCPP(Fe) nanosheets (NSs) employing Zn2+ and Fe-bound tetrakis(4-carboxyphenyl)porphyrin ligands were prepared through a surfactant-assisted synthetic method. The ultrathin two-dimensional (2D) metal–organic framework structures exhibited high peroxidase-like activity, which allowed the catalysis of the H2O2-initiated oxidation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to blue oxidized TMB (ox-TMB). Such catalytic performance inspired us to develop a convenient, rapid, and sensitive acetylcholinesterase activity assay, during which AChE can catalyze the substrate acetylthiocholine (ATCh) to produce thiocholine (TCh), and TCh could especially enable the degradation of 2D Zn-TCPP(Fe) NSs accompanied by the reduction of ox-TMB production. Our proposed sensing system exhibited favorable selectivity and sensitivity (LOD of 0.029 mU/mL) and has excellent potential to evaluate AChE activity in human serum samples and to screen AChE inhibitors. This colorimetric assay could provide an alternative pathway for early diagnosis and drug screening of AD, facilitating the development of AD therapy.
研究领域:
神经退行性疾病
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生物化学
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纳米技术
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诊断方法
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药物筛选
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2022
·
IF 12.545
Lei Wang,Ai Xu,Jing Yuan,Fan Jiang,Meixuan Li,Wuzhen Qi,Yanbin Li,Jianhan Lin
An hourglass-mimicking biosensor was developed to detect target bacteria in 15 mL centrifugal tube using immune magnetic nanobeads to isolate the target from large-volume sample, [email protected] nanozymes to label the target for amplification of biological signals, and a microplate reader/colorimetric card for determination of the target. First, a centrifugal tube with an iron ball framework was first coaxially assembled in the center of a Halbach ring magnet. After immune magnetic nanobeads, [email protected] nanozymes and bacterial sample were mixed by repeated bottom-up of the tube using a stepper motor , nanobead-bacteria-nanozyme complexes were formed. Then, colorless H 2 O 2 -TMB was catalyzed by the nanozymes to produce blue TMBox. The color change was finally analyzed using the microplate reader or colorimetric card to determine bacterial concentration . This hourglass-mimicking biosensor could separate ∼95% targets from 10 mL bacteria sample and detect targets from 1.6 × 10 1 to 1.6 × 10 6 CFU/mL in 1.0 h with low detection limit of 16 CFU/mL.
研究领域:
生物技术
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生物传感器
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微生物学
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分析化学
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DOI
PubMed
高影响因子
Analytical Methods
·
2022
·Vol. 14
·期 48
·5091-5099
·
IF 3.532
Yue Sun,Zihao Xie,Fubin Pei,Wei Hu,Shasha Feng,Qingli Hao,Bing Liu,Xihui Mu,Wu Lei,Zhaoyang Tong
The rapid spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) seriously threatened global public health. Establishing a rapid and sensitive diagnostic test for early detection of the SARS-CoV-2 nucleocapsid protein is urgently required to defend against the pandemic. Herein, an enhanced lateral flow immunoassay (LFIA) was fabricated by trimetallic Au@Pd@Pt core–shell nanozymes for detection of the SARS-CoV-2 nucleocapsid protein. The Au@Pd@Pt nanozymes (Au@Pd@Pt NZs) synthesized via a one-pot method, with a dendrite morphology and uniform particle size, showed excellent peroxidase-like activity. Due to the perfect enzyme-like catalytic activity toward 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide (H2O2), the catalytic signal could be generated even by a tiny amount of Au@Pd@Pt NZs accumulated on the test strip. Therefore, rapid detection with higher sensitivity was achieved. The Au@Pd@Pt NZs-based LFIA provided a quantitative range of 0.05–100 ng mL−1 with a limit of detection of 0.037 ng mL−1, which was 17-fold lower than the LFIA without enhancement. The average recoveries from spiked samples were in the range of 92.5–107.9% with relative standard deviations all less than 4%, indicating the reliability and repeatability of the proposed LFIA. Additionally, the proposed LFIA could report results within 30 min using a microplate reader. In conclusion, the Au@Pd@Pt NZs-LFIA is a rapid, simple, and sensitive method for detecting the SARS-CoV-2 nucleocapsid protein.
研究领域:
分析化学
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纳米技术
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病毒学
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生物医学工程
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Kaining Zhang,Hao Sun,Long Wei,Rui Hu,Hao Liu,Yongchao Lai,Xun Li
Glutathione (GSH) levels have been well validated to correlate with a variety of physiological and pathological conditions, such as malignancy, cardiovascular disease and aging, making the development of accurate, robust and sensitive GSH detection methods highly desirable. In this study, a novel metal–organic framework (MOF-Fe(DTNB))-based colorimetric method with a favorable dual-triggering function was proposed. MOF-Fe(DTNB) exhibits high peroxidase activity, which can catalyze the oxidation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to blue ox-TMB by hydrogen peroxide (H 2 O 2 ). This oxidation process of TMB can be inhibited not only by the reducing action of GSH, but also by the thiol exchange reaction between DTNB and GSH, in which the disulfide bond of DTNB in MOF-Fe(DTNB) is cleaved. Thus, with this dual triggering mechanism, the GSH concentration can be robustly measured in the MOF-Fe(DTNB)-derived colorimetric strategy. Significantly, this method is accurate (RSD < 6 %), selective and sensitive in biological plasma samples, with satisfactory recovery rates (96.7–103.3 %). It requires less instrumentation and has less interference from other substances. The linear range of the method is 0–80 µM, and the detection limit is as low as 0.28 µM. This dual-triggering MOF-Fe(DTNB)-derived colorimetric strategy has greatly simplified the GSH detection processes with improved accuracy, in both acidic and basic environments, which has potent applications in biochemical analysis and point-of-care testing.
研究领域:
生物化学
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分析化学
·
即时检测
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2020
·
IF 3.232
Wen Zheng,Jiahui Liu,Danyang Yi,Yadi Pan,Yijuan Long,Huzhi Zheng
Ficin has been reported to possess peroxidase activity, but its applications in some respects have been limited because of its relatively low activity. Herein, a mesoporous metal-organic framework, PCN-333(Fe), was synthesized, which was selected to encapsulate ficin to form [email protected] (Fe). Compared with ficin, the peroxidase-like activity of [email protected] (Fe) toward 3,3′,5,5′-tetramethylbenzidine (TMB) oxidation was about 3 times increase in the presence of H 2 O 2 , and followed classical Michaelis-Menten model. The kinetic parameters showed that stronger affinity and higher catalytic constant ( K cat ) of [email protected] (Fe) to both TMB and H 2 O 2 compared with ficin, and K cat of [email protected] (Fe) was increased by 3.65 folds and 3.59 folds for TMB and H 2 O 2 , respectively. Taking advantages of higher catalytic property of [email protected] (Fe), we developed a colorimetric method with high sensitivity and selectivity to detect glucose, which displayed a good linear response toward glucose in the range of 0.5–180 μM with a limit of detection of 97 nM. Furthermore, [email protected] (Fe) has been proven to successfully detect glucose in human serum, implying its great potentialities and wide applications as peroxidase mimics.
研究领域:
生物化学
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分析化学
·
材料科学
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DOI
PubMed
高影响因子
TALANTA
·
2018
·
IF 4.244
Menglu Li,Juan Yang,Yining Ou,Ying Shi,Li Liu,Chaoqun Sun,Huzhi Zheng,Yijuan Long
The peroxidase-like activity of 2',7'-difluorofluorescein (DFF), was investigated using 3,3',5,5'-tetramethylbenzidine (TMB) as a chromogenic substrate in the presence of H 2 O 2 . DFF could catalyze oxidization of TMB by H 2 O 2 to produce a blue colored oxidation product. Effect of various reaction conditions, such as pH, temperature, H 2 O 2 concentration and reaction time on the catalytic activity of DFF was studied. The peroxidase-like activity of DFF was found to follow Michaelis–Menten kinetics, and its catalysis accorded with ping-pong mechanism. The calculated kinetic parameters (K cat ) of DFF catalysis showed higher peroxidase-like activity than fluorescein and 2′,7′-dichlorofluorescein (DCF). According to the radical capture and electron spin resonance (ESR) spectroscopy results, we confirmed that hydroxyl radical (•OH) is the active specie of catalytic process. It is known that the oxidation of galactose by galactose oxidase (GAOx) enzyme leads to the formation of H 2 O 2 , the H 2 O 2 released in this reaction was consequently quantified using DFF as peroxides mimics and TMB as the chromogen. Thus, a combination of above two reactions was exploited to establish a method for galactose detection. Under the optimum conditions, the linear range of this method was from 10 μM to 20 mM with the detection limit down to 3 μM. Moreover, the developed method was applied to detect galactose in urine samples. Our work will facilitate the utilization of DFF intrinsic peroxidase-like activity in medical diagnostics and biotechnology.
研究领域:
生物化学
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分析化学
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医学诊断
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生物技术
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Feiyang Wang,Na Na,Jin Ouyang
A multidimensional colorimetric sensor array was produced based on a catalytic – regulated gold nanorod (Au NR) etching process as a receptor for protein detection. With the peroxidase-like property of gold nanosphere (Au NP) and H 2 O 2 , 3,3′,5,5′-tetramethylbenzidine (TMB) (colorless) is oxidized into TMB 2+ (yellow). Different concentrations of TMB 2+ promote different degrees of Au NR etching, and the localized surface plasmon resonance band of the Au NR is blueshifted. The blueshift results in color displays (gray, purple, blue and pink), which can be clearly distinguished by the naked eye. When the surfaces of the Au NP are covered by proteins, the catalytic activity is influenced. On this basis, the dynamically tunable system obtains triple channel information including the plasma resonance effect, the catalytic activity of Au NP and the etching of Au NR by TMB 2+ (catalytic products of TMB in the Au NP-H 2 O 2 reaction system), which can be adjusted using different proteins. The array can correctly identify native and thermally denatured proteins. This may provide a new method for studying conformational changes in biological macromolecules. Furthermore, pure protein (different concentrations) and binary protein mixtures can be efficiently differentiated in the urine sample.
研究领域:
纳米技术
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分析化学
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生物传感器
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蛋白质科学
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生物物理学
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产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2020
·
IF 9.038
Yu Mao,Shengjie Gao,Lili Yao,Lu Wang,Hao Qu,Yuen Wu,Ying Chen,Lei Zheng
As a high biologically toxic heavy metal ion , Cr(VI) will cause environmental pollution and endanger human health. Therefore, the development of fast, simple and visible detection methods for Cr(VI) is extremely important to control its harm. Toward this end, we report the establishment of a colorimetric sensing method for Cr(VI) based on single-atom nanozymes for enhanced detection performance. Firstly, we prepared SA-Fe/NG as peroxidase mimetic by anchoring Fe single-atom onto a single-layer of two-dimensional nitrogen-doped graphene. The SA-Fe/NG showed superiorly high oxidation catalytic activity due to its 100% atomic utilization and existing Fe-N-C structure. Furthermore, with 3,3′,5,5′-tetramethylbenzidine (TMB) as a colorimetric sensing probe, and 8-hydroxyquinoline (8-HQ) as an inhibitor for the oxidation of TMB, the detection of Cr(VI) was realized through specific interaction between Cr(VI) and 8-HQ, which led to the recovery of oxTMB in blue color. Our established method showed superior sensitivity with a detection limit of 3 nM and a linear range of 30 nM to 3 μM. It also exhibited high selectivity for a series of metal cations, and has been successfully applied to the detection of Cr(VI) in tap water and tuna samples.
研究领域:
环境科学
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分析化学
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纳米技术
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Xue Wang,Futing Wen,Liu He,Jiangyu Su,Ping Jiang,Daiping He
Defects engineering as an effective strategy was widely utilized to modulate catalytic performance for heterogeneous catalysts through tuning surface electronic surface and creating more active sites. In this work, porous Co–Mn oxide nanosheets with abundant oxygen vacancies (OVs-CMO NSs) were prepared via a simple “one-stone-two-birds” method. OVs were verified by various characterizations and found more favorable for O 2 absorption and activation to produce more reactive O2•- radical, resulting in an improved oxidase-like activity in initiating 3,3′,5,5′-tetramethylbenzidine (TMB) oxidation , with a lower K m value of 0.00717 mM and a higher V max of 2.086 × 10 −7 M/s. Since heparin (Hep) shows an inhibition effect on OVs-CMO NSs-TMB chromogenic system due to strong absorption, a colorimetric sensing platform for Hep detection was established. The present colorimetric detection shows high sensitivity, good selectivity and reliability for normal goat serum. This work not only provides a high-performance non-precious metal oxidase mimic prepared by simple surface defect engineering, but also builds a sensitive colorimetric method for Hep detection.
研究领域:
材料科学
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纳米技术
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分析化学
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生物化学
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产品关联
DOI
PubMed
高影响因子
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