LANGMUIR
·
2025
·
IF 3.9
Qintao Yang,Zechao Zheng,Chenhua Shu,Qinglin Xie,Xiangpeng Gao
In this study, a novel As(V) ion-imprinted and 4-picolinic acid (4-PA)-grafted porous diatomite (DE)-based adsorbent (As(V)-IID) was prepared by using the surface ion imprinting technique. The results of the selective adsorption experiment show that the adsorption capacity of As(V) on As(V)-IID reaches 62.78 mg/g in a solution with an initial As(V) concentration of 25 mg/L and a pH value that is near pH 4.0 of the imprinting. The selectivity coefficients (K) of As(V) with respect to Cr(VI) and Mo(VI) were up to 12.14 and 27.79, respectively. The binding process was described well by the pseudo-second-order kinetics and Weber–Morris and Langmuir models. Fourier transform infrared spectra (FTIR), scanning electron microscopy energy dispersive X-ray spectroscopy (SEM-EDS), and X-ray photoelectron spectroscopy (XPS) analyses suggested that the nitrogen of secondary amide and pyridine was the main imprinting site and played a crucial role in the selectivity of binding of As(V) to As(V)-IID. To further validate the sorption mechanisms, density functional theory (DFT) was employed to study the properties of binding of As(V), Cr(VI), and Mo(VI) with As(V)-IID. The computational results showed that the As(V) was selectively recognized by complexing with N-containing functional groups of the As(V)-IID surface into a tridentate mononuclear configuration with a minimal binding energy (Eb) of −18.16 eV, which was completely consistent with characterization analyses. This work revealed the selective adsorption mechanisms of As(V)-IID for As(V) and provided new insight into the selective recognition of oxyanions from water by ion-imprinted adsorbents.
研究领域:
环境科学
·
材料科学
·
化学工程
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分析化学
·
物理化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
·
2024
·
IF 3.4
Caixia Pei,Xinlv Peng,Yiran Wu,Runmiao Jiao,Tiehai Li,Siming Jiao,Lei Zhou,Jianjun Li,Yuguang Du,Eika W. Qian
Eukaryotic sialyltransferases play key roles in many physiological and pathological events. The expression of active human recombinant sialyltransferases in bacteria is still challenging. In the current study, the genes encoding human N -acetylgalactosaminide α2,6-sialyltransferase V (hST6GalNAc V) and N -acetylgalactosaminide α2,6-sialyltransferase VI (hST6GalNAc VI) lacking the N -terminal transmembrane domains were cloned into the expression vectors, pET-32a and pET-22b, respectively. Soluble and active forms of recombinant hST6GalNAc V and hST6GalNAc VI when coexpressed with the chaperone plasmid pGro7 were successfully achieved in Escherichia coli . Further, lactose (Lac), Lacto- N -triose II (LNT II), lacto- N -tetraose (LNT), and sialyllacto- N -tetraose a (LSTa) were used as acceptor substrates to investigate their activities and substrate specificities. Unexpectedly, both can transfer sialic acid onto all those substrates. Compared with hST6GalNAc V expressed in the mammalian cells, the recombinant two α2,6-sialyltransferases in bacteria displayed flexible substrate specificities and lower enzymatic efficiency. In addition, an important human milk oligosaccharide disialyllacto- N -tetraose (DSLNT) can be synthesized by both human α2,6-sialyltransferases expressed in E. coli using LSTa as an acceptor substrate. To the best of our knowledge, these two active human α2,6-sialyltransferases enzymes were expressed in bacteria for the first time. They showed a high potential to be applied in biotechnology and investigating the molecular mechanisms of biological and pathological interactions related to sialylated glycoconjugates.
研究领域:
分子生物学
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生物化学
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生物技术
·
酶学
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糖生物学
✓ 已验证
DOI
PubMed
高影响因子
Analytical Methods
·
2023
·Vol. 15
·期 15
·1923-1931
·
IF 3.1
Zeyi Li,Yunrui Cao,Tingyu Feng,Tingting Wei,Changhu Xue,Zhaojie Li,Jie Xu
To better satisfy the application of rapid detection methods in the detection of As(V) in complex food substrates, we developed an “off–on” fluorescence assay to detect As(V) based on the competition between the electron transfer effect of nitrogen-doped carbon dots (N–CDs)/Fe3+ and the complexation reaction of As(V)/Fe3+, using N–CDs/Fe3+ as a fluorescence probe. Solid-phase extraction (SPE) was used to eliminate matrix interference during sample pretreatment. The detection limit was 7.6 ng g−1, with a linear range of 10–100 ng g−1. The method was further used to determine As(V) in different seafood products including snapper, shrimp, clams, and kelp. At the same time, the recovery of the method was validated by high-performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP/MS), indicating that the developed method had good recoveries from 86% to 117% and met the needs for accurate determination of As(V). This approach has shown excellent application potential in the field of As(V) detection in various seafood products.
研究领域:
分析化学
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环境科学
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食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2019
·
IF 0
Jiaxing Wei,Mohamed F. Aly Aboud,Imran Shakir,Zhangfa Tong,Yuxi Xu
The application of sorbent in solving the water pollution induced by heavy metals or metalloids is severely hindered by poor sorption performance and high cost, especially in simultaneous removal. Design and synthesis of nanocomposites as effective sorbents has been widely considered as a promising approach to address this problem. Herein, graphene oxide supported organo-montmorillonite composite (GO-OM) was prepared through a facile ultrasonic-assisted method for the first time and exhibited excellent synergetic adsorption behavior for mixed pollutants in aqueous solutions. Unlike previous studies, the simultaneous removal process can be well-described by the Freundlich and pseudo-second-order kinetic models. The sorption capacities of GO-OM are very high for both metal cations and oxyanionic groups, showing excellent potential in the removal of multiple contaminants. Our study also reveals that GO-OM has high recycling efficiency and good performance under a low level concentration. All results suggest that GO-OM can be used as effective and ecofriendly sorbents for the removal of combined pollutants in an aqueous environment. This work contributes to developing high-performance graphene materials with organic minerals and gives deep insight into simultaneous sorption of metal ions, which can be potentially extended to other multiple pollutants.
研究领域:
环境科学
·
材料科学
·
水处理
✓ 已验证
产品关联
DOI
Environmental Science-Nano
·
2019
·Vol. 6
·期 5
·1406-1417
·
IF 7.704
Yili Xie,Chen Tian,Weiyi Chen,Can Wu,Zhangbin Liu,Ping Ning,Hong Deng,Zhang Lin
Manganese ferrite (MnxFe3−xO4) microspheres with a spinel structure are very effective adsorbents for arsenic (As) removal. In this study, highly ordered mesoporous MnxFe3−xO4 has been synthesized by a facile and convenient solvothermal method. By comparing the different doping amounts of manganese (Mn), the results show that Mn1.8Fe1.2O4 has a relatively higher efficiency for arsenate (As(V)) removal due to the modified surface complexes caused by the optimum Mn substitution. Rietveld XRD refinement reveals that Mn1.8Fe1.2O4 microspheres possess the highest distribution ratio of Mn/Fe situated between the octahedral (Oh) and the tetrahedral (Td) sites of the unit cell. The as-prepared Mn1.8Fe1.2O4 microspheres with MnO6Oh sites in Fdm symmetry exhibit a promising ability for toxic As(V) adsorption, which is mainly attributed to the increase of coordinated complexes. Extended X-ray absorption fine structure (EXAFS) analysis indicates that the coordination structures of As(V) on Mn1.8Fe1.2O4 are present as bidentate binuclear complexes with a regular distance of As–Fe = 3.41 Å. Apart from this, As(V) can also bind with Mn-doped sites through the monodentate coordination mode at a distance of As–Mn = 3.51 Å with a preferred coordination number (CN) of 4.4. This work discloses the correlation between the superior As(V) adsorption ability and MnO6Oh sites in Mn1.8Fe1.2O4 and provides an emerging and promising method to enhance the adsorption capacity of As via modifying Mn doping sites based on the pollutant structure to achieve synergistic adsorption effects.
研究领域:
环境科学
·
材料科学
·
化学
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2018
·
IF 6.735
Kunlun Yang,Jiasheng Zhou,Zimo Lou,Xurui Zhou,Yuanli Liu,Yizhou Li,Shams Ali Baig,Xinhua Xu
Fe-Mn binary oxides (FMBO) with different Fe/Mn molar ratios were synthesized to investigate the influence of the changed iron oxides forms and the manganese oxide on the removal efficacy of Sb(V) from water under neutral pH. The characterization tests by electron microscopy and X-ray diffraction spectroscopy showed that FMBO gradually changed from granular to aciculiform or fibrous structure and their crystalline degree increased with the decreasing Mn content. The ferric oxides in FMBO transformed from 2-L ferrihydrite-like substance with amorphous state to goethite or lepidocrocite with high crystalline as the amount of added Mn reduced during the preparation. The Sb(V) adsorption rates and capacities of FMBO both decreased and the influence of pH on the Sb(V) removal became greater with the decreasing Mn oxide content. All the results indicated that the addition of Mn could greatly improve the Sb(V) removal performance of FMBO through promoting the formation and preventing the transformation of amorphous ferric oxide in FMBO during the preparation, whose Sb(V) adsorption ability was confirmed to be stronger than that of goethite or lepidocrocite. Further exploration indicated the complexation reaction between Sb(V) and amorphous ferric oxide played a key role in Sb(V) adsorption by FMBO. Meanwhile, FMBO with a Fe/Mn molar ratio of 3:1 (FMBO 3 ) exhibited excellent regeneration performance and the amorphous iron oxide in FMBO 3 gradually transformed into crystalline iron oxide during the consecutive recycling.
研究领域:
环境科学
·
水处理
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
·
2021
·
IF 3.493
Xiaoting Dong,Pengli Xue,Xian Ma,Yifan Bai,Penghui Shi,Liujiao Bian
The recognition and interaction of FEZ-1 from Legionella (FEZ-1) with penicillin V(PV) and cefoxitin(CFX) were investigated using fluorescence spectra in combination with molecular dynamics simulation (MD). The results revealed that the CFX bind with FEZ-1 in stronger interaction and induced larger conformational change than PV, despite all being forced by the electrostatic interaction and along with the changing in an environment of amino acid residues as well as the polypeptide skeleton inside the FEZ-1. Moreover, only the loop1, loop2, and N-terminal were observed locating near the binding pocket of FEZ-1, consisting of a flexible “gate-like” zone with better adaptability that controlled the entrance of antibiotic into the pocket by allowing the newly introduced antibiotic to match the pocket better through the conformational changes of these three substructures in the binding procedure. The current study may provide some valuable information on the antibiotic hydrolytic process by metallo-beta-lactamase and thus the references for the development of new antibiotics for super bacteria.
研究领域:
微生物学
·
药理学
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生物物理学
·
计算生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2022
·
IF 8.943
Heng Li,Kaisheng Gong,Xiaoying Jin,Gary Owens,Zuliang Chen
Since antimony (Sb) is a toxic metalloid cost-effective method for the simultaneous removal of the two major Sb species from mining wastewater has attracted much attention. In this study, phytosynthesized iron nanoparticles ( n Fe) prepared using a eucalyptus leaf extract were successfully used to simultaneously remove Sb(III) and Sb(V) via an adsorption and oxidation mechanism with removal efficiencies of 100 and 97.7% for Sb(III) and Sb(V), respectively. Advanced analysis using X-ray photoelectron spectroscopy (XPS), ion chromatography-atomic fluorescence spectroscopy (IC-AFS), and electrochemical analysis confirmed that Sb(III) was oxidized to Sb(V) by Fe(III) on the n Fe surface while Scanning Electron Microscopy (SEM) and Energy Dispersive Spectroscopy (EDS) indicated that both Sb(III) and Sb(V) were adsorbed onto n Fe. Adsorption of both Sb(III) and Sb(V) best fit the Langmuir adsorption model with R 2 of 0.999 and 0.989, respectively and both followed pseudo-second-order kinetics with R 2 of 0.999 and 0.981, respectively. Furthermore, the adsorption rate of Sb(III) was faster than that of Sb(V) due to inner-sphere complex formation, and the Fe–O bonds in the asymmetric tetrahedron structure of Sb(III) were easier to break due to a lower energy barrier (0.863 eV). Consequently, a simultaneous removal mechanism of Sb(III) and Sb(V) was proposed. Finally, n Fe was used practically to remove Sb in mining wastewater with a removal efficiency of 93.5%, demonstrating that n Fe have significant potential to remove Sb in contaminated mining wastewaters.
研究领域:
环境科学
·
纳米技术
·
水处理
·
材料科学
✓ 已验证
DOI
高影响因子
CHEMICAL ENGINEERING SCIENCE
·
2023
·
IF 4.7
Tunmise Ayode Otitoju,Yun Bai,Yaxiong Tian,Zhongmin Feng,Yun Wang,Xia Zhang,Ting Sun
Herein, we present a novel polyvinylidene fluoride/vanadium-titanium dioxide (PVDF-TiO 2 /V) membranes prepared using LBL self-assembly. The membranes were tested for ultrafiltration of bovine serum albumin solution and photodegradation of SMX solution. The neat PVDF membrane was endowed with photocatalytic function due to the synergistic interaction of TiO 2 and V. The structure and morphology of PVDF-TiO 2 /V membranes were characterized using a variety of techniques, showing that TiO 2 /V particles had been successfully integrated on the membrane surface. By increasing the number of TiO 2 /V layers, contact angle tests demonstrated that the membranes hydrophilicity has been significantly improved. The FTIR result demonstrated that the TiO 2 /V was affixed to the surface of the PVDF membrane by interfacial interactions. The thermal property of the PVDF membrane was also improved with the assembly of TiO 2 /V on the membrane surface. The PVDF-TiO 2 /V membranes maintained a high BSA rejection of 99.4 % and FRR of 97.4 % while showing a water flux up to 390.07 kg m -2 h −1 , which is ∼1.5 times higher than that of neat PVDF membrane. Specifically, the membranes were capable of degrading SMX under visible-light. The photocatalytic activity for SMX increased with the number of TiO 2 /V layers, attaining the highest value with four self-assembled TiO 2 /V layers. The reaction rate of the optimum membrane was 11.57-fold compared to the neat membrane under visible-light. Furthermore, 95.9 % degradation efficiency was achieved, even after six cycles. More importantly, BPA, MO, and MB could be photodegraded using the PVDF-TiO 2 /V membranes. Compared to using bulk or powdered photocatalysts, the PVDF-TiO 2 /V membrane is more practical and promising for wastewater remediation due to its many advantages including better long-term stability, ability to endure pollutant degradation, low cost, and environmentally friendly.
研究领域:
环境科学
·
材料科学
·
水处理
✓ 已验证
产品关联
DOI
高影响因子
FREE RADICAL BIOLOGY AND MEDICINE
·
2020
·
IF 6.17
Xu Zhang,Wei Xiong,Ling-Li Chen,Jia-Qiang Huang,Xin Gen Lei
Selenoprotein V (SELENOV) contains a thioredoxin-like fold and a conserved CxxU motif with a potential redox function. This study was to assess its in vivo and in vitro roles and mechanisms in coping with different oxidant insults. In Experiment (Expt.)1, SELENOV knockout (KO) and wild type (WT) mice (male, 8-wk old) were given an ip injection of saline, diquat (DQ, 12.5 mg/kg), or N -acetyl- para -aminophenol (APAP, 300 mg/kg) (n = 10), and killed 5 h after the injection. In Expt. 2, primary hepatocytes of WT and KO were treated with DQ (0–0.75 mM) or APAP (0–6 mM) for 12 h. In Expt. 3, 293 T cells overexpressing Selenov gene (OE) were treated with APAP (0–4 mM) for 24 h or H 2 O 2 (0–0.4 mM) for 12 h. Compared with the WT, the DQ- and APAP-injected KO mice had higher (P < 0.05) serum alanine aminotransferase activities and hepatic malondialdehyde (MDA), protein carbonyl, endoplasmic reticulum (ER) stress-related proteins (BIP and CHOP), apoptosis-related proteins (FAK and caspase-9), and 3-nitrotyrosine, along with lower total anti-oxidizing-capability (T-AOC) and severer hepatic necrosis . Likewise, the DQ and APAP-treated KO hepatocytes had elevated (P < 0.05) cell death (10–40%), decreased (P < 0.05) T-AOC (63–83%), glutathione (26–87%), superoxide dismutase (SOD) activity (28–36%), mRNA levels of redox enzymes ( Cat , Gcs, Gpx3, and Sod ) and (or) sharper declines (P < 0.05) in cellular respiration and ATP production than that of the WT cells. In contrast, the OE cells had greater viability and T-AOC and lower MDA, and carbonyl contents after the APAP and H 2 O 2 exposures (all at P < 0.05) than the controls. Moreover, the OE cells had greater (P < 0.05) redox enzyme activities (GPX, TrxR, and SOD), and lower (P < 0.05) expressions of ER stress-related genes (Atf4, Atf6, Bip, Xbp1t, Xbp1s , and Chop ) and proteins (BIP, CHOP, FAK, and caspase-9) than the control cells after the treatment of H 2 O 2 (0.4 mM). In conclusion, SELENOV conferred protections in vivo and in vitro against the reactive oxygen and nitrogen species-mediated ER stress-related signaling and oxidative injuries.
研究领域:
生物化学
·
细胞生物学
·
药理学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Hongtao Liu,Zekai Qin,Xueli Wang,Maolin Tian,Wenjie Zhang,Lin Li,Jiawei Wen,Guoyong Huang,Shengming Xu
Spent hydrodesulfurization catalysts contain more than 10 ∼ 25 wt% V, Mo and Co, which are not only hazardous materials, but also can be considered as valuable secondary resources. The complete separation of these complex critical metals by solvent extraction still needs to be improved. Here, a selective extraction route for the separation of V/Mo/Co is proposed based on the weak interaction differences between extractants and metal, which is realized by theoretical calculation and experimental verification. This process does not require pretreatment of N1923 and Cyanex272 (C272), thus reduced the consume of acid and base as well as generation of waste water. With the preliminary results of V/Mo, Co/Mo, and V/Co separation, the weak interaction between extractants and metal molecules was revealed by quantum chemical calculations quantitatively. The molecular charge distribution, surface electrostatic potential distribution (ESP) and the average local ionization energy (ALIE) shows the priority reactivity of the metal molecules as: H 6 V 10 O 28 > H 2 Mo 4 O 13 > H 4 V 4 O 12 > H 3 VO 4 > H 2 MoO 4 . The independent gradient model based on Hirshfeld partition (IGMH) show differences in the weak interaction forces as: N1923-V>C272-Mo > C272-V>N1923-Mo > C272-Co. The selective separation process of V/Mo/Co was designed with the guide of above results. The combined recovery of the three metals V, Mo and Co amounted to 83.88 %, 87.08 %, and 99.01 %, respectively. The whole route reduces 3.92 t sulfuric acid and 7.83 t sodium hydroxide per solid, and reduces 1.99 t carbon emissions compared to the conventional route.
研究领域:
化学工程
·
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
ACS Sustainable Chemistry & Engineering
·
2025
·
IF 7.3
Xueting Li,Guimin Wang,Chunmei Lv,Xiuwen Wang,Yue Liu,Haijing Yan,Zilin Zhang,Yanqing Jiao
Efficient non-Pt electrocatalysts for the alkaline hydrogen evolution reaction (HER) are necessary for industrial water electrolysis. Cu-based materials are cheaper alternatives, but their performances are far from satisfactory due to slow water dissociation kinetics and weak Gibbs free energy of hydrogen adsorption (ΔGH*). Herein, we propose to construct multiple sites with different activation functions in response to sluggish alkaline HER kinetics. A multi-interfacial, ultrasmall V-doped MoP and Cu3P heterostructure (V-MoP/Cu3P) was successfully fabricated by confined conversion of a V–Mo bimetallic polyoxometalate (POM) cluster embedded in a Cu-based metal–organic framework (MOF). The spatial separation of each POM cluster by the MOF and the inherent structure of each [VO6] connecting four [MoO6] octahedrons enable precise V incorporation in the MoP lattice and multi-interface coupling of the MoP and Cu3P heterojunction. As a result, the interfacial charge redistribution occurs and the d-band centers are finely tuned, triggering a multisite synergism to encourage the adsorption and activation of H2O, H–OH* bond breaking, and H2 release. Consequently, V-MoP/Cu3P exhibits significantly enhanced HER activity, even superior to the commercial 20% Pt/C catalyst at current densities exceeding 155 mA cm–2, which indicates the feasibility of the V-MoP/Cu3P catalyst as cheaper alternatives to Pt-based catalysts in industrial applications. This work provides new insight for the design of low-cost, high-abundance HER catalysts via multi-interface engineering and heteroatom doping to trigger multisite synergies.
研究领域:
电化学
·
催化
·
材料科学
·
纳米技术
·
能量转换
·
表面科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）