Bioactive Materials
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2025
·
IF 20.3
Renwen Wan,Zhiheng Lin,Mowen Xu,Wei Luo,Hengjie Jia,Zhufeng Hu,Zhengyuan Fang,Junming Sun,Yisheng Chen,Shiyi Chen,Zhiwen Luo,Lei Yi,Zhijie Zhao
Diabetic wound healing is a significant complication of diabetes mellitus, characterized by prolonged healing times, heightened infection risks, and potential amputations, necessitating innovative therapeutic approaches. This study aims to evaluate the efficacy of a novel injectable hydrogel, AP@HA-Si InjGel, which combines hyaluronic acid, silanol, and bioactive compounds to promote wound healing in diabetic patients. Utilizing a combination of in vitro assays, diabetic mouse models, and advanced techniques such as single-cell RNA sequencing, we demonstrated that AP@HA-Si InjGel significantly accelerates wound closure, enhances collagen deposition, and modulates macrophage polarization towards a pro-healing M2 phenotype while suppressing the inflammatory M1 phenotype. The hydrogel exhibited excellent biocompatibility with over 90 % cell viability and significant antioxidant properties, reducing reactive oxygen species accumulation. Histological analysis confirmed that AP@HA-Si InjGel promoted neovascularization and improved extracellular matrix remodeling, thereby enhancing tissue regeneration. Furthermore, single-cell RNA sequencing revealed distinct macrophage subtypes, with an increased proportion of M2 macrophages and a favorable differentiation trajectory towards enhanced wound healing. Overall, these findings highlight the therapeutic potential of AP@HA-Si InjGel as a comprehensive strategy for diabetic wound management, warranting further exploration in clinical settings to address the growing challenge of impaired wound healing in diabetic patients.
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高影响因子
BIOMATERIALS
·
2014
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IF 8.312
Dong Ma,Qian-Ming Lin,Li-Ming Zhang,Yuan-Yuan Liang,Wei Xue
The co-delivery of drug and gene has become the primary strategy in cancer and other disease therapy. To co-deliver hydrophobic drug and functional gene efficiently into tumor cells, a star-shaped copolymer (PP-PLLD-Arg) with a photochemical internalization effect consisting of a porphyrin (PP) core and arginine-functionalized poly( l -lysine) dendron (PLLD-Arg) arms has been designed, and used to co-deliver docetaxel (DOC) and MMP-9 shRNA plasmid for nasopharyngeal cancer therapy. It was found that PP-PLLD-Arg/MMP-9 nanocomplex showed the photo-enhanced gene transfection efficiency in vitro , and could mediate a significant reduce of MMP-9 protein expression in HNE-1 cells. For co-delivery analysis, the obtained PP-PLLD-Arg/DOC/MMP-9 complexes could induce a more significant apoptosis than DOC or MMP-9 used only, and decreased invasive capacity of HNE-1 cells. Moreover, the star-shaped copolymer exhibited better blood compatibility and lower cytotoxicity compared to PEI-25k in the hemolysis and MTT assays , and also showed a good biocompatibility in vivo . Therefore, PP-PLLD-Arg with suited irradiation is a promising non-toxic and photo-inducible effective drug and gene delivery strategy, which should be encouraged in tumor therapy.
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FISH PHYSIOLOGY AND BIOCHEMISTRY
·
2019
·Vol. 45
·期 2
·539-549
·
IF 1.729
Chen Jiaojiao,Zhang Dianfu,Tan Qingsong,Liu Mengmei,Hu Pengcheng
Dietary arginine (Arg) could improve the intestinal structure and absorption of grass carp ( Ctenopharyngodon idellus ); however, the mechanism of Arg on intestinal morphology improvement was unclear. The present study aimed to explain the possible mechanism of the positive effect of Arg on intestinal epithelial cells of grass carp. An in vitro study was conducted through a primary culture model to assess the growth, cell viability, mRNA expressions of TOR signal pathway, and tight junction proteins of enterocytes after culture in the medium with 6 levels of Arg (0, 0.1, 0.2, 0.5, 1.0, and 2.0 mmol/L). The results showed that 0.5 mmol/L Arg improved the cell number and decreased the lactate dehydrogenase and creatine kinase activities in culture medium ( P < 0.05). The alkaline phosphatase activity in cell lysis buffer was depressed by 1 and 2 mmol/L Arg ( P < 0.05). The nitric oxide (NO) content showed an increasing trend with the Arg content ( P < 0.05), whereas the NO synthase activity showed an opposite trend to NO. TOR expression was higher in 0.2 and 0.5 mmol/L groups, whereas S6K1 expression in 1.0 mmol/L and 2.0 mmol/L groups were lower ( P < 0.05). The mRNA expressions of occludin, claudin 3, and claudin c in 0.5 mmol/L group were the highest, while ZO-1 and claudin b expressions were higher in 0.2 and 0.5 mmol/L groups ( P < 0.05). This study indicated that Arg enhanced the growth and integrity of intestinal epithelial cells of grass carp through upregulation of mRNA expression of TOR signal pathway and tight junction proteins at an optimal Arg content of 0.2–0.5 mmol/L.
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高影响因子
Journal of Biological Engineering
·
2021
·Vol. 15
·期 1
·1-16
·
IF 4.355
Fu Chuan,Qi Zhiping,Zhao Chengliang,Kong Weijian,Li Hongru,Guo Wenlai,Yang Xiaoyu
Skin wound healing is a complicated and lengthy process, which is influenced by multiple factors and need a suitable cellular micro-environment. For skin wound, wound dressings remain a cornerstone of dermatologic therapy at present. The dressing material can create an effective protective environment for the wound, and the interactions between the dressing and the wound has a great impact on the wound healing efficiency. An ideal wound dressing materials should have good biocompatibility, moisturizing property, antibacterial property and mechanical strength, and can effectively prevent wound infection and promote wound healing. In this study, in order to design wound dressing materials endowed with excellent antibacterial and tissue repair properties, we attempted to load antimicrobial peptides onto dopmine-modified graphene oxide (PDA@GO) using lysozyme (ly) as a model drug. Then, functionalized GO was used to the surface modification of arginine-modified chitosan (CS-Arg) membrane. To evaluate the potential of the prepared nanocomposite membrane in wound dressing application, the surface morphology, hydrophilic, mechanical properties, antimicrobial activity, and cytocompatibility of the resulting nanocomposite membrane were analyzed. The results revealed that prepared nanocomposite membrane exhibited excellent hydrophilic, mechanical strength and antimicrobial activity, which can effectively promote cell growth and adhesion. In particular, using PDA@GO as drug carrier can effectively maintain the activity of antimicrobial peptides, and can maximize the antibacterial properties of the nanocomposite membrane. Finally, we used rat full-thickness wound models to observe wound healing, and the surface interactions between the prepared nanocomposite membrane and the wound. The results indicated that nanocomposite membrane can obviously accelerated wound closure, and the wounds showed reduced inflammation, improved angiogenesis and accelerated re-epithelialization. Therefore, incorporation of antimicrobial peptides-functionalize graphene oxide (ly-PDA@GO) into CS-Arg membrane was a viable strategy for fabricating excellent wound dressing. Together, this study not only prepared a wound dressing with excellent tissue repair ability, but also provided a novel idea for the development of graphene oxide-based antibacterial dressing.
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高影响因子
PHYTOMEDICINE
·
2025
·
IF 8.3
Fan-Hao Wei,Wen-Yin Xie,Pei-Sen Zhao,Zhong-Hao Ji,Fei Gao,Cheng-Zhen Chen,Zhe Zhang,Wei Gao,Bao Yuan
Background The development of effective and safe dietary supplements is essential for both the prevention and management of ulcerative colitis (UC), as its pathogenesis is intricate and difficult to completely resolve. Crataegus pinnatifida, a medicinal food with a long history of use, has broad medicinal value. Recent research has revealed promising insights into the role of polysaccharide derived from Crataegus pinnatifida on modulating short-chain fatty acids (SCFAs) to alleviate UC inflammation. However, the mechanisms by which CPP regulates the intestinal microbiota and key metabolites during the antagonistic phase of UC have yet to be elucidated. Objective This research elucidated the protective role of CPP in relation to UC, highlighted the mechanisms through which CPP operates, particularly regarding gut microbiota and metabolism, and offered a theoretical foundation for the potential use of CPP as a dietary supplement aimed at preventing UC. Methods The impact of CPP on acute UC induced by 3% DSS in mice was examined through the evaluation of the disease activity index, measurement of colon length, and observation of body weight changes. Enzyme-linked immunosorbent assay (ELISA) was used to measure inflammatory factor levels in both serum and colon, as well as to assess oxidative stress mediators. The intestinal histological damage, mucus layer damage and the level of tight junction protein were analyzed by histopathological staining and western blot (WB). The impact of gut microbiota on CPP in colitis was evaluated using 16S rRNA sequencing, microbiota depletion experiments, and fecal microbiota transplantation (FMT) studies. The key metabolic pathways and key metabolites affected by CPP in the treatment of UC were analyzed through untargeted metabolomics sequencing, ELISA, and WB assays. Results Prophylactic dietary supplementation with Crataegus pinnatifida polysaccharide (CPP) notably reduced thenotably reduced the fundamental clinical manifestations of UC induced by DSS, including DAI score, reduced colon length, and weight loss, as well as inflammation and oxidative stress. CPP promoted the expression of Caludin-1, ZO-1 and Occludin and promoted mucin secretion, which contributed to the mitigation of intestinal barrier damage caused by DSS. 16S sequencing results and metabolomics results revealed that CPP intervention upregulated the relative abundance of Lactobacillus, thereby reshaping the intestinal microbiota and activate the arginine biosynthesis pathway. The results of fecal microbiota transplantation and antibiotic clearance experiments indicated that the alleviating effect of CPP on UC was dependent on the intestinal flora and that this alleviating effect was transferred through fecal microbiota transplantation. Mechanistically, CPP indirectly promoted the expression of the rate-limiting enzyme argininosuccinate synthase (ASS1) in the intestinal Arginine biosynthesis pathway by reshaping the intestinal microbiota, thereby increasing intestinal Arginine level and alleviating the inflammatory response and oxidative stress induced by DSS and intestinal barrier damage. Conclusion Our research findings demonstrate that CPP is a plant-derived polysaccharide that alleviates UC by modulating the gut microbiota and enhancing arginine biosynthesis.
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高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2020
·Vol. 56
·期 2
·999-1012
·
IF 2.773
Xiuyun Guo,Yawei Zhang,Muneer Ahmed Jamali,Zengqi Peng
Summary The present work examined the impact of L-Arginine (Arg) on the emulsifying properties, interfacial behaviour and conformational characteristics of myofibrillar proteins (MPs) at high (0.6 m ) and low (0.15 m ) salt concentration to maintain good emulsifying properties of MPs at low salt concentration. The data indicated that Arg increased the emulsifying activity index/emulsion stability index (EAI/ESI) and decreased the CI and droplet size of emulsions regardless of salt concentration. Raman spectra revealed that the α-helix content decreased from 60.30% to 51.26% at high salt concentration, and from 60.20% to 54.82% at low salt concentration in the presence of Arg. In addition, MPs treated with Arg exhibited a higher interfacial pressure and more rapidly diffusion to the oil surface. Meanwhile, Arg increased the interfacial protein loading. The results demonstrated that Arg caused the unfolding of MPs, promoting the adsorption of proteins and decreasing the interfacial tension, ultimately improving the stability of emulsions at low salt concentration.
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高影响因子
ADVANCED POWDER TECHNOLOGY
·
2019
·
IF 3.25
Yuting Chu,Muhammad Asim Khan,Fengyun Wang,Mingzhu Xia,Wu Lei,Sidi Zhu
Arg-Mt, was fabricated by modifying sodium montmorillonite (Na-Mt) with Arginine monohydrochloride (Arg salt), to adsorb Pb(II) and Cu(II) in aqueous solution. The X-ray powder diffraction (XRD), Fourier transform infrared (FTIR) spectrum, thermal analysis (TG/DTG), Brunauer-Emmett-Teller (BET) and Scanning electron microscope (SEM) were employed to investigate the properties of Na-Mt and Arg-Mt. The effects of the amount of modifier, pH value, the initial concentration of M(II), temperature and contact time were tested in a single adsorption system. The isotherm model was well matched with the Langmuir curve and the kinetic adsorption fitted well with the pseudo-second-order rate equation. The saturated adsorption of Pb(II) and Cu(II) by Arg-Mt were 124.69 and 29.15 mg/g, respectively, which were higher than that of Na-Mt (89.08 and 23.93 mg/g). The thermodynamic equation indicated that the adsorption process was spontaneous, endothermic, and the randomness of the samples changed a little after adsorption. The adsorption capacity of heavy metal ions in the binary co-adsorption system was poor than single adsorption system. Furthermore, the adsorbed M(II) onto Arg-Mt can hardly be dissolved under weak acidic condition (pH > 4) according to the desorption experiments. High-efficiency and low-cost make Arg-Mt to be used in the removal of heavy ions in aqueous solution.
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高影响因子
CyTA-Journal of Food
·
2019
·
IF 1.605
Tong Shi,Li Yuan,Jianlou Mu,Ruichang Gao
Amino acids containing L-Arginine (Arg), L-Lysine (Lys) and L-Histidine (His) are recognized to increase the solubility of myosin at low ionic strength, but there is a difference in the researchers’ views on their effects on the secondary structure of myosin. To elucidate which method is relatively appropriate for the determination of secondary structures of myosin added with Arg, Lys or His, Circular dichroism (CD) spectra and Raman spectroscopy were carried out respectively. Raman spectroscopy was more suitable than CD method for determination of the secondary structures of myosin added with His, while CD spectra was more accurate for the myosin samples added with Lys and the adequate concentration of Lys should be under 10 mM. Both CD and Raman methods could be applied to myosin added with Arg, however, the latter was recommended and the adequate additive content of Arg in CD method should be less than 10 mM.
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高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 12.5
Xin Shi,Qiuyan Bi,Zizhen Peng,Zhicheng Wang,Fangbo Zhu,Yakai Lin,Wenzhong Ma,Chuanjie Fang,Shiai Xu
RIP microenvironment regulation and amino acid functionalization synergize NF design. • BDM scavenges H + to suppress PEI protonation and preserve reactivity. • L-Arginine functionalization enhances membrane hydration and positive charge. • P-B-0.8/A-0.1 membrane achieves 99.5% Mg 2+ rejection and an SF of 131.7. • After 80 h, MgCl 2 rejection >98%, showing stable and antifouling performance. Thin-film composite (TFC) nanofiltration (NF) membranes show great potential for Mg 2+ /Li + separation. However, during conventional interfacial polymerization, excessively rapid amine diffusion and local acidification at the reaction interface can lead to nonuniform interfacial reactions. This study proposes a synergistic construction strategy of membrane forming microenvironment regulation and amino acid interface empowerment. Bis (2-dimethylaminoethyl) ether moderated the apparent migration and protonation behavior of polyethylenimine and alleviated local acidification at the reaction front. Subsequent L-arginine post modification enhanced surface hydration and modified the acid–base/charge characteristics of the polyamide layer interface. P-B-0.8/A-0.1 membrane with high positive surface charge (IEP pH = 8.5), excellent antifouling properties, and a uniform pore-size distribution were constructed for Mg 2+ /Li + separation. In the MgCl 2 /LiCl mixed salt system, the membrane achieved an Mg 2+ rejection rate of 99.5% and a separation factor of 131.7. During 80 h of continuous operation, the Mg 2+ rejection rate remained above 98%. Moreover, the P-B-0.8/A-0.1 membrane exhibited excellent flux recovery and anti-scaling stability during BSA and lysozyme fouling cycles, as well as in CaSO 4 and CaCO 3 inorganic scaling systems.
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高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Jun Qi,Yu-cong Li,Hui-min Yan,Li-ting Zeng,Ao-jing Lv,Rui-ling Dong,Xue-qing Li,Zhou Lin,Le-le Shao,Lang Zhang,Guo-qing Duan,Chun-hui Zhang,Guo-yuan Xiong
The impact of low-energy stirring temperatures (30 °C, 50 °C, 70 °C, 90 °C) on the stability of gelatin emulsions was investigated when stirring with a magnetic stirrer at 1600 rpm for 3.5 h at an arginine (Arg) concentration of 1.2 % (w/v). The results indicated that as the stirring temperature increased, the appearance of the emulsions became more transparent and clearer, and their particle sizes and the absolute zeta potential values decreased significantly ( P < 0.05). Moreover, the addition of Arg significantly enhanced the stability of the emulsions, with those prepared at 30 °C exhibiting the highest stability. These findings could be attributed to the fact that under the stirring temperature of 30 °C and in the presence of Arg, the depolymerization of gelatin increased, leading to a significant increase in the hydrophobic β-sheet structures ( P < 0.05), which in turn resulted in a significant increase in the exposure of hydrophobic amino acids and negatively charged residues on the gelatin surface ( P < 0.05). Ultimately, the stability of gelatin emulsions (φ = 5 %), prepared via this one-step stirring method, was significantly improved. The information gathered could provide guidance on preparing protein-stabilized emulsions with low oil phase volume fractions using low-energy emulsification techniques.
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.7
Yaxuan Zhao,Xuan Zhang,Min Sun,Ni An,Yuhuan Chen,Fang Yang
The poor water solubility of anticancer drugs significantly limits their therapeutic efficacy. Amphiphilic micelles with hydrophilic shells have emerged as promising anticancer drug carriers due to their remarkable ability to improve drug bioavailability. In this study, a Lysine-Arginine polyesteramide containing pendant carboxyl groups was synthesized (Lys-Arg-PEA). Subsequently, a series of amphiphilic comb-like copolymer micelle (AcDex-Lys-Arg-PEA) was developed through combination with acetylated dextran derivatives of different substitution degrees. The results revealed that the blank micelles maintained stable spherical structures with a uniform size distribution. The micellar system exhibited favorable hemocompatibility (hemolysis rate <5% and protein adsorption <30%), negligible cytotoxicity (cell viability >90% at 80 μg/mL concentration) and enzymatic degradability. Curcumin (Cur) was employed as a model drug to evaluate the anticancer drug delivery potential of this system. The micelles achieved high encapsulation efficiency (63%–85%) and drug loading capacity. Cur-loaded micelles showed significant inhibitory effects on HeLa cells (cell viability <55% at 80 μg/mL concentration). Drug release simulations in PBS buffer revealed pH-responsive characteristics, and the micelles exhibited faster Cur release at a cancerous pH (5.4, 50%–60%) than at a physiological pH (7.4, 30%–40%). This study demonstrates the potential of the comb-like copolymer micelles as effective carriers for anticancer drug delivery, providing valuable insights for subsequent research.
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高影响因子
Journal of Materials Chemistry B
·
2021
·Vol. 10
·期 4
·607-624
·
IF 6.331
Andy Wijaya,Yi Wang,Dan Tang,Yuan Zhong,Boyan Liu,Meng Yan,Quhui Jiu,Wei Wu,Guixue Wang
Nitric oxide (NO) is an exceptional endogenous biological gas that mediates and regulates physiological and pathological processes in the human body. However, its synthesis process is impaired during athero-progression and formation. Hence, a strategy to boost NO production and target endothelial nitric oxide synthase (eNOS) is crucial and intriguing in atherosclerosis (AS) management. Herein, we prepare L-arginine (LA) and lovastatin (LV) co-loaded PLGA nanomedicine to achieve sustainable release for enhancing NO production. The utilization of LA reveals that LA has dual contributions, acting as a NO donor and enhancing the solubility of LV by stabilizing PLGA NPs. PLGA-LA/LV demonstrated its potential to boost NO in vitro and in vivo confirmed using DAF-FM DA, augment eNOS and p-eNOS mRNA and protein levels, and suppress the ki67 proliferation marker in VSMCs; in addition, it lowers the total cholesterol level of blood plasma in C57BL/6 mice. Moreover, PLGA can protect the compound delivered and enhance the bioavailability to reach and get released in the blood circulation after oral administration. Collectively, our results endow a safe and efficient nanomedicine outcome, specifically with potential for AS management.
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