Bioresources and Bioprocessing
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2022
·Vol. 9
·期 1
·1-14
·
IF 4.983
Wang Jin,He Wubo,Tan Wen-Song,Cai Haibo
Epigallocatechin-3-gallate (EGCG) is a plant-derived flavonoid compound with the ability to promote the differentiation of human bone marrow-derived mesenchymal stem cells (MSCs) into osteoblasts. However, the effect of EGCG on the osteogenic differentiation of the human umbilical cord-derived mesenchymal stem cells (HUMSCs) is rarely studied. Therefore, in this study, the osteogenic effects of EGCG are studied in the HUMSCs by detecting cell proliferation, alkaline phosphatase (ALP) activity, calcium deposition and the expression of relevant osteogenic markers. The results showed that EGCG can promote the proliferation and osteogenic differentiation of the HUMSCs in vitro at a concentration of 2.5–5.0 μM. Unfortunately, the EGCG is easily metabolized by cells during cell culture, which reduces its bioavailability. Therefore, in this paper, EGCG-loaded microspheres (ECM) were prepared and embedded in chitosan/carboxymethyl cellulose/montmorillonite (CS/CMC/MMT) scaffolds to form CS/CMC/MMT-ECM scaffolds for improving the bioavailability of EGCG. The HUMSCs were cultured on CS/CMC/MMT-ECM scaffolds to induce osteogenic differentiation. The results showed that the CS/CMC/MMT-ECM scaffold continuously released EGCG for up to 22 days. In addition, CS/CMC/MMT-ECM scaffolds can promote osteoblast differentiation. Taken together, the present study suggested that entrainment of ECM into CS/CMC/MMT scaffolds was a prospective scheme for promotion osteogenic differentiation of the HUMSCs. Graphical Abstract
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Shourui Liu,Ruibao Ju,Zhenguo Zhang,Zhen Jiang,Jingzhao Cui,Wanshun Liu,Baoqin Han,Shuo Wang
Endoscopic submucosal dissection (ESD) is an effective treatment for polyps and early gastrointestinal cancers, but requires a high level of operator skill. Injecting submucosal materials (SIM) helps create a fluid cushion between the mucosal and muscular layers, making the procedure easier and reducing associated risks. However, SIMs commonly used in current clinical practice tend to spread quickly and fail to provide long-lasting submucosal fluid cushions (SFC). Thus, there is a critical need for a material that is easy to inject while also maintaining a durable barrier. We prepared succinylated hydroxybutyl chitosan (HBC-SA) by adding succinic anhydride (SA) to hydroxybutyl chitosan (HBC). The hydrogel had excellent temperature-sensitive properties and was able to be injected via an endoscopic injection needle even after gel formation. In vitro and in vivo studies showed that it has satisfactory biocompatibility. Functional experiments showed that the submucosal lifting properties of this hydrogel were significantly better than that of normal saline (NS) and sodium hyaluronate (SH), two commonly used clinical materials. In addition, the hydrogel possessed excellent hemostatic properties. Based on these results, HBC-SA is a promising candidate for submucosal injection during ESD.
研究领域:
胃肠病学
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生物医学工程
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材料科学
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Biomedical Materials
·
2024
·Vol. 19
·期 5
·055024
·
IF 3.9
Zhan Zhang,Xinzhe Zhao,Ziyu Song,Lu Wang,Jing Gao
The protein-polysaccharide nanofibers have attracted intensive attention in promoting wound healing, due to their components and nanoscale fibrous structure that mimics the native extracellular matrix (ECM). For the full-thickness wounds, in addition to promoting healing, hemostatic property and antibacterial activity are also of critical importance. However, currently, protein-polysaccharide-based nanofiber membranes exhibit poor mechanical properties, lack inherent hemostatic and antibacterial capabilities, as well as the ability to promote tissue repair. In this study, we developed composited membranes, which were composed of collagen (Col) and chitosan (Chs), through solvent alteration and post-processing, the membranes showed enhanced stability under physiological conditions, proper hydrophilic performance and improved mechanical property. Appropriated porosity and water vapor transmission rate, which benefit to wound healing, were detected among all the membranes except for Col membrane. Aimed at wound dressing, hemocompatibility, antibacterial activity and cell proliferation of the electrospun membranes were evaluated. The results indicated that the Col/Chs composited membranes exhibited superior blood clotting capacity, and the membranes with Chs exceeding 60% possessed sufficient antibacterial activity. Moreover, compared with Chs nanofibers, significant increase in cell grow was detected in Col/Chs (1:3) membrane. Taken together, the electrospun membrane with multiple properties favorable to wound healing, superior blood coagulation, sufficient antibacterial performance and promoting cell proliferation property make it favorable candidate for full-thickness skin wound healing.
研究领域:
生物医学工程
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材料科学
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再生医学
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Journal of Environmental Chemical Engineering
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2024
·
IF 7.4
Yutong Li,Xiaoxiao Ma,Yile Du,Shuangli Chen,Pingping Wu,Sijing Zhang
The immobilization of cadmium in soil to reduce its bioavailability is a common approach in soil remediation . However, fluctuations in soil pH, redox potential , and microbial activity can still lead to cadmium release. To address this challenge, MgFe 2 O 4 /chitosan carbon aerogel beads (MCSCA) were synthesized for in-situ remediation to remove Cd from the soil. A two-week remediation period was conducted to assess the effectiveness of MCSCA in Cd-contaminated soil. Different proportions (1%, 2%, and 3% by soil weight) of MCSCA were incorporated into the soil for remediation, followed by MCSCA separation through water flotation . The results demonstrated that after three remediation cycles, 55.46%, 72.27%, and 75.70% of the total cadmium were removed by 1%, 2%, and 3% MCSCA additions, respectively, corresponding to the removal of 76.51%, 87.39%, and 90.28% of bioavailable cadmium. Regeneration of MCSCA was accomplished using 0.1 M HCl , achieving cadmium desorption rates of 97.14%, 96.43%, and 90.48% for 1%, 2%, and 3% MCSCA additions, respectively. The regenerated MCSCA maintained higher remediation efficiency, removing 62.72%, 75.20%, and 80.96% of bioavailable cadmium even after three cycles, with MCSCA recovery rates of 94.40%, 92.30%, and 91.40%, respectively. In a pot experiment, pakchoi plants grown in soil post three rounds of remediation with different ratios (1%, 2%, and 3%) of MCSCA xhibited reductions in total cadmium content by 44.75%, 59.38%, and 69.88%, respectively, compared to plants grown in Cd-contaminated soil. In summary, this study underscores the effectiveness of MCSCA in remediating Cd-contaminated soils, offering a promising approach for soil remediation.
研究领域:
环境科学
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土壤学
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2024
·Vol. 25
·期 4
·2418
·
IF 4.9
Runing Liang,Dan Zhang,Junwei Guo,Shaohuang Bian,Cheng Yang,Lusi A,Weiwei Zhang,Feng Huang
Chitosan is widely used in the production of various hydrogels due to its non-biological toxicity, good biocompatibility, and strong biodegradability. However, chitosan-based hydrogels have not been widely used in tissue engineering due to their poor mechanical strength, poor stability and high biotoxicity of cross-linking agents. As a green technology, low temperature plasma is rich in active groups that can be involved in various chemical reactions, such as replacing the components on the chitosan chain, contributing to the cross-linking of chitosan. In this study, a plasma-assisted preparation method of chitosan-based hydrogels was developed and the properties, including mechanics, water absorption, and degradation (or stability), were characterized and analyzed. It is proved that plasma treatment plays a significant role in improving the mechanical strength and stability of hydrogels.
研究领域:
生物材料学
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组织工程学
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Zhicheng Liu,Hao Zhang,Huining Su,Yang Chen,Xunan Jing,Daquan Wang,Shaohui Li,Hao Guan,Lingjie Meng
Developing porous hemostatic sponges that are both biosafe and multifunctional remains a complex challenge. Conventional hemostatic techniques often fall short in managing bleeding effectively, leading to severe critical cases such as suboptimal hemostasis, increased infection risk, and complications arising from profuse bleeding. To address these deficits, our study introduces a novel multifunctional nanocomposite sponge that synergistically incorporates chitosan (CS), cellulose (Cel), graphene oxide (GO), and silver (Ag) nanoparticles. The resulting CS/Cel/GO/Ag developed demonstrates a swelling rate exceeding 3000 %, an absorption rate of over 2100 %, and the lowest stress surpassing 20 kPa at an initial 80 % strain. In vitro analyses reveal that the CS/Cel/GO/Ag sponge has excellent cytocompatibility, non-hemolytic nature, and competence in blood cell adherence and bacterial inhibition. In vivo evaluations further demonstrate that compared to conventional hemostatic methods, the sponge substantially enhances hemostatic efficacy, as evidenced by the marked reductions in clotting times and diminished blood loss compared to conventional hemostatic methods. Specifically, the test results of the CS/Cel/GO/Ag sponge across three different models are as follows: for the rat tail amputation model, the clotting time was 99 s, while blood loss was 222 mg; for the rat liver injury model, the clotting time was 129 s. while blood loss was 812 mg; for the rat femoral artery laceration model, the clotting time was 96 s, while blood loss was 758 mg. The compelling attributes of the CS/Cel/GO/Ag sponges position them as a promising solution for the acute management of bleeding. Their excellent performance indicates they have potential role to play in trauma care.
研究领域:
材料科学
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生物医学工程
·
止血
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纳米技术
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组织工程
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
·
IF 4.784
Yinfeng Tan,Hao Wu,Tianzhi Xie,Lingling Chen,Shuqian Hu,Huafeng Tian,Yutang Wang,Jianguo Wang
Some thermoduric food spoilage bacteria pose great threat to beverage industry . To tackle the challenge, hydroxypropyl trimethyl ammonium chloride chitosan (HTCC) grafted magnetic cellulose beads have been prepared via a dropping technology. Sodium periodate oxidation process was carried out to form dialdehyde functional groups on the regenerated cellulose beads mixed with maghemite nanoparticles . HTCC was anchored on the beads through Schiff base reaction. The structure and properties of HTCC anchored beads were evaluated by Fourier transform infrared (FTIR) spectra, field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM). Thermal stability of the beads was estimated by thermogravimetric analysis (TGA) and differential thermal gravity (DTG), and the decomposition temperature of the beads were around 200–300 °C. A long-term antibacterial activities of the beads against Alicyclobacillus acidoterrestris were confirmed caused by the covalent bond between HTCC and the beads. The biodegradable HTCC grafted cellulose beads may provide a novel approach for food safety management .
研究领域:
食品科学
·
材料科学
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2024
·
IF 5.7
Yan Lv,Jing Liu,Yujun Zhang,Yiyu Zhou,Jianying Huang,Wenjie Wang,Xiancheng Ye
Penicillium expansum is the major fungus that causes blue mold and produces patulin, threatening human health. Due to health and environmental pollution concerns, chitosan (CS) has attracted more and more attention as a safer alternative to synthetic fungicides for the control of blue mold. In the present study, four different benzimidazole groups were introduced onto CS by the acylation reaction to obtain benzimidazole-based chitosan derivatives (R1b–R4b). After being well-characterized with Fourier transform infrared spectroscopy (FTIR), ultraviolet–visible spectroscopy (UV–vis spectra), and nuclear magnetic resonance (NMR), their antifungal activities against P. expansum were screened. Results showed that the inhibitory effects of chitosan derivatives against the pathogen were significantly correlated with chitosan derivatives’ concentration and their structures. R4b was shown as optimum with good solubility and antifungal activity with a minimum inhibitory concentration (MIC) value of 0.5 mg/mL and a minimum fungicidal concentration (MFC) value of 2.0 mg/mL. The remarkable antifungal efficiency of R4b against P. expansum was further demonstrated in terms of spore germination, mycelial growth, patulin production, and the preliminary antifungal mechanism. R4b exhibited significant inhibition of patulin production, while its antifungal mechanism was revealed by destroying cell membrane integrity and inducing membrane depolarization. Furthermore, R4b treatment could significantly reduce the incidence of blue mold rot in apple fruit, and the MTT assay showed the nontoxicity of R4b against Raw 264.7, HBZY-1, and Caco-2 cells. Altogether, these results indicate that it is promising to be used as a fruit preservative in the future.
研究领域:
食品科学
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微生物学
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植物病理学
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2013
·Vol. 14
·期 12
·24305-24319
·
IF 2.464
Jian Chen,Gui-Dong Huang,Si-Rong Tan,Jiao Guo,Zheng-Quan Su
This study aimed to research the preparation and content determination of capsaicin-chitosan microspheres (CCMS) enteric coated tablets. The core tablets were prepared with the method of wet granulation. Nine formulae were designed to determine the optimal formula of the core tablet. Eudragit L100 was used to prepare the CCMS enteric-coated tablets. The effect of enteric coated formulation variables such as content of talc (10%, 25% and 40%), plasticisers (TEC and DBS), dosage of plasticiser (10%, 20% and 30%) and coating weight (2%, 3% and 5%) were evaluated for drug release characteristics. Thein vitrorelease was studied using 0.1 N HCl and pH 6.8 phosphate buffer. Enteric coated tablets without ruptures or swelling behaviour over 2 h in 0.1 N HCl indicated that these tablets showed acid resistance. The accumulated release rate in phosphate buffer (pH 6.8) revealed that the prepared tablets were able to sustain drug release into the intestine and a first-order release was obtained for capsaicin. This research is the first report of the preparation and content determination of CCMS enteric coated tablets. The sustained release behavior of enteric coated formulations in pH 6.8 phosphate buffer demonstrated that it would be a potential drug delivery platform for sustained delivery of gastric irritant drugs.
研究领域:
药剂学
·
药物递送系统
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高影响因子
PROCESS SAFETY AND ENVIRONMENTAL PROTECTION
·
2024
·
IF 6.9
Hao Liang,Nana Wang,Wei Tian,Hongping Zhang,Cuicui Li,Ruibin Guo,Zunli Mo,Nijuan Liu
Uranium extraction from unconventional uranium resources is among the major constraints to the development of the nuclear industry at present. In this dissertation, we here propose to effectively graft amidoxime groups on magnetic MXene and chitosan (CS) composites to extract uranium from low-enriched uranium solutions (MCF-AO). The data of adsorption experiments displayed that MCF-AO possessed fast adsorption kinetics (within 120 min), outstanding selectivity and salient adsorption capacity (1305.7 mg/g at pH=4, T=298.15 K) for uranium and could be easily separated with a magnet. Adsorption studies revealed a behavior consistent with the pseudo-second-order kinetic model and the Langmuir adsorption isotherm model, which is suitable for the spontaneous heat-absorption chemical monolayer adsorption process . Its sorption remains at more than 55 % of the original after 5 adsorption-desorption tests, and has great tolerance to interfering ions. Mechanistic research pointed out that the selective adsorption of uranium by MCF-AO was significantly enhanced by the presence of hydroxyl, amino and amidoxime groups. Meanwhile, MCF-AO exhibited superb resistance to two common bacteria (E. coli and B. subtilis ) and achieved more than 95 % uranium extraction capacity in simulated seawater , even more 4.12 mg/g of uranium extraction in Salt Lake water. Consequently, MCF-AO has a broad application prospect in the recovery and treatment of complex uranium systems.
研究领域:
材料科学
·
环境科学
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高影响因子
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
·
2013
·Vol. 13
·期 6
·3832-3840
·
IF 1.149
Cui Qinmin,Dai Lijun,Yang Liu,Ziener Ulrich,Cao Zhihai
Cross-linked chitosan (CS) nanohydrogels were successfully prepared via cross-linking CS nanodroplets in inverse miniemulsions. The cross-linker was transferred to the CS nanodroplets via the evaporation from its aqueous solution and diffusion through the continuous phase. The formation
of cross-linked CS nanohydrogels was confirmed by the morphological investigation during the reaction and the successful preparation of acidic aqueous dispersion of CS nanohydrogels. The size and size distribution of the CS nanodroplets and nanohydrogels were characterized by dynamic light
scattering (DLS). The particle morphology of CS nanohydrogels was observed by transmission electron microscopy (TEM). The influence of the synthetic parameters on the particle properties and colloidal stability was investigated with respect to sonication time, surfactant type and amount, type
of low polarity solvent, and concentration of CS solution. The cross-linked CS nanohydrogels could be easily re-dispersed in water, and showed a pH sensitivity.
研究领域:
材料科学
·
生物医学工程
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Current Drug Delivery
·
2022
·Vol. 19
·期 1
·32-40
·
IF 3.758
Guo Hejian,Yan Xuehui,Tang Hao,Zhang Xiaoyan
Background: Exenatide(EXE) is an anti-hyperglycemic agent approved for treating type 2 diabetes by the Food and Drug Administration(FDA). However, twice-daily injection of exenatide is inconvenient for most of the patients. Objective: In this study, biotinylated trimethylated chitosan(Bio-TMC)
based nanoparticles were proposed to promote oral absorption of exenatide. Realizing the oral administration of exenatide is very important to alleviate patient suffering and improve patient compliance. Methods: Bio-TMC was synthesized, and the chemical structure was characterized by
Fourier transform infrared (FT-IR) spectroscopy and 1H NMR spectroscopy. Nanoparticles were prepared through polyelectrolyte interaction in the presence of sodium Tripolyphosphate (TPP) and hydroxypropyl methylcellulose phthalate (HP-55). Formulations were physically and chemically characterized.
In vitro release was investigated in different pH media. In vivo antidiabetic activities of biotin modified and non-biotin modified chitosan were evaluated in db/db mice. Results: EXE-loaded Bio-TMC/HP-55 nanoparticles were spherical in shape with
a mean diameter of 156.2 nm and zeta potential of +11.3 mV. The drug loading efficiency and loading content were 52.38% and 2.08%, respectively. In vitro release revealed that EXE-loaded Bio-TMC/HP-55 nanoparticles were released faster in pH 1.2 than pH 6.8 (63.71% VS 50.12%),
indicating that nanoparticles have enteric characteristics. Antidiabetic activity study revealed that after oral administration to diabetic mice, the relative pharmacological bioavailability (FPharm%) of the biotin modified nanoparticles was found to be 1.27-fold higher
compared to the unmodified ones, and the hypoglycemic effect was also found to be better. Conclusion: Bio-TMC/HP-55 nanoparticles are feasible as oral drug carriers of exenatide and have the potential to be extended to other drugs that are not readily oral, such as monoclonal antibodies,
vaccines, genes, etc. These would be beneficial to the pharmaceutical industry. Further research will focus on the biodistribution of Bio-TMC/HP-55 nanoparticles after oral administration.
研究领域:
药剂学
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生物医学工程
·
药物递送系统
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高影响因子
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