JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2024
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IF 9.4
Akbar Mamatali,Dedong Wu,Haijiao Xie,Pengfei Xiao
Metronidazole (MNZ), a commonly used antibiotic, poses risks to water bodies and human health due to its potential carcinogenic, mutagenic, and genotoxic effects. In this study, mesoporous cobalt-manganese layered double hydroxides (Co x Mn y -LDH) with abundant oxygen vacancies (O v ) were successfully synthesized using the co-precipitation method and used to activate calcium sulfite (CaSO 3 ) with slight soluble in water for MNZ degradation. The characterization results revealed that Co 2 Mn-LDH had higher specific areas and exhibited good crystallinity. Co 2 Mn-LDH/CaSO 3 exhibited the best catalytic performance under optimal conditions, achieving a remarkable MNZ degradation efficiency of up to 98.1 % in only 8 min. Quenching experiments and electron paramagnetic resonance (EPR) tests showed that SO 4 •− and 1 O 2 played pivotal roles in the MNZ degradation process by activated CaSO 3 , while the redox cycles of Co 2+ /Co 3+ and Mn 3+ /Mn 4+ on the catalyst surface accelerated electron transfer, promoting radical generation. Three MNZ degradation routes were put forward based on the density functional theory (DFT) and liquid chromatography-mass spectrometer (LC-MS) analysis. Meanwhile, the toxicity analysis result demonstrated that the toxicity of intermediates post-catalytic reaction was decreased. Furthermore, the Co 2 Mn-LDH/CaSO 3 system displayed excellent stability, reusability, and anti-interference capability, and achieved a comparably high removal efficiency across various organic pollutant water bodies. This study provides valuable insights into the development and optimization of effective heterogeneous catalysts for treating antibiotic-contaminated wastewater.
研究领域:
环境科学
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催化化学
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水处理技术
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PubMed
高影响因子
AQUATIC TOXICOLOGY
·
2023
·
IF 4.5
Yu Han,Weixia Zhang,Yu Tang,Wei Shi,Zhiquan Liu,Imane Lamine,Hangjun Zhang,Jing Liu,Guangxu Liu
Omnipresent presence of triclosan (TCS) in aqueous environment puts a potential threat to organisms. However, it's poorly understood about its immunometabolic impacts of marine invertebrates. In present study, we use a representative bivalve blood clam ( Tegillarca granosa ) as a model, investigating the effects of TCS exposure at 20 and 200 μg/L for 28 days on immunometabolism, detoxification, and cellular homeostasis to explore feasible toxicity mechanisms. Results demonstrated that the clams exposed to TCS resulting in evident immunotoxic impacts on both cellular and humoral immune responses, through shifting metabolic pathways and substances, as well as suppressing the expressions of genes from the immune– and metabolism–related pathways. In addition, significant alterations in contents (or activity) of detoxification enzymes and the expression of key detoxification genes were detected in TCS-exposed clams. Moreover, exposure to TCS also disrupted cellular homeostasis of clams through increasing MDA contents and caspase activities, and promoting activation of the apoptosis–related genes. These findings suggested that TCS might induce immunotoxic impacts by disrupting the immunometabolism, detoxification, and cellular homeostasis.
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高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2024
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IF 6
Huimin Shao,Xiaoyun Su,Yaru Wang,Jie Zhang,Tao Tu,Xiaolu Wang,Huoqing Huang,Bin Yao,Huiying Luo,Xing Qin
Mycotoxins are widely present from cereal crops to food products, posing a significant risk to food safety and causing serious health and economic losses. Therefore, it is imperative to develop a sustainable and efficient detoxification strategy to address mycotoxin contamination. Herein, a dye-decolorizing peroxidase, Ba DyP, from the white-rot fungus Bjerkandera adusta was successfully expressed as a soluble form in Escherichia coli with the help of molecular chaperones. Purified recombinant Ba DyP could efficiently degrade multiple mycotoxins including aflatoxin B 1 , zearalenone, and deoxynivalenol in the presence of mediators such as Mn 2+ or 1-HBT. Meanwhile, their degradation products were identified using UPLC-MS/MS, including AFB 1 -diol, AFQ 1 , 15-OH-ZEN, HZEN, and C 15 H 18 O 8 . Furthermore, cell survival tests exhibited a significant reduction in the biological toxicity of these degradation products. These findings indicate that the use of dye-decolorizing peroxidase could be a promising approach for the effective removal of multiple mycotoxins in cereal crops and food products.
研究领域:
食品安全
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微生物学
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环境科学
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DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2024
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IF 4.9
Jianing Liu,Yi Huang,Ying Chen,Haobo Ke,Qian Deng,Hua Tang,Jinyan Yang,Shijun Ni
With industry development, water contaminated with vanadium(V) has attracted increasing attention. Various adsorbents have been used to remove V(V); however, reducing V(V) to V(III) or V(IV) with low solubility and toxicity remains a challenge. Herein, a novel layered double hydroxide loading with polyaniline composites (PANI-LDHs) adsorbent was prepared to facilitate V(V) detoxification and elimination. We examined the physicochemical characteristics and confirmed the successful formation of the composite. Some key experimental parameters were evaluated in detail for their potential impact on V(V) removal. The calculated maximum adsorption capacity was 222.22 mg/L with a dosage of only 0.3 g/L under 318 K, which was higher than other adsorbents. More importantly, about 80 % of the absorbed V(V) was reduced and detoxified by reductive nitrogen-containing groups in PANI. In addition, the PANI-LDHs can effectively resist the interference of various coexisting ions. The excellent V(V) removal performance is mainly attributed to the high stability of the inorganic skeleton (LDH) and the high chemical activity of PANI. Dynamics and thermodynamic analysis were also used to study the V(V) adsorption behavior. Finally, the mechanism study suggested that V(V) anions were adsorbed onto the surface of PANI-LDHs via electrostatic attraction or the H-bonding. The adsorbed V(V) was subsequently reduced and immobilized to V(IV) and V(III) oxides by nitrogen-containing groups in PANI, thus achieving the detoxification and removal of V(V). The combination of adsorption and detoxification properties, high adsorption capacity, and good selectivity make PANI-LDHs an ideal candidate for V(V)-containing wastewater treatment.
研究领域:
环境科学
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材料科学
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DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2024
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IF 12.2
Mingtao Tan,Hong Jiang,Rusong Chai,Miao Fan,Zengting Niu,Guotong Sun,Shanchun Yan,Dun Jiang
Heavy metal pollutants are important abiotic environmental factors affecting pest habitats. In this study, Cd pre-exposure significantly increased the tolerance of Lymantria dispar larvae to β-cypermethrin, but did not significantly alter their tolerance to λ-cyhalothrin and bifenthrin. The activation of P450 by Cd exposure is the key mechanism that induces insecticide cross-tolerance in L. dispar larvae. Both before and after β-cypermethrin treatment, Cd exposure significantly increased the expression of CYP6AB224 and CYP6AB226 in L. dispar larvae. Silencing CYP6AB224 and CYP6AB226 reduced the tolerance of Cd-treated L. dispar larvae to β-cypermethrin. Transgenic CYP6AB224 and CYP6AB226 genes significantly increased the tolerance of Drosophila and Sf 9 cells to β-cypermethrin, and the recombinant proteins of both genes could significantly metabolise β-cypermethrin. Cd exposure significantly increased the expression of CnCC and Maf . CnCC was found to be a key transcription factor regulating CYP6AB224 - and CYP6AB226 -activated insecticide cross-tolerance in Cd-treated larvae. Decreasing reactive oxygen species (ROS) levels in the Cd-treated larvae or increasing ROS levels in the untreated larvae reduced or enhanced the expression of CnCC , CYP6AB224 and CYP6AB226 and β-cypermethrin tolerance in L. dispar larvae, respectively. Collectively, Cd exposure confers β-cypermethrin tolerance in L. dispar larvae through the ROS/ CnCC signalling pathway-mediated P450 detoxification. Environmental Implication Heavy metals have been reported to produce cross-tolerance in agricultural insects to insecticides. The growing demand for chemical pest control necessitates expanding research on the widespread occurrence of cross-tolerance to insecticides among pests, particularly forest pests, in areas polluted with heavy metals. In this study, Cd pre-exposure enhanced the cross-tolerance of Lymantria dispar larvae to β-cypermethrin. Cd pre-exposure activated the P450 detoxification system of L. dispar larvae. CYP6AB224 and CYP6AB226 were key detoxify genes to the formation of cross-tolerance. ROS-CnCC signaling pathway was proved to be responsible for activation of detoxify genes.
研究领域:
环境科学
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昆虫学
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分子生物学
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毒理学
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PubMed
高影响因子
CHEMICO-BIOLOGICAL INTERACTIONS
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2025
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IF 5.4
Rong Tian,Jia-Xin Li,Naihao Lu
As a replacement of bisphenol A, bisphenol S (BPS) is commonly used in the wrappers and food containers of daily life. Epidemiological studies demonstrate a close link between BPS exposure and vascular diseases, where the biological activities of BPS remain scarcely known. Herein, the effects of BPS on endothelial function as well as the underlying mechanism were investigated in human umbilical vein endothelial cells (HUVECs) and mouse arteries. It was found that exposure of BPS dose-dependently induced endothelial dysfunction (i.e., decline of nitric oxide (NO) formation) in HUVECs, accompanied by the increase of reactive oxygen species (ROS) production and loss of mitochondria membrane potential. Mitochondria-specific antioxidant (Mito-Tempol) or superoxide scavenger (tiron) abolished the harmful effects of BPS, while superoxide dismutase (SOD)-specific siRNA exhibited negative influence, suggesting that mitochondrial ROS was responsible for BPS-induced endothelial dysfunction and SOD was a sensitive target of BPS. Consistently, plasma NO formation and endothelium-dependent vasodilation was significantly impaired in mice exposed to dietary BPS. In addition, the binding of bovine serum albumin (BSA, the most abundant protein in blood) to BPS considerably alleviated ROS formation and endothelial dysfunction in HUVECs. BPS primarily interacted with Sudlow site I of albumin to generate BSA-BPS complex through static mechanism, in which the hydrogen bonds and electrostatic forces played important roles. Altogether, dietary exposure to emerging BPS would disrupt vascular homeostasis via the induction of mitochondrial ROS formation and consequent endothelial dysfunction, highlighting the detoxification impact of albumin protein on the hazardous effects of environmental pollutants.
研究领域:
环境科学
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心血管生物学
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毒理学
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PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2024
·
IF 6.2
Shuxue Zhao,Xiaohang Li,Xingdong Yao,Xuyang Liu,Chao Pan,Lizhong Guo,Jie Bai,Tiantian Chen,Hao Yu,Chunhui Hu
Fungal laccase has strong ability in detoxification of many environmental contaminants. A putative laccase gene, LeLac12 , from Lentinula edodes was screened by secretome approach. LeLac12 was heterogeneously expressed and purified to characterize its enzymatic properties to evaluate its potential use in bioremediation. This study showed that the extracellular fungal laccase from L. edodes could effectively degrade tetracycline (TET) and the synthetic dye Acid Green 25 (AG). The growth inhibition of Escherichia coli and Bacillus subtilis by TET revealed that the antimicrobial activity was significantly reduced after treatment with the laccase-HBT system. 16 transformation products of TET were identified by UPLC-MS-TOF during the laccase-HBT oxidation process. Gas chromatography-mass spectrometry (GC-MS) analysis revealed that LeLac12 could completely mineralize ring-cleavage products. LeLac12 completely catalyzed 50 mg/L TET within 4 h by adding AG (200 mg/L), while the degradation of AG was above 96% even in the co-contamination system. Proteomic analysis revealed that central carbon metabolism, energy metabolism , and DNA replication/repair were affected by TET treatment and the latter system could contribute to the formation of multidrug-resistant strains. The results demonstrate that LeLac12 is an efficient and environmentally method for the removal of antibiotics and dyes in the complex polluted wastewater.
研究领域:
环境科学
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微生物学
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生物化学
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Tengfei Xiong,Mingyue Yu,Mei Li,Christopher A. Reilly,Xinghui Qiu
Capsaicin and dihydrocapsaicin are the principal pungent compounds in hot peppers. The generalist Helicoverpa armigera and the specialist H. assulta are two of the few insects that can feed on hot pepper fruits. To further understand the mechanisms behind physiological adaptation to capsaicinoids in the two agricultural pests, we investigated the transcriptional response and metabolism capacity of CYP6B6 orthologs from the two closely related species to the capsaicinoids. The results showed that the expression of CYP6B6 was either weakly or moderately induced by capsaicin in a tissue-specific and dose-dependent manner. CYP6B6 induction to greater extent was observed in the larval midguts of the cotton bollworms, indicating that inducible defense is more robust in the generalist. The two CYP6B6 enzymes were able to efficiently metabolize both capsaicin and dihydrocapsaicin, and HassCYP6B6 exhibit a slightly higher activity towards capsaicin than HarmCYP6B6, as evaluated by the specific activity and Vmax values. Multiple known and yet to be elucidated metabolites were detected in the reactions catalyzed by CYP6B6s, with dehydrogenation at the terminal carbon followed by N-macrocyclization and aliphatic hydroxylation being the dominant pathways. The data support the hypothesis that these two Helicoverpa species have well adapted to capsaicinoids via their high P450-mediated detoxification ability.
研究领域:
昆虫学
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分子生物学
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植物与昆虫互作
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PubMed
高影响因子
npj Clean Water
·
2025
·
IF 11.4
Jung Sung-Hyo,Hong Sung-Hak,Lee Youn-Jun,Park Seong-Jik,Jho Eun Hea,Lee Chang-Gu
Azo is a synthetic organic dye that has attracted considerable attention because of its recalcitrance to degradation and toxicity. An upgraded ozonation process must be developed that integrates decolorization, mineralization, and toxicity reduction to manage the residual azo dye in the effluent from the dyeing industry and reduce its associated aquatic environmental risks. In this study, an ozonation system using Meretrix lusoria (ML) shell-waste-derived biomass (ML800) that uses simple calcination was developed. The ML800/O 3 system almost completely decolorized ( > 99.0%) and highly mineralized (53.6 ± 1.7%) Congo red (CR) during (CR = 100 mg∙L –1 , ML800 = 0.5 g ∙ L –1 ), surpassing the performance of the other tested systems (single ozonation and single ML800). Moreover, the ML800/O 3 system reduced the acute toxicity of CR to the bacterium Aliivibrio fischeri , whereas single ozonation showed temporarily increased the toxicity of CR. The FE-SEM/EDS, FTIR, and XRD analyses verified that Ca(OH) 2 was the main calcium species in ML800, which catalyzed the decomposition of O 3 into highly reactive •OH. The system was successfully applied to various azo dyes and was robust with water matrix constituents. These findings highlight the potential of marine shell waste for use as a sustainable and ecofriendly additive for ozonation, increasing azo dye removal from wastewater in practical applications.
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高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2024
·
IF 9.4
Jianchao Liu,Yihao Qin,Guanghua Lu,Runren Jiang
Photocatalysts are one of the effective methods to degrade antibiotic contamination, but the efficiency is low and the toxicity is not well recognized. Deep lattice doping to induce defect generation is an effective way to improve the performance of photocatalysts. Here, defect-rich bromine-doped BiOCl-XBr photocatalysts were constructed with the help of small molecules inserted into the interlayer. The photocatalytic degradation performance of BiOCl-XBr was significantly enhanced, and its degradation rate was up to about 12 times that of BiOCl monomer. The main reasons for the stronger photocatalytic performance of BiOCl-XBr include Br doping to enhance visible light absorption, surface defects, and Bi valence changes to improve charge transport. The degradation of tetracycline (TC) produced more toxic intermediates, and the biotoxicity experiments also confirmed that the toxicity showed a trend of increasing and then decreasing, indicating that the more toxic intermediates were also mineralized during the degradation process. However, the mortality and hatching rate of zebrafish in the exposed group after degradation recovered but changed their activity pattern under light and dark conditions. This further warns us to focus on the toxicity changes after antibiotic degradation. Finally, based on the free radical analysis, the mechanism of photocatalytic degradation and detoxification of TC by BiOCl-XBr was proposed.
研究领域:
环境科学
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材料科学
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生物学
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PubMed
高影响因子
PLoS Pathogens
·
2024
·Vol. 20
·期 7
·e1012431
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IF 5.5
Xiaoyu Liu,Nemat O. Keyhani,Hong Liu,Yue Zhang,Yuxian Xia,Yueqing Cao
Reactive carbonyl and oxygen species (RCS/ROS), often generated as metabolic byproducts, particularly under conditions of pathology, can cause direct damage to proteins, lipids, and nucleic acids. Glyoxal oxidases (Gloxs) oxidize aldehydes to carboxylic acids, generating hydrogen peroxide (H 2 O 2 ). Although best characterized for their roles in lignin degradation, Glox in plant fungal pathogens are known to contribute to virulence, however, the mechanism underlying such effects are unclear. Here, we show that Glox in the insect pathogenic fungus, Metarhizium acridum , is highly expressed in mycelia and during formation of infection structures (appressoria), with the enzyme localizing to the cell membrane. MaGlox targeted gene disruption mutants showed RCS and ROS accumulation, resulting in cell toxicity, induction of apoptosis and increased autophagy, inhibiting normal fungal growth and development. The ability of the MaGlox mutant to scavenge RCS was significantly reduced, and the mutant exhibited increased susceptibility to aldehydes, oxidative and cell wall perturbing agents but not toward osmotic stress, with altered cell wall contents. The Δ MaGlox mutant was impaired in its ability to penetrate the host cuticle and evade host immune defense resulting in attenuated pathogenicity. Overexpression of MaGlox promoted fungal growth and conidial germination, increased tolerance to H 2 O 2 , but had little to other phenotypic effects. Transcriptomic analyses revealed downregulation of genes related to cell wall synthesis, conidiation, stress tolerance, and host cuticle penetration in the Δ MaGlox mutant. These findings demonstrate that MaGlox -mediated scavenging of RCS is required for virulence, and contributes to normal fungal growth and development, stress resistance.
The ability of organisms to detoxify reactive metabolic byproducts is crucial for normal development, stress resistance, and where applicable, pathogenesis. Here, using the insect pathogenic fungus, Metarhizium acridum , we show that a glyoxal oxidase acts to detoxify cellular reactive carbonyl species (RCS) as well as leading to decreased reactive oxygen species (ROS) levels by alleviating aldehydes accumulation which can trigger ROS production. Mutants deficient in MaGlox showed increased levels of RCS/ROS leading to increased autophagy and apoptosis, resulting in impaired fungal growth, development, and reduced virulence. These findings link Glox mediated RCS/ROS detoxification to cell degradation/turnover processes and cell death, impacting fungal pathogenicity and development.
研究领域:
微生物学
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真菌学
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病理学
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细胞生物学
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植物病理学
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PubMed
高影响因子
WATER RESEARCH
·
2024
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IF 11.4
Yu-Jia Nong,Qian-Yuan Wu,Yun-Peng Wu,Ju-Won Lee,Min-Yong Lee,Wen-Long Wang
The KrCl-excimer lamp, emitting far-UVC light at 222 nm (UV222), offers a promising alternative to conventional UVC light at 254 nm (UV254) for the photolysis of organic pollutants and the activation of radical sensitizers. This study was aimed to investigate the efficiencies of UV222 in the treatment of halogenated aromatics, focusing on its performance in degradation, dechlorination and detoxification. Chlorophenols, representative recalcitrant and toxic halogenated aromatics, were used as target pollutants. The pathways of direct photolysis, photooxidation and photoreduction under UV222 illumination were compared. UV222 outperformed UV254 in photolyzing chlorophenols (1.4−34.1 times faster), especially protonated chlorophenols, due to substantially higher UV absorption (17.1−108.0 times) and quantum yields (2.1−3.4 times). The quantum yields of chlorophenols were influenced by the inducive electron-withdrawing effect of Cl-substitutes. Moreover, UV222 improved the dechlorination of chlorophenols to 95 % compared to 60 % by UV254. The introduction of radical sensitizer (e.g., H 2 O 2 , nitrate, and sulfite) reduced 4-chlorophenol photolysis by competing for UV222 absorption, though the sensitizers partially increased radical oxidation via generating • OH or e aq − . UV222 photolysis of 4-chlorophenol increased the toxicity by 88.6 times through forming toxic intermediates (e.g., hydroquinone and resorcinol). Notably, • OH and e aq − (i.e., UV222/H 2 O 2 and UV222/sulfite) increased the dechlorination and • OH (i.e., UV222/H 2 O 2 ) detoxified the mixture solution. Moreover, UV222 photolysis remained effective for 4-chlorophenol removal in real paper-mill wastewater, indicating the potential application of KrCl* lamp UV222.
研究领域:
环境科学
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化学工程
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PubMed
高影响因子
危险品化学品经营许可证（带存储）