POLYMER
·
2015
·
IF 3.562
Chun-Yu Zhang,Jing-Hui He,Cai-Jian Lu,Qian-Feng Gu,Lin-Xin Wu,Quan Liu,Hua Li,Qing-Feng Xu,Jian-Mei Lu
Two polymer-Hemin composites, AB type block copolymer (PStCH- b -P4VP): Hemin and homopolymer of poly(4-vinylpyridine) (P4VP): Hemin, can be fabricated into sandwich memory devices. The memory device characteristics of the PStCH- b -P4VP: Hemin blends can show Flash memory behavior, while the P4VP polymer with Hemin only shows write-once-read-many-times (WORM) performance. Charge transfer and nanocomposite redox are probably responsible for the conductance-switching behavior. The results of cyclic voltammogram (CV) measurement indicate that the reversible Fe III /Fe II redox reaction is preserved well in the composite PStCH- b -P4VP: Hemin, while only polymer oxidation is observed in the P4VP: Hemin system. Due to the hindrance of StCH moiety, the coordination of pyridine and Fe II can keep the Fe II active. The P4VP has a more flexible chain that saturates the Fe II center and results in stable coordination; Fe III /Fe II redox reaction is therefore an obstacle. By tuning the coordination surroundings of the metal ion , different memory types can be obtained.
研究领域:
材料科学
·
化学
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高影响因子
Analytical Methods
·
2015
·Vol. 7
·期 21
·9340-9346
·
IF 1.821
Kewei Wang,Na Sun,Xinpan Li,Ruihua Zhang,Guangyue Zu,Jine Wang,Renjun Pei
This study reported the development of a p–n heterojunction photoelectrochemical biosensor using a G-quadruplex as the recognition system. The n-type ZnO nanorods and p-type BiOI nanoflakes were combined to form the heterostructure by in situ growth of BiOI attributed to a simple impregnating-hydroxylation method. This simple p–n heterojunction fabrication method could facilitate the development of rapid and sensitive visible light activated PEC sensors. In this study, the ZnO/BiOI photoelectrode showed a rapid photocurrent decrease in the presence of Pb2+ due to the electron withdrawing effect of hemin which bound with the G-quadruplex/Pb2+ complex. This PEC biosensor exhibited a good response following the concentration of Pb2+ in the range from 10 to 100 μM.
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高影响因子
BIOSENSORS & BIOELECTRONICS
·
2014
·
IF 6.451
Zhenting Yang,Jing Qian,Xingwang Yang,Ding Jiang,Xiaojiao Du,Kan Wang,Hanping Mao,Kun Wang
A facile aptasensor has been developed for the colorimetric detection of acetamiprid by using the hemin-functionalized reduced graphene oxide (hemin-rGO) composites. The as-prepared hemin-rGO composites possessed both the ability of rGO to physically adsorb the aptamers and the peroxidase-like activity of hemin that could catalyse 3,3,5,5-tetramethylbenzidine (TMB) in the presence of H 2 O 2 , to produce a solution with blue color. The well-dispersed hemin-rGO composites coagulated completely at the proper salt concentration ; however, the coagulation of hemin-rGO was vanished when abundant aptamers were adsorbed on its surface because the attached negatively charged DNA backbone increased individual hemin-rGO electrostatic repulsion . In the detection scheme, acetamiprid with different concentrations was firstly incubated with the same amount of aptamer. The more acetamiprid in the tested solution, the less free aptamers were absorbed on the hemin-rGO surface, making the composites coagulate to a higher degree in the presence of the optimum NaCl concentration. As a consequence, the content of hemin-rGO in the supernatant was decreased after centrifugation, which catalysed oxidation of TMB in the presence of H 2 O 2 to produce light blue color with a low absorbance. The color variation relavant to the acetamiprid concentration can be judged by the naked eyes and easily monitored by the inexpensive UV–vis spectrometer. Such designed aptasensor displayed a linear response for acetamiprid in the range from 100 nM to 10 μM with a detection limit of 40 nM ( S / N =3). This colorimetric aptasensing platform offers great advantages including the simple operation process, low-cost portable instrument, and user-friendly applications.
研究领域:
分析化学
·
纳米技术
·
环境科学
·
传感器
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DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2017
·
IF 7.78
Shuai Chen,Pin Liu,Kewen Su,Xuan Li,Zhen Qin,Wei Xu,Jun Chen,Chaorui Li,Jingfu Qiu
In this work, novel octahedral Cu 2 O-Au nanocomposites were synthesized and first applied in an electrochemical aptasensor to detect thrombin (TB) with the aid of a DNAzyme for signal amplification . The octahedral Cu 2 O-Au nanocomposites have not only simultaneously served as signal amplifying molecules but have also been utilized as an ideal loading platform to immobilize a large number of electroactive substances and recognition probes. Gold nanoparticles (AuNPs) were grown directly on the surface of the octahedral Cu 2 O nanocrystals , and the Cu 2 O-Au nanocomposites obtained had the advantages of large surface areas and excellent biocompatibilities. The hemin/G-quadruplex, which was formed by intercalating hemin into the amino terminated thrombin binding aptamer (NH 2 -TBA), and the electroactive toluidine blue (Tb) were immobilized onto the Cu 2 O-Au nanocomposite surfaces through a stable Au-N bond. AuNPs, Cu 2 O and hemin/G-quadruplex co-catalyse the H 2 O 2 in the working buffer to promote the electron transfer of Tb as a multiple signal amplification strategy in order to improve the performance of the electrochemical aptasensor. Under optimal conditions, the designed aptasensor exhibited sensitive detection of TB from 100 fM to 20 nM with a lower detection limit of 23fM. This proposed aptasensor exhibited good sensitivity, high specificity and acceptable reproducibility and could be widely applied in bioassay analysis.
研究领域:
生物化学
·
纳米医学
·
分析化学
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DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2016
·
IF 7.476
Fengping Liu,Jiaqian Tang,Jun Xu,Yun Shu,Qin Xu,Hongmei Wang,Xiaoya Hu
An amperometric sensor was firstly established for the detection of indole-3-acetic acid (IAA) at low potential based on the hemin/reduced graphene oxide (hemin/rGO) composite. The hemin/rGO nanocomposite was prepared by a simple and facile hydrothermal method without using any reducing agent. It exhibited peroxidase-like activity for the catalytic oxidation of IAA in the presence of oxygen. The consumption of oxygen has a linear relationship with the concentration of IAA in the range from 0.1 to 43 μM and from 43 to 183 μM. The detection limit was down to 0.074 μM. This sensor was unaffected by many interfering substances and stable over time. Such work broadened the application of hemin/rGO and provided a new method for IAA detection.
研究领域:
植物学
·
分析化学
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DOI
PubMed
高影响因子
Analytical Methods
·
2017
·Vol. 10
·期 1
·84-90
·
IF 1.9
Xia Lin,Shu Zhu,Qiao Xia,Jiao Ma,Yingzi Fu
Herein, an ultrasensitive and stereo-selective electrochemiluminescent (ECL) biosensor based on ECL signal amplification of luminol by the synergetic catalysis of a hemin-functionalized composite and gold–platinum nanowires (Au-PtNWs) has been designed for the detection of D-alanine (D-Ala). In the sensor design, D-amino acid oxidase (DAAO) was used to generate the ECL co-reactant (H2O2) for luminol in situ. The reduced graphene oxide-hemin-cysteine (rGO-H-Cys) composite and Au-PtNWs acted not only as amplification labels to amplify signals via synergetic catalysis, but also as an ideal nanocarrier to accelerate electron transfer. A great difference in ECL intensities toward D-Ala and L-Ala was obtained, and an obviously higher intensity was seen for D-Ala. Under optimal conditions, the ECL intensity had a linear relationship with the logarithm of the D-Ala concentration in the range from 1.0 × 10−9 to 5.0 × 10−3 M with a detection limit of 3.3 × 10−10 M (S/N = 3). This study provides a new method for the determination of D-Ala in food with high sensitivity, excellent selectivity, and good stability and reproducibility.
研究领域:
分析化学
·
生物医学工程
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DOI
高影响因子
ChemNanoMat
·
2023
·Vol. 9
·期 5
·e202200569
·
IF 3.8
Yanyu Li,Xiaoyue Zhang,Jinglin Shen,Wei Qi
Graphical A new type of nanocomposite enzymes has been developed, composed of Pd−Pt nanoparticles, hemin-rGO and CNTs-COOH. The nanocomposites show a high peroxidase-like activity and a linear response toward H 2 O 2 in the range of 0.05–500 μM with a determination limit of 0.01 μM. Based on this, a paper sensor was constructed for H 2 O 2 detection in real samples. Nanocomposite enzymes are attracting intensive attention worldwide. In the present study, a novel colorimetric H 2 O 2 paper sensor was developed based on a new type of nanocomposites composed of Pd−Pt nanoparticles, hemin functionalized reduced graphene oxide (hemin-rGO) and carboxylated carbon nanotubes (CNTs-COOH). The proposed nanocomposites show a high peroxidase-like activity. It provides a sensitive platform showing a linear response toward H 2 O 2 in the range of 0.05–500 μM with a determination limit of 0.01 μM. Based on this, the composites were applied to construct a paper sensor for H 2 O 2 detection in real samples. The cost-effectiveness, simplicity and efficiency of this paper sensor could be developed for “point-of-care” analysis and other related areas.
研究领域:
生物化学
·
分析化学
·
材料科学
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DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Guiyin Li,Shengnan Li,Qing Huang,Xinhao Li,Zhengyu Zhang,Jintao Liang,Zhide Zhou
Atherosclerotic Cardiovascular Disease (ASCVD) is one of the major fatal diseases for humans and the fatality rate is increasing rapidly. Low density lipoprotein (LDL) is a serum key marker in the diagnosis of ASCVD because LDL level is associated with the prevalence of ASCVD. Herein, a light addressable potentiometric sensor (LAPS) was constructed for rapid detection of LDL using reduced graphene oxide-polyaniline-hemin nanocomposites (RGO-PANI-HNCs) and low density lipoprotein aptamer (LDLapt) as sensitive membrane of LAPS. Under the effect of bias voltage, the specific binding between LDL and LDLapt, and forming the LDL-LDLapt complex will cause the voltage change on the sensitive membrane surface of the LAPS system, which will cause the change of original current–voltage (I-V) balance in the circuit, resulting in the shift of I-V curve. The correlation between the potential shift of LAPS and LDL concentration was linear from 1.0 to 100.0 μg/mL under optimal conditions. The sensitivity is 4.7671 mV/μg/mL, and the detection limit is 0.79 μg/mL. Also, the LDL biosensor showed satisfactory specificity, selectivity, reproducibility, and showed a good precision in real human serum samples, which provides a simple and effective way for the early diagnosis of ASCVD.
研究领域:
生物医学工程
·
分析化学
·
心血管疾病
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2014
·
IF 3.84
Hao Lin Zou,Bang Lin Li,Hong Qun Luo,Nian Bing Li
Graphene oxide (GO) has been demonstrated to be functionalized by hemin without any other reducing agents and the hemin functionalized graphene oxide (H-GO) hybrid nanomaterial was obtained under the condition of sonication, which could be ascribed to the π–π stacking and cation-π interactions between GO and hemin molecules. H-GO composite, characterized by scanning electron microscopy and UV–vis absorption spectroscopy, was used for preparation of the modified electrode considering its great electrocatalytic activity, large surface area, and abundant active sites. Due to the unique structure and excellent properties, H-GO modified glassy carbon electrode (GCE) showed high electrocatalytic activities for the oxidation of ascorbic acid, dopamine, and uric acid, achieving the simultaneous determination for those three different kinds of biomolecules with detection limits of 0.3, 0.17, and 0.17 μmol L −1 ( S / N = 3), respectively. In addition, the proposed biosensor exhibited high selectivity and good stability, which could be applied for the electrochemical determination in practical samples with satisfying results.
研究领域:
分析化学
·
生物医学工程
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DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2018
·
IF 8.173
Chunyan Zhang,Shuan Zhang,Yilei Jia,Yueyun Li,Ping Wang,Qing Liu,Zhen Xu,Xinjin Li,Yunhui Dong
An ultrasensitive sandwich-type electrochemical immunosensor was designed by using gold nanoparticles (Au NPs) as the substrate material and microporous carbon spheres (CS) loading silver nanoparticles (Ag NPs) spaced Hemin/reduced graphene oxide (Hemin/rGO) porous composite materials (Ag NPs@CS-Hemin/rGO) as the detection antibodies (Ab 2 ) label for detecting carcinoembryonic antigen (CEA). The Au NPs with good electrical conductivity and biocompatibility could accelerate the electron transfer on the electrode interface and enhance the load capacity of capture antibodies (Ab 1 ). Hemin is peroxidase-like substance which has excellent catalytic ability for H 2 O 2 reduction but easy to molecular aggregation and oxidative self-destruction. Reduced graphene oxide (rGO) is a good supporting material for Hemin to mitigate this disadvantage. CS loading Ag NPs (Ag NPs@CS) as the spacer inserts into Hemin/rGO sheet can overcome the irreversible stacking of rGO, and form complex porous structure which exposes more active sites of Hemin. Moreover, Ag NPs loaded on CS also has catalytic ability for H 2 O 2 reduction. Thus the Ag NPs@CS-Hemin/rGO used as the Ab 2 label has a large working surface area and high utilization rate, which heightens the catalytic ability for H 2 O 2 reduction to amplify the current signal effectually. The current signal and the logarithm of CEA concentration presented a wide linear response range of 20 fg/mL to 200 ng/mL, and the detection limit of CEA was 6.7 fg/mL. Furthermore, the designed immunosensor exhibited a good reproducibility, selectivity and stability, which confirms a broad development prospect when applying it in early clinical detection.
研究领域:
生物医学工程
·
分析化学
·
纳米技术
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Tingsong Ye,Rongwei Cui,Xin Zhou,Yingying Huang,Xin Li,Sami Ramadan,Bing Li,Lizhou Xu,Danyang Li
The existing aptamer-regulated nanozymes for colorimetric detection primarily rely on unidirectional activity modulation. The role of salt ions in regulating nanozyme activity and the underlying mechanisms remain poorly understood, limiting their broader application. Herein, we report a novel bidirectional regulation strategy using a hemin-functionalized oxidized carbon nanotubes (Hemin/oCNT) nanozyme. Contrary to convertional approaches that seek to mitigate salt interference, we here leverage the salt-induced charge screening effect that high salt suppresses the peroxidase-like activity of Hemin/oCNT, yet paradoxically promotes aptamer binding to Hemin/oCNT. The formed aptamer/Hemin/oCNT complex exhibits restored and enhanced activity in high salt, attributed to improved dispersion, facilitated substrate (3,3′,5,5′-tetramethylbenzidine, TMB) adsorption and accelerated electron transfer. This bidirectional mechanism was harnessed to develop a one-step ‘mix-and-detect’ aptasensor for adenosine triphosphate (ATP). The assay achieves a wide linear range of 0.625–50 μM with a low detection limit of 300 nM (UV–vis). Furthermore, a smartphone-assisted dual mode (RGB/CMY, Red-Green-Blue/Cyan-Magenta-Yellow) platform was established, where the R channel achieved a superior detection limit of 190 nM, and the CMY mode possessed excellent precision analysis (RSD ≤ 2 %) owing to its signal stability. This sensor showed high selectivity for ATP and successful application in spiked diluted serum. This work represents a paradigm for designing high-performance biosensors by exploiting, rather than avoiding salt effects.
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高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Tianjiao Liu,Deming Dong,Yingyi Meng,Haijun Chen,Chunyue Liu,Zihan Qi,Anfeng Li,Yang Ning
Due to the very important role in physiological process, a simple and sensitive hemin detection method is necessarily required. Biomass-based carbonized polymer dots (CPDs) have been widely studied especially as fluorescence probe owing to the advantages of low toxicity and the variety of fluorescence color, yet there are still challenges in developing their multi-color emission property from the same raw materials. In this work, red, white and blue emissive CPDs derived from chlorophyll have been synthesized via hydrothermal method. Then white-emitted CPDs (white-CPDs) with the Commission International d’Eclairage (CIE) coordinates at (0.34, 0.32) were used to develop a fluorescence quenched sensing system for hemin determination. There is a good linear relationship between (F 0 -F)/F 0 and concentration of hemin in the range of 0.1–0.95 μM with a detection limit of 0.043 μM, and the quenching mechanism was considered to be caused by inner filter effect (IFE). Moreover, it has been successfully used for hemin detection in serum and also for visual determination, which indicating great potential in applications of disease diagnoses and trace identification.
研究领域:
分析化学
·
生物医学工程
·
材料科学
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产品关联
DOI
PubMed
高影响因子
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